專利名稱：人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽i組的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物技術(shù)領(lǐng)域，尤其涉及一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽。
背景技術(shù)：
肝臟是人體內(nèi)最大的消化腺。也是體內(nèi)新陳代謝的中心站。據(jù)估計(jì)，在肝臟中發(fā)生的化學(xué)反應(yīng)有500種以上，實(shí)驗(yàn)證明，動(dòng)物在完全摘除肝臟后即使給予相應(yīng)的治療，最多也只能生存50多個(gè)小時(shí)。這說明肝臟是維持生命活動(dòng)的一個(gè)必不可少的重要器官。肝臟的血流量極為豐富，約占心輸出量的1/4。每分鐘進(jìn)入肝臟的血流量為1000-1200ml。肝臟的主要功能是進(jìn)行糖的分解、貯存糖原；參與蛋白質(zhì)、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防御機(jī)能；制造凝血因子；調(diào)節(jié)血容量及水電解質(zhì)平衡；產(chǎn)生熱量等。在胚胎時(shí)期肝臟還有造血功能。
肝臟疫病分為肝炎、肝硬化、脂肪肝、肝癌等?，F(xiàn)代醫(yī)學(xué)實(shí)驗(yàn)證明，肝病病毒侵入人體后，并不直接引起肝細(xì)胞的損害，只是在肝細(xì)胞內(nèi)吸收營養(yǎng)賴以生存，并在肝細(xì)胞內(nèi)復(fù)制、繁殖。其復(fù)制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細(xì)胞膜上，引起人體免疫系統(tǒng)對這些抗原物質(zhì)產(chǎn)生免疫反應(yīng)，這種反應(yīng)造成肝細(xì)胞的損傷、壞死。免疫反應(yīng)的強(qiáng)弱決定于肝臟受損程度及臨床癥狀輕重。這場由病毒引發(fā)的、免疫系統(tǒng)對肝細(xì)胞的戰(zhàn)爭，使大約25％的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場，肝臟的損傷由此加重。肝病的危害絕不僅僅限于肝臟本身，它還可以引起其它多種疾病。常見的有(1)糖尿病；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎病；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結(jié)節(jié)性動(dòng)脈炎；(12)消化性潰瘍；(13)自發(fā)性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對患者的身體甚至生命造成危害，而且對患者心理上的打擊也是十分沉重的。無論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學(xué)等方面都會(huì)受到嚴(yán)重影響。
生物基因組中可轉(zhuǎn)錄表達(dá)的序列(即基因)僅占總序列的3-5％，對這部分序列進(jìn)行測定，將直接導(dǎo)致新基因的發(fā)現(xiàn)，并獲取基因組中與產(chǎn)業(yè)化關(guān)系最為密切的信息。20世紀(jì)80年代，高通量的自動(dòng)測序的出現(xiàn)，使從質(zhì)?；パa(bǔ)脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫隨機(jī)選取許多cDNA克隆和決定來自非載體兩端的幾百個(gè)堿基的DNA序列成為可能。這些短的DNA序列叫做“表達(dá)序列標(biāo)簽”(Expressed Sequence Tags，簡稱ESTs)。表達(dá)序列標(biāo)簽的概念最早是由Adams等在1992年提出來的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela，et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.Nature Genetics.2，173-179)針對獲得大量信使核糖核酸(mRNA)序列的迫切需要，提出大規(guī)模互補(bǔ)脫氧核糖核酸(cDNA)測序的研究戰(zhàn)略。隨后Venter創(chuàng)立了大規(guī)模表達(dá)序列標(biāo)簽技術(shù)。其基本特征就是從以質(zhì)粒為載體，構(gòu)建完成的目的組織互補(bǔ)脫氧核糖核酸(Complementary DNA，簡稱cDNA)文庫中，隨機(jī)選擇許多cDNA克隆，利用質(zhì)粒上攜帶的通用引物對cDNA兩端進(jìn)行一輪脫氧核糖核酸序列測定，所獲得的來自3’端或5’端的幾百個(gè)堿基的非載體短脫氧核糖核酸(DNA)序列。簡而言之，表達(dá)序列標(biāo)簽是來自表達(dá)基因片段3’端或5’端的短脫氧核糖核酸序列，代表一個(gè)表達(dá)基因的部分轉(zhuǎn)錄片段。
表達(dá)序列標(biāo)簽可用于新基因克隆、人類基因組圖譜繪制、基因組序列編碼區(qū)的確定等。如果一個(gè)表達(dá)序列標(biāo)簽在基因組中只出現(xiàn)一次，那么它可以作為序列標(biāo)簽位點(diǎn)(STS)。由表達(dá)序列標(biāo)簽構(gòu)建的物理圖譜叫表達(dá)圖或轉(zhuǎn)錄圖(expression ortranscript map)。利用表達(dá)序列標(biāo)簽進(jìn)行基因圖制作，可以加快序列標(biāo)簽位點(diǎn)的制作和新基因的染色體定位。表達(dá)序列標(biāo)簽可以作為基因特異性探針，對組織特異性基因表達(dá)的研究具有重要的作用。表達(dá)序列標(biāo)簽還可以進(jìn)行新基因的遺傳進(jìn)化關(guān)系分析。表達(dá)序列標(biāo)簽可以對所有動(dòng)植物的基因作為一種數(shù)據(jù)庫，通過不同的序列比較可以獲得保守序列片段，從而獲得基因的遺傳進(jìn)化圖譜。正因?yàn)楸磉_(dá)序列標(biāo)簽具有如此的優(yōu)越性，因此表達(dá)序列標(biāo)簽測序已經(jīng)成為許多基因組研究機(jī)構(gòu)的工作重點(diǎn)。
表達(dá)序列標(biāo)簽(EST)是一種快速有效揭示基因組容量的方法。ESTs在新基因的發(fā)現(xiàn)、基因作圖和基因組序列編碼區(qū)域的確定等方面起到重要作用。EST不僅為基因組遺傳圖譜的構(gòu)建提供了大量的分子標(biāo)記，而且來自不同組織和器官的EST也為基因的功能研究提供了有價(jià)值的信息。此外，EST計(jì)劃還為基因的鑒定提供了候選基因(candidantes)。
在本發(fā)明之前，還沒有出現(xiàn)涉及本發(fā)明的一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的公開報(bào)道。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問題是提供一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽。
為了解決上述技術(shù)問題，本發(fā)明通過如下技術(shù)方案實(shí)現(xiàn)在本發(fā)明的一個(gè)方面，提供了一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列，其包括(a)SEQ ID No.1～SEQ ID No.58所示的序列；(b)SEQ ID No.1～SEQ ID No.58所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.58所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.58所示的序列。
本發(fā)明還提供了一種探針分子，所述的探針分子含有上述序列中約8-100個(gè)連續(xù)的核苷酸。
由本發(fā)明的一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽，可以方便的尋找出在人類肝臟中特異表達(dá)的相關(guān)基因，從而在研究肝臟疾病的致病機(jī)理以及開發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
具體實(shí)施例方式
下面結(jié)合具體實(shí)施例，進(jìn)一步闡述本發(fā)明。應(yīng)理解，這些實(shí)施例僅用于說明本發(fā)明而不是限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實(shí)施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來源于5個(gè)成年男性，在肝臟切除手術(shù)后，將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩后，再移入玻璃勻漿器內(nèi)，勻漿后移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑒定總RNA質(zhì)量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實(shí)施例2cDNA文庫的構(gòu)建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補(bǔ)平末端后，加含EcoRI切點(diǎn)的接頭。磷酸化EcoRI末端后，用XhoI限制性內(nèi)切酶消化1.5小時(shí)，再進(jìn)行片斷分離。過柱篩選長度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，無菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進(jìn)行包裝，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)細(xì)菌。涂板并測定滴度。
實(shí)施例3測序及數(shù)據(jù)庫建立(Seqencing and Database Constructing)挑選文庫中有外源片段插入的克隆，擴(kuò)增后抽提質(zhì)粒(Qiagen Germany)，用T3和T7作為3’和5’端的通用引物，采用終止物熒光標(biāo)記(Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377測序儀(Perkin-Elmer，USA)上進(jìn)行EST大規(guī)模測序。測序結(jié)果用FACTURA軟件去除載體序列，傳輸?shù)絊UN Ultra 450Server上進(jìn)行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group，USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(Genebank+EMBL)，將無同源性或同源性低于95％的序列視為新基因建立數(shù)據(jù)庫。
實(shí)施例4基因的全長克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎(chǔ)上，進(jìn)行cDNA全長克隆，分兩階段進(jìn)行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針?biāo)褜bEST數(shù)據(jù)庫，將重疊序列＞50bp，同源性在98％以上的表達(dá)序列標(biāo)簽(Expressed Sequence Tag，簡稱“EST”)序列認(rèn)為同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER軟件進(jìn)行連接，部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長完整性如何。通過電子克隆的方法，通?？色@取人肝臟相關(guān)基因的全長序列。
(2)cDNA末端快速擴(kuò)增(Rapid Amplification of cDNA Ends，RACE)如果通過“電子克隆”方法仍未得到完整的cDNA全長，則在已有序列5’或3’端設(shè)計(jì)引物，在人類肝臟Marathon-Ready cDNA文庫(Clontech Lab，Inc，USA)中進(jìn)行長距離PCR反應(yīng)。然后對PCR產(chǎn)物克隆、測序。用AUTOASSEMBLER及STRIDER軟件分析被延長的序列有無完整的ORF，如無，重復(fù)上述過程直至獲得全長。
(3)RT-PCR對于5’和3’端的已知的序列，如果中間有一段間隙(gap)無法從已有的公共數(shù)據(jù)庫或自身數(shù)據(jù)庫獲得，可考慮采用RT-PCR的方法。在序列5’端設(shè)計(jì)引物，3’端引物采用Oligo-dT，在肝臟總RNA庫中進(jìn)行擴(kuò)增。然后對產(chǎn)物進(jìn)行克隆、測序。最后拼接便獲得全長。
通過組合使用上述3種方法，可獲得人肝臟相關(guān)蛋白的全長編碼序列。
序列表<110>上海人類基因組研究中心<120>人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽I組<130>NP-10041<160>58<210>1<211>563<212>DNA<213>Homo sapiens<400>11 taacaagggg gcagtcattt aatgcagaaa tccagttaac ataaagagac agaggttgaa61 caacaggtct tcaaacaata ctgcatagtg tcacgagtta gctcttaagc acgtttgatc121 tgtttatctt atgatttcga ttccagtaca aagacagaga agagacttaa aaaaaaaaaa181 acaataaggc aacagaagaa gaatcttcct gtgacaagga acgtccacaa ataatgcaca241 aatatttggt gctgacacaa taagacacca tcaggacaag ttctgaagat gctgcattaa301 aatttaacaa gcatgataca atgacaagaa aattatactc gctttgttac gaatcctttt361 gcaagtagaa atgactctcg ggtaattgtt acatacaaac tttctgaaat aaatggaaat421 catacaaatt tttccattgc agtttctctt tctctacacc tagaaatgtc catggcactg481 ctccctattc tcacagtcac gcataatagc cttaaagcct tgaatccaca gtggcttgtc541 cttagaaggt ggaggttgct gat<210>2<211>659<212>DNA<213>Homo sapiens<400>21 acattttaga gttgtttact tttattaata aaaagttaga tcacttatat atatatacat61 tgaaaaaaac tattattgtc cctttcctgg tgaaaaggag tatacacaac agacctttgt121 gatattctgc aacaatgacc catattgctg tggaattctc actttttagt agaaccttta181 aaaactcaag tgagctgtac aacgccccga ttgctttaac tccagtgaac cacggaggta241 taaagacgct agagttcttt gtaattcttc agtgccaaaa ataataaagt tggaaacttg301 gattcctgtt tgactctact gtattaagtg atcagaataa aattactgta aggctaatct361 tcaaccactt catggttttc attcttgctt tctatagtga acatctggtt gtttccactt421 ggctctaaac ccctttcctt ttgggattca gcccactact catccatgtg attctgacag481 gaggttagca atgacagtgc agatagatag cccaggcctg gccaaagatc acaggcccca541 tcccctcttc cacagggact gggtccatgc aagggcaggt gattctgggg aagaccagag
601 tccttttata ganttgattg tggacctgaa aaggaagtnt cccctccctt tgancatgg<210>3<211>604<212>DNA<213>Homo sapiens<400>31 tnataaacat atngaaaact ttantagaaa tatanagaaa aanaaataac ttgcctgnga61 ggtttcctgt cccttcagga tggatganca acagatgggg cacctaggtc cagtggggct121 gctcccgatc tactgtttct tcctccagga gcaaggcatc tagctccttc cagccgctnc181 tgagcagggg gcccacatca gggctggggg ccacattggg aggaacagtc ctgccccctg241 cagcccggac cccacggacg gtcttcgtga tcgaggaagg ctgctgtccc gaaattccac301 agcccgccgg agcttccttg agctggtgaa aatcagagtc ccattgcgct cccggggttc361 ctcaaagatg gtctcaaagg tcctcctggt tgtggggtga ttggtaattc ttggttggtg421 taaatttctt ccaagctgaa ctccttcttg gttcaacctg attgggtcga ggcagtccca481 ttggtgtaan gtttaagctg aggacgggct ggtgttctgc ggnattctga atcgngaacc541 tttcccatgg tcttggtctc aaggttngaa agcacctggg tcncttgaag gctcttggct601 cctt<210>4<211>629<212>DNA<213>Homo sapiens<400>41 aacaaattgt attattacac attcctagtt gctgcttgta atagaacaga tttcaatctc61 agaaatgttt atctgagaga catagatcag gtttacaagg acaagaggtc aaagtcatgc121 cacagacgat ccacaccacc tatgtatgca ctctctctct tgcacacaca cacgcacacg181 cgcacacaca cgctgagtct catgtgctta cctcgtctga ctgccgctgg tgttatatgt241 agcttccatg cctggaagag gcacatatgg taacatggtg tggctaaagt aaagcaaaag301 tacaaaagca ataggtaagg gcagtgggat aacaggaaag gttttgatgt tattgtgggt361 attagcacat gatagcccca agaagacaag aaatgtcagt tactcagaaa gaggaaagct421 ttccaaccag gagagttata tttatgtgcc ctcactggaa tggaggcaga ctgacaggaa481 gattattttc caacttgaat ggaaagaagg cagagacaga aagaaagaac agtggctggg541 caagtggctg atgccnataa tcccagcact tgggaggctg aagttggcaa tcgcttcagc601 ctggagttca gacccagctt ggcacatgg<210>5<211>716<212>DNA<213>Homo sapiens<400>5
1 agatggagtc tcgctctgtt gcccaggctg gaatgcagtg gtgcgatctc ggctcactgc61 aacctcctct tcctgggttc aggcaattct cctgcttcag cctcccaagt agctgggact121 acaggcgcat nanccacgcc gggctaattt ttgtattttt agtagagact gggtttcacc181 gtgttagcca ggatggtctc aatctcctga cctcgtgatc cgcccgcctc agcctcccaa241 agtgctgggg ttacaggcgt gacacncgtg cccggctgag aacattttac atatatgcga301 aatagctacc cagtcctgat tcgactagag cactgagtga cacacattaa atgttaagtt361 tgaaaagact gtaaaacatt cacatatgac agctcttagt gaagtaagag cctctcatat421 tncctaattg tttgaaacat tccaggagag ctaggttccc gtattctcct ctagaagtgt481 gtatagggct ccctcccttg tttggngata ttnttgatat ttcctccgaa tgatgtttac541 ttgactctgg ggcaagctga gcttttccac agatttagtt acttcttcac taactcactc601 ntcagcaagg ggaaactccg atgaatnagt tcngtattgg naactggtag gttgttanca661 attatttcaa gttccgatgt atccgaatgg gnaagtgagg nccgtattgg aattct<210>6<211>392<212>DNA<213>Homo sapiens<400>61 tttgcaggtg actacgcatc atggtaagtt ttatacccta gtcaaaatga aaaatagcaa61 aaaccgcact gtaaaaaaaa aaaaaaaatt ggtttccttt agtagatagt atttcaaccc121 atcatattct tattataaat aaacagaaca atcttaagtt gacagatatc aaagagacct181 tgatctgatt ttggtgccaa gtgaggtctt aattcctcat tcctgcctta agtgcttggt241 gtcatacagt gggtggtaca aagctgtttt atgcctaata ttgctaaatg ggacccttct301 aattgatttg aaagacatta taacatattg tccagacttt aagacttaaa gagtaggaaa361 tactactttt agtggctaaa tttattcatt tt<210>7<211>488<212>DNA<213>Homo sapiens<400>71 gatcccagca gtgtccagaa gaagaccaag gacagaacag agactaggtt tggtgagatg61 ggacagattt tgggaaagat catgatgagc catcaaccgc agccccagga agagcggacg121 ccccagcggc gcctcagggt accccctcca ggaggtggtg gatgatgaag tgttgggacc181 atcaggtgag gggactggag aaagaagagg tggcatagga ttgactaaga tgaaggaagg241 gggccaggcg tggtggctca cccctgtaac cccaacactt tgggaggctg aggcgggcag301 atcacctgag gtcaggaatt caagaccagc ctggccaaca tggtgaaacc ccatctctac361 taaaagtaca aaaattagcc aggcggtagt ggtgtgtgcc tataatccca gctacttggg421 aggctaagac aggagaatca cttgagcctg ggaggaagag gttgcagtga gccgagatcg481 tgctactg<210>8
<211>569<212>DNA<213>Homo sapiens<400>81 gggtgaaatg agttactaaa tgtagcattt atttataagg aatgcattgt gaatagtttc61 tcagttttca ttatggaaag atgatgattt cagcccacat tcagtgtatg tttctaaata121 acacaatcga caggactgtc tgttcagtac aatggaggac agctttttca gggcaaatgg181 gatttcttga taatgctaaa tctgtcttgt cagctgaatt tcttgggctt tatgtggcag241 tgtggtaaaa atatatgatc agatttcact gttaagaaaa ttctttcagc aatacatgta301 gagtcaagtt tcttgcatgg ataactgaac atgtgggtta tgagatttta aaaaatgtct361 cgtgacaaac tttacggaaa tgcaacaatc tggacatcta gttttgtctg agagtggcgt421 ggatatgaag aactgtgctg ttggtgctga tgccacacta agttttggca gtcacactct481 tggttcttca tatttgagga gatgggatgg tgaggaggcc tgttggcttt attttattaa541 tgccaccatc tagaatacag attcttgga<210>9<211>581<212>DNA<213>Homo sapiens<400>91 ggtaaagact tttgtatagt tttcctgatg ccctaatttg ttccatttaa tttctataaa61 tattcaaagg acatggaata ggatctgtta aatcaacttt attaatccca ttttaaatat121 ctttcatatg cttatttttc tgcttgatca gcaaataatg aactaatcac tttcaaactg181 ctagtttcta tacattcaat tatctgagat ttctattggc ttttacattg tttgatatta241 tacgttttca aataaatatt catataaatg tctataggtc ttatagttct gttgtaggct301 acacatgaac agcaaccaaa aagaggaaaa atcttctttg tccccatgct ttttccttga361 attctgcact tttcatatta acattagccc ttgctttgtg atttgccttt gctttatttt421 accctgtctt aatcttaacc ttcccaaata tttccataca gggtgcacct ctcctagaca481 acacacagcg gagagtgtgc ttcccatgta ttcaatcttc gtctcttgaa aggagaggtg541 ccctcattac acttcactgt gacacaggat acccttggtt t<210>10<211>442<212>DNA<213>Homo sapiens<400>101 ttgagtatga atagggttta ttaggtcttt tataaaaata ttcactaact tttctctagt61 ggtctcatct tctattctgt aaatggctct tagaaggaaa tgttttccag gtacagatac121 ctaaatgggt atctatctca caatggcctg gagttctgtt ccctcctcat tccttaatga181 gtgcttgtcc tgctgtgccc ttgcattgtt taatttgttg ctccttttta cgaaactcag241 aaattcttga tccataaagt agggcttttc tgacgatcca tcatttgttt ccagatcaac
301 agcaaaacac aggaaaagtg catccagcac agtttcaaac acagataaaa aactatgggc361 tactaagtag gcaaaaaaag ctaccaataa cagagggact gcccgcacct ggaatgcccg421 attgtagtta aaagccatga gt<210>11<211>559<212>DNA<213>Homo sapiens<400>111 ttttcttaat tatttctttt tattttaata aagcaaaact acaggtaaat gttttgccag61 atgacttttg tgtacttatg ttctaacata gcacatgttt ggcaccaaaa gatcttagaa121 actactatga aaaaccataa gattttaagt agttttctga cagaaatgaa catttctgtt181 gcatccctac ttgtcactgg ctttcttaaa cacagaaata ttcatttcca tagtctttat241 cgctttttgg aggatctcta agattggtcc catagccttg acaatttgtt ccaacaaatc301 tgctgctgac tttgtgggat ttttggaatc ctcagttttt agtacatcct gcagtttttt361 caaggtgtct atcagtggtt ttggaagaac ctggaggact tctggttctc tcagatgtgg421 tgacatctga ttgctctttg gtgtcttctg tatagaagtc acttaattga agattcatga481 tggggaattt catcaagtgt gaaagagaag attccagact cccaaggatg ttactgctgt541 ttagcacaga gatgatgac<210>12<211>432<212>DNA<213>Homo sapiens<400>121 ttttatgtta agtcactttt gagatattta ttattagaaa ccatccaaca tgaaatatga61 attattaaaa tgtttttctc catgtttcaa accagaaaca gtaattcaga atgcaactag121 catcctaatt tttaaaatca aaataagatg accagattaa taaaacttag cattcacagt181 attcaatcta gaaaaaatat aattaataga atgttaaagc caaaagaaca tttgaaagac241 agaaagagag aaggggagga gagaaagaga gagggaaaag agaaagcaag aaagaggagg301 aggaggagga ttgagagatg gagagtttga gattgagact tcttatgaaa tatattttaa361 cggcaattac tcattaacaa ctgtgcattg agaatatttg aacatgttgt tnaaagctag421 gggaaaaatg tc<210>13<211>501<212>DNA<213>Homo sapiens<400>131 tttgaaacat gacacagcct tttattattt cagatcgtga gaccaggtca gaagggtcgt61 tacgctcatc atgatactac tgctttgaat accacacaga ccagcgacat ttcccattca
121 tttccccaac cttgtgggaa aaattttcag gagtacagca tcaatgaaga acacagtgtc181 atgaaacaca aatgctgtca cagatgacag tcacgttacc atgctgaatt gtccagagaa241 gctaaccagt tactactcag ggctgatgta caatagatgc aacgccaaaa tgagatgaaa301 gagaattcag aataaaattc cgtcccttgg agcatattcc attggctttc tctgctcctg361 cttccgctgg tatctggcct tctggtagaa aatgatgatg gttacagtga ctatatttag421 aagaatgacg agcagtatga gccagaacga gtatccgtaa ctgtgggtcg ttcctttact481 ggtggttgcc gggtanagca t<210>14<211>607<212>DNA<213>Homo sapiens<400>141 ttcagattta tttttatatt tattaagcag taagcaaaca gagttggggg agatgaccta61 aaatggtttt cttttggaat aatatttttt aaaaaatcaa ataactgtta tcactttaag121 ttgcatgagg ctgaagctgc acctggagag gaatttaatt ttcatgacat gcaaaactga181 ttaggcagtg aactctttcc tcccgcttcc cctgaacaca tttataaact tcaacggggg241 tgaattccat ctaatactct gaaaaccatt tctcatctca cagtttaccc aaatcatgtg301 catgactgca agaggaaata aacaagaaat ctttaagcat agataagcca caatggatag361 ttaatcttgt acagacaatc cagcattaag gctaatttga tcttttaaac attttatggg421 aaacagctgt tgagttttct ctagaaaagc ccacaaggtt ccataacccc ataggcatac481 agcctcgaca ggcagggctc catcaggctc ctcttgaact gggcactcct tcacggaagg541 caggaatgca cgtgtcttca cttcctgctg ctctgcacgc acgtcacgat atctggcaga601 aggtggt<210>15<211>502<212>DNA<213>Homo sapiens<400>151 caattcttaa tcatttattt tcaacacgtg tactataaag aacctaagaa atcagacaat61 tttaataacc cataaacatg ttgaatccat ttgcagattt aaattttgta aaaatagctg121 tcactgcctc catatatcaa gtgtactatg tttataatca catttatgct accgatactc181 ctcagaaaaa aacagattct gcttggttct agcttcagta tcatgaactc caataatgcc241 ttgaggacct ccaaaggaaa aaaacagatt ctactaggtt ctagctgaaa tattatcaac301 tccaaattga agctgaagta ttatcaactc caaataatgc ttcgaggacc tccaaaaaaa361 agagattctg cttggttgta gctgaagtat tatcaactcc aaataatgct tggaggacag421 caatgtagat gattttgtaa tcacctttca tcggagtgac cttatataaa aaataagtca481 ataatttaga ggttctaagt ct<210>16<211>576
<212>DNA<213>Homo sapiens<400>161 tattcacccc tcccttgaag gtttaataga caacaggaat ctgggctctg aaaagtcaca61 aacacctttc ccaagatgat gagcaactgc aacaactggc caaatgtgtt agcagcagaa121 aagctgggca ttacctgtgt gagaggcgct cagcatgggc agtgaatggc cgtgagcagc181 gcaccagcta catagcctcc acaccggaag ggtgctgctg ctctgcaggt ccgtttcagg241 cgggatccag ccatccacag gaccaagggg ctggagtgtt gggaacagat gattctgtgt301 tccacagata atggtacaaa gtgactgttc atagataagg tgtaagcaca gcatttgaag361 acagctcctc cagatggtct gtctgttagt gaattcttaa gcacttagag ccttgaagat421 tgagaaattc taaagaaatt ttttaaaagg aggagggtgg tagatgtcaa agacacaaaa481 gctctttgta ggtgctgact ggctcacttt gtccccaggt tcatcttaga gctcaagcag541 attcaagaca tgaggcagag atgctagact tgcgtc<210>17<211>450<212>DNA<213>Homo sapiens<400>171 ggctagggcc agcctgctgc aaaccctaga gttcacaaca aaaccaccag ctctgcctac61 agagccagtt ctttccttcc acacccacat gagctcacat accttttcca tgggatcctc121 ttacagctgc tttcattcca gctaccagat ggggacttgt atgtggccga gcataaccgg181 ctgtgaacaa gcagcactct gtctggggag cccagttcag catctgaacc cccaggctaa241 agggagagct ctggagccaa cacagcactc agtggtcagg aagggggacg gtgcggcggc301 ggcactcgct gcttctcacc tggcacgggc ctgcaatctg ggtcactgtc ctgagtgcgt361 ggatatctca taaaccaaga gccatgcctg tgcctcgggg tgtggttgtg tacacgcggc421 cacaagtgca cacggctaat ccagttttgt<210>18<211>562<212>DNA<213>Homo sapiens<400>181 agagatttac aaagattttt atattaattt aataaaacaa attcttaaat agaacaaaat61 aaacacctaa tgagccactt atatatagaa tgcttgtgcc cgttggctct cgttcttacc121 ttgggccctc ttacctgcca aagcctacaa ggcccttaat tctaaagcca cccacccttc181 ccacaaccat aaaaacgtac agtccagtga ccttggaatg caggatctgc cagaggcctc241 cactacgtgg gcattaacac cctggctggg gtggggccct gtactgtagc cttggccttt301 cattcagaca gaaaggctct accttctttc ctccccaaga agagacactc cctcatgact361 gtcactcact ggcacaattc ctgcctctca gcaggcatca gctgacaact cccggccacc421 cctgtggccc tcttgtggcc ctctctagaa gaacatgaag tcccagctct gtggcaacca
481 gagccatcct ttcttccacc tcccgctcag agagctctgc gtgaatgggc agcccggctg541 gctgtgaggc gccagcacac tg<210>19<211>492<212>DNA<213>Homo sapiens<400>191 ttttatatta agcaataaac tattcggttt attttaacat ttgagaattt cttttgtata61 taaatgacat taaaagattc tcagaagaag aaaacctaag ctgaatggat taaaggctgt121 ctgctgaggt gctctccccg ttggcttcag aaatggattt tggttaggta taataagttg181 tggagtcctg cagaggttct ttacacattt ctcttctttt gagcagagag agcaagattc241 tcctcttacg taaggaggca tatttgcaaa atttcctgca ggtccgtagt tgcatacaaa301 tattgcagtt gaagctcccc caaggttagg acacattgca actgcacaac cgacataaaa361 tgaattggcc caaactaact gtgtataatg gccacagact ctggagcatg atagactatc421 aaaatcataa aattgggttt cattatacca agccgtaatg gcatgtcttg gtgtgaatga481 ctttattcca cc<210>20<211>528<212>DNA<213>Homo sapiens<400>201 tttttttttt ttaaaatata aaggaatttt atttatgaat gtatagatgt acagcaaata61 ggaaagataa tacattaaaa gaacatatac tataaccagg tagagtttta tctcaggaat121 ttaaggatgt tttaatatta caaaaaccct tattataatc atattaggca atttaagtga181 taaaaatttg attgtatcaa aagacaccaa ttccttttat caagctttta gcttccaaca241 aacaagaata gaagatgtta tttctgttat cctaacaaag aacatttgtt taaaaccaac301 aattaacttc atatgtaatg aaaaaatatc aaaaaaactt attacaattt tatgaaacac361 aaaaacaaaa tagaaatacc tatgatttta tatttagaaa gagcaaacag gccatctaaa421 actgttagaa caaagaaaat caccaaaaga tacatatttt aaaagttaat agctttctaa481 taatagagat tcccatgctg acagcaacat agatataaaa cagccaag<210>21<211>443<212>DNA<213>Homo sapiens<400>211 tttttaagta agcacagcaa taaagtttta atggaatagc tttggcccag gcagaggcag61 ttcccagatg tctcaggacg cgtgtgaacc ccaagaagtt gggggcgtta tctgggccct121 tgggatccat tcccctgggt ataaagtgtt ggtacgtggc tccaggcctg gcccactcag
181 cctcccccag gaaggcttct caagtggaga aggttcttgg cctcagaact cacggcgctg241 ctgctcacgc tgacccccca ctagatgcag atcggtgtag aaaggcctgc cagggcaggg301 atgagggccc caagctggaa gcagcccaga aatcctttta ccagaggaca actccctaat361 ccgctgggca ggggcggggt gtcacacgca ggattccgcg acgactgtca ctgcctcctg421 ggcgcccctc tgtgcgggag cgg<210>22<211>474<212>DNA<213>Homo sapiens<400>221 tttgttattc agaatatttt attttaaact acaaattagc acagctaatt tgaatattta61 aatttccgta agcactgtaa attgaataca ttttttctta atgatatagt ttttttccta121 aaaatcctag gcatattcag acttaattca aatgggttgt ctaaaaccag acaatacaga181 tgtggagcca tttgtggtag atggacgtgt atatcttgca gaagtctcca atggccttaa241 aaagcattat tattggacat caacttatgg agaatcttat gaccactcat ctaatgcagg301 ttttgctctt gttccagtag gtcaacagcc atgaaaaagt agctgtaaaa taattttcat361 acacttgtct tattgtttca cagagattaa tattctatag cctattttct tttataaatt421 aaaacaagtt acttttttca aatgtgtaaa agataatgac tgtattaaac tttg<210>23<211>266<212>DNA<213>Homo sapiens<400>231 tttttttttt tttttttttt tataaaccac cacagagctt acaaaataaa caaaagaaaa61 attgaaatta taagttcaaa gagtaggttt taatttaata agctattggg tatttagggg121 aaaaccaatt aaacttggaa ttgcattaca aatttgaaat tgtaatgaag caagtagctt181 ttatgaaatt ggaacataaa tataatatca acaaaaacat aagaaattcc tattccaatt241 aagtctcaaa atatttgttt tacagg<210>24<211>545<212>DNA<213>Homo sapiens<400>241 ttttttttaa gtattgctca cttttactgt tctctcttag ggaagggcat tctcaaaacg61 tttccgcaca agcagaccat cccttttatt ttccccgtct gtctcctttc cttctgcttt121 caaaatgtct caagagtatt tacaagagtt gagcaacaca ggcatcttta tctggggtct181 ttatccacag agcagaggac aggaagtcat cactacagag acgaagcgat gtatggtttg241 acccagtgga ggactttgtt aaggtggagg tgtgagtgtg gagtgtaaac gtgggacatc
301 cagggcagtg gagggtaacc actggagagg aagtctgggg accagtttgg gagcagccaa361 atataaaata agcatttctg ttctaaatcc aaatgaacat tctacgctgc tgtcatctcc421 agtatacctc agagatccta aagtttcctg aagttgtttg gaagtcacgg ccgatgtaaa481 gtgaaagcgc acacatatct gtaagctctg agctcaggat ttcacgtgag gtcctttgtt541 cggga<210>25<211>724<212>DNA<213>Homo sapiens<400>251 aaaccttaac ccagagttat ttttattttc cagaacgtgt taggaactag tacttaaata61 atctcaagtc cctgaggggc cagagatccc accatgcaaa atagcaaaca gacccaagac121 ttggggagag gcggtgagtg catcagaaat ggatgggtac atctgattcc caccacgcgg181 ggctcagctt agttagcagg agaccttcag actgagaaaa aatgcaagtc tttttttggc241 ctctaatatc tgggaaggat ggagggagct caggagacac agaaaagatg gcgtatgaat301 cctgtccggc ctgaacgagg ctggagttgt gcctctggat agcttcaagc actgatcaga361 ttgtcagccc ccgctgcttg aacagatgct ttagagcctc ttccagttgc cggtttgttc421 cctgaagccc cttcaccacc tgcgctgccc actcgaagta ttcctggact cgatgttctg481 accatccctc tggggtgcag cgattcaggt ccctcagatt gtacagcttg tctgccagct541 tcaccagttt ggccccgggg ctactgtggn gcgcttggct cacctgcagc ctctntctct601 ccagcttggg cagagtcttg tcatctggta cctnctncac caggcgccgc acttgtgccc661 caaagtgtag cttcaccctc atccaggtgg tgtctgtgtc ctccaccgtg tcatggagca721 gggc<210>26<211>544<212>DNA<213>Homo sapiens<400>261 aaagctgact ttatttttat ttgaattggc caagtaaaat gttccataca aggcaaacag61 ttcaacatga aatatttaaa ttaaaaataa attacttcca tcctatataa atctctagct121 gccacataca aatgaaagat accagttata aatgaaataa gttaaaataa atggttgtaa181 ggagctaggt gtggtggcat gtgcctgtca tcccagcttt caggagactg aggtgggagg241 atggatctct tgaacccagg agtttgagac cagtctgggc aacacagcaa aatcctgtct301 taaaaacaaa cgtaattgta ggatcctctg ttacgaccag cataggttac tggtttgctc361 cctaactttg ttctggactc caacatctct attgaatgag acttttgaac agtcctctgg421 gatagcccct tcttaaaaga gcacttgatg cccaggtctc caaatcactt tcagatatga481 ggatctggtg ttcagagtct aaaacaatgg ttgaagagca gagatagcaa ggtggaatcc541 caga<210>27
<211>639<212>DNA<213>Homo sapiens<400>271 tttttgcctg tgttatcgat ttattgcagc tccaatatga gtccactcct actcaaaccc61 tgattctcag gggcctgggg aaggccaccc cactaggggc cctgtcccca ccagagcctg121 gacatgggac tctctcccaa gcaggggtgt tgtccttcac aggggcttcc acgtctctcc181 cgggcctgtc cgcactctgg gcagggctgg cacctggcta ttccctcaat ctgggccctg241 gggccatggc aatgtggaat ggtccaggtg ttcatgtcag tggggcttgg ggacatggcc301 atccacgtag aagttcctgg tgatcttggg cagggtgaat tccgctcctt ccagctccgg361 tgtcagcaca atctggcagc ccagccgcga gttctcctgg aggagggggg ccatgtctag421 catgtcgtct tccctctcct cgggaggagg caggagatcc aggtggtctt cactcacata481 cacatggcag gtggagcagg ccagggaggc ttcacaggcc ccttccaggt ccaccccgtg541 gcgctgggcc aggtgaagaa cattgtcccc gactctgcca ctcacttgga tccgctggcc601 tgagcggtct acgaacacca cgttcaccac gtncccggg<210>28<211>489<212>DNA<213>Homo sapiens<400>281 tccctgtcaa tgtgttctcc gtcactcctt acacacccag caccgctgac atccaggtgt61 ccgatgatga caaggcgggg gccaccttgc tcttctcagg catctttctg ggactggtgg121 ggatcacatt cactgtcatg ggctggatca aataccaagg tgtctcccag tttgaatgga181 cccagctcct tgggcccgtc ctgctgtcag ttggggtgac attcatcctg attgctgtgt241 gcaagttcaa aatgctctcc tgccagttgt gcaaagaaag tgaggaaagg gtcccggact301 cggaacagac accaggagga ccatcatttg ttttcactgg catcaaccaa cccatcacct361 tccatggggc cactgtggtg cagtacatcc ctcctcctta tggttctcca gagcctatgg421 ggataaatac cagctacctg cagtctgtgg tgagcccctg cggcctcata acctctggag481 ggcagcagc<210>29<211>537<212>DNA<213>Homo sapiens<400>291 ttttttgtgg tggtcactgc tcagtgtaat gtgcagaatg atttgttttt tgtttttttt61 tttttggtcc ttcattgcat cctgccatac ccatgagcaa acagtttggc attaattata121 tatcactgcc accctctgaa ctttgaaaac tgccatcttc agacttggta taatggaaga181 ggctttctct ctccaataaa ccttttgctt cagggtatac tcttcggttt ttttccagat241 gtattatgta tgaactttgt actatgtata gccagagttt tatttatttt ttaaaaaaga
301 aactttttct tgataaagga ataatggtgg tctagctagt tcttgtaaaa gtgatgcctc361 ttgaaaaaaa acagtcctat tcactagctt ttagtaaaag aatcagatct tttctttctt421 gttaccttgg agtcttaaaa actgattgct aaggtgaaac aattcaatgc ataagtatgg481 agctaagtgc cttttggagg atttcttgga agagcatcta tggagatact taaggag<210>30<211>469<212>DNA<213>Homo sapiens<400>301 gctttcggtg aattaaaatg tatttggtgc cagtggtgct gaacatagaa taaaaaacag61 aaaaagggcc cagacaggga catccaggct ggaggttgga gtggagcaca gtcagatgta121 ttcacttttc tagcatgggt ttcaaagtgc gtaaggggag aaaacgcatc atggattgtg181 ctgtgcatcc tggacaccgt gcgaggtgct catagctgtg atcttgtctc ccacaactct241 cttgggtgac agacgtccac tgtccacatt ttatagacaa ggagaaaggg aagtcaaatg301 tctcgtccaa gtctacacag ctaaaaaggg gcagaactag ggtgacgctc aggcctcatt361 tagagatcgg gggttggcga gaagtggggt gggcttctgg aggggctggg agagccccac421 aaggctgcag agggtggtga gcccggagtg ggcctggcct ggtgtgggc<210>31<211>496<212>DNA<213>Homo sapiens<400>311 tttaaggggc gagagatttt aattaacaca aatggctgtg cctagtcttt aaatctgtcc61 aaagcaaatg gagtgaaagc ctgctaattc ttcagtgctt catctggcaa tgacctcaga121 aaaaagagtc tccctcctgt attatcaagc tgacttcctt tagttccttg ttgttctgtg181 tagatctttg ttcccagaat tctcacatgg tgttcatttc tgccagtagt cactctgtcc241 atagttgtaa tgcccatagt acatccaacg ttcaggtttt tctcagaaga gctcaaggaa301 atgcttggga cccaggaaga tttaaaaatg cttgcattgg ctcaaaattc tgctgcaaat361 acagtgataa tttcaggtca ttggccttct ggtagttctt caaggcttca attctcagct421 caatcacttt gccatgaaaa tcattctgaa ggatgttatt ttgttcctga tacttgaccc481 attccatttt cctctg<210>32<211>577<212>DNA<213>Homo sapiens<400>321 aattttattc catttttaat attttggtct agcaacttgt gatacataga tgacaatttt61 gtgagttttt caaatgtgtg tacagatttt tgtaaatatg actcttttgt aattaactca
121 tgtacagcct catcctgtat agtttaatga tgaatgtgca ggggacctgt ctcaggctcc181 tatatggttc ctgggcctta tagccaggtt tgtgtggcgc tcccgacttt tgtgactgac241 tggtgtcttc ccatttggac tgtggcctgg ccagagcccc ttgcatatcc ccactgtcag301 gggcagccag gactgttccc atcctcctgg aatgggggaa ccttccttat cccctgccca361 catcccctct ccaataaagc acctgtgccc tcagcaatgg cctgccatgt gctgntgctg421 ggatgtttgt attaaggtaa gtgtgggtat atggttttga gaccgggggg tgttgaacat481 tttggggaat ttattatggg gcatcttaag ggaagacatt ccttgcttct ctgaaggata541 cagcctccag ggggagactg ccattgtggg taagtta<210>33<211>528<212>DNA<213>Homo sapiens<400>331 aattgagaca gggtctcact atgttgctca ggttggtctg agtaatgata ttatcttgca61 tttatgtggt gctttacttt ttgttacaca ttcacagtaa ttaacctcat ttgattttca121 acatctctac aacagaggtt tggcaaatat tttttgagtg gctgctaagt ggcaatcatt181 ccacaaggta tttttacatt tgttacttca cttaatcctt tcaacaatcc tgagaggtaa241 gcatttgctt tacttaacag aggaggagac ggagactcaa ggaacttaaa tcttaaaaaa301 aaaaaaaacc caaaacaccc aaaaacaatt atttatgttt tacagataag agactataaa361 accaattaac aggtactttc accacatcag cttgtaagat gaaatggttt atcatcctaa421 tgttagtgaa tgacagaggt gtgcagtctt acaaatgaca cactttgtaa gtcactgagc481 aatgtttttc tgccataagg gattttgagt ccgagaaatc aggtcctc<210>34<211>577<212>DNA<213>Homo sapiens<400>341 tttatgatat catagtcttt tgactttatt ttctgagatg gaaaagtata ggcataggtg61 tttttaatag tcttcttgat gatatccttt agaataatct atcaaatggc ttctttcatg121 tttcctgatt atcagcattc atcagtgtta ctgtcagcct tgattaagtg gttgaaaatt181 tcagagaaga ataagcaact tctgtgaacc tttccccaat ccctgagaat cataatcatt241 acagaaatat tttatccttt catcaaatat ctctggaaag tagccgtgtt cgttcattgt301 taatagggca aggtgcagtg tcatagaggg ttaatccatg taaattggct tccacccgta361 aagaaatttt ctgaaaatca cgaatgtaag tcaggaaaaa accaaagaag gaaaatgaca421 tagaccattc tgctgcagta gtgatcatgt gaagcacata acctttgtcc tcggggttcc481 aatggagttt ctgttctaaa tcagtcccaa aattgccact gtgcaaaact gatgagcaag541 tcagcatcca caccagataa ccaacaacag tctgatc<210>35<211>465
<212>DNA<213>Homo sapiens<400>351 tttattttta ttctttttta tttaaaagat tcccttttta atctgttcca aagtgttgga61 agctgaacta tacaagttcc tgccacccat ccttccagta cagttttctg aggacatact121 ttttaaatgg aaaaataaca ggaggaccct ctaactgaaa ccaggtaggc ccagagtgag181 ctcctcaggc ccctgtggca gcctggccaa agctggcctt tatggagcca gcgcgtggag241 aagtctgggg aaccggggcc cttccttctg gtgaggaaag ggctgagcaa actcatgttc301 tctttagtga aatggtccaa attcagatcc aaaagcctcc attatggcat tccctggcac361 tcttcttata ctttatacat tgaaggcagc tccctctttt atggccagta taagcaggca421 aagaaacaaa ggaatataga tacatatatg tgtatatata tatat<210>36<211>698<212>DNA<213>Homo sapiens<400>361 ttttgtcaat tgagcagatt taatttccct gagattatat tccataagtg atacaaatac61 taccccttag accaggggtt ttcaaagttt gctgtgcaaa aaatcagcta ggtatcttat121 taaaatggtc cccaacatgt ctgagctgga gcttcagtgt tcacaggtct caggtgatgc181 tgatgctact ggtctctggg tcacacttta aagaggaaga cattagataa agtaaaaggg241 agtcatgang aaaagggtgc acctaaaaaa catcacataa caaaatacta aagtccccga301 gtgggagaat acaaacatgt ctctctactc tatatctaag aatcagcnnt ntggttntct361 atattnncac tgtgtctgat tataaagact atnttaagnt tatggggctt ttttaacttc421 aaanntnaaa gtgctcccga ttttttaaat aaaacacgac tatagggctt tttagaactg481 gggctgtctt ggagagtgaa ggacactttt gggagatcaa ctcttttgaa atttatttca541 ctgctaaccc tcgataatat tgcttttttg aggctaactc cagagaaatt aattggctct601 aaggcaaggg catttattat attaataaaa gnnnnngggc tcctcgaagc acttacatta661 cataaaaagt tcacacattg gaaactantc ccaaaagc<210>37<211>688<212>DNA<213>Homo sapiens<400>371 tttcttggcc tgcagcttca tttgcgagag cgccgaggca ggacacagag cacagctgtg61 ctggaagtgt ggggagaacc cggacagctc agtcctgcca gcagccgcaa agagccgagg121 ctgccaggcc catttatgtc cctcatgtct ctagattttc tcgtcaccca gcctcaaaaa181 tatatgtgtc tgcaaccctc agtctctctg ccgtttcttg attcctttcc aaacctcagc241 cggggccctg gcctcagccc cccgtccctg cgtctgctcc tcttcacagc agctgctgct301 ggctcctccg cgctcggcgc caggccctgg gtcctcagac gcatcactgt catcagtgtc
361 cgagggcagc cgggtgctgg ccacaggcct gttggggttt ccaaactctc tccctgaatc421 cacatcgaag ggatcgatct cctcctcctg ggggctctcc ttggcattgg ccaagaactc481 ctgctccgcc tgcagcacct cctccgggct gccgcaggcg cgtactttgt ccaggagctg541 cggttcaggt ccaagaagcc cttgccccat ttaaacttcc gcagcaaaat gacagcaagg601 tctggaaagc ctacgatgca gaaggtgcag caaacgcttt cacgcaggaa agtctgtagg661 ggcggccata gttcacggng ttgcgggc<210>38<211>544<212>DNA<213>Homo sapiens<400>381 ttttatgtta cactgtgcat ttatttacaa agaggttgga gaaacacaca gaatctggcc61 cccctttgca ctggggagtc accatcgtca taataaatac agtaatttta aaaaagaaaa121 aaaatactag attggaacag ctgagggtca tcaggcttcc cccattccct ctcacaggga181 gtgggggtca tgagatagaa accatcttcc ttcccctctt ctacctccga atcctccata241 attgtccagg gatcgaagtg aaagagtcct tctttccagg tgttaccaac ccctacttga301 ccctcaaaac ccccaaatca gtgctccttc ccttcccagg gcactctgca gggaccacct361 gagtcagaca cagagtctct gaccagggtg ccaaggggat atcttaaggg tccagggaca421 tggagggggc actttgtgaa tgagaggggg ccatagctgg gcaaggaaag gaggaatgag481 agggctcatc aggaatgaga gggagagcgg tggaggggaa caaacagact aggcttctaa541 acta<210>39<211>542<212>DNA<213>Homo sapiens<400>391 taaaaatcat ttttctttaa tacaaaatca cataaagaaa ggcatgttgg ctaaatcaaa61 tattcactaa atatcagtga agtcaccact ggaatctcaa tagcacattt tcctgctttc121 ttttctccct tctgctaacc attgaagacc agggtcatcc gtgggagcag atgagtagga181 cacgcgtctg cacactggag gccctggggg ttgacatggg agcaggaagt ggaccccccc241 accctgcaca tcccttctgt ttttcttgat ttcagtctca ctggcccagg ccaaatcttc301 aagggtgtct agttctgcag ccagggagaa agtgatgcca agagaacctc gtctcctccc361 tcctcagtct gctttgaagg ggaaataaat acacaggcct agtgtgtctg tgtggcacag421 ggaggtggtt ttgccaggca tcttggaagg ttgtcttcta gaatcagagc catagcctta481 cttgtggcct tggatctagg tctgtttccc cgatcgaaaa aagaacagct gttttatgat541 tg<210>40<211>542<212>DNA
<213>Homo sapiens<400>401 ttttttttag agttgtaatt taaatatttt attaaaatac atattacaag aaagctaaga61 tttacaatga acagagttat gaagcttctc aactgtcttt aaacgtttaa caacacaaag121 ggactagctg tcctcaataa agtaacacag taacaaaaca tgtaacaaat tattttaagc181 acagtttaaa acaccctata gctaaaacac tcatactatg taaatccaca taaactctga241 aagaagaggt atttttccac caccactcca aggaacacgt aaattggcat agctatatgg301 tagtctcata tatgaagata aacaaatagt aatgcattat ttagtacata attattacac361 acaaaaggta aggcacagct caagcttatc taataaaaaa tatttaaaaa tgacaattca421 taattgcaaa caaaacactg aaagaaaaaa ttcggaagaa tttaataaaa ttttgaatat481 ttacaaattg caaatgtgag tacttgttat aaaactactt acacccatgt agaatgtgca541 tt<210>41<211>624<212>DNA<213>Homo sapiens<400>411 tttgacaccc aagaccatcc tttattgtag tattagttca tggtaactgc atgaaaaaac61 atttcaggag gaatttacaa tttccagctt aaagaacttg cccaccaaca taaccaattt121 atgaaagtca attcattaaa aggtatagaa cctcttgttg ggcatgatgg caagggacaa181 agctacaact tggcctgtgc ctttggaagc tgaggcagga ggaccatctg agcccaggag241 cctgagacca gcctgggcaa catagagaat ccgtctcaac aaaaaaaaaa ttttagccag301 gtgtgctgtg agctgtagtc ccagctacaa ggtgggagga ttgcttaggc ctgggtgatt361 gaggatgcaa tgagctgtga ttgtgccacc acactccagc ctgggcaata cagcaagact421 gtctcaaaaa aaaaaaaaaa aacccaaaaa aactcaaaaa tgtaatgaat gatacccaat481 gtgccttttc tagaaaaagt tgccaaatat atctcttgga tctgctgagc atgtcctctg541 atacataagg caagcatgtt tctacaccca gtgttgatgc cagttagttt tcagaatcca601 aactgatggc agctactggt cctt<210>42<211>500<212>DNA<213>Homo sapiens<400>421 cgtggtttcg tctatttatt aaaaaatatt tgagaacaaa acctctgcct ctttgagtct61 tgctctggca tccccagcat ctctgattct ccctggtgcc cccagctcag gaagaaggtg121 gtagtgggga gagagggtca ggggggcttg gcagggatgc aggcaccatg acttttgtga181 ccagttccta gagacgcatg ggtgtagcct caggaggaaa gcgagaggag ctttaccatg241 ggaacgaagg aaagggacaa cattgggagg caaacgttgg gagactagtc cagaaacttg301 cagttgagga tacaacaggg tcctagtgtt tcctccctgg gtcagcgtag aagtagagct
361 ttttcactgt cgaaggcggg attgaagtta gagattgccc tggcagaggt tggggagaga421 gggactagga ggtggtacat gaatgtcttc tctcattctg ctaggtccct ctcagtggaa481 gatagatagg ctggtgggtg<210>43<211>475<212>DNA<213>Homo sapiens<400>431 gttaaaatat cttttaattt taccggcaaa atacatcaat gactaatata ttcccgtatg61 gaatatattc ttattggaat atattcttat atagaatata tagactatgt atatatacat121 atgtgtatat acctatatac atatatatat cttggagata taaaactaat tgggaataat181 gtcatatata tagatagtaa gcaaacattt tttaaaacat tttaaagact ccaaactatt241 catttctatc cacctataaa aaactagcaa ttaaacctgt caaatcacag ccatcttgct301 ttacgcagat tcagaagcat gagatccttt tacctttgaa actctagttt caaagctgtt361 ctaaagtgac ttggttttaa tttatatact tgcagggata agaagtagct gtagggccat421 aaaaatgtaa agaatacagt atcgtgccca tacacaactg caaacactta ttttt<210>44<211>487<212>DNA<213>Homo sapiens<400>441 ttttttttgt tctcaacttg tttaatatgg aaaaaggtca tcaaaacggt aggcaggttg61 ctgtttttaa tacattcttg ggatgttggc cattcagaca gcattatttc aaatatagat121 gagttatcca actttttttg actcaaagat agtttgctta gatttttttt ttttgagatg181 gagtcttgct ctgtcaccag gctggagtgt agtggcgtga tctcagctca ccgcaacctc241 cgcctcccag gttcaagcaa ttctcctacc tcagcctccc aagtagctgg gactacaggc301 atgcaccacc acacccagct aatttttgta tttttagtag agacagggtt tcaccatgtt361 ggccaggatg gtctcgatct cttgacctcg tgatcagccc gccttggcct cccaaagtgc421 tgggattaca ggcgtgagcc actgcgcccg gactagattt taacaagacc taattccatg481 ggattat<210>45<211>493<212>DNA<213>Homo sapiens<400>451 aactgttaaa aatgttttta ttttttagta agcaaaattt tacgaaaagt cagattcacc61 cgtggagcca gaaccttctt tctcacggga agactgtggt accagtgcgt gttggtcggg121 tggttcatca ttagtaagct cccgtgggcc agcggcagcc tgaccaccgc caccctcctg
181 gaggggcttt tcccacggga atccttatgc cggaagacaa agtctctgca ggcaccgaag241 gagacagagg caatggggct cccaggggcc agttctcttt catcatctcg gtgctccccg301 atgtggtcac agccatcttt atacctgttg atgagcacaa agttgaaggt ctgtccagtc361 accccagaga ccgtgatccc ggatgcgctc tagactgggg atccagggct ttggaaccag421 cgtgaggctg anaatgtggt aggtcagccc agcgtcgcca tacgttgcct gcttcctggc481 acactgtgcc act<210>46<211>361<212>DNA<213>Homo sapiens<400>461 ttgattttga taggaaagct ttaattggtg actaagcttt gtaaaaagga tgtaaacagg61 ggaaagaatg aaaagaataa gatttatcta cataatgaag tttgactttt tacagtaaat121 aaaataccta tttattaata tttccaaata ttaattagat ttaataatac tatatatttg181 ttacaatgcc ttcatcagaa aatttaataa ttaagaagat acttgaagtt cattcaaatg241 gcacttgcag aaatgcaact taatttctag ttctatcata tctgtctcca aaatggacat301 atatgacagc aaaaacaaaa ttctctataa atctctccat tagttacatt actatgtaac361 t<210>47<211>595<212>DNA<213>Homo sapiens<400>471 agtttttaga aaaaaagaaa aaagtttaat agcgatatag aaaattgagc acctataaaa61 ctatatggtg ggctctgggt tacattgagc ttaagtagac caagtatcta tcccacctaa121 attatcagca actccaggcc atgacccagt gtcccattct ccttcacggt ttgctggtgt181 gtagcctcct ggatacaggc aatgtatata gatcgcagag aatgtccgtt ctggcctctt241 ccttggaagg gtctaaaaac agaccttaag gagctaagtg acttccccaa agcccacagc301 tccagtgcca ctgctgactt ttgggtgggg gtgggatcag ttaaagagga aagctttcag361 tactgtcagc acatacacct gccaattaag gggctggata gagttcatct ctaggagcag421 gacctccacc cccaccctcc accccagaag ccatgtcatg ctgtttcacc aggatcttgc481 ctgaggttag atgggagaga acagctacaa gcttgggtgg agtcactcat aaacacaccc541 tgaaagctgt ggagcangga gagtgcangc tntagagggc tctncatgat ccctt<210>48<211>503<212>DNA<213>Homo sapiens<400>48
1 aataattgct tgatttattg tacagaagtg tccacgggct cacaattcta aggacatcat61 catgacagca agtaggaaaa aaaattaaag taattctttc accaattacc agtttatctc121 ttctccaatt cctttggata cgcctcctgc aagaagccga tttccagttg tatgtgataa181 tgaaagggtt tttctttctt acgttaaata caagcgaagt gattaactgg aagatagcgt241 ctgattgcga ggtaatcagt gattcagatg gtgtgggaat ggcacctggg gatgggggag301 gcaggacgga gatggaggaa gctggtgcag cctagcctgc cttgtgccaa ggacacccaa361 gggcagaggg actgagctct gggggaggac agatttgaca taactggtcc agcctcacag421 tttacaggtc ctggagggtg aggaaagtgg gagctggctg cggatgcagg acaagaaccc481 agggcttccc ttcattttgc ctg<210>49<211>535<212>DNA<213>Homo sapiens<400>491 ttttatttat ttaatttttg gagacagggt cttgttctgt cacccagact ggagtgcact61 ggtatgatca tagctcactg taacctcaga cttctgaggt caagtgatcg tcctgcctca121 gccttctgag tagctaggac tccaggtgtg catcaccatt cccagctaat tttatttatt181 tatttatttg tttgtttgtt tgtttgtttt tgtagagaca gggtctcact atattgctca241 agctggtctt gaactcctgg gctcaaacaa tcctcccacc ttggcctcct aaagtcctgg301 gatcacaagc atgagccacc atgcccaacc ttttctagtt tttaaatttt catacatatc361 tattagatgt ttatagtgta aggttgaact ccattctttg cttctctgtt gctggtaaat421 ttttgggtat atttttacga attttgcaca ttcttttaca tattggtaaa ggaacagtct481 gtgatctgga tttattcaga tccttattca gaattaccaa ttgttttatt aatca<210>50<211>397<212>DNA<213>Homo sapiens<400>501 tttttttttt tttttgttca ctagtttttc ctttattttc ttgggtatac ttaaatagct61 actttaaagt tctattatta atttctatta agaaatgctg ggtctttttt tttttttttc121 cttgattatg ggtcaaattt tcctgcctct ttgtatggct agtaattttt taattgaatg181 gtaggcattg ggaataatac cttgtaaggc atctggctta tgttatcttc ctttaaatgg241 tattgtttta tttcttgtta aagaggatta tttctgtttg aacttagtca aatgtggtcc301 ataggctggc tatttttgta aatgaagctt tattggatat agccatgccc atttgtttaa361 tattaatata ttatctggct tcttttgtgc tataata<210>51<211>535<212>DNA<213>Homo sapiens
<400>511 aggttttttt ttttttaata tttttaatgt ttaatagtta aaatttttta acaatttaac61 tttaaaaagg tcacacattt tctgatccag caatgcccca atcagattgt ttcattttat121 tattattatc aacactgtcc cctttttggc acctgtaaaa tagttccttt cgggagtttg181 gagccaggcc aggcaccgtc ggtgcatggg atgagatggg caggtttgga gctcctctgt241 ctagtgagga tcacggtctg cagagaaggg ttggcctccc cgtctcctat caaggcttaa301 agcaaggaga accatcccaa atttggttcc ttttccccta agtatcctta gaggcaatcc361 accctgtgac taggtgacta ggtgaaggac tgaggtccag aaaggagcta tcttaaacct421 ggaatcccat ttcctagtct gcagccttaa gcagttaccc tctcagacaa ctagccctct481 ccttcctccg catgaaaacc catggcttac agggatggtt gttgctttcc ctaaa<210>52<211>545<212>DNA<213>Homo sapiens<400>521 tttttttttt ttaattttga aaagactgtt gatgtcactg tggtttatta ggtaaataca61 aagtataggc tcttgcattt tttaaaaagc aaaaccaaag agattagaaa ccaagtacac121 atatcctcta gaagagaaac ataaatcagt tttaacaatt aggcacttaa aaatgtaaat181 gtaagacaac attatagaag tataaactat agttacactc ctaaattcct cctgaaatgt241 ttacaaacac aaaatcacaa gcatggaaaa caaatttcct ctttatcaaa aaaggaacct301 gaatttgaat ccaatgtgat aaaccaatga ttaaggtact ggtgggtagg aaaaaagagc361 ttctgaaatt tacatatttg attaaaatgt aagcatttca taaaactatc acaatagcat421 acagtagata tcataatgaa aaatatatgt caggaaaata atgaatctag acttaaaatt481 ttactttaca acaaggcact taacacattg aaacagtaca gtaaatttcc aaaagaatgc541 aattg<210>53<211>516<212>DNA<213>Homo sapiens<400>531 aaaacaaacg ttcttctttt aatacagcca ttgaaaagat gacacattaa gaaacatgta61 aacactgcag ctgcttaatt acaagttgca ctgcttgatt tagtacctat gcactcccag121 gtttggggac cttaagagag ggggacagga gcctcccctc actttcacag ttggatagag181 aaggcaagaa tgtgggtaga gtaagaaaga gaaaacagaa gacctggctt gtgcacagcg241 cctgagagaa tcaaagcaaa ttctgtcttc taagtcctcc cagaagaaag gtttaagatg301 ttaggaaggt tggaaaatga acgtgtgttg tcagttggga ggcaaaggaa ttgacaaatg361 cttctagtta gaatatcagc taggaaaagg agggatacag agcctgacct catgctctga421 aaaggcaagg aacccagaac ttgcctccct tatccactta agccccaacc ttgatcatca481 agggcagcgc atgggtgatc ctgttcccat gaggat
<210>54<211>571<212>DNA<213>Homo sapiens<400>541 gaatgatgct tttttttatg atcttaatta cattaaagga tttaaaaaat gccactgatc61 tcacagttta caatatccaa atcttcaaac ctgctggaag aagtccacag cacagcctgg121 aaattgcatc cgttgcattc tctcgtgcag ttaccttctg ccttgatatg tagtcagttc181 ttcctgaagg atggaagctc tcttttgcag aaaattaacc tgtgatttta gggaggaaat241 ggtgtcttca ggttcttgtc ttagggatgc tggcatcaat cctttcaatt ttgtttcata301 ttcttgtcgt atgtaagtta tctgttcctg tgactccaat tctttgtgtt gtaatttttt361 ctctgcacat cgcacctgat tagaacggtt ttctaattca tcttgtaaaa ccttgattgc421 ttggtcatta tctctaatca gctgcttctt ctcatcttca aacttttgtc taacatcctg481 gagccgcctt tctgcagcaa gctgctgctg gctgttctct tctttcagag aggaaatggt541 tgtctgaagt tctgctatga tctgtgaaga t<210>55<211>1711<212>DNA<213>Homo sapiens<400>551 tttttttttt taaatctaag ctattcccat ctcccccatt ctatgctggg gtgatatacc61 acaagaagtt acaggaacaa ctgctaaaag aattaaacat cttgggcctt tacacagctg121 ttgctatatc atggctttgg tgcatggtcg gtcacagatg ctgtcaagag gcttatgttt181 agttatcctt ttgcttcccc aacccccaca ttaaaggtct ccttcacctt ctctgtcctt241 tttgctacct cccttcttcc tgctccaagc tcccacaaac cagccttaat aaaagaggaa301 ggatcaaggc aacactccac atcagtcata tttcagggca gcttgatgtt tgtttgctaa361 tagatggttg gtattatcta accaataggg tgactccaag ttttaaaaaa cagcaagact421 aattcagaaa taatattatt tcttaatttt tttaaaaata gagatggggt ctcactttgt481 tgcctaggct ggtcttgaat gcctaggctc aagcaatcct cccaccttgt cctcccaaag541 tgctaggatt acaggcgtga gccattgcac ccgggctctc agaaatctta aaaaggattt601 agtttcattt aaaaataaca aacacagttc cccaaatctg aaatttagtt attgaaactg661 gaccatgttg tccatggaaa acatatctta ctttatacca gattttaaaa ttaacactgg721 tgtaagtgca gcctaaaatt cctctgagcc ccacttttac ataagaaaaa ttattgaata781 tgataaaggt agcatttcca atcagtgaga aaagataggt tattcaattt gtgttgggac841 tggtggatat atatctagga aaaaaaagta cagactctaa atattgaaag gtaaaactga901 aaacataaaa atatttgaag aaatcactgg aaaattattc tacaaactgc atggggaatg961 acagtctgtg ataatagtaa ccaagaacca tgaaagaata gattggtaac aattcagcta1021 cataaaaatc cctaaatttc tctatggcag aaaccatgaa acagataaat ttaccaaact1081 ggaaaaaatt ttcacaaatc atgtcacaaa gagctaattt tcttaaaata tataaaaaag1141 atctttcaaa gtgtcataca caaaacactg cttttcgaaa aacataagct cctacaaagc
1201 agtaagaaaa tgatgaacaa atagaaaaat gggcaaaggt gatgatttaa cagttcacag1261 agaaatacaa atggcttcta aaatatataa aaagatgccc aacttcgttc ataatatgaa1321 aaatgtaaat taagactaca ctaagataca atttttcaca gatctgtttt ctaaaaataa1381 aataagcttg agagagagcc tcttatctga attctgggac agcctgattt gcatgaggga1441 aataggcatc ctcctgcact gctggtggga gggtaaaggt acagtctcag tggaggacag1501 tttgctgtta cctctcagaa ttacaaatgc atgtgtttgt tttgatgtag caatttccca1561 tctaggaatt tattcttgaa agacagtcat acaagatttt cacaccagca ttatttgtaa1621 tagcaaaaag atgggaaatt acctaaatgc tcatatatag gggactgcta aattgtggaa1681 ttccatgcaa ttctaaaaaa aaaaaaaaaa a<210>56<211>1720<212>DNA<213>Homo sapiens<400>561 aatacttttg atcacttcca tttgcctgca aagccagaaa cttctctcaa gaagattata61 gctgagtatg taaaacaaaa tatatgtaca actgtaatac caacaagcac agtggtaagt121 tataacaaga tatacgaagt aatagaaatt aagaagtgag agtatcttca ggcgaaggca181 actgggaagc tggagctcaa agtttaaggc tgtgttaaaa cacaacctaa agttcccagt241 agaagcttgc tatagacttt tggtggctct gagaattccc ctggtcacct cagtgtgagg301 ggtgggtgat tcttaacctc tggtgggttt tgttgcagtt tagctgctgt ggagggattt361 cctacaagga ctggtctcag aacatgtatt tcaactgctc agaagacaac cccagtcgag421 agcgctgctc tgtgccttac tcctgttgct tgcctactcc tgaccaggca gtgatcaaca481 ctatgtgtgg ccaaggtatg caggcctttg actacttgga agctagcaaa gtcatctaca541 ccaatggctg tattgacgag ttggtcaact ggatacacag caacctattc ttacttggtg601 gtgtggctct aggcctggcc atcccccagc tggtaggaat tctgctgtcc cagatcctag661 tgaatcagat caaagatcag atcaagctac agctctacaa ccagcagcac cgggctgacc721 catggtactg agaatccatc ctgcacctcc tcaccatgga aactggcaag cctcataaac781 gaacagcagt gggtgctgaa agcagcacca aatggagatt tggattccag ccccccagtg841 acagcccagt gggaagaagc aaactccaga tgggcagaag gcagggtgca caggtggctc901 cagtctcagg aggatgcgcc tcctctcccc catcccagcc ctcagcattg tgccagagtg961 atacccttaa gtgtttgggt ttatgttttc agttttgttt gggaaacagc agttgcacag1021 agagttgggg gtactgctgc tgccttttca ccgaggcact gccaccacca gctctagcag1081 gggtgctcct gagcttggcg gacatactta gatcctaacg tgccagtgag acctggctgt1141 ggagagtagc actggcagcc ctgcctggac tccacttggc atgataccag ctccagaagg1201 gaagggagtg gagcaggcag tgaggagaga gcctgggggt cggctgggga cagccgtatg1261 tgctaggtag gagtggaggg agatatgttt accaaatgcc tgtcctgcca tcctcccagg1321 tagtcagagt gagctacatc ctgccccacc ttcatttcca tggaaacacg gcagctagga1381 cacggggtac aacagcagcc aaattcttcc ccacctccct tacttcgaaa aaaagtttgg1441 aaccctggtc cctatactct gcagtcagaa gtgggactga gccatacatg cccttgaatt1501 cctccctgtc tggccctccc tctccagcaa gcagggtttt ctttaacttg gcagtgtgca1561 gaggagaagt ggtaacaccc ccaccccatt cccctgcatc ggggctcagt attcctacag1621 ggtaagaggt aggaatcttg ctgggacgag gggagccaga agtggcaata aaagcgtgtt
1681 gacctggaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa<210>57<211>653<212>DNA<213>Homo sapiens<400>571 tttttttttt ttttttgcat acagaattat tttatttaac ttaaaccatg tagtacttta61 ctagaaaaaa gcagagtaag agaaactaac gttgccttag cttcagccat tcaaaataga121 cagtttcttt tttccattat gtaaagaatc cagagtatat cgcaataaca ggaataaatt181 cttacaacag aatatacaaa aacattttga aatttttttc atctactgat tttttatata241 aacaggattt tttaggaata atttatacac agaaagtcat tttatgtaac aaattggcca301 tgttattacc tttttttttc ttacttaaaa aaattttttt ttaacaagaa aactcagaaa361 atgcattatt tgcggtgcat ccattccatc ccgccttctg gtttgatttt ttttatccca421 gacaaaggga tacccagagg tagacaaact ctggcaaacc tctcacctca acctcactgg481 cttaaaaagc agacaggtgt tttcaccggg cgtctcttcc accggtggat gtgtgtgtgc541 aacgccaaac atccaaatga aaagttgaaa acaaaaccca aatagtttcc agatctttct601 cctatgtagc gcaaaaaccc caggtgtttc ccatgataga gatattgtgg agt<210>58<211>1037<212>DNA<213>Homo sapiens<400>581 ttatttchcc gabctccact ctggctccca ccccacaagc gtcagagcag gaaacaagct61 tggstgagat gcctcaggcc tggtaacctg aggtgtagag cacccagaag gaagggtaaa121 agcagggggc aaagcggtgg ccctcccttt ctgggggtca cttctgggct ggggccagct181 gaaacctgtg tccaagtagc tttcagggct ggccacaccc taagccttgc aaaagggcct241 cctgcaaggg ctggcccatg gggtccccac cttcccagcc agtgaggtta gcatggttag301 gagtccacat gtgtgcaagt gcttgtgtgg aggctcatgt atgcatgtgt gtatatgcaa361 agctgcacat gacaatgtgc atgccagtcc agagttagat gtacctatgc agttgccctc421 aagcgaaggg tcatatttgg aaacaaggat ggctctaaac atgtaagcgt gcatgtgggc481 atgtatgtat ctggggccta aggaggtggg gaagtgggtg ttggggtaag ggctggcctt541 cagggcattt gcagaaggag gagtgggtgg gagggaaagg ctgggcagag caggggaagg601 agtgaaagcc aggcaggaaa gtggaagaac aggagaagct catgtaatgg attaccctcc661 acaggattat gttccttgat tcctgagagt tttttctctt gattttaccc cctcagtcta721 tcactgcaag agaaagaggt agaaaagaca aacagaccac aaaagacaag aacccagaca781 tatagacaga cgcacctgtt gcatgtgcat gagccagagc ctgggagaga agagagagcg841 tgcaagagag agctcagagc aggcaggcag cccacccctg cagcagtgct gggctyactg901 gagccctgca gragtcagca gccctgtatg cactcctctg gtttgtcagg taacaggggt961 gcccgccccy tcatgatcag caccggkggt ggggagctgc ttaggtgctc aaryggtctg1021 accatgggkc tggtctc
權(quán)利要求
1.一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列，其特征在于，它包括(a)SEQ ID No.1～SEQ ID No.58所示的序列；(b)SEQ ID No.1～SEQ ID No.58所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.58所示的序列中每條序列有至少70％同源性的序列；(d)上述(a)～(c)中一條或數(shù)條的組合。
2.如權(quán)利要求1所述的表達(dá)序列標(biāo)簽的序列，其特征在于，所述序列包括具有SEQID No.1～SEQ ID No.58所示的序列。
3.一種探針分子，其特征在于，所述的探針分子含有權(quán)利要求1中所述的序列中約8-100個(gè)連續(xù)的核苷酸。
全文摘要
本發(fā)明公開了一類在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽的序列。利用本發(fā)明的在人類肝臟中表達(dá)的表達(dá)序列標(biāo)簽，可以方便的尋找出在人類肝臟中表達(dá)的相關(guān)基因，從而在研究肝臟疾病的致病機(jī)理以及開發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
文檔編號(hào)C12Q1/68GK1955296SQ200510030828
公開日2007年5月2日 申請日期2005年10月28日 優(yōu)先權(quán)日2005年10月28日
發(fā)明者黃健, 韓澤廣 申請人:上海人類基因組研究中心