專利名稱：應(yīng)用于原核生物和真核生物的基于核酸探針的診斷檢驗的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及用于原核生物和/或真核生物的檢測和鑒定的核酸檢驗中靶序列的鑒定。
背景技術(shù)：
ssrA基因編碼小而穩(wěn)定的高拷貝數(shù)的轉(zhuǎn)錄RNA序列(tmRNA)，該基因在所有的細菌中都存在，并且近來證實這種基因還存在于葉綠體和硅藻體中。tmRNA具有tRNA和mRNA分子的雙重功能，并且參與補救失去終止密碼子的截短的mRNA，促進截短的多肽在蛋白酶進行降解之前完成通讀翻譯(Keiler，K.C.et al.(1996)，Science，271，990-993)。tmRNA的這種特殊功能使得研究者開始對其分子的二級結(jié)構(gòu)與功能之間的關(guān)系進行分析。這些研究集中在來自不同微生物的tmRNA的二級結(jié)構(gòu)的保守性以及這種結(jié)構(gòu)保守性在進化上的意義和功能相關(guān)性。Matveeva等(Matveeva.O et al(1998)，Vol.16，No.13，1374-1375)用tmRNA作為含有二級結(jié)構(gòu)的RNA的模型研究了寡核苷酸與RNA分子的結(jié)合。研究沒有實現(xiàn)鑒定tmRNA上的位點以及設(shè)計用于治療目的的反義寡核苷酸的目標。
現(xiàn)在用于細菌診斷的核酸靶點/探針的數(shù)量非常有限。因此，當(dāng)前需要優(yōu)先考慮鑒定出用于診斷目的的DNA和RNA靶點。用于發(fā)展探針的靶核苷酸序列例如可以是質(zhì)粒，核糖體RNA基因，基因之間的序列，編碼毒力因子的基因或隨機基因組DNA片段。此外許多RNA分子已被人描述為可以作為基于RNA的檢測的靶點如核糖體RNA和RNA酶P。
任何基于核酸探針的檢驗都需要對當(dāng)前所研究的真核生物和原核生物的核酸序列的特征有清楚的了解。對原核生物或真核生物進行可靠的檢測，所用核酸探針應(yīng)該為高度特異性(即，和其它生物的核酸序列沒有交叉反應(yīng))和高度敏感性(即，該生物的所有或多數(shù)株系都應(yīng)該與探針反應(yīng)并被檢測到)。所以，優(yōu)選的靶點序列應(yīng)存在于目標生物體的所有的株系之中。該序列應(yīng)具有顯著的序列變異性以保證將目標物種與其它親緣相近的物種能夠區(qū)分開；但另一方面，應(yīng)具有足夠的序列保守性以保證能夠檢測出目標物種的所有株系。一般來說，對一個核酸序列的精確鑒定是進行核酸探針檢驗的基礎(chǔ)，但精確鑒定是費時、困難和不確定的。到目前為止只有為數(shù)不多的幾種方法能夠用于發(fā)展核酸探針來進行較廣范圍的微生物檢測。已經(jīng)被證實可以作為開發(fā)探針中潛在可用的靶點的核酸序列包括如rRNA基因和RNA以及rRNA 16S/23S基因間序列。
多數(shù)核酸探針/靶點檢驗都集中于用細菌的高拷貝核糖體RNA(rRNA)和rRNA 16S/23S間隔區(qū)序列(歐洲專利No.0 395 292)來進行檢測和鑒定。市場上已經(jīng)有的很多成功的商品化細菌檢測試劑盒是基于rRNA探針/靶點來檢測多種微生物。這包括由Genprobe Inc.(SanDiego California)出售的基于16S rRNA的一系列商品化探針診斷試劑盒，以及由Innogenetics N.V.(Ghent，Belgium)出售的基于16S/23S間隔區(qū)序列的DNA探針。但是大多數(shù)這種診斷試劑盒都有其局限性，包括由于靶點序列的低拷貝數(shù)造成的敏感性缺乏以及很多情況下由親緣相近的生物體之間的序列一致性引起的特異性缺乏。
能夠直接應(yīng)用于樣品中指示其中存在的任何活的微生物的基于核酸的檢測方法在食品、工業(yè)、環(huán)境和醫(yī)學(xué)中的應(yīng)用都有重大意義。
基于DNA的檢測方法的一個缺點是他們不能區(qū)分活的和死的生物體。一些研究致力于用rRNA和mRNA作為細胞存活的指示劑(Sheridan，G.E.C.et al.(1998)Applied and Environmental Microbiology，64，1313-1318)。但是這些序列并不是合適的靶點，因為rRNA和mRNA在細菌死后在其細胞中還會存在48小時。
隨著基于核酸的類似微陣列形式的能同時對多核酸靶點進行監(jiān)測的技術(shù)的出現(xiàn)，現(xiàn)在需要鑒別和表征新核酸序列作為探針和/或靶點區(qū)域來檢測和鑒別活的真核細胞和原核細胞。
發(fā)明公開本發(fā)明提供ssrA基因或其片段作為對原核生物或真核生物的核酸探針檢驗中的靶點區(qū)域。
因此，本發(fā)明適用于除病毒外的所有生物體。
尚沒有有關(guān)使用ssrA基因的區(qū)域作為靶點區(qū)來檢測和鑒別原核生物和真核生物的其它核酸探針檢驗及其相應(yīng)的優(yōu)勢的報道。
根據(jù)本發(fā)明的一個實施方案，ssrA基因分子中與從DNA分子5’末端起的高同源性區(qū)相對應(yīng)的片段能夠作為通用靶點區(qū)域。
在本發(fā)明的另一個實施方案中，ssrA基因分子中與從DNA分子3’末端起的高同源性區(qū)相對應(yīng)的片段能夠作為通用靶點區(qū)域。
在本發(fā)明的一個進一步的實施方案中，ssrA基因分子中與低同源性區(qū)域相對應(yīng)的片段能夠作為區(qū)分不同物種的核酸探針檢驗的靶點區(qū)。
在本發(fā)明的另一個進一步的實施方案中，ssrA基因分子中與低同源性區(qū)域相對應(yīng)的片段能夠作為用來產(chǎn)生物屬特異性探針的靶點區(qū)。
如下文所描述，不同生物體的ssrA基因的核酸序列的對比表明這些分子的5’和3’區(qū)具有高度同源性，所以可以用為通用靶點序列區(qū)。在親緣相近的生物體間，ssrA基因比其它細菌高拷貝數(shù)RNA有更顯著程度的序列變異性。這些變異區(qū)可作為區(qū)分不同物種的核酸檢驗的理想靶點。
本發(fā)明還提供了tmRNA即ssrA基因的RNA轉(zhuǎn)錄序列或其片段用作為對原核生物或真核生物的核酸探針檢驗中的靶點區(qū)域。
根據(jù)本發(fā)明這一方面的一個實施方案，tmRNA分子中與從tmRNA5’末端起的一段高同源性區(qū)相對應(yīng)的片段能夠作為通用靶點區(qū)。
可替代地，tmRNA中與從tmRNA 3’末端起的一段高同源性區(qū)相對應(yīng)的片段能夠作為通用靶點區(qū)。
根據(jù)本發(fā)明這一方面的一個進一步實施方案，tmRNA分子中與低同源性區(qū)相對應(yīng)的片段能夠作為區(qū)分不同物種的核酸探針檢驗的靶點區(qū)。
根據(jù)本發(fā)明的另一個進一步的實施方案，tmRNA分子中與低同源性區(qū)域相對應(yīng)的片段能夠作為用來產(chǎn)生屬特異性探針的靶點區(qū)。
根據(jù)本發(fā)明的核酸探針(DNA或RNA)典型地含有來自于ssrA基因或tmRNA轉(zhuǎn)錄序列或它們的互補序列的至少10個核苷酸，這些探針被用于真核生物或原核生物的核酸探針雜交檢驗。探針與它的互補序列的雜交典型地通過在核酸探針上標記上放射性或非放射性(如比色的或熒光的)標記物來揭示。
在優(yōu)選的實施方案中，所述的ssrA基因片段或tmRNA片段可作為用于擴增過程中所用的引物的基礎(chǔ)。
與ssrA基因或tmRNA序列的5’或3’區(qū)相對應(yīng)的通用寡核苷酸引物可按照本發(fā)明使用，以便從廣泛的細菌譜中擴增ssrA基因或它所編碼的tmRNA，有助于實現(xiàn)大范圍生物體的同時擴增，同時能使特異性的核酸探針發(fā)生雜交并進行檢測。
優(yōu)選地，擴增過程的產(chǎn)物可以作為核酸探針檢驗中的靶點區(qū)。
更加優(yōu)選地，tmRNA分子的cDNA轉(zhuǎn)錄序列被作為探針用于核酸雜交檢驗。
這種檢測可以在體外完成或在原位完成。
這里所定義的靶點區(qū)能夠作為區(qū)分活的和死的原核生物或真核生物的檢測的基礎(chǔ)。
與細菌死后存在于其細胞中的rRNA和mRNA不同，tmRNA在死亡的生物體中迅速降解。因此，tmRNA能夠作為有用的靶點來區(qū)分死的和活的原核生物或真核生物，即，通過核酸探針直接與分離的細菌RNA雜交或者通過將RNA擴增并將擴增產(chǎn)物和核酸探針雜交來將死的和活的原核生物或真核生物進行區(qū)分。
優(yōu)選地，靶點區(qū)以多種探針形式使用，以大范圍地檢測和/或鑒定原核生物或真核生物。
根據(jù)本發(fā)明的ssrA基因探針或tmRNA轉(zhuǎn)錄序列探針可以被連接到微陣列基因芯片系統(tǒng)上，以大范圍地對原核生物或真核生物進行高通量的檢測和鑒定。
根據(jù)本發(fā)明的靶點區(qū)能夠作為試驗中的探針來檢測目標樣品中的原核生物或真核生物。
上述目標樣品包括生物樣品例如呼吸道的組織樣品，泌尿生殖道的組織樣品或胃腸道的組織樣品，或者體液如血液和血液組分，唾液或腦脊液。
根據(jù)本發(fā)明的檢驗還可以在其它樣品中完成，如食品樣品，環(huán)境樣品包括空氣、水、海洋和土壤樣品，以及植物和動物來源的樣品。
根據(jù)本發(fā)明，ssrA基因或tmRNA轉(zhuǎn)錄序列的片段可被用于獲得原核生物或真核生物的DNA圖譜的檢驗中，并因此能夠區(qū)分同一物種的不同株系。
核酸序列的對比表明在每一物種之內(nèi)都會在ssrA基因和tmRNA轉(zhuǎn)錄序列內(nèi)發(fā)生序列變異。種內(nèi)序列變異性能被用來區(qū)分同一物種的不同株系來研究流行病學(xué)、追蹤例如爆發(fā)時的感染媒介，或者進行種群研究。
本發(fā)明的其它應(yīng)用包括用ssrA基因，tmRNA轉(zhuǎn)錄序列或與其互補的DNA序列或其片段來設(shè)計用于治療目的的試劑來對抗感染性的原核生物或真核生物。
這些試劑可包括反義mRNA或寡核苷酸、核酶和拮抗性多肽并且可以任何藥學(xué)上適用的形式來使用。
因此，本發(fā)明通過使用tmRNA靶點能被用于只檢測生物樣品中存活的生物體。因此，在任何抗感染藥物治療期間和之后，tmRNA靶點都能夠用于監(jiān)測對于這些特異性感染媒介(如抗微生物和/或抗寄生蟲治療)的治療效率。
在一個實施方案中，靶點區(qū)被用來監(jiān)測藥物對感染媒介的治療效率。
在另一個實施方案中，靶點區(qū)被用來監(jiān)測胃腸道中促進健康的微生物的存活率和水平。
本發(fā)明的這一方面涉及，例如，將包含于如酸乳酪或其它促進健康的食品中的促進健康(益生的)的微生物菌群引入腸道。能夠持續(xù)監(jiān)測這些微生物的存在及水平對于保證它們持續(xù)促進健康的功能是有必要的。tmRNA區(qū)能夠作為檢測存活的微生物，例如在糞便中的微生物的靶點，以監(jiān)測促進健康的生物體的存在。
在一個進一步的實施方案中，檢驗被用于對原核生物或真核生物進行定量分析。
當(dāng)使用探針雜交和/或體外擴增來檢測樣品中的微生物時，可以通過信號強度來測定所存在的微生物的數(shù)量。實時體外擴增的方法能被用來對樣品中的生物體進行定量分析。因此，對樣品中的生物體進行定量分析的能力對用于治療目的的臨床情況是非常重要的，如監(jiān)測抗生素治療或其它治療手段或治療效率。
本發(fā)明的另一個進一步的應(yīng)用是使用ssrA基因序列的數(shù)據(jù)庫來鑒定原核生物或真核生物。
本發(fā)明提供一系列用于ssrA基因和tmRNA序列5’和3’端的同源區(qū)和變異區(qū)的探針，這些探針來自于這些序列或這些序列的互補序列。下面為代表性的序列伴放線菌素放線桿菌(Actinobacillus actinomycetemcomitans)ssrAGGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCGAAGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCCTTTAGCCTTCGCTCCCCAGCTTCCGCTCGTAAGACGGGGATAAAGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCATTAAATTAAATCAAAGTAGCTTAATTGTCGCGTGTCCGTCAGCAGGATTAAGTGAATTTAAAGACCGGACTAAACGTGTAGTGCTAACGGCAGAGGAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO1伴放線菌素放線桿菌(Actinobacillus actinomycetemcomitans)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCGAAGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCCUUUAGCCUUCGCUCCCCAGCUUCCGCUCGUAAGACGGGGAUAAAGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCAUUAAAUUAAAUCAAAGUAGCUUAAUUGUCGCGUGUCCGUCAGCAGGAUUAAGUGAAUUUAAAGACCGGACUAAACGUGUAGUGCUAACGGCAGAGGAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO2殺鮭氣單胞菌(Aeromonas salmonicida)ssrA，內(nèi)部部分AAGATTCACGAAACCCAAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCtTAATAACCTGCATAGAGCCCTTCTACCCTAGCTTGCCTGTGTCCTAGGGAATCGGAAGGTCATCCTTCACAGGATCGTGTGGAAGTCCTGCTCGGGGCGGAAGCATTAAAACCAATCGAGCTAGTCAATTCGTGGCGTGTCTCTCCGCAGCGGGTTGGCGAATGTAAAGAGTGACTAAGCATGTAGTACCGAGGATGTAGTAATTTTGGACGGGG SEQ ID NO3殺鮭氣單胞菌(Aeromonas salmonicida)tmRNA，內(nèi)部部分AAGAUUCACGAAACCCAAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCAUAGAGCCCUUCUACCCUAGCUUGCCUGUGUCCUAGGGAAUCGGAAGGUCAUCCUUCACAGGAUCGUGUGGAAGUCCUGCUCGGGGCGGAAGCAUUAAAACCAAUCGAGCUAGUCAAUUCGUGGCGUGUCUCUCCGCAGCGGGUUGGCGAAUGUAAAGAGUGACUAAGCAUGUAGUACCGAGGAUGUAGUAAUUUUGGACGGGG SEQ ID NO4富養(yǎng)產(chǎn)堿菌(Alcaligenes eutrophus)ssrATGGGCCGACCTGGTTTCGACGTGGTTACAAAGCAGTGAGGCATACCGAGGACCCGTCACCTCGTTAATCAATGGAATGCAATAACTGCTAACGACGAACGTTACGCACTCGCTTAATTGCGGCCGTCCTCGCACTGGCTCGCTGACGGGCTAGGGTCGCAAGACCACGCGAGGTATTTACGTCAGATAAGCTCCGGAAGGGTCACGAAGCCGGGGACGAAAACCTAGTGACTCGCCGTCGTAGAGCGTGTTCGTCCGATGCGCCGGTTAAATCAAATGACAGAACTAAGTATGTAGAACTCTCTGTGGAGGGCTTACGGACGCGGGTTCGATTCCCGCCGGCTCCACCA SEQ IDNO5富養(yǎng)產(chǎn)堿菌(Alcaligenes eutrophus)tmRNAUGGGCCGACCUGGUUUCGACGUGGUUACAAAGCAGUGAGGCAUACCGAGGACCCGUCACCUCGUUAAUCAAUGGAAUGCAAUAACUGCUAACGACGAACGUUACGCACUCGCUUAAUUGCGGCCGUCCUCGCACUGGCUCGCUGACGGGCUAGGGUCGCAAGACCACGCGAGGUAUUUACGUCAGAUAAGCUCCGGAAGGGUCACGAAGCCGGGGACGAAAACCUAGUGACUCGCCGUCGUAGAGCGUGUUCGUCCGAUGCGCCGGUUAAAUCAAAUGACAGAACUAAGUAUGUAGAACUCUCUGUGGAGGGCUUACGGACGCGGGUUCGAUUCCCGCCGGCUCCACCA SEQ ID NO6產(chǎn)液菌(Aquifex aeolicus)ssrAGGGGGCGGAAAGGATTCGACGGGGACAGGCGGTCCCCGAGGAGCAGGCCGGGTGGCTCCCGTAACAGCCGCTAAAACAGCTCCCGAAGCTGAACTCGCTCTCGCTGCCTAATTAAACGGCAGCGCGTCCCCGGTAGGTTTGCGGGTGGCCTACCGGAGGGCGTCAGAGACACCCGCTCGGGCTACTCGGTCGCACGGGGCTGAGTAGCTGACACCTAACCCGTGCTACCCTCGGGGAGCTTGCCCGTGGGCGACCCGAGGGGAAATCCTGAACACGGGCTAAGCCTGTAGAGCCTCGGATGTGGCCGCCGTCCTCGGACGCGGGTTCGATTCCCGCCGCCTCCACCASEQ ID NO7產(chǎn)液菌(Aquifex aeolicus)tmRNAGGGGGCGGAAAGGAUUCGACGGGGACAGGCGGUCCCCGAGGAGCAGGCCGGGUGGCUCCCGUAACAGCCGCUAAAACAGCUCCCGAAGCUGAACUCGCUCUCGCUGCCUAAUUAAACGGCAGCGCGUCCCCGGUAGGUUUGCGGGUGGCCUACCGGAGGGCGUCAGAGACACCCGCUCGGGCUACUCGGUCGCACGGGGCUGAGUAGCUGACACCUAACCCGUGCUACCCUCGGGGAGCUUGCCCGUGGGCGACCCGAGGGGAAAUCCUGAACACGGGCUAAGCCUGUAGAGCCUCGGAUGUGGCCGCCGUCCUCGGACGCGGGUUCGAUUCCCGCCGCCUCCACCA SEQ ID NO8巨大芽孢桿菌(Bacillus megaterium)ssrA，內(nèi)部部分AGGGTAGTTCGAGCTTAGGTTGCGAGTCGAGGAGATGGCCTCGTTAAAACATCAACGCCAATAATAACTGGCAAATCTAACAATAACTTCGCTTTAGCTGCATAATAGTAGCTTAGCGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCACTTTAAGTGGGCTACGCCGGAGTTCGCCGTCTGAGGACGAAGGAAGAGAATAATCAGACTAGCGACTGGGACGCCTGTTGGTAGGCAGAACAGCTCGCGAATGATCAATATGCCAACTACACTCGTAGACGCTTAAGTGGCCATATTTCTGGACGTGG SEQ ID NO9巨大芽孢桿菌(Bacillus megaterium)tmRNA，內(nèi)部部分AGGGUAGUUCGAGCUUAGGUUGCGAGUCGAGGAGAUGGCCUCGUUAAAACAUCAACGCCAAUAAUAACUGGCAAAUCUAACAAUAACUUCGCUUUAGCUGCAUAAUAGUAGCUUAGCGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCACUUUAAGUGGGCUACGCCGGAGUUCGCCGUCUGAGGACGAAGGAAGAGAAUAAUCAGACUAGCGACUGGGACGCCUGUUGGUAGGCAGAACAGCUCGCGAAUGAUCAAUAUGCCAACUACACUCGUAGACGCUUAAGUGGCCAUAUUUCUGGACGUGG SEQ ID NO10枯草芽孢桿菌(Bacillus subtilis)ssrAGGGGACGTTACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAGCTGCCTAATAAGCGCAGCGAGCTCTTCCTGACATTGCCTATGTGTCTGTGAAGAGCACATCCAAGTAGGCTACGCTTGCGTTCCCGTCTGAGAACGTAAGAAGAGATGAACAGACTAGCTCTCGGAAGGCCCGCCCGCAGGCAAGAAGATGAGTGAAACCATAAATATGCAGGCTACGCTCGTAGACGCTTAAGTAATCGATGTTTCTGGACGTGGGTTCGACTCCCACCGTCTCCACCA SEQ ID NO11枯草芽孢桿菌(Bacillus subtilis)tmRNAGGGGACGUUACGGAUUCGACAGGGAUGGAUCGAGCUUGAGCUGCGAGCCGAGAGGCGAUCUCGUAAACACGCACUUAAAUAUAACUGGCAAAACUAACAGUUUUAACCAAAACGUAGCAUUAGCUGCCUAAUAAGCGCAGCGAGCUCUUCCUGACAUUGCCUAUGUGUCUGUGAAGAGCACAUCCAAGUAGGCUACGCUUGCGUUCCCGUCUGAGAACGUAAGAAGAGAUGAACAGACUAGCUCUCGGAAGGCCCGCCCGCAGGCAAGAAGAUGAGUGAAACCAUAAAUAUGCAGGCUACGCUCGUAGACGCUUAAGUAAUCGAUGUUUCUGGACGUGGGUUCGACUCCCACCGUCUCCACCA SEQ ID NO12百日咳鮑特氏菌(Bordetella pertussis)ssrAGGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAGGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTCTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCAGGCGGGGGAAGGCAACTTCACAGGGGGCAACCCCGAACCGCAGCAGCGACATTCACAAGGAATCGGCCACCGCTGGGGTCACACGGCGTTGGTTTAAATTACGTGAATCGCCCTGGTCCGGCCCGTCGATCGGCTAAGTCCAGGGTTAAATCCAAATAGATCGACTAAGCATGTAGAACTGGTTGCGGAGGGCTTGCGGACGGGGGTTCAATTCCCCCCGGCTCCACCASEQ ID NO13百日咳鮑特氏菌(Bordetella pertussis)tmRNAGGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAGGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUCUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCAGGCGGGGGAAGGCAACUUCACAGGGGGCAACCCCGAACCGCAGCAGCGACAUUCACAAGGAAUCGGCCACCGCUGGGGUCACACGGCGUUGGUUUAAAUUACGUGAAUCGCCCUGGUCCGGCCCGUCGAUCGGCUAAGUCCAGGGUUAAAUCCAAAUAGAUCGACUAAGCAUGUAGAACUGGUUGCGGAGGGCUUGCGGACGGGGGUUCAAUUCCCCCCGGCUCCACCASEQ ID NO14布氏疏螺旋體(Borrelia burgdorferi)ssrAGGGGATGTTTTGGATTTGACTGAAAATGTTAATATTGTAAGTTGCAGGCAGAGGGAATCTCTTAAAACTTCTAAAATAAATGCAAAAAATAATAACTTTACAAGCTCAAATCTTGTAATGGCTGCTTAAGTTAGCAGAGGGTTTTGTTGAATTTGGCTTTGAGGTTCACTTATACTCTTTTCGACATCAAAGCTTGCTTAAAAATGTTTTCAAGTTGATTTTTAGGGACTTTTATACTTGAGAGCAATTTGGTGGTTTGCTAGTATTTCCAAACCATATTGCTTAATAAAATACTAGATAAGCTTGTAGAAGCTTATAGTATTATTTTTAGGACGCGGGTTCAATTCCCGCCATCTCCACCA SEQ ID NO15布氏疏螺旋體(Borrelia burgdorferi)tmRNAGGGGAUGUUUUGGAUUUGACUGAAAAUGUUAAUAUUGUAAGUUGCAGGCAGAGGGAAUCUCUUAAAACUUCUAAAAUAAAUGCAAAAAAUAAUAACUUUACAAGCUCAAAUCUUGUAAUGGCUGCUUAAGUUAGCAGAGGGUUUUGUUGAAUUUGGCUUUGAGGUUCACUUAUACUCUUUUCGACAUCAAAGCUUGCUUAAAAAUGUUUUCAAGUUGAUUUUUAGGGACUUUUAUACUUGAGAGCAAUUUGGUGGUUUGCUAGUAUUUCCAAACCAUAUUGCUUAAUAAAAUACUAGAUAAGCUUGUAGAAGCUUAUAGUAUUAUUUUUAGGACGCGGGUUCAAUUCCCGCCAUCUCCACCASEQ ID NO16空腸彎曲桿菌(Campylobacter jejuni)ssrAGGGAGCGACTTGGCTTCGACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGACAGGGGTTCGATTCCCCTCGCTTCCACCASEQ ID NO17空腸彎曲桿菌(Campylobacter jejuni)tmRNAGGGAGCGACUUGGCUUCGACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGGACAGGGGUUCGAUUCCCCUCGCUUCCACCA SEQ ID NO18沙眼衣原體(Chlamydia trachomatis(D/UW-3/CX))ssrAGGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTCGAAGATCTAAGAGTAGCTGCTTAATTAGCAAAGTTGTTACCTAAATACGGGTGACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACACCGTCATGTATCTGGTTAGAACTTAGGTCCTTTAATTCTCGAGGAAATGAGTTTGAAATTTAATGAGAGTCGTTAGTCTCTATAGGGGTTTCTAGCTGAGGAGACATAACGTATAGTACCTAGGAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGACTCTCTCCACCTCCACCA SEQ ID NO19沙眼衣原體(Chlamydia trachomatis(D/UW-3/CX))tmRNAGGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUCGAAGAUCUAAGAGUAGCUGCUUAAUUAGCAAAGUUGUUACCUAAAUACGGGUGACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACACCGUCAUGUAUCUGGUUAGAACUUAGGUCCUUUAAUUCUCGAGGAAAUGAGUUUGAAAUUUAAUGAGAGUCGUUAGUCUCUAUAGGGGUUUCUAGCUGAGGAGACAUAACGUAUAGUACCUAGGAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGACUCUCUCCACCUCCACCASEQ ID NO20沙眼衣原體(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))ssrAGGGGGTGTAAAGGTTTCGACTTAGAAATGAAGCGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCGACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATCAGCTTCGCTGATCTTAATGATCTAAGAGTTGCTGCTTAATTAGCAAAGTTGTTACCTAAGTACTGGTAACCCGGTGTTCGCGAGCTCCACCAGAGGTTTTCGAAACGCCGTCATTTATCTGGTTAGAATTAGGGCCTTTTAACTCTCAAGGGAACTAATTTGAATTTTAATGAGAGTCGTTGGTCTCTATAGAGGTTTCTAGCTGAGGAGATATAACGTAAAATATTCTAGAAACTAAGCATGTAGAGGTTAGCGGGGAGTTTACTAAGGACGAGAGTTCGAATCTCTCCACCTCCACCA SEQ ID NO21沙眼衣原體(鼠肺炎)(Chlamydia trachomatis(mouse pneumonitis))tmRNAGGGGGUGUAAAGGUUUCGACUUAGAAAUGAAGCGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCGACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUCAGCUUCGCUGAUCUUAAUGAUCUAAGAGUUGCUGCUUAAUUAGCAAAGUUGUUACCUAAGUACUGGUAACCCGGUGUUCGCGAGCUCCACCAGAGGUUUUCGAAACGCCGUCAUUUAUCUGGUUAGAAUUAGGGCCUUUUAACUCUCAAGGGAACUAAUUUGAAUUUUAAUGAGAGUCGUUGGUCUCUAUAGAGGUUUCUAGCUGAGGAGAUAUAACGUAAAAAUUCUAGAAACUAAGCAUGUAGAGGUUAGCGGGGAGUUUACUAAGGACGAGAGUUCGAAUCUCUCCACCUCCACCASEQ ID NO22微溫桿狀綠菌(Chlorobium tepidum)ssrAGGGGATGACAGGCTATCGACAGGATAGGTGTGAGATGTCGTTGCACTCCGAGTTTCAGCATGGACGGACTCGTTAAACAAGTCTATGTACCAATAGATGCAGACGATTATTCGTATGCAATGGCTGCCTGATTAGCACAAGTTAATTCAGAAGCCATCGTCCTGCGGTGAATGCGCTTACTCTGAAGCCGCCGGATGGCATAACCCGCGCTTGAGCCTACGGGTTCGCGCAAGTAAGCTCCGTACATTCATGCCCGAGGGGGTGTGCGGGTAACCAATCGGGATAAGGGGACGAACGCTGCTGGCGGTGTAATCGGACCACGAAAAACCAACCACCAGAGATGAGTGTGGTAACTGCATCGAGCAGTGTCCTGGACGCGGGTTCAAGTCCCGCCATCTCCACCA SEQ ID NO23微溫桿狀綠菌(Chlorobium tepidum)tmRNAGGGGAUGACAGGCUAUCGACAGGAUAGGUGUGAGAUGUCGUUGCACUCCGAGUUUCAGCAUGGACGGACUCGUUAAACAAGUCUAUGUACCAAUAGAUGCAGACGAUUAUUCGUAUGCAAUGGCUGCCUGAUUAGCACAAGUUAAUUCAGAAGCCAUCGUCCUGCGGUGAAUGCGCUUACUCUGAAGCCGCCGGAUGGCAUAACCCGCGCUUGAGCCUACGGGUUCGCGCAAGUAAGCUCCGUACAUUCAUGCCCGAGGGGGUGUGCGGGUAACCAAUCGGGAUAAGGGGACGAACGCUGCUGGCGGUGUAAUCGGACCACGAAAAACCAACCACCAGAGAUGAGUGUGGUAACUGCAUCGAGCAGUGUCCUGGACGCGGGUUCAAGUCCCGCCAUCUCCACCA SEQ ID NO24Cyanophora paradoxa(藻類)藍色小體ssrAGGGGCTGTTTAGGTTTCGACGTTTTTTTCTAATTATGTTTGTTAAGCAAGTCGAGGATTTGTTCTATCTCGAAAATCAAGAACTCTCAAAATTTAAACGCAACTAATATTGTACGTTTTAACCGTAAAGCAGCTTTCGCTGTTTAATAATTACTTTTAATTTAAAAACCTAATTTTTTTAGGAATTTATTTATTTATTGTTTATCCTGCTTAATGAATTAAAAAAAGCTATACTTGTGAATAAACGCATAATTTAAAAAAACGGACGTGGGTTCAAATCCCACCAGCTCCACCA SEQ ID NO25Cyanophora paradoxa(藻類)藍色小體tmRNAGGGGCUGUUUAGGUUUCGACGUUUUUUUCUAAUUAUGUUUGUUAAGCAAGUCGAGGAUUUGUUCUAUCUCGAAAAUCAAGAACUCUCAAAAUUUAAACGCAACUAAUAUUGUACGUUUUAACCGUAAAGCAGCUUUCGCUGUUUAAUAAUUACUUUUAAUUUAAAAACCUAAUUUUUUUAGGAAUUUAUUUAUUUAUUGUUUAUCCUGCUUAAUGAAUUAAAAAAAGCUAUACUUGUGAAUAAACGCAUAAUUUAAAAAAACGGACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO26丙酮丁醇梭菌(Clostridium acetobutylicum)ssrA，3′部分AATCTGGCGTCGAGAGCGGGGAAACGAGCCTTACAAAGCTTTGAGTAAGGAACGGAATTTATGAAGCTACTGAAGTGAAAAGCTTGTTTGTAGGCGTTTCATGGAGGGAATGTTAAAATACAAACTGCACTCGGAGATGCTTAATGAAACCATTTTCGGACAGGGGTTCGATTCCCCTCGCCTCCACCA SEQ ID NO27丙酮丁醇梭菌(Clostridium acetobutylicum)tmRNA，3′部分AAUCUGGCGUCGAGAGCGGGGAAACGAGCCUUACAAAGCUUUGAGUAAGGAACGGAAUUUAUGAAGCUACUGAAGUGAAAAGCUUGUUUGUAGGCGUUUCAUGGAGGGAAUGUUAAAAUACAAACUGCACUCGGAGAUGCUUAAUGAAACCAUUUUCGGACAGGGGUUCGAUUCCCCUCGCCUCCACCA SEQ ID NO28放射異常球菌模式種(Deinococcus radiodurans)ssrAGGGGGTGACCCGGTTTCGACAGGGGAACTGAAGGTGATGTTGCGTGTCGAGGTGCCGTTGGCCTCGTAAACAAACGGCAAAGCCATTTAACTGGCAACCAGAACTACGCTCTCGCTGCTTAAGTGAGATGACGACCGTGCAGCCCGGCCTTTGGCGTCGCGGAAGTCACTAAAAAAGAAGGCTAGCCCAGGCGATTCTCCATAGCCGACGGCGAAACTTTATGGAGCTACGGCCTGCGAGAACCTGCCCACTGGTGAGCGCCGGCCCGACAATCAAACAGTGGGATACACACGTAGACGCACGCTGGACGGACCTTTGGACGGCGGTTCGACTCCGCCCACCTCCACCASEQ ID NO29放射異常球菌模式種(Deinococcus radiodurans)tmRNAGGGGGUGACCCGGUUUCGACAGGGGAACUGAAGGUGAUGUUGCGUGUCGAGGUGCCGUUGGCCUCGUAAACAAACGGCAAAGCCAUUUAACUGGCAACCAGAACUACGCUCUCGCUGCUUAAGUGAGAUGACGACCGUGCAGCCCGGCCUUUGGCGUCGCGGAAGUCACUAAAAAAGAAGGCUAGCCCAGGCGAUUCUCCAUAGCCGACGGCGAAACUUUAUGGAGCUACGGCCUGCGAGAACCUGCCCACUGGUGAGCGCCGGCCCGACAAUCAAACAGUGGGAUACACACGUAGACGCACGCUGGACGGACCUUUGGACGGCGGUUCGACUCCGCCCACCUCCACCA SEQ ID NO30脫硫脫硫弧菌模式種(Desulfovibrio desulfuricans)ssrA，內(nèi)部部分GGGACTGGAACCGTAGCGGCAGGTCGAGGCGCCGCTGGCCTCGTAAAAAGCGGCACAAAAGTAATTGCCAACAACGATTACGACTACGCTTACGCTGCCTAATAACAGCGAGGCAATGACCGTTTAACGGTCGCGCCGATCAGGGCCATGCCTGATAACCCTGATTGGCGACACTTATCAGGCTGGCGAAAACCGGCTCTCGCCGGGGTTTTTCGCGAGGAGTTTACCGGCGGGATTGCTGCGTTGTGCCTGGTCAGGGGCCAACAGCGCGGTGAAATACATACTTGACCTAAACCTGTAATGCTTCGTGTGGAATGTTCTCGGACGGGG SEQ ID NO31脫硫脫硫弧菌模式種(Desulfovibrio desulfuricans)tmRNA，內(nèi)部部分GGGACUGGAACCGUAGCGGCAGGUCGAGGCGCCGCUGGCCUCGUAAAAAGCGGCACAAAAGUAAUUGCCAACAACGAUUACGACUACGCUUACGCUGCCUAAUAACAGCGAGGCAAUGACCGUUUAACGGUCGCGCCGAUCAGGGCCAUGCCUGAUAACCCUGAUUGGCGACACUUAUCAGGCUGGCGAAAACCGGCUCUCGCCGGGGUUUUUCGCGAGGAGUUUACCGGCGGGAUUGCUGCGUUGUGCCUGGUCAGGGGCCAACAGCGCGGUGAAAUACAUACUUGACCUAAACCUGUAAUGCUUCGUGUGGAAUGUUCUCGGACGGGG SEQ ID NO32節(jié)瘤腐蹄桿菌(Dichelobacter nodosus)ssrA，3′部分CTCGAGGTGCATGTCGAGAATGAGAGAATCTCGTTAAATACTTTCAAAACTTATAGTTGCAAACGACGACAACTACGCTTTAGCGGCTTAATTCCCGCTTTCGCTTACCTAGATTTGTCTGTGGGTTTACCGTAAGCGACATTAACACAGAATCGCTGGTTAACGCGTCCGCTGTTAATCGGTTAAATTAAGCGGAATCGCTTGTAAAATGCCTGAGCGTTGGCTGTTTATGAGTTAAACCTAATTAACTGCTCTAAACATGTAGTACCAAAAGTTAAGGATTCGCGGACGGGGGTTCAAATCCCCCCGCCTCCACCA SEQ ID NO33節(jié)瘤腐蹄桿菌(Dichelobacter nodosus)tmRNA，3′部分CUCGAGGUGCAUGUCGAGAAUGAGAGAAUCUCGUUAAAUACUUUCAAAACUUAUAGUUGCAAACGACGACAACUACGCUUUAGCGGCUUAAUUCCCGCUUUCGCUUACCUAGAUUUGUCUGUGGGUUUACCGUAAGCGACAUUAACACAGAAUCGCUGGUUAACGCGUCCGCUGUUAAUCGGUUAAAUUAAGCGGAAUCGCUUGUAAAAUGCCUGAGCGUUGGCUGUUUAUGAGUUAAACCUAAUUAACUGCUCUAAACAUGUAGUACCAAAAGUUAAGGAUUCGCGGACGGGGGUUCAAAUCCCCCCGCCUCCACCA SEQ ID NO34腸球菌模式種(Enterococcus faecalis)ssrAGGGGGCGTTACGGATTCGACAGGCATAGTTGAGCTTGAATTGCGTTTCGTAGGTTACGGCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAATTCTTTCGCTTTAGCTGCCTAAAAACCAGCTAGCGAAGATCCTCCCGGCATCGCCCATGTGCTCGGGTCAGGGTCCTAATCGAAGTGGGATACGCTAAATTTTTCCGTCTGTAAAATTTAGAGGAGCTTACCAGACTAGCAATACAGAATGCCTGTCACTCGGCACGCTGTAAAGCGAACCTTTAAATGAGTGTCTATGAACGTAGAGATTTAAGTGGCAATATGTTTGGACGCGGGTTCGACTCCCGCCGTCTCCACCA SEQ ID NO35腸球菌模式種(Enterococcus faecalis)tmRNAGGGGGCGUUACGGAUUCGACAGGCAUAGUUGAGCUUGAAUUGCGUUUCGUAGGUUACGGCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAAUUCUUUCGCUUUAGCUGCCUAAAAACCAGCUAGCGAAGAUCCUCCCGGCAUCGCCCAUGUGCUCGGGUCAGGGUCCUAAUCGAAGUGGGAUACGCUAAAUUUUUCCGUCUGUAAAAUUUAGAGGAGCUUACCAGACUAGCAAUACAGAAUGCCUGUCACUCGGCACGCUGUAAAGCGAACCUUUAAAUGAGUGUCUAUGAACGUAGAGAUUUAAGUGGCAAUAUGUUUGGACGCGGGUUCGACUCCCGCCGUCUCCACCA SEQ ID NO36大腸桿菌(Escherichia coli)ssrAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA SEQ ID NO37大腸桿菌(Escherichia coli)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUUAAUCAGGCUAGUUUGUUAGUGGCGUGUCCGUCCGCAGCUGGCAAGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA SEQ ID NO38流感嗜血桿菌(Haemophilus influenzae)ssrAGGGGCTGATTCTGGATTCGACGGGATTAGCGAAGCCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAAATAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCTCCAGCTTCCGCTCGTAAGACGGGGATAACGCGGAGTCAAACCAAAACGAGATCGTGTGGAAGCCACCGTTTGAGGATCGAAGCACTAAATTGAATCAAACTAGCTTAAGTTTAGCGTGTCTGTCCGCATGCTTAAGTGAAATTAAAGACGAGACTAAACGTGTAGTACTGAAGGTAGAGTAATTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO39流感嗜血桿菌(Haemophilus influenzae)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUAGCGAAGCCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAAAUAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCUCCAGCUUCCGCUCGUAAGACGGGGAUAACGCGGAGUCAAACCAAAACGAGAUCGUGUGGAAGCCACCGUUUGAGGAUCGAAGCACUAAAUUGAAUCAAACUAGCUUAAGUUUAGCGUGUCUGUCCGCAUGCUUAAGUGAAAUUAAAGACGAGACUAAACGUGUAGUACUGAAGGUAGAGUAAUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO40幽門螺桿菌(Helicobacter pylori)(ATCC 43504)ssrA，內(nèi)部部分AGATTTCTTGTCGCGCAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTTAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAACTAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGG SEQ ID NO41幽門螺桿菌(Helicobacter pylori)(ATCC 43504)tmRNA，內(nèi)部部分AGAUUUCUUGUCGCGCAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUUAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAACUAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGSEQ ID NO42幽門螺桿菌(Helicobacter pylori)(菌株26695)ssrAGGGGCTGACTTGGATTTCGACAGATTTCTTGTCGCACAGATAGCATGCCAAGCGCTGCTTGTAAAACAGCAACAAAAATAACTGTAAACAACACAGATTACGCTCCAGCTTACGCTAAAGCTGCGTGAGTTAATCTCCTTTTGGAGCTGGACTGATTAGAATTTCTAGCGTTTTAATCGCTCCATAACCTTAAGCTAGACGCTTTTAAAAGGTGGTTCGCCTTTTAAACTAAGAAACAAGAACTCTTGAAACTATCTCAAGGTTTTAGAAAGTTGGACCAGAGCTAGTTTTAAGGCTAAAAAACCAACCAATTTTCTAAGCATTGTAGAAGTTTGTGTTTAGGGCAAGATTTTTGGACTGGGGTTCGATTCCCCACAGCTCCACCA SEQ ID NO43幽門螺桿菌(Helicobacter pylori)(菌株26695)tmRNAGGGGCUGACUUGGAUUUCGACAGAUUUCUUGUCGCACAGAUAGCAUGCCAAGCGCUGCUUGUAAAACAGCAACAAAAAUAACUGUAAACAACACAGAUUACGCUCCAGCUUACGCUAAAGCUGCGUGAGUUAAUCUCCUUUUGGAGCUGGACUGAUUAGAAUUUCUAGCGUUUUAAUCGCUCCAUAACCUUAAGCUAGACGCUUUUAAAAGGUGGUUCGCCUUUUAAACUAAGAAACAAGAACUCUUGAAACUAUCUCAAGGUUUUAGAAAGUUGGACCAGAGCUAGUUUUAAGGCUAAAAAACCAACCAAUUUUCUAAGCAUUGUAGAAGUUUGUGUUUAGGGCAAGAUUUUUGGACUGGGGUUCGAUUCCCCACAGCUCCACCASEQ ID NO44產(chǎn)氣克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)ssrA，內(nèi)部部分GGGATTCGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTAAGAGCCCTCTCTCCCTAGCTTCCGCTCCTAAGACGGGGAATAAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTAATCAGGCTAGTTTGTCAGTGGCGTGTCCGTCCGCAGCTGGCCAGCGAATGTAAAGACTGGACTAAGCATGTAGTGCCGAGGATGTAGGAATTTC SEQ ID NO45產(chǎn)氣克雷伯氏菌(Klebsiella aerogenes)(NCTC 9528)tmRNA，內(nèi)部部分GGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUAAGAGCCCUCUCUCCCUAGCUUCCGCUCCUAAGACGGGGAAUAAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAAGCCCUGCCUGGGGUUGAAGCGUUAAAACUAAUCAGGCUAGUUUGUCAGUGGCGUGUCCGUCCGCAGCUGGCCAGCGAAUGUAAAGACUGGACUAAGCAUGUAGUGCCGAGGAUGUAGGAAUUUC SEQ ID NO46乳桿菌模式種(Lactobacillus lactis)(NCTC 662)ssrA，內(nèi)部部分AAGCACAGTTCGAGCTTGAATTGCGTTTCGTAGGTTACGTCTACGTTAAAACGTTACAGTTAAATATAACTGCTAAAAACGAAAACAACTCTTACGCTTTAGCTGCCTAAAAACAGTTAGCGTAGATCCTCTCGGCATCGCCCATGTGCTCGAGTAAGGGTCTCAAATTTAGTGGGATACGTTAAACTTTTCCGTCTGTAAAGTTTAAAAGAGATCATCAGACTAGCGATACAGAATGCCTGTCACTCGGCAAGCTGTAAAGCGAAACCTCAAATGAGTTGACTATGAACGTAGATTTTTAAGTGTCGATGTGTTT SEQ ID NO47乳桿菌模式種(Lactobacillus lactis)(NCTC 662)tmRNA，內(nèi)部部分AAGCACAGUUCGAGCUUGAAUUGCGUUUCGUAGGUUACGUCUACGUUAAAACGUUACAGUUAAAUAUAACUGCUAAAAACGAAAACAACUCUUACGCUUUAGCUGCCUAAAAACAGUUAGCGUAGAUCCUCUCGGCAUCGCCCAUGUGCUCGAGUAAGGGUCUCAAAUUUAGUGGGAUACGUUAAACUUUUCCGUCUGUAAAGUUUAAAAGAGAUCAUCAGACUAGCGAUACAGAAUGCCUGUCACUCGGCAAGCUGUAAAGCGAAACCUCAAAUGAGUUGACUAUGAACGUAGAUUUUUAAGUGUCGAUGUGUUU SEQ ID NO48嗜肺軍團菌模式種(Legionella pneumophila)ssrA，內(nèi)部部分GTGGGTTGCAAAACCGGAAGTGCATGCCGAGAAGGAGATCTCTCGTAAATAAGACTCAATTAAATATAAATGCAAACGATGAAAACTTTGCTGGTGGGGAAGCTATCGCTGCCTAATAAGCACTTTAGTTAAACCATCACTGTGTACTGGCCAATAAACCCAGTATCCCGTTCGACCGAGCCCGCTTATCGGTATCGAATCAACGGTCATAAGAGATAAGCTAGCGTCCTAATCTATCCCGGGTTATGGCGCGAAACTCAGGGAATCGCTGTGTATCATCCTGCCCGTCGGAGGAGCCACAGTTAAATTCAAAAGACAAGGCTATGCATGTAGAGCTAAAGGCAGAGGACTTGCGGACGCGG SEQ ID NO49嗜肺軍團菌模式種(Legionella pneumophila)tmRNA，內(nèi)部部分GUGGGUUGCAAAACCGGAAGUGCAUGCCGAGAAGGAGAUCUCUCGUAAAUAAGACUCAAUUAAAUAUAAAUGCAAACGAUGAAAACUUUGCUGGUGGGGAAGCUAUCGCUGCCUAAUAAGCACUUUAGUUAAACCAUCACUGUGUACUGGCCAAUAAACCCAGUAUCCCGUUCGACCGAGCCCGCUUAUCGGUAUCGAAUCAACGGUCAUAAGAGAUAAGCUAGCGUCCUAAUCUAUCCCGGGUUAUGGCGCGAAACUCAGGGAAUCGCUGUGUAUCAUCCUGCCCGUCGGAGGAGCCACAGUUAAAUUCAAAAGACAAGGCUAUGCAUGUAGAGCUAAAGGCAGAGGACUUGCGGACGCGG SEQ ID NO50格氏李斯特氏菌(Listeria grayi)ssrA，內(nèi)部部分ACAGGGATAGGTCGAGCTTGAGTTGCGAGCCGGGGGGATCGGCCCGTCATCAACGTCAAAGCCAATAATAACTGGCAAACAAAACAACAATTTAGCTTTCGCTGCCTAATAGCAGTCTGAATAGCTGATCCTCCGTGCATCACCCATGTGCTACGGTAAGGGTCTCACTTTTAAGTGGGTTACGCTGGCTTATCTCCGTCTGGGGCAAACGAGAAGAGCATAATCAGACTAGCTAGATAGAGCCCTGACGCCGGGCAGACATCTATGCGAAATCCAAATACGGCAACTACGCTCGTAGATGCTCAAGTGCCGATATTTCTGG SEQ ID NO51格氏李斯特氏菌(Listeria grayi)tmRNA，內(nèi)部部分ACAGGGAUAGGUCGAGCUUGAGUUGCGAGCCGGGGGGAUCGGCCCGUCAUCAACGUCAAAGCCAAUAAUAACUGGCAAACAAAACAACAAUUUAGCUUUCGCUGCCUAAUAGCAGUCUGAAUAGCUGAUCCUCCGUGCAUCACCCAUGUGCUACGGUAAGGGUCUCACUUUUAAGUGGGUUACGCUGGCUUAUCUCCGUCUGGGGCAAACGAGAAGAGCAUAAUCAGACUAGCUAGAUAGAGCCCUGACGCCGGGCAGACAUCUAUGCGAAAUCCAAAUACGGCAACUACGCUCGUAGAUGCUCAAGUGCCGAUAUUUCUGG SEQ ID NO52無害李斯特氏菌(Listeria innocua)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG SEQ ID NO53無害李斯特氏菌(Listeria innocua)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG SEQ ID NO54單核細胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 7973)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO55單核細胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC7973)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO56單核細胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)ssrA，內(nèi)部部分CAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTT SEQ ID NO57單核細胞增多性李斯特氏菌(Listeria monocytogenes)(NCTC 11994)tmRNA，內(nèi)部部分CAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUSEQ ID NO58墨氏李斯特氏菌(Listeria murrayi)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATGAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAAGTGCCGATATTTCTGG SEQ ID NO59墨氏李斯特氏菌(Listeria murrayi)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUGAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAAGUGCCGAUAUUUCUGG SEQ ID NO60威氏李斯特氏菌(Listeria welshimeri)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTGGCTAATCTCCGTCTGAGGTTAGTTGGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO61威氏李斯特氏菌(Listeria welshimeri)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUGGCUAAUCUCCGUCUGAGGUUAGUUGGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO62除烴海桿菌(Marinobacter hydrocarbonoclasticus)ssrA，內(nèi)部部分GCCGGTGACGAACCCTTGGGTGCATGCCGAGATGGCAGCGAATCTCGTAAATCCAAAGCTGCAACGTAATAGTCGCAAACGACGAAAACTACGCACTGGCGGCGTAAGCCGTTCCAGTCGTCCTGGCTGAGGCGCCTATAACTCAGTAGCAACATCCCAGGACGTCATCGCTTATAGGCTGCTCCGTTCACCAGAGCTCACTGGTGTTCGGCTAAGATTAAAGAGCTCGCCTCTTGCACCCTGACCTTCGGGTCGCTTGAGGTTAAATCAATAGAAGGACACTAAGCATGTAGACCTCAAGGCCTAGTGCTGGCGGACGCGG SEQ ID NO63除烴海桿菌(Marinobacter hydrocarbonoclasticus)tmRNA，內(nèi)部部分GCCGGUGACGAACCCUUGGGUGCAUGCCGAGAUGGCAGCGAAUCUCGUAAAUCCAAAGCUGCAACGUAAUAGUCGCAAACGACGAAAACUACGCACUGGCGGCGUAAGCCGUUCCAGUCGUCCUGGCUGAGGCGCCUAUAACUCAGUAGCAACAUCCCAGGACGUCAUCGCUUAUAGGCUGCUCCGUUCACCAGAGCUCACUGGUGUUCGGCUAAGAUUAAAGAGCUCGCCUCUUGCACCCUGACCUUCGGGUCGCUUGAGGUUAAAUCAAUAGAAGGACACUAAGCAUGUAGACCUCAAGGCCUAGUGCUGGCGGACGCGG SEQ ID NO64鳥分枝桿菌(Mycobacterium avium)ssrA，內(nèi)部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA SEQ ID NO65鳥分枝桿菌(Mycobacterium avium)tmRNA，內(nèi)部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO66牛分枝桿菌(Mycobacterium bovis)ssrA，內(nèi)部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGG SEQ ID NO67牛分枝桿菌(Mycobacterium bovis)tmRNA，內(nèi)部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGG SEQ ID NO68麻風(fēng)分枝桿菌(Mycobacterium leprae)ssrAGGGGCTGAAAGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAGCAATATAAGCGCCGATTCATATCAGCGCGACTATGCTCTCGCTGCCTAAGCGATGGCTAGTCTGTCAGACCGGGAACGCCCTCGTCCCGGAGCCTGGCATCAGCTAGAGGGATCTACCGATGGGTTCGGTCGCGGGACTCGTCGGGACACCAACCGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGATCAGGAGATCCGAGTAGAGGCATAGCGAACTACGCACGGAGAAGCCTTGAGGGAAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA SEQ ID NO69麻風(fēng)分枝桿菌(Mycobacterium leprae)tmRNAGGGGCUGAAAGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAGCAAUAUAAGCGCCGAUUCAUAUCAGCGCGACUAUGCUCUCGCUGCCUAAGCGAUGGCUAGUCUGUCAGACCGGGAACGCCCUCGUCCCGGAGCCUGGCAUCAGCUAGAGGGAUCUACCGAUGGGUUCGGUCGCGGGACUCGUCGGGACACCAACCGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGAUCAGGAGAUCCGAGUAGAGGCAUAGCGAACUACGCACGGAGAAGCCUUGAGGGAAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA SEQ ID NO70鳥分枝桿菌(副結(jié)核亞種)(Mycobacterium paratuberculosis)ssrA，內(nèi)部部分TTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACTGACCACCGTAAGCGTCGTTGCAAATAGATAAGCGCCGATTCACATCAGCGCGACTTACCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGCCCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTTGTTCGCGTGACCAGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGAACCCGGGTTCGATTCCCAA SEQ ID NO71鳥分枝桿菌(副結(jié)核亞種)(Mycobacterium paratuberculosis)tmRNA，內(nèi)部部分UUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACUGACCACCGUAAGCGUCGUUGCAAAUAGAUAAGCGCCGAUUCACAUCAGCGCGACUUACCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGCCCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUUGUUCGCGUGACCAGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGAACCCGGGUUCGAUUCCCAASEQ ID NO72結(jié)核分枝桿菌(Mycobacterium tuberculosis)ssrAGGGGCTGAACGGTTTCGACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAGGACCCGGGTTCGATTCCCGGCAGCTCCACCA SEQ ID NO73結(jié)核分枝桿菌(Mycobacterium tuberculosis)tmRNAGGGGCUGAACGGUUUCGACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAGGACCCGGGUUCGAUUCCCGGCAGCUCCACCA SEQ ID NO74山羊枝原體(Mycoplasma capricolum)ssrAGGGGATGTCATGGATTTGACAGGATATCTTTAGTACATATAAGCAGTAGTGTTGTAGACTATAAATACTACTAGGTTTAAAAAAACGCAAATAAAAACGAAGAAACTTTTGAAATGCCAGCATTTATGATGAATAATGCATCAGCTGGAGCAAACTTTATGTTTGCTTAATAACTACTAGTTTAGTTATAGTATTTCACGAATTATAGATATTTTAAGCTTTATTTATAACCGTATTACCCAAGCTTAATAGAATATATGATTGCAATAAATATATTTGAAATCTAATTGCAAATGATATTTAACCTTTAGTTAATTTTAGTTAAATATTTTAATTAGAAAATTAACTAAACTGTAGAAAGTATGTATTAATATATCTTGGACGCGAGTTCGATTCTCGCCATCTCCACCA SEQ ID NO75山羊枝原體(Mycoplasma capricolum)tmRNAGGGGAUGUCAUGGAUUUGACAGGAUAUCUUUAGUACAUAUAAGCAGUAGUGUUGUAGACUAUAAAUACUACUAGGUUUAAAAAAACGCAAAUAAAAACGAAGAAACUUUUGAAAUGCCAGCAUUUAUGAUGAAUAAUGCAUCAGCUGGAGCAAACUUUAUGUUUGCUUAAUAACUACUAGUUUAGUUAUAGUAUUUCACGAAUUAUAGAUAUUUUAAGCUUUAUUUAUAACCGUAUUACCCAAGCUUAAUAGAAUAUAUGAUUGCAAUAAAUAUAUUUGAAAUCUAAUUGCAAAUGAUAUUUAACCUUUAGUUAAUUUUAGUUAAAUAUUUUAAUUAGAAAAUUAACUAAACUGUAGAAAGUAUGUAUUAAUAUAUCUUGGACGCGAGUUCGAUUCUCGCCAUCUCCACCA SEQ ID NO76生殖道枝原體(Mycoplasma genitalium)(ATTC 33530，#1)ssrAGGGGATGTTTTGGGTTTGACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGAACCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA SEQ ID NO77生殖道枝原體(Mycoplasma genitalium)(ATTC 33530，#1)tmRNAGGGGAUGUUUUGGGUUUGACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAACCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA SEQ ID NO78生殖道枝原體(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，內(nèi)部部分ACATAATGCTGATAGACAAACAGTAGCATTGGGGTATGCCCCTTACAGCGCTAGGTTCAATAACCGACAAAGAAAATAACGAAGTGTTGGTAGATCCAAATTTGATCATTAACCAACAAGCAAGTGTTAACTTTGCTTTTGCATAAGTAGATACTAAAGCTACAGCTGGTGAATAGTCATAGTTTGCTAGCTGTCATAGTTTATGACTCGAGGTTAAATCGTTCAATTTAACCTTTAAAAATAGAACTTGTTGTTTCCATGATTGTTTTGTGATCAATTGGAAACAAGACAAAAATCCACAAAACTAAAATGTAGAAGCTGTTTGTTGTGTCCTTTATGGAAACGGGTTCSEQ ID NO79生殖道枝原體(Mycoplasma genitalium)(ATTC 33530，#2)tmRNA，內(nèi)部部分ACAUAAUGCUGAUAGACAAACAGUAGCAUUGGGGUAUGCCCCUUACAGCGCUAGGUUCAAUAACCGACAAAGAAAAUAACGAAGUGUUGGUAGAUCCAAAUUUGAUCAUUAACCAACAAGCAAGUGUUAACUUUGCUUUUGCAUAAGUAGAUACUAAAGCUACAGCUGGUGAAUAGUCAUAGUUUGCUAGCUGUCAUAGUUUAUGACUCGAGGUUAAAUCGUUCAAUUUAACCUUUAAAAAUAGAACUUGUUGUUUCCAUGAUUGUUUUGUGAUCAAUUGGAAACAAGACAAAAAUCCACAAAACUAAAAUGUAGAAGCUGUUUGUUGUGUCCUUUAUGGAAACGGGUUC SEQ ID NO80嗜肺枝原體(Mycoplasma pneumophila)ssrAGGGGATGTAGAGGTTTTGACATAATGTTGAAAGGAAAACAGTTGCAGTGGGGTATGCCCCTTACAGCTCTAGGTATAATAACCGACAAAAATAACGACGAAGTTTTGGTAGATCCAATGTTGATCGCTAACCAACAAGCAAGTATCAACTACGCTTTCGCTTAGAACATACTAAAGCTACACGAATTGAATCGCCATAGTTTGGTTCGTGTCACAGTTTATGGCTCGGGGTTAACTGGTTCAACTTAATCCTTAAATTATGAACTTATCGTTTACTTGTTTGTCTTATGATCTAAAGTAAGCGAGACATTAAAACATAAGACTAAACTGTAGAAGCTGTTTTACCAATCCTTTATGGAAACGGGTTCGATTCCCGTCATCTCCACCA SEQ ID NO81嗜肺枝原體(Mycoplasma pneumophila)tmRNAGGGGAUGUAGAGGUUUUGACAUAAUGUUGAAAGGAAAACAGUUGCAGUGGGGUAUGCCCCUUACAGCUCUAGGUAUAAUAACCGACAAAAAUAACGACGAAGUUUUGGUAGAUCCAAUGUUGAUCGCUAACCAACAAGCAAGUAUCAACUACGCUUUCGCUUAGAACAUACUAAAGCUACACGAAUUGAAUCGCCAUAGUUUGGUUCGUGUCACAGUUUAUGGCUCGGGGUUAACUGGUUCAACUUAAUCCUUAAAUUAUGAACUUAUCGUUUACUUGUUUGUCUUAUGAUCUAAAGUAAGCGAGACAUUAAAACAUAAGACUAAACUGUAGAAGCUGUUUUACCAAUCCUUUAUGGAAACGGGUUCGAUUCCCGUCAUCUCCACCA SEQ ID NO82淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)ssrA，內(nèi)部部分GGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGG SEQ ID NO83淋病奈瑟球菌(Neisseria gonorrhoeae)(ATCC 19424)tmRNA，內(nèi)部部分GGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGG SEQ ID NO84淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)ssrAGGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGTGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCAGCCGGGTTACTTGGCAGGAAATAAGACTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA SEQ ID NO85淋病奈瑟球菌(Neisseria gonorrhoeae)(FA 1090)tmRNAGGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGUGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCAGCCGGGUUACUUGGCAGGAAAUAAGACUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA SEQ ID NO86腦膜炎奈瑟氏球菌(Neisseria meningitidis)ssrAGGGGGCGACCTTGGTTTCGACGGGGGTTGCGAAGCAGATGCGGGCATACCGGGGTCTCAGATTCCCGTAAAACACTGAATTCAAATAGTCGCAAACGACGAAACTTACGCTTTAGCCGCTTAAGGCTAGCCGTTGCAGCAGTCGGTCAATGGGCTGTGTGGCGAAAGCCACCGCAACGTCATCTTACATTGACTGGTTTCCTGCCGGGTTATTTGGCAGGAAATGAGATTTAAGGTAACTGGTTTCCAAAAGGCCTGTTGGTCGGCATGATGGAAATAAGATTTTCAAATAGACACAACTAAGTATGTAGAACGCTTTGTAGAGGACTTTCGGACGGGGGTTCGATTCCCCCCGCCTCCACCA SEQ ID NO87腦膜炎奈瑟氏球菌(Neisseria meningitidis)tmRNAGGGGGCGACCUUGGUUUCGACGGGGGUUGCGAAGCAGAUGCGGGCAUACCGGGGUCUCAGAUUCCCGUAAAACACUGAAUUCAAAUAGUCGCAAACGACGAAACUUACGCUUUAGCCGCUUAAGGCUAGCCGUUGCAGCAGUCGGUCAAUGGGCUGUGUGGCGAAAGCCACCGCAACGUCAUCUUACAUUGACUGGUUUCCUGCCGGGUUAUUUGGCAGGAAAUGAGAUUUAAGGUAACUGGUUUCCAAAAGGCCUGUUGGUCGGCAUGAUGGAAAUAAGAUUUUCAAAUAGACACAACUAAGUAUGUAGAACGCUUUGUAGAGGACUUUCGGACGGGGGUUCGAUUCCCCCCGCCUCCACCA SEQ ID NO88Nostoc muscorum PCC7120 ssrAGGGTCCGTCGGTTTCGACAGGTTGGCGAACGCTACTCTGTGATTCAGGTCGAGAGTGAGTCTCCTCTGCAAATCAAGGCTCAAAACAAAAGTAAATGCGAATAACATCGTTAAATTTGCTCGTAAGGACGCTCTAGTAGCTGCCTAAATAGCCTCTTTCAGGTTCGAGCGTCTTCGGTTTGACTCCGTTAAGGACTGAAGACCAACCCCCAACGGATGCTCTAGCAATGTTCTCTGGTTGGCTTGCTAGCTAAGATTTAATCAGAGCATCCTACGTTCGGGATAATGAACGATTCCCGCCTTGAGGGTCAGAAAGGCTAAACCTGTGAATGAGCGGGGGGTCAATACCCAATTTGGACAGCAGTTCGACTCTGCTCGATCCACCA SEQ ID NO89Nostoc muscorum PCC7120 tmRNAGGGUCCGUCGGUUUCGACAGGUUGGCGAACGCUACUCUGUGAUUCAGGUCGAGAGUGAGUCUCCUCUGCAAAUCAAGGCUCAAAACAAAAGUAAAUGCGAAUAACAUCGUUAAAUUUGCUCGUAAGGACGCUCUAGUAGCUGCCUAAAUAGCCUCUUUCAGGUUCGAGCGUCUUCGGUUUGACUCCGUUAAGGACUGAAGACCAACCCCCAACGGAUGCUCUAGCAAUGUUCUCUGGUUGGCUUGCUAGCUAAGAUUUAAUCAGAGCAUCCUACGUUCGGGAUAAUGAACGAUUCCCGCCUUGAGGGUCAGAAAGGCUAAACCUGUGAAUGAGCGGGGGGUCAAUACCCAAUUUGGACAGCAGUUCGACUCUGCUCGAUCCACCASEQ ID NO90Odontella sinensis(硅藻屬)葉綠體ssrAGGGGCTGACTTGGTTTCGACATTTAAAAATTGTTACAGTATGATGCAGGTCGAAGTTTCTAATCTTCGTAAAAAAAGAGAAATTTATAATAAATGCTAATAATTTAATTTCTTCTGTGTTTAAAAGTTTATCAACTAAGCAAAATAGTTTAAATTTAAGTTTTGCTGTTTAAGTTTTATGCACATTTAATGATCTAGTAAATAACTTTGTTCGCTATAATTTATATTTATAACTAGACTTTTGTCTTTTTTATAGTTTAGAATAACTTTATCATTTCAAACCTCGTTCCATCTAGTTGAACTAAACCTGTGAACGAATACTATAATAAAATTTTTAGATGGACGTGGGTTCGACTCCCATCAGCTCCACCA SEQ ID NO91Odontella sinensis(硅藻屬)葉綠體tmRNAGGGGCUGACUUGGUUUCGACAUUUAAAAAUUGUUACAGUAUGAUGCAGGUCGAAGUUUCUAAUCUUCGUAAAAAAAGAGAAAUUUAUAAUAAAUGCUAAUAAUUUAAUUUCUUCUGUGUUUAAAAGUUUAUCAACUAAGCAAAAUAGUUUAAAUUUAAGUUUUGCUGUUUAAGUUUUAUGCACAUUUAAUGAUCUAGUAAAUAACUUUGUUCGCUAUAAUUUAUAUUUAUAACUAGACUUUUGUCUUUUUUAUAGUUUAGAAUAACUUUAUCAUUUCAAACCUCGUUCCAUCUAGUUGAACUAAACCUGUGAACGAAUACUAUAAUAAAAUUUUUAGAUGGACGUGGGUUCGACUCCCAUCAGCUCCACCASEQ ID NO92卟啉單胞菌(Porphyra purpureum)(紅藻)葉綠體ssrAGGGGCTGCAAGGTTTCTACATTGTGAAAAAACAAATATATGAAAGTAAAACGAGCTCATTATTAGAGCTTTTAGTTAAATAAATGCAGAAAATAATATTATTGCTTTTTCTCGAAAATTAGCTGTTGCATAAATAGTCTCAATTTTTGTAATTCGAAGTGATAGACTCTTATACACTACGAATATTCTGTTAGAGTTGCTCTTAATAAAAGAAAAGTAAAAAAATACAAATTCTTATGTTTTTTACCTGAATTGATTCAATTTAAGGTTAGTATTTTTTGATTTTTACAATGGACGTGGGTTCAAGTCCCACCAGCTCCACCA SEQ ID NO93卟啉單胞菌(Porphyra purpureum)(紅藻)葉綠體tmRNAGGGGCUGCAAGGUUUCUACAUUGUGAAAAAACAAAUAUAUGAAAGUAAAACGAGCUCAUUAUUAGAGCUUUUAGUUAAAUAAAUGCAGAAAAUAAUAUUAUUGCUUUUUCUCGAAAAUUAGCUGUUGCAUAAAUAGUCUCAAUUUUUGUAAUUCGAAGUGAUAGACUCUUAUACACUACGAAUAUUCUGUUAGAGUUGCUCUUAAUAAAAGAAAAGUAAAAAAAUACAAAUUCUUAUGUUUUUUACCUGAAUUGAUUCAAUUUAAGGUUAGUAUUUUUUGAUUUUUACAAUGGACGUGGGUUCAAGUCCCACCAGCUCCACCA SEQ ID NO94牙齦卟啉單胞菌(Porphyromonas gingivalis)ssrAGGGGCTGACCGGCTTTGACAGCGTGATGAAGCGGTATGTAAGCATGTAGTGCGTGGGTGGCTTGCACTATAATCTCAGACATCAAAAGTTTAATTGGCGAAAATAACTACGCTCTCGCTGCGTAATCGAAGAATAGTAGATTAGACGCTTCATCGCCGCCAAAGTGGCAGCGACGAGACATCGCCCGAGCAGCTTTTTCCCGAAGTAGCTCGATGGTGCGGTGCTGACAAATCGGGAACCGCTACAGGATGCTTCCTGCCTGTGGTCAGATCGAACGGAAGATAAGGATCGTGCATTGGGTCGTTTCAGCCTCCGCTCGCTCACGAAAATTCCAACTGAAACTAAACATGTAGAAAGCATATTGATTCCATGTTTGGACGAGGGTTCAATTCCCTCCAGCTCCACCA SEQ ID NO95牙齦卟啉單胞菌(Porphyromonas gingivalis)tmRNAGGGGCUGACCGGCUUUGACAGCGUGAUGAAGCGGUAUGUAAGCAUGUAGUGCGUGGGUGGCUUGCACUAUAAUCUCAGACAUCAAAAGUUUAAUUGGCGAAAAUAACUACGCUCUCGCUGCGUAAUCGAAGAAUAGUAGAUUAGACGCUUCAUCGCCGCCAAAGUGGCAGCGACGAGACAUCGCCCGAGCAGCUUUUUCCCGAAGUAGCUCGAUGGUGCGGUGCUGACAAAUCGGGAACCGCUACAGGAUGCUUCCUGCCUGUGGUCAGAUCGAACGGAAGAUAAGGAUCGUGCAUUGGGUCGUUUCAGCCUCCGCUCGCUCACGAAAAUUCCAACUGAAACUAAACAUGUAGAAAGCAUAUUGAUUCCAUGUUUGGACGAGGGUUCAAUUCCCUCCAGCUCCACCA SEQ ID NO96雷氏變性菌(Proteus rettgeri)ssrA(NCTC 10975)，內(nèi)部部分GGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCTTGCCTGGGGTTGAAGCGTTAAACTTAATCAGGATAGTTTGTTGGTGGCGTGTCTGTCCGCAGCTGGCAAATGAATTCAAAGACTAGACTAAGCATGTAGTACCGAGGATGTAGAAATTTCSEQ ID NO97雷氏變性菌(Proteus rettgeri)ssrA tmRNA(NCTC 10975)，內(nèi)部部分GGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCUUGCCUGGGGUUGAAGCGUUAAACUUAAUCAGGAUAGUUUGUUGGUGGCGUGUCUGUCCGCAGCUGGCAAAUGAAUUCAAAGACUAGACUAAGCAUGUAGUACCGAGGAUGUAGAAAUUUC SEQ ID NO98游海假交替單胞菌(Pseudoalteromonas haloplanktoni)ssrA，內(nèi)部部分GGAATTCAAGAAGCCCGAGGTGCATGTCGAGGTGCGGTTTGCCTCGTAAAAAAGCCGCAATTTAAAGTAATCGCAAACGACGATAACTACTCTCTAGCAGCTTAGGCTGGCTAGCGCTCCTTCCATGTATTCTTGTGGACTGGATTTTGGAGTGTCACCCTAACACCTGATCGCGACGGAAACCCTGGCCGGGGTTGAAGCGTTAAAACTAAGCGGCCTCGCCTTTATCTACCGTGTTTGTCCGGGATTTAAAGGTTAATTAAATGACAATACTAAACATGTAGTACCGACGGTCGAGGCTTTTCGGACGGGG SEQ ID NO99游海假交替單胞菌(Pseudoalteromonas haloplanktoni)tmRNA，內(nèi)部部分GGAAUUCAAGAAGCCCGAGGUGCAUGUCGAGGUGCGGUUUGCCUCGUAAAAAAGCCGCAAUUUAAAGUAAUCGCAAACGACGAUAACUACUCUCUAGCAGCUUAGGCUGGCUAGCGCUCCUUCCAUGUAUUCUUGUGGACUGGAUUUUGGAGUGUCACCCUAACACCUGAUCGCGACGGAAACCCUGGCCGGGGUUGAAGCGUUAAAACUAAGCGGCCUCGCCUUUAUCUACCGUGUUUGUCCGGGAUUUAAAGGUUAAUUAAAUGACAAUACUAAACAUGUAGUACCGACGGUCGAGGCUUUUCGGACGGGG SEQ ID NO100銅綠假單胞菌(Pseudomonas aeruginosa)ssrAGGGGCCGATTAGGATTCGACGCCGGTAACAAAAGTTGAGGGGCATGCCGAGTTGGTAGCAGAACTCGTAAATTCGCTGCTGCAAACTTATAGTTGCCAACGACGACAACTACGCTCTAGCTGCTTAATGCGGCTAGCAGTCGCTAGGGGATGCCTGTAAACCCGAAACGACTGTCAGATAGAACAGGATCGCCGCCAAGTTCGCTGTAGACGTAACGGCTAAAACTCATACAGCTCGCTCCAAGCACCCTGCCACTCGGGCGGCGCGGAGTTAACTCAGTAGAGCTGGCTAAGCATGTAAAACCGATAGCGGAAAGCTGGCGGACGGGGGTTCAAATCCCCCCGGTTCCACCASEQ ID NO101銅綠假單胞菌(Pseudomonas aeruginosa)tmRNAGGGGCCGAUUAGGAUUCGACGCCGGUAACAAAAGUUGAGGGGCAUGCCGAGUUGGUAGCAGAACUCGUAAAUUCGCUGCUGCAAACUUAUAGUUGCCAACGACGACAACUACGCUCUAGCUGCUUAAUGCGGCUAGCAGUCGCUAGGGGAUGCCUGUAAACCCGAAACGACUGUCAGAUAGAACAGGAUCGCCGCCAAGUUCGCUGUAGACGUAACGGCUAAAACUCAUACAGCUCGCUCCAAGCACCCUGCCACUCGGGCGGCGCGGAGUUAACUCAGUAGAGCUGGCUAAGCAUGUAAAACCGAUAGCGGAAAGCUGGCGGACGGGGGUUCAAAUCCCCCCGGUUCCACCA SEQ ID NO102鼠傷寒沙門氏菌(Salmonella typhimurium)ssrAGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGCGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCA SEQ ID NO103鼠傷寒沙門氏菌(Salmonella typhimurium)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGCGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGGACGCGGGUUCAACUCCCGCCAGCUCCACCA SEQ ID NO104希瓦氏菌模式種(Shewanella putrefaciens)ssrAGGGGGCGATTCTGGATTCGACAGGATTCACGAAACCCTGGGAGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAGTTATAGTTGCAAACGACGATAACTACGCTCTAGCCGCTTAATGCCGCTAGCCATCTACCACACGCTTTGCACATGGGCAGTGGATTTGATGGTCATCTCACATCGTGCTAGCGAGGGAACCCTGTCTGGGGGTGAACCGCGAAACAGTACCGGACTCACCGTGTGGGATCCTGTCTTTCGGAGTTCAAACGGTTAAACAATAGAAAGACTAAGCATGTAGCGCCTTGGATGTAGGTTTTCTGGACGCGGGTTCAAGTCCCGCCGCCTCCACCASEQ ID NO105希瓦氏菌模式種(Shewanella putrefaciens)tmRNAGGGGGCGAUUCUGGAUUCGACAGGAUUCACGAAACCCUGGGAGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAGUUAUAGUUGCAAACGACGAUAACUACGCUCUAGCCGCUUAAUGCCGCUAGCCAUCUACCACACGCUUUGCACAUGGGCAGUGGAUUUGAUGGUCAUCUCACAUCGUGCUAGCGAGGGAACCCUGUCUGGGGGUGAACCGCGAAACAGUACCGGACUCACCGUGUGGGAUCCUGUCUUUCGGAGUUCAAACGGUUAAACAAUAGAAAGACUAAGCAUGUAGCGCCUUGGAUGUAGGUUUUCUGGACGCGGGUUCAAGUCCCGCCGCCUCCACCA SEQ ID NO106金黃色葡萄球菌(Staphylococcus aureus)ssrAGGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTCGGAGGGTTGTCTTCGTCATCAACACACACAGTTTATAATAACTGGCAAATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAGCATTTCCTATGTGCTGTTAACGCGATTCAACCTTAATAGGATATGCTAAACACTGCCGTTTGAAGTCTGTTTAGAAGAAACTTAATCAAACTAGCATCATGTTGGTTGTTTATCACTTTTCATGATGCGAAACCTATCGATAAACTACACACGTAGAAAGATGTGTATCAGGACCTTTGGACGCGGGTTCAAATCCCGCCGTCTCCACCA SEQ ID NO107金黃色葡萄球菌(Staphylococcus aureus)tmRNAGGGGACGUUCAUGGAUUCGACAGGGGUCCCCCGAGCUCAUUAAGCGUGUCGGAGGGUUGUCUUCGUCAUCAACACACACAGUUUAUAAUAACUGGCAAAUCAAACAAUAAUUUCGCAGUAGCUGCCUAAUCGCACUCUGCAUCGCCUAACAGCAUUUCCUAUGUGCUGUUAACGCGAUUCAACCUUAAUAGGAUAUGCUAAACACUGCCGUUUGAAGUCUGUUUAGAAGAAACUUAAUCAAACUAGCAUCAUGUUGGUUGUUUAUCACUUUUCAUGAUGCGAAACCUAUCGAUAAACUACACACGUAGAAAGAUGUGUAUCAGGACCUUUGGACGCGGGUUCAAAUCCCGCCGUCUCCACCA SEQ ID NO108戈氏鏈球菌(Streptococcus gordonii)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATAATACTTCTTACGCTTTAGCTGCCTAAAAACCAGCGGGCGTGACCCGATTCGGATTGCTTGTGTCTGATGACAGGTCTTATTATTAGCAAGCTACGGTAGAATCTTGTCTAGTGATTTTACAAGAGATTGATAGACTCGCTTGATTTGGGCTTGAGTTATGTGTCAAAATCAAGTTAAAACAATACATAGCCTATGGTTGTAGACAAATGTGTTGGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCA SEQ ID NO109戈氏鏈球菌(Streptococcus gordonii)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUAAUACUUCUUACGCUUUAGCUGCCUAAAAACCAGCGGGCGUGACCCGAUUCGGAUUGCUUGUGUCUGAUGACAGGUCUUAUUAUUAGCAAGCUACGGUAGAAUCUUGUCUAGUGAUUUUACAAGAGAUUGAUAGACUCGCUUGAUUUGGGCUUGAGUUAUGUGUCAAAAUCAAGUUAAAACAAUACAUAGCCUAUGGUUGUAGACAAAUGUGUUGGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO110變異鏈球菌(Streptococcus mutans)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGACCTATTTTGCGACTCATCTAGCGGATGTAAAACGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCAGTAGCTGCCTAAAAACCAGCCTGTGTGATCAATAACAAATTGCTTGTGTTTGTTGATTGGTCTTATTGTTAACAAGCTACGTTAGAACTGAGTCAGGCTGTTCTAAAAGAGTTCTACTGACTCGCATCGTTAGAGTTTGAGTTATGTATTGTAACGGTGTTAAATAAACACATAACCTATAGTTGTAGACAAATGGGTTAGCAGATGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO111變異鏈球菌(Streptococcus mutans)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGACCUAUUUUGCGACUCAUCUAGCGGAUGUAAAACGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAGUAGCUGCCUAAAAACCAGCCUGUGUGAUCAAUAACAAAUUGCUUGUGUUUGUUGAUUGGUCUUAUUGUUAACAAGCUACGUUAGAACUGAGUCAGGCUGUUCUAAAAGAGUUCUACUGACUCGCAUCGUUAGAGUUUGAGUUAUGUAUUGUAACGGUGUUAAAUAAACACAUAACCUAUAGUUGUAGACAAAUGGGUUAGCAGAUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO112肺炎鏈球菌(Streptococcus pneumoniae)ssrAGGGGTCGTTACGGATTCGACAGGCATTATGAGGCATATTTTGCGACTCGTGTGGCGACGTAAACGCTCAGTTAAATATAACTGCAAAAAATAACACTTCTTACGCTCTAGCTGCCTAAAAACCAGCAGGCGTGACCCGATTTGGATTGCTCGTGTTCAATGACAGGTCTTATTATTAGCGAGATACGATTAAGCCTTGTCTAGCGGTTTGATAAGAGATTGATAGACTCGCAGTTTCTAGACTTGAGTTATGTGTCGAGGGGCTGTTAAAATAATACATAACCTATGGTTGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCGGCTCCACCASEQ ID NO113肺炎鏈球菌(Streptococcus pneumoniae)tmRNAGGGGUCGUUACGGAUUCGACAGGCAUUAUGAGGCAUAUUUUGCGACUCGUGUGGCGACGUAAACGCUCAGUUAAAUAUAACUGCAAAAAAUAACACUUCUUACGCUCUAGCUGCCUAAAAACCAGCAGGCGUGACCCGAUUUGGAUUGCUCGUGUUCAAUGACAGGUCUUAUUAUUAGCGAGAUACGAUUAAGCCUUGUCUAGCGGUUUGAUAAGAGAUUGAUAGACUCGCAGUUUCUAGACUUGAGUUAUGUGUCGAGGGGCUGUUAAAAUAAUACAUAACCUAUGGUUGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCGGCUCCACCA SEQ ID NO114化膿鏈球菌(Streptococcus pyogenes)ssrAGGGGTTGTTACGGATTCGACAGGCATTATGAGGCATGTTTTGCGTCCCATCGGCAGATGTAAATTGCCAGTTAAATATAACTGCAAAAAATACAAACTCTTACGCTTTAGCTGCCTAAAAACCAGCTAGCGTGACTTCTACAAGATTGCTTGTGTCCTGTTAGAAGTCTCAAAATAGCAAGCTACGGTTACGAAATTGTCTAGTTTCGTGACAAGAGATTGATAGACTCGCAAACTAATGGCTTGAGTTATGTGTCTTTAGTTTGTTAAATGAAGACATAACCTATGGACGTAGACAAATATGTTGGCAGGTGTTTGGACGTGGGTTCGACTCCCACCAGCTCCACCASEQ ID NO115化膿鏈球菌(Streptococcus pyogenes)tmRNAGGGGUUGUUACGGAUUCGACAGGCAUUAUGAGGCAUGUUUUGCGUCCCAUCGGCAGAUGUAAAUUGCCAGUUAAAUAUAACUGCAAAAAAUACAAACUCUUACGCUUUAGCUGCCUAAAAACCAGCUAGCGUGACUUCUACAAGAUUGCUUGUGUCCUGUUAGAAGUCUCAAAAUAGCAAGCUACGGUUACGAAAUUGUCUAGUUUCGUGACAAGAGAUUGAUAGACUCGCAAACUAAUGGCUUGAGUUAUGUGUCUUUAGUUUGUUAAAUGAAGACAUAACCUAUGGACGUAGACAAAUAUGUUGGCAGGUGUUUGGACGUGGGUUCGACUCCCACCAGCUCCACCA SEQ ID NO116Synechococcus sp.PCC6301 ssrAGGGGCTGTAATGGTTTCGACGTGTTGGTGAATCCTTCACCGTGATTCAGGCCGAGAGGGAGTCCACTCTCGTAAATCCAGGCTCAACCAAAAGTAACTGCGAACAACATCGTTCCTTTCGCTCGTAAGGCTGCTCCTGTAGCTGCTTAAACGCCACAAACTTTCTGGCTCGAGCGTCTAGTCGTAGACTCCGTTAATACGCCTAGACTTAAACCCCCAACGGATGCTCGAGTGGCGGCCTCAGGTCCGTCCTCTCGCTAAGCAAAAACCTGAGCATCCCGCCAACGGGGATAATCGTTGGCTCCCGCACAGTGGGTCAACCGTGCTAAGCCTGTGAACGAGCGGAAAGTTACTAGTCAATGCGGACAGCGGTTCGATTCCGCTCAGCTCCACCASEQ ID NO117Synechococcus sp.PCC6301 tmRNAGGGGCUGUAAUGGUUUCGACGUGUUGGUGAAUCCUUCACCGUGAUUCAGGCCGAGAGGGAGUCCACUCUCGUAAAUCCAGGCUCAACCAAAAGUAACUGCGAACAACAUCGUUCCUUUCGCUCGUAAGGCUGCUCCUGUAGCUGCUUAAACGCCACAAACUUUCUGGCUCGAGCGUCUAGUCGUAGACUCCGUUAAUACGCCUAGACUUAAACCCCCAACGGAUGCUCGAGUGGCGGCCUCAGGUCCGUCCUCUCGCUAAGCAAAAACCUGAGCAUCCCGCCAACGGGGAUAAUCGUUGGCUCCCGCACAGUGGGUCAACCGUGCUAAGCCUGUGAACGAGCGGAAAGUUACUAGUCAAUGCGGACAGCGGUUCGAUUCCGCUCAGCUCCACCA SEQ ID NO118Synechocystis sp.PCC6803 ssrAGGGGCCGCAATGGTTTCGACAGGTTGGCGAAAGCTTGCCCGTGATACAGGTCGAGAGTGAGTCTCCTCTCGCAAATCAAAGGCTCAAAAAAAAGTAACTGCGAATAACATCGTCAGCTTCAAACGGGTAGCCATAGCAGCCTAGTCTGTAAAAGCTACATTTTCTTGTCAAAGACCGTTTACTTCTTTTCTGACTCCGTTAAGGATTAGAGGTTAACCCCAACGGATGCTTTGTTTGGCTCTTCTCTAGTTAGCTAAACAATCAAGACTCAGACTAGAGCATCCCACCATCAGGGATAATCGATGGTCCCCGTCCTAGGGCTAGAAGGACTAAACCTGTGAATGAGCGGAAAGTTAATACCCAGTTTGGACAGCAGTTCAATTCTGCTCGGCTCCACCASEQ ID NO119Synechocystis sp.PCC6803 tmRNAGGGGCCGCAAUGGUUUCGACAGGUUGGCGAAAGCUUGCCCGUGAUACAGGUCGAGAGUGAGUCUCCUCUCGCAAAUCAAAGGCUCAAAAAAAAGUAACUGCGAAUAACAUCGUCAGCUUCAAACGGGUAGCCAUAGCAGCCUAGUCUGUAAAAGCUACAUUUUCUUGUCAAAGACCGUUUACUUCUUUUCUGACUCCGUUAAGGAUUAGAGGUUAACCCCAACGGAUGCUUUGUUUGGCUCUUCUCUAGUUAGCUAAACAAUCAAGACUCAGACUAGAGCAUCCCACCAUCAGGGAUAAUCGAUGGUCCCCGUCCUAGGGCUAGAAGGACUAAACCUGUGAAUGAGCGGAAAGUUAAUACCCAGUUUGGACAGCAGUUCAAUUCUGCUCGGCUCCACCA SEQ ID NO120海棲熱袍菌(Thermotoga maritima)ssrAGGGGGCGAACGGGTTCGACGGGGATGGAGTCCCCTGGGAAGCGAGCCGAGGTCCCCACCTCCTCGTAAAAAAGGTGGGACAAAGAATAAGTGCCAACGAACCTGTTGCTGTTGCCGCTTAATAGATAAGCGGCCGTCCTCTCCGAAGTTGGCTGGGCTTCGGAAGAGGGCGTGAGAGATCCAGCCTACCGATTCAGCTTCGCCTTCCGGCCTGAATCGGGAAAACTCAGGAAGGCTGTGGGAGAGGACACCCTGCCCGTGGGAGGTCCCTCCCGAGAGCGAAAACACGGGCTGCGCTCGGAGAAGCCCAGGGGCCTCCATCTTCGGACGGGGGTTCGAATCCCCCCGCCTCCACCA SEQ ID NO121海棲熱袍菌(Thermotoga maritima)tmRNAGGGGGCGAACGGGUUCGACGGGGAUGGAGUCCCCUGGGAAGCGAGCCGAGGUCCCCACCUCCUCGUAAAAAAGGUGGGACAAAGAAUAAGUGCCAACGAACCUGUUGCUGUUGCCGCUUAAUAGAUAAGCGGCCGUCCUCUCCGAAGUUGGCUGGGCUUCGGAAGAGGGCGUGAGAGAUCCAGCCUACCGAUUCAGCUUCGCCUUCCGGCCUGAAUCGGGAAAACUCAGGAAGGCUGUGGGAGAGGACACCCUGCCCGUGGGAGGUCCCUCCCGAGAGCGAAAACACGGGCUGCGCUCGGAGAAGCCCAGGGGCCUCCAUCUUCGGACGGGGGUUCGAAUCCCCCCGCCUCCACCA SEQ ID NO122嗜熱棲熱菌(Thermus thermophilus)ssrAGGGGGTGAAACGGTCTCGACGGGGGTCGCCGAGGGCGTGGCTGCGCGCCGAGGTGCGGGTGGCCTCGTAAAAACCCGCAACGGCATAACTGCCAACACCAACTACGCTCTCGCGGCTTAATGACCGCGACCTCGCCCGGTAGCCCTGCCGGGGGCTCACCGGAAGCGGGGACACAAACCCGGCTAGCCCGGGGCCACGCCCTCTAACCCCGGGCGAAGCTTGAAGGGGGCTCGCTCCTGGCCGCCCGTCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACTACGCGCGTAGAGGCCCGCCGTAGAGACCTTCGGACGGGGGTTCGACTCCCCCCACCTCCACCASEQ ID NO123嗜熱棲熱菌(Thermus thermophilus)tmRNAGGGGGUGAAACGGUCUCGACGGGGGUCGCCGAGGGCGUGGCUGCGCGCCGAGGUGCGGGUGGCCUCGUAAAAACCCGCAACGGCAUAACUGCCAACACCAACUACGCUCUCGCGGCUUAAUGACCGCGACCUCGCCCGGUAGCCCUGCCGGGGGCUCACCGGAAGCGGGGACACAAACCCGGCUAGCCCGGGGCCACGCCCUCUAACCCCGGGCGAAGCUUGAAGGGGGCUCGCUCCUGGCCGCCCGUCCGCGGGCCAAGCCAGGAGGACACGCGAAACGCGGACUACGCGCGUAGAGGCCCGCCGUAGAGACCUUCGGACGGGGGUUCGACUCCCCCCACCUCCACCA SEQ ID NO124蒼白密螺旋體(Treponema pallidum)ssrAGGGGATGACTAGGTTTCGACTAGGGATGTGGGGTGTTGCGCTGCAGGTGGAGTGTCGATCTCCTGATTCGGCGCCTTTATAACTGCCAATTCTGACAGTTTCGACTACGCGCTCGCCGCGTAATCGCGGGCCTGTGTTTGCGCTGCTCTGAGCGAACATATCGGCCCGACGCCAAACGGAGCTTGCTCTTACGTTGTGCACGGCGGACGTAGGGGGACTTTTGTCTGTGCTAAGACTCTGGCGCGTGCGGTGCAGGCCTAGCAGAGTCCGACAAACGCAGTACGCACCGCTAAACCTGTAGGCGCGCAGCACTCGCTCTTTAGGACGGGGGTTCGATTCCCCCCATCTCCACCASEQ ID NO125蒼白密螺旋體(Treponema pallidum)tmRNAGGGGAUGACUAGGUUUCGACUAGGGAUGUGGGGUGUUGCGCUGCAGGUGGAGUGUCGAUCUCCUGAUUCGGCGCCUUUAUAACUGCCAAUUCUGACAGUUUCGACUACGCGCUCGCCGCGUAAUCGCGGGCCUGUGUUUGCGCUGCUCUGAGCGAACAUAUCGGCCCGACGCCAAACGGAGCUUGCUCUUACGUUGUGCACGGCGGACGUAGGGGGACUUUUGUCUGUGCUAAGACUCUGGCGCGUGCGGUGCAGGCCUAGCAGAGUCCGACAAACGCAGUACGCACCGCUAAACCUGUAGGCGCGCAGCACUCGCUCUUUAGGACGGGGGUUCGAUUCCCCCCAUCUCCACCA SEQ ID NO126霍亂弧菌(Vibrio cholerae)ssrAGGGGCTGATTCAGGATTCGACGGGAATTTTGCAGTCTGAGGTGCATGCCGAGGTGCGGTAGGCCTCGTTAACAAACCGCAAAAAAATAGTCGCAAACGACGAAAACTACGCACTAGCAGCTTAATACCCTGCTCAGAGCCCTTCCTCCCTAGCTTCCGCTTGTAAGACGGGGAAATCAGGAAGGTCAAACCAAATCAAGCTGGCGTGGATTCCCCCACCTGAGGGATGAAGCGCGAGATCTAATTCAGGTTAGCCATTCGTTAGCGTGTCGGTTCGCAGGCGGTGGTGAAATTAAAGATCGACTAAGCATGTAGTACCAAAGATGAATGGTTTTCGGACGGGGGTTCAACTCCCCCCAGCTCCACCA SEQ ID NO127霍亂弧菌(Vibrio cholerae)tmRNAGGGGCUGAUUCAGGAUUCGACGGGAAUUUUGCAGUCUGAGGUGCAUGCCGAGGUGCGGUAGGCCUCGUUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAAAACUACGCACUAGCAGCUUAAUACCCUGCUCAGAGCCCUUCCUCCCUAGCUUCCGCUUGUAAGACGGGGAAAUCAGGAAGGUCAAACCAAAUCAAGCUGGCGUGGAUUCCCCCACCUGAGGGAUGAAGCGCGAGAUCUAAUUCAGGUUAGCCAUUCGUUAGCGUGUCGGUUCGCAGGCGGUGGUGAAAUUAAAGAUCGACUAAGCAUGUAGUACCAAAGAUGAAUGGUUUUCGGACGGGGGUUCAACUCCCCCCAGCUCCACCA SEQ ID NO128鼠疫耶爾森氏菌(Yersinia pestis)ssrAGGGGCTGATTCTGGATTCGACGGGATTCGCGAAACCCAAGGTGCATGCCGAGGTGCGGTGGCCTCGTAAAAAACCGCAAAAAAAATAGTTGCAAACGACGAAAACTACGCACTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTGCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCTAAAAGAGCTCGTGTGGAAACCTTGCCTGGGGTGGAAGCATTAAAACTAATCAGGATAGTTTGTCAGTAGCGTGTCCATCCGCAGCTGGCCGGCGAATGTAATGATTGGACTAAGCATGTAGTGCCGACGGTGTAGTAATTTCGGACGGGGGTTCAAATCCCCCCAGCTCCACCA SEQ ID NO129鼠疫耶爾森氏菌(Yersinia pestis)tmRNAGGGGCUGAUUCUGGAUUCGACGGGAUUCGCGAAACCCAAGGUGCAUGCCGAGGUGCGGUGGCCUCGUAAAAAACCGCAAAAAAAAUAGUUGCAAACGACGAAAACUACGCACUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUGCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCUAAAAGAGCUCGUGUGGAAACCUUGCCUGGGGUGGAAGCAUUAAAACUAAUCAGGAUAGUUUGUCAGUAGCGUGUCCAUCCGCAGCUGGCCGGCGAAUGUAAUGAUUGGACUAAGCAUGUAGUGCCGACGGUGUAGUAAUUUCGGACGGGGGUUCAAAUCCCCCCAGCUCCACCA SEQ ID NO130胎兒彎曲桿菌(Campylobacter fetus)ssrA，內(nèi)部部分AGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATCTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAAGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGSEQ ID NO131胎兒彎曲桿菌(Campylobacter fetus)tmRNA，內(nèi)部部分AGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUCUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAAGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGG SEQ ID NO132大腸彎曲桿菌(Campylobacter coli)(BM2509)ssrA，內(nèi)部部分AGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGACAAAGCGTAAAAAGTCCAAATTAAAATTAAACGCAAATAACGTTAAATTTGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCCGAAACTCAAGTGATGCTATCTAGCTTGAATTTTGGTCATCTTTGATAGTGTAGATTGAAAATTGACAACTTTTAATCGAAGTTAAAGTCTTAGTCTAGCTTGAAATTTTGGAAGGTGAGTTTAGCCAGATGAAGTTTTCACCTTTGCTAAACATGTAGAAGTCTTTGTGGGGTTATTTTTGGSEQ ID NO133大腸彎曲桿菌(Campylobacter coli)(BM2509)tmRNA，內(nèi)部部分AGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGACAAAGCGUAAAAAGUCCAAAUUAAAAUUAAACGCAAAUAACGUUAAAUUUGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCCGAAACUCAAGUGAUGCUAUCUAGCUUGAAUUUUGGUCAUCUUUGAUAGUGUAGAUUGAAAAUUGACAACUUUUAAUCGAAGUUAAAGUCUUAGUCUAGCUUGAAAUUUUGGAAGGUGAGUUUAGCCAGAUGAAGUUUUCACCUUUGCUAAACAUGUAGAAGUCUUUGUGGGGUUAUUUUUGG SEQ ID NO134雞彎曲桿菌分離物(Camplyobacter chicken isolate)ssrA，內(nèi)部部分ACAGGAGTAAGTCTGCTTAGATGGCATGTCGCTTTGGGCAAAGCGTAAAAAGCCCAAATAAAATTAAACGCAAACAACGTTAAATTCGCTCCTGCTTACGCTAAAGCTGCGTAAGTTCAGTTGAGCCTGAAATTTAAGTCATACTATCTAGCTTAATTTTCGGTCATTTTTGATAGTGTAGCCTTGCGTTTGACAAGCGTTGAGGTGAAATAAGGTCTTAGCCTTGCTTTTGAGTTTTGGAAGATGAGCGAAGTAGGGTGAAGTAGTCATCTTTGCTAAGCATGTAGAGGTCTTTGTGGGATTATTTTTGGSEQ ID NO135雞彎曲桿菌分離物(Camplyobacter chicken isolate)tmRNA，內(nèi)部部分ACAGGAGUAAGUCUGCUUAGAUGGCAUGUCGCUUUGGGCAAAGCGUAAAAAGCCCAAAUAAAAUUAAACGCAAACAACGUUAAAUUCGCUCCUGCUUACGCUAAAGCUGCGUAAGUUCAGUUGAGCCUGAAAUUUAAGUCAUACUAUCUAGCUUAAUUUUCGGUCAUUUUUGAUAGUGUAGCCUUGCGUUUGACAAGCGUUGAGGUGAAAUAAGGUCUUAGCCUUGCUUUUGAGUUUUGGAAGAUGAGCGAAGUAGGGUGAAGUAGUCAUCUUUGCUAAGCAUGUAGAGGUCUUUGUGGGAUUAUUUUUGG SEQ ID NO136產(chǎn)氣莢膜梭菌(Clostridium perfringens)ssrA，內(nèi)部部分ACGGGGGTAGGATGGGTTTGATAAGCGAGTCGAGGGAAGCATGGTGCCTCGATAATAAAGTATGCATTAAAGATAAACGCACGAGATAATTTTGCATTAGCAGCTTAAGTTAGCGCTGCTCATCCTTCCTCAATTGCCCACGGTTGAGAGTAAGGGTGTCATTTAAAAGTGGGGAACCGAGCCTAGCAAAGCTTTGAGCTAGGAACGGAATTTATGAAGCTTACCAAAGAGGAAGTTTGTCTGTGGACGTTCTCTGAGGGAATTTTAAAACACAAGACTACACTCGTAGAAAGTCTTACTGGTCTGCTTTCGG SEQ ID NO137產(chǎn)氣莢膜梭菌(Clostridium perfringens)tmRNA，內(nèi)部部分ACGGGGGUAGGAUGGGUUUGAUAAGCGAGUCGAGGGAAGCAUGGUGCCUCGAUAAUAAAGUAUGCAUUAAAGAUAAACGCACGAGAUAAUUUUGCAUUAGCAGCUUAAGUUAGCGCUGCUCAUCCUUCCUCAAUUGCCCACGGUUGAGAGUAAGGGUGUCAUUUAAAAGUGGGGAACCGAGCCUAGCAAAGCUUUGAGCUAGGAACGGAAUUUAUGAAGCUUACCAAAGAGGAAGUUUGUCUGUGGACGUUCUCUGAGGGAAUUUUAAAACACAAGACUACACUCGUAGAAAGUCUUACUGGUCUGCUUUCGG SEQ ID NO138杜氏嗜血菌(Haemophilus ducreyi)(NCTC 10945)ssrA，內(nèi)部部分ACGGGATTAGCGAAGTCCAAGGTGCACGTCGAGGTGCGGTAGGCCTCGTAACAAACCGCAAAAAAATAGTCGCAAACGACGAACAATACGCTTTAGCAGCTTAATAACCTGCATTTAGCCTTCGCGCCCTAGCTTTCGCTCGTAAGACGGGGAGCACGCGGAGTCAAACCAAAACGAGATCGTGTGGACGCTTCCGCTTGTAGATGAAACACTAAATTGAATCAAGCTAGTTTATTTCTTGCGTGTCTGTCCGCTGGAGATAAGCGAAATTAAAGACCAGACTAAACGTGTAGTACTGAAGATAGAGTAATTTCGGACCCGGGTTCGACTC SEQ ID NO139杜氏嗜血菌(Haemophilus ducreyi)(NCTC 10945)tmRNA，內(nèi)部部分ACGGGAUUAGCGAAGUCCAAGGUGCACGUCGAGGUGCGGUAGGCCUCGUAACAAACCGCAAAAAAAUAGUCGCAAACGACGAACAAUACGCUUUAGCAGCUUAAUAACCUGCAUUUAGCCUUCGCGCCCUAGCUUUCGCUCGUAAGACGGGGAGCACGCGGAGUCAAACCAAAACGAGAUCGUGUGGACGCUUCCGCUUGUAGAUGAAACACUAAAUUGAAUCAAGCUAGUUUAUUUCUUGCGUGUCUGUCCGCUGGAGAUAAGCGAAAUUAAAGACCAGACUAAACGUGUAGUACUGAAGAUAGAGUAAUUUCGGACCCGGGUUCGACUCSEQ ID NO140無害李斯特氏菌(Listeria innocua)(食物分離物#1)ssrA，內(nèi)部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAACCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTCAA SEQ ID NO141無害李斯特氏菌(Listeria innocua)(食物分離物 #1)tmRNA，內(nèi)部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAACCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUCAA SEQ ID NO142無害李斯特氏菌(Listeria innocua)(食物分離物 #2)ssrA，內(nèi)部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA SEQ ID NO143無害李斯特氏菌(Listeria innocua)(食物分離物#2)tmRNA，內(nèi)部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA SEQ ID NO144無害李斯特氏菌(Listeria innocua)(食物分離物#3)ssrA，內(nèi)部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGAATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGAGGTTAAATAGAAGAGCTTAATCGGACTAGCTGAATGGAAGCCTGTTACCGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA SEQ ID NO145無害李斯特氏菌(Listeria innocua)(食物分離物 #3)tmRNA，內(nèi)部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGAAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGAGGUUAAAUAGAAGAGCUUAAUCGGACUAGCUGAAUGGAAGCCUGUUACCGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA SEQ ID NO146無害李斯特氏菌(Listeria innocua)(ATCC 12210)ssrA，內(nèi)部部分GGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTCTAAGTGGGCTACACTAGTTAATCTCCGTCTGGGGTTAAATAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACTGGGCCGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAA SEQ ID NO147無害李斯特氏菌(Listeria innocua)(ATCC 12210)tmRNA，內(nèi)部部分GGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUCUAAGUGGGCUACACUAGUUAAUCUCCGUCUGGGGUUAAAUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACUGGGCCGAUGUUUAUGCGAAAUGCUAAUACGGUGACUACGCUCGUAGAUAUUUAA SEQ ID NO148伊氏李斯特氏菌(Listeria ivarnovii)(NCTC 11846)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATTAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO149伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUUAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUCGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO150斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGAAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATACTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCCATATTTCTGG SEQ ID NO151斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGAAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUACUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCCAUAUUUCUGG SEQ ID NO152腸炎沙門氏菌(Salmonella enteritidis)ssrA，內(nèi)部部分ACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGG SEQ ID NO153腸炎沙門氏菌(Salmonella enteritidis)tmRNA，內(nèi)部部分ACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGG SEQ ID NO154表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)ssrA，內(nèi)部部分ACAGGGGTCCCCCGAGCTTATTAAGCGTGTCGGAGGGTTGGCTCCGTCATCAACACATTTCGGTTAAATATAACTGACAAATCAAACAATAATTTCGCAGTAGCTGCGTAATAGCCACTGCATCGCCTAACAGCATCTCCTACGTGCTGTTAACGCGATTCAACCCTAGTAGGATATGCTAAACACTGCCGCTTGAAGTCTGTTTAGATGAAATATAATCAAGCTAGTATCATGTTGGTTGTTTATTGCTTAGCATGATGCGAAAATTATCAATAAACTACACACGTAGAAAGATTTGTATCAGGACCTCTGGSEQ ID NO155表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)tmRNA，內(nèi)部部分ACAGGGGUCCCCCGAGCUUAUUAAGCGUGUCGGAGGGUUGGCUCCGUCAUCAACACAUUUCGGUUAAAUAUAACUGACAAAUCAAACAAUAAUUUCGCAGUAGCUGCGUAAUAGCCACUGCAUCGCCUAACAGCAUCUCCUACGUGCUGUUAACGCGAUUCAACCCUAGUAGGAUAUGCUAAACACUGCCGCUUGAAGUCUGUUUAGAUGAAAUAUAAUCAAGCUAGUAUCAUGUUGGUUGUUUAUUGCUUAGCAUGAUGCGAAAAUUAUCAAUAAACUACACACGUAGAAAGAUUUGUAUCAGGACCUCUGG SEQ ID NO156無乳鏈球菌(Streptococcus agalactiae)(NCTC 8181)ssrA，內(nèi)部部分ACAGGCATTATGAGGTATATTTTGCGACTCATCGGCAGATGTAAAATGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCATTAGCTGCCTAAAAAACAGCCTGCGTGATCTTCACAAGATTGTTTGCGTTTTGCTAGAAGGTCTTATTTATCAGCAAACTACGTTTGGCTACTGTCTAGTTAGTTAAAAAGAGATTTATAGACTCGCTATGTGAGGGCTTGAGTTATGTGTCATCACCTAGTTAAATCAATACATAACCTATAGTTGTAGACAAATATATTAGCAGATGTTTGGSEQ ID NO157無乳鏈球菌(Streptococcus agalactiae)(NCTC 8181)tmRNA，內(nèi)部部分ACAGGCAUUAUGAGGUAUAUUUUGCGACUCAUCGGCAGAUGUAAAAUGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAUUAGCUGCCUAAAAAACAGCCUGCGUGAUCUUCACAAGAUUGUUUGCGUUUUGCUAGAAGGUCUUAUUUAUCAGCAAACUACGUUUGGCUACUGUCUAGUUAGUUAAAAAGAGAUUUAUAGACUCGCUAUGUGAGGGCUUGAGUUAUGUGUCAUCACCUAGUUAAAUCAAUACAUAACCUAUAGUUGUAGACAAAUAUAUUAGCAGAUGUUUGG SEQ ID NO158支氣管炎博德特氏菌(Bordetella bronchiseptica)ssrAGGGGCCGATCCGGATTCGACGTGGGTCATGAAACAGCTCAAGGCATGCCGAGCACCAGTAAGCTCGTTAATCCACTGGAACACTACAAACGCCAACGACGAGCGTTTCGCTCTCGCCGCTTAAGCGGTGAGCCGCTGCACTGATCTGTCCTTGGGTCACGCGGGGGAASEQ ID NO159支氣管炎博德特氏菌(Bordetella bronchiseptica)tmRNAGGGGCCGAUCCGGAUUCGACGUGGGUCAUGAAACAGCUCAAGGCAUGCCGAGCACCAGUAAGCUCGUUAAUCCACUGGAACACUACAAACGCCAACGACGAGCGUUUCGCUCUCGCCGCUUAAGCGGUGAGCCGCUGCACUGAUCUGUCCUUGGGUCACGCGGGGGAASEQ ID NO160肺炎衣原體(Chlamydia pneumoniae)(CWL029)，ssrAGGGGGTGTATAGGTTTCGACTTGAAAATGAAGTGTTAATTGCATGCGGAGGGCGTTGGCTGGCCTCCTAAAAAGCCAACAAAACAATAAATGCCGAACCTAAGGCTGAATGCGAAATTATTAGCTTGTTTGACTCAGTAGAGGAAAGACTAGCTGCTTAATTAGCAAAAGTTGTTAGCTAGATAATCTCTAGGTAACCCGGTATCTGCGAGCTCCACCAGAGGCTTGCAAAATACCGTCATTTATCTGGTTGGAACTTACTTTCTCTAATTCTCAAGGAAGTTCGTTCGAGATTTTTGAGAGTCATTGGCTGCTATAGAGGCTTCTAGCTAAGGGAGTCCAATGTAAACAATTCTAGAAGATAAGCATGTAGAGGTTAGCAGGGAGTTTGTCAAGGACGAGAGTTCGAGTCTCTCCACCTCCACCA SEQ ID NO161肺炎衣原體(Chlamydia pneumoniae)(CWL029)tmRNAGGGGGUGUAUAGGUUUCGACUUGAAAAUGAAGUGUUAAUUGCAUGCGGAGGGCGUUGGCUGGCCUCCUAAAAAGCCAACAAAACAAUAAAUGCCGAACCUAAGGCUGAAUGCGAAAUUAUUAGCUUGUUUGACUCAGUAGAGGAAAGACUAGCUGCUUAAUUAGCAAAAGUUGUUAGCUAGAUAAUCUCUAGGUAACCCGGUAUCUGCGAGCUCCACCAGAGGCUUGCAAAAUACCGUCAUUUAUCUGGUUGGAACUUACUUUCUCUAAUUCUCAAGGAAGUUCGUUCGAGAUUUUUGAGAGUCAUUGGCUGCUAUAGAGGCUUCUAGCUAAGGGAGUCCAAUGUAAACAAUUCUAGAAGAUAAGCAUGUAGAGGUUAGCAGGGAGUUUGUCAAGGACGAGAGUUCGAGUCUCUCCACCUCCACCASEQ ID NO162土拉熱弗朗西絲氏菌(Francisella tularensis)ssrAGGGGGCGAATATGGTTTCGACATGAATGTCAAAATCTAAGGTGCATGCCGAGGAAGTACCGTAACCTCGTTAATAACAGTACAAATGCCAATAATAACTGGCAACAAAAAAGCAAACCGCGTAGCGGCTAACGACAGCAACTTTGCTGCTGTTGCTAAAGCTGCCTAGTCTAGCTTAATAATCTAGATGCGCACGGATATGATAGTCTTTCTTATGACACTATCTATACATCCGTTCATATTCCGCATAAGACGGTCTTTGCTTTTTGTCTGGGAGTTAAGGCTGTATTTAACAGACTCGCTAACTATTACCCTGGCTAATTGGGGAATAGTCAAGCTAAACTCAAATAGATTAGCCTAAGCATGTAGATCCAAAGATCTAGAGTTTGTGGACGCGGGTTCAAATCCCGCCGCCTCCACCA SEQ ID NO163土拉熱弗朗西絲氏菌(Francisella tularensis)tmRNAGGGGGCGAAUAUGGUUUCGACAUGAAUGUCAAAAUCUAAGGUGCAUGCCGAGGAAGUACCGUAACCUCGUUAAUAACAGUACAAAUGCCAAUAAUAACUGGCAACAAAAAAGCAAACCGCGUAGCGGCUAACGACAGCAACUUUGCUGCUGUUGCUAAAGCUGCCUAGUCUAGCUUAAUAAUCUAGAUGCGCACGGAUAUGAUAGUCUUUCUUAUGACACUAUCUAUACAUCCGUUCAUAUUCCGCAUAAGACGGUCUUUGCUUUUUGUCUGGGAGUUAAGGCUGUAUUUAACAGACUCGCUAACUAUUACCCUGGCUAAUUGGGGAAUAGUCAAGCUAAACUCAAAUAGAUUAGCCUAAGCAUGUAGAUCCAAAGAUCUAGAGUUUGUGGACGCGGGUUCAAAUCCCGCCGCCUCCACCASEQ ID NO164Guillardia theta(質(zhì)體)ssrAGGGGCTGATTTGGATTCGACATATAAATTTGCGTGTTTCATTATGAAGCAAGTCAAGTTTAATGATCTTGTAAAAAACATTAAAGTACAAATAAATGCAAGCAATATAGTTTCATTTAGTTCAAAACGTTTAGTCTCTTTTGCATAAGCAAAATGTGTTAATAACTTTCTTAGTAGAAATTGGAGAAGTTTACTAAGATTTATATTTACTCCATAATTATTTTAAAGATGGTAAAAAGGTGATTCATCATTTGTATGTTTCTAAACTTTGTGAAAGAATAGTGGGCTCCATTTATAATGAACGTGGGTTCAAATCCCACCAGCTCCACCA SEQ ID NO165Guillardia theta(質(zhì)體)tmRNAGGGGCUGAUUUGGAUUCGACAUAUAAAUUUGCGUGUUUCAUUAUGAAGCAAGUCAAGUUUAAUGAUCUUGUAAAAAACAUUAAAGUACAAAUAAAUGCAAGCAAUAUAGUUUCAUUUAGUUCAAAACGUUUAGUCUCUUUUGCAUAAGCAAAAUGUGUUAAUAACUUUCUUAGUAGAAAUUGGAGAAGUUUACUAAGAUUUAUAUUUACUCCAUAAUUAUUUUAAAGAUGGUAAAAAGGUGAUUCAUCAUUUGUAUGUUUCUAAACUUUGUGAAAGAAUAGUGGGCUCCAUUUAUAAUGAACGUGGGUUCAAAUCCCACCAGCUCCACCASEQ ID NO166Thalassiosira Weissflogii(質(zhì)體)ssrAGGGGCTGATTTGGTTTCGACATTTAAAACTTCTTTCTATGTGTCAGGTCAAAGTTTGTATTCTTTGTAAAAAAATACTAAAATACTAATAAATGCTAATAATATAATACCGTTTATTTTTAAAGCAGTAAAAACAAAAAAAGAAGCAATGGCTTTAAATTTTGCTGTATAGTTCATTAACTTAGGTTATTAAATATTTTTTCATTATAACTGGACTTTTCTCTAGTTTATAGTTTAGAATAAATTTAAATTTTGCAAAACTCGTTCGAAAATTTTCGGGCTAAACCTGTAAACGCAAATACTAAGAAATTTTAGATGGACATGGGTTCAATTCCCATCAGTTCCACCASEQ ID NO167Thalassiosira Weissflogii(質(zhì)體)tmRNAGGGGCUGAUUUGGUUUCGACAUUUAAAACUUCUUUCUAUGUGUCAGGUCAAAGUUUGUAUUCUUUGUAAAAAAAUACUAAAAUACUAAUAAAUGCUAAUAAUAUAAUACCGUUUAUUUUUAAAGCAGUAAAAACAAAAAAAGAAGCAAUGGCUUUAAAUUUUGCUGUAUAGUUCAUUAACUUAGGUUAUUAAAUAUUUUUUCAUUAUAACUGGACUUUUCUCUAGUUUAUAGUUUAGAAUAAAUUUAAAUUUUGCAAAACUCGUUCGAAAAUUUUCGGGCUAAACCUGUAAACGCAAAUACUAAGAAAUUUUAGAUGGACAUGGGUUCAAUUCCCAUCAGUUCCACCA SEQ ID NO168幽門螺桿菌(Helicobacter pylori)ssrA，(臨床分離物1)，內(nèi)部部分TGGGGATGTTACGGTTTCGACAGGGGTAGTTCGAGCTTAGGTGGCGAGTCGAGGGGATCGGCCTCGTTAAAACGTCAAAGCCTATAACTGGCAAACAACAAAACAACTTCGCTTTAGCAGCTTAATAAGCTCTTAGCGGTTCCTCCCTCCATCGCCCATGTGGTAGGGTAAGGGACTCAAATTAAGTGGGCTACGCTGGATTCCACCGTCTGAGGATGAAAGAAGAGAACAACCAGACTAGCTACCCGGACGCCCGTCGATAGGCAGATGGAGTAGCGAATCGCGAATATATCGACTACACTCGTAGAAGCTTAAGTGCCGATATTCTTGGACGTGGGTTCGACTCCCSEQ ID NO176幽門螺桿菌(Helicobacter pylori)tmRNA，(臨床分離物1)，內(nèi)部部分UGGGGAUGUUACGGUUUCGACAGGGGUAGUUCGAGCUUAGGUGGCGAGUCGAGGGGAUCGGCCUCGUUAAAACGUCAAAGCCUAUAACUGGCAAACAACAAAACAACUUCGCUUUAGCAGCUUAAUAAGCUCUUAGCGGUUCCUCCCUCCAUCGCCCAUGUGGUAGGGUAAGGGACUCAAAUUAAGUGGGCUACGCUGGAUUCCACCGUCUGAGGAUGAAAGAAGAGAACAACCAGACUAGCUACCCGGACGCCCGUCGAUAGGCAGAUGGAGUAGCGAAUCGCGAAUAUAUCGACUACACUCGUAGAAGCUUAAGUGCCGAUAUUCUUGGACGUGGGUUCGACUCCC SEQ ID NO177幽門螺桿菌(Helicobacter pylori)ssrA，(臨床分離物2)，內(nèi)部部分TGGGGACGTTACGGTTTCGACAGGGATAGTTCGAGCTTAGGTTGCGAGTCGAGGGGATCGGCCTCGTTAAAACGTCAAAGCCTATAATTGGCAAACAAAACAATCTTTCTTTAGCTGCTTAATTGCACTAAAGGTTCCTCCCTCCATCGTCCATGTGGTAGGGTAAGGGACTCAAACTAAGTGGACTACGCCGGAGTTCGCCGTCTGAGGACAAAGGAAGAGAACAACCAGACTAGCAACTTGGAAGCCTGTCGATAGGCCGAAGAGTTCGCGAAATGCTAATATATCGACTACACTCGTAGAAGCTTAAGTGCCGATATTTTTGGACGTGGGTTCGATTCCCT SEQ ID NO178幽門螺桿菌(Helicobacter pylori)tmRNA，(臨床分離物2)，內(nèi)部部分UGGGGACGUUACGGUUUCGACAGGGAUAGUUCGAGCUUAGGUUGCGAGUCGAGGGGAUCGGCCUCGUUAAAACGUCAAAGCCUAUAAUUGGCAAACAAAACAAUCUUUCUUUAGCUGCUUAAUUGCACUAAAGGUUCCUCCCUCCAUCGUCCAUGUGGUAGGGUAAGGGACUCAAACUAAGUGGACUACGCCGGAGUUCGCCGUCUGAGGACAAAGGAAGAGAACAACCAGACUAGCAACUUGGAAGCCUGUCGAUAGGCCGAAGAGUUCGCGAAAUGCUAAUAUAUCGACUACACUCGUAGAAGCUUAAGUGCCGAUAUUUUUGGACGUGGGUUCGAUUCCCUSEQ ID NO179斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATCAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGAAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATACTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCCATATTTCTGG SEQ ID NO180斯氏李斯特氏菌(Listeria seeligeri)(NCTC 11856)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUCAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGAAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUACUAAUACGGUGACUACGCUCGUAGAUAUUUAAGUGCCCAUAUUUCUGG SEQ ID NO181伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)ssrA，內(nèi)部部分ACAGGGATAGTTCGAGCTTGAGTTGCGAGTCGGGGGGATCGTCCTCGTTATTAACGTCAAAGCCAATAATAACTGGCAAAGAAAAACAAAACCTAGCTTTCGCTGCCTAATAAGCAGTAGCATAGCTGATCCTCCGTGCATCGCCCATGTGCTACGGTAAGGGTCTCACTTTAAGTGGGCTACACTAAATAATCTCCGTCTGGGGTTAGTTAGAAGAGCTTAATCAGACTAGCTGAATGGAAGCCTGTTACCGGGCTGATGTTTATGCGAAATGCTAATACGGTGACTACGCTCGTAGATATTTAAGTGCCGATATTTCTGG SEQ ID NO182伊氏李斯特氏菌(Listeria ivanovii)(NCTC 11846)tmRNA，內(nèi)部部分ACAGGGAUAGUUCGAGCUUGAGUUGCGAGUCGGGGGGAUCGUCCUCGUUAUUAACGUCAAAGCCAAUAAUAACUGGCAAAGAAAAACAAAACCUAGCUUUCGCUGCCUAAUAAGCAGUAGCAUAGCUGAUCCUCCGUGCAUCGCCCAUGUGCUACGGUAAGGGUCUCACUUUAAGUGGGCUACACUAAAUAAUCUCCGUCUGGGGUUAGUUAGAAGAGCUUAAUCAGACUAGCUGAAUGGAAGCCUGUUACCGGGCUGAUGUUUAUGCGAAAUGCUAAUACGGUGACUCGCUCGUAGAUAUUUAAGUGCCGAUAUUUCUGG SEQ ID NO183非洲分枝桿菌(Mycobacterium africanum)(臨床分離物)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAACCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO184非洲分枝桿菌(Mycobacterium africanum)(臨床分離物)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAACCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO185戈登氏分枝桿菌(Mycobacterium gordonae)(臨床分離物)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCATATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCGGACCGGGAACGCCCTCGCCCCGGACCCCGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTCCGTGTGACCAGGAGATCCGAGCAGAGACATAGCGGACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO186戈登氏分枝桿菌(Mycobacterium gordonae)(臨床分離物)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAUAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCGGACCGGGAACGCCCUCGCCCCGGACCCCGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUCCGUGUGACCAGGAGAUCCGAGCAGAGACAUAGCGGACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO187堪薩斯分枝桿菌(Mycobacterium kansasii)(臨床分離物)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGACCGCCCTCGACCCGGACTCTGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCGTCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO188堪薩斯分枝桿菌(Mycobacterium kansasii)(臨床分離物)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGACCGCCCUCGACCCGGACUCUGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCGUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO189龜分枝桿菌(Mycobacterium chelonae)ssrA，內(nèi)部部分ACAGCGAGTCTCGACTTAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCATTGCAACCAATTAAGCGCCGATTCTCATCAGCGCGACTACGCACTCGCTGCCTAAGCGACTGCGTGTCTGTCAGACCGGGAGCGCCCTCAGCCCGGACCCTGGCATCAGCTAGAGGGACAAACTACGGGTTCGGTCGCGGGACCCGTAGGGACATCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGGGACCGAGAGATCCAAGTAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTAATGAACGGCCGTTG SEQ ID NO190龜分枝桿菌(Mycobacterium chelonae)tmRNA，內(nèi)部部分ACAGCGAGUCUCGACUUAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCAUUGCAACCAAUUAAGCGCCGAUUCUCAUCAGCGCGACUACGCACUCGCUGCCUAAGCGACUGCGUGUCUGUCAGACCGGGAGCGCCCUCAGCCCGGACCCUGGCAUCAGCUAGAGGGACAAACUACGGGUUCGGUCGCGGGACCCGUAGGGACAUCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGGGACCGAGAGAUCCAAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUAAUGAACGGCCGUUG SEQ ID NO191斯氏分枝桿菌(蘇加分枝桿菌)(Mycobacterium szulgai)(ATCC 35799)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGAGAACACTCAGCGCGACTTCGCTCTCGCTGCCTAAGCGACAGCAAGTCCGTCAGACCGGGAAAGCCCTCGACCCGGACCCTGGCGTCATCTAGAGGGATCCACCGGTGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG SEQ ID NO192斯氏分枝桿菌(蘇加分枝桿菌)(Mycobacterium szulgai)(ATCC 35799)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAGAACACUCAGCGCGACUUCGCUCUCGCUGCCUAAGCGACAGCAAGUCCGUCAGACCGGGAAAGCCCUCGACCCGGACCCUGGCGUCAUCUAGAGGGAUCCACCGGUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG SEQ ID NO193瑪爾摩分枝桿菌(Mycobacterium malmoense)(臨床分離物)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCATATAAGCGCCGTTTCAACACAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTGGAGGGATCCACCGGTGAGTCCGGTCGCGGGACTCATCGGGACATACACAGCGACTGGGATCGTCATCCTGGCTGGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG SEQ ID NO194瑪爾摩分枝桿菌(Mycobacterium malmoense)(臨床分離物)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAUAUAAGCGCCGUUUCAACACAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUGGAGGGAUCCACCGGUGAGUCCGGUCGCGGGACUCAUCGGGACAUACACAGCGACUGGGAUCGUCAUCCUGGCUGGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG SEQ ID NO195微黃分枝桿菌(Mycobacterium flavescens)ssrA，內(nèi)部部分ACTTCGAGCGTCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCACTCGCTGCCTAAGCGACTGCGTGTCTGTCAGCCCGGGAGAGCCCTCGACCCGGTGTCTGGCATCAGCTAGAGGGATAAACCGGTGGGTCCGGTCGCGGGACTCATCGGGACATCAAACAGCGACTGGGATCGTCATCCTGACTTGTTCGCGTGATCAGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAACGCCGTAG SEQ ID NO196微黃分枝桿菌(Mycobacterium flavescens)tmRNA，內(nèi)部部分ACUUCGAGCGUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCACUCGCUGCCUAAGCGACUGCGUGUCUGUCAGCCCGGGAGAGCCCUCGACCCGGUGUCUGGCAUCAGCUAGAGGGAUAAACCGGUGGGUCCGGUCGCGGGACUCAUCGGGACAUCAAACAGCGACUGGGAUCGUCAUCCUGACUUGUUCGCGUGAUCAGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAACGCCGUAG SEQ ID NO197海分枝桿菌(Mycobacterium marinum)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGATGCAACTAGATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCGGACCGGGAACGCCCTCGCCCCGGACCCCGGCATCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGGCTCATCGGGACATCAACAGCGACTGGGATCGTCATCCTGGCTAGTTCGCGTGACCAGGAGATCCGAGCAGAGACCTAGCGGACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTAG SEQ ID NO198海分枝桿菌(Mycobacterium marinum)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGAUGCAACUAGAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCGGACCGGGAACGCCCUCGCCCCGGACCCCGGCAUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGGCUCAUCGGGACAUCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUUCGCGUGACCAGGAGAUCCGAGCAGAGACCUAGCGGACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUAG SEQ ID NO199田鼠分枝桿菌(Mycobacterium microti)(環(huán)境分離物)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCGACCAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTCCTCGGGACAGCCACAGCGACTGGGATCGTCATCTCGGCTAGTTCGCGTGACCGGGAGATCCGAGCAGAGGCATAGCGAACTGCGCACGGAGAAGCCTTGAGGGAATGCCGTA SEQ ID NO200田鼠分枝桿菌(Mycobacterium microti)(環(huán)境分離物)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCGACCAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACGGCUAGUCUGUCAGACCGGGAACGCCCUCGGCCCGGACCCUGGCAUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUCCUCGGGACAGCCACAGCGACUGGGAUCGUCAUCUCGGCUAGUUCGCGUGACCGGGAGAUCCGAGCAGAGGCAUAGCGAACUGCGCACGGAGAAGCCUUGAGGGAAUGCCGUA SEQ ID NO201恥垢分枝桿菌(Mycobacterium smegmatis)(ATCC 10143)ssrA，內(nèi)部部分ACTTCGAGCATCGAATCCAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCCCTCGCTGCCTAAGCGACGGCTGGTCTGTCAGACCGGGAGTGCCCTCGGCCCGGATCCTGGCATCAGCTAGAGGGACCCACCCACGGGTTCGGTCGCGGGACCTGTGGGGACATCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGTAGAGACATAGCGAACTGCGCACGGAGAAGCCTCGAGGACATGCCGTAG SEQ ID NO202恥垢分枝桿菌(Mycobacterium smegmatis)(ATCC 10143)ssrA，內(nèi)部部分ACUUCGAGCAUCGAAUCCAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCCCUCGCUGCCUAAGCGACGGCUGGUCUGUCAGACCGGGAGUGCCCUCGGCCCGGAUCCUGGCAUCAGCUAGAGGGACCCACCCACGGGUUCGGUCGCGGGACCUGUGGGGACAUCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGUGUGACCGGGAGAUCCGAGUAGAGACAUAGCGAACUGCGCACGGAGAAGCCUCGAGGACAUGCCGUAG SEQ ID NO203蟾(蜍)分枝桿菌(Mycobacterium xenopi)(臨床分離物)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACTAAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGGCCGGGAGTTCCCTCGACCCGGATCCTGGCGTCAGCTAGAGGGATCCACCGATGGGTTCGGTCGCGGGACCCATCGGGACACCACACAGCGACTGGGATCGCCGTCCCGGCTAGTTCGCGAGACCGGGAGATCCGAGTAAGGGCAAAGCGAACTGCGCACGGAGAAGCCTTGAGGGTATGCCGTA SEQ ID NO204蟾(蜍)分枝桿菌(Mycobacterium xenopi)(臨床分離物)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACUAAAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGGCCGGGAGUUCCCUCGACCCGGAUCCUGGCGUCAGCUAGAGGGAUCCACCGAUGGGUUCGGUCGCGGGACCCAUCGGGACACCACACAGCGACUGGGAUCGCCGUCCCGGCUAGUUCGCGAGACCGGGAGAUCCGAGUAAGGGCAAAGCGAACUGCGCACGGAGAAGCCUUGAGGGUAUGCCGUA SEQ ID NO205胞內(nèi)分枝桿菌(Mycobacterium intracellulare)(NCTC 10425)ssrA，內(nèi)部部分ACTTCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAACCGACCACCGTAAGCGTCGTTGCAAACAGATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAACGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCCACCGATGAGTCCGGTCGCGGGACTTATCGGGACACCAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGTGACCGGGAGATCCGAGTAGAGGCATAGCGAACTGCGCACGGAGAAGTCTTGAGGGAATGCCGTAG SEQ ID NO206胞內(nèi)分枝桿菌(Mycobacterium intracellulare)(NCTC 10425)tmRNA，內(nèi)部部分ACUUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAACCGACCACCGUAAGCGUCGUUGCAAACAGAUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAACGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCCACCGAUGAGUCCGGUCGCGGGACUUAUCGGGACACCAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGUGACCGGGAGAUCCGAGUAGAGGCAUAGCGAACUGCGCACGGAGAAGUCUUGAGGGAAUGCCGUAG SEQ ID NO207淋巴腺炎分枝桿菌(瘰疬分枝桿菌)(Mycobacterium scrofulaceum)(NCTC 10803)ssrA，內(nèi)部部分ACATCGCGCATCGAATCAAGGGAAGCGTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCAACCAATTAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCCTAAGCGACAGCTAGTCCGTCAGACCGGGAAAGCCCTCGACCCGGAGCCTGGCGTCAGCTAGAGGGATCAACCGATGAGTTCGGTCGCGGGACTCATCGGGACACCAACAGCGACTGGGATCGTCATCCTGGCTAGTCCGCGTGACCAGGAGATCCGAGCAGAGGCATAGCGGACTGCGCACGGAGAAGTCTTGAGGGAATGCCGTTG SEQ ID NO208淋巴腺炎分枝桿菌(瘰疬分枝桿菌)(Mycobacterium scrofulaceum)(NCTC 10803)tmRNA，內(nèi)部部分ACAUCGCGCAUCGAAUCAAGGGAAGCGUGCCGGUGCAGGCAAGAGACCACCGUAAGCGUCGUUGCAACCAAUUAAGCGCCGAUUCACAUCAGCGCGACUACGCUCUCGCUGCCUAAGCGACAGCUAGUCCGUCAGACCGGGAAAGCCCUCGACCCGGAGCCUGGCGUCAGCUAGAGGGAUCAACCGAUGAGUUCGGUCGCGGGACUCAUCGGGACACCAACAGCGACUGGGAUCGUCAUCCUGGCUAGUCCGCGUGACCAGGAGAUCCGAGCAGAGGCAUAGCGGACUGCGCACGGAGAAGUCUUGAGGGAAUGCCGUUG SEQ ID NO209星狀諾卡氏菌(Nocardia asteroides)ssrA，內(nèi)部部分ACTGTGTGCGCCGAGGTAGGGGAAGCGTGTCGGTGCAGGCTGGAGACCACCGTTAAGCGTCGCGGCAACCAATTAAGCGCCGATTCCAATCAGCGCGACTACGCCCTCGCTGCCTGATCAGCGACGGCTAGCTGTCGGCCCGGGTTGTGTTCCCGAACCCGGATGCCGGCATCATCTCAGGGAACTCACCGTGTTCGCCGGTCGCGGACGGACACGGGACAGCAAACAGCGACTGGGATCGTCATCTCGGCTTGTTCGCGTGACCGGGAGATCCAAGTAGAGACATAGCGGACTGCACACGGAGAAGCCCTACTGACTCGACACAG SEQ ID NO210星狀諾卡氏菌(Nocardia asteroides)tmRNA，內(nèi)部部分ACUGUGUGCGCCGAGGUAGGGGAAGCGUGUCGGUGCAGGCUGGAGACCACCGUUAAGCGUCGCGGCAACCAAUUAAGCGCCGAUUCCAAUCAGCGCGACUACGCCCUCGCUGCCUGAUCAGCGACGGCUAGCUGUCGGCCCGGGUUGUGUUCCCGAACCCGGAUGCCGGCAUCAUCUCAGGGAACUCACCGUGUUCGCCGGUCGCGGACGGACACGGGACAGCAAACAGCGACUGGGAUCGUCAUCUCGGCUUGUUCGCGUGACCGGGAGAUCCAAGUAGAGACAUAGCGGCUGCACACGGAGAAGCCCUACUGACUCGACACAG SEQ ID NO211腸炎沙門氏菌(Salmonella enteritidis)ssrA，內(nèi)部部分ACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAAATAGTCGCAAACGACGAAACCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGATGCCCTGCCTGGGGTTGAAGCGTTAAAACGAATCAGGCTAGTCTGGTAGTGGCGTGTCCGTCCGCAGGTGCCAGGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGG SEQ ID NO212腸炎沙門氏菌(Salmonella enteritidis)tmRNA，內(nèi)部部分ACGGGAUUUGCGAAACCCAAGGUGCAUGCCGAGGGGCGGUUGGCCUCGUAAAAAGCCGCAAAAAAAUAGUCGCAAACGACGAAACCUACGCUUUAGCAGCUUAAUAACCUGCUUAGAGCCCUCUCUCCCUAGCCUCCGCUCUUAGGACGGGGAUCAAGAGAGGUCAAACCCAAAAGAGAUCGCGUGGAUGCCCUGCCUGGGGUUGAAGCGUUAAAACGAAUCAGGCUAGUCUGGUAGUGGCGUGUCCGUCCGCAGGUGCCAGGCGAAUGUAAAGACUGACUAAGCAUGUAGUACCGAGGAUGUAGGAAUUUCGG SEQ ID No213表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)ssrA，內(nèi)部部分ACAGGGGTCCCCCGAGCTTATTAAGCGTGTCGGAGGGTTGGCTCCGTCATCAACACATTTCGGTTAAATATAACTGACAAATCAAACAATAATTTCGCAGTAGCTGCGTAATAGCCACTGCATCGCCTAACAGCATCTCCTACGTGCTGTTAACGCGATTCAACCCTAGTAGGATATGCTAAACACTGCCGCTTGAAGTCTGTTTAGATGAAATATAATCAAGCTAGTATCATGTTGGTTGTTTATTGCTTAGCATGATGCGAAAATTATCAATAAACTACACACGTAGAAAGATTTGTATCAGGACCTCTGGSEQ ID NO214表皮葡萄球菌(Staphylococcus epidermidis)(NCTC 11047)tmRNA，內(nèi)部部分ACAGGGGUCCCCCGAGCUUAUUAAGCGUGUCGGAGGGUUGGCUCCGUCAUCAACACAUUUCGGUUAAAUAUAACUGACAAAUCAAACAAUAAUUUCGCAGUAGCUGCGUAAUAGCCACUGCAUCGCCUAACAGCAUCUCCUACGUGCUGUUAACGCGAUUCAACCCUAGUAGGAUAUGCUAAACACUGCCGCUUGAAGUCUGUUUAGAUGAAAUAUAAUCAAGCUAGUAUCAUGUUGGUUGUUUAUUGCUUAGCAUGAUGCGAAAAUUAUCAAUAAACUACACACGUAGAAAGAUUUGUAUCAGGACCUCUGG SEQ ID NO215無乳鏈球菌(Streptococcus agalactiae)(NCTC 8181)ssrA，內(nèi)部部分ACAGGCATTATGAGGTATATTTTGCGACTCATCGGCAGATGTAAAATGCCAGTTAAATATAACTGCAAAAAATACAAATTCTTACGCATTAGCTGCCTAAAAAACAGCCTGCGTGATCTTCACAAGATTGTTTGCGTTTTGCTAGAAGGTCTTATTTATCAGCAAACTACGTTTGGCTACTGTCTAGTTAGTTAAAAAGAGATTTATAGACTCGCTATGTGAGGGCTTGAGTTATGTGTCATCACCTAGTTAAATCAATACATAACCTATAGTTGTAGACAAATATATTAGCAGATGTTTGG SEQ ID NO216無乳鏈球菌(Streptococcus agalactiae)(NCTC 8181)tmRNA，內(nèi)部部分ACAGGCAUUAUGAGGUAUAUUUUGCGACUCAUCGGCAGAUGUAAAAUGCCAGUUAAAUAUAACUGCAAAAAAUACAAAUUCUUACGCAUUAGCUGCCUAAAAAACAGCCUGCGUGAUCUUCACAAGAUUGUUUGCGUUUUGCUAGAAGGUCUUAUUUAUCAGCAAACUACGUUUGGCUACUGUCUAGUUAGUUAAAAAGAGAUUUAUAGACUCGCUAUGUGAGGGCUUGAGUUAUGUGUCAUCACCUAGUUAAAUCAAUACAUAACCUAUAGUUGUAGACAAAUAUAUUAGCAGAUGUUUGG SEQ ID NO217上面所提到的序列SEQ ID NO47-62，65-68，71和72，98和99，159-168和176-217為新序列。
上面所提到的序列能夠用于形成ssrA基因序列的數(shù)據(jù)庫，可用來鑒定細菌種類或產(chǎn)生核酸診斷檢驗盒。
下面為與本發(fā)明相應(yīng)的代表性探針沙門氏菌1)屬特異性探針5’-CGAATCAGGCTAGTCTGGTAG-3’ SEQ ID NO218分枝桿菌2)用于檢測肺結(jié)核復(fù)合物的寡聚核苷酸探針TB01 5’-ACTCCTCGGACA(A/G)CCACAGCGA-3’SEQ ID NO2193)用于檢測鳥分枝桿菌和鳥分枝桿菌(副結(jié)核亞種)序列的寡核苷酸探針探針1PAV1-5’-GTTGCAAATAGATAAGCGCC-3’SEQ IDNO220探針2PAV2-5’-TCCGTCAGCCCGGGAACGCC-3’SEQID NO221李斯特氏菌4)用于測定熱殺滅處理細胞后tmRNA完整性的寡核苷酸探針LVtm5′-TTTTGTTTTTCTTTGCCA-3′SEQ ID NO222大腸桿菌5)用于測定熱殺死處理細胞后tmRNA完整性的寡核苷酸探針Evtm5′-AGTTTTCGTCGTTTGCGA-3′SEQ ID NO223
下面為根據(jù)本發(fā)明的具代表性的引物分枝桿菌1)用于擴增所有分枝桿菌序列的簡并引物5′引物10SAAM3-5′-CAGGCAA(G/C)(A/T/C)GACCACCGTAA-3′SEQ ID NO2243′引物10SAAM4-5′GGATCTCC(C/T)G(A/G)TC(A/T)C(A/G)CG(A/G)AC(A/T)A-3′SEQ ID NO2252)用于檢測鳥分枝桿菌和鳥分枝桿菌(副結(jié)核亞種)序列的寡核苷酸引物5’引物AP1正向-5’-TGCCGGTGCAGGCAACTG-3’SEQ ID NO2263’引物AP2反向-5’-CACGCGAACAAGCCAGGA-3’SEQ IDNO227附圖簡述在附圖中

圖1為大腸桿菌和霍亂弧菌ssrA基因序列的Clustal對比；圖2為從大腸桿菌和霍亂弧菌細胞中制備的全細胞RNA的瓊脂糖凝膠照片；圖3為霍亂弧菌寡核苷酸探針與大腸桿菌和霍亂弧菌的tmRNA的轉(zhuǎn)錄序列雜交的放射自顯影照片；圖4為用通用ssrA基因擴增引物從一系列生物體中得到的擴增產(chǎn)物的瓊脂糖凝膠照片；圖5為李斯特氏菌ssrA基因序列的Clustal對比；圖6為單核細胞增多性李斯特氏菌和枯草芽孢桿菌ssrA/tmRNA基因序列的Clustal對比；圖7為用李斯特菌屬特異性PCR擴增引物從一系列生物體中得到的擴增產(chǎn)物的瓊脂糖凝膠照片；圖8為李斯特菌屬特異性寡核苷酸探針與實施例4中制備的一系列生物體雜交的放射自顯影照片；圖9為單核細胞增多性李斯特氏菌種特異性寡核苷酸探針與實施例7中制備的一系列生物體雜交的放射自顯影照片；圖10為如實施例6中所述的用多色探針檢測方法檢測尼龍膜上李斯特氏菌ssrA基因的計算機掃描圖象；圖11為沙眼衣原體菌株的ssrA基因序列的Clustal對比；圖12為幽門螺桿菌菌株ssrA基因序列的Clustal對比；圖13為生殖道枝原體菌株ssrA基因序列的Clustal對比；圖14為淋病奈瑟球菌菌株ssrA基因序列的Clustal對比；圖15為單核細胞增多性李斯特氏菌菌株ssrA基因序列的Clustal對比；圖16為單核細胞增多性李斯特氏菌和無害李斯特菌菌株的ssrA基因序列的Clustal對比圖17為李斯特菌寡聚核苷酸探針(Evtm)與經(jīng)中度熱處理的大腸桿菌細胞中分離的全RNA樣品雜交的放射自顯影照片；圖18為李斯特菌寡聚核苷酸探針(Evtm)與經(jīng)極度熱處理的大腸桿菌細胞中分離的全RNA樣品雜交的放射自顯影照片；圖19為李斯特氏菌寡聚核苷酸探針(Lvtm)與經(jīng)中度熱處理的單核細胞增多性李斯特氏菌細胞中分離的全RNA樣品雜交的放射自顯影照片；圖20為李斯特氏菌寡聚核苷酸探針(Lvtm)與經(jīng)極度熱處理的單核細胞增多性李斯特氏菌細胞中分離的全RNA樣品雜交的放射自顯影照片；圖21為經(jīng)熱處理后不同時間點tmRNA的RT-PCR產(chǎn)物的瓊脂糖凝膠照片。
本發(fā)明將通過下面的實施例作進一步的闡明。完成本發(fā)明的方式實施例1檢查可用的tmRNA序列的初級核苷酸序列用Clustal W核酸序列對比程序?qū)捎玫膖mRNA進行比較性初級核酸序列對比，表明原核生物的tmRNA比其它細菌高拷貝數(shù)RNA具有更顯著程度的核酸序列變異性和非同源性，如表1所示；表1不同細菌的RNA分子間核苷酸序列同源性百分比

這些不同細菌的tmRNA序列間的非同源性區(qū)位于分子的中間，tmRNA分子內(nèi)核苷酸序列的非同源性程度表明可制備屬和種特異性探針以用來區(qū)別和/或檢測細菌。
核苷酸序列對比已經(jīng)表明tmRNA分子的5’和3’側(cè)翼區(qū)都有種內(nèi)和屬內(nèi)的高度同源性。這表明可制備通用寡核苷酸引物，以用來從多種細菌中擴增ssrA基因或其編碼的tmRNA。
我們已經(jīng)證明來源于不同生物體的tmRNA分子的核苷酸序列內(nèi)的同源區(qū)和非同源區(qū)可以被用來作為在分子水平鑒定和檢測生物體的基礎(chǔ)。實施例2霍亂弧菌tmRNA特異性探針的設(shè)計如圖1所顯示的大腸桿菌核苷酸序列(SEQ ID NO.37)和霍亂弧菌ssrA序列(SEQ ID NO.127)的對比表明這兩種細菌在系統(tǒng)發(fā)生上有緊密的聯(lián)系。但是這些序列間的非同源區(qū)由缺乏星號標明。合成了圖1中加下劃線的霍亂弧菌ssrA核苷酸序列可變區(qū)的互補的寡核苷酸探針。
霍亂弧菌tmRNA特異性探針的序列為5’-AACGAATGGCTAACCTGAA-3’SEQ ID NO.169在大腸桿菌和霍亂弧菌生長的中間指數(shù)期和穩(wěn)定期，從液體培養(yǎng)物中分離得到全RNA。如圖2所示等量的被分離的全RNA在變性甲醛瓊脂糖凝膠中電泳并被印跡到HYBOND-N尼龍膜上，其中泳道1-4分別代表泳道1中間指數(shù)期大腸桿菌全RNA泳道2中間指數(shù)期霍亂弧菌全RNA泳道3穩(wěn)定期大腸桿菌全RNA泳道4穩(wěn)定期霍亂弧菌全RNA將所得的Northern印跡與末端標記γP32的霍亂弧菌tmRNA特異性探針雜交。如圖3所示的雜交試驗的結(jié)果證明了探針的特異性，因為只有霍亂弧菌tmRNA被檢測到。而且，觀察到在生長穩(wěn)定期的培養(yǎng)液中分離的霍亂弧菌tmRNA有更大程度的雜交信號強度，這表明在這一時期在霍亂弧菌細胞中存在更大拷貝數(shù)目的tmRNA分子。實施例3用于表征未知ssrA基因和tmRNA序列的通用ssrA/tmRNA寡核苷酸擴增引物的產(chǎn)生所有可用的ssrA基因和tmRNA序列的Clustal W對比表明能夠設(shè)計出簡并的寡核苷酸引物，用來從多種生物體中擴增ssrA基因和tmRNA核苷酸序列。
可合成簡并的寡核苷酸引物，用來從大范圍的細菌的全基因組DNA制備物中PCR擴增得到ssrA基因序列。
合成的簡并寡核苷酸如下所示(a)tmU5’5’體外PCR擴增引物5’-GGG(A/C)(C/T)TACGG(A/T)TTCGAC-3’SEQ ID NO170(b)tmU5’3’體外PCR擴增引物5’-GGGA(A/G)TCGAACC(A/G)(C/G)GTCC-3’SEQ ID NO171括號內(nèi)為簡并堿基位置。
PCR反應(yīng)的產(chǎn)物在瓊脂糖凝膠中電泳，如圖4所示在所有例子中都擴增得到約350堿基對PCR產(chǎn)物，這表明簡并tmRNA引物的通用性。
圖4中的各泳道分別代表A分子量標記V1大腸桿菌2沙門氏菌浦納亞種3產(chǎn)氣克雷伯氏菌4奇異變形菌5雷氏變形菌6氣單胞菌7金黃色葡萄球菌8腸球菌模式種(糞鏈球菌)9乳桿菌模式種10枯草芽孢桿菌11單核細胞增多性李斯特氏菌12無害李斯特菌13墨氏李斯特菌14威氏李斯特菌15格氏李斯特菌16牛分枝桿菌B分子量標記V
如表2所示，所述通用引物從革蘭氏陽性和革蘭氏陰性細菌中均擴增到了ssrA基因。
表2用通用擴增引物進行細菌物種檢測

實施例4分離和表征以前未知的細菌ssrA/tmRNA核苷酸序列由實施例2，從李斯特菌和牛分枝桿菌的基因組DNA中擴增得到的PCR產(chǎn)物被亞克隆到T末端質(zhì)粒載體來進行DNA測序。每個物種挑選3個重組克隆并用雙脫氧測序法進行測序。對每個亞克隆的DNA雙鏈的序列進行測定。
牛分枝桿菌ssrA基因的核苷酸序列與肺結(jié)核分枝桿菌ssrA基因序列有100％同源性。
圖5所示為從李斯特菌得到的新ssrA基因序列(SEQ ID NOS 51，53，55，59和61)的Clustal W對比。該分析表明能夠產(chǎn)生用于李斯特菌的屬特異性探針和寡核苷酸擴增引物。而且，該對比還表明可產(chǎn)生種特異性寡核苷酸探針，并能夠從其它李斯特菌中區(qū)分出單核細胞增多性李斯特氏菌。
在圖5中，據(jù)推測的屬特異性寡核苷酸引物L(fēng)tm1和Ltm2被框出，同時作為屬特異性李斯特菌寡核苷酸探針的LGtM也被框出。據(jù)推測的單核細胞增多性李斯特氏菌種特異性寡核苷酸探針序列以下劃線和斜體標識。
為進一步說明ssrA基因/tmRNA核酸靶點為適合細菌診斷的靶點，進行了單核細胞增多性李斯特氏菌ssrA基因核苷酸序列(SEQ IDNO.55)和枯草芽孢桿菌ssrA基因核苷酸序列(SEQ ID NO.11)(一種和李斯特氏菌在種系發(fā)生上密切相關(guān)的細菌)的比較性對比，如圖6所示。對該序列對比進行序列分析表明其序列同源性為41％，相應(yīng)的16S rRNA對比表明核苷酸序列有87％的同源性(數(shù)據(jù)沒有出示)。實施例5用于李斯特氏菌菌種的ssrA基因/tmRNA屬特異性擴增引物，屬特異性和種特異性探針的產(chǎn)生和應(yīng)用用實施例4中李斯特氏菌的ssrA基因/tmRNA核苷酸序列對比，分析ssrA基因/tmRNA核苷酸序列區(qū)域以決定其是否適用于產(chǎn)生屬特異性擴增引物和屬特異性及種特異性寡核苷酸探針。在分析中，表現(xiàn)出與枯草芽孢桿菌有最大程度差異的區(qū)域被選出來設(shè)計這些擴增引物和探針。
下面為合成的寡核苷酸的序列(a)Ltm15’李斯特氏菌屬特異性擴增引物5’-AAAGCCAATAATAACTGG-3’SEQ ID NO172
(b)Ltm23’李斯特氏菌屬特異性擴增引物5’-CCAGAAATATCGGCACTT-3’SEQ ID NO173(c)LGtm李斯特氏菌屬特異性雜交探針5’-GTGAGACCCTTACCGTAG-3’SEQ ID NO174(d)LStm單核細胞增多性李斯特氏菌種特異性雜交探針5’-TCTATTTAACCCCAGACG-3’SEQ ID NO175屬特異性擴增引物L(fēng)tm1和Ltm2用于與做為模板的二十個不同菌株的全基因組DNA進行一系列PCR反應(yīng)。用這些引物只能從李斯特氏菌中擴增得到ssrA基因序列(260堿基對產(chǎn)物)(圖7和表3)，這表明ssrA基因/tmRNA適合于作為體外擴增某細菌屬的靶點。沒有檢測到其它細菌的擴增產(chǎn)物，盡管采用如實施例2所描述的通用引物(tmU5’和tmU3’)從這些細菌的DNA中PCR擴增得到了PCR產(chǎn)物。
圖7中的各泳道分別代表A分子量標記V1大腸桿菌2沙門氏菌浦納亞種3產(chǎn)氣克雷伯氏菌4奇異變形菌5雷氏變形菌6氣單胞菌7金黃色葡萄球菌8腸球菌模式種9乳桿菌模式種10枯草芽孢桿菌11單核細胞增多性李斯特氏菌 菌株112單核細胞增多性李斯特氏菌 菌株213單核細胞增多性李斯特氏菌 菌株3
14單核細胞增多性李斯特氏菌 菌株415單核細胞增多性李斯特氏菌 臨床分離物16無害李斯特菌17墨氏李斯特菌18威氏李斯特菌19格氏李斯特菌20牛分枝桿菌B分子量標記V表3用李斯特氏菌特異性擴增引物進行細菌種的檢測

如圖4所示，李斯特氏菌屬特異性寡核苷酸探針LGtM進行Southern印跡雜交。如圖8和表4所示，只在各李斯特氏菌種中觀察到陽性雜交信號，表明tmRNA序列可以作為檢測特定屬的靶點。
圖8中所示的各泳道為A分子量標記V1大腸桿菌2沙門氏菌浦納亞種3產(chǎn)氣克雷伯氏菌4奇異變形菌5雷氏變形菌6氣單胞菌7金黃色葡萄球菌8腸球菌模式種9乳桿菌模式種10枯草芽孢桿菌11單核細胞增多性李斯特氏菌12無害李斯特菌13墨氏李斯特菌14威氏李斯特菌15格氏李斯特菌16牛分枝桿菌B分子量標記V用本實施例所描述的屬特異性擴增得到的PCR產(chǎn)物，如圖7所示，進行Southern印跡，其與單核細胞增多性李斯特氏菌種特異性寡核苷酸探針雜交。如圖9和表4所示在四株單核細胞增多性李斯特氏菌中的三株和一株臨床分離得到的菌株檢測到陽性雜交信號。
圖9中的各泳道代表
A分子量標記V1大腸桿菌2沙門氏菌浦納亞種3產(chǎn)氣克雷伯氏菌4奇異變形菌5雷氏變形菌6氣單胞菌7金黃色葡萄球菌8腸球菌模式種9乳桿菌模式種10枯草芽孢桿菌11單核細胞增多性李斯特氏菌 菌株112單核細胞增多性李斯特氏菌 菌株213單核細胞增多性李斯特氏菌 菌株314單核細胞增多性李斯特氏菌 菌株415單核細胞增多性李斯特氏菌 臨床分離物16無害李斯特菌17墨氏李斯特菌18威氏李斯特菌19格氏李斯特菌20牛分枝桿菌B分子量標記V
表4李斯特氏菌屬特異性探針和單核細胞增多性李斯特氏菌種特異性探針的特異性

菌株4，一種典型的單核細胞增多性李斯特氏菌菌株，用這種探針不能產(chǎn)生陽性信號。對從該菌株中PCR擴增得到的ssrA基因進行DNA測序表明其含有與無害李斯特氏菌相同的探針靶點區(qū)。但是應(yīng)該注意到從該菌株得到的ssrA基因包含的其它區(qū)域的序列與前述的單核細胞增多性李斯特氏菌中的這些序列相同而不同于無害李斯特氏菌，如圖15所示。所以可合成針對這些可變區(qū)其中之一的種特異性寡核苷酸探針，該探針或在種(例如單核細胞增多性李斯特氏菌)內(nèi)識別每種株型(分離物)。實施例6李斯特氏菌ssrA基因序列的多色探針檢測將LGTm(A)，LStm(B)和烏普薩拉彎曲桿菌的16S-23S rRNA間隔序列(C-5′CATTAAACTTTAGCAAGGAAGTG 3′SEQ ID NO228)寡核苷酸探針非可逆地結(jié)合到尼龍膜上與擴增的ssrA PCR產(chǎn)物進行雜交，所述擴增利用屬特異性引物L(fēng)tm1 and Ltm2(Ltm1的5′端標記有生物素)，它們來源于單核細胞增多性李斯特氏菌(1-6)，無害李斯特菌(7-10)，伊氏李斯特氏菌(11)，墨氏李斯特氏菌(12)，斯氏李斯特氏菌(13)，威氏李斯特氏菌(14)和格氏李斯特氏菌(15)。用tmU5′和tmU3′(tmU5′的5′端標記有生物素)擴增得到的ssrA的PCR擴增產(chǎn)物也被雜交到尼龍膜條帶上，被檢測細菌來源于革蘭氏陽性菌枯草芽孢桿菌、乳桿菌模式種、金黃色葡萄球菌、表皮葡萄球菌、腸球菌模式種、產(chǎn)氣莢膜梭菌(16-21)和革蘭氏陰性菌大腸桿菌、腸炎沙門氏菌、雷氏變形菌、產(chǎn)氣克雷伯氏菌(22-25)。如圖10所示，經(jīng)雜交后，對生物素比色檢驗進行顯影表明條帶1-6表明ssrA的PCR擴增產(chǎn)物來自于單核細胞增多性李斯特氏菌并證明PCR擴增產(chǎn)物來自李斯特氏菌，因A和B都能夠進行顏色檢測；條帶7-15證明PCR產(chǎn)物來自于李斯特氏菌，但其中只有A能夠進行顏色檢測；條帶16-25表明PCR產(chǎn)物不是從李斯特氏菌中得到的，因沒有檢測到顏色。C是陰性寡核苷酸對照探針而D為對所有樣品的比色檢驗的陽性對照。實施例7用ssrA/tmRNA序列來區(qū)分生物體的物種圖11(SEQ ID NOS.19和21)，圖12(SEQ ID NOS.41和43)，圖13(SEQ ID NOS.77和79)，圖14(SEQ ID NOS.83和85)15和16(SEQ ID NO.53，55和57)所示的Clustal W序列對比表明在同一種細菌的不同菌株的ssrA/tmRNA序列內(nèi)存在著核苷酸的差異。這表明ssrA/tmRNA序列潛在地可以用來區(qū)分同一細菌種類中不同菌株的個體和/或集體。這有助于其應(yīng)用于流行病學(xué)研究和細菌種群分析。實施例8對細菌細胞進行中度和極度熱處理后分析tmRNA的完整性對培養(yǎng)物進行熱處理將大腸桿菌和單核細胞增多性李斯特氏菌在80℃進行熱處理，對于大腸桿菌而言處理20分鐘，對于單核細胞增多性李斯特氏菌處理40分鐘，以及于120℃處理15分鐘(高壓釜)。經(jīng)過過夜生長和熱處理后在0小時，1小時，2小時，6小時，12小時和48小時進行測驗。每個時期的檢測都沒觀察到存活的菌體。在這些時期全RNA被分離并在變性的1.2％瓊脂糖凝膠中電泳及進行Northern印跡。在大腸桿菌的例子中(圖17和圖18)每個印跡都會與放射性標記的寡核苷酸探針Evtm雜交，在單核細胞增多性李斯特氏菌例子中(圖19和圖20)印跡與放射性標記的LVtm雜交。在每個檢測樣品中沒有檢測到tmRNA轉(zhuǎn)錄序列，這表明熱處理后tmRNA轉(zhuǎn)錄序列已被降解。帶有符號+ve的泳道為全RNA樣品的陽性對照。實施例9用tmRNA轉(zhuǎn)錄序列來區(qū)別活的和死的細菌100ml單核細胞增多性李斯特氏菌在液體培養(yǎng)液中培養(yǎng)過夜。生長后，對細胞進行連續(xù)稀釋并通過在營養(yǎng)瓊脂平板上鋪板來測定存活率。同時，從這些細胞的1ml液體中分離得到全RNA。剩余的細胞在65℃熱處理20分鐘。接著將細胞除去來進行存活率分析和全RNA分離。在處理后0小時，2小時，6小時和24小時的時間點取樣品來測定存活率和RNA分離。
在營養(yǎng)瓊脂平板上鋪板表明熱處理能殺死單核細胞增多性李斯特氏菌細胞，因沒有觀察到活的集落形成單位。每個分離的RNA樣品用DNA酶處理以除去所含的任何DNA并且用李斯特氏菌屬特異性ssrA/tmRNA寡核苷酸引物L(fēng)tm1和Ltm2對全RNA樣品(100ng)進行反轉(zhuǎn)錄PCR擴增。擴增反應(yīng)的陰性對照包括引物、靶序列和Taq聚合酶，但沒有反轉(zhuǎn)錄酶。
擴增反應(yīng)的結(jié)果如圖12所示。
擴增的tmRNA的RT-PCR產(chǎn)物只能在沒有經(jīng)熱處理的RNA樣品中檢測到。所有其它樣品中沒有產(chǎn)生RT-PCR產(chǎn)物，這表明這些樣品中tmRNA分子在熱處理后死的細胞中已經(jīng)被降解。
圖21中的各泳道代表A分子量標記V；1RNA的PCR擴增(沒有熱處理過的細胞)反轉(zhuǎn)錄酶(RT)，+Taq聚合酶(TP)；2RNA的RT-PCR(沒有經(jīng)過熱處理的細胞)，+RT，+TP；3RNA的PCR擴增(熱處理后0小時)，-RT，+TP；4RNA的RT-PCR(熱處理后0小時)，+RT，+TP；5RNA的PCR擴增(熱處理后1小時)，-RT，+TP；6RNA的RT-PCR(熱處理后1小時)，+RT，+TP；7RNA的PCR擴增(熱處理后2小時)，-RT，+TP；8RNA的RT-PCR(熱處理后2小時)，+RT，+TP；9RNA的PCR擴增(熱處理后6小時)，-RT，+TP；10RNA的RT-PCR(熱處理后6小時)，+RT，+TP；11RNA的PCR擴增(熱處理后24小時)，-RT，+TP；12RNA的RT-PCR(熱處理后24小時)，+RT，+TP；B分子量標記序列表<110> 愛爾蘭公司(愛爾蘭生物研究)等人<120> 應(yīng)用于原核生物和真核生物的基于核酸探針的診斷檢驗<130> P00-37-PCT<140><141><150> PCT/IE 99/00043<151> 1999-05-14<160> 228<170> PatentIn Ver.2.0<210> 1<211> 366<212> DNA<213> Actinobacillus actinomycetemcomitans<400> 1ggggctgatt ctggattcga cgggattagc gaagcccgaa gtgcacgtcg aggtgcggta 60ggcctcgtaa ataaaccgca aaaaaatagt cgcaaacgac gaacaatacg ctttagcagc 120ttaataacct gcctttagcc ttcgctcccc agcttccgct cgtaagacgg ggataaagcg 180gagtcaaacc aaaacgagat cgtgtggaag ccaccgtttg aggatcgaag cattaaatta 240aatcaaagta gcttaattgt cgcgtgtccg tcagcaggat taagtgaatt taaagaccgg 300actaaacgtg tagtgctaac ggcagaggaa tttcggacgg gggttcaact ccccccagct 360ccacca366<210> 2<211> 366<212> RNA<213> Actinobacillus actinomycetemcomitans<400> 2ggggcugauu cuggauucga cgggauuagc gaagcccgaa gugcacgucg aggugcggua 60ggccucguaa auaaaccgca aaaaaauagu cgcaaacgac gaacaauacg cuuuagcagc 120uuaauaaccu gccuuuagcc uucgcucccc agcuuccgcu cguaagacgg ggauaaagcg 180gagucaaacc aaaacgagau cguguggaag ccaccguuug aggaucgaag cauuaaauua 240aaucaaagua gcuuaauugu cgcguguccg ucagcaggau uaagugaauu uaaagaccgg 300acuaaacgug uagugcuaac ggcagaggaa uuucggacgg ggguucaacu ccccccagcu 360ccacca366<210> 3<211> 315<212> DNA<213> Aeromonas salmonicida<400> 3aagattcacg aaacccaagg tgcatgccga ggtgcggtag gcctcgttaa caaaccgcaa 60aaaaatagtc gcaaacgacg aaaactacgc actagcagct taataacctg catagagccc 120ttctacccta gcttgcctgt gtcctaggga atcggaaggt catccttcac aggatcgtgt 180ggaagtcctg ctcggggcgg aagcattaaa accaatcgag ctagtcaatt cgtggcgtgt 240ctctccgcag cgggttggcg aatgtaaaga gtgactaagc atgtagtacc gaggatgtag 300taattttgga cgggg 315<210> 4<211> 315<212> RNA<213> Aeromonas salmonicida<400> 4aagauucacg aaacccaagg ugcaugccga ggugcgguag gccucguuaa caaaccgcaa 60aaaaauaguc gcaaacgacg aaaacuacgc acuagcagcu uaauaaccug cauagagccc 120uucuacccua gcuugccugu guccuaggga aucggaaggu cauccuucac aggaucgugu 180ggaaguccug cucggggcgg aagcauuaaa accaaucgag cuagucaauu cguggcgugu 240cucuccgcag cggguuggcg aauguaaaga gugacuaagc auguaguacc gaggauguag 300uaauuuugga cgggg 315<210> 5<211> 349<212> DNA<213> Alcaligenes eutrophus<400> 5tgggccgacc tggtttcgac gtggttacaa agcagtgagg cataccgagg acccgtcacc 60tcgttaatca atggaatgca ataactgcta acgacgaacg ttacgcactc gcttaattgc 120ggccgtcctc gcactggctc gctgacgggc tagggtcgca agaccacgcg aggtatttac 180gtcagataag ctccggaagg gtcacgaagc cggggacgaa aacctagtga ctcgccgtcg 240tagagcgtgt tcgtccgatg cgccggttaa atcaaatgac agaactaagt atgtagaact 300ctctgtggag ggcttacgga cgcgggttcg attcccgccg gctccacca 349<210> 6<211> 349<212> RNA<213> Alcaligenes eutrophus<400> 6ugggccgacc ugguuucgac gugguuacaa agcagugagg cauaccgagg acccgucacc 60ucguuaauca auggaaugca auaacugcua acgacgaacg uuacgcacuc gcuuaauugc 120ggccguccuc gcacuggcuc gcugacgggc uagggucgca agaccacgcg agguauuuac 180gucagauaag cuccggaagg gucacgaagc cggggacgaa aaccuaguga cucgccgucg 240uagagcgugu ucguccgaug cgccgguuaa aucaaaugac agaacuaagu auguagaacu 300cucuguggag ggcuuacgga cgcggguucg auucccgccg gcuccacca 349<210> 7<211> 347<212> DNA<213> Aquifex aeolicus<400> 7gggggcggaa aggattcgac ggggacaggc ggtccccgag gagcaggccg ggtggctccc 60gtaacagccg ctaaaacagc tcccgaagct gaactcgctc tcgctgccta attaaacggc 120agcgcgtccc cggtaggttt gcgggtggcc taccggaggg cgtcagagac acccgctcgg 180gctactcggt cgcacggggc tgagtagctg acacctaacc cgtgctaccc tcggggagct 240tgcccgtggg cgacccgagg ggaaatcctg aacacgggct aagcctgtag agcctcggat 300gtggccgccg tcctcggacg cgggttcgat tcccgccgcc tccacca 347<210> 8<211> 347<212> RNA<213> Aquifex aeolicus<400> 8gggggcggaa aggauucgac ggggacaggc gguccccgag gagcaggccg gguggcuccc 60guaacagccg cuaaaacagc ucccgaagcu gaacucgcuc ucgcugccua auuaaacggc 120agcgcguccc cgguagguuu gcggguggcc uaccggaggg cgucagagac acccgcucgg 180gcuacucggu cgcacggggc ugaguagcug acaccuaacc cgugcuaccc ucggggagcu 240ugcccguggg cgacccgagg ggaaauccug aacacgggcu aagccuguag agccucggau 300guggccgccg uccucggacg cggguucgau ucccgccgcc uccacca 347<210> 9<211> 316<212> DNA<213> Bacillus megaterium<400> 9agggtagttc gagcttaggt tgcgagtcga ggagatggcc tcgttaaaac atcaacgcca 60ataataactg gcaaatctaa caataacttc gctttagctg cataatagta gcttagcgtt 120cctccctcca tcgcccatgt ggtagggtaa gggactcact ttaagtgggc tacgccggag 180ttcgccgtct gaggacgaag gaagagaata atcagactag cgactgggac gcctgttggt 240aggcagaaca gctcgcgaat gatcaatatg ccaactacac tcgtagacgc ttaagtggcc 300atatttctgg acgtgg 316<210> 10<211> 316<212> RNA<213> Bacillus megaterium<400> 10aggguaguuc gagcuuaggu ugcgagucga ggagauggcc ucguuaaaac aucaacgcca 60auaauaacug gcaaaucuaa caauaacuuc gcuuuagcug cauaauagua gcuuagcguu 120ccucccucca ucgcccaugu gguaggguaa gggacucacu uuaagugggc uacgccggag 180uucgccgucu gaggacgaag gaagagaaua aucagacuag cgacugggac gccuguuggu 240aggcagaaca gcucgcgaau gaucaauaug ccaacuacac ucguagacgc uuaaguggcc 300auauuucugg acgugg 316<210> 11<211> 363<212> DNA<213> Bacillus subtilis<400> 11ggggacgtta cggattcgac agggatggat cgagcttgag ctgcgagccg agaggcgatc 60tcgtaaacac gcacttaaat ataactggca aaactaacag ttttaaccaa aacgtagcat 120tagctgccta ataagcgcag cgagctcttc ctgacattgc ctatgtgtct gtgaagagca 180catccaagta ggctacgctt gcgttcccgt ctgagaacgt aagaagagat gaacagacta 240gctctcggaa ggcccgcccg caggcaagaa gatgagtgaa accataaata tgcaggctac 300gctcgtagac gcttaagtaa tcgatgtttc tggacgtggg ttcgactccc accgtctcca 360cca 363<210> 12<211> 363<212> RNA<213> Bacillus subtilis<400> 12ggggacguua cggauucgac agggauggau cgagcuugag cugcgagccg agaggcgauc 60ucguaaacac gcacuuaaau auaacuggca aaacuaacag uuuuaaccaa aacguagcau 120uagcugccua auaagcgcag cgagcucuuc cugacauugc cuaugugucu gugaagagca 180cauccaagua ggcuacgcuu gcguucccgu cugagaacgu aagaagagau gaacagacua 240gcucucggaa ggcccgcccg caggcaagaa gaugagugaa accauaaaua ugcaggcuac 300gcucguagac gcuuaaguaa ucgauguuuc uggacguggg uucgacuccc accgucucca 360cca 363<210> 13<211> 387<212> DNA<213> Bordetella pertussis<400> 13ggggccgatc cggattcgac gtgggtcatg aaacagctca gggcatgccg agcaccagta 60agctcgttaa tccactggaa cactacaaac gccaacgacg agcgtctcgc tctcgccgct 120taagcggtga gccgctgcac tgatctgtcc ttgggtcagg cgggggaagg caacttcaca 180gggggcaacc ccgaaccgca gcagcgacat tcacaaggaa tcggccaccg ctggggtcac 240acggcgttgg tttaaattac gtgaatcgcc ctggtccggc ccgtcgatcg gctaagtcca 300gggttaaatc caaatagatc gactaagcat gtagaactgg ttgcggaggg cttgcggacg 360ggggttcaat tccccccggc tccacca 387<210> 14<211> 387<212> RNA<213> Bordetella pertussis<400> 14ggggccgauc cggauucgac gugggucaug aaacagcuca gggcaugccg agcaccagua 60agcucguuaa uccacuggaa cacuacaaac gccaacgacg agcgucucgc ucucgccgcu 120uaagcgguga gccgcugcac ugaucugucc uugggucagg cgggggaagg caacuucaca 180gggggcaacc ccgaaccgca gcagcgacau ucacaaggaa ucggccaccg cuggggucac 240acggcguugg uuuaaauuac gugaaucgcc cugguccggc ccgucgaucg gctaagucca 300ggguuaaauc caaauagauc gacuaagcau guagaacugg uugcggaggg cuugcggacg 360gggguucaau uccccccggc uccacca 387<210> 15<211> 362<212> DNA<213> Borrelia burgdorferi<400> 15ggggatgttt tggatttgac tgaaaatgtt aatattgtaa gttgcaggca gagggaatct 60cttaaaactt ctaaaataaa tgcaaaaaat aataacttta caagctcaaa tcttgtaatg 120gctgcttaag ttagcagagg gttttgttga atttggcttt gaggttcact tatactcttt 180tcgacatcaa agcttgctta aaaatgtttt caagttgatt tttagggact tttatacttg 240agagcaattt ggtggtttgc tagtatttcc aaaccatatt gcttaataaa atactagata 300agcttgtaga agcttatagt attattttta ggacgcgggt tcaattcccg ccatctccac 360ca362<210> 16<211> 362<212> RNA<213> Borrelia burgdorferi<400> 16ggggauguuu uggauuugac ugaaaauguu aauauuguaa guugcaggca gagggaaucu 60cuuaaaacuu cuaaaauaaa ugcaaaaaau aauaacuuua caagcucaaa ucuuguaaug 120gcugcuuaag uuagcagagg guuuuguuga auuuggcuuu gagguucacu uauacucuuu 180ucgacaucaa agcuugcuua aaaauguuuu caaguugauu uuuagggacu uuuauacuug 240agagcaauuu ggugguuugc uaguauuucc aaaccauauu gcuuaauaaa auacuagaua 300agcuuguaga agcuuauagu auuauuuuua ggacgcgggu ucaauucccg ccaucuccac 360ca362<210> 17<211> 359<212> DNA<213> Campylobacter jejuni<400> 17gggagcgact tggcttcgac aggagtaagt ctgcttagat ggcatgtcgc tttgggcaaa 60gcgtaaaaag cccaaataaa attaaacgca aacaacgtta aattcgctcc tgcttacgct 120aaagctgcgt aagttcagtt gagcctgaaa tttaagtcat actatctagc ttaattttcg 180gtcatttttg atagtgtagc cttgcgtttg acaagcgttg aggtgaaata aagtcttagc 240cttgcttttg agttttggaa gatgagcgaa gtagggtgaa gtagtcatct ttgctaagca 300tgtagaggtc tttgtgggat tatttttgga caggggttcg attcccctcg cttccacca 359<210> 18<211> 359<212> RNA<213> Campylobacter jejuni<400> 18gggagcgacu uggcuucgac aggaguaagu cugcuuagau ggcaugucgc uuugggcaaa 60gcguaaaaag cccaaauaaa auuaaacgca aacaacguua aauucgcucc ugcuuacgcu 120aaagcugcgu aaguucaguu gagccugaaa uuuaagucau acuaucuagc uuaauuuucg 180gucauuuuug auaguguagc cuugcguuug acaagcguug aggugaaaua aagucuuagc 240cuugcuuuug aguuuuggaa gaugagcgaa guagggugaa guagucaucu uugcuaagca 300uguagagguc uuugugggau uauuuuugga cagggguucg auuccccucg cuuccacca 359<210> 19<211> 420<212> DNA<213> Chlamydia trachomatis (D/UW-3/CX)<400> 19gggggtgtaa aggtttcgac ttagaaatga agcgttaatt gcatgcggag ggcgttggct 60ggcctcctaa aaagccgaca aaacaataaa tgccgaacct aaggctgaat gcgaaattat 120cagcttcgct gatctcgaag atctaagagt agctgcttaa ttagcaaagt tgttacctaa 180atacgggtga cccggtgttc gcgagctcca ccagaggttt tcgaaacacc gtcatgtatc 240tggttagaac ttaggtcctt taattctcga ggaaatgagt ttgaaattta atgagagtcg 300ttagtctcta taggggtttc tagctgagga gacataacgt atagtaccta ggaactaagc 360atgtagaggt tagcggggag tttactaagg acgagagttc gactctctcc acctccacca 420<210> 20<211> 420<212> RNA<213> Chlamydia trachomatis (D/UW-3/CX)<400> 20ggggguguaa agguuucgac uuagaaauga agcguuaauu gcaugcggag ggcguuggcu 60ggccuccuaa aaagccgaca aaacaauaaa ugccgaaccu aaggcugaau gcgaaauuau 120cagcuucgcu gaucucgaag aucuaagagu agcugcuuaa uuagcaaagu uguuaccuaa 180auacggguga cccgguguuc gcgagcucca ccagagguuu ucgaaacacc gucauguauc 240ugguuagaac uuagguccuu uaauucucga ggaaaugagu uugaaauuua augagagucg 300uuagucucua uagggguuuc uagcugagga gacauaacgu auaguaccua ggaacuaagc 360auguagaggu uagcggggag uuuacuaagg acgagaguuc gacucucucc accuccacca 420<210> 21<211> 421<212> DNA<213> Chlamydia trachomatis(小鼠肺炎)<400> 21gggggtgtaa aggtttcgac ttagaaatga agcgttaatt gcatgcggag ggcgttggct 60ggcctcctaa aaagccgaca aaacaataaa tgccgaacct aaggctgaat gcgaaattat 120cagcttcgct gatcttaatg atctaagagt tgctgcttaa ttagcaaagt tgttacctaa 180gtactggtaa cccggtgttc gcgagctcca ccagaggttt tcgaaacgcc gtcatttatc 240tggttagaat tagggccttt taactctcaa gggaactaat ttgaatttta atgagagtcg 300ttggtctcta tagaggtttc tagctgagga gatataacgt aaaatattct agaaactaag 360catgtagagg ttagcgggga gtttactaag gacgagagtt cgaatctctc cacctccacc 420a 421<210> 22<211> 421<212> RNA<213> Chlamydia trachomatis(小鼠肺炎)<400> 22ggggguguaa agguuucgac uuagaaauga agcguuaauu gcaugcggag ggcguuggcu 60ggccuccuaa aaagccgaca aaacaauaaa ugccgaaccu aaggcugaau gcgaaauuau 120cagcuucgcu gaucuuaaug aucuaagagu ugcugcuuaa uuagcaaagu uguuaccuaa 180guacugguaa cccgguguuc gcgagcucca ccagagguuu ucgaaacgcc gucauuuauc 240ugguuagaau uagggccuuu uaacucucaa gggaacuaau uugaauuuua augagagucg 300uuggucucua uagagguuuc uagcugagga gauauaacgu aaaauauucu agaaacuaag 360cauguagagg uuagcgggga guuuacuaag gacgagaguu cgaaucucuc caccuccacc 420a 421<210> 23<211> 404<212> DNA<213> Chlorobium tepidum<400> 23ggggatgaca ggctatcgac aggataggtg tgagatgtcg ttgcactccg agtttcagca 60tggacggact cgttaaacaa gtctatgtac caatagatgc agacgattat tcgtatgcaa 120tggctgcctg attagcacaa gttaattcag aagccatcgt cctgcggtga atgcgcttac 180tctgaagccg ccggatggca taacccgcgc ttgagcctac gggttcgcgc aagtaagctc 240cgtacattca tgcccgaggg ggtgtgcggg taaccaatcg ggataagggg acgaacgctg 300ctggcggtgt aatcggacca cgaaaaacca accaccagag atgagtgtgg taactgcatc 360gagcagtgtc ctggacgcgg gttcaagtcc cgccatctcc acca 404<210> 24<211> 404<212> RNA<213> Chlorobium tepidum<400> 24ggggaugaca ggcuaucgac aggauaggug ugagaugucg uugcacuccg aguuucagca 60uggacggacu cguuaaacaa gucuauguac caauagaugc agacgauuau ucguaugcaa 120uggcugccug auuagcacaa guuaauucag aagccaucgu ccugcgguga augcgcuuac 180ucugaagccg ccggauggca uaacccgcgc uugagccuac ggguucgcgc aaguaagcuc 240cguacauuca ugcccgaggg ggugugcggg uaaccaaucg ggauaagggg acgaacgcug 300cuggcggugu aaucggacca cgaaaaacca accaccagag augagugugg uaacugcauc 360gagcaguguc cuggacgcgg guucaagucc cgccaucucc acca 404<210> 25<211> 294<212> DNA<213> Cyanophora paradoxa(藻類)藍色小體<400> 25ggggctgttt aggtttcgac gtttttttct aattatgttt gttaagcaag tcgaggattt 60gttctatctc gaaaatcaag aactctcaaa atttaaacgc aactaatatt gtacgtttta 120accgtaaagc agctttcgct gtttaataat tacttttaat ttaaaaacct aattttttta 180ggaatttatt tatttattgt ttatcctgct taatgaatta aaaaaagcta tacttgtgaa 240taaacgcata atttaaaaaa acggacgtgg gttcaaatcc caccagctcc acca 294<210> 26<211> 294<212> RNA<213> Cyanophora paradoxa(藻類)藍色小體<400> 26ggggcuguuu agguuucgac guuuuuuucu aauuauguuu guuaagcaag ucgaggauuu 60guucuaucuc gaaaaucaag aacucucaaa auuuaaacgc aacuaauauu guacguuuua 120accguaaagc agcuuucgcu guuuaauaau uacuuuuaau uuaaaaaccu aauuuuuuua 180ggaauuuauu uauuuauugu uuauccugcu uaaugaauua aaaaaagcua uacuugugaa 240uaaacgcaua auuuaaaaaa acggacgugg guucaaaucc caccagcucc acca 294<210> 27<211> 189<212> DNA<213> Clostridium acetobutylicum<400> 27aatctggcgt cgagagcggg gaaacgagcc ttacaaagct ttgagtaagg aacggaattt 60atgaagctac tgaagtgaaa agcttgtttg taggcgtttc atggagggaa tgttaaaata 120caaactgcac tcggagatgc ttaatgaaac cattttcgga caggggttcg attcccctcg 180cctccacca 189<210> 28<211> 189<212> RNA<213> Clostridium acetobutylicum<400> 28aaucuggcgu cgagagcggg gaaacgagcc uuacaaagcu uugaguaagg aacggaauuu 60augaagcuac ugaagugaaa agcuuguuug uaggcguuuc auggagggaa uguuaaaaua 120caaacugcac ucggagaugc uuaaugaaac cauuuucgga cagggguucg auuccccucg 180ccuccacca 189<210> 29<211> 349<212> DNA<213> Deinococcus radiodurans<400> 29gggggtgacc cggtttcgac aggggaactg aaggtgatgt tgcgtgtcga ggtgccgttg 60gcctcgtaaa caaacggcaa agccatttaa ctggcaacca gaactacgct ctcgctgctt 120aagtgagatg acgaccgtgc agcccggcct ttggcgtcgc ggaagtcact aaaaaagaag 180gctagcccag gcgattctcc atagccgacg gcgaaacttt atggagctac ggcctgcgag 240aacctgccca ctggtgagcg ccggcccgac aatcaaacag tgggatacac acgtagacgc 300acgctggacg gacctttgga cggcggttcg actccgccca cctccacca 349<210> 30<211> 349<212> RNA<213> Deinococcus radiodurans<400> 30gggggugacc cgguuucgac aggggaacug aaggugaugu ugcgugucga ggugccguug 60gccucguaaa caaacggcaa agccauuuaa cuggcaacca gaacuacgcu cucgcugcuu 120aagugagaug acgaccgugc agcccggccu uuggcgucgc ggaagucacu aaaaaagaag 180gcuagcccag gcgauucucc auagccgacg gcgaaacuuu auggagcuac ggccugcgag 240aaccugccca cuggugagcg ccggcccgac aaucaaacag ugggauacac acguagacgc 300acgcuggacg gaccuuugga cggcgguucg acuccgccca ccuccacca 349<210> 31<211> 330<212> DNA<213> Desulfovibrio desulfuricans<400> 31gggactggaa ccgtagcggc aggtcgaggc gccgctggcc tcgtaaaaag cggcacaaaa 60gtaattgcca acaacgatta cgactacgct tacgctgcct aataacagcg aggcaatgac 120cgtttaacgg tcgcgccgat cagggccatg cctgataacc ctgattggcg acacttatca 180ggctggcgaa aaccggctct cgccggggtt tttcgcgagg agtttaccgg cgggattgct 240gcgttgtgcc tggtcagggg ccaacagcgc ggtgaaatac atacttgacc taaacctgta 300atgcttcgtg tggaatgttc tcggacgggg 330<210> 32<211> 330<212> RNA<213> Desulfovibrio desulfuricans<400> 32gggacuggaa ccguagcggc aggucgaggc gccgcuggcc ucguaaaaag cggcacaaaa 60guaauugcca acaacgauua cgacuacgcu uacgcugccu aauaacagcg aggcaaugac 120cguuuaacgg ucgcgccgau cagggccaug ccugauaacc cugauuggcg acacuuauca 180ggcuggcgaa aaccggcucu cgccgggguu uuucgcgagg aguuuaccgg cgggauugcu 240gcguugugcc uggucagggg ccaacagcgc ggugaaauac auacuugacc uaaaccugua 300augcuucgug uggaauguuc ucggacgggg 330<210> 33<211> 318<212> DNA<213> Dichelobacter nodosus<400> 33ctcgaggtgc atgtcgagaa tgagagaatc tcgttaaata ctttcaaaac ttatagttgc 60aaacgacgac aactacgctt tagcggctta attcccgctt tcgcttacct agatttgtct 120gtgggtttac cgtaagcgac attaacacag aatcgctggt taacgcgtcc gctgttaatc 180ggttaaatta agcggaatcg cttgtaaaat gcctgagcgt tggctgttta tgagttaaac 240ctaattaact gctctaaaca tgtagtacca aaagttaagg attcgcggac gggggttcaa 300atccccccgc ctccacca 318<210> 34<211> 318<212> RNA<213> Dichelobacter nodosus<400> 34cucgaggugc augucgagaa ugagagaauc ucguuaaaua cuuucaaaac uuauaguugc 60aaacgacgac aacuacgcuu uagcggcuua auucccgcuu ucgcuuaccu agauuugucu 120guggguuuac cguaagcgac auuaacacag aaucgcuggu uaacgcgucc gcuguuaauc 180gguuaaauua agcggaaucg cuuguaaaau gccugagcgu uggcuguuua ugaguuaaac 240cuaauuaacu gcucuaaaca uguaguacca aaaguuaagg auucgcggac ggggguucaa 300auccccccgc cuccacca 318<210> 35<211> 367<212> DNA<213> Enterococcus faecalis<400> 35gggggcgtta cggattcgac aggcatagtt gagcttgaat tgcgtttcgt aggttacggc 60tacgttaaaa cgttacagtt aaatataact gctaaaaacg aaaacaattc tttcgcttta 120gctgcctaaa aaccagctag cgaagatcct cccggcatcg cccatgtgct cgggtcaggg 180tcctaatcga agtgggatac gctaaatttt tccgtctgta aaatttagag gagcttacca 240gactagcaat acagaatgcc tgtcactcgg cacgctgtaa agcgaacctt taaatgagtg 300tctatgaacg tagagattta agtggcaata tgtttggacg cgggttcgac tcccgccgtc 360tccacca 367<210> 36<211> 367<212> RNA<213> Enterococcus faecalis<400> 36gggggcguua cggauucgac aggcauaguu gagcuugaau ugcguuucgu agguuacggc 60uacguuaaaa cguuacaguu aaauauaacu gcuaaaaacg aaaacaauuc uuucgcuuua 120gcugccuaaa aaccagcuag cgaagauccu cccggcaucg cccaugugcu cgggucaggg 180uccuaaucga agugggauac gcuaaauuuu uccgucugua aaauuuagag gagcuuacca 240gacuagcaau acagaaugcc ugucacucgg cacgcuguaa agcgaaccuu uaaaugagug 300ucuaugaacg uagagauuua aguggcaaua uguuuggacg cggguucgac ucccgccguc 360uccacca 367<210> 37<211> 363<212> DNA<213> Escherichia coli<400> 37ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt 60ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt 120aataacctgc ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag 180gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat 240caggctagtt tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact 300aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca 360cca 363<210> 38<211> 363<212> RNA<213> Escherichia coli<400> 38ggggcugauu cuggauucga cgggauuugc gaaacccaag gugcaugccg aggggcgguu 60ggccucguaa aaagccgcaa aaaauagucg caaacgacga aaacuacgcu uuagcagcuu 120aauaaccugc uuagagcccu cucucccuag ccuccgcucu uaggacgggg aucaagagag 180gucaaaccca aaagagaucg cguggaagcc cugccugggg uugaagcguu aaaacuuaau 240caggcuaguu uguuaguggc guguccgucc gcagcuggca agcgaaugua aagacugacu 300aagcauguag uaccgaggau guaggaauuu cggacgcggg uucaacuccc gccagcucca 360cca 363<210> 39<211> 366<212> DNA<213> Haemophilus influenzae<400> 39ggggctgatt ctggattcga cgggattagc gaagcccaag gtgcacgtcg aggtgcggta 60ggcctcgtaa ataaaccgca aaaaaatagt cgcaaacgac gaacaatacg ctttagcagc 120ttaataacct gcatttagcc ttcgcgctcc agcttccgct cgtaagacgg ggataacgcg 180gagtcaaacc aaaacgagat cgtgtggaag ccaccgtttg aggatcgaag cactaaattg 240aatcaaacta gcttaagttt agcgtgtctg tccgcatgct taagtgaaat taaagacgag 300actaaacgtg tagtactgaa ggtagagtaa tttcggacgg gggttcaact ccccccagct 360ccacca366<210> 40<211> 366<212> RNA<213> Haemophilus influenzae<400> 40ggggcugauu cuggauucga cgggauuagc gaagcccaag gugcacgucg aggugcggua 60ggccucguaa auaaaccgca aaaaaauagu cgcaaacgac gaacaauacg cuuuagcagc 120uuaauaaccu gcauuuagcc uucgcgcucc agcuuccgcu cguaagacgg ggauaacgcg 180gagucaaacc aaaacgagau cguguggaag ccaccguuug aggaucgaag cacuaaauug 240aaucaaacua gcuuaaguuu agcgugucug uccgcaugcu uaagugaaau uaaagacgag 300acuaaacgug uaguacugaa gguagaguaa uuucggacgg ggguucaacu ccccccagcu 360ccacca366<210> 41<211> 340<212> DNA<213> Helicobacter pylori (ATC 43504)<400> 41agatttcttg tcgcgcagat agcatgccaa gcgctgcttg taaaacagca acaaaaataa 60ctgtaaacaa cacagattac gctccagctt acgctaaagc tgcgtgagtt aatctccttt 120tggagctgga ctgattagaa tttctagcgt tttaatcgct ccataacctt aagctagacg 180cttttaaaag gtggttcgcc ttttaaacta agaaacaaga actcttgaaa ctatcttaag 240gttttagaaa gttggaccag agctagtttt aaggctaaaa actaaccaat tttctaagca 300ttgtagaagt ttgtgtttag ggcaagattt ttggactggg 340<210> 42<211> 340<212> RNA<213> Helicobacter pylori (ATC 43504)<400> 42agauuucuug ucgcgcagau agcaugccaa gcgcugcuug uaaaacagca acaaaaauaa 60cuguaaacaa cacagauuac gcuccagcuu acgcuaaagc ugcgugaguu aaucuccuuu 120uggagcugga cugauuagaa uuucuagcgu uuuaaucgcu ccauaaccuu aagcuagacg 180cuuuuaaaag gugguucgcc uuuuaaacua agaaacaaga acucuugaaa cuaucuuaag 240guuuuagaaa guuggaccag agcuaguuuu aaggcuaaaa acuaaccaau uuucuaagca 300uuguagaagu uuguguuuag ggcaagauuu uuggacuggg 340<210> 43<211> 386<212> DNA<213> Helicobacter pylori(菌株 26695)<400> 43ggggctgact tggatttcga cagatttctt gtcgcacaga tagcatgcca agcgctgctt 60gtaaaacagc aacaaaaata actgtaaaca acacagatta cgctccagct tacgctaaag 120ctgcgtgagt taatctcctt ttggagctgg actgattaga atttctagcg ttttaatcgc 180tccataacct taagctagac gcttttaaaa ggtggttcgc cttttaaact aagaaacaag 240aactcttgaa actatctcaa ggttttagaa agttggacca gagctagttt taaggctaaa 300aaaccaacca attttctaag cattgtagaa gtttgtgttt agggcaagat ttttggactg 360gggttcgatt ccccacagct ccacca 386<210> 44<211> 386<212> RNA<213> Helicobacter pylori (菌株 26695)<400> 44ggggcugacu uggauuucga cagauuucuu gucgcacaga uagcaugcca agcgcugcuu 60guaaaacagc aacaaaaaua acuguaaaca acacagauua cgcuccagcu uacgcuaaag 120cugcgugagu uaaucuccuu uuggagcugg acugauuaga auuucuagcg uuuuaaucgc 180uccauaaccu uaagcuagac gcuuuuaaaa ggugguucgc cuuuuaaacu aagaaacaag 240aacucuugaa acuaucucaa gguuuuagaa aguuggacca gagcuaguuu uaaggcuaaa 300aaaccaacca auuuucuaag cauuguagaa guuuguguuu agggcaagau uuuuggacug 360ggguucgauu ccccacagcu ccacca 386<210> 45<211> 312<212> DNA<213> Klebsiella aerogenes (NCTC 9528)<400> 45gggattcgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa 60aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc taagagccct 120ctctccctag cttccgctcc taagacgggg aataaagaga ggtcaaaccc aaaagagatc 180gcgtggaagc cctgcctggg gttgaagcgt taaaactaat caggctagtt tgtcagtggc 240gtgtccgtcc gcagctggcc agcgaatgta aagactggac taagcatgta gtgccgagga 300tgtaggaatt tc 312<210> 46<211> 312<212> RNA<213> Klebsiella aerogenes (NCTC 9528)<400> 46gggauucgcg aaacccaagg ugcaugccga ggggcgguug gccucguaaa aagccgcaaa 60aaaauagucg caaacgacga aaacuacgcu uuagcagcuu aauaaccugc uaagagcccu 120cucucccuag cuuccgcucc uaagacgggg aauaaagaga ggucaaaccc aaaagagauc 180gcguggaagc ccugccuggg guugaagcgu uaaaacuaau caggcuaguu ugucaguggc 240guguccgucc gcagcuggcc agcgaaugua aagacuggac uaagcaugua gugccgagga 300uguaggaauu uc 312<210> 47<211> 316<212> DNA<213> Lactobacillus lactis (NCTC 662)<400> 47aagcacagtt cgagcttgaa ttgcgtttcg taggttacgt ctacgttaaa acgttacagt 60taaatataac tgctaaaaac gaaaacaact cttacgcttt agctgcctaa aaacagttag 120cgtagatcct ctcggcatcg cccatgtgct cgagtaaggg tctcaaattt agtgggatac 180gttaaacttt tccgtctgta aagtttaaaa gagatcatca gactagcgat acagaatgcc 240tgtcactcgg caagctgtaa agcgaaacct caaatgagtt gactatgaac gtagattttt 300aagtgtcgat gtgttt 316<210> 48<211> 316<212> RNA<213> Lactobacillus lactis (NCTC 662)<400> 48aagcacaguu cgagcuugaa uugcguuucg uagguuacgu cuacguuaaa acguuacagu 60uaaauauaac ugcuaaaaac gaaaacaacu cuuacgcuuu agcugccuaa aaacaguuag 120cguagauccu cucggcaucg cccaugugcu cgaguaaggg ucucaaauuu agugggauac 180guuaaacuuu uccgucugua aaguuuaaaa gagaucauca gacuagcgau acagaaugcc 240ugucacucgg caagcuguaa agcgaaaccu caaaugaguu gacuaugaac guagauuuuu 300aagugucgau guguuu 316<210> 49<211> 362<212> DNA<213> Legionella pneumophila<400> 49gtgggttgca aaaccggaag tgcatgccga gaaggagatc tctcgtaaat aagactcaat 60taaatataaa tgcaaacgat gaaaactttg ctggtgggga agctatcgct gcctaataag 120cactttagtt aaaccatcac tgtgtactgg ccaataaacc cagtatcccg ttcgaccgag 180cccgcttatc ggtatcgaat caacggtcat aagagataag ctagcgtcct aatctatccc 240gggttatggc gcgaaactca gggaatcgct gtgtatcatc ctgcccgtcg gaggagccac 300agttaaattc aaaagacaag gctatgcatg tagagctaaa ggcagaggac ttgcggacgc 360gg362<210> 50<211> 362<212> RNA<213> Legionella pneumophila<400> 50guggguugca aaaccggaag ugcaugccga gaaggagauc ucucguaaau aagacucaau 60uaaauauaaa ugcaaacgau gaaaacuuug cuggugggga agcuaucgcu gccuaauaag 120cacuuuaguu aaaccaucac uguguacugg ccaauaaacc caguaucccg uucgaccgag 180cccgcuuauc gguaucgaau caacggucau aagagauaag cuagcguccu aaucuauccc 240ggguuauggc gcgaaacuca gggaaucgcu guguaucauc cugcccgucg gaggagccac 300aguuaaauuc aaaagacaag gcuaugcaug uagagcuaaa ggcagaggac uugcggacgc 360gg362<210> 51<211> 322<212> DNA<213> Listeria grayi<400> 51acagggatag gtcgagcttg agttgcgagc cggggggatc ggcccgtcat caacgtcaaa 60gccaataata actggcaaac aaaacaacaa tttagctttc gctgcctaat agcagtctga 120atagctgatc ctccgtgcat cacccatgtg ctacggtaag ggtctcactt ttaagtgggt 180tacgctggct tatctccgtc tggggcaaac gagaagagca taatcagact agctagatag 240agccctgacg ccgggcagac atctatgcga aatccaaata cggcaactac gctcgtagat 300gctcaagtgc cgatatttct gg 322<210> 52<211> 322<212> RNA<213> Listeria grayi<400> 52acagggauag gucgagcuug aguugcgagc cggggggauc ggcccgucau caacgucaaa 60gccaauaaua acuggcaaac aaaacaacaa uuuagcuuuc gcugccuaau agcagucuga 120auagcugauc cuccgugcau cacccaugug cuacgguaag ggucucacuu uuaagugggu 180uacgcuggcu uaucuccguc uggggcaaac gagaagagca uaaucagacu agcuagauag 240agcccugacg ccgggcagac aucuaugcga aauccaaaua cggcaacuac gcucguagau 300gcucaagugc cgauauuucu gg 322<210> 53<211> 322<212> DNA<213> Listeria innocua<400> 53acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactagtt aatctccgtc tgaggttaaa tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300attcaagtgc cgatatttct gg 322<210> 54<211> 322<212> RNA<213> Listeria innocua<400> 54acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuaguu aaucuccguc ugagguuaaa uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auucaagugc cgauauuucu gg 322<210> 55<211> 322<212> DNA<213> Listeria monocytogenes (NCTC 7973)<400> 55acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactagtt aatctccgtc tggggttaaa tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggccgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg 322<210> 56<211> 322<212> RNA<213> Listeria monocytogenes (NCTC 7973)<400> 56acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuaguu aaucuccguc ugggguuaaa uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggccgau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auuuaagugc cgauauuucu gg 322<210> 57<211> 247<212> DNA<213> Listeria monocytogenes (NCTC 11994)<400> 57caaagccaat aataactggc aaagaaaaac aaaacctagc tttcgctgcc taataagcag 60tagcatagct gatcctccgt gcatcgccca tgtgctacgg taagggtctc actctaagtg 120ggctacacta gttaatctcc gtctggggtt aaatagaaga gcttaatcag actagctgaa 180tggaagcctg ttaccgggcc gatgtttatg cgaaatgcta atacggtgac tacgctcgta 240gatattt 247<210> 58<211> 247<212> RNA<213> Listeria monocytogenes (NCTC 11994)<400> 58caaagccaau aauaacuggc aaagaaaaac aaaaccuagc uuucgcugcc uaauaagcag 60uagcauagcu gauccuccgu gcaucgccca ugugcuacgg uaagggucuc acucuaagug 120ggcuacacua guuaaucucc gucugggguu aaauagaaga gcuuaaucag acuagcugaa 180uggaagccug uuaccgggcc gauguuuaug cgaaaugcua auacggugac uacgcucgua 240gauauuu 247<210> 59<211> 322<212> DNA<213> Listeria murrayi<400> 59acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactagtt aatctccgtc tgaggttaaa tagaagagct taatgagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300attcaagtgc cgatatttct gg 322<210> 60<211> 322<212> RNA<213> Listeria murrayi<400> 60acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuaguu aaucuccguc ugagguuaaa uagaagagcu uaaugagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auucaagugc cgauauuucu gg 322<210> 61<211> 322<212> DNA<213> Listeria welshimeri<400> 61acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ctaagtgggc 180tacactggct aatctccgtc tgaggttagt tggaagagct taatcagact agctgaatgg 240aagcctgtta ccgggccgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg 322<210> 62<211> 322<212> RNA<213> Listeria welshimeri<400> 62acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu cuaagugggc 180uacacuggcu aaucuccguc ugagguuagu uggaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggccgau guuuaugcga aaugcuaaua cggugacuac gcucguagau 300auuuaagugc cgauauuucu gg 322<210> 63<211> 322<212> DNA<213> Marinobacter hydrocarbonoclasticus<400> 63gccggtgacg aacccttggg tgcatgccga gatggcagcg aatctcgtaa atccaaagct 60gcaacgtaat agtcgcaaac gacgaaaact acgcactggc ggcgtaagcc gttccagtcg 120tcctggctga ggcgcctata actcagtagc aacatcccag gacgtcatcg cttataggct 180gctccgttca ccagagctca ctggtgttcg gctaagatta aagagctcgc ctcttgcacc 240ctgaccttcg ggtcgcttga ggttaaatca atagaaggac actaagcatg tagacctcaa 300ggcctagtgc tggcggacgc gg 322<210> 64<211> 322<212> RNA<213> Marinobacter hydrocarbonoclasticus<400> 64gccggugacg aacccuuggg ugcaugccga gauggcagcg aaucucguaa auccaaagcu 60gcaacguaau agucgcaaac gacgaaaacu acgcacuggc ggcguaagcc guuccagucg 120uccuggcuga ggcgccuaua acucaguagc aacaucccag gacgucaucg cuuauaggcu 180gcuccguuca ccagagcuca cugguguucg gcuaagauua aagagcucgc cucuugcacc 240cugaccuucg ggucgcuuga gguuaaauca auagaaggac acuaagcaug uagaccucaa 300ggccuagugc uggcggacgc gg 322<210> 65<211> 338<212> DNA<213> Mycobacterium avium<400> 65ttcgcgcatc gaatcaaggg aagcgtgccg gtgcaggcaa ctgaccaccg taagcgtcgt 60tgcaaataga taagcgccga ttcacatcag cgcgacttac ctctcgctgc ctaagcgaca 120gctagtccgt cagcccggga acgccctcga cccggagcct ggcgtcagct agagggatcc 180accgatgagt tcggtcgcgg gactcatcgg gacaccaaca gcgactggga tcgtcatcct 240ggcttgttcg cgtgaccagg agatccgagt agaggcatag cgaactgcgc acggagaagc 300cttgagggaa tgccgtagaa cccgggttcg attcccaa 338<210> 66<211> 338<212> RNA<213> Mycobacterium avium<400> 66uucgcgcauc gaaucaaggg aagcgugccg gugcaggcaa cugaccaccg uaagcgucgu 60ugcaaauaga uaagcgccga uucacaucag cgcgacuuac cucucgcugc cuaagcgaca 120gcuaguccgu cagcccggga acgcccucga cccggagccu ggcgucagcu agagggaucc 180accgaugagu ucggucgcgg gacucaucgg gacaccaaca gcgacuggga ucgucauccu 240ggcuuguucg cgugaccagg agauccgagu agaggcauag cgaacugcgc acggagaagc 300cuugagggaa ugccguagaa cccggguucg auucccaa 338<210> 67<211> 318<212> DNA<213> Mycobacterium bovis<400> 67ttcgcgcatc gaatcaaggg aagcgtgccg gtgcaggcaa gagaccaccg taagcgtcgt 60tgcgaccaaa taagcgccga ttcacatcag cgcgactacg tctcgctgcc taagcgacgg 120ctagtctgtc agaccgggaa cgccctcggc ccggaccctg gcatcagcta gagggatcca 180ccgatgagtc cggtcgcggg actcctcggg acaaccacag cgactgggat cgtcatctcg 240gctagttcgc gtgaccggga gatccgagca gaggcatagc gaactgcgca cggagaagcc 300ttgagggaat gccgtagg 318<210> 68<211> 318<212> RNA<213> Mycobacterium bovis<400> 68uucgcgcauc gaaucaaggg aagcgugccg gugcaggcaa gagaccaccg uaagcgucgu 60ugcgaccaaa uaagcgccga uucacaucag cgcgacuacg ucucgcugcc uaagcgacgg 120cuagucuguc agaccgggaa cgcccucggc ccggacccug gcaucagcua gagggaucca 180ccgaugaguc cggucgcggg acuccucggg acaaccacag cgacugggau cgucaucucg 240gcuaguucgc gugaccggga gauccgagca gaggcauagc gaacugcgca cggagaagcc 300uugagggaau gccguagg 318<210> 69<211> 369<212> DNA<213> Mycobacterium leprae<400> 69ggggctgaaa ggtttcgact tcgcgcatcg aatcaaggga agcgtgccgg tgcaggcaag 60agaccaccgt aagcgtcgtt gcagcaatat aagcgccgat tcatatcagc gcgactatgc 120tctcgctgcc taagcgatgg ctagtctgtc agaccgggaa cgccctcgtc ccggagcctg 180gcatcagcta gagggatcta ccgatgggtt cggtcgcggg actcgtcggg acaccaaccg 240cgactgggat cgtcatcctg gctagttcgc gtgatcagga gatccgagta gaggcatagc 300gaactacgca cggagaagcc ttgagggaaa tgccgtagga cccgggttcg attcccggca 360gctccacca 369<210> 70<211> 369<212> RNA<213> Mycobacterium leprae<400> 70ggggcugaaa gguuucgacu ucgcgcaucg aaucaaggga agcgugccgg ugcaggcaag 60agaccaccgu aagcgucguu gcagcaauau aagcgccgau ucauaucagc gcgacuaugc 120ucucgcugcc uaagcgaugg cuagucuguc agaccgggaa cgcccucguc ccggagccug 180gcaucagcua gagggaucua ccgauggguu cggucgcggg acucgucggg acaccaaccg 240cgacugggau cgucauccug gcuaguucgc gugaucagga gauccgagua gaggcauagc 300gaacuacgca cggagaagcc uugagggaaa ugccguagga cccggguucg auucccggca 360gcuccacca 369<210> 71<211> 338<212> DNA<213> Mycobacterium paratuberculosis<400> 71ttcgcgcatc gaatcaaggg aagcgtgccg gtgcaggcaa ctgaccaccg taagcgtcgt 60tgcaaataga taagcgccga ttcacatcag cgcgacttac ctctcgctgc ctaagcgaca 120gctagtccgt cagcccggga acgccctcga cccggagcct ggcgtcagct agagggatcc 180accgatgagt tcggtcgcgg gactcatcgg gacaccaaca gcgactggga tcgtcatcct 240ggcttgttcg cgtgaccagg agatccgagt agaggcatag cgaactgcgc acggagaagc 300cttgagggaa tgccgtagaa cccgggttcg attcccaa 338<210> 72<211> 338<212> RNA<213> Mycobacterium paratuberculosis<400> 72uucgcgcauc gaaucaaggg aagcgugccg gugcaggcaa cugaccaccg uaagcgucgu 60ugcaaauaga uaagcgccga uucacaucag cgcgacuuac cucucgcugc cuaagcgaca 120gcuaguccgu cagcccggga acgcccucga cccggagccu ggcgucagcu agagggaucc 180accgaugagu ucggucgcgg gacucaucgg gacaccaaca gcgacuggga ucgucauccu 240ggcuuguucg cgugaccagg agauccgagu agaggcauag cgaacugcgc acggagaagc 300cuugagggaa ugccguagaa cccggguucg auucccaa 338<210> 73<211> 368<212> DNA<213> Mycobacterium tuberculosis<400> 73ggggctgaac ggtttcgact tcgcgcatcg aatcaaggga agcgtgccgg tgcaggcaag 60agaccaccgt aagcgtcgtt gcgaccaaat aagcgccgat tcacatcagc gcgactacgc 120tctcgctgcc taagcgacgg ctagtctgtc agaccgggaa cgccctcggc ccggaccctg 180gcatcagcta gagggatcca ccgatgagtc cggtcgcggg actcctcggg acaaccacag 240cgactgggat cgtcatctcg gctagttcgc gtgaccggga gatccgagca gaggcatagc 300gaactgcgca cggagaagcc ttgagggaat gccgtaggac ccgggttcga ttcccggcag 360ctccacca 368<210> 74<211> 368<212> RNA<213> Mycobacterium tuberculosis<400> 74ggggcugaac gguuucgacu ucgcgcaucg aaucaaggga agcgugccgg ugcaggcaag 60agaccaccgu aagcgucguu gcgaccaaau aagcgccgau ucacaucagc gcgacuacgc 120ucucgcugcc uaagcgacgg cuagucuguc agaccgggaa cgcccucggc ccggacccug 180gcaucagcua gagggaucca ccgaugaguc cggucgcggg acuccucggg acaaccacag 240cgacugggau cgucaucucg gcuaguucgc gugaccggga gauccgagca gaggcauagc 300gaacugcgca cggagaagcc uugagggaau gccguaggac ccggguucga uucccggcag 360cuccacca 368<210> 75<211> 411<212> DNA<213> Mycoplasma capricolum<400> 75ggggatgtca tggatttgac aggatatctt tagtacatat aagcagtagt gttgtagact 60ataaatacta ctaggtttaa aaaaacgcaa ataaaaacga agaaactttt gaaatgccag 120catttatgat gaataatgca tcagctggag caaactttat gtttgcttaa taactactag 180tttagttata gtatttcacg aattatagat attttaagct ttatttataa ccgtattacc 240caagcttaat agaatatatg attgcaataa atatatttga aatctaattg caaatgatat 300ttaaccttta gttaatttta gttaaatatt ttaattagaa aattaactaa actgtagaaa 360gtatgtatta atatatcttg gacgcgagtt cgattctcgc catctccacc a 411<210> 76<211> 411<212> RNA<213> Mycoplasma capricolum<400> 76ggggauguca uggauuugac aggauaucuu uaguacauau aagcaguagu guuguagacu 60auaaauacua cuagguuuaa aaaaacgcaa auaaaaacga agaaacuuuu gaaaugccag 120cauuuaugau gaauaaugca ucagcuggag caaacuuuau guuugcuuaa uaacuacuag 180uuuaguuaua guauuucacg aauuauagau auuuuaagcu uuauuuauaa ccguauuacc 240caagcuuaau agaauauaug auugcaauaa auauauuuga aaucuaauug caaaugauau 300uuaaccuuua guuaauuuua guuaaauauu uuaauuagaa aauuaacuaa acuguagaaa 360guauguauua auauaucuug gacgcgaguu cgauucucgc caucuccacc a 411<210> 77<211> 388<212> DNA<213> Mycoplasma genitalium (ATTC 33530，#1)<400> 77ggggatgttt tgggtttgac ataatgctga tagacaaaca gtagcattgg ggtatgcccc 60ttacagcgct aggttcaata accgacaaag aaaataacga agtgttggta gaaccaaatt 120tgatcattaa ccaacaagca agtgttaact ttgcttttgc ataagtagat actaaagcta 180cagctggtga atagtcatag tttgctagct gtcatagttt atgactcgag gttaaatcgt 240tcaatttaac ctttaaaaat agaacttgtt gtttccatga ttgttttgtg atcaattgga 300aacaagacaa aaatccacaa aactaaaatg tagaagctgt ttgttgtgtc ctttatggaa 360acgggttcga ttcccgtcat ctccacca388<210> 78<211> 388<212> RNA<213> Mycoplasma genitalium (ATTC 33530，#1)<400> 78ggggauguuu uggguuugac auaaugcuga uagacaaaca guagcauugg gguaugcccc 60uuacagcgcu agguucaaua accgacaaag aaaauaacga aguguuggua gaaccaaauu 120ugaucauuaa ccaacaagca aguguuaacu uugcuuuugc auaaguagau acuaaagcua 180cagcugguga auagucauag uuugcuagcu gucauaguuu augacucgag guuaaaucgu 240ucaauuuaac cuuuaaaaau agaacuuguu guuuccauga uuguuuugug aucaauugga 300aacaagacaa aaauccacaa aacuaaaaug uagaagcugu uuguuguguc cuuuauggaa 360acggguucga uucccgucau cuccacca388<210> 79<211> 243<212> RNA<213> Mycoplasma genitalium (ATTC 33530，#2)<400> 79acaaagcgaa gacaaacaga gcaggggagc cccacagcgc aggcaaaacc gacaaagaaa 60aaacgaaggg gagaccaaag acaaaccaac aagcaaggaa cgcgcaaaga gaacaaagca 120cagcgggaaa gcaaggcagc gcaagagacc gaggaaacgc aaaaccaaaa aagaacggcc 180agagggacaa ggaaacaaga caaaaaccac aaaacaaaag agaagcgggg ccaggaaacg 240ggc 243<210> 80<211> 350<212> RNA<213> Mycoplasma genitalium (ATTC 33530，#2)<400> 80acauaaugcu gauagacaaa caguagcauu gggguaugcc ccuuacagcg cuagguucaa 60uaaccgacaa agaaaauaac gaaguguugg uagauccaaa uuugaucauu aaccaacaag 120caaguguuaa cuuugcuuuu gcauaaguag auacuaaagc uacagcuggu gaauagucau 180aguuugcuag cugucauagu uuaugacucg agguuaaauc guucaauuua accuuuaaaa 240auagaacuug uuguuuccau gauuguuuug ugaucaauug gaaacaagac aaaaauccac 300aaaacuaaaa uguagaagcu guuuguugug uccuuuaugg aaacggguuc350<210> 81<211> 387<212> DNA<213> Mycoplasma pneumophila<400> 81ggggatgtag aggttttgac ataatgttga aaggaaaaca gttgcagtgg ggtatgcccc 60ttacagctct aggtataata accgacaaaa ataacgacga agttttggta gatccaatgt 120tgatcgctaa ccaacaagca agtatcaact acgctttcgc ttagaacata ctaaagctac 180acgaattgaa tcgccatagt ttggttcgtg tcacagttta tggctcgggg ttaactggtt 240caacttaatc cttaaattat gaacttatcg tttacttgtt tgtcttatga tctaaagtaa 300gcgagacatt aaaacataag actaaactgt agaagctgtt ttaccaatcc tttatggaaa 360cgggttcgat tcccgtcatc tccacca 387<210> 82<211> 387<212> RNA<213> Mycoplasma pneumophila<400> 82ggggauguag agguuuugac auaauguuga aaggaaaaca guugcagugg gguaugcccc 60uuacagcucu agguauaaua accgacaaaa auaacgacga aguuuuggua gauccaaugu 120ugaucgcuaa ccaacaagca aguaucaacu acgcuuucgc uuagaacaua cuaaagcuac 180acgaauugaa ucgccauagu uugguucgug ucacaguuua uggcucgggg uuaacugguu 240caacuuaauc cuuaaauuau gaacuuaucg uuuacuuguu ugucuuauga ucuaaaguaa 300gcgagacauu aaaacauaag acuaaacugu agaagcuguu uuaccaaucc uuuauggaaa 360cggguucgau ucccgucauc uccacca 387<210> 83<211> 318<212> DNA<213> Neisseria gonorrhoeae (ATCC 19424)<400> 83gggggttgcg aagcagatgc gggcataccg gggtctcaga ttcccgtaaa acactgaatt 60caaatagtcg caaacgacga aacttacgct ttagccgctt aaggctagcc gttgcagcag 120tcggtcaatg ggctgtgtgg cgaaagccac cgcaacgtca tcttacattg actggtttcc 180agccgggtta cttggcagga aataagactt aaggtaactg gtttccaaaa ggcctgttgg 240tcggcatgat ggaaataaga ttttcaaata gacacaacta agtatgtaga acgctttgta 300gaggactttc ggacgggg 318<210> 84<211> 318<212> RNA<213> Neisseria gonorrhoeae (ATCC 19424)<400> 84ggggguugcg aagcagaugc gggcauaccg gggucucaga uucccguaaa acacugaauu 60caaauagucg caaacgacga aacuuacgcu uuagccgcuu aaggcuagcc guugcagcag 120ucggucaaug ggcugugugg cgaaagccac cgcaacguca ucuuacauug acugguuucc 180agccggguua cuuggcagga aauaagacuu aagguaacug guuuccaaaa ggccuguugg 240ucggcaugau ggaaauaaga uuuucaaaua gacacaacua aguauguaga acgcuuugua 300gaggacuuuc ggacgggg 318<210> 85<211> 363<212> DNA<213> Neisseria gonorrhoeae (FA 1090)<400> 85gggggcgacc ttggtttcga cgggggttgc gaagcagatg cgggcatacc ggggtctcag 60attcccgtaa aacactgaat tcaaatagtc gcaaacgacg aaacttacgc tttagccgct 120taaggctagc cgttgcagca gtcggtcaat gggctgtgtg gtgaaagcca ccgcaacgtc 180atcttacatt gactggtttc cagccgggtt acttggcagg aaataagact taaggtaact 240ggtttccaaa aggcctgttg gtcggcatga tggaaataag attttcaaat agacacaact 300aagtatgtag aacgctttgt agaggacttt cggacggggg ttcgattccc cccgcctcca 360cca 363<210> 86<211> 363<212> RNA<213> Neisseria gonorrhoeae (FA 1090)<400> 86gggggcgacc uugguuucga cggggguugc gaagcagaug cgggcauacc ggggucucag 60auucccguaa aacacugaau ucaaauaguc gcaaacgacg aaacuuacgc uuuagccgcu 120uaaggcuagc cguugcagca gucggucaau gggcugugug gugaaagcca ccgcaacguc 180aucuuacauu gacugguuuc cagccggguu acuuggcagg aaauaagacu uaagguaacu 240gguuuccaaa aggccuguug gucggcauga uggaaauaag auuuucaaau agacacaacu 300aaguauguag aacgcuuugu agaggacuuu cggacggggg uucgauuccc cccgccucca 360cca 363<210> 87<211> 363<212> DNA<213> Neisseria meningitidis<400> 87gggggcgacc ttggtttcga cgggggttgc gaagcagatg cgggcataccggggtctcag 60attcccgtaa aacactgaat tcaaatagtc gcaaacgacg aaacttacgc tttagccgct 120taaggctagc cgttgcagca gtcggtcaat gggctgtgtg gcgaaagcca ccgcaacgtc 180atcttacatt gactggtttc ctgccgggtt atttggcagg aaatgagatt taaggtaact 240ggtttccaaa aggcctgttg gtcggcatga tggaaataag attttcaaat agacacaact 300aagtatgtag aacgctttgt agaggacttt cggacggggg ttcgattccc cccgcctcca 360cca 363<210> 88<211> 363<212> RNA<213> Neisseria meningitidis<400> 88gggggcgacc uugguuucga cggggguugc gaagcagaug cgggcauacc ggggucucag 60auucccguaa aacacugaau ucaaauaguc gcaaacgacg aaacuuacgc uuuagccgcu 120uaaggcuagc cguugcagca gucggucaau gggcugugug gcgaaagcca ccgcaacguc 180aucuuacauu gacugguuuc cugccggguu auuuggcagg aaaugagauu uaagguaacu 240gguuuccaaa aggccuguug gucggcauga uggaaauaag auuuucaaau agacacaacu 300aaguauguag aacgcuuugu agaggacuuu cggacggggg uucgauuccc cccgccucca 360cca 363<210> 89<211> 385<212> DNA<213> Nostoc muscorum PCC7120<400> 89gggtccgtcg gtttcgacag gttggcgaac gctactctgt gattcaggtc gagagtgagt 60ctcctctgca aatcaaggct caaaacaaaa gtaaatgcga ataacatcgt taaatttgct 120cgtaaggacg ctctagtagc tgcctaaata gcctctttca ggttcgagcg tcttcggttt 180gactccgtta aggactgaag accaaccccc aacggatgct ctagcaatgt tctctggttg 240gcttgctagc taagatttaa tcagagcatc ctacgttcgg gataatgaac gattcccgcc 300ttgagggtca gaaaggctaa acctgtgaat gagcgggggg tcaataccca atttggacag 360cagttcgact ctgctcgatc cacca 385<210> 90<211> 385<212> RNA<213> Nostoc muscorum PCC7120<400> 90ggguccgucg guuucgacag guuggcgaac gcuacucugu gauucagguc gagagugagu 60cuccucugca aaucaaggcu caaaacaaaa guaaaugcga auaacaucgu uaaauuugcu 120cguaaggacg cucuaguagc ugccuaaaua gccucuuuca gguucgagcg ucuucgguuu 180gacuccguua aggacugaag accaaccccc aacggaugcu cuagcaaugu ucucugguug 240gcuugcuagc uaagauuuaa ucagagcauc cuacguucgg gauaaugaac gauucccgcc 300uugaggguca gaaaggcuaa accugugaau gagcgggggg ucaauaccca auuuggacag 360caguucgacu cugcucgauc cacca 385<210> 91<211> 371<212> DNA<213> Odontella sinensis(硅藻屬)葉綠體<400> 91ggggctgact tggtttcgac atttaaaaat tgttacagta tgatgcaggt cgaagtttct 60aatcttcgta aaaaaagaga aatttataat aaatgctaat aatttaattt cttctgtgtt 120taaaagttta tcaactaagc aaaatagttt aaatttaagt tttgctgttt aagttttatg 180cacatttaat gatctagtaa ataactttgt tcgctataat ttatatttat aactagactt 240ttgtcttttt tatagtttag aataacttta tcatttcaaa cctcgttcca tctagttgaa 300ctaaacctgt gaacgaatac tataataaaa tttttagatg gacgtgggtt cgactcccat 360cagctccacc a 371<210> 92<211> 371<212> RNA<213> Odontella sinensis(硅藻屬)葉綠體<400> 92ggggcugacu ugguuucgac auuuaaaaau uguuacagua ugaugcaggu cgaaguuucu 60aaucuucgua aaaaaagaga aauuuauaau aaaugcuaau aauuuaauuu cuucuguguu 120uaaaaguuua ucaacuaagc aaaauaguuu aaauuuaagu uuugcuguuu aaguuuuaug 180cacauuuaau gaucuaguaa auaacuuugu ucgcuauaau uuauauuuau aacuagacuu 240uugucuuuuu uauaguuuag aauaacuuua ucauuucaaa ccucguucca ucuaguugaa 300cuaaaccugu gaacgaauac uauaauaaaa uuuuuagaug gacguggguu cgacucccau 360cagcuccacc a 371<210> 93<211> 323<212> DNA<213>Porphyra purpureum(紅藻)葉綠體<400> 93ggggctgcaa ggtttctaca ttgtgaaaaa acaaatatat gaaagtaaaa cgagctcatt 60attagagctt ttagttaaat aaatgcagaa aataatatta ttgctttttc tcgaaaatta 120gctgttgcat aaatagtctc aatttttgta attcgaagtg atagactctt atacactacg 180aatattctgt tagagttgct cttaataaaa gaaaagtaaa aaaatacaaa ttcttatgtt 240ttttacctga attgattcaa tttaaggtta gtattttttg atttttacaa tggacgtggg 300ttcaagtccc accagctcca cca 323<210> 94<211> 323<212> RNA<213>Porphyra purpureum(紅藻)葉綠體<400> 94ggggcugcaa gguuucuaca uugugaaaaa acaaauauau gaaaguaaaa cgagcucauu 60auuagagcuu uuaguuaaau aaaugcagaa aauaauauua uugcuuuuuc ucgaaaauua 120gcuguugcau aaauagucuc aauuuuugua auucgaagug auagacucuu auacacuacg 180aauauucugu uagaguugcu cuuaauaaaa gaaaaguaaa aaaauacaaa uucuuauguu 240uuuuaccuga auugauucaa uuuaagguua guauuuuuug auuuuuacaa uggacguggg 300uucaaguccc accagcucca cca 323<210> 95<211> 407<212> DNA<213> Porphyromonas gingivalis<400> 95ggggctgacc ggctttgaca gcgtgatgaa gcggtatgta agcatgtagt gcgtgggtgg 60cttgcactat aatctcagac atcaaaagtt taattggcga aaataactac gctctcgctg 120cgtaatcgaa gaatagtaga ttagacgctt catcgccgcc aaagtggcag cgacgagaca 180tcgcccgagc agctttttcc cgaagtagct cgatggtgcg gtgctgacaa atcgggaacc 240gctacaggat gcttcctgcc tgtggtcaga tcgaacggaa gataaggatc gtgcattggg 300tcgtttcagc ctccgctcgc tcacgaaaat tccaactgaa actaaacatg tagaaagcat 360attgattcca tgtttggacg agggttcaat tccctccagc tccacca 407<210> 96<211> 407<212> RNA<213> Porphyromonas gingivalis<400> 96ggggcugacc ggcuuugaca gcgugaugaa gcgguaugua agcauguagu gcgugggugg 60cuugcacuau aaucucagac aucaaaaguu uaauuggcga aaauaacuac gcucucgcug 120cguaaucgaa gaauaguaga uuagacgcuu caucgccgcc aaaguggcag cgacgagaca 180ucgcccgagc agcuuuuucc cgaaguagcu cgauggugcg gugcugacaa aucgggaacc 240gcuacaggau gcuuccugcc uguggucaga ucgaacggaa gauaaggauc gugcauuggg 300ucguuucagc cuccgcucgc ucacgaaaau uccaacugaa acuaaacaug uagaaagcau 360auugauucca uguuuggacg aggguucaau ucccuccagc uccacca 407<210> 97<211> 310<212> DNA<213> Proteus rettgeri (NCTC 10975)<400> 97gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa 60aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct 120ctctccctag cctccgctct tggacgggga tcaagagagg tcaaacccaa aagagatcgc 180gtggatgcct tgcctggggt tgaagcgtta aacttaatca ggatagtttg ttggtggcgt 240gtctgtccgc agctggcaaa tgaattcaaa gactagacta agcatgtagt accgaggatg 300tagaaatttc310<210> 98<211> 310<212> RNA<213> Proteus rettgeri (NCTC 10975)<400> 98gggauuugcg aaacccaagg ugcaugccga ggggcgguug gccucguaaa aagccgcaaa 60aaaauagucg caaacgacga aaacuacgcu uuagcagcuu aauaaccugc uuagagcccu 120cucucccuag ccuccgcucu uggacgggga ucaagagagg ucaaacccaa aagagaucgc 180guggaugccu ugccuggggu ugaagcguua aacuuaauca ggauaguuug uugguggcgu 240gucuguccgc agcuggcaaa ugaauucaaa gacuagacua agcauguagu accgaggaug 300uagaaauuuc310<210> 99<211> 241<212> DNA<213> Pseudoalteromonas haloplanktis<400> 99gggagcgaaa cccaagggca gccgaggggc ggggcccgaa aaagccgcaa aaaaaagcgc 60aaacgacgaa aacacgcagc agcaaaaccg cagagccccc cccagccccg ccggacgggg 120acaagagagg caaacccaaa agagacgcgg gagccgccgg gggaagcgaa acaacaggaa 180ggggggcggc gccgcagcgg caaagaacaa agacagacaa gcagagaccg aggagagaaa 240c 241<210> 100<211> 313<212> RNA<213> Pseudoalteromonas haloplanktis<400> 100ggaauucaag aagcccgagg ugcaugucga ggugcgguuu gccucguaaa aaagccgcaa 60uuuaaaguaa ucgcaaacga cgauaacuac ucucuagcag cuuaggcugg cuagcgcucc 120uuccauguau ucuuguggac uggauuuugg agugucaccc uaacaccuga ucgcgacgga 180aacccuggcc gggguugaag cguuaaaacu aagcggccuc gccuuuaucu accguguuug 240uccgggauuu aaagguuaau uaaaugacaa uacuaaacau guaguaccga cggucgaggc 300uuuucggacg ggg313<210> 101<211> 353<212> DNA<213> Pseudomonas aeruginosa<400> 101ggggccgatt aggattcgac gccggtaaca aaagttgagg ggcatgccga gttggtagca 60gaactcgtaa attcgctgct gcaaacttat agttgccaac gacgacaact acgctctagc 120tgcttaatgc ggctagcagt cgctagggga tgcctgtaaa cccgaaacga ctgtcagata 180gaacaggatc gccgccaagt tcgctgtaga cgtaacggct aaaactcata cagctcgctc 240caagcaccct gccactcggg cggcgcggag ttaactcagt agagctggct aagcatgtaa 300aaccgatagc ggaaagctgg cggacggggg ttcaaatccc cccggttcca cca353<210> 102<211> 353<212> RNA<213> Pseudomonas aeruginosa<400> 102ggggccgauu aggauucgac gccgguaaca aaaguugagg ggcaugccga guugguagca 60gaacucguaa auucgcugcu gcaaacuuau aguugccaac gacgacaacu acgcucuagc 120ugcuuaaugc ggcuagcagu cgcuagggga ugccuguaaa cccgaaacga cugucagaua 180gaacaggauc gccgccaagu ucgcuguaga cguaacggcu aaaacucaua cagcucgcuc 240caagcacccu gccacucggg cggcgcggag uuaacucagu agagcuggcu aagcauguaa 300aaccgauagc ggaaagcugg cggacggggg uucaaauccc cccgguucca cca353<210> 103<211> 363<212> DNA<213> Salmonella typhimurium<400> 103ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt 60ggcctcgtaa aaagccgcaa aaaaatagtc gcaaacgacg aaacctacgc tttagcagct 120taataacctg cttagagccc tctctcccta gcctccgctc ttaggacggg gatcaagaga 180ggtcaaaccc aaaagagatc gcgcggatgc cctgcctggg gttgaagcgt taaaacgaat 240caggctagtc tggtagtggc gtgtccgtcc gcaggtgcca ggcgaatgta aagactgact 300aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca 360cca 363<210> 104<211> 363<212> RNA<213> Salmonella typhimurium<400> 104ggggcugauu cuggauucga cgggauuugc gaaacccaag gugcaugccg aggggcgguu 60ggccucguaa aaagccgcaa aaaaauaguc gcaaacgacg aaaccuacgc uuuagcagcu 120uaauaaccug cuuagagccc ucucucccua gccuccgcuc uuaggacggg gaucaagaga 180ggucaaaccc aaaagagauc gcgcggaugc ccugccuggg guugaagcgu uaaaacgaau 240caggcuaguc ugguaguggc guguccgucc gcaggugcca ggcgaaugua aagacugacu 300aagcauguag uaccgaggau guaggaauuu cggacgcggg uucaacuccc gccagcucca 360cca 363<210> 105<211> 355<212> DNA<213> Shewanella putrefaciens<400> 105gggggcgatt ctggattcga caggattcac gaaaccctgg gagcatgccg aggggcggtt 60ggcctcgtaa aaagccgcaa agttatagtt gcaaacgacg ataactacgc tctagccgct 120taatgccgct agccatctac cacacgcttt gcacatgggc agtggatttg atggtcatct 180cacatcgtgc tagcgaggga accctgtctg ggggtgaacc gcgaaacagt accggactca 240ccgtgtggga tcctgtcttt cggagttcaa acggttaaac aatagaaaga ctaagcatgt 300agcgccttgg atgtaggttt tctggacgcg ggttcaagtc ccgccgcctc cacca 355<210> 106<211> 355<212> RNA<213> Shewanella putrefaciens<400> 106gggggcgauu cuggauucga caggauucac gaaacccugg gagcaugccg aggggcgguu 60ggccucguaa aaagccgcaa aguuauaguu gcaaacgacg auaacuacgc ucuagccgcu 120uaaugccgcu agccaucuac cacacgcuuu gcacaugggc aguggauuug auggucaucu 180cacaucgugc uagcgaggga acccugucug ggggugaacc gcgaaacagu accggacuca 240ccguguggga uccugucuuu cggaguucaa acgguuaaac aauagaaaga cuaagcaugu 300agcgccuugg auguagguuu ucuggacgcg gguucaaguc ccgccgccuc cacca 355<210> 107<211> 362<212> DNA<213> Staphylococcus aureus<400> 107ggggacgttc atggattcga caggggtccc ccgagctcat taagcgtgtc ggagggttgt 60cttcgtcatc aacacacaca gtttataata actggcaaat caaacaataa tttcgcagta 120gctgcctaat cgcactctgc atcgcctaac agcatttcct atgtgctgtt aacgcgattc 180aaccttaata ggatatgcta aacactgccg tttgaagtct gtttagaaga aacttaatca 240aactagcatc atgttggttg tttatcactt ttcatgatgc gaaacctatc gataaactac 300acacgtagaa agatgtgtat caggaccttt ggacgcgggt tcaaatcccg ccgtctccac 360ca362<210> 108<211> 362<212> RNA<213> Staphylococcus aureus<400> 108ggggacguuc auggauucga cagggguccc ccgagcucau uaagcguguc ggaggguugu 60cuucgucauc aacacacaca guuuauaaua acuggcaaau caaacaauaa uuucgcagua 120gcugccuaau cgcacucugc aucgccuaac agcauuuccu augugcuguu aacgcgauuc 180aaccuuaaua ggauaugcua aacacugccg uuugaagucu guuuagaaga aacuuaauca 240aacuagcauc auguugguug uuuaucacuu uucaugaugc gaaaccuauc gauaaacuac 300acacguagaa agauguguau caggaccuuu ggacgcgggu ucaaaucccg ccgucuccac 360ca362<210> 109<211> 349<212> DNA<213> Streptococcus gordonii<400> 109ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcat ctagcggatg 60taaaacgcca gttaaatata actgcaaaaa ataatacttc ttacgcttta gctgcctaaa 120aaccagcggg cgtgacccga ttcggattgc ttgtgtctga tgacaggtct tattattagc 180aagctacggt agaatcttgt ctagtgattt tacaagagat tgatagactc gcttgatttg 240ggcttgagtt atgtgtcaaa atcaagttaa aacaatacat agcctatggt tgtagacaaa 300tgtgttggca gatgtttgga cgtgggttcg actcccaccg gctccacca 349<210> 110<211> 349<212> RNA<213> Streptococcus gordonii<400> 110ggggucguua cggauucgac aggcauuaug aggcauauuu ugcgacucau cuagcggaug 60uaaaacgcca guuaaauaua acugcaaaaa auaauacuuc uuacgcuuua gcugccuaaa 120aaccagcggg cgugacccga uucggauugc uugugucuga ugacaggucu uauuauuagc 180aagcuacggu agaaucuugu cuagugauuu uacaagagau ugauagacuc gcuugauuug 240ggcuugaguu augugucaaa aucaaguuaa aacaauacau agccuauggu uguagacaaa 300uguguuggca gauguuugga cguggguucg acucccaccg gcuccacca 349<210> 111<211> 349<212> DNA<213> Streptococcus mutans<400> 111ggggtcgtta cggattcgac aggcattatg agacctattt tgcgactcat ctagcggatg 60taaaacgcca gttaaatata actgcaaaaa atacaaattc ttacgcagta gctgcctaaa 120aaccagcctg tgtgatcaat aacaaattgc ttgtgtttgt tgattggtct tattgttaac 180aagctacgtt agaactgagt caggctgttc taaaagagtt ctactgactc gcatcgttag 240agtttgagtt atgtattgta acggtgttaa ataaacacat aacctatagt tgtagacaaa 300tgggttagca gatgtttgga cgtgggttcg actcccaccg gctccacca 349<210> 112<211> 349<212> RNA<213> Streptococcus mutans<400> 112ggggucguua cggauucgac aggcauuaug agaccuauuu ugcgacucau cuagcggaug 60uaaaacgcca guuaaauaua acugcaaaaa auacaaauuc uuacgcagua gcugccuaaa 120aaccagccug ugugaucaau aacaaauugc uuguguuugu ugauuggucu uauuguuaac 180aagcuacguu agaacugagu caggcuguuc uaaaagaguu cuacugacuc gcaucguuag 240aguuugaguu auguauugua acgguguuaa auaaacacau aaccuauagu uguagacaaa 300uggguuagca gauguuugga cguggguucg acucccaccg gcuccacca 349<210> 113<211> 348<212> DNA<213> Streptococcus pneumoniae<400> 113ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcgt gtggcgacgt 60aaacgctcag ttaaatataa ctgcaaaaaa taacacttct tacgctctag ctgcctaaaa 120accagcaggc gtgacccgat ttggattgct cgtgttcaat gacaggtctt attattagcg 180agatacgatt aagccttgtc tagcggtttg ataagagatt gatagactcg cagtttctag 240acttgagtta tgtgtcgagg ggctgttaaa ataatacata acctatggtt gtagacaaat 300atgttggcag gtgtttggac gtgggttcga ctcccaccgg ctccacca 348<210> 114<211> 348<212> RNA<213> Streptococcus pneumoniae<400> 114ggggucguua cggauucgac aggcauuaug aggcauauuu ugcgacucgu guggcgacgu 60aaacgcucag uuaaauauaa cugcaaaaaa uaacacuucu uacgcucuag cugccuaaaa 120accagcaggc gugacccgau uuggauugcu cguguucaau gacaggucuu auuauuagcg 180agauacgauu aagccuuguc uagcgguuug auaagagauu gauagacucg caguuucuag 240acuugaguua ugugucgagg ggcuguuaaa auaauacaua accuaugguu guagacaaau 300auguuggcag guguuuggac guggguucga cucccaccgg cuccacca 348<210> 115<211> 348<212> DNA<213> Streptococcus pyogenes<400> 115ggggttgtta cggattcgac aggcattatg aggcatgttt tgcgtcccat cggcagatgt 60aaattgccag ttaaatataa ctgcaaaaaa tacaaactct tacgctttag ctgcctaaaa 120accagctagc gtgacttcta caagattgct tgtgtcctgt tagaagtctc aaaatagcaa 180gctacggtta cgaaattgtc tagtttcgtg acaagagatt gatagactcg caaactaatg 240gcttgagtta tgtgtcttta gtttgttaaa tgaagacata acctatggac gtagacaaat 300atgttggcag gtgtttggac gtgggttcga ctcccaccag ctccacca 348<210> 116<211> 348<212> RNA<213> Streptococcus pyogenes<400> 116gggguuguua cggauucgac aggcauuaug aggcauguuu ugcgucccau cggcagaugu 60aaauugccag uuaaauauaa cugcaaaaaa uacaaacucu uacgcuuuag cugccuaaaa 120accagcuagc gugacuucua caagauugcu uguguccugu uagaagucuc aaaauagcaa 180gcuacgguua cgaaauuguc uaguuucgug acaagagauu gauagacucg caaacuaaug 240gcuugaguua ugugucuuua guuuguuaaa ugaagacaua accuauggac guagacaaau 300auguuggcag guguuuggac guggguucga cucccaccag cuccacca 348<210> 117<211> 394<212> DNA<213> Synechococcus sp. PCC6301<400> 117ggggctgtaa tggtttcgac gtgttggtga atccttcacc gtgattcagg ccgagaggga 60gtccactctc gtaaatccag gctcaaccaa aagtaactgc gaacaacatc gttcctttcg 120ctcgtaaggc tgctcctgta gctgcttaaa cgccacaaac tttctggctc gagcgtctag 180tcgtagactc cgttaatacg cctagactta aacccccaac ggatgctcga gtggcggcct 240caggtccgtc ctctcgctaa gcaaaaacct gagcatcccg ccaacgggga taatcgttgg 300ctcccgcaca gtgggtcaac cgtgctaagc ctgtgaacga gcggaaagtt actagtcaat 360gcggacagcg gttcgattcc gctcagctcc acca 394<210> 118<211> 394<212> RNA<213> Synechococcus sp. PCC6301<400> 118ggggcuguaa ugguuucgac guguugguga auccuucacc gugauucagg ccgagaggga 60guccacucuc guaaauccag gcucaaccaa aaguaacugc gaacaacauc guuccuuucg 120cucguaaggc ugcuccugua gcugcuuaaa cgccacaaac uuucuggcuc gagcgucuag 180ucguagacuc cguuaauacg ccuagacuua aacccccaac ggaugcucga guggcggccu 240cagguccguc cucucgcuaa gcaaaaaccu gagcaucccg ccaacgggga uaaucguugg 300cucccgcaca gugggucaac cgugcuaagc cugugaacga gcggaaaguu acuagucaau 360gcggacagcg guucgauucc gcucagcucc acca 394<210> 119<211> 399<212> DNA<213> Synechocystis PCC6803<400> 119ggggccgcaa tggtttcgac aggttggcga aagcttgccc gtgatacagg tcgagagtga 60gtctcctctc gcaaatcaaa ggctcaaaaa aaagtaactg cgaataacat cgtcagcttc 120aaacgggtag ccatagcagc ctagtctgta aaagctacat tttcttgtca aagaccgttt 180acttcttttc tgactccgtt aaggattaga ggttaacccc aacggatgct ttgtttggct 240cttctctagt tagctaaaca atcaagactc agactagagc atcccaccat cagggataat 300cgatggtccc cgtcctaggg ctagaaggac taaacctgtg aatgagcgga aagttaatac 360ccagtttgga cagcagttca attctgctcg gctccacca399<210> 120<211> 399<212> RNA<213> Synechocystis PCC6803<400> 120ggggccgcaa ugguuucgac agguuggcga aagcuugccc gugauacagg ucgagaguga 60gucuccucuc gcaaaucaaa ggcucaaaaa aaaguaacug cgaauaacau cgucagcuuc 120aaacggguag ccauagcagc cuagucugua aaagcuacau uuucuuguca aagaccguuu 180acuucuuuuc ugacuccguu aaggauuaga gguuaacccc aacggaugcu uuguuuggcu 240cuucucuagu uagcuaaaca aucaagacuc agacuagagc aucccaccau cagggauaau 300cgaugguccc cguccuaggg cuagaaggac uaaaccugug aaugagcgga aaguuaauac 360ccaguuugga cagcaguuca auucugcucg gcuccacca399<210> 121<211> 356<212> DNA<213> Thermotoga maritima<400> 121gggggcgaac gggttcgacg gggatggagt cccctgggaa gcgagccgag gtccccacct 60cctcgtaaaa aaggtgggac aaagaataag tgccaacgaa cctgttgctg ttgccgctta 120atagataagc ggccgtcctc tccgaagttg gctgggcttc ggaagagggc gtgagagatc 180cagcctaccg attcagcttc gccttccggc ctgaatcggg aaaactcagg aaggctgtgg 240gagaggacac cctgcccgtg ggaggtccct cccgagagcg aaaacacggg ctgcgctcgg 300agaagcccag gggcctccat cttcggacgg gggttcgaat ccccccgcct ccacca 356<210> 122<211> 356<212> RNA<213> Thermotoga maritima<400> 122gggggcgaac ggguucgacg gggauggagu ccccugggaa gcgagccgag guccccaccu 60ccucguaaaa aaggugggac aaagaauaag ugccaacgaa ccuguugcug uugccgcuua 120auagauaagc ggccguccuc uccgaaguug gcugggcuuc ggaagagggc gugagagauc 180cagccuaccg auucagcuuc gccuuccggc cugaaucggg aaaacucagg aaggcugugg 240gagaggacac ccugcccgug ggaggucccu cccgagagcg aaaacacggg cugcgcucgg 300agaagcccag gggccuccau cuucggacgg ggguucgaau ccccccgccu ccacca 356<210> 123<211> 349<212> DNA<213> Thermus thermophilus<400> 123gggggtgaaa cggtctcgac gggggtcgcc gagggcgtgg ctgcgcgccg aggtgcgggt 60ggcctcgtaa aaacccgcaa cggcataact gccaacacca actacgctct cgcggcttaa 120tgaccgcgac ctcgcccggt agccctgccg ggggctcacc ggaagcgggg acacaaaccc 180ggctagcccg gggccacgcc ctctaacccc gggcgaagct tgaagggggc tcgctcctgg 240ccgcccgtcc gcgggccaag ccaggaggac acgcgaaacg cggactacgc gcgtagaggc 300ccgccgtaga gaccttcgga cgggggttcg actcccccca cctccacca 349<210> 124<211> 349<212> RNA<213> Thermus thermophilus<400> 124gggggugaaa cggucucgac gggggucgcc gagggcgugg cugcgcgccg aggugcgggu 60ggccucguaa aaacccgcaa cggcauaacu gccaacacca acuacgcucu cgcggcuuaa 120ugaccgcgac cucgcccggu agcccugccg ggggcucacc ggaagcgggg acacaaaccc 180ggcuagcccg gggccacgcc cucuaacccc gggcgaagcu ugaagggggc ucgcuccugg 240ccgcccgucc gcgggccaag ccaggaggac acgcgaaacg cggacuacgc gcguagaggc 300ccgccguaga gaccuucgga cggggguucg acucccccca ccuccacca 349<210> 125<211> 354<212> DNA<213> Treponema pallidum<400> 125ggggatgact aggtttcgac tagggatgtg gggtgttgcg ctgcaggtgg agtgtcgatc 60tcctgattcg gcgcctttat aactgccaat tctgacagtt tcgactacgc gctcgccgcg 120taatcgcggg cctgtgtttg cgctgctctg agcgaacata tcggcccgac gccaaacgga 180gcttgctctt acgttgtgca cggcggacgt agggggactt ttgtctgtgc taagactctg 240gcgcgtgcgg tgcaggccta gcagagtccg acaaacgcag tacgcaccgc taaacctgta 300ggcgcgcagc actcgctctt taggacgggg gttcgattcc ccccatctcc acca 354<210> 126<211> 354<212> RNA<213> Treponema pallidum<400> 126ggggaugacu agguuucgac uagggaugug ggguguugcg cugcaggugg agugucgauc 60uccugauucg gcgccuuuau aacugccaau ucugacaguu ucgacuacgc gcucgccgcg 120uaaucgcggg ccuguguuug cgcugcucug agcgaacaua ucggcccgac gccaaacgga 180gcuugcucuu acguugugca cggcggacgu agggggacuu uugucugugc uaagacucug 240gcgcgugcgg ugcaggccua gcagaguccg acaaacgcag uacgcaccgc uaaaccugua 300ggcgcgcagc acucgcucuu uaggacgggg guucgauucc ccccaucucc acca 354<210> 127<211> 367<212> DNA<213> Vibrio cholerae<400> 127ggggctgatt caggattcga cgggaatttt gcagtctgag gtgcatgccg aggtgcggta 60ggcctcgtta acaaaccgca aaaaaatagt cgcaaacgac gaaaactacg cactagcagc 120ttaataccct gctcagagcc cttcctccct agcttccgct tgtaagacgg ggaaatcagg 180aaggtcaaac caaatcaagc tggcgtggat tcccccacct gagggatgaa gcgcgagatc 240taattcaggt tagccattcg ttagcgtgtc ggttcgcagg cggtggtgaa attaaagatc 300gactaagcat gtagtaccaa agatgaatgg ttttcggacg ggggttcaac tccccccagc 360tccacca 367<210> 128<211> 367<212> RNA<213> Vibrio cholerae<400> 128ggggcugauu caggauucga cgggaauuuu gcagucugag gugcaugccg aggugcggua 60ggccucguua acaaaccgca aaaaaauagu cgcaaacgac gaaaacuacg cacuagcagc 120uuaauacccu gcucagagcc cuuccucccu agcuuccgcu uguaagacgg ggaaaucagg 180aaggucaaac caaaucaagc uggcguggau ucccccaccu gagggaugaa gcgcgagauc 240uaauucaggu uagccauucg uuagcguguc gguucgcagg cgguggugaa auuaaagauc 300gacuaagcau guaguaccaa agaugaaugg uuuucggacg gggguucaac uccccccagc 360uccacca 367<210> 129<211> 364<212> DNA<213> Yersinia pestis<400> 129ggggctgatt ctggattcga cgggattcgc gaaacccaag gtgcatgccg aggtgcggtg 60gcctcgtaaa aaaccgcaaa aaaaatagtt gcaaacgacg aaaactacgc actagcagct 120taataacctg cttagagccc tctctgccta gcctccgctc ttaggacggg gatcaagaga 180ggtcaaacct aaaagagctc gtgtggaaac cttgcctggg gtggaagcat taaaactaat 240caggatagtt tgtcagtagc gtgtccatcc gcagctggcc ggcgaatgta atgattggac 300taagcatgta gtgccgacgg tgtagtaatt tcggacgggg gttcaaatcc ccccagctcc 360acca 364<210> 130<211> 364<212> RNA<213> Yersinia pestis<400> 130ggggcugauu cuggauucga cgggauucgc gaaacccaag gugcaugccg aggugcggug 60gccucguaaa aaaccgcaaa aaaaauaguu gcaaacgacg aaaacuacgc acuagcagcu 120uaauaaccug cuuagagccc ucucugccua gccuccgcuc uuaggacggg gaucaagaga 180ggucaaaccu aaaagagcuc guguggaaac cuugccuggg guggaagcau uaaaacuaau 240caggauaguu ugucaguagc guguccaucc gcagcuggcc ggcgaaugua augauuggac 300uaagcaugua gugccgacgg uguaguaauu ucggacgggg guucaaaucc ccccagcucc 360acca 364<210> 131<211> 309<212> DNA<213> Campylobacter fetus<400> 131aggagtaagt ctgcttagat ggcatgtcgc tttgggcaaa gcgtaaaaag cccaaataaa 60attaaacgca aacaacgtta aattcgctcc tgcttacgct aaagctgcgt aagttcagtt 120gagcctgaaa tttaagtcat actatctagc ttaattttcg gtcatctttg atagtgtagc 180cttgcgtttg acaagcgttg aggtgaaata aagtcttagc cttgcttttg agttttggaa 240gatgagcgaa gtagggtgaa gtagtcatct ttgctaagca tgtagaggtc tttgtgggat 300tatttttgg 309<210> 132<211> 309<212> RNA<213> Campylobacter fetus<400> 132aggaguaagu cugcuuagau ggcaugucgc uuugggcaaa gcguaaaaag cccaaauaaa 60auuaaacgca aacaacguua aauucgcucc ugcuuacgcu aaagcugcgu aaguucaguu 120gagccugaaa uuuaagucau acuaucuagc uuaauuuucg gucaucuuug auaguguagc 180cuugcguuug acaagcguug aggugaaaua aagucuuagc cuugcuuuug aguuuuggaa 240gaugagcgaa guagggugaa guagucaucu uugcuaagca uguagagguc uuugugggau 300uauuuuugg 309<210> 133<211> 309<212> DNA<213> Campylobacter coli (BM2509)<400> 133aggagtaagt ctgcttagat ggcatgtcgc tttggacaaa gcgtaaaaag tccaaattaa 60aattaaacgc aaataacgtt aaatttgctc ctgcttacgc taaagctgcg taagttcagt 120tgagcccgaa actcaagtga tgctatctag cttgaatttt ggtcatcttt gatagtgtag 180attgaaaatt gacaactttt aatcgaagtt aaagtcttag tctagcttga aattttggaa 240ggtgagttta gccagatgaa gttttcacct ttgctaaaca tgtagaagtc tttgtggggt 300tatttttgg 309<210> 134<211> 309<212> RNA<213> Campylobacter coli (BM2509)<400> 134aggaguaagu cugcuuagau ggcaugucgc uuuggacaaa gcguaaaaag uccaaauuaa 60aauuaaacgc aaauaacguu aaauuugcuc cugcuuacgc uaaagcugcg uaaguucagu 120ugagcccgaa acucaaguga ugcuaucuag cuugaauuuu ggucaucuuu gauaguguag 180auugaaaauu gacaacuuuu aaucgaaguu aaagucuuag ucuagcuuga aauuuuggaa 240ggugaguuua gccagaugaa guuuucaccu uugcuaaaca uguagaaguc uuuguggggu 300uauuuuugg 309<210> 135<211> 311<212> DNA<213> 未知<220><223> 未知生物體的描述Campylobacter chicken分離物<400> 135acaggagtaa gtctgcttag atggcatgtc gctttgggca aagcgtaaaa agcccaaata 60aaattaaacg caaacaacgt taaattcgct cctgcttacg ctaaagctgc gtaagttcag 120ttgagcctga aatttaagtc atactatcta gcttaatttt cggtcatttt tgatagtgta 180gccttgcgtt tgacaagcgt tgaggtgaaa taaggtctta gccttgcttt tgagttttgg 240aagatgagcg aagtagggtg aagtagtcat ctttgctaag catgtagagg tctttgtggg 300attatttttg g 311<210> 136<211> 311<212> RNA<213> 未知<220><223> 未知生物體的描述Campylobacter chicken分離物<400> 136acaggaguaa gucugcuuag auggcauguc gcuuugggca aagcguaaaa agcccaaaua 60aaauuaaacg caaacaacgu uaaauucgcu ccugcuuacg cuaaagcugc guaaguucag 120uugagccuga aauuuaaguc auacuaucua gcuuaauuuu cggucauuuu ugauagugua 180gccuugcguu ugacaagcgu ugaggugaaa uaaggucuua gccuugcuuu ugaguuuugg 240aagaugagcg aaguagggug aaguagucau cuuugcuaag cauguagagg ucuuuguggg 300auuauuuuug g 311<210> 137<211> 313<212> DNA<213> Clostridium perfringens<400> 137acgggggtag gatgggtttg ataagcgagt cgagggaagc atggtgcctc gataataaag 60tatgcattaa agataaacgc acgagataat tttgcattag cagcttaagt tagcgctgct 120catccttcct caattgccca cggttgagag taagggtgtc atttaaaagt ggggaaccga 180gcctagcaaa gctttgagct aggaacggaa tttatgaagc ttaccaaaga ggaagtttgt 240ctgtggacgt tctctgaggg aattttaaaa cacaagacta cactcgtaga aagtcttact 300ggtctgcttt cgg313<210> 138<211> 313<212> RNA<213> Clostridium perfringens<400> 138acggggguag gauggguuug auaagcgagu cgagggaagc auggugccuc gauaauaaag 60uaugcauuaa agauaaacgc acgagauaau uuugcauuag cagcuuaagu uagcgcugcu 120cauccuuccu caauugccca cgguugagag uaaggguguc auuuaaaagu ggggaaccga 180gccuagcaaa gcuuugagcu aggaacggaa uuuaugaagc uuaccaaaga ggaaguuugu 240cuguggacgu ucucugaggg aauuuuaaaa cacaagacua cacucguaga aagucuuacu 300ggucugcuuu cgg313<210> 139<211> 331<212> DNA<213> Haemophilus ducreyi (NCTC 10945)<400> 139acgggattag cgaagtccaa ggtgcacgtc gaggtgcggt aggcctcgta acaaaccgca 60aaaaaatagt cgcaaacgac gaacaatacg ctttagcagc ttaataacct gcatttagcc 120ttcgcgccct agctttcgct cgtaagacgg ggagcacgcg gagtcaaacc aaaacgagat 180cgtgtggacg cttccgcttg tagatgaaac actaaattga atcaagctag tttatttctt 240gcgtgtctgt ccgctggaga taagcgaaat taaagaccag actaaacgtg tagtactgaa 300gatagagtaa tttcggaccc gggttcgact c331<210> 140<211> 331<212> RNA<213> Haemophilus ducreyi (NCTC 10945)<400> 140acgggauuag cgaaguccaa ggugcacguc gaggugcggu aggccucgua acaaaccgca 60aaaaaauagu cgcaaacgac gaacaauacg cuuuagcagc uuaauaaccu gcauuuagcc 120uucgcgcccu agcuuucgcu cguaagacgg ggagcacgcg gagucaaacc aaaacgagau 180cguguggacg cuuccgcuug uagaugaaac acuaaauuga aucaagcuag uuuauuucuu 240gcgugucugu ccgcuggaga uaagcgaaau uaaagaccag acuaaacgug uaguacugaa 300gauagaguaa uuucggaccc ggguucgacu c331<210> 141<211> 232<212> DNA<213> Listeria innocua(食物分離物#1)<400> 141ggcaaagaaa aacaaaacct agctttcgct gcctaataac cagtagcata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctgag gttaaataga agagcttaat cagactagct gaatggaagc ctgttaccgg 180gctgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattc aa 232<210> 142<211> 232<212> RNA<213>Listeria innocua(食物分離物#1)<400> 142ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaac caguagcaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucugag guuaaauaga agagcuuaau cagacuagcu gaauggaagc cuguuaccgg 180gcugauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuc aa 232<210> 143<211> 232<212> DNA<213> Listeria innocua(食物分離物#2)<400> 143ggcaaagaaa aacaaaacct agctttcgct gcctaataag cagtagcata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctgag gttaaataga agagcttaat cagactagct gaatggaagc ctgttaccgg 180gccgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattt aa 232<210> 144<211> 232<212> RNA<213>Listeria innocua(食物分離物#2)<400> 144ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaag caguagcaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucugag guuaaauaga agagcuuaau cagacuagcu gaauggaagc cuguuaccgg 180gccgauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuu aa 232<210> 145<211> 232<212> DNA<213> Listeria innocua(食物分離物#3)<400> 145ggcaaagaaa aacaaaacct agctttcgct gcctaataag cagtagaata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctgag gttaaataga agagcttaat cggactagct gaatggaagc ctgttaccgg 180gccgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattt aa 232<210> 146<211> 232<212> RNA<213> Listeria innocua(食物分離物#3)<400> 146ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaag caguagaaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucugag guuaaauaga agagcuuaau cggacuagcu gaauggaagc cuguuaccgg 180gccgauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuu aa 232<210> 147<211> 232<212> DNA<213> Listeria innocua (ATCC 12210)<400> 147ggcaaagaaa aacaaaacct agctttcgct gcctaataag cagtagcata gctgatcctc 60cgtgcatcgc ccatgtgcta cggtaagggt ctcactctaa gtgggctaca ctagttaatc 120tccgtctggg gttaaataga agagcttaat cagactagct gaatggaagc ctgttactgg 180gccgatgttt atgcgaaatg ctaatacggt gactacgctc gtagatattt aa 232<210> 148<211> 232<212> RNA<213> Listeria innocua (ATCC 12210)<400> 148ggcaaagaaa aacaaaaccu agcuuucgcu gccuaauaag caguagcaua gcugauccuc 60cgugcaucgc ccaugugcua cgguaagggu cucacucuaa gugggcuaca cuaguuaauc 120uccgucuggg guuaaauaga agagcuuaau cagacuagcu gaauggaagc cuguuacugg 180gccgauguuu augcgaaaug cuaauacggu gacuacgcuc guagauauuu aa 232<210> 149<211> 322<212> DNA<213> Listeria ivanovii (NCTC 11846)<400> 149acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta ttaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg 322<210> 150<211> 321<212> RNA<213> Listeria ivanovii (NCTC 11846)<400> 150acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua uuaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacucg cucguagaua 300uuuaagugcc gauauuucug g 321<210> 151<211> 322<212> DNA<213> Listeria seeligeri (NCTC 11856)<400> 151acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggaaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatactaata cggtgactac gctcgtagat 300atttaagtgc ccatatttct gg 322<210> 152<211> 322<212> RNA<213> Listeria seeligeri (NCTC 11856)<400> 152acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacggaaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aauacuaaua cggugacuac gcucguagau 300auuuaagugc ccauauuucu gg 322<210> 153<211> 314<212> DNA<213> Salmonella enteritidis<400> 153acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca 60aaaaaatagt cgcaaacgac gaaacctacg ctttagcagc ttaataacct gcttagagcc 120ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat 180cgcgtggatg ccctgcctgg ggttgaagcg ttaaaacgaa tcaggctagt ctggtagtgg 240cgtgtccgtc cgcaggtgcc aggcgaatgt aaagactgac taagcatgta gtaccgagga 300tgtaggaatt tcgg 314<210> 154<211> 314<212> RNA<213> Salmonella enteritidis<400> 154acgggauuug cgaaacccaa ggugcaugcc gaggggcggu uggccucgua aaaagccgca 60aaaaaauagu cgcaaacgac gaaaccuacg cuuuagcagc uuaauaaccu gcuuagagcc 120cucucucccu agccuccgcu cuuaggacgg ggaucaagag aggucaaacc caaaagagau 180cgcguggaug cccugccugg gguugaagcg uuaaaacgaa ucaggcuagu cugguagugg 240cguguccguc cgcaggugcc aggcgaaugu aaagacugac uaagcaugua guaccgagga 300uguaggaauu ucgg 314<210> 155<211> 313<212> DNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 155acaggggtcc cccgagctta ttaagcgtgt cggagggttg gctccgtcat caacacattt 60cggttaaata taactgacaa atcaaacaat aatttcgcag tagctgcgta atagccactg 120catcgcctaa cagcatctcc tacgtgctgt taacgcgatt caaccctagt aggatatgct 180aaacactgcc gcttgaagtc tgtttagatg aaatataatc aagctagtat catgttggtt 240gtttattgct tagcatgatg cgaaaattat caataaacta cacacgtaga aagatttgta 300tcaggacctc tgg313<210> 156<211> 313<212> RNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 156acaggggucc cccgagcuua uuaagcgugu cggaggguug gcuccgucau caacacauuu 60cgguuaaaua uaacugacaa aucaaacaau aauuucgcag uagcugcgua auagccacug 120caucgccuaa cagcaucucc uacgugcugu uaacgcgauu caacccuagu aggauaugcu 180aaacacugcc gcuugaaguc uguuuagaug aaauauaauc aagcuaguau cauguugguu 240guuuauugcu uagcaugaug cgaaaauuau caauaaacua cacacguaga aagauuugua 300ucaggaccuc ugg313<210> 157<211> 302<212> DNA<213> Streptococcus agalactiae (NCTC 8181)<400> 157acaggcatta tgaggtatat tttgcgactc atcggcagat gtaaaatgcc agttaaatat 60aactgcaaaa aatacaaatt cttacgcatt agctgcctaa aaaacagcct gcgtgatctt 120cacaagattg tttgcgtttt gctagaaggt cttatttatc agcaaactac gtttggctac 180tgtctagtta gttaaaaaga gatttataga ctcgctatgt gagggcttga gttatgtgtc 240atcacctagt taaatcaata cataacctat agttgtagac aaatatatta gcagatgttt 300gg302<210> 158<211> 302<212> RNA<213> Streptococcus agalactiae (NCTC 8181)<400> 158acaggcauua ugagguauau uuugcgacuc aucggcagau guaaaaugcc aguuaaauau 60aacugcaaaa aauacaaauu cuuacgcauu agcugccuaa aaaacagccu gcgugaucuu 120cacaagauug uuugcguuuu gcuagaaggu cuuauuuauc agcaaacuac guuuggcuac 180ugucuaguua guuaaaaaga gauuuauaga cucgcuaugu gagggcuuga guuauguguc 240aucaccuagu uaaaucaaua cauaaccuau aguuguagac aaauauauua gcagauguuu 300gg302<210> 159<211> 168<212> DNA<213> Bordetella bronchiseptica<400> 159ggggccgatc cggattcgac gtgggtcatg aaacagctca aggcatgccg agcaccagta 60agctcgttaa tccactggaa cactacaaac gccaacgacg agcgtttcgc tctcgccgct 120taagcggtga gccgctgcac tgatctgtcc ttgggtcacg cgggggaa 168<210> 160<211> 168<212> RNA<213> Bordetella bronchiseptica<400> 160ggggccgauc cggauucgac gugggucaug aaacagcuca aggcaugccg agcaccagua 60agcucguuaa uccacuggaa cacuacaaac gccaacgacg agcguuucgc ucucgccgcu 120uaagcgguga gccgcugcac ugaucugucc uugggucacg cgggggaa 168<210> 161<211> 426<212> DNA<213> Chlamydia pneumoniae (CWL029)<400> 161gggggtgtat aggtttcgac ttgaaaatga agtgttaatt gcatgcggag ggcgttggct 60ggcctcctaa aaagccaaca aaacaataaa tgccgaacct aaggctgaat gcgaaattat 120tagcttgttt gactcagtag aggaaagact agctgcttaa ttagcaaaag ttgttagcta 180gataatctct aggtaacccg gtatctgcga gctccaccag aggcttgcaa aataccgtca 240tttatctggt tggaacttac tttctctaat tctcaaggaa gttcgttcga gatttttgag 300agtcattggc tgctatagag gcttctagct aagggagtcc aatgtaaaca attctagaag 360ataagcatgt agaggttagc agggagtttg tcaaggacga gagttcgagt ctctccacct 420ccacca426<210> 162<211> 426<212> RNA<213> Chlamydia pneumoniae (CWL029)<400> 162ggggguguau agguuucgac uugaaaauga aguguuaauu gcaugcggag ggcguuggcu 60ggccuccuaa aaagccaaca aaacaauaaa ugccgaaccu aaggcugaau gcgaaauuau 120uagcuuguuu gacucaguag aggaaagacu agcugcuuaa uuagcaaaag uuguuagcua 180gauaaucucu agguaacccg guaucugcga gcuccaccag aggcuugcaa aauaccguca 240uuuaucuggu uggaacuuac uuucucuaau ucucaaggaa guucguucga gauuuuugag 300agucauuggc ugcuauagag gcuucuagcu aagggagucc aauguaaaca auucuagaag 360auaagcaugu agagguuagc agggaguuug ucaaggacga gaguucgagu cucuccaccu 420ccacca426<210> 163<211> 421<212> DNA<213> Francisella tularensis<400> 163gggggcgaat atggtttcga catgaatgtc aaaatctaag gtgcatgccg aggaagtacc 60gtaacctcgt taataacagt acaaatgcca ataataactg gcaacaaaaa agcaaaccgc 120gtagcggcta acgacagcaa ctttgctgct gttgctaaag ctgcctagtc tagcttaata 180atctagatgc gcacggatat gatagtcttt cttatgacac tatctataca tccgttcata 240ttccgcataa gacggtcttt gctttttgtc tgggagttaa ggctgtattt aacagactcg 300ctaactatta ccctggctaa ttggggaata gtcaagctaa actcaaatag attagcctaa 360gcatgtagat ccaaagatct agagtttgtg gacgcgggtt caaatcccgc cgcctccacc 420a 421<210> 164<211> 421<212> RNA<213> Francisella tularensis<400> 164gggggcgaau augguuucga caugaauguc aaaaucuaag gugcaugccg aggaaguacc 60guaaccucgu uaauaacagu acaaaugcca auaauaacug gcaacaaaaa agcaaaccgc 120guagcggcua acgacagcaa cuuugcugcu guugcuaaag cugccuaguc uagcuuaaua 180aucuagaugc gcacggauau gauagucuuu cuuaugacac uaucuauaca uccguucaua 240uuccgcauaa gacggucuuu gcuuuuuguc ugggaguuaa ggcuguauuu aacagacucg 300cuaacuauua cccuggcuaa uuggggaaua gucaagcuaa acucaaauag auuagccuaa 360gcauguagau ccaaagaucu agaguuugug gacgcggguu caaaucccgc cgccuccacc 420a 421<210> 165<211> 330<212> DNA<213> Guillardia theta (質(zhì)體)<400> 165ggggctgatt tggattcgac atataaattt gcgtgtttca ttatgaagca agtcaagttt 60aatgatcttg taaaaaacat taaagtacaa ataaatgcaa gcaatatagt ttcatttagt 120tcaaaacgtt tagtctcttt tgcataagca aaatgtgtta ataactttct tagtagaaat 180tggagaagtt tactaagatt tatatttact ccataattat tttaaagatg gtaaaaaggt 240gattcatcat ttgtatgttt ctaaactttg tgaaagaata gtgggctcca tttataatga 300acgtgggttc aaatcccacc agctccacca 330<210> 166<211> 330<212> RNA<213> Guillardia theta(質(zhì)體)<400> 166ggggcugauu uggauucgac auauaaauuu gcguguuuca uuaugaagca agucaaguuu 60aaugaucuug uaaaaaacau uaaaguacaa auaaaugcaa gcaauauagu uucauuuagu 120ucaaaacguu uagucucuuu ugcauaagca aaauguguua auaacuuucu uaguagaaau 180uggagaaguu uacuaagauu uauauuuacu ccauaauuau uuuaaagaug guaaaaaggu 240gauucaucau uuguauguuu cuaaacuuug ugaaagaaua gugggcucca uuuauaauga 300acguggguuc aaaucccacc agcuccacca 330<210> 167<211> 348<212> DNA<213> Thalassiosira Weissflogii(質(zhì)體)<400> 167ggggctgatt tggtttcgac atttaaaact tctttctatg tgtcaggtca aagtttgtat 60tctttgtaaa aaaatactaa aatactaata aatgctaata atataatacc gtttattttt 120aaagcagtaa aaacaaaaaa agaagcaatg gctttaaatt ttgctgtata gttcattaac 180ttaggttatt aaatattttt tcattataac tggacttttc tctagtttat agtttagaat 240aaatttaaat tttgcaaaac tcgttcgaaa attttcgggc taaacctgta aacgcaaata 300ctaagaaatt ttagatggac atgggttcaa ttcccatcag ttccacca 348<210> 168<211> 348<212> RNA<213> Thalassiosira Weissflogii(質(zhì)體)<400> 168ggggcugauu ugguuucgac auuuaaaacu ucuuucuaug ugucagguca aaguuuguau 60ucuuuguaaa aaaauacuaa aauacuaaua aaugcuaaua auauaauacc guuuauuuuu 120aaagcaguaa aaacaaaaaa agaagcaaug gcuuuaaauu uugcuguaua guucauuaac 180uuagguuauu aaauauuuuu ucauuauaac uggacuuuuc ucuaguuuau aguuuagaau 240aaauuuaaau uuugcaaaac ucguucgaaa auuuucgggc uaaaccugua aacgcaaaua 300cuaagaaauu uuagauggac auggguucaa uucccaucag uuccacca 348<210> 169<211> 19<212> DNA<213> 人工序列<220><223> 人工序列的描述V.cholerae tmRNA特異性探針<400> 169aacgaatggc taacctgaa 19<210> 170<211> 17<212> DNA<213> 人工序列<220><223> 人工序列的描述通用ssrA/tmRNA 5’體外擴增引物<400> 170gggmytacgg wttcgac17<210> 171<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述通用ssrA/tmRNA 3’體外擴增引物<400> 171gggartcgaa ccrsgtcc 18<210> 172<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述5’Listeria屬特異性PCR擴增引物<400> 172aaagccaata ataactgg 18<210> 173<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述3’Listeria屬特異性擴增引物<400> 173ccagaaatat cggcactt 18<210> 174<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述Listeria屬特異性雜交探針<400> 174gtgagaccct taccgtag 18<210> 175<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述Listeria monocytogenes種特異性雜交探針<400> 175tctatttaac cccagacg 18<210> 176<211> 348<212> DNA<213> Helicobacter pylori<400> 176tggggatgtt acggtttcga caggggtagt tcgagcttag gtggcgagtc gaggggatcg 60gcctcgttaa aacgtcaaag cctataactg gcaaacaaca aaacaacttc gctttagcag 120cttaataagc tcttagcggt tcctccctcc atcgcccatg tggtagggta agggactcaa 180attaagtggg ctacgctgga ttccaccgtc tgaggatgaa agaagagaac aaccagacta 240gctacccgga cgcccgtcga taggcagatg gagtagcgaa tcgcgaatat atcgactaca 300ctcgtagaag cttaagtgcc gatattcttg gacgtgggtt cgactccc 348<210> 177<211> 348<212> RNA<213> Helicobacter pylori<400> 177uggggauguu acgguuucga cagggguagu ucgagcuuag guggcgaguc gaggggaucg 60gccucguuaa aacgucaaag ccuauaacug gcaaacaaca aaacaacuuc gcuuuagcag 120cuuaauaagc ucuuagcggu uccucccucc aucgcccaug ugguagggua agggacucaa 180auuaaguggg cuacgcugga uuccaccguc ugaggaugaa agaagagaac aaccagacua 240gcuacccgga cgcccgucga uaggcagaug gaguagcgaa ucgcgaauau aucgacuaca 300cucguagaag cuuaagugcc gauauucuug gacguggguu cgacuccc 348<210> 178<211> 344<212> DNA<213> Helicobacter pylori<400> 178tggggacgtt acggtttcga cagggatagt tcgagcttag gttgcgagtc gaggggatcg 60gcctcgttaa aacgtcaaag cctataattg gcaaacaaaa caatctttct ttagctgctt 120aattgcacta aaggttcctc cctccatcgt ccatgtggta gggtaaggga ctcaaactaa 180gtggactacg ccggagttcg ccgtctgagg acaaaggaag agaacaacca gactagcaac 240ttggaagcct gtcgataggc cgaagagttc gcgaaatgct aatatatcga ctacactcgt 300agaagcttaa gtgccgatat ttttggacgt gggttcgatt ccct 344<210> 179<211> 344<212> RNA<213> Helicobacter pylori<400> 179uggggacguu acgguuucga cagggauagu ucgagcuuag guugcgaguc gaggggaucg 60gccucguuaa aacgucaaag ccuauaauug gcaaacaaaa caaucuuucu uuagcugcuu 120aauugcacua aagguuccuc ccuccaucgu ccauguggua ggguaaggga cucaaacuaa 180guggacuacg ccggaguucg ccgucugagg acaaaggaag agaacaacca gacuagcaac 240uuggaagccu gucgauaggc cgaagaguuc gcgaaaugcu aauauaucga cuacacucgu 300agaagcuuaa gugccgauau uuuuggacgu ggguucgauu cccu 344<210> 180<211> 322<212> DNA<213> Listeria seeligeri(NCTC 11856)<400> 180acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta tcaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggaaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatactaata cggtgactac gctcgtagat 300atttaagtgc ccatatttct gg 322<210> 181<211> 322<212> RNA<213> Listeria seeligeri (NCTC 11856)<400> 181acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua ucaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacggaaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aauacuaaua cggugacuac gcucguagau 300auuuaagugc ccauauuucu gg 322<210> 182<211> 322<212> DNA<213> Listeria ivanovii (NCTC 11846)<400> 182acagggatag ttcgagcttg agttgcgagt cggggggatc gtcctcgtta ttaacgtcaa 60agccaataat aactggcaaa gaaaaacaaa acctagcttt cgctgcctaa taagcagtag 120catagctgat cctccgtgca tcgcccatgt gctacggtaa gggtctcact ttaagtgggc 180tacactaaat aatctccgtc tggggttagt tagaagagct taatcagact agctgaatgg 240aagcctgtta ccgggctgat gtttatgcga aatgctaata cggtgactac gctcgtagat 300atttaagtgc cgatatttct gg 322<210> 183<211> 321<212> RNA<213> Listeria ivanovii (NCTC 11846)<400> 183acagggauag uucgagcuug aguugcgagu cggggggauc guccucguua uuaacgucaa 60agccaauaau aacuggcaaa gaaaaacaaa accuagcuuu cgcugccuaa uaagcaguag 120cauagcugau ccuccgugca ucgcccaugu gcuacgguaa gggucucacu uuaagugggc 180uacacuaaau aaucuccguc ugggguuagu uagaagagcu uaaucagacu agcugaaugg 240aagccuguua ccgggcugau guuuaugcga aaugcuaaua cggugacucg cucguagaua 300uuuaagugcc gauauuucug g 321<210> 184<211> 319<212> DNA<213> Mycobacterium africanum<400> 184acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcgacca aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcagaccgg gaacgccctc ggcccggacc ctggcatcag ctagagggat 180ccaccgatga gtccggtcgc gggactcctc gggacaacca cagcgactgg gatcgtcatc 240tcggctagtt cgcgtgaccg ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgta 319<210> 185<211> 319<212> RNA<213> Mycobacterium africanum<400> 185acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcgacca aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucagaccgg gaacgcccuc ggcccggacc cuggcaucag cuagagggau 180ccaccgauga guccggucgc gggacuccuc gggacaacca cagcgacugg gaucgucauc 240ucggcuaguu cgcgugaccg ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccgua 319<210> 186<211> 319<212> DNA<213> Mycobacterium gordonae<400> 186acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca tataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcggaccgg gaacgccctc gccccggacc ccggcatcag ctagagggat 180caaccgatga gttcggtcgc gggactcatc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtc cgtgtgacca ggagatccga gcagagacat agcggactgc gcacggagaa 300gccttgaggg aatgccgta 319<210> 187<211> 319<212> RNA<213> Mycobacterium gordonae<400> 187acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca uauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucggaccgg gaacgcccuc gccccggacc ccggcaucag cuagagggau 180caaccgauga guucggucgc gggacucauc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguc cgugugacca ggagauccga gcagagacau agcggacugc gcacggagaa 300gccuugaggg aaugccgua 319<210> 188<211> 319<212> DNA<213> Mycobacterium kansasii<400> 188acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcagaccgg gaccgccctc gacccggact ctggcatcag ctagagggat 180caaccgatga gttcggtcgc gggactcgtc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtt cgcgtgacca ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgta 319<210> 189<211> 319<212> RNA<213> Mycobacterium kansasii<400> 189acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucagaccgg gaccgcccuc gacccggacu cuggcaucag cuagagggau 180caaccgauga guucggucgc gggacucguc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguu cgcgugacca ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccgua 319<210> 190<211> 320<212> DNA<213> Mycobacterium chelonae<400> 190acagcgagtc tcgacttaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60attgcaacca attaagcgcc gattctcatc agcgcgacta cgcactcgct gcctaagcga 120ctgcgtgtct gtcagaccgg gagcgccctc agcccggacc ctggcatcag ctagagggac 180aaactacggg ttcggtcgcg ggacccgtag ggacatcaaa cagcgactgg gatcgtcatc 240tcggcttgtt cgcgggaccg agagatccaa gtagaggcat agcgaactgc gcacggagaa 300gccttaatga acggccgttg 320<210> 191<211> 320<212> RNA<213> Mycobacterium chelonae<400> 191acagcgaguc ucgacuuaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60auugcaacca auuaagcgcc gauucucauc agcgcgacua cgcacucgcu gccuaagcga 120cugcgugucu gucagaccgg gagcgcccuc agcccggacc cuggcaucag cuagagggac 180aaacuacggg uucggucgcg ggacccguag ggacaucaaa cagcgacugg gaucgucauc 240ucggcuuguu cgcgggaccg agagauccaa guagaggcau agcgaacugc gcacggagaa 300gccuuaauga acggccguug 320<210> 192<211> 320<212> DNA<213> Mycobacterium szulgai<400> 192acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gagaacactc agcgcgactt cgctctcgct gcctaagcga 120cagcaagtcc gtcagaccgg gaaagccctc gacccggacc ctggcgtcat ctagagggat 180ccaccggtga gttcggtcgc gggactcatc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtt cgcgtgacca ggagatccga gtagagacat agcgaactgc gcacggagaa 300gccttgaggg aatgccgtag 320<210> 193<211> 320<212> RNA<213> Mycobacterium szulgai<400> 193acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gagaacacuc agcgcgacuu cgcucucgcu gccuaagcga 120cagcaagucc gucagaccgg gaaagcccuc gacccggacc cuggcgucau cuagagggau 180ccaccgguga guucggucgc gggacucauc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguu cgcgugacca ggagauccga guagagacau agcgaacugc gcacggagaa 300gccuugaggg aaugccguag 320<210> 194<211> 320<212> DNA<213> Mycobacterium malmoense<400> 194acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca tataagcgcc gtttcaacac agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcagaccgg gaacgccctc gacccggagc ctggcgtcag ctggagggat 180ccaccggtga gtccggtcgc gggactcatc gggacataca cagcgactgg gatcgtcatc 240ctggctggtt cgcgtgaccg ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgtag 320<210> 195<211> 320<212> RNA<213> Mycobacterium malmoense<400> 195acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca uauaagcgcc guuucaacac agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucagaccgg gaacgcccuc gacccggagc cuggcgucag cuggagggau 180ccaccgguga guccggucgc gggacucauc gggacauaca cagcgacugg gaucgucauc 240cuggcugguu cgcgugaccg ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccguag 320<210> 196<211> 321<212> DNA<213> Mycobacterium flavescens<400> 196acttcgagcg tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gattccaatc agcgcgacta cgcactcgct gcctaagcga 120ctgcgtgtct gtcagcccgg gagagccctc gacccggtgt ctggcatcag ctagagggat 180aaaccggtgg gtccggtcgc gggactcatc gggacatcaa acagcgactg ggatcgtcat 240cctgacttgt tcgcgtgatc aggagatccg agtagagaca tagcgaactg cgcacggaga 300agccttgagg gaacgccgta g 321<210> 197<211> 321<212> RNA<213> Mycobacterium flavescens<400> 197acuucgagcg ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gauuccaauc agcgcgacua cgcacucgcu gccuaagcga 120cugcgugucu gucagcccgg gagagcccuc gacccggugu cuggcaucag cuagagggau 180aaaccggugg guccggucgc gggacucauc gggacaucaa acagcgacug ggaucgucau 240ccugacuugu ucgcgugauc aggagauccg aguagagaca uagcgaacug cgcacggaga 300agccuugagg gaacgccgua g 321<210> 198<211> 320<212> DNA<213> Mycobacterium marinum<400> 198acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gatgcaacta gataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcggaccgg gaacgccctc gccccggacc ccggcatcag ctagagggat 180caaccgatga gttcggtcgc ggggctcatc gggacatcaa cagcgactgg gatcgtcatc 240ctggctagtt cgcgtgacca ggagatccga gcagagacct agcggactgc gcacggagaa 300gccttgaggg aatgccgtag 320<210> 199<211> 320<212> RNA<213> Mycobacterium marinum<400> 199acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60gaugcaacua gauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucggaccgg gaacgcccuc gccccggacc ccggcaucag cuagagggau 180caaccgauga guucggucgc ggggcucauc gggacaucaa cagcgacugg gaucgucauc 240cuggcuaguu cgcgugacca ggagauccga gcagagaccu agcggacugc gcacggagaa 300gccuugaggg aaugccguag 320<210> 200<211> 319<212> DNA<213> Mycobacterium microti<400> 200acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcgacca aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cggctagtct gtcagaccgg gaacgccctc ggcccggacc ctggcatcag ctagagggat 180ccaccgatga gtccggtcgc gggactcctc gggacagcca cagcgactgg gatcgtcatc 240tcggctagtt cgcgtgaccg ggagatccga gcagaggcat agcgaactgc gcacggagaa 300gccttgaggg aatgccgta 319<210> 201<211> 319<212> RNA<213> Mycobacterium microti<400> 201acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcgacca aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cggcuagucu gucagaccgg gaacgcccuc ggcccggacc cuggcaucag cuagagggau 180ccaccgauga guccggucgc gggacuccuc gggacagcca cagcgacugg gaucgucauc 240ucggcuaguu cgcgugaccg ggagauccga gcagaggcau agcgaacugc gcacggagaa 300gccuugaggg aaugccgua 319<210> 202<211> 321<212> DNA<213> Mycobacterium smegmatis<400> 202acttcgagca tcgaatccag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gattccaatc agcgcgacta cgccctcgct gcctaagcga 120cggctggtct gtcagaccgg gagtgccctc ggcccggatc ctggcatcag ctagagggac 180ccacccacgg gttcggtcgc gggacctgtg gggacatcaa acagcgactg ggatcgtcat 240ctcggcttgt tcgtgtgacc gggagatccg agtagagaca tagcgaactg cgcacggaga 300agcctcgagg acatgccgta g 321<210> 203<211> 321<212> RNA<213> Mycobacterium smegmatis<400> 203acuucgagca ucgaauccag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gauuccaauc agcgcgacua cgcccucgcu gccuaagcga 120cggcuggucu gucagaccgg gagugcccuc ggcccggauc cuggcaucag cuagagggac 180ccacccacgg guucggucgc gggaccugug gggacaucaa acagcgacug ggaucgucau 240cucggcuugu ucgugugacc gggagauccg aguagagaca uagcgaacug cgcacggaga 300agccucgagg acaugccgua g 321<210> 204<211> 320<212> DNA<213> Mycobacterium xenopi<400> 204acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacta aataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcaggccgg gagttccctc gacccggatc ctggcgtcag ctagagggat 180ccaccgatgg gttcggtcgc gggacccatc gggacaccac acagcgactg ggatcgccgt 240cccggctagt tcgcgagacc gggagatccg agtaagggca aagcgaactg cgcacggaga 300agccttgagg gtatgccgta 320<210> 205<211> 320<212> RNA<213> Mycobacterium xenopi<400> 205acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacua aauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucaggccgg gaguucccuc gacccggauc cuggcgucag cuagagggau 180ccaccgaugg guucggucgc gggacccauc gggacaccac acagcgacug ggaucgccgu 240cccggcuagu ucgcgagacc gggagauccg aguaagggca aagcgaacug cgcacggaga 300agccuugagg guaugccgua 320<210> 206<211> 320<212> DNA<213> Mycobacterium intracellulare<400> 206acttcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aaccgaccac cgtaagcgtc 60gttgcaaaca gataagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcagaccgg gaacgccctc gacccggagc ctggcgtcag ctagagggat 180ccaccgatga gtccggtcgc gggacttatc gggacaccaa cagcgactgg gatcgtcatc 240tcggcttgtt cgcgtgaccg ggagatccga gtagaggcat agcgaactgc gcacggagaa 300gtcttgaggg aatgccgtag 320<210> 207<211> 320<212> RNA<213> Mycobacterium intracellulare<400> 207acuucgcgca ucgaaucaag ggaagcgugc cggugcaggc aaccgaccac cguaagcguc 60guugcaaaca gauaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucagaccgg gaacgcccuc gacccggagc cuggcgucag cuagagggau 180ccaccgauga guccggucgc gggacuuauc gggacaccaa cagcgacugg gaucgucauc 240ucggcuuguu cgcgugaccg ggagauccga guagaggcau agcgaacugc gcacggagaa 300gucuugaggg aaugccguag 320<210> 208<211> 320<212> DNA<213> Mycobacterium scrofulaceum<400> 208acatcgcgca tcgaatcaag ggaagcgtgc cggtgcaggc aagagaccac cgtaagcgtc 60gttgcaacca attaagcgcc gattcacatc agcgcgacta cgctctcgct gcctaagcga 120cagctagtcc gtcagaccgg gaaagccctc gacccggagc ctggcgtcag ctagagggat 180caaccgatga gttcggtcgc gggactcatc gggacaccaa cagcgactgg gatcgtcatc 240ctggctagtc cgcgtgacca ggagatccga gcagaggcat agcggactgc gcacggagaa 300gtcttgaggg aatgccgttg 320<210> 209<211> 320<212> RNA<213> Mycobacterium scrofulaceum<400> 209acaucgcgca ucgaaucaag ggaagcgugc cggugcaggc aagagaccac cguaagcguc 60guugcaacca auuaagcgcc gauucacauc agcgcgacua cgcucucgcu gccuaagcga 120cagcuagucc gucagaccgg gaaagcccuc gacccggagc cuggcgucag cuagagggau 180caaccgauga guucggucgc gggacucauc gggacaccaa cagcgacugg gaucgucauc 240cuggcuaguc cgcgugacca ggagauccga gcagaggcau agcggacugc gcacggagaa 300gucuugaggg aaugccguug 320<210> 210<211> 326<212> DNA<213> Nocardia asteroides<400> 210actgtgtgcg ccgaggtagg ggaagcgtgt cggtgcaggc tggagaccac cgttaagcgt 60cgcggcaacc aattaagcgc cgattccaat cagcgcgact acgccctcgc tgcctgatca 120gcgacggcta gctgtcggcc cgggttgtgt tcccgaaccc ggatgccggc atcatctcag 180ggaactcacc gtgttcgccg gtcgcggacg gacacgggac agcaaacagc gactgggatc 240gtcatctcgg cttgttcgcg tgaccgggag atccaagtag agacatagcg gactgcacac 300ggagaagccc tactgactcg acacag 326<210> 211<211> 325<212> RNA<213> Nocardia asteroides<400> 211acugugugcg ccgagguagg ggaagcgugu cggugcaggc uggagaccac cguuaagcgu 60cgcggcaacc aauuaagcgc cgauuccaau cagcgcgacu acgcccucgc ugccugauca 120gcgacggcua gcugucggcc cggguugugu ucccgaaccc ggaugccggc aucaucucag 180ggaacucacc guguucgccg gucgcggacg gacacgggac agcaaacagc gacugggauc 240gucaucucgg cuuguucgcg ugaccgggag auccaaguag agacauagcg gcugcacacg 300gagaagcccu acugacucga cacag 325<210> 212<211> 314<212> DNA<213> Salmonella enteritidis<400> 212acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca 60aaaaaatagt cgcaaacgac gaaacctacg ctttagcagc ttaataacct gcttagagcc 120ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat 180cgcgtggatg ccctgcctgg ggttgaagcg ttaaaacgaa tcaggctagt ctggtagtgg 240cgtgtccgtc cgcaggtgcc aggcgaatgt aaagactgac taagcatgta gtaccgagga 300tgtaggaatt tcgg 314<210> 213<211> 314<212> RNA<213> Salmonella enteritidis<400> 213acgggauuug cgaaacccaa ggugcaugcc gaggggcggu uggccucgua aaaagccgca 60aaaaaauagu cgcaaacgac gaaaccuacg cuuuagcagc uuaauaaccu gcuuagagcc 120cucucucccu agccuccgcu cuuaggacgg ggaucaagag aggucaaacc caaaagagau 180cgcguggaug cccugccugg gguugaagcg uuaaaacgaa ucaggcuagu cugguagugg 240cguguccguc cgcaggugcc aggcgaaugu aaagacugac uaagcaugua guaccgagga 300uguaggaauu ucgg 314<210> 214<211> 313<212> DNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 214acaggggtcc cccgagctta ttaagcgtgt cggagggttg gctccgtcat caacacattt 60cggttaaata taactgacaa atcaaacaat aatttcgcag tagctgcgta atagccactg 120catcgcctaa cagcatctcc tacgtgctgt taacgcgatt caaccctagt aggatatgct 180aaacactgcc gcttgaagtc tgtttagatg aaatataatc aagctagtat catgttggtt 240gtttattgct tagcatgatg cgaaaattat caataaacta cacacgtaga aagatttgta 300tcaggacctc tgg313<210> 215<211> 313<212> RNA<213> Staphylococcus epidermidis (NCTC 11047)<400> 215acaggggucc cccgagcuua uuaagcgugu cggaggguug gcuccgucau caacacauuu 60cgguuaaaua uaacugacaa aucaaacaau aauuucgcag uagcugcgua auagccacug 120caucgccuaa cagcaucucc uacgugcugu uaacgcgauu caacccuagu aggauaugcu 180aaacacugcc gcuugaaguc uguuuagaug aaauauaauc aagcuaguau cauguugguu 240guuuauugcu uagcaugaug cgaaaauuau caauaaacua cacacguaga aagauuugua 300ucaggaccuc ugg313<210> 216<211> 302<212> DNA<213> Streptococcus agalactiae (NCTC 8181)<400> 216acaggcatta tgaggtatat tttgcgactc atcggcagat gtaaaatgcc agttaaatat 60aactgcaaaa aatacaaatt cttacgcatt agctgcctaa aaaacagcct gcgtgatctt 120cacaagattg tttgcgtttt gctagaaggt cttatttatc agcaaactac gtttggctac 180tgtctagtta gttaaaaaga gatttataga ctcgctatgt gagggcttga gttatgtgtc 240atcacctagt taaatcaata cataacctat agttgtagac aaatatatta gcagatgttt 300gg302<210> 217<211> 302<212> RNA<213> Streptococcus agalactiae (NCTC 8181)<400> 217acaggcauua ugagguauau uuugcgacuc aucggcagau guaaaaugcc aguuaaauau 60aacugcaaaa aauacaaauu cuuacgcauu agcugccuaa aaaacagccu gcgugaucuu 120cacaagauug uuugcguuuu gcuagaaggu cuuauuuauc agcaaacuac guuuggcuac 180ugucuaguua guuaaaaaga gauuuauaga cucgcuaugu gagggcuuga guuauguguc 240aucaccuagu uaaaucaaua cauaaccuau aguuguagac aaauauauua gcagauguuu 300gg302<210> 218<211> 21<212> DNA<213> 人工序列<220><223> 人工序列的描述Salmonella屬特異性探針<400> 218cgaatcaggc tagtctggta g 21<210> 219<211> 23<212> DNA<213> 人工序列<220><223> 人工序列的描述檢測肺結(jié)核復(fù)合體的寡核苷酸探針<400> 219actcctcggg acarccacag cga 23<210> 220<211> 20<212> DNA<213> 人工序列<220><223> 人工序列的描述檢測M.avium和M.Paratuberculosis序列的寡核苷酸探針<400> 220gttgcaaata gataagcgcc 20<210> 221<211> 20<212> DNA<213> 人工序列<220><223> 人工序列的描述檢測M.Avium和M.paratuberculosis序列的寡核苷酸探針<400> 221tccgtcagcc cgggaacgcc 20<210> 222<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述在對Listeria菌細胞進行熱殺滅處理后在tmRNA完整性的測定中所用寡核苷酸探針<400> 222ttttgttttt ctttgcca 18<210> 223<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述在對Escherichia coli細胞進行熱殺滅處理后在tmRNA完整性的測定中所用的寡核苷酸探針<400> 223agttttcgtc gtttgcga 18<210> 224<211> 20<212> DNA<213> 人工序列<220><223> 人工序列的描述用于擴增所有分支桿菌序列的簡并寡核苷酸引物<400> 224caggcaashg accaccgtaa 20<210> 225<211> 23<212> DNA<213> 人工序列<220><223> 人工序列的描述用于擴增所有分支桿菌序列的簡并寡核苷酸引物<400> 225ggatctccyg rtcwcrcgra cwa 23<210> 226<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述用于擴增M.avium和M.paratuberculosis序列的寡核苷酸引物<400> 226tgccggtgca ggcaactg 18<210> 227<211> 18<212> DNA<213> 人工序列<220><223> 人工序列的描述用于擴增M.avium和M.Paratuberculosis序列的寡核苷酸引物<400> 227cacgcgaaca agccagga 18<210> 228<211> 23<212> DNA<213> 人工序列<220><223> 人工序列的描述用于檢測Listeria ssrA基因序列的寡核苷酸探針<400> 228cattaaactt tagcaaggaa gtg 2權(quán)利要求
1.ssrA基因或其片段的用途，其作為用于原核生物或真核生物的核酸探針檢驗中的靶點區(qū)。
2.根據(jù)權(quán)利要求1的用途，其中將ssrA基因分子中與從該DNA分子的5’末端起的高同源區(qū)相對應(yīng)的片段作為通用靶點序列。
3.根據(jù)權(quán)利要求1的用途，其中將ssrA基因分子中與從該DNA分子的3’末端起的高同源區(qū)相對應(yīng)的片段作為通用靶點序列。
4.根據(jù)權(quán)利要求1的用途，其中將ssrA基因分子中與低同源區(qū)相對應(yīng)的片段作為核酸探針檢驗中的靶點區(qū)用于區(qū)分不同物種。
5.根據(jù)權(quán)利要求1的用途，其中將ssrA基因分子中與低同源區(qū)相對應(yīng)的片段作為靶點區(qū)用于產(chǎn)生屬特異性探針。
6.tmRNA即ssrA基因的RNA轉(zhuǎn)錄序列或其片段的用途，其作為用于原核生物或真核生物的核酸探針檢驗中的靶點區(qū)。
7.根據(jù)權(quán)利要求6的用途，其中將tmRNA分子中與從tmRNA分子的5’末端起的高同源區(qū)相對應(yīng)的片段作為通用靶點序列。
8.根據(jù)權(quán)利要求6的用途，其中將tmRNA分子中與從tmRNA分子的3’末端起的高同源區(qū)相對應(yīng)的片段作為通用靶點序列。
9.根據(jù)權(quán)利要求6的用途，其中將tmRNA分子中與低同源區(qū)相對應(yīng)的片段作為核酸探針檢驗中的靶點區(qū)用于區(qū)分不同物種。
10.根據(jù)權(quán)利要求6的用途，其中將tmRNA分子中與低同源區(qū)相對應(yīng)的片段作為靶點區(qū)用于產(chǎn)生屬特異性探針。
11.根據(jù)前面的任一權(quán)利要求的用途，其中所說的ssrA基因片段或tmRNA片段被用作為在擴增中所用的引物的基礎(chǔ)。
12.根據(jù)權(quán)利要求11的用途，其中擴增產(chǎn)物被用來作為核酸探針檢驗的靶點區(qū)。
13.根據(jù)權(quán)利要求6-12中的任何一項所述的用途，其中tmRNA分子的cDNA轉(zhuǎn)錄序列被用作為核酸雜交檢驗中的探針。
14.根據(jù)前面的任一權(quán)利要求所述的用途，其中所述的檢驗在體外完成。
15.根據(jù)權(quán)利要求1-13中的任何一項所述的用途，其中所述的檢驗在原位完成。
16.根據(jù)前面任一權(quán)利要求所述的用途，其中的靶點區(qū)被作為用于區(qū)分活的和死的原核生物或真核生物的檢驗的基礎(chǔ)。
17.根據(jù)前面任一權(quán)利要求所述的用途，其中的靶點區(qū)被用于多探針形式以大范圍地檢測和/或鑒定原核生物或真核生物。
18.根據(jù)權(quán)利要求17的用途，其中的ssrA基因探針或tmRNA轉(zhuǎn)錄序列探針被連接到微陣列基因芯片系統(tǒng)上，用于大范圍地高通量檢測和鑒定原核生物或真核生物。
19.根據(jù)前面任一權(quán)利要求所述的用途，其中的靶點區(qū)被用作為用于檢測目標樣品中原核生物或真核生物的檢驗中的探針。
20.根據(jù)權(quán)利要求1-13中的任何一項所述的用途，其中的ssrA基因或tmRNA轉(zhuǎn)錄序列的片段被用于得到原核生物或真核生物的DNA圖譜的檢驗中，從而區(qū)分同一物種的不同株系。
21.根據(jù)權(quán)利要求1-13中的任何一項所述的用途，其中的ssrA基因、tmRNA轉(zhuǎn)錄序列或與其互補的DNA序列或其片段被用來設(shè)計用于治療目的來對抗感染性的原核生物或真核生物的試劑。
22.根據(jù)權(quán)利要求1-15中的任何一項所述的用途，其中的靶點區(qū)被用于監(jiān)測對抗感染性媒介的藥物的治療效率。
23.根據(jù)權(quán)利要求1-15中的任何一項所述的用途，其中的靶點區(qū)被用于監(jiān)測胃腸道中促進健康的微生物的存活率和水平。
24.根據(jù)權(quán)利要求1-15中的任何一項所述的用途，其中所述檢驗被用于原核生物或真核生物的定量分析。
25.根據(jù)權(quán)利要求1-5和11-15中的任何一項所述的用途，其中ssrA基因序列的數(shù)據(jù)庫被用來鑒定原核生物或真核生物。
26.ssrA序列，其選自SEQ ID NO47、49、51、53、55、57、59、61、65、67、71、99、159、161、163、165、167、176、178、180、182、184、186、188、190、192、194、196、198、200、202、204、206、208、210、212、214和216。
27.tmRNA序列，其選自SEQ ID NO48、50、52、54、56、58、60、62、66、68、72、98、160、162、164、168、177、179、181、183、185、187、189、191、193、195、197、199、201、203、205、207、209、211、213、215和217。
28.寡核苷酸探針，其選自SEQ ID NO169、174、175、218-223和228。
29.寡核苷酸引物，其選自SEQ ID NO170-173和224-227。
全文摘要
描述了能夠作為核酸探針檢驗的靶點區(qū)來檢測并鑒別原核生物和/或真核生物的ssrA基因或tmRNA即ssrA基因的RNA轉(zhuǎn)錄序列,或其片段。不同生物的tmRNA序列的核酸排列對比可用于鑒別序列內(nèi)的同源區(qū)和非同源區(qū),這些區(qū)域又可以用來設(shè)計屬特異性和種特異性的寡聚核苷酸探針。這些新鑒定的同源和非同源區(qū)提供了在分子水平鑒別和檢測生物體的基礎(chǔ)。以此方式鑒別出的這種寡核苷酸探針可用于檢測樣品中的tmRNA,由此能夠指示不同樣品類型中存在的非病毒生物的生存能力。
文檔編號A61P31/04GK1350592SQ00807503
公開日2002年5月22日 申請日期2000年5月15日 優(yōu)先權(quán)日1999年5月14日
發(fā)明者托馬斯·杰勒德·巴里, 特倫斯·詹姆斯·史密斯 申請人:愛爾蘭公司(愛爾蘭生物研究), 愛爾蘭戈爾韋國立大學(xué)