專利名稱：表皮葡萄球菌調(diào)節(jié)信號多肽及編碼的dna的制作方法
技術(shù)領(lǐng)域：
本發(fā)明屬生物技術(shù)領(lǐng)域，具體涉及作為表皮葡萄球菌信號傳遞系統(tǒng)關(guān)鍵新蛋白組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白，以及利用該類蛋白尋找抑菌藥物的方法。更具體地，本發(fā)明涉及編碼該類多肽的DNA；制備該類多肽的方法，包括基因克隆、基因表達(dá)、蛋白純化和功能測定；制備該類多肽的抗體；以及利用該類多肽篩選抑菌活性化合物。
背景技術(shù)：
葡萄球菌與醫(yī)院內(nèi)化膿性感染相關(guān)，尤以金黃色葡萄球菌和表皮葡萄球菌最為密切，表皮葡萄球菌所致感染與一些高分子醫(yī)療器材在體內(nèi)的留置有關(guān)，如靜脈導(dǎo)管、起搏器、腦脊液引流裝置、人工器官等。隨著上述醫(yī)療材料的使用日益頻繁，此類院內(nèi)感染問題日益嚴(yán)峻，且易發(fā)展為慢性感染，對抗生素的抗性較高。
已知在各種原核生物中普遍存在雙組分調(diào)控系統(tǒng)，為原核細(xì)胞所特有的信號系統(tǒng)，參與調(diào)控生物生長、毒力等多種生物學(xué)功能。通常由一對相應(yīng)的蛋白組成，稱為組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白。組氨酸蛋白激酶為跨膜蛋白，當(dāng)外界信號作用于組氨酸蛋白激酶的膜外區(qū)域，產(chǎn)生信號，使該蛋白發(fā)生自身磷酸化。隨后，磷酸化的組氨酸蛋白激酶作用于相應(yīng)的反應(yīng)調(diào)節(jié)蛋白發(fā)揮調(diào)控作用。不同的雙組分蛋白，調(diào)控細(xì)菌的不同生物學(xué)活性。然而，由于研究手段的局限性，對表皮葡萄球菌雙組分調(diào)控系統(tǒng)及對生物學(xué)功能的調(diào)控缺乏系統(tǒng)地了解和實(shí)際應(yīng)用。
發(fā)明內(nèi)容
本發(fā)明的目的是提供一組表皮葡萄球菌信號傳遞系統(tǒng)的新蛋白，包括組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白。本發(fā)明的另一目的是提供制備該類多肽的方法和編碼該類多肽的DNA，及其制備該類多肽的抗體，本發(fā)明的進(jìn)一步目的是利用該類多肽篩選藥物化合物。
本發(fā)明通過對表葡菌ATCC12228株的全基因組測序和序列分析，采用序列同源性比較和功能域分析等生物信息學(xué)方法確定了16對表皮葡萄球菌的雙組分調(diào)控信號蛋白(包括組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白)，并制備了相應(yīng)的蛋白，經(jīng)對上述蛋白功能域空間結(jié)構(gòu)的模建和實(shí)驗(yàn)證實(shí)該類蛋白存在保守性結(jié)構(gòu)和活性位點(diǎn)，可作為篩選藥物的“靶點(diǎn)”。
本發(fā)明表皮葡萄球菌組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白中包括16對雙組分調(diào)控蛋白，其中16個組氨酸蛋白激酶均具有結(jié)合ATP的激酶活性功能域(HATPase_c功能域)，分別具有SEQ ID No.1-16的序列結(jié)構(gòu)。其中16個反應(yīng)調(diào)節(jié)蛋白均存在接受磷酸基團(tuán)的功能域(REC功能域)，分別具有SEQ ID No.17-32的序列結(jié)構(gòu)。
本發(fā)明采用PMODELIHG軟件對上述相對保守的功能域片段進(jìn)行同源模建，結(jié)果顯示組氨酸蛋白激酶HATPase_c功能域的結(jié)構(gòu)特征是在AMP-PNP的結(jié)合部位形成袋狀結(jié)構(gòu)，且在其周圍有保守的基序圍繞，所述AMP-PNP是一種ATP類似物；反應(yīng)調(diào)節(jié)蛋白的REC功能域模擬結(jié)構(gòu)由5個α螺旋和5個β折疊組成，其中存在三個天冬氨酸殘基構(gòu)成接受磷酸基團(tuán)的活性位點(diǎn)，且接受磷酸基團(tuán)的過程需要二價陽離子(Mg2+或Mn2+)的輔助，所述天冬氨酸殘基是D7、D8、D51。
本發(fā)明所述的表皮葡萄球菌組氨酸蛋白激酶具有SEQ ID No.1-16的氨基酸序列結(jié)構(gòu)，由SEQ ID No.33-48的DNA核苷酸序列編碼；所述的反應(yīng)調(diào)節(jié)蛋白具有SEQ ID No.17-32的氨基酸序列結(jié)構(gòu)，由SEQ ID No.49-64的DNA核苷酸序列編碼。
本發(fā)明涉及由SEQ ID No.1-32構(gòu)成的蛋白的氨基酸序列的同源序列及片斷序列，和SEQ ID No.33-64編碼相關(guān)同源序列和片斷有關(guān)多肽的DNA核苷酸序列。所述同源DNA序列是指編碼SEQ ID No.33-64所示核苷酸序列或片斷，或DNA片斷長度在60個連續(xù)核苷酸區(qū)域內(nèi)，一般至少70％同源于SEQ ID 1-32，可與之選擇性雜交的片斷核苷酸序列。所述SEQ ID 1-32的多肽同源序列至少在30個的連續(xù)氨基酸序列區(qū)域，至少60％以上的同源。
本發(fā)明的多肽包括組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白氨基酸序列的功能域部分序列，如具有生物學(xué)活性的氨基酸基本序列的多肽片斷；或其中部分氨基酸被性質(zhì)相似的氨基酸取代的多肽；或其氨基酸的一部分中加入或插入其他氨基酸的多肽，以及DNA的核苷酸序列改變，但不影響氨基酸序列的多肽，如同種氨基酸使用不同密碼子。
本發(fā)明提供了制備表皮葡萄球菌組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白(SEQ IDNo.1-32)方法，包括制備僅含生物學(xué)活性功能區(qū)的編碼序列的DNA片斷，克隆至適合的表達(dá)載體，轉(zhuǎn)染宿主菌，在適于的條件下培養(yǎng)該宿主細(xì)胞，以及從培養(yǎng)物中回收和純化所需蛋白。
上述技術(shù)方案中所有基本分子生物學(xué)操作均參照《分子克隆實(shí)驗(yàn)指南》。
本發(fā)明將編碼相應(yīng)蛋白的DNA序列與表達(dá)載體重組，形成重組表達(dá)質(zhì)粒。本發(fā)明不限于特定的表達(dá)質(zhì)粒。在一優(yōu)選實(shí)施方案中，本發(fā)明使用原核表達(dá)載體，例如pet28a等。
上述重組表達(dá)載體可按常規(guī)方法導(dǎo)入適宜宿主細(xì)菌。本發(fā)明不局限于任何特定的宿主細(xì)胞，只要它能夠表達(dá)所述重組表達(dá)載體。在一優(yōu)選實(shí)施方案中，本發(fā)明使用大腸桿菌JM109等。
本發(fā)明的多肽包括磷酸化反應(yīng)產(chǎn)物。
本發(fā)明還提供抗本發(fā)明多肽的多克隆抗體或單克隆抗體，使用本發(fā)明的多肽或其片斷作為免疫原，按常規(guī)方法制備特異性抗體?？捎糜诩?xì)菌細(xì)胞內(nèi)的多肽表達(dá)量，也可用于研究所述多肽與細(xì)菌致病的關(guān)系，或用于阻斷細(xì)菌細(xì)胞之間的信號轉(zhuǎn)遞。
可以將本發(fā)明DNA的反義核酸的DNA插入合適的載體中，產(chǎn)生反義RNA；也可用合成方法產(chǎn)生反義RNA。反義RNA可用于控制細(xì)菌細(xì)胞內(nèi)多肽水平。
本發(fā)明多肽功能域結(jié)構(gòu)可用于篩選活性化合物，能阻斷組氨酸蛋白激酶或反應(yīng)調(diào)節(jié)蛋白質(zhì)間的磷酸基團(tuán)轉(zhuǎn)移和蛋白質(zhì)的磷酸化，抑制細(xì)菌細(xì)胞內(nèi)或細(xì)胞之間的信號傳遞，從而抑制葡萄球菌的生長。
本發(fā)明在表皮葡萄球菌ATCC12228株中確定了16對完整的雙組分調(diào)控系統(tǒng)的基因核苷酸序列及其功能。經(jīng)實(shí)驗(yàn)證實(shí)了組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白的磷酸化或兩者分子之間的磷酸基團(tuán)轉(zhuǎn)移可調(diào)控葡萄球菌的生物學(xué)活性，抑制組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白的磷酸化或磷酸基團(tuán)的轉(zhuǎn)移可抑制細(xì)菌的生長。對其保守性功能域的空間結(jié)構(gòu)模建確定了存在保守結(jié)構(gòu)和活性位點(diǎn)可作為篩選活性化合物的藥物“靶點(diǎn)”。
圖1是表皮葡萄球菌雙組分調(diào)控蛋白保守功能域空間結(jié)構(gòu)同源模建圖。其中，1a是組氨酸蛋白激酶HATPase_c功能域模擬結(jié)構(gòu)，1b是反應(yīng)調(diào)節(jié)蛋白REC功能域模擬結(jié)構(gòu)。
具體實(shí)施例方式
實(shí)施例1 確定16對表皮葡萄球菌雙組分調(diào)控系統(tǒng)運(yùn)用序列同源性比較，功能域分析等生物信息學(xué)方法，在表皮葡萄球菌ATCC12228株全基因組(GeneBank收錄號AE015929)中確定了16對雙組分調(diào)控基因。表1列出了上述雙組分調(diào)控基因的基本情況，包括基因長度等，以及氨基酸序列同源性比較結(jié)果(blastp)。
表1
通過與SMART氨基酸序列及功能域數(shù)據(jù)庫進(jìn)行同源性比較，對16對雙組分調(diào)控基因中存在的保守性功能域進(jìn)行分析，結(jié)果表明16個組氨酸蛋白激酶中均存在結(jié)合ATP的激酶活性功能域(HATPase_c功能域)，16個反應(yīng)調(diào)節(jié)蛋白(SEQID No.17-32)均存在接受磷酸基團(tuán)的功能域(REC功能域)，表2是表皮葡萄球菌雙組分調(diào)控基因功能域分析結(jié)果。
表2
其中功能域名稱均來源于SMART氨基酸序列數(shù)據(jù)庫。
實(shí)施例2 對HATPase_c功能域和REC功能域進(jìn)行空間結(jié)構(gòu)模建根據(jù)上述確定的HATPase_c功能域和REC功能域在雙組分調(diào)控系統(tǒng)中的相對保守性，運(yùn)用PMODELING軟件對兩個功能域進(jìn)行空間結(jié)構(gòu)的同源模建。結(jié)果顯示HATPase_c功能域模擬結(jié)構(gòu)，與已報道的大腸桿菌組氨酸蛋白激酶基因EnvZ的HATPase_c功能域的和磁共振(NMR)結(jié)構(gòu)非常相似(Ncbi mmdbId11091)，即HATPase_c功能域主要結(jié)構(gòu)由4個β折疊和3個α螺旋組成，尤其是在AMP-PNP(一種ATP類似物)的結(jié)合部位形成一個袋狀結(jié)構(gòu)，且其周圍有一些保守的基序(G1 box、G2 box、F box)圍繞，見圖1a；而REC功能域模擬結(jié)構(gòu)與大腸桿菌的反應(yīng)調(diào)節(jié)蛋白基因CheY的REC功能域的和磁共振(NMR)結(jié)構(gòu)也非常相似(NcbimmdbId3000)，由5個α螺旋和5個β折疊組成，其中存在三個天冬氨酸殘基(D7、D8、D51)構(gòu)成接受磷酸基團(tuán)的活性位點(diǎn)，且該過程需要二價陽離子(Mg2+或Mn2+)的輔助，見圖1b。因此，這兩個結(jié)構(gòu)均存在藥物設(shè)計、篩選的“靶標(biāo)”，以它們?yōu)槟０澹Y選各類小分子化合物庫，能找到一些在空間結(jié)構(gòu)上封閉該活性結(jié)構(gòu)或活性位點(diǎn)的小分子化合物，從而阻斷這些雙組分調(diào)控系統(tǒng)，其作用原理是阻斷雙組分調(diào)控系統(tǒng)信號傳導(dǎo)途徑的一個或多個環(huán)節(jié)，包括組氨酸蛋白激酶的自身磷酸化、將磷酸基團(tuán)從蛋白激酶轉(zhuǎn)移到調(diào)節(jié)蛋白的轉(zhuǎn)磷酸基過程以及反應(yīng)調(diào)節(jié)蛋白的磷酸化等。
實(shí)施例3 16對雙組分調(diào)控基因相關(guān)功能的分析將16對雙組分調(diào)控基因與已報道的其他革蘭陽性菌中有明確功能的雙組分基因進(jìn)行了序列同源性比較，結(jié)果發(fā)現(xiàn)有8對雙組分調(diào)控基因與已報道的其他革蘭陽性菌中雙組分基因有較高的氨基酸序列同源性(同源性大于30％)，表明所述雙組分調(diào)控系統(tǒng)在同為革蘭陽性菌的表皮葡萄球菌中也可能發(fā)揮著重要的作<p>表2-(4周時的結(jié)果)
將上述重組質(zhì)粒1175-pet28a、1176-pet28a、1175d-pet28a按常規(guī)方法在IPTG誘導(dǎo)下表達(dá)，用SDS-PAGE電泳對目的蛋白進(jìn)行鑒定，結(jié)果顯示三種重組質(zhì)粒分別表達(dá)70kd、30kd及30kd左右的蛋白，用申能博彩公司提供的3s NTAHis-Tag Resin層析柱進(jìn)行蛋白提純。
實(shí)施例5 其余1 5對雙組分基因(除外se1175/se1176)原核表達(dá)質(zhì)粒的構(gòu)建以表葡菌ATCC12228株全基因組DNA為模板，設(shè)計相應(yīng)引物經(jīng)PCR獲得。
se0019引物設(shè)計正義鏈5’CGGGGA TCCATG AAG TGG CTT AAA CAA CTA 3’反義鏈5’GGC TTA TTC ATC CCA ATC ACC GTC 3’se0121引物設(shè)計正義鏈5’CGGGGA TCCATG GCT AGA GTA GAA AGT TTA 3’反義鏈5’GGC TTA GAC AGG AAT ATT GAA ACT 3’se0166引物設(shè)計正義鏈5’CGGGGA TCCATG AAG ACC TAT AAG CCG TAC 3’反義鏈5’GGC CTA GAC AAG TGG TAT TTG GTA 3’se0428引物設(shè)計正義鏈5’CGG GGA TCC ATG AAT AAT TTT CGA TGG TTT 3’反義鏈5’GGC TTA AAA TGA CAA TCT TGT CAC 3’se0478引物設(shè)計正義鏈5’CGGGGA TCCATG TGG TTT AAT GGT CAC ATG 3’反義鏈5’GGC TTA CTT TTT AAA TTG AAA TTT 3’se1000引物設(shè)計正義鏈5’CGGGGA TCCATG GAA TCA AGA AGT TGG GGA 3’反義鏈5’GGC TCA AAA GAT TGG TAT CTT CAA 3’se1099引物設(shè)計正義鏈5’CGGGGA TCCATG ATT AAG CGC CAA AAA TTA 3’反義鏈5’GGC TTA GTT TAG TAC TGG AAA ACT 3’se1368引物設(shè)計正義鏈5’CGGGGA TCCATG TTG AAG TTC CAT TTG CGT 3’反義鏈5’GGC TTA ATT ATC ATT AAA TAG AAC 3’
se1530引物設(shè)計正義鏈5’CGGGGA TCCATG GAG AAA CCA ACT AGA CTT 3’反義鏈5’GGC TTA TGC ATA AGG ATG CTC CTT 3’se1570引物設(shè)計正義鏈5’CGG GGA TCC ATG AAT CAC TAC ATA AGA GCT 3’反義鏈5’GGC TTA ATC GCC ACT TGA ATT CTC 3’se1637引物設(shè)計正義鏈5’CGGGGA TCCATG GAT GAT ATT AAT CTA TTT 3’反義鏈5’GGC TTA TGA TTC CTT ATT ATT TAT 3’se1942引物設(shè)計正義鏈5’CGGGGA TCCATG TTT AAA ACA TTA TAT TCG 3’反義鏈5’GGC CTA AAT AAA TAG TTT TAC AAT 3’se1970引物設(shè)計正義鏈5’CGGGGA TCCGTG GTA AAT ATG TTG GAG CAA 3’反義鏈5’GGC TTA AAT CGG TAT ATC TAA TGT 3’se2011引物設(shè)計正義鏈5’CGG GGA TCC ATG AGG ATT GAC GTT ATG CTT 3’反義鏈5’GGC TCA ACG ATT AAC GCT TTC ATT 3’se2194引物設(shè)計正義鏈5’CGGGGA TCCATG AAG TTT GCA TAT ATT CAA 3’反義鏈5’GGC TTA TTT TTC TTT AGT AAA ATG 3’se0018引物設(shè)計正義鏈5’CGGGGA TCCATG GCT AGA AAA GTT GTT GTA 3’反義鏈5’GGC CTA ATC ATG TTG TTG GAG GAA 3’se0120引物設(shè)計正義鏈5’CGGGGA TCCGTG ATG TAT AAC ATA ATG ATT 3’反義鏈5’GGC CTA CTC TAG CCA TTT ATA TCC 3’se0165引物設(shè)計正義鏈5’CGGGGA TCCGTG TTA CCA AAT ACC ACT TGT 3’反義鏈5’GGC CTA TTT TGA TAG CTT ATT ATA 3’
se0427引物設(shè)計正義鏈5’CGGGGA TCCATG CAA ATA TTA TTA GTC GAA 3’反義鏈5’GGC TTA TTC ATG AGC TAA GTA TCC 3’se0479引物設(shè)計正義鏈5’CGG GGA TCCGTG AAG ATA AGC ATG ACC CAC 3’反義鏈5’GGC TTA TCG GCT CCT TTC AAA CTT 3’se1002引物設(shè)計正義鏈5’CGGGGA TCCATG ATA TCG CTT GTT ATA ACT 3’反義鏈5’GGC TTA AAT CCA CCC CTT TTC ATT 3’se1100引物設(shè)計正義鏈5’CGGGGA TCCATG ACA AAT ATT TTA ATT GTA 3’反義鏈5’GGC TCA TCG AAT CAC ATA CCC TAC 3’se1369引物設(shè)計正義鏈5’CGGGGA TCCATG TAT AAA AAG ATT AGG AGG 3’反義鏈5’GGCGTC GACTTA AGC TTT AGG TCT CTC CAG 3’se1529引物設(shè)計正義鏈5’CGGGGA TCCATG CAT AAA GTC ATT CTC GTC 3’反義鏈5’GGC TTA AAT CAA TTT ATT TTC CAT 3’se1569引物設(shè)計正義鏈5’CGGGGA TCCGTG GCG ATT AAA GTT TTA TTT 3’反義鏈5’GGC TTA TTG AAT TAA ATT ATG CTG 3’se1638引物設(shè)計正義鏈5’CGGGGA TCCATG AAA ATT TTT GTT TGT GAA 3’反義鏈5’GGC TTA TAT TTT TTT AAC ATT ACG 3’se1941引物設(shè)計正義鏈5’CGGGGA TCCATG ATT AAT TGC TTA ATC GTA 3’反義鏈5’GGC TTA AAC ATT TTG GTC CAC CCT 3’se1969引物設(shè)計正義鏈5’CGGGGA TCCGTG AAA ATA GTT ATA GCG GAT 3’反義鏈5’GGC TTA AAA TTC TAA TAA TTT TTT 3’
se2012 引物設(shè)計正義鏈5’CGGGGA TCCATG AAA GCG TTA ATC GTT GAT 3’反義鏈5’GGC TTA TTG AAG ACC AAG TTG TTG 3’se2195引物設(shè)計正義鏈5’CGGGGA TCCATG AAG ATT TTC ATT GTA GAA 3’反義鏈5’GGC TCA AAC CCT GTA TCC TAC ATT 3’單劃線代表BamHI內(nèi)切酶位點(diǎn)，雙劃線代表XholI內(nèi)切酶位點(diǎn)，波浪線代表SalI內(nèi)切酶位點(diǎn)，虛線代表EcoRI內(nèi)切酶位點(diǎn)，限制性內(nèi)切酶購自GIBCO公司，大腸桿菌BL21，質(zhì)粒pet28a Invitrogen公司。按常規(guī)分子克隆方法將目的基因或片斷重組入質(zhì)粒pet28a，卡那抗性篩選陽性克隆。
SEQUENCE LISTINGOrganization ApplicantStreet醫(yī)學(xué)院路138號City上海Country中國PostalCode200032PhoneNumber8621-54237524FaxNumber8621-54237524<110>復(fù)旦大學(xué)<120>表皮葡萄球菌調(diào)節(jié)信號多肽及編碼的DNA<160>64<170>PatentIn version 3.2<210>1<211>610<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>1Met Lys Trp Leu Lys Gln Leu Gln Ser Leu His Thr Lys Leu Val Ile1 5 10 15Val Tyr Val Leu Leu Ile Ile Ile Gly Met Gln Ile Ile Gly Leu Tyr20 25 30Phe Thr Asn Ser Leu Glu Lys Glu Leu Leu Asp Asn Phe Lys Lys Asn35 40 45Ile Thr Gln Tyr Ala Lys Gln Leu Asp Val Ash Ile Glu Lys Val Tyr50 55 60Lys Asp Lys Asp Lys Gly Ser Val Asn Ala Gln Lys Asp Ile Gln Asp65 70 75 80Leu Leu Asn Glu Tyr Ala Asn Arg Gln Glu Ile Gly Glu Ile Arg Phe85 90 95Ile Asp Lys Asp Gln Ile Ile Met Ala Thr Thr Lys Gln Ser Asn Arg100 105 110Gly Leu Ile Asn Gln Lys Val Asn Asp Gly Ser Val Gln Lys Ala Leu115 120 125Ser Leu Gly Gln Thr Asn Asp His Met Val Leu Lys Asp Tyr Gly Ser130 135 140Gly Lys Glu Arg Val Trp Val Tyr Asn Ile Pro Val Lys Val Asp Lys145 150 155 160Gln Thr Ile Gly Asp Ile Tyr Ile Glu Ser Lys Ile Asn Asp Val Tyr165 170 175Asn Gln Leu Asn Asn Ile Asn Gln Ile Phe Ile Val Gly Thr Ala Ile180 185 190Ser Leu Phe Ile Thr Val Ile Leu Gly Phe Phe Ile Ala Arg Thr Ile195 200 205
Thr Lys Pro Ile Thr Asp Met Arg Asn Gln Thr Val Glu Met Ser Lys210 215 220Gly Asn Tyr Thr Gln Arg Val Lys Ile Tyr Gly Asn Asp Glu Ile Gly225 230 235 240Glu Leu Ala Leu Ala Phe Asn Asn Leu Ser Lys Arg Val Gln Glu Ala245 250 255Gln Ala Asn Thr Glu Ser Glu Lys Arg Arg Leu Asp Ser Val Ile Thr260 265 270His Met Ser Asp Gly Ile Leu Ala Thr Asp Arg Arg Gly Arg Val Arg275 280 285Ile Ala Asn Asp Met Ala Leu Lys Met Leu Gly Leu Ala Lys Glu Asp290 295 300Val Ile Gly Tyr Tyr Met Leu Gly Val Leu Asn Leu Glu Asn Glu Phe305 310 315 320Ser Leu Glu Glu Ile Gln Glu Asn Ser Asp Ser Phe Leu Leu Asp Ile325 330 335Asn Glu Glu Glu Gly Ile Ile Ala Arg Val Asn Phe Ser Thr Ile Val340 345 350Gln Glu Thr Gly Phe Val Thr Gly Tyr Ile Ala Val Leu His Asp Val355 360 365Thr Glu Gln Gln Gln Val Glu Arg Glu Arg Arg Glu Phe Val Ala Asn370 375 380Val Ser His Glu Leu Arg Thr Pro Leu Thr Ser Met Asn Ser Tyr Ile385 390 395 400Glu Ala Leu Glu Glu Gly Ala Trp Gln Asp Lys Glu Leu Ala Pro Ser405 410 415Phe Leu Ser Val Thr Arg Glu Glu Thr Glu Arg Met Ile Arg Leu Val420 425 430Asn Asp Leu Leu Gln Leu Ser Lys Met Asp Asn Glu Ser Asp Gln Ile435 440 445Thr Lys Glu Ile Ile Asp Phe Asn Met Phe Ile Asn Lys Ile Ile Asn450 455 460Arg His Glu Met Ala Ala Lys Asp Thr Thr Phe Val Arg Glu Ile Pro465 470 475 480Gln Gln Thr Ile Phe Ala Glu Ile Asp Pro Asp Lys Met Thr Gln Val485 490 495Phe Asp Asn Val Ile Thr Asn Ala Met Lys Tyr Ser Arg Gly Glu Lys500 505 510Arg Val Glu Phe His Val Lys Gln Asn Ala Leu Tyr Asn Arg Met Thr515 520 525Ile Arg Ile Lys Asp Asn Gly Ile Gly Ile Pro Ile Asn Lys Val Asp530 535 540Lys Ile Phe Asp Arg Phe Tyr Arg Val Asp Lys Ala Arg Thr Arg Lys545 550 555 560
Met Gly Gly Thr Gly Leu Gly Leu Ala Ile Ser Lys Glu Ile Val Glu565 570 575Ala His Asn Gly Arg Ile Trp Ala Asn Ser Val Glu Gly Gln Gly Thr580 585 590Ser Ile Phe Ile Thr Leu Pro Cys Glu Ile Ile Glu Asp Gly Asp Trp595 600 605Asp Glu610<210>2<211>363<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>2Met Ala Arg Val Glu Ser Leu Lys Arg Lys Met Thr Arg Leu Ile Ile1 5 10 15Ala Ile Ile Val Val Ser Val Gly Leu Val Phe Leu Leu Gln Ile Leu20 25 30Leu Asn Val Leu Glu Phe Ser Asn Phe Gly Lys Ser Leu Tyr Thr Asn35 40 45Phe Trp Lys Gln Pro His Tyr Ile Phe Ser Phe Thr Ser Ala Cys Ile50 55 60Ser Ile Met Thr Pro Leu Leu Ile Ile Tyr Val Phe Thr Gln Phe Phe65 70 75 80Tyr Lys Lys Tyr Ile Thr Thr Ser Ile Asn Tyr Leu Asn Asp Thr Ile85 90 95Gln Gly Leu Lys Ser Asn Arg Thr Asp Val Glu Tyr Lys Lys Met Ser100 105 110Ile Ser Glu Phe Asn Lys Leu Ile Gln Glu Val Glu His Ile His Thr115 120 125Tyr Leu Ser Asn Ser Ile Lys Asp Gln Leu Ser Ile Gln Asn Asn Phe130 135 140Lys Arg Lys Leu Glu Ile Leu Glu His Asp Ile Lys Thr Pro Leu Thr145 150 155 160Val Leu Gln Gly His Ile Glu Leu Leu Arg Lys Ile Asn Arg Ser Asn165 170 175Leu Ser Gln Glu Glu Ile His Phe Lys Ile Asn Lys Glu Ala Asp Ile180 185 190Ala Leu Gln Ser Ile Asp Arg Ile Asn Asn Gln Leu Gln Ile His Ile195 200 205Lys Asn Ile Asn Leu Leu Pro Asn His Ser Asp Thr Ile His Val Asp210 215 220Lys Val Ile Lys Ile Ile Lys Glu Ser Tyr Asn Gln Gln Tyr Ile Leu225 230 235 240Lys Glu Arg Glu Leu Ile Ile Asp Val Asp His Asp Phe Pro Tyr His
245 250 255Thr Leu Ser Ile Asn Asn Gln Val Leu Tyr His Val Ile Asp Asn Leu260 265 270Ile Asn Asn Ala Leu Lys Tyr Ala Glu Lys Gln Ile Ser Ile Lys Phe275 280 285Glu Val Ile Asn Asp His Leu Leu Asn Phe Thr Ile Thr Asn Asp Gly290 295 300Arg Ile Phe Thr Glu Asp Glu Leu Asn Ser Ala Lys Glu Trp Gly Thr305 310 315 320Lys Gly Lys Glu Ser Asn Gly Ser Gly Ile Gly Leu Tyr Phe Ala Asn325 330 335Ile Val Leu Gln Gln Tyr Asn Ser Glu Leu Ile Leu Glu Asn Glu Asn340 345 350Lys His Ala Lys Val Ser Phe Asn Ile Pro Val355 360<210>3<211>512<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>3Met Lys Thr Tyr Lys Pro Tyr Arg His Gln Leu Arg Arg Ser Leu Phe1 5 10 15Ala Ser Thr Ile Phe Pro Val Phe Met Val Met Ile Ile Gly Leu Ile20 25 30Ser Phe Tyr Ala Ile Tyr Ile Trp Val Glu His Arg Thr Ile His Gln35 40 45His Thr Tyr Gln Thr Gln Thr Glu Leu Gln Arg Ile Asp Lys His Phe50 55 60His Thr Phe Val Thr Gln Gln Gln Lys Gln Trp Arg His Val Asp Leu65 70 75 80Ser His Pro Ala Asp Ile Thr Lys Met Lys Arg Gln Leu Leu Lys Gln85 90 95Val His Gln Gln Pro Ala Ile Leu Tyr Tyr Asp Leu Lys Gly Ser Ser100 105 110Gln Ser Phe Thr Asn Asn Tyr Glu Gln Leu Asp Thr Thr Lys Met Tyr115 120 125Leu Ile Ser Lys Tyr Arg Ile Asp Phe Lys Asp Asp Thr Tyr Ile Leu130 135 140Lys Ile Tyr Met Ser Ser Thr Pro Leu Leu Lys Asn Ile Lys Lys Asn145 150 155 160Ser Gly Gln Ser Ala Leu Ile Val Asp Ser Tyr Asp Thr Val Leu Tyr165 170 175Thr Asn Asp Asp Arg Phe Ser Ile Gly Gln Lys Tyr Gln Pro Pro Gln180 185 190
Phe Gly Phe Met Asn Glu Ser Leu Lys Leu Asn Ser His His Ala His195 200 205Leu Ile Ile Tyr Lys Asp Ile His Glu Thr Ile Glu Asp Gly Ile Ala210 215 220Leu Leu Val Val Met Gly Val Val Leu Ile Leu Leu Val Ile Phe Gly225 230 235 240Tyr Ile Ser Ala Asp Arg Met Ala Lys Arg Gln Ser Glu Asp Ile Glu245 250 255Ala Ile Val Arg Lys Ile Asp Asp Ala Lys Asn Arg His Leu Gly Ser260 265 270Tyr Glu Pro Leu Lys Lys His Ser Glu Leu Glu Glu Ile Asn Asn Tyr275 280 285Ile Tyr Asp Leu Phe Glu Ser Asn Glu Gln Leu Ile Gln Ser Ile Glu290 295 300Gln Thr Glu Arg Arg Leu Arg Asp Ile Gln Leu Lys Glu Ile Glu Arg305 310 315 320Gln Phe Gln Pro His Phe Leu Phe Asn Thr Met Gln Thr Ile Gln Tyr325 330 335Leu Ile Pro Leu Ser Pro Lys Val Ala Gln Thr Val Ile Gln Gln Leu340 345 350Ser Gln Met Leu Arg Tyr Ser Leu Arg Thr Ala Ser His Thr Val Lys355 360 365Leu Ala Glu Glu Leu Ser Tyr Ile Glu Gln Tyr Val Ala Ile Gln Asn370 375 380Ile Arg Phe Asp Asp Met Ile Gln Leu Tyr Ile Asp Ala Thr Glu Gly385 390 395 400Val Gln His Gln Thr Ile Gly Lys Met Met Leu Gln Pro Leu Val Glu405 410 415Asn Ala Ile Lys His Gly Arg Asp Ser Glu Pro Leu Lys Ile Thr Ile420 425 430Arg Ile Arg Leu Thr Lys Arg Lys Leu His Ile Leu Val His Asp Asn435 440 445Gly Ile Gly Met Ser Pro Ser His Leu Glu His Val Arg Gln Ser Leu450 455 460His His Asp Val Phe Asp Thr Thr His Leu Gly Leu Asn His Leu His465 470 475 480Asn Arg Ala Met Ile Gln Tyr Gly Thr Tyr Ala Arg Leu His Ile Phe485 490 495Ser Arg Ser Gln Gln Gly Thr Leu Met Cys Tyr Gln Ile Pro Leu Val500 505 510<210>4<211>346<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)
<400>4Met Asn Asn Phe Arg Trp Phe Trp Phe Phe Ile Lys Ser Arg Ile Asn1 5 10 15Trp Ile Leu Trp Ile Leu Phe Leu Asn Ile Ile Leu Leu Gly Val Ala20 25 30Tyr Ile Asp Tyr Glu Ile Ser Val Glu Ser Val Phe Tyr Ile Val Ile35 40 45Leu Asn Val Gly Leu Ser Ile Leu Phe Leu Leu Phe Thr Phe Val Lys50 55 60Glu Val Arg Leu Ser Lys His Phe Tyr Glu Asp Lys Glu Ile Glu Glu65 70 75 80Ile Lys His Lys Asp Leu Ala Glu Thr Pro Phe Gln Gln Gln Val Ile85 90 95Asp Tyr Leu Tyr Arg His Ile Ala Ala Gln Lys Glu Lys Val Val Glu100 105 110Gln Gln Leu Gln Ile Lys Asn His Glu Gln Thr Ile Thr Glu Phe Val115 120 125His Asp Ile Lys Thr Pro Val Thr Ala Met Lys Leu Leu Ile Asp Gln130 135 140Glu Asn Asp Asp Gln Arg Lys Arg Ala Leu Leu Phe Glu Trp Ser Arg145 150 155 160Ile Asn Glu Met Leu Asp Lys Gln Leu Tyr Leu Thr Arg Leu Glu Thr165 170 175His His Arg Asp Met Tyr Phe Asp Tyr Ile Ser Leu Lys Arg Met Val180 185 190Ile Asp Glu Ile Gln Val Thr Arg His Ile Ser Gln Ala Lys Gly Ile195 200 205Gly Phe Glu Leu Asp Phe Lys Asp Glu Gln Lys Val Tyr Thr Asp Val210 215 220Lys Trp Cys Arg Met Met Ile Arg Gln Val Leu Ser Asn Ser Leu Lys225 230 235 240Tyr Ser Asp Asn Ser Thr Ile Asn Leu Ser Gly Tyr Asn Ile Glu Gly245 250 255His Val Val Leu Lys Ile Lys Asp Tyr Gly Arg Gly Ile Ser Lys Arg260 265 270Asp Leu Pro Arg Ile Phe Asp Arg Gly Phe Thr Ser Thr Thr Asp Arg275 280 285Asn Asp Thr Ala Ser Ser Gly Met Gly Leu Tyr Leu Val Gln Ser Val290 295 300Lys Glu Gln Leu Gly Ile Glu Val Lys Val Asp Ser Ile Val Gly Lys305 310 315 320Gly Thr Thr Phe Tyr Phe Ile Phe Pro Gln Gln Asn Glu Ile Ile Glu325 330 335Arg Met Ser Lys Val Thr Arg Leu Ser Phe
340 345<210>5<211>321<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>5Met Trp Phe Asn Gly His Met Thr Leu Thr Leu Ala Ile Thr Thr Met1 5 10 15Ile Thr Ser Cys Leu Thr Leu Ser Ile Cys Ser Ile Phe Ile Asn Pro20 25 30Leu Ile Gln Lys Ile Lys Gln Phe Asn Ile Lys Thr Lys Gln Phe Ile35 40 45Asn His Glu Lys Phe Ile Asp Asp Glu Thr Phe Gln Ser Pro Arg Glu50 55 60Ile Lys Glu Leu Asn Asp Ser Phe Asn Lys Met Ala Tyr Glu Ile Asn65 70 75 80Asn Gln Met Asn Met Ile Lys Asn Glu Gln Gln Glu Lys Thr Glu Ile85 90 95Ile Gln Asn Leu Ala His Asp Leu Lys Thr Pro Leu Ala Gly Ile Arg100 105 110Ser Tyr Ser Glu Gly Leu Arg Asp Gly Val Ile Ser Asp Pro Gln Glu115 120 125Val His Glu Ala Tyr Glu Ile Leu Ile Lys Gln Ala Asn Arg Leu Ser130 135 140Ile Leu Phe Asp Asp Ile Thr His Val Ile Asn Leu Asn Thr Gly Arg145 150 155 160Ser Tyr Pro Leu Glu Leu Ile Gln Leu Asp Gln Leu Leu Val Asn Ile165 170 175Leu Gln Pro Tyr Glu Gln His Ile Lys Gln Glu Asn Arg Thr Leu Glu180 185 190Val Asn Phe Cys Thr Asp Ile Asp Ala Phe Tyr Gln Tyr Arg Pro Pro195 200 205Ile Glu Arg Ile Leu Thr Asn Leu Leu Asp Asn Ala Leu Lys Phe Ser210 215 220Asn Ser Gly Ser Arg Ile Asp Ile Ile Ile Ser Glu Cys Lys Glu Asn225 230 235 240Asp Val Ile Ser Ile Ser Ile Lys Asp Glu Gly Ile Gly Ile Val Pro245 250 255Glu Leu Gln Ser Arg Ile Phe Glu Arg Thr Phe Arg Val Glu Asp Ser260 265 270Arg Asn Thr Lys Thr Gly Gly Ser Gly Leu Gly Leu Tyr Ile Ala Asn275 280 285Glu Leu Ala Gln Gln Ile Asp Ala Ser Ile Thr Val Gln Ser Asp Leu290 295 300
Asp Ile Gly Thr Thr Met Thr Leu Thr Leu Lys Lys Phe Gln Phe Lys305 310 315 320Lys<210>6<211>243<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>6Met Glu Ser Arg Ser Trp Gly Ala Arg Ile Gly Glu Leu Ser Thr Leu1 5 10 15Ile Tyr Leu Ile Phe Pro Ile Leu Ala Ile Phe Val Asp Lys Arg Gly20 25 30Asn Leu Phe Thr Tyr Leu Ile Val Cys Thr Ile Phe Ile Ile Ser Tyr35 40 45Val Thr Met Ile Leu Phe Tyr Lys His Leu Ser Asp Ser Ile Leu Tyr50 55 60Ser Leu Leu Val Ile His Tyr Leu Gly Ile Phe Tyr Phe Val Tyr Ser65 70 75 80Val Asn Pro Met Asn Ser Leu Phe Phe Phe Tyr Ser Ala Phe Ala Leu85 90 95Pro Phe Ile Phe Asn Val Arg Val Val Ser Lys Glu Phe Ile Thr Phe100 105 110Leu Ile Ala Met Ile Ser Cys Leu Ile Leu Thr Tyr Ile Phe Asn Pro115 120 125Thr Phe Val Val Pro Leu Ser Ala Phe Tyr Leu Val Ile Leu Ile Val130 135 140Ala Val Gly Asn Phe Lys Asn Arg Asp Glu Arg Ile Met Lys Ala Lys145 150 155 160Leu Glu Glu Lys Asn Lys Tyr Ile Asn Val Leu Ile Ala Gln Gln Glu165 170 175Arg Asn Arg Ile Ser Gln Asp Leu His Asp Thr Leu Gly His Val Phe180 185 190Ala Ser Leu Thr Leu Lys Ser Glu Leu Ala Val Lys Leu Ile Asp Thr195 200 205Asn Pro Lys Glu Ala Lys Asn Glu Met Gln Ala Ile Asn Gln Leu Ser210 215 220Thr Glu Ala Leu Asn Lys Val Arg Leu Ile Ile Asp Asp Leu Lys Ile225 230 235 240Pro Ile Phe<210>7<211>456<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>7
Met Ile Lys Arg Gln Lys Leu Lys Tyr Lys Trp Met Leu Ile Thr Thr1 5 10 15Leu Ile Thr Phe Thr Thr Ile Leu Leu Phe Cys Leu Ile Ile Ile Phe20 25 30Phe Leu Lys Asp Thr Leu Arg Ser Ser Glu Ile Asp Glu Ala Glu Arg35 40 45Ser Ser Asn Asp Ile Ala Asn Leu Phe His Ser Lys Ser Leu Ser Asp50 55 60Ile Ser Ala Leu Asp Leu Asn Ala Ser Leu Glu Asn Phe Gln Glu Ile65 70 75 80Leu Ile Tyr Asp Asp Lys Gly Arg Lys Leu Ile Gln Thr Ser Asn Asp85 90 95Asn Thr Leu Ala Tyr Asp Asn Lys Ile Asp Phe Lys His Pro Glu Arg100 105 110Ile His Ile Gln Arg Ser His Gly Ile Asn Tyr Leu Val Ile Thr Glu115 120 125Pro Ile Arg Ser Lys Asp Phe Ser Gly Tyr Ser Val Leu Val His Ser130 135 140Leu Gln Asn Tyr Asp Asn Leu Val Lys Ser Leu Tyr Ile Val Ala Leu145 150 155 160Ala Phe Gly Leu Ile Ala Thr Ile Ile Thr Ala Gly Val Ser Tyr Ile165 170 175Phe Ser Ser Gln Ile Thr Lys Pro Ile Val Thr Met Ser Asn Lys Met180 185 190Asn Gln Ile Arg Arg Asp Gly Phe Gln Asn Lys Leu Glu Leu Thr Thr195 200 205Asn Tyr Glu Glu Thr Asp Asn Leu Ile Asp Thr Phe Asn Glu Met Met210 215 220Tyr Gln Ile Glu Glu Ser Phe Asn Gln Gln Arg Gln Phe Val Glu Asp225 230 235 240Ala Ser His Glu Leu Arg Thr Pro Leu Gln Ile Ile Gln Gly His Leu245 250 255Asn Leu Ile Gln Arg Trp Gly Lys Lys Asp Pro Ala Val Leu Glu Glu260 265 270Ser Leu Asn Ile Ser Ile Glu Glu Val Asn Arg Ile Thr Lys Leu Val275 280 285Glu Glu Leu Leu Leu Leu Thr Lys Asp Arg Val Asn His Asn Val Leu290 295 300Glu Cys Glu Asn Val Asp Val Asn Ser Glu Ile Gln Ser Arg Val Lys305 310 315 320Ser Leu Gln His Leu His Pro Asp Tyr Thr Phe Glu Thr His Leu Ala325 330 335Thr Lys Pro Ile Gln Leu Lys Ile Asn Arg His Gln Phe Glu Gln Leu
340 345 350Leu Leu Ile Phe Ile Asp Asn Ala Met Lys Tyr Asp Thr Glu His Lys355 360 365His Ile Lys Ile Val Thr Gln Leu Lys Asn Lys Met Ile Met Ile Asp370 375 380Ile Thr Asp His Gly Met Gly Ile Pro Lys Ala Asp Leu Glu Phe Ile385 390 395 400Phe Asp Arg Phe Tyr Arg Val Asp Lys Ser Arg Ala Arg Ser Gln Gly405 410 415Gly Asn Gly Leu Gly Leu Ser Ile Ala Glu Lys Ile Val Gln Leu Asn420 425 430Gly Gly Met Ile Gln Val Glu Ser Glu Leu Gln Lys Tyr Thr Thr Phe435 440 445Lys Ile Ser Phe Pro Val Leu Asn450 455<210>8<211>589<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>8Met Met Asn Arg Leu Asn Ser Val Val Ile Lys Leu Trp Leu Thr Ile1 5 10 15Ile Leu Ile Val Ala Thr Val Leu Ile Leu Leu Ser Val Ser Leu Ile20 25 30Thr Phe Ile Gln Tyr Tyr Phe Thr Gln Glu Thr Glu Asn Ala Ile Lys35 40 45Glu Asp Ala Lys Arg Ile Ser Ser Leu Val Glu Lys Ser His Asn Lys50 55 60Thr Leu Ala Ile Gln Asn Ser Gln Thr Leu Ile Asp Gly Asp Gly Pro65 70 75 80Gly Gly Leu Ile Ile Met Lys Asp Met Ser Glu Lys Pro Asp Ser Ser85 90 95Tyr Lys Asp Thr Lys Lys Gln Met Phe Asn Glu Ile Lys Lys Ser Thr100 105 110Lys Phe Lys Lys Val Phe Lys Glu Gly Glu Tyr Glu Thr Gln Asn Ile115 120 125Thr Ile Lys Asn Lys Gly Asn Ser Gln Ser Tyr Leu Leu Leu Gly Tyr130 135 140Pro Met Lys Ala Gln Lys Gly Ala Gln Ser His Tyr Ser Gly Val Phe145 150 155 160Ile Tyr Lys Asp Leu Lys Ser Ile Glu Asp Thr Asn Asn Ala Ile Thr165 170 175Ile Ile Ile Leu Ile Thr Ala Ile Ile Phe Thr Ile Ala Ser Thr Ile180 185 190
Phe Ala Phe Phe Leu Ser Asn Arg Ile Thr Lys Pro Leu Arg Gln Leu195 200 205Lys Thr Gln Ala Gln Lys Val Ser Glu Gly Asp Tyr Ser Gln Ile Ser210 215 220Thr Val Ala Thr Lys Asp Glu Ile Gly Asp Leu Ser Arg Ala Phe Asn225 230 235 240Asn Met Asn Val Glu Ile Gln Glu His Ile Lys Ala Ile Ser Ser Ser245 250 255Lys Asn Ile Arg Asp Thr Leu Leu Asn Ser Met Val Glu Gly Val Leu260 265 270Gly Ile Asn Asn Gln Arg Glu Ile Ile Leu Ser Asn Lys Met Ala Asp275 280 285Asp Ile Met Arg His Ile Asp Asp Phe Ser Lys Glu Ser Ile Glu Gln290 295 300Gln Ile Glu Ala Thr Phe Glu Ser Gln Gln Asn Glu Tyr Leu Glu Leu305 310 315 320Glu Ile Asn Thr Arg Tyr Tyr Val Phe Ile Ser Ser Tyr Ile Asp Arg325 330 335Ile Gln Thr Asn Gly Arg Ser Gly Ile Val Met Val Ile Arg Asp Met340 345 350Thr Asn Glu His Asn Leu Asp Gln Met Lys Lys Asp Phe Ile Ala Asn355 360 365Val Ser His Glu Leu Arg Thr Pro Ile Ser Leu Leu Gln Gly Tyr Thr370 375 380Glu Ser Ile Val Asp Gly Ile Val Thr Glu Pro Asp Glu Ile Arg Asp385 390 395 400Ser Leu Ala Ile Val Leu Asp Glu Ser Lys Arg Leu Asn Arg Leu Val405 410 415Asn Glu Leu Leu Asn Val Ala Arg Met Asp Ala Glu Gly Leu Ser Val420 425 430Glu Lys Glu Leu Gln Pro Ile Gln His Leu Leu Asp Lys Met Glu Ser435 440 445Lys Tyr Arg Met Gln Ser Glu Glu Leu Gly Leu Thr Met Thr Phe Asp450 455 460Ser Asn Asn Asp Glu Gln Leu Trp Asn Tyr Asp Met Asp Arg Met Asp465 470 475 480Gln Val Leu Thr Asn Leu Ile Asp Asn Ala Thr Arg Tyr Thr Gln Ala485 490 495Gly Asp Ser Ile Lys Ile Ser Ile Asp Glu Asp Ser Asp Phe Asn Ile500 505 510Leu Thr Ile Thr Asp Thr Gly Thr Gly Ile Ala Pro Glu His Leu Lys515 520 525Gln Val Phe Asp Arg Phe Tyr Lys Val Asp Ala Ala Arg Lys Arg Gly530 535 540
Lys Gln Gly Thr Gly Leu Gly Leu Phe Ile Cys Lys Met Ile Ile Glu545 550 555 560Glu His Gly Gly Arg Ile Asp Val Glu Ser Glu Leu Gly Lys Gly Thr565 570 575Ser Phe Ile Ile Arg Leu Pro Lys Ser Lys Gln Ile Ser580 585<210>9<211>565<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>9Met Leu Lys Phe His Leu Arg Leu Leu Leu Leu Ile Ser Thr Ile Thr1 5 10 15Ile Ile Ser Phe Ile Gly Leu Gly Ala Ile Ile His Asn Thr Ile Tyr20 25 30Gln Thr Leu Thr Ser Asn Gln Ile Lys Ser Leu Asp Ser Glu Ala Arg35 40 45Asn Tyr Val Asn Leu Phe Asn Asn Asn Lys Glu Lys Glu Ile Thr Asn50 55 60Ile Ala His Asn Glu Lys Asn Ile Ile Leu Ile Lys Glu Lys Asp Lys65 70 75 80Asp Lys Ile Ile Tyr Ser Ser Gly Asn Ile Lys Asp Ile Asp His Arg85 90 95Ile Asp Asn Glu Ala Asn Pro Ser Lys Leu Ile Asn Lys Asn Thr Lys100 105 110Leu Gly Met Arg Tyr Thr Tyr Lys Asn Thr Ile Asp Asp Lys Thr Ile115 120 125Tyr Ile Ser Gly Ile Asn Asn Glu Ile Ile Asp Leu Gln Lys Asp Leu130 135 140Trp Lys Tyr Leu Ser Ile Val Gly Val Ile Val Leu Phe Thr Val Tyr145 150 155 160Leu Ala Ser Arg Ser Ile Asn Arg Thr Tyr Ile Arg Pro Ile Asn Glu165 170 175Val Thr Tyr Ala Thr Ser Leu Leu Ala Asp Gly Tyr Tyr His Val Arg180 185 190Val Pro Glu Ser Asn Val Lys Glu Thr Arg Ala Leu Phe Val Thr Thr195 200 205Asn Asp Leu Ala Arg Arg Leu Gln Lys Leu Asn Asn Ser Gln Lys Ile210 215 220Gln Ser Asn Arg Leu Lys Thr Thr Leu Glu Asn Ile Pro Ser Ser Val225 230 235 240Leu Met Ile Asp Lys His Gly Glu Ile Val Val Ala Asn His Ala Tyr245 250 255Tyr Gln Val Phe Asn Pro Asp Gln Met Val Glu Asn Lys Ser Tyr Ile
260 265 270Gly Phe Ile Asp Asp Ser Ile Glu Lys Leu Ile Ile Glu Ser Phe Arg275 280 285Thr Glu Lys Val Ile Tyr Glu Gln Leu Glu Val Ala Ile Asn Asn Val290 295 300His Thr Lys Tyr Phe Asp Val Ser Cys Ile Pro Ile Leu Thr Lys Ser305 310 315 320Lys Lys Asn Leu Gln Gly Met Val Val Val Leu His Asp Ile Thr Asn325 330 335Leu Gln Lys Leu Glu Asn Leu Arg Arg Glu Phe Val Ala Asn Val Ser340 345 350His Glu Leu Lys Thr Pro Ile Thr Ser Ile Lys Gly Phe Ala Glu Thr355 360 365Leu Ile Glu Gly Ala Lys Asn Asp Glu Gln Ser Leu Asp Met Phe Leu370 375 380Asn Ile Ile Leu Lys Glu Ser Asn Arg Ile Glu Ser Leu Val Thr Asp385 390 395 400Leu Leu Asp Leu Ser His Ile Glu Gln Gln Lys Glu Leu Glu Ile Asn405 410 415Tyr Met Asn Leu Ser Glu Leu Ala Ile Asn Ile Ile Asp Asn Leu Gln420 425 430Thr Gln Ala Tyr Asn Lys Arg Ile Lys Ile Gln Ser Glu Ile Glu Lys435 440 445Asp Val Ile Ile Glu Ala His Glu Asn Lys Ile Ala Gln Val Ile Thr450 455 460Asn Leu Leu Ser Asn Ala Ile Asn Tyr Ser Ser Glu Asp Asn Lys Val465 470 475 480Ile Val Arg Val Tyr Arg Asn Asp Asn Lys Val Tyr Leu Glu Ile Gln485 490 495Asp Tyr Gly Ile Gly Ile Ser Glu Thr Asp Gln Lys Arg Ile Phe Glu500 505 510Arg Phe Tyr Arg Val Asp Lys Ala Arg Ser Arg Asp Ser Gly Gly Thr515 520 525Gly Leu Gly Leu Ser Ile Thr Lys His Ile Val Glu Ala His Asn Gly530 535 540Arg Ile Asp Val Lys Ser Ala Pro Gly Lys Gly Ser Ile Phe Lys Val545 550 555 560Leu Phe Asn Asp Asn565<210>10<211>377<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>10
Met Glu Lys Pro Thr Arg Leu Ala Leu Leu Lys Glu Ile Ala Glu Phe1 5 10 15Leu Asn Glu Glu Thr Glu Ile Tyr Ser Met Met Gln Gly Ala Leu Arg20 25 30Leu Leu Ile Asn Gly Ser Asp Phe Ser Thr Gly Trp Ile Phe Tyr Ile35 40 45Asp Glu Glu Gly His His Asp Leu Val Ser Ser Val Asp Leu Pro Glu50 55 60Ala Leu Ser Lys Asn His Cys Tyr Tyr Leu Thr Asn Gly Ser Cys Trp65 70 75 80Cys Val Gln Ala Tyr Gln Asn His Lys Leu Thr Lys Ala Ser Asn Ile85 90 95Ile Asn Cys Ser Arg Ile Asn Leu Ala Asn His Ala Tyr Asp Lys Glu100 105 110Thr Asn His Ile Thr His His Ala Thr Val Pro Leu Gln Ser Gly Ser115 120 125Glu Arg Phe Gly Leu Leu Asn Val Ala Ser Pro Asn Lys Glu His Tyr130 135 140Ser Glu Glu Asp Leu Glu Leu Leu Glu Ser Val Ala Phe Gln Ile Gly145 150 155 160Ser Ala Ile Lys Arg Ile Gln Leu Thr Asn Lys Glu Lys Glu Asn Ala165 170 175Lys Ile Asn Glu Arg Asn Arg Leu Ala Arg Asp Leu His Asp Ser Val180 185 190Asn Gln Met Leu Phe Ser Leu Lys Leu Thr Ala His Ala Ala Lys Gly195 200 205Ile Thr Thr Asp Glu Gln Ala Gln Arg Ala Phe Lys Thr Ile Glu Glu210 215 220Thr Ser Gln Asn Ala Val Asn Glu Met Arg Ala Leu Ile Trp Gln Leu225 230 235 240Lys Pro Val Gly Leu Glu Gln Gly Leu Ile His Ala Leu His Tyr Tyr245 250 255Ser Lys Met Leu Gly Leu Asp Leu Lys Val Thr Ile Asn Gly Leu Ile260 265 270Asp Leu Ser Asn Asp Ile Glu Glu Asn Val Tyr Arg Ile Ile Gln Glu275 280 285Ala Met Asn Asn Val Ile Lys His Ala Lys Thr Asn Lys Val Tyr Leu290 295 300Asp Leu Ser Gln Asn Val Asp Tyr Leu Glu Val Asp Ile Arg Asp Asn305 310 315 320Gly Gln Gly Phe Asp Thr Gln Ser Ile Asn Thr Phe Leu Phe Asn Gly325 330 335Leu Lys Asn Ile Lys Gln Arg Thr Gln Tyr Leu Asn Gly Lys Val Thr340 345 350
Ile Glu Ser Lys Ile Asn Gln Gly Thr Arg Ile Tyr Ile Lys Ile Pro355 360 365Leu Lys Leu Lys Glu His Pro Tyr Ala370 375<210>11<211>348<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>11Met Asn His Tyr Ile Arg Ala Ile Gly Ser Met Leu IIe Leu Val Tyr1 5 10 15Ser Met Leu Ile Ala Phe Leu Phe Ile Asp Lys Val Phe Val Asn Ile20 25 30Ile Phe Phe Gln Gly Met Phe Tyr Thr Gln Ile Phe Gly Ile Pro Val35 40 45Phe Leu Phe Leu Asn Leu Leu Ile Val Leu Leu Cys Ile Ile Val Gly50 55 60Ser Val Leu Ala Tyr Lys Ile Asn Gln Gln Asn Asp Trp Ile Ile Ser65 70 75 80Gln Ile Glu Arg Ser Ile Glu Gly Gln Thr Val Gly Ile Asn Asp Gln85 90 95Asn Ile Glu Leu Tyr Thr Glu Thr Ile Asp Ile Tyr His Thr Leu Val100 105 110Pro Leu Asn Gln Glu Leu His Arg Leu Arg Met Lys Thr Gln Asn Leu115 120 125Thr Asn Glu Asn Tyr Asn Ile Asn Asp Val Lys Val Lys Lys Ile Ile130 135 140Glu Asp Glu Arg Gln Arg Leu Ala Arg Glu Leu His Asp Ser Val Ser145 150 155 160Gln Gln Leu Phe Ala Ala Ser Met Met Leu Ser Ala Ile Lys Glu Ser165 170 175Lys Leu Glu Pro Pro Leu Asn Gln Gln Ile Pro Ile Leu Glu Lys Met180 185 190Val Gln Asp Ser Gln Leu Glu Met Arg Ala Leu Leu Leu His Leu Arg195 200 205Pro Ile Gly Leu Lys Asp Lys Ser Leu Gly Glu Gly Ile Lys Asp Leu210 215 220Val Ile Asp Leu Gln Lys Lys Val Pro Met Lys Val Val His Glu Ile225 230 235 240Gln Asp Phe Glu Val Pro Lys Gly Ile Glu Asp His Leu Phe Arg Ile245 250 255Thr Gln Glu Ala Ile Ser Asn Thr Leu Arg His Ser Asn Gly Thr Lys260 265 270Val Thr Val Glu Leu Phe Asn Gln Glu Asp Tyr Leu Leu Leu Arg Ile
275 280 285Gln Asp Asn Gly Lys Gly Phe Asn Val Asp Glu Lys Phe Glu Gln Ser290 295 300Tyr Gly Leu Lys Asn Met Arg Glu Arg Ala Leu Glu Ile Gly Ala Thr305 310 315 320Phe His Ile Val Ser Leu Pro Asp Ser Gly Thr Arg Ile Glu Val Lys325 330 335Ala Pro Leu Asn Lys Glu Glu Asn Ser Ser Gly Asp340 345<210>12<211>429<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>12Met Asp Asp Ile Asn Leu Phe Pro Phe Ala Gly Leu Gln Ile Phe Leu1 5 10 15Met Ile Trp Val Thr Lys Val Ile Ile Asn Met Lys Phe Asn Phe Arg20 25 30Asp Tyr Ile Ile Val Phe Thr Ile Val Ile Pro Ser Ala Ile Met Tyr35 40 45Tyr Phe Trp Gln Ser Lys Ala Leu Ile Val Leu Val Ile Ile Ile Thr50 55 60Ile Phe Phe Tyr Thr Lys Ile Lys Leu Tyr Ser Ile Leu Val Val Leu65 70 75 80Phe Thr Thr Met Ile Leu Tyr Ile Thr Asn Phe Ile Thr Val Tyr Ile85 90 95His Leu Thr Ile Lys Asp Tyr Ile Pro Phe Lys Phe Ala Leu Gln Leu100 105 110Ile His Phe Thr Ser Phe Val Ile Ile Thr Leu Ile Ile Ala Tyr Leu115 120 125Thr Gln Leu Leu Phe Asn Lys Leu Lys Val Ser Tyr Leu Ser Leu Asn130 135 140Lys Arg Tyr Leu Leu Ile Ile Thr Ile Val Leu Phe Ile Ser Phe Ile145 150 155 160Leu Leu Tyr Met Val Ser Gln Thr Asp Met Arg Gly Asn Asp Thr Leu165 170 175Lys Leu Tyr Ala Ile Leu Leu Leu Gly Ile Met Val Phe Leu Ser Val180 185 190Val Ile Leu Val Met Ser Asn Phe Thr Leu Arg Glu Met Arg Tyr Lys195 200 205Arg Asn Val Lys Glu Ile Glu Ala Tyr Tyr Glu Tyr Thr Leu Arg Ile210 215 220Glu Ser Ile Asn Asn Glu Met Arg Lys Phe Arg His Asp Tyr Val Asn225 230 235 240
Ile Leu Thr Thr Leu Ser Asp Tyr Ile Arg Glu Asp Asp Met Pro Gly245 250 255Leu Arg Lys Tyr Phe Asn Glu Asn Ile Val Pro Met Lys Asp Lys Leu260 265 270Lys Thr Arg Ser Ile Lys Met Asn Gly Ile Glu Lys Leu Lys Val Arg275 280 285Glu Ile Lys Gly Leu Ile Thr Thr Lys Ile Ile Gln Ala Gln Glu Lys290 295 300Arg Ile Pro Ile Ser Ile Glu Val Pro Asp Glu Ile Asp Arg Ile Asp305 310 315 320Met Asn Thr Val Glu Leu Ser Arg Ile Ile Gly Ile Ile Val Asp Asn325 330 335Ala Ile Glu Ala Ser Glu Asn Leu Glu Glu Pro Leu Ile Asn Ile Ala340 345 350Phe Ile Asp Asn Glu Glu Ser Val Thr Phe Ile Val Met Asn Lys Cys355 360 365Ser Asn Asp IlePro Lys Ile His Glu Leu Phe Glu Gln Gly Phe Ser370 375 380Thr Lys Gly Asp Asn Arg Gly Leu Gly Leu Ser Thr Leu Lys Glu Leu385 390 395 400Thr Asp Ser Asn Glu Asn Val Leu Leu Asp Thr Val Ile Glu Asn Gly405 410 415Tyr Phe Val Gln Lys Val Glu Ile Asn Asn Lys Glu Ser420 425<210>13<211>451<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>13Met Phe Lys Thr Leu Tyr Ser Arg Ile Ala Ile Tyr Ala Ile Thr Val15 10 15Ile Leu Phe Ser Ala Leu Met Ser Phe Leu Phe Thr Asn Ile Tyr Tyr20 25 30His Phe His Leu Lys Ala Ser Asn Asp Ala Lys Ile Met Arg Thr Leu35 40 45Lys Glu Ala Arg Glu Tyr Glu Arg Thr Gln Lys Pro Lys Pro Leu Asp50 55 60Thr Tyr Leu Lys His Leu Gly Gln Met Asn Tyr Gln Ile Met Thr Val65 70 75 80Asn Glu His Gly Thr Lys His Phe Tyr Gly Glu Thr Phe Arg Lys Asn85 90 95Thr Ile Ser Gln Ser Ala Ile Lys Lys Val Leu Asn Gly Glu Asp Tyr100 105 110His Gly Ile Lys Asn Lys Pro Tyr Ala Phe Phe Val Thr Gly Phe Phe
115 120 125Asp Asn Glu Thr Asp Asn Thr Val Gly Ile Gln Phe Lys Thr Asp Asp130 135 140Gly Ala Leu Ala Val Phe Met Arg Pro Asp Ile Gly Lys Thr Phe Ser145 150 155 160Glu Phe Arg Ile Phe Leu Ala Val Leu Ile Thr Leu Leu Leu Ile Ile165 170 175Ser Ile Ser Leu Val Ile Ala Ser Thr Tyr Ser Ile Ile Lys Pro Val180 185 190Thr Ala Leu Lys Asn Ala Thr Thr Arg Ile Met Lys Gly Asp Phe Ser195 200 205Thr Pro Ile Lys Gln Thr Arg His Asp Glu Ile Gly Thr Leu Gln Ser210 215 220Arg Phe Asn Thr Met Arg Gln Asn Leu Gly Gln Val Asp Gln Met Arg225 230 235 240Gln His Phe Val Gln Asn Val Ser His Glu Ile Lys Thr Pro Leu Thr245 250 255His Leu Gln Arg Leu Leu Thr Gln Leu Glu Leu Thr Gln Asn Glu Glu260 265 270Glu Lys Gln Leu Cys Ile Asn Glu Met Phe Glu Ile Thr Asn Gln Val275 280 285Ser Glu Leu Thr Lys Glu Leu Leu Leu Leu Ser Glu Leu Asp Asn Ala290 295 300Ser His Leu Thr Phe Asn Asp Asn Val His Leu Asn Thr Leu Ile Lys305 310 315 320Asp Ile Ile Arg His Glu Gln Phe Arg Thr Asp Glu Lys Asp Leu Val325 330 335Leu Phe Thr Glu Leu Glu Asp Leu Tyr Phe Arg Gly Asn Glu Arg Leu340 345 350Leu His Gln Ala Phe Asn Asn Leu Ile Ile Asn Ala Met Lys Tyr Ala355 360 365Pro Gln Asn Ser Met Ile Asn Ile Thr Leu Thr Ser Thr Asn His Leu370 375 380Ile Ile Phe Asn Ile Glu Asn Asp Gly Ser Ile Ala Glu Glu Asp Ala385 390 395 400Lys His Ile Phe Asp Arg Phe Tyr Lys Leu Ser Asp Glu Ser Ser Ser405 410 415Asn Gly Leu Gly Leu Ala Ile Thr Gln Ser Ile Ile His Leu His His420 425 430Gly Ser Ile Thr Leu Thr Ser Asp Asp Lys Thr Gln Phe Ile Val Lys435 440 445Leu Phe Ile450<210>14
<21l>344<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>14Met Leu Glu Gln Thr Asp Leu Ser Leu Glu Gln Leu Leu Lys Asn Tyr1 5 10 15Tyr Glu Thr Thr Asn Glu Lys Ile Val Phe Val Asn Arg Gln Gly Lys20 25 30Ile Ile Ala Met Asn Asp Ala Ala Lys Asp Ile Leu Thr Glu Glu Asp35 40 45Asn Tyr Asn Ala Met Thr Asn Ala Ile Cys His Arg Cys Glu Gly Tyr50 55 60Ser Asn Glu Tyr Asp Val Gln Ser Cys Lys Asp Cys Phe Leu Glu Thr65 70 75 80Thr Gln Leu Gln His Ser Asn Phe Gln Val Phe Met Lys Thr Lys Asp85 90 95Asn Glu Ile Lys Pro Phe Thr Ala Met Tyr Gln Asn Ile Asp Glu Gln100 105 110Arg Gly Ile Ser Ala Phe Thr Leu Gln Asn Val Ala Pro Gln Ile Glu115 120 125Arg Gln Glu Lys Met Tyr Gln Gln Lys Met Leu His Arg Ser Ile Gln130 135 140Ala Gln Glu Asn Glu Arg Lys Arg Ile Ser Arg Glu Leu His Asp Ser145 150 155 160Val Ile Gln Asp Met Leu Asn Ile Asp Val Glu Leu Arg Leu Leu Lys165 170 175Tyr Lys His Arg Asp Lys Val Leu Ala Glu Thr Ser Gln Arg Ile Glu180 185 190Gly Leu Leu Ser Gln Leu Ile Asp Asp Ile Arg Asn Met Ser Val Glu195 200 205Leu Arg Pro Ser Ser Leu Asp Asp Leu Gly Ile Glu Ala Ala Phe Lys210 215 220Ser Tyr Phe Lys Gln Phe Glu Glu Asn Tyr Gly Met His Ile Lys Tyr225 230 235 240Asp Ser Asn Ile Lys Gly Met Arg Phe Asp Asn Glu Ile Glu Thr Val245 250 255Val Tyr Arg Val Val Gln Glu Gly Val Phe Asn Ala Leu Lys Tyr Ala260 265 270Glu Val Asn Glu Ile Glu Val Ser Thr His Ser Asp Gly Lys Gln Leu275 280 285Val Ala Glu Val Val Asp Arg Gly Lys Gly Phe Ser Leu Asp His His290 295 300Pro Lys Gly Ser Gly Leu Gly Leu Tyr Gly Met Arg Glu Arg Ala Glu305 310 315 320
Leu Val Asn Gly His Val Asn Ile Glu Thr His Ile Asn Arg Gly Thr325 330 335Ile Ile Thr Leu Asp Ile Pro Ile340<210>15<211>591<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>15Met Leu Asn Leu Phe Ile Leu Leu Leu Glu Arg Val Gly Leu Ile Ile1 5 10 15Leu Leu Ala Tyr Ile Leu Met Asn Ile Asn His Phe Lys Thr Met Met20 25 30Ser Glu Arg Asp Lys Trp Arg Ser Lys Phe Gln Leu Ile Ile Ile Phe35 40 45Gly Ile Phe Ser Met Ile Ser Asn Phe Thr Gly Ile Glu Ile Glu Asn50 55 60Gly His Ile Val Ser Ser Asp Ile Tyr Tyr His Leu Ser Lys Asp Ala65 70 75 80Ser Met Ala Asn Thr Arg Val Leu Thr Ile Gly Val Ser Gly Leu Ile85 90 95Gly Gly Pro Trp Val Ala Ile Ile Val Gly Ile Ile Ser Gly Leu Cys100 105 110Arg Leu Tyr Ile Gly Gly Ala Asp Ala Tyr Thr Tyr Leu Ile Ser Ser115 120 125Ile Val Ile Ala Ile Ile Ser Gly Tyr Phe Gly His Gln Thr Ile Lys130 135 140Gln Asn Thr Tyr Pro Ser Ile Lys Lys Gly Ala Ile Ile Gly Ala Ile145 150 155 160Thr Glu Ile Ile Gln Met Gly Cys Ile Leu Leu Phe Thr Asn Asn Leu165 170 175His His Ala Ile Thr Leu Val Ser Phe Ile Ala Leu Pro Met Ile Ile180 185 190Ile Asn Ser Leu Gly Thr Ala Ile Phe Leu Thr Ile Ile Leu Ser Thr195 200 205Ile Lys Gln Glu Glu Gln Met Arg Ala Val Gln Thr His Asp Val Leu210 215 220Gln Leu Ala Asn Glu Thr Leu Pro Tyr Phe Arg Ser Gly Leu Asn Glu225 230 235 240Lys Ser Ala Gln Gln Ala Ala Glu Ile Ile Leu Lys Leu Met Gln Val245 250 255Ser Ala Val Ala Ile Thr Asn Lys Lys Asp Ile Leu Thr His Ile Gly260 265 270Ala Gly Ser Asp His His Val Ala Arg Lys Glu Ile Ile Thr Asp Leu
275 280 285Ser Lys Glu Val Ile Gln Ser Gly Lys Leu Lys Val Ala His Thr Arg290 295 300Glu Gly Ile Gly Cys His His Pro Asn Cys Pro Leu Glu Gly Ala Ile305 310 315 320Val Val Pro Leu Tyr Ile His Asn Glu Val Ala Gly Thr Leu Lys Phe325 330 335Tyr Phe Thr Asp Asn Asn Ile Ile Ser Thr Ser Asp Gln Gln Leu Ala340 345 350Lys Gly Leu Ala Asn Ile Phe Ser Ser Gln Leu Glu Leu Gly Gln Ala355 360 365Glu Met Gln Gly Gln Leu Leu Lys Asp Ala Glu Ile Lys Ser Leu Gln370 375 380Ala Gln Val Asn Pro His Phe Phe Phe Asn Ala Ile Asn Thr Ile Ser385 390 395 400Ala Leu Val Arg Ile Asp Ser Glu Lys Ala Arg Arg Leu Leu Ile Gln405 410 415Leu Ser Gln Phe Phe Arg Ser Asn Leu Asn Gly Ala Arg Asn Asn Thr420 425 430Ile Thr Leu Gln Lys Glu Leu Gln Gln Val Ala Ala Tyr Leu Ser Leu435 440 445Glu Gln Ala Arg Tyr Pro Asn Arg Phe Asn Ile His Tyr Arg Ile Asp450 455 460Asp Gln Cys Gln Asp Ala Leu Ile Pro Pro Phe Ile Ile Gln Ile Leu465 470 475 480Val Glu Asn Ser Ile Lys His Ala Phe Lys Asn Arg Lys Lys Asn Asn485 490 495His Ile Asp Val Asp Val Ser Met Lys Gln Asp Cys Leu Ser Ile Ser500 505 510Val Gln Asp Asn Gly Gln Gly Ile Pro Ala Asp Gln Leu Asp Thr Ile515 520 525Gly Tyr Thr Thr Val Thr Ser Thr Thr Gly Thr Gly Asn Ala Leu Val530 535 540Asn Leu Asn Lys Arg Leu Thr Gly Leu Phe Gly Thr Thr Ser Ala Leu545 550 555 560Asn Ile Gln Ser Ser Gln Ser Gly Thr Thr Val Ser Cys Leu Ile Pro565 570 575Tyr Lys Ser Ser Lys Glu Glu His Phe Asn Glu Ser Val Asn Arg580 585 590<210>16<211>298<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>16
Met Lys Phe Ala Tyr Ile Gln Ser Ile Arg Asn Glu Ile Ser Ile Ile1 5 10 15Leu Ile Ile Leu Leu Phe Phe Ala Leu Ile Phe Tyr Val Phe Ser Leu20 25 30Pro Phe Asp Ala Tyr Val Leu Ala Ile Ser Ile Ile Leu Leu Leu Met35 40 45Cys Val Arg Trp Trp Ile Lys Tyr Leu Ser Phe Lys Lys Asn Glu His50 55 60Leu Lys Asp Lys Val Ala Tyr Leu Glu His Glu Leu Ala His Val Lys65 70 75 80Asn Gln Gln Ile Glu Tyr Arg Asn Asp Val Glu Ser Tyr Phe Leu Thr85 90 95Trp Val His Gln Ile Lys Thr Pro Ile Thr Ala Ser Gln Leu Leu Leu100 105 110Glu Arg Ash Glu Glu Asn Val Val Asn Arg Val Arg Gln Glu Ile Val115 120 125His Ile Asp Asn Tyr Thr Ser Leu Ala Leu Ser Tyr Leu Lys Leu Leu130 135 140Asn Glu Glu Ser Asp Met Thr Ile Thr Lys Val Thr Val Asp Asp Leu145 150 155 160Ile Arg Pro Leu Ile Leu Lys Tyr Arg Ile Gln Phe Ile Glu Gln Lys165 170 175Thr Gln Ile His Tyr Glu Lys Ser Glu Asp Ile Ile Leu Thr Asp Ala180 185 190Gln Trp Ala Ser Ile Met Ile Glu Gln Leu Leu Asn Asn Ala Leu Lys195 200 205Tyr Ala Lys Gly Lys Asp Ile Trp Ile Asp Phe Asp Val Ala Asn Gln210 215 220Thr Leu Gln Ile Lys Asp Asn Gly Ile Gly Ile Ser Lys Ala Asp Ile225 230 235 240Pro Lys Ile Phe Asp Lys Gly Tyr Ser Gly Phe Asn Gly Arg Leu Asn245 250 255Glu Gln Ser Thr Gly Ile Gly Leu Phe Ile Val Gln His Ile Ala Asn260 265 270His Leu Asn Ile Gln Val Thr Val Gln Ser Glu Leu Asn His Gly Thr275 280 285Val Phe Phe Ile His Phe Thr Lys Glu Lys290 295<210>17<211>233<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>17Met Ala Arg Lys Val Val Val Val Asp Asp Glu Lys Pro Ile Ala Asp
1 5 10 15Ile Leu Glu Phe Asn Leu Lys Lys Glu Gly Tyr Asp Val Tyr Cys Ala20 25 30Tyr Asp Gly Asn Asp Ala Val Asp Leu Ile Tyr Glu Glu Glu Pro Asp35 40 45Ile Val Leu Leu Asp Ile Met Leu Pro Gly Arg Asp Gly Met Glu Val50 55 60Cys Arg Glu Val Arg Lys Lys Tyr Glu Met Pro Ile Ile Met Leu Thr65 70 75 80Ala Lys Asp Ser Glu Ile Asp Lys Val Leu Gly Leu Glu Leu Gly Ala85 90 95Asp Asp Tyr Val Thr Lys Pro Phe Ser Thr Arg Glu Leu Ile Ala Arg100 105 110Val Lys Ala Asn Leu Arg Arg His Tyr Ser Gln Pro Ala Gln Glu Val115 120 125Ser Gly Ala Thr Asn Glu Ile Thr Ile Lys Asp Ile Val Ile Tyr Pro130 135 140Asp Ala Tyr Ser Ile Lys Lys Arg Gly Glu Asp Ile Glu Leu Thr His145 150 155 160Arg Glu Phe Glu Leu Phe His Tyr Leu Ser Lys His Met Gly Gln Val165 170 175Met Thr Arg Glu His Leu Leu Gln Thr Val Trp Gly Tyr Asp Tyr Phe180 185 190Gly Asp Val Arg Thr Val Asp Val Thr Ile Arg Arg Leu Arg Glu Lys195 200 205Ile Glu Asp Asp Pro Ser His Pro Glu Tyr Ile Val Thr Arg Arg Gly210 215 220Val Gly Tyr Phe Leu Gln Gln His Asp225 230<210>18<211>222<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>18Met Met Tyr Asn Ile Met Ile Val Asp Asp Glu Glu Lys Ile Val Ser1 5 10 15Phe Ile Glu Glu Ser Leu Tyr Leu Glu Gly Phe Asn Thr Ile Val Ala20 25 30Tyr Asn Gly Arg Asp Val Leu Lys Gln Ile Asp Asp Lys Ile Asp Leu35 40 45Ile Ile Leu Asp Ile Lys Met Pro Tyr Ile Asp Gly Phe Glu Val Ala50 55 60Gln Ser Leu Lys Glu Ser Asn Ile Pro Ile Ile Phe Leu Thr Ala Lys65 70 75 80
Asp Asn Leu Asp Thr Arg Leu Lys Cys Phe Ser Leu Gly Ala Lys Asp85 90 95Tyr Leu Pro Lys Pro Phe Tyr Met Glu Glu Leu Ile Ile Arg Ile Lys100 105 110Asn Val Leu Asn Gln Ser His Ser Gln Ser Leu Thr Pro Asn Ile Lys115 120 125Lys Thr Lys Glu Leu Ile Ile Tyr Tyr Asp Thr Tyr Arg Ile Asp Val130 135 140Asn Asn Met Thr Ile Asp Ile Thr Lys Lys Glu Phe Glu Ile Ile Lys145 150 155 160Leu Leu Ser Leu Asn Pro Gln Tyr Tyr Phe Ser Lys Glu Gln Ile Tyr165 170 175Asp Ser Ile Asn Leu Asn Lys Thr Gly Asn Thr Gln Val Val Ala Glu180 185 190His Ile Arg Lys Ile Arg Lys Lys Leu Ser Cys Tyr Ser Arg Phe Glu195 200 205Tyr Ile Asp Thr Lys Trp Gly Val Gly Tyr Lys Trp Leu Glu210 215 220<210>19<211>251<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>19Met Phe Lys Val Val Ile Cys Asp Asp Glu Arg Ile Ile Arg Gln Gly1 5 10 15Leu Lys Gln Met Ile Pro Trp Lys Glu Tyr His Phe Thr Thr Ile Tyr20 25 30Thr Ala Thr Asp Gly Val Glu Ala Leu Ser Leu Ile Arg Gln His Gln35 40 45Pro Glu Leu Val Ile Thr Asp Ile Arg Met Pro Arg Lys Asn Gly Val50 55 60Asp Leu Leu Asp Asp Ile Lys Asp Leu Asp Cys Gln Val Ile Ile Leu65 70 75 80Ser Ser Tyr Asp Asp Phe Glu Tyr Met Lys Ala Gly Ile Gln His His85 90 95Val Leu Asp Tyr Leu Leu Lys Pro Val Asp His Thr Gln Leu Glu His100 105 110Ile Leu Asp Ile Leu Val Gln Arg Leu Leu Glu Arg Pro His Ser Thr115 120 125Asn Asp Asp Ala Ala Tyr Tyr Thr Ala Phe Gln Pro Leu Leu Lys Ile130 135 140Asp Tyr Asp Asp Tyr Tyr Val Asn Gln Ile Leu Ser Gln Ile Lys Gln145 150 155 160His Tyr His Lys Lys Val Thr Val Leu Asp Leu Ile Asn Pro Ile Val
165 170 175Val Ser Glu Ser Tyr Ala Met Arg Thr Phe Lys Glu His Val Gly Ile180 185 190Thr Ile Val Asp Tyr Leu Asn Arg Tyr Arg Ile Leu Lys Ser Leu His195 200 205Leu Leu Asp Gln His Tyr Lys His Tyr Glu Ile Ala Glu Lys Val Gly210 215 220Phe Ser Glu Tyr Lys Met Phe Cys Tyr His Phe Lys Lys Tyr Leu His225 230 235 240Met Ser Pro Ser Asp Tyr Asn Lys Leu Ser Lys245 250<210>20<211>224<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>20Met Gln Ile Leu Leu Val Glu Asp Asp Asn Thr Leu Phe Gln Glu Leu1 5 10 15Lys Lys Glu Leu Glu Gln Trp Asp Phe Asn Val Val Gly Val Glu Asp20 25 30Phe Ser His Val Met Glu Thr Phe Glu Thr Phe Asn Pro Glu Ile Val35 40 45Ile Leu Asp Val Gln Leu Pro Lys Tyr Asp Gly Phe Tyr Trp Cys Arg50 55 60Lys Met Arg Gln Gln Ser Asn Val Pro Ile Leu Phe Leu Ser Ser Arg65 70 75 80Asp Asn Pro Met Asp Gln Val Met Ser Met Glu Leu Gly Ala Asp Asp85 90 95Tyr Met Gln Lys Pro Phe Tyr Thr Asn Val Leu Ile Ala Lys Leu Gln100 105 110Ala Ile Tyr Arg Arg Val Tyr Glu Phe Gly Val Glu Glu Lys Arg Thr115 120 125Leu Ser Trp Gln Asp Ala Thr Val Asp Leu Ser Lys Asp Ser Ile Gln130 135 140Lys Asp Asp Lys Thr Ile Phe Leu Ser Lys Thr Glu Met Ile Ile Leu145 150 155 160Glu Met Leu Ile Asn Lys Arg Asn Gln Ile Val Thr Arg Asp Thr Leu165 170 175Ile Thr Ala Leu Trp Asp Asp Glu Ala Phe Val Ser Asp Asn Thr Leu180 185 190Thr Val Asn Val Asn Arg Leu Arg Lys Lys Leu Ser Glu Ile Asp Met195 200 205Asp Ser Ala Ile Glu Thr Lys Val Gly Lys Gly Tyr Leu Ala His Glu210 215 220
<210>21<211>229<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>21Met Thr His Leu Leu Ile Val Asp Asp Glu Lys Asp Ile Val Asp Ile1 5 10 15Cys Gln Thr Tyr Phe Glu Tyr Glu Gly Tyr Gln Val Thr Thr Thr Thr20 25 30Cys Gly Lys Glu Ala Leu Lys Leu Leu Ser Ser Asp Ile Asp Ile Met35 40 45Ile Leu Asp Ile Met Met Pro Glu Val Ser Gly Tyr Asp Ile Val Lys50 55 60Lys Met Lys Asp Met Gln Leu Asp Ile Pro Phe Ile Tyr Leu Thr Ala65 70 75 80Lys Thr Gln Glu His Asp Thr Ile Tyr Ala Leu Thr Leu Gly Ala Asp85 90 95Asp Tyr Ile Lys Lys Pro Phe Ser Pro Arg Glu Leu Val Leu Arg Thr100 105 110Asn Asn Leu Leu Ala Arg Met Ser Lys Ser Asn His Ser Asn Lys Ile115 120 125Glu Gln Leu Glu Phe Asp Gly Leu Val Leu Lys Asn Leu Ser Lys Thr130 135 140Leu Thr Ile Asn Asn Ile Glu Ile Pro Met Arg Ile Lys Glu Phe Glu145 150 155 160Leu Leu Trp Tyr Leu Ala Ser Arg Glu Gly Glu Val Ile Ser Lys Ser165 170 175Glu Leu Leu Glu Lys Val Trp Gly Tyr Asp Tyr Tyr Glu Asp Ala Asn180 185 190Thr Val Asn Val His Ile His Arg Ile Arg Glu Lys Leu Glu Lys His195 200 205Asp Phe Leu Pro Tyr Thr Ile Thr Thr Val Trp Gly Leu Gly Tyr Lys210 215 220Phe Glu Arg Ser Arg225<210>22<211>200<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>22Met Ile Ser Leu Val Ile Thr Glu Asp Gln Leu Met Leu Arg Lys Ala1 5 10 15Met Val Gln Leu Ile Glu Met His Glu Glu Met Asn Val Ile Lys Asp20 25 30
Phe Glu Asn Gly Val Glu Ala Leu Glu Phe Ile Glu Asn Asn Glu Pro35 40 45Asn Val Ala Ile Leu Asp Ile Glu Ile Pro Gly Leu Thr Gly Leu Glu50 55 60Ile Leu Ser Tyr Val Arg Lys Lys Gln Leu Asn Thr Arg Ile Ile Ile65 70 75 80Val Thr Thr Phe Lys Arg Pro Gly Tyr Phe Glu Lys Ala Val Ala Asn85 90 95Asp Val Asp Ala Tyr Val Leu Lys Glu Arg Ser Ile Asp Asp Leu Ile100 105 110Lys Thr Ile Tyr Lys Val Thr Lys Gly Glu Lys Glu Tyr Ser Pro Ser115 120 125Leu Met Thr Ser Leu Phe Thr Glu Ser Asn Pro Leu Thr His Lys Glu130 135 140Gln Val Val Leu Arg Glu Ile Gly Asn Gly Leu Ser Ser Lys Glu Ile145 150 155 160Ala Ser Lys Leu Tyr Leu Ser Asn Gly Thr Val Arg Asn Tyr Thr Ser165 170 175Thr Ile Ile Asp Lys Met Asn Ser Asp His Arg Phe Glu Ala Trp Lys180 185 190Lys Ala Asn Glu Lys Gly Trp Ile195 200<210>23<211>219<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>23Met Thr Asn Ile Leu Ile Val Glu Asp Glu Gln Asn Leu Ala Arg Phe1 5 10 15Ile Glu Leu Glu Leu Thr His Glu Asn Tyr Thr Val Asp Ile Glu Asn20 25 30Asp Gly Lys Val Gly Leu Asp Lys Ala Leu Ser Lys Pro Tyr Asp Leu35 40 45Tyr Ile Leu Asp Leu Met Leu Pro Asn Ile Asn Gly Leu Glu Ile Cys50 55 60Arg Gln Ile Arg Gln Lys Thr Thr Thr Pro Ile Ile Ile Ile Thr Ala65 70 75 80Lys Ser Glu Thr Tyr Asp Lys Val Ala Gly Leu Asp Tyr Gly Ala Asp85 90 95Asp Tyr Ile Val Lys Pro Phe Asp Ile Glu Glu Leu Leu Ala Arg Ile100 105 110Arg Ala Val Leu Arg Arg Gln Pro Asp Lys Asp Val Leu Asp Ile Asn115 120 125Gly Ile Ile Ile Asp Lys Asp Ala Phe Lys Val Thr Val Asn Gly His
130 135 140Gln Leu Glu Leu Thr Lys Thr Glu Tyr Asp Leu Leu Tyr Val Leu Ala145 150 155 160Glu Asn Arg Asn His Val Met Gln Arg Glu Gln Ile Leu Asp His Val165 170 175Trp Gly Tyr Asn Ser Glu Val Glu Thr Asn Val Val Asp Val Tyr Ile180 185 190Arg Tyr Leu Arg Asn Lys Leu Lys Pro Phe Asn Lys Glu Lys Ser Ile195 200 205Glu Thr Val Arg Gly Val Gly Tyr Val Ile Arg210 215<210>24<211>241<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>24Met Thr Asn Glu Ile Leu Ile Val Asp Asp Glu Asp Arg Ile Arg Arg1 5 10 15Leu Leu Lys Leu Tyr Leu Glu Arg Glu Ser Phe Glu Ile His Glu Ala20 25 30Arg Asp Gly Lys Glu Ala Tyr Glu Leu Ala Leu Glu Asn Asn Tyr Ala35 40 45Cys Ile Leu Leu Asp Leu Met Leu Pro Glu Met Asp Gly Ile Glu Val50 55 60Ala Ser Lys Leu Arg Glu His Lys Asp Thr Pro Ile Ile Met Leu Thr65 70 75 80Ala Lys Gly Glu Glu Thr Asn Arg Val Glu Gly Phe Glu Ser Gly Ala85 90 95Asp Asp Tyr Ile Val Lys Pro Phe Ser Pro Arg Glu Val Val Leu Arg100 105 110Val Lys Ala Leu Leu Arg Arg Thr Gln Thr Ala Asn Thr Glu Gln Ser115 120 125Glu Pro His Ala Arg Asp Ile Ile Glu Phe Ser His Leu Val Ile Asp130 135 140Asn Asp Ala His Arg Val Leu Ala Asp Asp Gln Gln Val Asn Leu Thr145 150 155 160Pro Lys Glu Tyr Glu Leu Leu Ile Tyr Leu Ala Lys Thr Pro Asn Lys165 170 175Val Phe Asp Arg Glu Gln Leu Leu Lys Glu Val Trp His Tyr Glu Phe180 185 190Tyr Gly Asp Leu Arg Thr Val Asp Thr His Val Lys Arg Leu Arg Glu195 200 205Lys Leu Asn Arg Val Ser Ser Asp Ala Ala Gln Met Ile Gln Thr Val210 215 220
Trp Gly Val Gly Tyr Lys Phe Glu Val Lys Pro Ser Asp Glu Pro Thr225 230 235 240Lys<210>25<211>236<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>25Met Ser Gln Lys Ile Leu Val Val Asp Asp Glu Gln Ser Ile Val Thr1 5 10 15Leu Leu Lys Tyr Asn Leu Glu Thr Ala Gly Tyr Ile Val Glu Val Ala20 25 30Tyr Asp Gly Glu Glu Ala Leu Lys Lys Val Glu Thr Glu Gln Pro Glu35 40 45Leu Ile Val Leu Asp Val Met Leu Pro Lys Lys Asp Gly Ile Glu Val50 55 60Cys Lys Thr Ile Arg Ser Asp Lys Asn Leu Val Pro Ile Leu Met Leu65 70 75 80Thr Ala Lys Asp Asp Glu Phe Asp Arg Val Leu Gly Leu Glu Leu Gly85 90 95Ala Asp Asp Tyr Met Thr Lys Pro Phe Ser Pro Arg Glu Val Val Ala100 105 110Arg Val Lys Ala Ile Leu Arg Arg Ser Gln Phe Val Asn Glu Ile Glu115 120 125Lys Glu Asp Val Asp Asp Glu Asp Ile Ile Ile Gly Ser Ile Arg Ile130 135 140Arg Pro Glu Phe Phe Glu Val Tyr Lys Glu Asp Glu Leu Leu Glu Leu145 150 155 160Thr Pro Lys Glu Phe Glu Leu Leu Leu Tyr Leu Ile Glu Arg Gln Gly165 170 175Arg Val Ile Thr Arg Glu His Met Leu Asn Ser Val Trp Asn Tyr Glu180 185 190Phe Ala Gly Asp Ser Arg Ile Val Asp Val His Ile Ser His Leu Arg195 200 205Asp Lys Leu Glu Glu Asn Pro Lys Gln Pro Lys Leu Ile Lys Thr Val210 215 220Arg Gly Leu Gly Tyr Lys Leu Glu Arg Pro Lys Ala225 230 235<210>26<211>207<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>26Met His Lys Val Ile Leu Val Asp Asp His Tyr Ile Val Arg Gln Gly
1 5 10 15Leu Glu Phe Leu Leu Ser Thr Val Asp Asp Leu Ser Val Leu Lys Gly20 25 30Phe Lys Asp Gly Ala Thr Phe Leu Ser Tyr Leu Lys Asn Asn Glu Arg35 40 45Pro Asp Ile Val Leu Leu Asp Leu Val Met Pro Glu Met Asn Gly Ile50 55 60Glu Ile Thr Glu Ile Leu Lys Gln Gln Tyr Pro Glu Ile Lys Val Leu65 70 75 80Val Leu Thr Ser Tyr Val Asp Asp Glu His Val Ile Ser Ala Ile Asp85 90 95Lys Gly Ala Asp Gly Tyr Glu Met Lys Asp Val Glu Pro Gln Lys Leu100 105 110Ile Glu Thr Ile His Lys Val Leu Gln Gly Glu Arg Ile Ile His Pro115 120 125Gln Ala Lys Ser Val Ile Lys Ala Val Ser Lys Lys Pro His Tyr Thr130 135 140Asn Lys Leu Ser Lys Arg Glu Lys Glu Val Leu Lys Glu Met Val Lys145 150 155 160Gly Lys Thr Asn Lys Glu Ile Ala Arg Ala Leu Phe Val Ser Glu Lys165 170 175Thr Val Lys Thr His Val Ser His Ile Phe Ser Lys Leu Glu Val Ser180 185 190Asp Arg Thr Gln Ala Ala Ile Tyr Ala Met Glu Asn Lys Leu Ile195 200 205<210>27<211>209<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>27Met Ala Ile Lys Val Leu Phe Val Asp Asp His Glu Met Val Arg Ile1 5 10 15Gly Ile Ser Ser Tyr Leu Ser Thr Gln Glu Asp Ile Glu Val Val Gly20 25 30Glu Gly Ala Ser Gly Lys Asp Ala Ile Thr Lys Ala His Glu Leu Lys35 40 45Pro Asp Leu Ile Leu Met Asp Leu Leu Met Asp Asp Met Asp Gly Val50 55 60Glu Ala Thr Thr Glu Ile Lys Lys Asp Leu Pro Gln Ile Lys Val Val65 70 75 80Met Leu Thr Ser Phe Ile Glu Asp Lys Glu Val Tyr Arg Ala Leu Asp85 90 95Ser Gly Val Asp Ser Tyr Ile Leu Lys Thr Thr Ser Ala Ser Asp Ile100 105 110
Ala Asp Ala Val Arg Lys Thr Tyr Glu Gly Glu Ser Val Phe Glu Pro115 120 125Glu Val Leu Val Lys Met Arg Asn Arg Met Lys Lys Arg Ala Glu Leu130 135 140Tyr Glu Met Leu Thr Glu Arg Glu Met Glu Ile Leu Leu Leu Ile Ala145 150 155 160Lys Gly Tyr Ser Asn Gln Glu Ile Ala Ser Ala Ser His Ile Thr Ile165 170 175Lys Thr Val Lys Thr His Val Ser Asn Ile Leu Ser Lys Leu Glu Val180 185 190Gln Asp Arg Thr Gln Ala Val Ile Tyr Ala Phe Gln His Asn Leu Ile195 200 205Gln<210>28<211>238<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>28Met Lys Ile Phe Val Cys Glu Asp Asp Gln Arg Gln Arg Glu His Met1 5 10 15Val Ser Ile Ile Lys Asn Tyr Ile Met Ile Glu Glu Lys Pro Met Glu20 25 30Leu Ala Leu Ala Thr Asn Asp Pro Tyr Glu Val Leu Glu Gln Ser Lys35 40 45Glu Leu Asn Asp Ile Gly Cys Tyr Phe Leu Asp Ile Gln Leu Glu Ala50 55 60Asp Met Asn Gly Ile Lys Leu Ala Ser Glu Ile Arg Lys His Asp Pro65 70 75 80Val Gly Asn Ile Ile Phe Val Thr Ser His Ser Glu Leu Thr Tyr Leu85 90 95Thr Phe Val Tyr Lys Val Ala Ala Met Asp Phe Ile Phe Lys Asp Asp100 105 110Pro Ser Glu Leu Lys Met Arg Ile Ile Asp Cys Leu Glu Thr Ala His115 120 125Thr Arg Leu Lys Leu Leu Ser Lys Glu Ser Asn Val Asp Thr Ile Glu130 135 140Leu Lys Arg Gly Ser Asn Ser Val Tyr Val Gln Tyr Asp Asp Ile Met145 150 155 160Phe Phe Glu Ser Ser Thr Lys Ser His Arg Leu Ile Ala His Leu Asp165 170 175Asn Arg Gln Ile Glu Phe Tyr Gly Asn Leu Lys Glu Leu Ala Gln Leu180 185 190Asp Glu Arg Phe Phe Arg Cys His Asn Ser Phe Val Ile Asn Arg His195 200 205
Asn Ile Glu Ser Ile Asp Ser Lys Glu Arg Ile Val Tyr Phe Lys Asn210 215 220Gly Glu Asn Cys Phe Ala Ser Val Arg Asn Val Lys Lys Ile225 230 235<210>29<211>224<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>29Met Ile Asn Cys Leu Ile Val Asp Asp Asp Lys Lys Leu Leu Gln Tyr1 5 10 15Val Ser Ser His Leu Glu Arg Glu Ser Ile Gln Thr His Thr Phe Thr20 25 30Ser Gly Glu Ala Ser Leu Asp Phe Leu Glu Asn Lys Asn Val Asp Ile35 40 45Ala Ile Val Asp Ile Met Met Ser Gly Met Asp Gly Phe Glu Leu Cys50 55 60Gln Thr Leu Lys Asp Asp Tyr His Ile Pro Val Ile Met Leu Thr Ala65 70 75 80Arg Asp Ala Leu Ser Asp Lys Glu Arg Ala Phe Leu Ser Gly Thr Asp85 90 95Asp Tyr Val Thr Lys Pro Phe Glu Val Lys Glu Leu Leu Phe Arg Ile100 105 110Lys Ala Val Leu Arg Arg Tyr Gln Ile Asn Ala Asp Asn Glu Leu Gln115 120 125Leu Gly Asn Leu Ile Leu Asn Gln Ser Tyr Met Glu Ile Thr Val Gly130 135 140Ser Lys Thr Met Asn Leu Pro Asn Lys Glu Phe Gln Leu Leu Phe Leu145 150 155 160Leu Ala Ser Asn Pro Lys His Ile Phe Thr Arg Asp Asp Ile Ile Gly165 170 175Lys Ile Trp Gly Phe Asp Tyr Glu Gly Asp Asp Arg Thr Val Asp Val180 185 190His Ile Lys Arg Leu Arg Gln Arg Leu Ser Lys Leu Lys Ser Ser Val195 200 205Ser Ile Gln Thr Val Arg Gly Gln Gly Tyr Arg Val Asp Gln Asn Val210 215 220<210>30<211>218<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>30Met Lys Ile Val Ile Ala Asp Asp His Ala Val Val Arg Thr Gly Phe1 5 10 15
Ser Met Ile Leu Asn Tyr Gln Glu Asp Met Glu Val Val Ala Thr Ala20 25 30Ala Asp Gly Val Glu Ala Tyr Gln Lys Val Leu Glu His Arg Pro Asp35 40 45Val Leu Ile Leu Asp Leu Ser Met Pro Pro Gly Glu Ser Gly Leu Ile50 55 60Ala Thr Ser Lys Ile Ser Glu Ser Phe Pro Glu Thr Lys Ile Leu Ile65 70 75 80Leu Thr Met Phe Asp Asp Glu Glu Tyr Leu Phe His Met Leu Lys Ser85 90 95Gly Ala Lys Gly Tyr Ile Leu Lys Asn Ser Pro Asp Glu Gln Leu Ile100 105 110Leu Ala Val Arg Thr Val Tyr Gln Gly Glu Thr Tyr Val Asp Met Lys115 120 125Leu Thr Thr Ser Leu Val Asn Glu Phe Val Asn Gln Ser Gln Thr Asp130 135 140Glu Val Ser Ser Ser Ser Asp Pro Phe Lys Ile Leu Ser Lys Arg Glu145 150 155 160Leu Glu Ile Leu Pro Leu Ile Ala Lys Gly Tyr Gly Asn Lys Asp Ile165 170 175Ala Glu Lys Leu Phe Val Ser Val Lys Thr Val Glu Ala His Lys Thr180 185 190His Ile Met Thr Lys Leu Asn Leu Lys Ser Lys Pro Glu Leu Val Glu195 200 205Tyr Ala Leu Lys Lys Lys Leu Leu Glu Phe210 215<210>3l<211>252<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>31Met Lys Ala Leu Ile Val Asp Asp Glu Pro Leu Ala Arg Asn Glu Leu1 5 10 15Gln Tyr Leu Leu Asn Leu Asn Asp Ala Ile Asp Glu Ile Glu Glu Ala20 25 30Glu Asn Ile Glu Glu Thr Leu Glu Lys Leu Leu Tyr Asn Thr Phe Asp35 40 45Leu Ile Phe Leu Asp Ile Asn Leu Met Asp Glu Ser Gly Ile Asp Leu50 55 60Ala Gln Lys Ile Asn Lys Met Lys Arg Ser Pro His Ile Ile Phe Ala65 70 75 80Thr Ala His Glu Lys Phe Ala Val Lys Ala Phe Glu Leu Asn Ala Thr85 90 95Asp Tyr Ile Leu Lys Pro Phe Glu Lys Glu Arg Ile Asn Gln Ala Val
100 105 110Asn Lys Val Asp Met Ala Lys Asp Lys Ser Lys Asn Lys Asp Lys Thr115 120 125Ile Thr Pro Lys Tyr Ile Asp Tyr Ser Asp Asp Glu Arg Ala Gln Thr130 135 140His Val Leu Pro Ile Glu Val Asp Glu Arg Ile His Ile Leu Asn Phe145 150 155 160Thr Asp Ile Ile Ala Leu Ser Val Asn Asn Gly Ile Thr Thr Ile Asp165 170 175Thr Thr Lys Gln Ser Tyr Glu Thr Thr Glu Thr Leu Asn His Tyr Glu180 185 190Lys Lys Leu Pro Ser Ser Leu Phe Ile Lys Ile His Arg Ala Thr Ile195 200 205Val Asn Lys Glu His Ile Gln Thr Ile Glu His Trp Phe Asn Tyr Thr210 215 220Tyr Gln Leu Thr Leu Thr His Glu Phe Lys Tyr Gln Val Ser Arg Ser225 230 235 240Tyr Met Lys Thr Phe Lys Gln Gln Leu Gly Leu Gln245 250<210>32<211>221<212>PRT<213>表皮葡萄球菌(staphylococcus epidermidis)<400>32Met Lys Ile Phe Ile Val Glu Asp Asp Leu Val Ile Ala Glu Ser Leu1 5 10 15Ala Asn Glu Leu Ser Lys Trp Asn Tyr Glu Val His Val Val Asp Asn20 25 30Phe Glu Lys Ile Met Glu Asp Phe Arg Arg Val Glu Pro Gln Leu Val35 40 45Leu Leu Asp Ile Asn Leu Pro Thr Leu Asn Gly Phe His Trp Cys Gln50 55 60Glu Met Arg Lys Val Ser His Val Pro Ile Met Phe Ile Ser Ser Arg65 70 75 80Thr Asp Asn Met Asp Gln Ile Leu Ala Ile Gln Met Gly Gly Asp Asp85 90 95Phe Ile Glu Lys Pro Phe Asn Leu Ser Leu Thr Val Ala Lys Ile Gln100 105 110Ala Leu Leu Arg Arg Thr Tyr Asp Leu Ser Ile Ala Arg Asp Glu Ile115 120 125Ala Val Lys Gly Cys Lys Leu Ile Val Asp Glu Ala Lys Leu Ser Lys130 135 140Asp Asn Glu His Val Gln Leu Ser Leu Thr Glu Leu Gln Ile Leu Lys145 150 155 160
Leu Leu Phe Gln Asn Glu Asn Lys Tyr Val Ser Arg Thr Ala Leu Ile165 170 175Glu Lys Cys Trp Glu Ser Glu Asn Phe Ile Asp Asp Asn Thr Leu Ala180 185 190Val Asn Met Thr Arg Leu Arg Lys Lys Leu Leu Ser Ile Gly Val Asp195 200 205Asp Leu Ile Glu Thr Lys Lys Asn Val Gly Tyr Arg Val210 215 220<210>33<211>1833<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>33atgaagtggc ttaaacaact acaatccctt cacacgaaac tcgttattgt ttatgtacta 60ctcattatta ttggtatgca aatcatcggt ttgtatttta cgaatagttt agaaaaggaa120ttactcgata acttcaagaa gaacataaca caatatgcga agcaattaga cgtcaatatt180gaaaaggtat ataaagataa agataaaggt tcagtcaacg ctcaaaagga tatccaagac240cttttgaatg aatatgcgaa tcgtcaagaa ataggagaaa tacgctttat tgataaagac300caaatcatca tggcaacaac caagcagtct aaccgtggtc ttatcaatca aaaggttaac360gacggttctg ttcaaaaggc gctctcctta gggcaaacga atgatcatat ggttcttaag420gattacggaa gtggtaaaga gcgtgtttgg gtatataata taccggttaa agtcgataaa480cagacaatcg gtgatatata catagaatcg aaaattaatg atgtatacaa tcagctgaac540aacattaatc agatattcat cgtagggaca gcgatatcac tattcattac agtgatacta600ggattcttca ttgcacgaac gattactaag ccgataaccg atatgcgtaa ccaaaccgtc660gagatgtcta aaggtaacta cacgcaacga gtgaagatat acggtaacga tgaaatcggt720gaactcgcac ttgccttcaa taacttatcg aaacgtgtcc aagaagcaca agcgaataca780gaaagtgaga aacgtcgtct agattccgtt atcacacata tgagcgatgg tattcttgcg840acagatcgcc gtggacgtgt acgtattgca aacgacatgg cgctgaaaat gctcggtctc900gcgaaagaag atgtcatcgg ctactacatg cttggtgtcc ttaacttaga aaatgaattc960tcattagaag aaattcaaga aaatagtgat tccttcttgt tagacattaa cgaagaagaa 1020ggcattattg cacgtgtaaa ctttagtacg attgtacaag aaacaggttt cgtgacaggt 1080tacattgccg tactacatga tgtcacagaa caacaacaag tcgaacgtga acgtcgcgaa 1140ttcgttgcga atgtttcaca cgaattacgt acaccgctga catctatgaa tagttatatc 1200gaagcacttg aagaaggtgc ttggcaagat aaagaattgg caccatcatt cctatctgtc 1260acacgcgaag agactgaacg tatgattcgt ttagtgaatg atttacttca attatctaaa 1320atggataatg aatcagatca aattacgaaa gaaattatcg acttcaatat gtttatcaac 1380aaaattatta accgtcatga aatggcagcg aaagatacga cattcgtgcg cgaaattccg 1440caacaaacca tctttgctga aatcgatcca gacaagatga cacaagtatt tgataatgtc 1500attaccaatg caatgaaata ttcacgtggc gagaaacgtg ttgagtttca tgtgaaacaa 1560aatgcacttt acaatagaat gacgattcgt attaaagata atggtattgg aataccgatt 1620aacaaggtag ataaaatatt tgatagattc tatcgtgtag ataaagcacg tacacgtaag 1680atgggtggta caggacttgg tctagctatt tccaaagaga ttgtcgaagc acataacggt 1740cgaatttggg ctaacagtgt agaaggacaa ggtacgtcaa tctttattac gcttccttgc 1800gaaatcattg aagacggtga ttgggatgaa taa1833
<210>34<211>1092<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>34atggctagag tagaaagttt aaaaagaaaa atgacacgtc ttatcatagc aattatagtt 60gtcagtgttg gattagtatt tttactacaa attttgctta atgtattaga attttcaaat120tttggaaagt ctttatacac taatttttgg aaacaaccac attacatttt ctcttttact180agcgcatgta tcagtattat gactcctttg ttaatcattt acgtgttcac acaatttttt240tataaaaagt acattacaac tagtattaat tatcttaatg atacgattca aggattaaaa300agtaaccgta ctgatgttga atataagaaa atgagtattt cagaatttaa taaattgatt360caagaagtag aacatataca tacttatctt tctaatagta ttaaagatca attatctatt420caaaataatt ttaaaagaaa actcgaaatt ttagaacatg atataaaaac accgttaact480gtccttcaag gtcatattga attattaaga aagattaatc gttctaatct gtctcaggag540gaaatacatt ttaaaataaa caaggaagca gacattgcgt tacaatctat tgatagaata600aataatcaat tacaaataca tattaaaaac atcaatctat tgccaaacca ttcagataca660attcatgttg ataaggtaat aaaaatcatt aaagagagct ataatcaaca atacatatta720aaggagagag aactgattat tgatgttgat catgatttcc cttatcatac cttaagcatt780aacaatcaag ttttatacca tgtcatcgat aatctaatta ataacgcctt gaaatatgct840gaaaaacaaa tatctataaa gtttgaagtt ataaacgacc atttactaaa cttcaccata900accaatgatg gtagaatatt tactgaagat gaactgaatt ctgccaaaga atgggggaca960aaaggtaaag aatcaaatgg gtctggtatt ggtctgtatt ttgctaatat tgtgcttcaa 1020caatataata gtgaacttat tttagaaaat gagaataagc atgcaaaagt aagtttcaat 1080attcctgtct aa 1092<210>35<211>1539<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>35atgaagacct ataagccgta ccgacatcaa ttaaggcgtt cgctatttgc ctcaacgatt 60ttcccagtat ttatggtgat gattattggt ttaataagct tttatgctat ttatatatgg120gtcgaacatc gcaccattca tcagcatacc tatcaaactc aaaccgaatt acaacgtatc180gacaaacatt ttcatacgtt tgttacgcag caacaaaaac aatggcgtca tgttgattta240tcacatccag ctgatatcac aaagatgaaa cgccaactat taaaacaagt ccatcaacaa300cctgcgatat tgtattacga tttaaaaggt tcttcacaat ctttcacaaa caattatgaa360caattagaca caacaaagat gtatttaata tcaaaatatc gaattgattt taaagacgat420acttatatcc ttaaaatata tatgtcaagc acaccactac ttaaaaacat taagaaaaat480agtggacaat ctgcactcat tgttgattca tatgatactg ttttatatac aaatgacgac540cgattctcta tcggtcaaaa atatcaacca ccacagtttg ggtttatgaa cgagtcttta600aaactcaatt ctcatcatgc gcatcttatt atatataaag atattcatga aaccattgaa660gatggaattg cattactagt tgtcatgggt gttgttctta ttctgcttgt tatttttgga720tatataagcg ctgatagaat ggcaaagcgc caatctgaag atattgaagc gattgtccga780aaaattgatg atgctaaaaa tcgacatctt ggtagttacg aaccgttaaa aaaacatagt840gagttagagg aaataaataa ttatatctat gacttgtttg aatcaaatga gcaattaata900
caatctattg aacagaccga acgtcgttta cgtgatatac aattaaaaga aattgagcga960caatttcaac cccatttctt attcaatacg atgcaaacga tacaatattt aattcctctt 1020tcacccaaag tagcacaaac agtcatacaa caactatcac aaatgctacg ttattctcta 1080cgcacagcat cgcacacagt caaattagca gaagaattaa gctacattga gcagtatgtt 1140gccatacaaa atatccgctt cgatgatatg atacagcttt acatcgatgc tactgagggt 1200gtacaacatc aaacaattgg taagatgatg cttcaaccac tcgtagaaaa tgccatcaag 1260catggtcgtg atagtgaacc tttaaagata acaattcgta tcagacttac gaagcgcaaa 1320ttacatattc tggttcatga taatggcatc ggtatgtctc catcacattt agaacacgtg 1380cgccaatccc ttcatcacga tgtttttgat acgacacacc taggtttaaa tcatttacat 1440aatagagcca tgattcaata tggaacatat gcacgtttgc acattttctc aagaagccag 1500caagggacat taatgtgtta ccaaatacca cttgtctag 1539<210>36<211>1041<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>36atgaataatt ttcgatggtt ttggtttttt ataaaatctc gtattaattg gatattgtgg 60atattatttc tcaacattat tttattaggt gttgcctaca tagattatga gatttcagtt120gaaagtgtat tttatatagt gatattaaac gtaggattga gtatactttt cttattattt180acgtttgtta aagaagtccg tctatctaaa catttttatg aagataaaga aattgaagaa240attaagcata aagatttggc agaaacccca tttcaacaac aagtcataga ttatctgtat300cgtcatatag ccgctcaaaa agagaaagta gtcgaacaac aattacaaat taaaaatcat360gaacaaacaa ttacagagtt tgttcatgat ataaaaacac ctgttacggc tatgaaatta420ttgatagatc aagagaatga tgatcagcgt aagcgagcgt tattatttga atggtctcgt480attaatgaga tgttagataa gcaattatat ttaacaaggc ttgaaacaca tcatcgtgat540atgtattttg attatatttc attaaagaga atggttatag atgaaataca agttactcga600catatcagtc aggcaaaagg gataggtttt gaattagatt ttaaagacga acaaaaggtt660tatacagatg ttaaatggtg ccgtatgatg attaggcaag ttctatctaa ctctttgaaa720tatagtgata attctacaat aaatttaagt ggttataaca tagaaggaca cgttgtttta780aaaattaaag actacggtcg tggaattagt aaaagagatt taccacgtat atttgataga840ggatttactt ctacaacaga ccgcaacgat actgcgtctt ctggtatggg attatacctt900gtacaaagcg tgaaagaaca acttgggatt gaagttaaag ttgattcaat agtggggaaa960ggaacaacgt tttatttcat tttcccacaa caaaatgaaa tcattgagcg catgtctaaa 1020gtgacaagat tgtcatttta a 1041<210>37<211>966<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>37atgtggttta atggtcacat gacactcact ttagccatca ctactatgat aacaagctgt 60ttaacacttt cgatttgtag tatttttatt aatcctttaa ttcagaagat taagcaattc120aatattaaga ctaagcaatt tataaatcac gaaaaattca ttgatgatga aacatttcag180tctcctagag aaattaaaga gttgaatgat tcttttaata aaatggcata tgaaatcaat240aatcaaatga atatgataaa aaacgagcaa caagaaaaaa ctgaaatcat acaaaacctt300
gcacatgatt taaaaacacc tttagctggt attcgatcat attctgaagg tttacgggat360ggcgtcatta gtgatcctca agaagttcat gaagcatatg aaatattaat taaacaagca420aatcgtttat caattttatt tgatgacatt acacatgtga ttaatttaaa tactggacgt480tcttatcctt tggaattaat acagctcgat caattacttg ttaatatatt acaaccatac540gaacaacaca taaaacaaga aaatcgtaca cttgaagtta atttctgcac agatattgat600gcattctacc agtatcgacc tccaatcgaa cgtattttga ccaatttact agataatgca660ttaaaatttt ctaattctgg tagccgtatt gatattatta tttctgagtg taaagaaaac720gacgtcatta gtatttcaat aaaagatgag ggcataggta tcgttccaga acttcaatca780cgtatctttg aaagaacgtt tagagttgaa gattctcgaa atactaagac tggtggttcg840gggttaggat tatacattgc aaatgagtta gcacaacaaa ttgacgcctc tattacagtc900caaagtgatt tagacattgg aaccacgatg acacttacct taaaaaaatt tcaatttaaa960aagtaa 966<210>38<211>732<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>38atggaatcaa gaagttgggg agcgagaata ggggaattaa gtactttaat atatttaata 60tttccgattt tagctatatt tgttgacaaa cgtggtaact tattcactta tttaattgtt120tgtactattt ttataatcag ctatgtgacg atgattttat tttataaaca ccttagtgat180agtattttat attcattact ggttattcat tatttaggaa tcttttattt tgtctatagt240gtcaatccta tgaatagttt gtttttcttc tatagtgcat tcgccttacc ttttattttt300aatgttcgtg ttgtatcaaa agaatttata acctttttaa tagctatgat aagttgctta360atactaactt atatatttaa tccaacattt gtggttccat taagtgcatt ttatttggtt420atattaattg ttgctgtagg taattttaaa aatagagacg aacgaattat gaaagctaaa480cttgaagaaa agaataagta tattaatgta ctaatagctc aacaagagcg taatagaata540agtcaagatc ttcatgatac attaggacat gtatttgcta gtcttacttt gaagtctgaa600ttagctgtta aactcataga tactaatcca aaagaagcaa aaaatgaaat gcaggccata660aatcaacttt caacagaagc cttaaataaa gtcagactca ttattgatga tttgaagata720ccaatctttt ga732<210>39<211>1371<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>39atgattaagc gccaaaaatt aaaatataaa tggatgctta ttacgacgct cattaccttc 60acgacaatat tacttttctg cttaattatt attttctttt taaaagatac tttacgaagt120agtgaaattg acgaagctga aagaagttca aatgatatcg ccaatctgtt ccattctaaa180tctttaagtg atatatctgc attggattta aatgcatcct tagaaaattt tcaagaaata240ttgatttatg atgataaggg taggaagtta attcaaacat caaatgataa tacacttgct300tatgataaca aaattgattt caaacatcct gaacgtatac atattcaaag aagccacggt360attaattact tagtaataac tgaacctata cgttcgaaag atttttctgg atacagtgta420ttagtccatt ctcttcaaaa ttatgataat ctcgttaaat cactttatat agttgcactt480gcttttggat taattgcaac cattattact gccggcgtga gttatatctt ttcttcacaa540
attactaaac cgatagttac aatgtccaat aaaatgaatc aaattagaag agatggtttt600caaaataaac ttgaattaac tacaaattat gaagaaacag ataatttaat tgatactttt660aatgaaatga tgtatcaaat agaagaatct tttaatcagc aacgtcaatt tgtcgaggat720gcttcacacg aattaagaac gccactgcag attattcaag gtcatctaaa tttaatccaa780cgttggggga aaaaagatcc agcagttttg gaagaatctt tgaatatttc aattgaagaa840gtgaatcgaa taacaaaact tgtcgaagaa ctacttttac ttaccaaaga tagagtcaat900cataatgttt tggaatgtga aaatgtagac gtaaatagcg agattcaatc acgtgtgaag960tcactgcaac acctacatcc agattatact tttgaaacac atcttgctac taagcctatc 1020caattaaaaa ttaaccgtca tcagtttgaa caactcttac tcatatttat tgataatgca 1080atgaaatacg acactgaaca taagcacatt aaaattgtta ctcaactaaa aaataaaatg 1140attatgattg atattactga tcatggtatg ggtataccaa aagctgactt agaatttatc 1200tttgatagat tttatcgtgt agataaatca cgtgctcgta gtcaaggagg caatggatta 1260ggactatcaa tagcggaaaa aattgtgcaa cttaacggtg gtatgattca ggtagaaagt 1320gaactacaaa agtacacgac tttcaaaatc agttttccag tactaaacta a1371<210>40<211>1770<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>40gtgatgaacc gactaaatag tgttgttata aaactgtggt taactattat tttaatagta 60gcgacagttt taattttatt aagtgtttca ttaatcactt ttatacaata ttactttacg120caagaaacag aaaatgcaat taaagaagat gccaaaagaa ttagttcact tgttgagaag180tctcataaca aaacattagc tatccaaaat agccaaacac tcattgatgg tgatggaccg240ggtggcctca ttattatgaa agacatgagc gagaaaccag actcatcgta taaagacact300aaaaaacaaa tgtttaatga aataaaaaag agtactaaat ttaaaaaagt gtttaaagaa360ggtgagtatg aaactcaaaa tattacaata aaaaataaag gtaattctca atcttatctt420ttgctaggat acccaatgaa agctcaaaaa ggtgctcaaa gtcattatag tggtgttttt480atatataaag atttgaaatc tatcgaagat acaaataatg ctattacaat tattatatta540ataactgcta ttatatttac tatagcaagc acaatatttg cattcttttt atctaataga600ataacgaaac ccttacgtca attaaaaaca caagcacaaa aagtttctga aggggattat660agtcaaattt caactgtcgc tactaaagat gaaataggtg atttatcacg tgcatttaac720aacatgaacg tagaaattca agaacatatc aaagcaattt cttcatctaa gaatataaga780gatacattat taaactctat ggtagaaggc gttctaggca ttaataatca acgtgaaatc840atattgtcga acaagatggc tgatgatatt atgcgtcaca ttgatgattt ttcaaaagaa900tctattgaac agcaaattga agcaacattt gaatcacaac agaatgagta tttagaatta960gaaattaata caaggtacta tgtatttatc tccagttata tagatagaat tcaaacaaat 1020ggtagaagtg gtattgtcat ggtcatccgt gatatgacaa atgaacataa tcttgatcaa 1080atgaaaaaag attttatagc aaatgtatca catgaattac gtacgccaat ctctttatta 1140cagggttaca ctgagtccat agtagacggt atagttaccg aaccagatga aatacgtgac 1200tcattagcaa tcgttttaga tgaatctaag cgacttaatc gtttagtcaa tgaattacta 1260aatgtagctc gtatggatgc tgaaggatta tcagttgaga aggaattaca acctattcaa 1320caccttcttg ataaaatgga gtctaaatat cgcatgcaaa gtgaagaatt aggtttaaca 1380atgacgtttg attctaataa tgacgaacaa ttatggaact atgatatgga tagaatggac 1440caagtgttaa ctaatttaat tgataacgca acaagatata cacaagctgg tgattctata 1500
aagatttcta ttgatgaaga ttcagatttc aatattttaa caataactga tacaggcact 1560ggtatagcgc cggaacatct gaaacaagta tttgaccgtt tttataaagt ggacgctgct 1620cgaaaaagag gtaagcaagg caccggatta ggacttttca tttgtaaaat gattattgaa 1680gaacacgggg gacgtattga tgttgagagc gaattaggca aaggtacttc atttattatt 1740agactaccta aatcaaaaca aattagttag1770<210>41<211>1698<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>41atgttgaagt tccatttgcg tttattacta ttaattagta ctattacaat cattagtttt 60attggtttag gcgctatcat tcacaacaca atttatcaaa cattaactag taatcaaatt120aaaagcttag atagtgaagc ccgtaattat gttaatctct ttaataataa taaagagaaa180gaaatcacta atatagctca taatgaaaaa aatatcatat taataaaaga aaaagataaa240gataaaatta tttactcaag tggaaatatt aaagatattg accatagaat tgataatgaa300gctaatcctt cgaaattaat caataaaaac acaaaactcg gtatgcgtta cacctataag360aatacaatag atgataaaac aatatacata agtggaatta ataatgaaat tattgattta420caaaaggatt tatggaaata cttgtctatt gttggagtca ttgtattatt tacggtctat480ttagcaagta gaagtatcaa tcgaacatat attagaccta tcaatgaagt aacttatgct540acatcacttc tagcagatgg atattaccat gttcgtgttc cagaaagtaa tgtgaaggaa600actagggcat tatttgtgac tacaaatgac ttagcacgac gattgcaaaa attaaacaat660agtcaaaaaa ttcaatccaa tagattaaaa actaccttag aaaatatacc gagttcagta720ctgatgattg ataaacatgg agaaattgta gttgctaatc atgcttatta tcaggtgttt780aaccctgatc aaatggtaga aaataaaagt tacattggtt tcatagatga tagtattgaa840aaattaatta ttgaaagttt tagaactgaa aaagttatct atgaacaatt agaagttgct900attaataacg tacatactaa atatttcgat gtatcttgta tccccatttt aactaaatct960aaaaaaaatt tacaaggtat ggtggttgtg cttcatgaca ttactaattt gcagaaatta 1020gaaaacctta gaagggaatt tgttgcaaat gtgtcacatg aactaaaaac accgattact 1080tcaatcaaag gttttgcaga aactctgatt gaaggtgcta aaaatgatga acaatcgctt 1140gatatgtttt taaatattat tttaaaagaa tctaatagaa tagagtcatt ggttacagac 1200ttattagatt tatcacatat agaacagcaa aaagaacttg aaataaatta catgaattta 1260tctgaattag ctattaatat aatagataat ttgcaaacac aagcatacaa taagagaatc 1320aaaatacaat ctgaaattga aaaagatgtc atcattgagg cacatgaaaa taaaatagcg 1380caagttatta ctaatttgct atcaaatgct ataaattatt cttcagaaga taataaggta 1440atagtaagag tatatagaaa tgacaataaa gtttatttag agattcaaga ttatggtatt 1500ggtataagtg aaacagatca aaagcgtata tttgaacgtt tctatcgtgt agataaagcg 1560agaagtagag attcaggtgg tacaggactt ggtctgtcta taacaaaaca tattgttgaa 1620gcacataatg gtagaataga cgtgaaaagt gcacctggca aaggttcgat attcaaagtt 1680ctatttaatg ataattaa 1698<210>42<211>1134<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>42
atggagaaac caactagact tgcattactt aaggaaatag ccgaatttct aaatgaagaa 60actgaaatat atagcatgat gcaaggagct ttgcgtttac ttataaatgg gagcgacttt120tcaactggtt ggattttcta tattgatgaa gaggggcacc atgatttagt ttcctctgta180gatttacctg aagctttatc taaaaaccat tgttactatt tgacaaatgg ttcttgttgg240tgtgtgcaag catatcaaaa tcataaatta actaaagctt ctaatataat aaattgttct300agaatcaatt tagctaatca tgcttatgat aaagaaacga atcatattac tcatcatgct360actgttccat tgcagtctgg gagcgaaaga ttcggtttgc ttaacgtagc atctcctaac420aaagaacatt atagtgaaga agacttagaa ctattagaat ctgtagcatt tcaaattggt480tcagctataa agagaattca gctaactaat aaagaaaaag aaaatgccaa aattaatgaa540cgtaatcgac tagctagaga cttacatgat tcagtaaatc aaatgttatt ttctttaaaa600ttaacggctc acgctgcaaa agggataact actgacgagc aagcgcaaag agcttttaag660actattgagg aaacaagtca aaatgcagta aatgaaatga gagcactgat ttggcaacta720aaaccagttg gattagaaca aggcttaatt catgcattac actattatag taaaatgtta780ggattagatt tgaaagttac tattaatgga ctaatagatt tatctaatga tatcgaagaa840aatgtataca gaattattca ggaagcaatg aataatgtta ttaagcacgc taaaactaac900aaagtttatc ttgatttatc acaaaatgtt gattatcttg aggtagatat tagagataac960ggtcaaggtt ttgacacgca atcaattaat acgtttttat ttaatggttt aaaaaacata 1020aagcaacgta cacagtattt gaacggtaaa gttactatag aatcaaaaat aaatcagggc 1080actcggattt atattaaaat tccattgaag ttaaaggagc atccttatgc ataa 1134<210>43<211>1047<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>43atgaatcact acataagagc tataggctca atgcttatat tagtatatag tatgcttatt 60gcttttttat ttattgataa agtgtttgta aatattatct tttttcaggg gatgttttat120acacaaatat ttggaatacc tgtttttcta tttttaaatt tattaattgt tcttttatgt180attatagttg gatctgtttt agcttataaa attaatcaac aaaatgattg gattatttca240caaatagaaa gatcaataga aggacaaaca gtaggtatca atgatcaaaa tatcgaatta300tatacagaaa cgatagatat ttatcataca ctagttccat taaatcaaga attacatcga360cttagaatga agactcaaaa tttaactaat gaaaactaca atattaatga tgtaaaagtc420aaaaagatta tcgaagatga gcgacaacga cttgccaggg aattacatga ttctgttagt480caacaattat ttgctgcgag catgatgcta tcggcgataa aagagtcgaa attagaacca540cctttaaatc aacagatacc aattcttgaa aaaatggttc aagactcaca acttgaaatg600agagctttgt tattacattt aagaccgata ggtttaaaag ataagtcttt aggtgaagga660attaaagatt tagtcatcga tttacaaaag aaagtaccaa tgaaagttgt gcatgaaatt720caagattttg aagtgccaaa aggcattgaa gatcacttgt tcagaattac acaagaagct780atttcaaata cattgagaca ttcaaatggt acaaaagtaa ctgtggaatt atttaatcaa840gaggattatc ttttactaag aattcaagat aatggaaaag ggtttaatgt agatgaaaaa900tttgaacaaa gttatggttt gaaaaatatg cgagaacgag cgttagaaat tggtgcgacg960tttcatattg tatctttacc tgattcaggt acacgaattg aagttaaggc accattgaat 1020aaggaggaga attcaagtgg cgattaa 1047<210>44<211>1290
<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>44atggatgata ttaatctatt tccgtttgca ggcctacaaa tctttttaat gatttgggtt 60actaaagtta tcattaatat gaaatttaat tttagggatt acataatcgt ttttacgatt120gtaatccctt ctgcaataat gtattacttt tggcaaagta aagcattaat agttttggtt180ataataatca ccattttctt ttatacaaaa ataaagcttt attcaatatt agttgtatta240ttcaccacta tgatcttata tataactaat ttcataactg tatacataca tttgactata300aaagattata ttccgtttaa atttgcttta cagttaatac attttacctc ttttgtaatc360ataactctaa ttattgctta tttaactcaa ctattgttca ataaattaaa agtatcctac420ttgtcactca ataaaagata cttattgata ataacaatag tacttttcat atcatttatt480ttactttata tggtgtcaca aactgatatg cgaggaaatg atacccttaa attatatgcc540atcttattgt tgggtattat ggttttttta agtgtagtga tattagtgat gtccaatttt600acactccgtg aaatgaggta taaacgtaat gtaaaagaaa tcgaagcata ttatgagtac660acgttacgta tagaaagcat taacaatgaa atgcgtaagt tccgacatga ttatgtgaat720atcctcacca ctctttcaga ttacattaga gaagatgata tgcctggatt acgtaaatat780tttaatgaaa atatcgttcc aatgaaagat aaattaaaaa ctcgctctat taaaatgaat840ggtattgaaa agttgaaagt gagagaaatt aaagggctga ttactactaa aattattcaa900gctcaagaaa aacgtattcc aattagtatt gaggttcctg atgaaattga tcgtatcgat960atgaatactg ttgagcttag tcgtattatc ggtattatag ttgataatgc tattgaagct 1020tcagaaaatc ttgaggaacc actcatcaat atcgcattca tcgataatga ggaatctgtc 1080acttttatcg ttatgaataa atgtagtaat gatatcccta aaattcatga gttgtttgaa 1140caaggttttt ctactaaagg tgataatcgc ggtttaggtt tatcaacttt aaaagaactg 1200acagactcaa acgagaatgt tttattagat actgtcatcg aaaatggtta ctttgtacaa 1260aaagtagaaa taaataataa ggaatcataa1290<210>45<211>1356<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>45atgtttaaaa cattatattc gagaattgct atatatgcaa ttacagttat tttgtttagt 60gcattaatga gttttctttt tacgaatatt tattatcact ttcatttgaa agcttctaat120gatgcaaaaa ttatgcgcac actcaaagaa gctcgtgaat atgaaagaac acaaaaacct180aaacctctag atacgtattt gaaacattta ggtcaaatga attatcaaat tatgactgtc240aacgaacatg gaaccaaaca tttttatggg gaaacttttc gtaaaaatac tataagtcag300tctgcaatca aaaaagtgct caacggtgaa gattatcatg gtattaaaaa taaaccttat360gcattttttg taacagggtt cttcgataat gaaacggata atacagtagg tattcaattt420aaaacagatg atggggcatt agcagttttt atgcgcccag atatcgggaa aacttttagc480gaatttagaa ttttcttagc tgtgttgata acacttttac ttattatttc aatttcacta540gtcattgcat caacatattc tattattaaa ccagtgaccg cattaaaaaa tgcaacaact600cgaattatga aaggtgattt cagtacgcct atcaaacaaa cacgtcacga tgaaatcgga660acactgcaat cacgtttcaa tacaatgcgt caaaatttag gacaagtaga tcaaatgaga720caacattttg tccaaaatgt atctcatgaa attaaaacac cgcttacaca tttacaacga780ttacttacac aacttgaatt gacgcaaaat gaagaagaga aacaactatg tatcaatgaa840
atgtttgaaa tcactaatca agtaagtgaa ctaactaagg aattgctact tttatctgaa900ttagataatg cttcccacct tacttttaac gataatgttc atttaaatac tttaataaag960gatattatta gacatgaaca atttcgaaca gatgaaaaag atttagtttt gtttactgaa 1020ttagaagatt tgtattttcg aggaaatgaa agactattac atcaagcttt caataacctc 1080atcattaatg caatgaaata tgctcctcaa aatagcatga ttaatatcac tctaactagt 1140acaaatcact tgattatatt taatattgaa aatgatggat cgattgcaga agaagatgcg 1200aaacatatat tcgatcgttt ttataaactg agtgacgaat ctagtagtaa tggtctaggt 1260ctagccatta cccaatcaat cattcatctt catcatggta gcattactct cacttcagat 1320gataaaacac aatttattgt aaaactattt atttag 1356<210>46<211>1044<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>46gtggtaaata tgttggagca aactgattta agtttagagc aattacttaa gaattattat 60gaaaccacga acgagaaaat tgtatttgtt aatagacaag gcaaaattat tgctatgaat120gacgcagcaa aagatatttt aactgaggaa gataattata atgctatgac aaatgcgatt180tgtcatcgat gcgaaggata ctctaatgaa tatgatgtac aatcgtgtaa agattgtttt240ttagagacaa cgcaattaca acattccaat ttccaagtat ttatgaagac aaaagataat300gaaattaagc cttttacagc tatgtatcaa aatattgatg aacaaagagg tattagtgca360tttaccttac agaatgtggc gcctcagatt gaaaggcaag aaaaaatgta tcaacaaaaa420atgttacatc gttcaattca agcacaagaa aatgaacgaa agcgtatttc tagagaatta480catgatagtg taatacagga tatgctcaat atagatgttg aactaaggct tttgaagtat540aagcacaggg ataaggtgtt agctgaaaca tctcaacgta tagaaggact attatcacag600cttattgatg atattagaaa tatgtctgtt gaattaagac cttcttctct cgacgattta660ggtattgaag cagcttttaa atcatatttt aaacagtttg aagaaaatta tggtatgcat720attaaatatg attcgaatat taaaggcatg cgttttgata atgaaattga aacagttgtg780tatcgtgtag ttcaagaggg tgtatttaat gctctaaaat atgctgaggt taatgaaatt840gaggtaagta cgcatagtga tggcaagcag cttgtagcag aggttgtgga tcgaggtaaa900gggtttagtt tagatcatca ccctaaaggc tctggacttg gattgtacgg aatgagagaa960cgtgcagaat tagttaacgg tcatgttaat atagagacac atattaatag aggtactata 1020attacattag atataccgat ttaa 1044<210>47<211>1791<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>47atgaggattg acgttatgct taatttattt attttattac ttgaacgcgt gggattaatt 60attttactcg catatatact catgaatatt aatcatttta aaacaatgat gagcgaacgt120gacaaatggc gttcaaaatt ccaactaatc attatttttg gtatattctc tatgatttca180aattttacag gaattgaaat agaaaatggc catatcgtat ctagtgatat ttattaccac240ttaagcaaag acgcctcaat ggccaatacc cgtgtattaa caatcggtgt ttctggtcta300ataggtggac cgtgggtagc tataattgta ggcattattt caggattatg tcgtttatat360attggcgggg ctgacgcata tacctatctt atttcatcca tagttatcgc tatcatatct420
ggttactttg gtcatcaaac aataaaacaa aatacatatc cgtccattaa aaaaggtgct480attatcggtg caataactga aattattcaa atgggctgta tattattatt tacaaataac540ctacatcacg caattacatt agtcagtttt atagcactac cgatgattat cattaatagt600ttaggtacag ccatattttt aaccatcatt ttgtcgacca tcaagcaaga agaacaaatg660cgtgcagtcc agacgcatga tgtattacaa ctcgctaacg aaacactacc ttactttcga720tctggactta atgaaaaatc agcccaacaa gctgctgaaa ttattttaaa attaatgcaa780gtgtcggcgg tcgctattac aaataaaaaa gatattttaa cacatattgg tgcaggcagt840gaccatcacg tagcacgtaa agagataatt actgatttat ctaaagaagt gattcaatct900ggaaaattaa aagtcgctca tacgagagaa ggaattggat gtcaccatcc taattgccct960cttgaaggag ccattgtcgt ccctctttat atacataatg aagtcgcagg gactttaaaa 1020ttttatttca cagataacaa tattatttca acttcagacc aacagctcgc aaaaggtctc 1080gccaatatat tttcctcaca gctagaacta ggtcaagctg aaatgcaagg acaattattg 1140aaagacgctg aaattaaatc attacaagca caagtcaatc cacatttctt ttttaatgct 1200attaacacta tatcagcact agtaagaatt gatagcgaaa aagcacgacg tttactgatt 1260caactcagtc agttcttccg ctctaatctt aatggtgcac gtaataatac gattacttta 1320caaaaggaat tacaacaagt agcagcatat ttatccttag agcaagctcg ctatccaaat 1380agatttaaca tacactatcg aattgatgat cagtgtcaag atgcgctcat cccccctttt 1440ataattcaaa tattagtgga aaattctatt aaacatgcat ttaaaaatcg taaaaagaat 1500aatcatattg atgtggatgt tagcatgaag caagactgct taagtatatc tgttcaagat 1560aatggtcaag gcataccagc tgatcaatta gatactattg gatatacgac agtaacgtct 1620accactggta ctggtaatgc cttagtcaat cttaataaaa gacttactgg actatttgga 1680acaacatcgg cactgaacat tcaatcttct caatcaggca cgactgtaag ttgtttaatt 1740ccatataaat cttctaagga ggaacacttt aatgaaagcg ttaatcgttg a1791<210>48<211>897<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>48atgaagtttg catatattca atcgattcgt aatgagattt caattatttt aataattcta 60ttattttttg cacttatatt ttatgtgttt tctttacctt ttgatgcata cgtactagca120atcagtataa tattactatt gatgtgtgta cgttggtgga taaagtattt aagttttaaa180aagaatgaac atcttaaaga taaagtagca tatttagaac atgagttagc acatgttaag240aatcagcaaa ttgaatatcg taacgatgtt gaaagttatt ttttaacatg ggtacatcaa300attaaaacac ctatcactgc ctcacaatta cttttggaga gaaacgagga gaatgtagtt360aatcgtgttc gacaagaaat tgtgcacatt gataattata caagtctcgc attaagttat420ttaaaattat taaatgaaga gtcagatatg acaattacca aagtgacagt tgatgatttg480attcgcccgt tgattttaaa atatagaatt cagtttattg aacaaaagac gcaaatccat540tatgaaaaaa gtgaggacat tattttaacc gatgcacaat gggcttctat aatgatagag600caacttttaa ataatgcttt aaaatatgct aaaggtaaag atatttggat agattttgat660gttgccaatc aaactctaca gattaaagat aatggtattg ggattagtaa agcagatatt720cctaaaattt ttgataaagg atactcagga tttaacggta gattgaatga acaatcaact780ggtataggtc tatttatagt gcaacacatt gcaaatcatt taaatataca agtaactgta840caatcagagt tgaatcatgg gacagtattt tttatacatt ttactaaaga aaaataa 897<210>49
<211>702<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>49atggctagaa aagttgttgt agttgacgat gaaaaaccaa ttgctgatat tttagaattt 60aatttaaaaa aagaaggtta cgacgtatat tgcgcttatg atggtaatga cgcagtagat120ttaatctatg aagaagaacc agatatcgtc ttacttgata tcatgttacc tggtagagat180ggtatggaag tatgtcgtga agtgcgtaaa aagtatgaaa tgccaattat catgttaaca240gcgaaagatt ctgaaattga taaagtatta ggccttgaat taggtgcaga tgattacgta300actaaaccat ttagtactcg tgaactcatc gcacgtgtga aagcgaactt acgccgtcat360tattcacaac cagctcaaga agtaagtggt gcgacaaatg aaattacaat taaagatatt420gtgatttatc cagatgcata ttcaattaaa aaacgtggag aagacattga attaacgcat480cgtgaattcg agctattcca ttatctttct aaacatatgg gtcaagtcat gacacgtgaa540cacttactac aaacagtgtg gggttacgat tatttcggtg atgttcgtac tgtggacgta600acaattcgcc gtttaagaga aaaaattgaa gatgatccat ctcatccaga atacattgtg660acacgtagag gcgttggata cttcctccaa caacatgatt ag 702<210>50<211>669<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>50gtgatgtata acataatgat tgtcgacgat gaagaaaaaa ttgtgtcatt tatagaagaa 60agtttatact tagaaggttt taacactatt gtcgcttata atggacgtga cgttttaaaa120caaatcgatg acaaaattga tttaatcatt ttagatatta aaatgccgta tatagatggg180tttgaagttg ctcaatcact taaagagagt aatataccta ttatttttct tactgctaaa240gataatcttg atactagact gaaatgtttc tcgcttggtg caaaggatta cttacctaag300cctttctata tggaagaact tataattcga attaaaaatg tacttaatca aagtcattct360caaagtttga ctcccaacat aaaaaaaact aaagaattaa tcatttatta cgatacatat420agaattgacg taaataacat gacgatagat attacaaaaa aagaatttga aattataaaa480ttactatctt taaatcctca atattatttt agtaaagaac agatatatga ttcgattaat540ttaaataaga caggcaatac tcaagttgtt gctgaacata tacgtaaaat aagaaaaaag600ttaagttgtt atagtcgttt tgaatacatc gatacaaagt ggggagttgg atataaatgg660ctagagtag669<210>51<211>792<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>51gtgttaccaa ataccacttg tctaggagga ataaagatgt ttaaagtagt tatttgtgat 60gatgaacgga ttatacgaca aggtttaaaa caaatgattc catggaagga atatcatttt120acaactattt acactgccac agacggcgta gaagcattgt ctttaattcg ccaacatcaa180cctgaactcg tcattactga tatacgaatg cctcgaaaaa atggtgttga cctactagat240gacatcaaag accttgattg ccaagttatc attttatcta gttatgacga cttcgaatat300atgaaagccg gcattcaaca ccatgttctt gattatttac taaagccagt agaccacact360
caattagagc atattctaga catattagtt caaaggttat tagaacgtcc acattctacc420aatgatgacg cggcatatta tactgccttt caaccattat taaaaattga ttacgatgac480tattatgtca atcaaatttt gtctcaaatc aagcaacatt atcacaagaa agtgactgtt540cttgacttaa ttaatcctat tgttgtaagt gagtcatacg ccatgaggac gtttaaagaa600catgtaggca ttacgatagt tgattattta aatcgttatc gtattttaaa atcattacat660cttttagacc agcactacaa gcattatgaa attgctgaaa aagtaggttt ttctgagtat720aagatgtttt gctatcattt taaaaaatat ttacatatgt caccaagtga ttataataag780ctatcaaaat ag792<210>52<211>675<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>52atgcaaatat tattagtcga agacgataat acgctttttc aagaattaaa aaaggaatta 60gaacaatggg attttaatgt cgttggtgtc gaagatttta gtcatgtgat ggagacattt120gaaacattta atccagaaat agttattttg gatgttcaat taccgaaata cgatgggttt180tattggtgtc gaaaaatgag acaacagtcg aacgttccta ttttattttt atcatcaaga240gataatccaa tggatcaagt gatgagtatg gaacttggtg cagatgatta tatgcaaaaa300ccattttata caaacgtctt aattgctaag ctacaagcta tttatagacg cgtttatgaa360tttggagttg aagaaaagag aacgttaagt tggcaagacg ctactgtgga tttatcaaaa420gatagtattc aaaaagatga taaaactatc tttttgtcta aaacagagat gattatttta480gagatgttaa tcaataaacg taatcaaatc gtgacacgag acactctcat tactgctttg540tgggatgatg aagcttttgt tagtgataat actttaacag ttaatgttaa tagattaaga600aaaaaattat cagaaattga catggatagt gcaattgaaa ccaaagttgg taaaggatac660ttagctcatg aataa 675<210>53<211>702<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>53gtgaagataa gcatgaccca cttactaatc gtggatgatg aaaaagatat tgtagacatt 60tgtcaaacgt atttcgaata tgaaggatat caggttacga caacaacatg tggtaaagaa120gcactaaaat tattatcttc agatatagat attatgattt tagacattat gatgccggaa180gttagcggat acgatattgt caaaaaaatg aaagatatgc aacttgatat acctttcata240tatctaacag caaaaactca agaacatgac acgatatacg cactgacgtt aggtgctgat300gactatatta aaaaaccctt tagcccaaga gaactagtat tacgtactaa taaccttctc360gctagaatga gtaaatcaaa tcattctaat aaaattgagc aattagaatt tgatggttta420gttttaaaaa atttaagcaa gacacttacc attaacaata tagagatacc tatgcgcatt480aaagaatttg aattgctatg gtatcttgct tctagagaag gtgaagttat ttctaaatcc540gaacttctag aaaaagtatg gggatatgac tattacgaag atgctaacac tgtcaatgtc600cacatacata gaattcgcga gaaattagaa aaacatgatt ttttacccta cactattaca660actgtgtggg gccttggtta taagtttgaa aggagccgat aa 702<210>54<211>603
<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>54atgatatcgc ttgttataac tgaggatcaa ttgatgttaa gaaaagctat ggtacaactg 60atagaaatgc atgaagaaat gaatgtaata aaagattttg aaaatggtgt agaggcactc120gaatttattg aaaataatga acccaatgta gccattttag atattgaaat tccgggttta180acaggcttgg aaatcttatc ttatgtaaga aaaaaacaac tcaatacaag aataatcata240gtgactacat ttaaacgtcc aggttatttt gaaaaagccg tagcaaatga tgttgatgct300tatgtactaa aagagcgttc aattgatgat ttaattaaaa ctatttataa agtgacaaag360ggtgagaagg aatatagccc ttcgctcatg acatcattgt ttactgaatc gaatccatta420actcataaag aacaagttgt tttaagagaa ataggcaatg ggttaagtag caaagagatt480gctagcaaat tgtatttatc gaacgggact gtgcgtaact atacgagtac tattatagac540aaaatgaatt cagatcatcg ttttgaggca tggaaaaaag caaatgaaaa ggggtggatt600taa 603<210>55<211>660<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>55atgacaaata ttttaattgt agaagatgag caaaatcttg ctagatttat agagcttgag 60ttaactcatg aaaattatac tgttgatata gagaatgatg gaaaggtggg tttggataaa120gcattatcta agccctatga tttatatata ttagacttaa tgcttccaaa cataaatggt180ctagaaattt gtagacaaat tcgtcaaaaa acaactactc caattatcat cattactgcg240aaaagcgaga catatgataa agtagctgga ttggactatg gggcagatga ctacattgta300aaaccctttg atatagaaga attgcttgca agaataagag cggtattgcg cagacagcca360gataaagatg ttttagatat caatggtatt atcattgata aagatgcctt taaagttact420gttaatggcc atcaattaga attaactaaa acagaatacg atttactata tgttttagct480gaaaatcgta accacgtcat gcaacgtgaa caaattctcg atcacgtatg ggggtataat540agtgaagtag aaacgaatgt cgttgatgtt tatattcgtt atttacgtaa taaactcaaa600ccttttaata aagaaaaatc catagaaaca gtacgtggcg tagggtatgt gattcgatga660<210>56<211>726<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>56atgactaacg aaattttaat cgttgatgat gaagatagaa ttagaagatt acttaaatta 60tatttagaaa gagaatcttt tgaaattcat gaagcaagag atggtaaaga agcctacgag120cttgctcttg aaaacaacta tgcgtgcatc ctcttagatt taatgcttcc tgaaatggat180ggaattgaag ttgcctctaa acttagagaa cataaagata cacctatcat catgcttact240gcaaaaggtg aagaaacaaa tcgtgttgaa ggatttgaat cgggagcaga tgattatatt300gttaagcctt tctcacctag agaagtagta ttgagagtaa aggcgttatt aagacgtaca360caaactgcaa acactgagca aagcgaaccg catgcacgcg atattattga atttagtcat420ctagtgattg ataatgacgc tcatcgagtc cttgcggatg atcaacaagt gaatctaaca480cctaaagagt atgaattact catctattta gctaaaactc caaataaagt atttgatcga540
gaacagttac ttaaagaggt ttggcattac gaattttatg gggacttgcg tacagtagac600actcacgtca aaagacttag agaaaagtta aatcgagtat caagtgatgc tgcacaaatg660atacaaactg tgtggggtgt cggttataaa tttgaggtaa aacctagtga tgaaccgact720aaatag 726<210>57<211>738<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>57atgtataaaa agattaggag gcacactatg tcacaaaaaa tattagtggt tgatgatgag 60caatccatcg tgaccttact aaaatataat ttagaaacag cagggtatat agtcgaagtc120gcttatgatg gtgaagaggc attgaaaaaa gttgaaacag aacaaccgga actaattgta180ttagacgtta tgttgccaaa aaaagatggt atagaagtat gtaaaacaat caggtctgat240aaaaatttag taccgatttt aatgttgaca gcaaaggacg atgaatttga tcgagtttta300gggcttgaac taggtgctga tgactatatg acaaaaccat tttcacctag agaggtagtt360gccagagtta aagcaatatt aagacgttct caatttgtaa atgaaattga gaaggaagat420gttgatgatg aagatattat cattggttca attagaattc gacctgaatt ctttgaagtc480tataaagaag atgaattatt agaattgacg ccaaaagaat ttgaattgtt attatattta540attgaaagac aaggaagagt tattactcga gaacatatgt tgaattcagt gtggaattat600gaattcgctg gagattctcg aattgttgat gtacatataa gccacttaag agataaattg660gaagaaaatc ctaagcaacc taaattgata aagactgtac gtggtttagg ttataaactg720gagagaccta aagcttaa 738<210>58<211>624<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>58atgcataaag tcattctcgt cgatgatcac tatattgtaa ggcaaggttt agagttttta 60ttatcaactg tagatgattt atcagtttta aagggtttta aagatggcgc aacattttta120tcttatttaa aaaacaatga aagaccggac atcgtgcttt tagacttagt aatgccagag180atgaatggta tagaaattac tgaaatactc aaacaacaat atcctgaaat caaagtatta240gtacttacga gctatgtaga tgatgaacat gttatttcag ctattgataa aggagctgat300gggtatgaaa tgaaagatgt tgaacctcaa aagttaattg agacaattca taaagttttg360caaggtgaac gtattataca tcctcaagct aaaagcgtga ttaaggccgt atcaaaaaaa420ccgcattata cgaataagtt atctaaacgt gaaaaagaag tgcttaagga aatggttaaa480ggtaaaacaa ataaagaaat tgcaagagca ttgtttgtat cagaaaagac agtaaaaaca540catgtgagtc atatttttag caaactagaa gtctccgata gaactcaagc cgctatttat600gcaatggaaa ataaattgat ttaa 624<210>59<211>630<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>59gtggcgatta aagttttatt tgttgatgat catgaaatgg taagaatcgg tatttcaagc 60
tatctttcaa ctcaagaaga tatagaagta gtaggtgagg gtgcttctgg taaagatgct120atcactaaag cgcatgagct aaaaccagat ttaatattaa tggatttact tatggacgat180atggatggtg tagaagcaac tactgaaata aaaaaagatt tacctcaaat taaagtagtc240atgttaacaa gctttataga ggataaagaa gtttatcgtg cacttgattc tggagtagat300agttatattt taaagacaac aagtgcaagt gatatagctg acgctgtgcg taaaacgtat360gaaggtgaat cagtatttga accagaagtg ttagtaaaaa tgcgcaatcg tatgaaaaaa420cgtgccgagc tttatgaaat gttgacagaa agagaaatgg agatcctatt acttatagct480aaaggatact ctaaccaaga gattgcaagc gcctctcata tcaccatcaa aacagtaaaa540actcatgtaa gtaacatact aagtaaatta gaagtacaag atcgaacaca agcagtaata600tatgcgttcc agcataattt aattcaataa 630<210>60<211>717<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>60atgaaaattt ttgtttgtga agatgaccaa agacaaagag aacatatggt atcaatcatt 60aaaaactaca taatgattga agaaaagcca atggagttag ctttagcaac aaatgatcct120tatgaggtct tagagcaatc aaaagaactt aatgacattg gttgttactt ccttgatatt180caattagaag ctgatatgaa cggtattaaa ttagccagtg aaattcgtaa acatgatcct240gttggtaata ttatatttgt aaccagtcac agtgagctga cttatttgac gtttgtttat300aaagtggctg ctatggattt tatttttaaa gatgatccat ctgaattaaa aatgagaatc360atagattgtc ttgaaacagc acatacacga ctcaaattat tatcaaaaga aagtaatgta420gatacgattg agttaaagcg gggaagtaat tcagtatacg ttcaatatga tgatattatg480ttttttgaat catctacgaa atctcataga ctcattgcac atcttgataa tcgacaaatt540gaattttatg gaaatttaaa ggaattagca cagcttgatg aacgtttctt tagatgtcat600aacagttttg taataaacag gcataatatt gaatctattg actcaaaaga acgtattgtt660tactttaaga atggcgaaaa ttgtttcgct tcagtacgta atgttaaaaa aatataa 717<210>61<211>675<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>61atgattaatt gcttaatcgt agacgatgat aaaaagttat tgcaatatgt ttcaagtcat 60ttagaaagag aaagtattca aacacatact ttcacaagtg gagaagcatc actagatttt120cttgaaaata aaaatgttga tattgcgata gtagatatta tgatgagtgg aatggatggt180tttgagcttt gtcagacttt gaaagatgat tatcatattc ctgtcataat gttgacagct240agagatgcat taagtgataa agaacgtgca tttctaagtg ggactgacga ttatgtcact300aaaccttttg aggttaaaga attattattt agaattaaag ctgtcttaag acgatatcaa360attaatgctg ataacgagtt acaacttggc aacttaatat taaatcagtc ttatatggaa420attactgtgg gttcaaaaac gatgaatctt ccaaacaaag aatttcagtt gttattttta480ttagcctcta atcctaaaca tattttcact cgagatgata ttattggaaa aatttggggc540ttcgattatg aaggagatga tcgtactgtt gatgttcata ttaaaagatt acgtcaacgt600ttatctaaat tgaaatcatc agtatcaatt caaactgtaa gaggtcaagg atatagggtg660gaccaaaatg tttaa 675
<210>62<211>657<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>62gtgaaaatag ttatagcgga tgaccatgca gttgttagga caggattttc aatgatatta 60aattatcaag aagatatgga agttgttgca actgcagctg acggggttga agcttatcaa120aaagtgttag aacatcgacc agatgtttta attttagatt tgagcatgcc accaggagag180tctggcttaa tcgcaaccag taaaatttct gaaagttttc ctgaaactaa aattttaata240cttacgatgt ttgatgacga agaatattta tttcatatgt taaaaagtgg tgctaaagga300tacattttaa agaattcacc tgatgagcaa ttaatattgg ccgtacgtac agtatatcaa360ggtgaaactt atgttgatat gaaattgacg acgtctttag tcaatgagtt tgtcaatcaa420tctcaaacgg atgaagtgtc atcctcttca gatccattta aaattttatc gaaacgagag480ttagaaatat tacctcttat agctaaaggc tatggcaata aagatattgc agaaaagttg540tttgtatcag tgaaaacggt agaggcacat aaaacgcata ttatgacgaa actaaattta600aagagtaaac ctgaattagt tgaatatgcc ttaaagaaaa aattattaga attttaa 657<210>63<211>759<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>63atgaaagcgt taatcgttga tgatgaacca cttgcaagaa atgaattaca atatctttta 60aatcttaatg atgctataga tgaaattgaa gaagcagaaa acatagaaga aacattggag120aagttactct acaacacatt tgacttaata ttcttagata taaatttaat ggatgaaagt180ggtattgatt tagctcaaaa aattaataaa atgaagcgat caccacatat tatctttgca240accgctcacg agaaatttgc agtcaaagcc tttgaattaa atgcaaccga ttatatatta300aaaccttttg aaaaagaacg tattaatcaa gctgtaaata aggttgacat ggctaaagat360aaatcaaaaa acaaagataa aactatcaca cctaaatata ttgattatag tgatgatgag420cgcgctcaaa cacatgtact cccaattgaa gtggatgaac gtattcacat cttaaatttc480acagacatta tcgcattatc tgttaataat gggattacaa cgatagatac aacaaaacaa540agttatgaaa cgaccgaaac acttaatcat tacgagaaaa aactaccttc ctctctattt600attaaaatac atcgcgctac tatcgttaat aaagaacata tccaaacaat agagcattgg660tttaattata cgtatcagct gacgttaaca catgaattta aatatcaagt tagtcgttct720tatatgaaga cttttaaaca acaacttggt cttcaataa 759<210>64<211>666<212>DNA<213>表皮葡萄球菌(staphylococcus epidermidis)<400>64atgaagattt tcattgtaga agacgattta gtgattgctg aaagtttagc taatgagcta 60tctaaatgga attatgaggt gcatgtggtt gataattttg aaaagataat ggaagatttt120cgccgagtag aaccacaact cgtattatta gatatcaatc taccaacact taatggcttc180cattggtgcc aagaaatgcg aaaagtatct catgtgccta ttatgtttat tagttcaaga240acagataata tggatcaaat tctggctatc caaatgggtg gcgatgattt tatcgagaaa300
ccatttaact tgtctttaac agtagctaaa attcaggcac tattgcgtcg cacttatgat360ttatcaatag ctcgtgatga aatagctgtt aaaggttgta aactaattgt tgatgaggcc420aagcttagca aggataatga acatgttcaa ctttccttaa cagagctaca gatattaaaa480ttgttatttc aaaatgaaaa taaatatgta agtcgtacag ctttaattga gaagtgttgg540gagtctgaga attttattga tgataatacg ctcgcagtaa atatgacacg attacgaaaa600aaattacttt ctatcggtgt cgatgattta attgagacaa agaaaaatgt aggatacagg660gtttga 66
權(quán)利要求
1.表皮葡萄球菌調(diào)節(jié)信號多肽，其特征在于包括組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白的16對雙組分調(diào)控信號蛋白，其中16個組氨酸蛋白激酶具有結(jié)合ATP的激酶活性功能域，分別具有SEQ ID No.1-16的序列結(jié)構(gòu)，其中16個反應(yīng)調(diào)節(jié)蛋白存在接受磷酸基團(tuán)的功能域，分別具有SEQ ID No.17-32的序列結(jié)構(gòu)。
2.根據(jù)權(quán)利要求1所述的表皮葡萄球菌調(diào)節(jié)信號多肽，其特征在于所述的組氨酸蛋白激酶結(jié)合ATP的激酶活性功能域是在AMP-PNP的結(jié)合部位形成袋狀結(jié)構(gòu)，其周圍有保守的基序圍繞，所述的反應(yīng)調(diào)節(jié)蛋白的接受磷酸基團(tuán)的功能域結(jié)構(gòu)由5個α螺旋和5個β折疊組成。
3.根據(jù)權(quán)利要求2所述的表皮葡萄球菌調(diào)節(jié)信號多肽，其特征在于所述的反應(yīng)調(diào)節(jié)蛋白的接受磷酸基團(tuán)的功能域結(jié)構(gòu)其中存在三個天冬氨酸殘基構(gòu)成接受磷酸基團(tuán)的活性位點(diǎn)，接受磷酸基團(tuán)的過程需要二價陽離子(Mg2+或Mn2+)的輔助，所述天冬氨酸殘基是D7、D8、D51。
4.根據(jù)權(quán)利要求1所述的表皮葡萄球菌調(diào)節(jié)信號多肽，其特征在于所述的組氨酸蛋白激酶的SEQ ID No.1-16的氨基酸序列結(jié)構(gòu)，由SEQ ID No.33-48的DNA核苷酸序列編碼；所述的反應(yīng)調(diào)節(jié)蛋白的SEQ ID No.17-32的氨基酸序列結(jié)構(gòu)，由SEQ ID No.49-64的DNA核苷酸序列編碼。
5.根據(jù)權(quán)利要求1所述的表皮葡萄球菌調(diào)節(jié)信號多肽，其特征在于還具有由SEQID No.1-32構(gòu)成的蛋白的氨基酸序列的同源序列及片斷序列，和SEQ ID No.33-64編碼相關(guān)同源序列和片斷有關(guān)多肽的DNA核苷酸序列。
6.根據(jù)權(quán)利要求5所述的表皮葡萄球菌調(diào)節(jié)信號多肽，其特征在于所述的同源DNA序列是編碼SEQ ID No.33-64所示核苷酸序列或片斷，或DNA片斷長度在60個連續(xù)核苷酸區(qū)域內(nèi)，至少70％同源或可與之選擇性雜交的片斷核苷酸序列；所述SEQ ID 1-32的多肽同源序列至少在30個連續(xù)氨基酸序列區(qū)域，至少60％以上的同源。
7.基于權(quán)利要求1所述的多肽，其多肽的磷酸化反應(yīng)產(chǎn)物。
8.權(quán)利要求1所述的多肽在制備抑制葡萄球菌生長制劑中的用途。
9.一種制備權(quán)利要求1的多肽的方法，包括基因克隆、基因表達(dá)、蛋白純化，其中基因克隆中將SEQ ID No.33-64 DNA序列與表達(dá)載體pet28a重組，制備相應(yīng)的重組表達(dá)質(zhì)粒。
全文摘要
本發(fā)明屬生物技術(shù)領(lǐng)域，涉及作為表皮葡萄球菌信號傳遞系統(tǒng)關(guān)鍵新蛋白組氨酸蛋白激酶和反應(yīng)調(diào)節(jié)蛋白；制備該類多肽的方法；編碼該類多肽的DNA；該類多肽的抗體；以及利用該類多肽結(jié)構(gòu)功能域篩選藥物化合物。本發(fā)明篩選的活性化合物，能阻斷組氨酸蛋白激酶或反應(yīng)調(diào)節(jié)蛋白質(zhì)間的磷酸基團(tuán)轉(zhuǎn)移和蛋白質(zhì)的磷酸化，抑制細(xì)菌細(xì)胞內(nèi)或細(xì)胞之間的信號傳遞，從而抑制葡萄球菌的生長。
文檔編號C12N9/48GK1528784SQ0315121
公開日2004年9月15日 申請日期2003年9月26日 優(yōu)先權(quán)日2003年9月26日
發(fā)明者瞿滌, 聞玉梅, 秦志強(qiáng), 陳潔敏, 瞿 滌 申請人:復(fù)旦大學(xué)