專(zhuān)利名稱(chēng)：一類(lèi)人類(lèi)肝臟相關(guān)基因的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物技術(shù)領(lǐng)域，具體地，涉及一類(lèi)基因，尤其涉及一類(lèi)人類(lèi)肝臟相關(guān)基因。
背景技術(shù)：
肝臟是人體內(nèi)最大的消化腺。也是體內(nèi)新陳代謝的中心站。據(jù)估計(jì)，在肝臟中發(fā)生的化學(xué)反應(yīng)有500種以上，實(shí)驗(yàn)證明，動(dòng)物在完全摘除肝臟后即使給予相應(yīng)的治療，最多也只能生存50多個(gè)小時(shí)。這說(shuō)明肝臟是維持生命活動(dòng)的一個(gè)必不可少的重要器官。肝臟的血流量極為豐富，約占心輸出量的1/4。每分鐘進(jìn)入肝臟的血流量為1000-1200ml。肝臟的主要功能是進(jìn)行糖的分解、貯存糖原；參與蛋白質(zhì)、脂肪、維生素、激素的代謝；解毒；分泌膽汁；吞噬、防御機(jī)能；制造凝血因子；調(diào)節(jié)血容量及水電解質(zhì)平衡；產(chǎn)生熱量等。在胚胎時(shí)期肝臟還有造血功能。
肝臟疫病分為肝炎、肝硬化、脂肪肝、肝癌等。現(xiàn)代醫(yī)學(xué)實(shí)驗(yàn)證明，肝病病毒侵入人體后，并不直接引起肝細(xì)胞的損害，只是在肝細(xì)胞內(nèi)吸收營(yíng)養(yǎng)賴(lài)以生存，并在肝細(xì)胞內(nèi)復(fù)制、繁殖。其復(fù)制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)釋放在肝細(xì)胞膜上，引起人體免疫系統(tǒng)對(duì)這些抗原物質(zhì)產(chǎn)生免疫反應(yīng)，這種反應(yīng)造成肝細(xì)胞的損傷、壞死。免疫反應(yīng)的強(qiáng)弱決定于肝臟受損程度及臨床癥狀輕重。這場(chǎng)由病毒引發(fā)的、免疫系統(tǒng)對(duì)肝細(xì)胞的戰(zhàn)爭(zhēng)，使大約25％的患者的肝臟成為戰(zhàn)火連綿的戰(zhàn)場(chǎng)，肝臟的損傷由此加重。肝病的危害絕不儀僅限于肝臟本身，它還可以引起其它多種疾病。常見(jiàn)的有(1)糖尿??；(2)胰腺炎；(3)膽道感染；(4)功能性腎衰竭；(5)膽汗性腎??；(6)腎小球腎炎；(7)腎小管酸中毒；(8)溶血性貧血；(9)再生障礙性貧血；(10)心肌炎和心包炎；(11)結(jié)節(jié)性動(dòng)脈炎；(12)消化性潰瘍；(13)自發(fā)性腹膜炎；(14)性激素代謝紊亂；(15)甲狀腺功能改變；(16)肝性骨病，等等。肝病不僅對(duì)患者的身體甚至生命造成危害，而且對(duì)患者心理上的打擊也是十分沉重的。無(wú)論是肝病患者還是病毒攜帶者，在生活、社交、求職、升學(xué)等方面都會(huì)受到嚴(yán)重影響。
生物基因組中可轉(zhuǎn)錄表達(dá)的序列(即基因)僅占總序列的3-5％，對(duì)這部分序列進(jìn)行測(cè)定，將直接導(dǎo)致新基因的發(fā)現(xiàn)，并獲取基因組中與產(chǎn)業(yè)化關(guān)系最為密切的信息。
人類(lèi)肝臟相關(guān)基因是指在人類(lèi)肝臟中表達(dá)的相關(guān)基因，其與一些肝臟疾病相關(guān)，因此，研究人類(lèi)肝臟相關(guān)基因?qū)μ剿鞲闻K疾病的發(fā)病機(jī)理及研制肝病的治療藥物具有重要意義。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問(wèn)題是提供一類(lèi)人類(lèi)肝臟相關(guān)基因。
本發(fā)明要解決的技術(shù)問(wèn)題通過(guò)如下技術(shù)方案實(shí)現(xiàn)本發(fā)明提供一類(lèi)人類(lèi)肝臟相關(guān)基因，其包括以下序列的基因(a)SEQ ID No.1～SEQ ID No.30所示的序列；(b)SEQ ID No.1～SEQ ID No.30所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.30所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.30所示的序列。
本發(fā)明還提供了一種探針?lè)肿?，所述的探針?lè)肿雍猩鲜鲂蛄兄屑s8-100個(gè)連續(xù)的核苷酸。
本發(fā)明的人類(lèi)肝臟相關(guān)基因，在研究肝臟疾病的致病機(jī)理以及開(kāi)發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
具體實(shí)施例方式
下面結(jié)合具體實(shí)施例，進(jìn)一步闡述本發(fā)明。應(yīng)理解，這些實(shí)施例僅用于說(shuō)明本發(fā)明而不是限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(cè)(New YorkCold Spring HarborLaboratory Press，1989)中所述的條件，或按照制造廠(chǎng)商所建議的條件。
實(shí)施例1人肝臟組織的mRNA的分離組織分離(Tissue isolation)肝臟來(lái)源于5個(gè)成年男性，在肝臟切除手術(shù)后，將肝臟組織立即置于液氮中冷凍保存。
mRNA的分離(mRNA isolation)取出肝臟組織，用研缽研碎，加入盛有裂解液的50ml管，充分振蕩后，再移入玻璃勻漿器內(nèi)，勻漿后移至50ml新管，抽提總RNA(TRIzol Reagents，Gibco，NY，USA)。用甲醛變性膠電泳鑒定總RNA質(zhì)量。用帶Oligod(T)的纖維素柱分離總RNA中的mRNA，定量。
實(shí)施例2cDNA文庫(kù)的構(gòu)建(Constuction of cDNA library)以mRNA為模板，合成雙鏈cDNA。補(bǔ)平末端后，加含EcoRI切點(diǎn)的接頭。磷酸化EcoRI末端后，用XhoI限制性?xún)?nèi)切酶消化1.5小時(shí)，再進(jìn)行片斷分離。過(guò)柱篩選長(zhǎng)度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，無(wú)菌水溶解，連接至Uni-ZAP XR載體(Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene，CA9203，USA)進(jìn)行包裝，宿主菌使用XL 1 Blue MRF’(Strategene，CA9203，USA)細(xì)菌。涂板并測(cè)定滴度。
實(shí)施例3測(cè)序及數(shù)據(jù)庫(kù)建立(Seqencing and Database Constructing)挑選文庫(kù)中有外源片段插入的克隆，擴(kuò)增后抽提質(zhì)粒(Qiagen Germany)，用T3和T7作為3’和5’端的通用引物，采用終止物熒光標(biāo)記(Big-Dye，Perkin-Elmer，USA)的方法，在A(yíng)BI 377測(cè)序儀(Perkin-Elmer，USA)上進(jìn)行EST大規(guī)模測(cè)序。測(cè)序結(jié)果用FACTURA軟件去除載體序列，傳輸?shù)絊UN Ultra 450Server上進(jìn)行下一步的處理。所有的序列信息再用GCG軟件包(Wisconsin group，USA)中的BLAST和FASTA軟件搜索已有的數(shù)據(jù)庫(kù)(Genebank+EMBL)，將無(wú)同源性或同源性低于95％的序列視為新基因建立數(shù)據(jù)庫(kù)。
實(shí)施例4基因的全長(zhǎng)克隆(Cloning of Full-length cDNA)在得到的新基因片段序列信息基礎(chǔ)上，進(jìn)行cDNA全長(zhǎng)克隆，分兩階段進(jìn)行(1)“電子克隆”(Electronic Cloning)以新基因片段序列作為探針?biāo)褜bEST數(shù)據(jù)庫(kù)，將重疊序列＞50bp，同源性在98％以上的表達(dá)序列標(biāo)簽(Expressed Sequence Tag，簡(jiǎn)稱(chēng)“EST”)序列認(rèn)為同一序列(Consensus Sequence)，取出并用AUTOASSEMBLER軟件進(jìn)行連接，部分EST可以延伸探針序列。再用STRIDER軟件分析被延伸的序列是否具有完整的開(kāi)放閱讀框架(OpenReading Frame，ORF)，用BLAST搜尋Genbank或SwissProt以確定該序列的核苷酸和氨基酸水平上是否與其他物種有同源性，以幫助判別所得到的基因全長(zhǎng)完整性如何。通過(guò)電子克隆的方法，通?？色@取人肝臟相關(guān)基因的全長(zhǎng)序列。
(2)cDNA末端快速擴(kuò)增(Rapid Amplification of cDNA Ends，RACE)如果通過(guò)“電子克隆”方法仍未得到完整的cDNA全長(zhǎng)，則在已有序列5’或3’端設(shè)計(jì)引物，在人類(lèi)肝臟Marathon-Ready cDNA文庫(kù)(Clontech Lab，Inc，USA)中進(jìn)行長(zhǎng)距離PCR反應(yīng)。然后對(duì)PCR產(chǎn)物克隆、測(cè)序。用AUTOASSEMBLER及STRIDER軟件分析被延長(zhǎng)的序列有無(wú)完整的ORF，如無(wú)，重復(fù)上述過(guò)程直至獲得全長(zhǎng)。
(3)RT-PCR對(duì)于5’和3’端的已知的序列，如果中間有一段間隙(gap)無(wú)法從已有的公共數(shù)據(jù)庫(kù)或自身數(shù)據(jù)庫(kù)獲得，可考慮采用RT-PCR的方法。在序列5’端設(shè)計(jì)引物，3’端引物采用Oligo-dT，在肝臟總RNA庫(kù)中進(jìn)行擴(kuò)增。然后對(duì)產(chǎn)物進(jìn)行克隆、測(cè)序。最后拼接便獲得全長(zhǎng)。
通過(guò)組合使用上述3種方法，可獲得人肝臟相關(guān)蛋白的全長(zhǎng)編碼序列。
序列表<110>上海人類(lèi)基因組研究中心<120>一類(lèi)人類(lèi)肝臟相關(guān)基因(6)<130>NP-10160<160>30<210>1<211>1747<212>DNA<213>Homo sapiens<400>11 tttttttttt tttgtgcatt aattatttta ttaatttctt ctttttacat tatgggaaac61 attcatctat ttacaatatt tttttgttca cacacaatct aggnaaataa gcctatgaaa121 ttccaattca taaagccata aaaatgttcc cacccctcca tctgaaggct ttcaaatgaa181 tctttttttt ccaaaccaaa ataattttta aaaattacat ttgggaattc agatatgcta241 gtgatttaca ttccagttat ttaaaatatg catctagaca tgtttaaaaa agaaatggta301 aattcacaag gataaggctt aaatgaaagt ggtaatgcac agtaaaacat taaggaaaca361 tctataaata acagaaatat attacaataa attagttcta tttaccattt caaatattaa421 aaatctttaa tccatttctt catctctctt tacaaaaagg ttatgtgaat tgtatggaaa481 catctgcaaa aaatataata aatacttaat ttctttgaac gttttttgnt gtgggagaca541 aacttctttt gtaatccccc cctccccaag aaaatgccca acacctttat accaaagtta601 aacaaaataa gtaatttttc agggtacata cattttccat tacgaactaa aaaacatttc661 tacaaattac atcacaaaga acaatgtaac anaggttatg ctcatgaatg tcagtttcag721 gggtcaagca gcattgtgtg gggtcatgtt ctaacaccaa taattagctt tttataaagt781 gtttgtncaa tttaaattgt attttcaagg aaggcaaaga tcatgatgcc ctgtaggcct841 tgagctaaaa gatttaaaat tcttaccaca aatagcttat gaatatacaa atgccgttca901 cttagacaag ataaataggc cttctcttgt ctctctttaa aatatcatgg gctaaattag961 tttccaaaga aaatattgta ggatgatctt gagtcgtatt agaaatctat ttcttaatcc1021 aaatctccaa ctctacctca gggcacggag ctgtaattgt gagcatgggt cttgggatga1081 acgctgcttc tttcatatta atctgtgagt tccaagtcat tctcagtatt gaatgtgacc1141 ataggctacc caagtggcag ttggccctac tcgatgcaag ctttgtgtga cttttaattg1201 ctgctacaag gtaaagaaaa ggtgtattct ttttaaaact agcaaatact tgaagctcaa1261 aatctctgta tgctccaatt tcacaaagca cttttgagtg ctttgttcac cctagttaaa1321 agttggttcg ttggtttcaa aataagtgag tagccctttt cttgtaaacc acagcagtaa1381 acatttgtgc cctgtttaat aaaggtagct caaagcatcg atggctctga gaggtagcga1441 attctctagt ggttttagaa tatgtccgtg aaaatatctg ttgctagaac ccatcacctc1501 tcttaatttc ataagtaaaa ttaaattagc acctggctat gagagtgtat tattttaaaa
1561 agcttctctg gtgcaagcat gtttgatgta tgtttttaaa agtctttcgt ttttaatttc1621 taatgcttta ggatgttagc tggacataaa atgccattga tacttaagat tgccacatcc1681 taagtgttta aactgtgacg agtaagggca tttaaagaca acttcagcta gacaccgatn1741 atactnc<210>2<211>1806<212>DNA<213>Homo sapiens<400>21 ccacgcgtcc ggtgggacag tgtctccagg tagtgaggcc caacctcttt aaaacaaaca61 aacaaacaaa caaaaaacca aaatgcaaac gtgagtagag aatgaaatac acagtttaaa121 tgccctcact atctttgggt tactaatata ctaagccttt ctggactaag taaaagatgc181 aggttcctgc ggctagggat agaagccaga ctggcgtctt cagaatctcc caccaaggct241 gtacctctgg aggagttcta ggtttggatt tggtcatctc aagaagagtc ttcagatgga301 ttttaaaatt gaacacactt gggatggttt tccagtgaag catgagcccg tatttatcag361 gctgaatcca ggtgacagag gagtgatgat ggacattagt gctccatttt tcagggatcc421 tccagcccca cttggagaac caggaaaacc tttcaatgaa ctgtgggatt atgaagttgt481 ggaagcattt ttcttgaatg atataactga gcaatattta gaagttgaac tttgtcccca541 cggacagcat ttagtgcttt tactttctgg aagaagaaat gtgtggaaac aagaacttcc601 tttatcgttc agagtgtcca gaggagagac aaaatgggaa ggcaaagctt atctcccttg661 gagttatttt ccaccaaatg tgacaaaatt caattcattt gcaattcatg gatcaaaaga721 taaacgaagt tatgaagctc tttaccctgt acctcagcat gaactgcagc aaggacaaaa781 acctgatttc cattgcctag aatacttcaa gtctttcaat tttaacacac tgcttggaga841 agagtggaaa caaccggaat cagacctgtg gctaatagag aaatgtgata tataggagta901 atagataacc ataccgatca ttttttcctc tatacctttt aagataaaca aaaaataaat961 atcaattttt taagatgtca tgcatacatt tcaacaacaa atatcttcat agaagtcact1021 gaaaatatag tatctgtggc aaattgtata tgattaacaa gaaaatatat gattctttgt1081 agatgatttc atctgtaaag tcacaatgtt tctcagagtc acttagtcca ggatgttgca1141 ttcccagcac acagtacagt tattcttgaa aactacaagt gaaaaagggg gaacaattga1201 ggttgtccaa ggccacctag aggactgtct tgcactacct tgtgattcaa ccatttcatt1261 cctagatata taccccagaa aaatgactgc tcatagcagc tttattcata atagcacaaa1321 attgtaaaca gtgaaatttc catccatagg aaaatggata gacaaactac aatatagtca1381 gtggaatact gttcatttaa aaaaggactg ctgatacatg caacaacctg tatgaatctc1441 aaaaacatta agttgagcaa aacaaaccaa acataacata ctatctaatt acttttatat1501 taaagccact tccaggcaaa actaatattt gatgatagat accagaacag tggctgctta1561 gagtgagggc agggaattca aattgaatgg aaaaggggcc tgaggaaatt ttctggggtg1621 attgaaatgt tttgtatttt gatctggatg atggttatgt agctgcatat atttgtcaac1681 attcctcatg ctgtatgttt agatttgtga ttttactata tgtaaattaa ttccttaata1741 aaatactatt ataaaataag ctgtaaatta aatggaatta acttcaagtt taaaaaaaaa1801 aaaaaa
<210>3<211>2398<212>DNA<213>Homo sapiens<400>31 ccacgcgtcc ggcagcggcc tcgggggttg gggggctgtg tgagtctcgc agtggggctg61 aggcaggcag ccgggagagc catggcgggg gccgcagcgg gcggcagagg cggaggtgcc121 tgggggccgg ggcgcggagg ggccgggggg ctccggcggg gctgctctcc cccagccccc181 gccggctccc cccgggctgg gctgcagccg ctcagggcca cgatcccctt ccagctgcag241 cagccgcacc agcgccggga cgggggtggc cgtgcagcca gcgtcccatg ctcggtggcc301 ccagaaaagt cagtgtgtag gcctcagcca cttcaggtcc ggcgtacatt ctccctggac361 accatcctca gctcctacct tctgggccag tggccacgag atgctgatgg ggccttcacc421 tgctgcacca atgacaaggc cacccagacg cccctgtcct ggcaagagct agaaggtgag481 cgtgccagtt cctgtgcaca caagcgctca gcatcctggg gcagcacaga ccaccgaaaa541 gagatttcca agttgaagca acaactgcag aggacgaagc tgagccgcag tgggaaagag601 aaggagcgag gttcaccact cctaggggac cacgcagtgc ggggagcact gagggcgtcc661 cctcccagct tcccctcagg gtcccctgtc ttgcgactca gcccctgcct gcacaggagc721 ctggaagggc tcaaccaaga gctggaggag gtatttgtga aggagcaggg agaagaggag781 ctgctgagga tccttgatat ccctgatggg caccgggccc cagctcctcc ccagagtggc841 agctgtgatc atcccctcct cctcctggag cctggcaacc ttgccagctc tccttccatg901 tccttggcat ctccccagcc ttgtggcctg gccagtcatg aggaacatcg gggtgccgcc961 gaggagctgg catccacccc caacgacaaa gcctcctctc caggacaccc agcctttctt1021 gaagatggca gcccatctcc agtccttgcc tttgctgcct cccctcgacc taatcatagc1081 tacatcttca aacgggagcc cccagaaggc tgtgagaaag tgcgtgtgtt tgaagaagcc1141 acgtctccag gtcctgacct ggccttcctg acttcctgtc ctgacaagaa caaagtccat1201 ttcaacccga ctggctcagc cttctgcccc gtcaacctga tgaagcccct cttccccggc1261 atgggcttca tcttccgtaa ctgcccctca aacccgggat ctccccttcc cccggccagc1321 cccaggccac cacctcggaa ggatccggaa gcctccaagg cctccccact gccattcgag1381 ccatggcagc gcaccccacc atcagaagag cctgtgcttt tccagagctc cctgatggtc1441 tgagggtccc acccctgccc cactttacca tagagaccag tgccttggtg gcaggtccct1501 ccccaggtcc cctgagatgg ggtatggagg ggcccttccc tctcggcctt cgagcacttt1561 ctttcactta ctgtgtcaaa gccctgggtc ctctttttga tgggcaccgg cccctctgaa1621 cgtgatggga cctgccttct ccactagtag ctgggcagct cacaattcac acctgtgtac1681 ctgccacatc cctcacttgg tggaaaacac ccagaaggtc ttgagtcccc cacccctggg1741 tgtcagtcca aatgactgta taggaggccc ttatttttgt cacagagcaa gctggccatg1801 aacgaaggag agaagacgcc acagatttcc ttccctctcc tccaggagac cataagatag1861 atcccccatc ctctcagccc tattcccatg cctccctctc attggaggag ctgaccaaag1921 cagccctaac gggccataac acttgaccaa ttcagctgct ggcagaggga ggaaacaagt1981 gttttcccaa gtggcatttt catctcgctt tcaccctgac taaagattgt cttaagtagc2041 agcccagccc gcccagcccc aggtgggtag tggggaggag agctggcatt cctccaggtg2101 gcaaatggcg actctatact ctccgcccgc cccagggctg gatggattag aaaaatgcct2161 atttttcttg tatcgatgta gagactctat tttctcccaa agacactatt tttgcagctg
2221 tttgaagttt gtatattttc cgtactgcag agcttacaca aaattgaaga atgttaatgt2281 tcgagttttc ttatcttgtg tttagaggtt gttttttgca gatcttggtg ttaatagacc2341 aaataaataa ataaatattc ccagcagctt gaaaaaaaaa aaaaaaaaaa aaaaaaaa<210>4<211>2652<212>DNA<213>Homo sapiens<400>41 ctaaatttat cttcagaccg gcgaccgcgc cagccgcgaa tcggatctct atggagatgg61 cctaggcgat atccctgagc tgagagcatc accctgtccc cgaatccttc tttcctctct121 gttttgtttt tcattcccct ccctctctcc cctccccctc cagtccacga cgactggctg181 ttgaccctgt tcaggcctcg gtgaaggctt ttggttactc cccttcccac cccatccctt241 aattttattc ttttgaagag tgcatttcaa gctgccaagg tggagagagg gattacagaa301 aggagaacac cttatttcag cttttatttt tatgtgataa tctaccttta agaaaaggtg361 taccatacct gagagcacca ggaagtcgca tgagagatca cctgatacat gaacgtatga421 tgttccatct gcgcattgat gaataggcag catttacaaa ttaactgatg tgttgctgta481 tatcatctct ttgatgattg ctcctcttct ttgtatcctg tcttataatt tcaacacatt541 tgcgatactc aatgtctatt ctaaattaac catgttttgt accacaaact cattgcccat601 ggatctgttg ctgaaacaag gaagtcttaa acaagaagtg gaatctttct gttatcagat661 tgtgtctgaa tcaaatgatc agaaggttgg aatattacaa agtgaagata agcagttgca721 accttcagtt tctaaaaaat cagaaggtga gctttccagg gtcaaattta tatccaattc781 caacaaaata acatttagta aaaaaccaaa aagaagaaaa tatgatgaaa gttatttgtc841 ttttggattt acttacttcg gaaatagaga tgcacctcat gctcagtgtg tattatgtaa901 gaaaatttta tcaaatagct ctttagcccc tagtaagctt cgaagacatt tggaaactaa961 acatgctgca tataaagaca aagacataag ctttttcaag caacatctcg attcacctga1021 aaataataaa cccccaacac ctaaaattgt gaatacagat aatgaaagtg ctacagaagc1081 atcatacaat gtaagttacc atatagcgct gagtggagag gctcatacta ttggagaatt1141 gcttatcaaa ccttgtgcaa aagatgtagt gatgcggatg tttgatgaac aatatagtaa1201 aaaaatagat gcagtacagc tatcaaacag tactgttgca cgtcgaatta aggatctagc1261 tgctgacatt gaagaagagc ttgtttgtag actgaaaatt tgcgatgggt tttcactgca1321 actagatgaa tcagctgatg tttcaggact tgctgtgctg cttgtgtttg ttcgttatag1381 gtttaataag tctattgagg aagacctact cctgtgtgaa tctttgcaaa gtaatgctac1441 cggtgaagaa atattcaact gtatcaacag ttttatgcag aaacatgaaa ttgaatggga1501 aaaatgtgtt gatgtttgta gtgatgcttc tagggcagtg gatgggaaaa ttgccgaagc1561 tgtcacctta ataaaatatg tggctcccga aagcaccagt agtcactgcc tattatacag1621 acatgcactg gcagttaaaa taatgcctac atctctaaaa aatgtgctag accaggcagt1681 acaaatcatc aattatatta aagctcgacc acatcaatcc agactattaa aaattttatg1741 tgaggaaatg ggtgctcagc acacagcact tcttctaaat acagaggtga ggtggctttc1801 tcgaggtaaa gttcttgtaa gactttttga acttcgtcgt gaacttttgg ttttcatgga1861 ttctgctttt cgactatctg attgtttaac aaattcatct tggctgctaa gacttgcata1921 tcttgcagat atttttacta aattaaatga agttaatttg tcaatgcaag gaaaaaatgt
1981 gaccgttttt acagtatttg ataaaatgtc gtcattgtta agaaaattgg aattttgggc2041 ctcatctgta gaagaagaaa actttgattg ttttcctaca ctcagtgatt ttttgactga2101 aattaattct acagttgata aagatatttg cagtgccatt gtgcagcacc taaggggttt2161 gcgcgctact ctgttaaaat actttcctgt aacaaatgac aataatgctt gggttagaaa2221 tccatttaca gttactgtta aaccagcttc attagtagca cgggactatg agagcctgat2281 tgatttaaca tctgattctc aagtgaagca aaattttagt gaactttcac taaatgattt2341 ttggagtagc ctaattcagg aatacccaag cattgcaagg cgtgcagtgc gtgtacttct2401 tccttttgct acaatgcacc tgtgtgaaac ggggttttca tattacgctg caacaaaaac2461 aaaatatagg aaaagacttg atgctgcacc tcatatgcga atccgactta gcaatattac2521 acctaatatt aagcggatat gtgataaaaa gacacaaaaa cactgttctc attaaaattg2581 gaggagtttg catgtctcat gataaccaaa tgtaagatga aaataaaaga tgatttactt2641 caaaaaaaaa aa<210>5<211>1994<212>DNA<213>Homo sapiens<400>51 tggcttctgc gtggtgcagc tgcgcacgtg tttcagccgg cagcgcttta agatttccgg61 ggatggaatc cgaaatggaa acgcagagcg ccagggcaga ggagggcttt acccaggtca121 cccgcaaggg tggccgacgg gcgaagaaac gacaggctga acagctgtcc gcagcaggag181 agggcgggga tgcgggccgc atggacacag aggaggccag gccggcgaag aggcccgtct241 tcccacccct ctgtggggac gggctcctga gtgggaaaga agaaacaagg aaaattccag301 tcccagctaa cagatacaca ccattgaaag aaaactggat gaagatattt actcctattg361 tggaacattt gggacttcag atacgcttta acttgaaatc aaggaatgta gaaatcagga421 cttgtaaaga aaccaaggat gttagtgctc tgacaaaagc agctgatttt gtgaaagctt481 ttattctcgg ctttcaggtg gaggatgcac ttgccctcat caggttggat gacctcttcc541 tagagtcttt tgaaattaca gatgttaaac ccctaaaggg agaccatcta tccagggcaa601 taggaagaat cgctggcaaa ggaggaaaaa ccaaattcac catagagaat gtgacacgga661 caaggatagt tttggctgat gtgaaagttc acatccttgg ctccttccaa aatatcaaga721 tggcaagaac tgccatttgc aacctaatct tgggaaatcc tccttccaag gtttatggca781 atattcgagc tgtggctagc agatcagcag atcgattctg atttcaagtc agagactttt841 tatcttgcct ttggactctg gtgaaaaata ctttacagtg gtcggtcaca agaaaccagc901 tgaacaattt cagtcatttg aagcctccgt cccttcttcc attctcagcc agaagcataa961 acagaaaaga aagatttaag aggattcaca ctcaacaggt tttaggataa tttaaatatc1021 aaaaattgat tgttatactt aacacattag gtataattta tcatttatct gaaatcacat1081 gtagcagatt gcatagtctg taatcctctc agagggaaac ttcttgttta aacagctcta1141 tatggattta tacttttata tttataaatt tataacttca tacaaattta taaacatttc1201 tttataaatt gtaatttaat agattatctc agaaaaacct ctctgaatga tgacccttcc1261 ttaatactgg gtgatgtgtg aatatttgtt tgttggcaga cagggtctca ctttgtcacc1321 caggctagag tgcagtggtg caatctcagc tcactgcagc ctctgcctcc tgagttcaag1381 tgatcctcct gcttcagcct ccctagtagc tgggactaca ggtatgcacc accatgcctg
1441 gctaattttt tgtatttttt gtagagatgg ggtttcgcca tgttgaccag gctggtcttg1501 aactcctggc ctcaagggat ccgtccgcct cagcctccca aagtgctggg attacaggca1561 taagccactg cgcctggcct tgatgtgtga atatttgaga ggtcataagc agtggttttg1621 gccataccgt attataccat atacatcagt aagagctcat cttggaacct gttttgcagt1681 ttcttgcagt ttcaaaaatt aaagacctac atcacagggt taatgtgaag aaagctttat1741 ttttcaaatg agtatttaat gaaagtatac ataaccaatg ttgggtatac agatgcttct1801 caacttatga tgggtttagg tccagataag cccactgtga gttgaaaata ccaaaagtca1861 aacatcatag cttagcctac cttaaaaggg ctccgaacac tacattagcc tacagttggg1921 caaaatcgtc taatacaaag cctattttat aataaagtat gttgaatatc tcatgtaaaa1981 aaaaaaaaaa aaaa<210>6<211>1316<212>DNA<213>Homo sapiens<400>61 ggtaggtccc ggcaaccgca gctcgcggcg ggcgctgggc gggatccgac tctagtcgta61 atggaggcgg gcggctttct ggactcgctc atttacggag catgcgtggt cttcaccctt121 ggcatgttct ccgccggcct ctcggacctc aggcacatgc gaatgacccg gagtgtggac181 aacgtcagat tcctgccctt tctcaccacg gaagtcaaca acctgggctg gctgagttat241 ggggctttga agggagacgg gatcctcatc gtcgtcaaca cagtgggtgc tgcgcttcag301 accctgtata tcttggcata tctgcattac tgccctcgga agcgtgttgt gctcctacag361 actgcaaccc tgctaggggt ccttctcctg ggttatggct acttttggct cctggtaccc421 aaccctgagg cccggcttca gcagttgggc ctcttctgca gtgtcttcac catcagcatg481 tacctctcac cactggctga cttggctaag gtgattcaaa ctaaatcaac ccaatgtctc541 tcctacccac tcaccattgc tacccttctc acctctgcct cctggtgcct ctatgggttt601 cgactcagag atccctatat catggtgtcc aactttccag gaatcgtcac cagctttatc661 cgcttctggc ttttctggaa gtacccccag gagcaagaca ggaactactg gctcctgcaa721 acctgaggct gctcatctga ccactgggca ccttagtgcc aacctgaacc aaagagacct781 ccttgtttca gctgggcctg ctgtccagct tcccaggtgc agtgggttgt gggaacaaga841 gatgactttg aggataaaag gaccaaagaa aaagctttac ttagatgatt gattggggcc901 taggagatga aatcactttt tattttttag agattttttt ttttaatttt ggaggttggg961 gtgcaatctt tagaatatgc cttaaaaggc cgggcgcggt ggctcacgcc tgtaatccca1021 gcactttggg aggccaaggt gggcggatcg cctgaggtca ggagttcaag accaacctga1081 ctaacatggt gaaaccccat ctctactaaa aatacaaaat tagccaggca tgatggcaca1141 tgcctgtaat cccagatact tgggaggctg aggcaggaga attgcttgaa cccaggaggt1201 ggaggttgca gtgagctgag atcgtgccat tgtgatatga atatgcctta tatgctgata1261 tgaatatgcc ttaaaataaa gtgttcccca cccctgaaaa aaaaaaaaaa aaaaaa<210>7<211>1980
<212>DNA<213>Homo sapiens<400>71 ggcgtaaggg gcgtggcgcc agtgggcgtg gcgtggcgca gtgcgaaggg acgcggtgcg61 catgcgcgtg agggctgccg cgggtgggtg gtatcgaggc ctgtcgggtc agggcggttc121 gcgggtgctg tcagagctgg gccggggccc ctaggcaggg tagccgggtc gtagaggcgg181 gggccggtcg cggtcggtgg agcgggatga ggatgtagga ggggcggacg tggcggaagc241 cgcggggtcc gcggggtcgg tgcctctagg gagccaggga ggcctttccc gaggctcctg301 gggaagaaga ggcgaagcga gagtccctgg ggaaccccca ctccactccc agctggagac361 tgggttgtgt ctgcatggac cagagcccac agtgcgagtt gctataggca accagccagg421 gtggccagct ccttcccgtt tgcccgtgat gttctggttt tgggaccaaa gcatcctagg481 cctccagccc actgcagtga ccgaattctg cgccccctgc ccatcttctc ccgcagcttc541 cctagattag gcttgggagg caagaggagg cctcctgacc tttcacactg cctttttaat601 attaagatga agtcacactc cacaactttc ttccagccag gcccagacat gtccgtcctt661 gtaagttaaa agcttccatg ggagccttcc ttcctaatca agatgcaaat aatacggcac721 tccgaacaga cactaaaaac agctctcatc tcaaagaacc cagtgcttgt atcacagtat781 gagaaattaa atgctgggga acaacgttta atgaatgaag ccttccagcc agccagtgat841 ctctttggac ccattacctt gcattctcca tcagattgga tcacctccca ccctgaggct901 ccccaagact ttgaacagtt cttcagtgat ccttacagaa agacaccctc tccaaacaaa961 cgcagcattt atatacagtc cattggctct ctaggaaaca ccagaattat cagtgaagaa1021 tatattaaat ggctcacggg ctactgtaaa gcatatttct atggcttgag agtaaaactc1081 ctagaaccag ttcctgtttc tgtaacaaga tgttccttta gagtcaatga gaacacacac1141 aacctacaaa ttcatgcagg ggacatcctg aagttcttga aaaagaagaa acctgaagat1201 gccttctgtg ttgtgggaat aacaatgatt gatctttacc caagagactc gtggaatttt1261 gtctttggac aggcctcttt gacagatggt gtggggatat tcagctttgc caggtatggc1321 agtgattttt atagcatgca ctataaaggc aaagtgaaga agctcaagaa aacatcttca1381 agtgactatt caattttcga caactattat attccagaaa taactagtgt tttactactt1441 cgatcctgta agactttaac ccatgagatc ggacacatat ttggactgcg acactgccag1501 tggcttgcat gcctcatgca aggctccaac cacttggaag aagctgaccg gcgccctcta1561 aacctttgcc ctatctgttt gcacaagttg cagtgtgctg ttggcttcag cattgtagaa1621 agatacaaag cactggtgag gtggattgat gatgaatctt ctgacacacc tggagcaact1681 ccagaacaca gtcacgagga taatgggaat ttaccgaaac ccgtggaagc ctttaaggaa1741 tggaaagagt ggataataaa atgcctggct gttctccaaa aatgaggacc ttcaaatagg1801 agtgattgaa ataaataact acttgcatgt tatgctttca tttgggtgga atacttcatt1861 ggaataaact actgatcttg tgctgtgtca aagtaacaga ctagaacctt ctttcaagta1921 cctgaattga aatgaaactc attttgaata ataaaaactc tagaaactct ttatcttctc<210>8<211>1774<212>DNA<213>Homo sapiens
<400>81 tgttcctgcc tctccgccac ctccaccgcg gcttatcccc tggctggcgg cgttggcggg61 gcgggggaca gtagttgtag acgcccgccc ctgcctcaga gaagatacca cattgaatga121 acaaagactg aactttgttt acagatacct tttataaaag atgtctgtgg agtagtgtta181 cagttcaaat aacaaaggat tttgcattat gagatgagtg agagtggaga attggacaaa241 aattgatttt tgatctctga attgtggttt ttagctcacc agttattgtg tagtctcact301 atttgaaaat tcatgctgaa aaactgagtg aatatgatca gatttctcca ccaaattctc361 agcctgataa ggaaaatccc gttttgtcaa cctaatgaag cattcaaaga agacatatga421 ctcttttcaa gatgaacttg aagattatat taaagtacag aaagccagag gcttagagcc481 aaagacttgt ttcagaaaga tgaaagggga ctatttggaa acctgtgggt acaaaggaga541 ggttaattcc agacccacgt atagaatgtt tgaccagaga ctcccatctg aaaccatcca601 gacctaccca agatcatgca atattccaca aacagtggaa aatcggttgc ctcagtggtt661 accagcccat gacagcagat tgagactaga ctctctgagc tactgtcagt tcacgaggga721 ctgtttctca gaaaaaccag tacccctgaa ctttaatcaa caagaatata tttgtggctc781 acatggtgta gaacatagag tttacaagca cttctcctca gataacagta ccagtactca841 tcaagccagt cacaaacaga tacatcagaa gaggaaaagg cacccagagg aaggcagaga901 aaaatcagag gaggagcggt ctaagcataa gagaaaaaaa agctgcgagg aaattgactt961 agacaaacac aagagcatcc aaagaaagaa aacagaggtg gaaatagaaa ccgtacatgt1021 cagtacagaa aagcttaaga atcgaaagga gaaaaaaagc cgagatgtag tctctaagaa1081 agaggaacgt aagcgtacaa aaaagaaaaa ggaacaaggc caagaaagga cagaggagga1141 aatgctttgg gaccagtcta ttcttggatt ttgaagcttt caaagttggt tctcccaaag1201 ttaaattgaa aaaataggtg agagcttggt tttatgatat ccgtgttcat accacttttc1261 ttatgtgaat aggttcttta acttctaaca aaggcctagt aaacaaagtg tttagcatgc1321 ttgctctcca acacagaaat tgcttttcct cattttctaa aagcattatt acattttttg1381 aacatatagt gtaatttcct ttaatgaaag tgactctgct tttattcatc aaattgcttt1441 gatggtggaa atattttctg ttgggaggtt atttatttta aattggagga ttaatgacct1501 ttgcacaatc tgtttcttga ttgggtttgt tatagttttg agttgggtat tttatgttca1561 ttggtttttc tctgtgaagc aatttttttc tcctttatta gatctaactt gcagtgtatt1621 ttctaggctg gaaagtggaa aatgaaatat attatgatct taggttacat aaagtttcta1681 aagtttcaaa gagtcttgat acaaaatcag tttatattct gaaaatattt ataataaagt1741 attctaattt ctaaaaaaaa aaaaaaaaaa aaaa<210>9<211>2441<212>DNA<213>Homo sapiens<400>91 gggccacaca gcggacccct aagcggaccg ctggcaccgg tcgggtggca gcagagtgtc61 gctcgacatg ggggaggccg gggctggcgc tggcgcctcg ggagggccgg aggcaagccc121 ggaggcagag gtggtgaagc tgctgccctt cctggcgccg ggcgcgcggg cggacctgca181 ggcggcggcg gtgcggcacg tgctggcgct gactggctgc ggacccggcc gcgcgctgtt241 ggcggggcag gcggcgctgc tgcaggcgct gatggagctg gcgccggcct ctgccccggc
301 ccgggacgcc gcccgcgcgc tcgtgaactt ggccgccgac cccggcctgc acgagacatt361 gctggcggcc gaccccgggc tgccagcgcg cctgatgggc cgcgcgttgg acccgcagtg421 gccctgggcc gaggaggcgg ccgccgcgct agccaacctc agtcgcgagc cggcgccgtg481 tgcagcgctc atggcggcgc tggcggccgc ggagccggca gactcgggcc tggagcggct541 ggtgcgcgcg ctgtgcacgc ccggctacaa cgcccgcgcg cccctgcact acctagcgcc601 gctgctctcc aacctcagcc aacgccctgc ggcgcgggcc ttcctactgg accccgacag661 gtgcgtggtc cagcggctgc tgccccttac ccagtacccc gactcctctg tacgcagggg721 cggggtggtg gggacgctgc ggaattgctg cttcgagcac cgacatcacg agtggttgct781 tggacctgag gtggacattc tccccttttt gctcctgccc ttggctgggc ctgaggattt841 ctccgaggaa gagatggaac ggctgcctgt cgacttgcag tacctgccac cagacaagca901 gcgagaacct gatgcagaca tccgcaagat gcttgttgaa gccatcatgc tgctgacagc961 cacagcacca ggtcggcagc aggtgcggga ccagggagcc tacctgatcc ttcgagagct1021 gcacagctgg gagccggagc ccgacgtgcg gacggcttgt gagaagctca tccaggtgct1081 tattggggac gagcctgaac gtggcatgga aaacctgctg gaggtgcagg tgcctgagga1141 tgtggagcag cagctgcagc agctggattg cagggagcag gagcagttgg agcgggagct1201 ggccccagag ccatgggtgg agagggccac acccacctga ggcccctgca gccggacacc1261 agctccaggg tcccctttgc caggctttct cagaccaggc cttcctgcag ctgtctgcag1321 gccccctggg tccccttgtt cagagactgt gctcttctga gaagcagagc tacgcttagg1381 ctccagtaag gattggaggc actttccagc cctgtgcagt gttgctacca gcaagaatga1441 aggttgtgca gagcagtact gtgaggtagg caccgtgact cggctgcctg tgcagaaggt1501 ggaggtggca ggcggatggc cagggagcca tgagaagact cttcctgggc acggcgtggg1561 gactggacag aaaaccactg caagccccaa ggagccagct gaagcctccc aatccccagg1621 gcactgcaga gtctgggcag aggtgggcgc ataggtacaa gtgctgcttt gtcagcctca1681 ctccctgcag ctctcagctc cctgttccct gagacctggc ctcaaagggc tgacccagcc1741 atacgtagat cctggggtct ttctaggact gtgcctccct gcttgaccta tgctccagga1801 ccctgccctc aacccttgtc agctccctaa gtcttcctgt ttcccagtct cagattgagt1861 gggtgctctc attggcctct ctagctaggc cgtctccctg gtcagcttcc ttgagcctgg1921 gccactcctc cctggccttc ttacctctgc caagagcctc cagatgtgcc tgcagcctag1981 tccctgccca agcttctccc ccagccaccc tctgcccctg tggtagagcc cactataggc2041 ccaggcgcct tacgtgcttc tcccctggac ctatgtgcat ccctcacctg ccagttatgt2101 ggctgccaag ccccacaggc tgtcatggtg gctcttgcca cacttccctt cctgagctga2161 cctgacctca ctgccacctc ttgcattggg atcctgaggg ctggcccctg ctcactgatg2221 ggccctccca ggcccgtcaa gccctggtga aggagcttag cttgtcttct tggcaattca2281 caatgcctca ctccacaccc taaaactgga gcgtccccta tggtctcctg ctgtggcttg2341 ggtgccgggc gtctcccgcc tccaggatct cctctcacct gcccccatta aagctctgcc2401 actcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa c<210>10<211>1491<212>DNA<213>Homo sapiens<400>10
1 caagggcccc cagtcccggg tgccgcgggg acagtgtagg gtcattagct gttgagccgc61 cccgggcggg cccaagcctt tggatctcag gtgaccggca caggcggccg cggggtccgg121 aactgcttgt tccggcagtg gaagagacgc gccggcgttg gccgctgctg ctagcagctt181 gaaccccagg gtcgggaccg atgtcggctt gggctgctgc cagcctaagc agggccgctg241 cccgatgctt gctggcacga ggccccgggg tcagggcggc tcctccgcgc gacccccggc301 cctcccaccc cgagccccgg ggctgcggtg ccgctccggg caggacgctg cactttaccg361 cggctgtccc cgccgggcac aacaagtggt ccaaagtcag gcacatcaag ggtccgaagg421 acgtcgaaag gagtcgcatc ttctccaaac tctgtttgaa catccgcctg gcagtgaaag481 aaggaggccc caaccctgag cacaacagca acctggccaa tatcttagag gtgtgtcgca541 gcaaacatat gcccaagtca acgattgaga cagcactgaa aatggagaaa tccaaggaca601 cttatttgct gtatgagggt cgaggccctg gtggctcttc tctgctcatc gaggcattat661 ctaacagtag ccacaagtgc caagcagaca ttagacatat cctgaataag aatggaggag721 tgatggctgt aggagctcgt cactcttttg acaaaaaggg ggtgattgtg gttgaagtgg781 aggacagaga gaagaaggct gtgaacctag agcgtgccct ggagatggca atcgaagcag841 gagctgagga tgtcaaggaa actgaagatg aagaagaaag gaacgttttt aaatttattt901 gtgatgcctc ttcactgcac caagtgagga agaagctgga ctccctgggc ctgtgttctg961 tgtcctgtgc actagagttc atccccaact caaaggtgca gctggctgag cccgacctgg1021 aacaggccgc acatctcatt caggctctca gcaaccacga ggatgtgatt cacgtctatg1081 ataacattga ataaccaggc tacatgtgcc cccgggttcc ttcctagaaa tgtggcagcc1141 cattccagca cacaggcttc tgcagcaatc tctgagggta aagccggtgg gaggctcagc1201 aggccaggag gcccaaggac aggacttgcg accttgaagc caaaggaatc tcacttgtgg1261 ggcctccttg tcagctctgc tgctgtctca gagccatctg gatgagtgtc ccgacaccct1321 ctcggatgca gggcaggacc acccagctgg tcagactctg atgttgggta gctggcctct1381 gtggggattg taagtgccct gaggcgctct gtactagaaa ctgctcttaa taataacggt1441 gattattggt tgctgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a<210>11<211>2625<212>DNA<213>Homo sapiens<400>111 gggggcgcgg cggcggcggg cgtgcgaggc tgttgtgctc ccggctctcg tgtttcccct61 cctgagcggg tggaggaggc ccaagcggtg ctgggcgcgc tcccccttcc tttccctccg121 gcgtcctctc ccggccctct cgcgctgcac tgtctctccg acgcaagact gtcccggccc181 ggatatggct cgtggacagc agaaaattca gtctcagcag aaaaatgcca aaaagcaagc241 tggacaaaag aagaaacaag gacatgacca aaaggctgct gccaaagctg ccttaatata301 tacctgcact gtctgtagga cacaaatgcc agaccctaag accttcaagc agcactttga361 gagcaagcat cctaagactc cacttcctcc agaattagct gatgttcagg cataaggttg421 tttacaggtg aattcatgac acctttgact cttctactgt ctcagacctt aggtaacata481 cctgcagctg cttttctaac aaactgttga tcagcaaaaa taaaggggct acagaaacac541 tcatttttat gctgttccct cttgggcttc atgcaaagac aattctgtgt aaatgtacag601 ttgactctga tttggaaata tgaaaatcag tccatccttg ttatgaaaat ttttttacaa
661 ttgtaattat attgatgttc atattgtgta aaataactca tttaataaaa tagtactttg721 atttacgaca tcacaggata aatggtttta gaaattctgt tctaactttc cacattattt781 gccttataaa aatctaatga attcatcagc tagaattgca agtgcaattc ttatatccct841 ttctctgctc agtggcaggt tcctcagtta aactagagca gactgattca ttaaaattgt901 gcatacgatt ttatgggcag ctgatgatct aggtgaaaaa tgacttatct gctgccttag961 tatattgcgg ttatgtgtca ttgtacccct ctgatcattt cctgtgtttg agttggaata1021 tttaaaattg cagtatgacc tggctctaac tgccagtcag gtgtaccctg tagataactg1081 atagcttcct aaaagcggtt ggattgtcag tgagcccttg tgaaaggtta ggttctaatg1141 tatatgccgt aatgaaataa tcattaagcc tattgtttaa tgcaaaatat gggacaaatg1201 tgaactggta aaggtcgatc ttgatactat attctttgaa aattcttgaa gttctttaat1261 ttgaaattga aacattaatt tttgaggttt ttggaagtta ctatttggcc atttttacaa1321 atggattttg cattaacagg aagattggaa tgacttgtgg ctggattaat tcccagaacc1381 ctctccgtcc tttcttttct aagtgagttg ttgattttag gagtttgctt tgtgctgtta1441 cctaaaatca gagactggtg tacagtatga cttccatctg aacttgaaaa gtattaaaat1501 gggtcaatat tcacattctt ttagatctca ggatgggaaa atgggtttgt tcagatggat1561 atggtaaatc ttacccaatt aaggaaggct aattttagag ttcttctaat ttgcttgtgt1621 caactcttaa cttgtaaaac tgctgttaac tcggtttaaa tttttaaatt aatataatag1681 aaagaacact aaaatactta ctggtaactg cttagagcca tttatctgat cctgtggatt1741 acagaatgca ttccctcttc tgctgtagct tatcattgct ttgttttgta gctctgcata1801 ttttgcccac cttgtcttcc ctgtaagttt aagacgtttc caaggaaaag cttcagggct1861 gatagtttct tagcaattcc tattcttatg cccttacttt aaaatagtcc ttttattttg1921 tgtattttat ttcctaagtt tcagatgtaa tatctgttgc ttcctaacat gtcgattacc1981 agaacgttag aattttacct aatttcttgt ggatattgca gaagttctgg ttaaatcaca2041 acttaaaagt ttttaaaagt gctttgagca tatgtatatg tttagtgaca aatcatataa2101 aaccattcac aagttttggt tttttttttt cgtttgtttt agtgactaat tcaggatgaa2161 agtttttttg ttcttgtatt taaccctttt ttatgagcag ctgaagacac catatttaac2221 actatatctc agtgataggg aaatagctgc attgatctta catgagcata atcatcctta2281 tacttcatga ggggattatt agtacaatcc ccattttact gtgtttgagt taaaaaccaa2341 acatccctgt aatttaattt gaagattctt taacagattg cagcaaagtt cattataaaa2401 ctgttatggt gtcttcaaag acttgataaa ataacactga gagagaattg gtccatttgt2461 atgctgtatt tctattactt gccaaaagga atggggttaa gattaaactt gtttccattc2521 tcttcacatg gatatacatc cccatgttta actgacacac tgggggctca gttgtgtgct2581 gtaatgtctt attaaagaag atattaaaga aaaaaaaaaa aaaaa<210>12<211>1307<212>DNA<213>Homo sapiens<400>121 ggctgctata gagccgggtg agagagcgag cgcccgtcgg cgggtgtcga gggcgggttg61 cctcgcgctg acccttcccg ccctccttct cgtcacacac caggtccccg cggaagccgc121 ggtgtcggcg ccatggcgga gctgacggct cttgagagtc tcatcgagat gggcttcccc
181 aggggacgcg cggagaaggc tctggccctc acagggaacc agggcatcga ggctgcgatg241 gactggctga tggagcacga agacgacccc gatgtggacg agcctttaga gactcccctt301 ggacatatcc tgggacggga gcccacttcc tcagagcaag gcggccttga aggatctggt361 tctgctgccg gagaaggcaa acccgctttg agtgaagagg aaagacagga acaaactaag421 aggatgttgg agctggtggc ccagaagcag cgggagcgtg aagaaagaga ggaacgggag481 gcattggaac gggaacggca gcgcaggaga caagggcaag agttgtcagc agcacgacag541 cggctacagg aagatgagat gcgccgggct gctgaggaga ggcggaggga aaaggccgag601 gagttagcag ccagacaaag agttagagaa aagatcgaga gggacaaagc agagagagcc661 aagaagtatg gtggcagtgt gggctctcag ccacccccag tggcaccaga gccaggtcct721 gttccctctt ctcccagcca ggagcctccc accaagcggg agtatgacca gtgtcgcata781 caggtcaggc tgccagatgg gacctcactg acccagacgt tccgggcccg ggaacagctg841 gcagctgtga ggctctatgt ggagctccac cgtggggagg aactaggtgg gggccaggac901 cctgtgcaat tgctcagtgg cttccccaga cgggccttct cagaagctga catggagcgg961 cctctgcagg agctgggtat ggctgcaaga ctagaaacca ggactagaaa ctgggggagt1021 agggaggcat gcctaggaaa aggagggatg caaagagaag gggctttgtg aacatggtgc1081 aaggccagga attttgggag caaaaaccaa gtatccttgt ggttcaagcc tgttttcttc1141 catcttcagg actcgtgcct tctgctgttc tcattgtggc caagaaatgt cccagctgag1201 ggcctttgtc ccattgtccc tctgtgaccc cttcatcttt gataaagcac tgacatctcc1261 ttcctaataa atagaccctg agttctgtaa aaaaaaaaaa aaaaaaa<210>13<211>1600<212>DNA<213>Homo sapiens<400>131 ggagccaccg ctgaggaaga acacacattc atcaggctgg tgaccctggt gtaccagcgc61 tttgtggcgt gtaacaaaga ctgccgagca gaaaattgga gttcaatttg ccgtccatcc121 tgcacgcagt gctcagccca ttctagtccc tggcaggcac ctctgtgctt tatcataaat181 aataacacca cgcggctcta tggccctttc cgcagttaca gtccccgagc aatttacaag241 ccagattata cacagaagcc tgcacatgcc tgccggccag cacacaccac acacacgcgg301 cctcctcccc agtcactcct agggcccacg gccatcctgg tgtcacccat ctgggcctgc361 acccaatctg gagggaagca ggccgttgtg cacccctgga cactgggctc tgctcctgcc421 ctggtcacct tctttatccc ttttactggc tctcatggtt ggcagagaac ccacgaggtc481 tggtggccca ctgccccttg ctggaccaag gtcaaggcca cttcccctct ctttctttcc541 gttttagaag gagcttctgg acctcctccc ctccaatcag cagtggccct ttgtctctac601 cacagggagt cccgtctctc ctcagaactt attgggagta gtggggggtg caaaagcagc661 aacagggacc cacaagttca accgctcgct tcacagacga gacccctggg gcccagagag721 gtgcagcagc ttccctgggg actctgcatc agctgctgag ctgagaccaa agcccaggac781 caccggcacc cagtccacta cacagcggag ccccaaggag ggaggtaaac aaaggtctag841 ggcagactgg gctgctccct ctgagcatct agaaacttcc ccagaagcag aagcttcagg901 cagggcctgg aggggctcca gccgcagctc cttccccctc ttgccatcaa actaaataag961 aggtcaacta ttgtatgatg ccatttatat gagatatcca gagtaggtag atccatagag
1021 atggggctca gattggtgac tggcaggtgg ttgggagaga ggggaatggg gagtgactgt1081 gagtgggtac aggcttcctt ctggggtgat gaaagtattc tggaactcag tggtggttgt1141 acatcattgt gaatgtacta agtgccactg aattgctcac ttgaaaatga tttttttttt1201 tttttttgag accgggtctc gctctgctgc ccaggcggga gtgtggtggt gcaatcatag1261 ctcactgcag ccttgaactc ccaggctcaa gtgatcctcc actttagcct ctcgagtacc1321 tgggaccaca ggcgcgtgcc accatgcttg gctaatattt tgtattttta gtagagatga1381 ggttttgaca cgttgcccag gctgatctcg aactcttgga ctcaagcaat gtacccacct1441 cggcctccca aagtgctagg attaaggcat gagtcagtct taaaatggct aattttatgt1501 catgcaaatc ttatccccat caaaaaaata aaaaaaaata aaaaacatac aatgggaaaa1561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa<210>14<211>1354<212>DNA<213>Homo sapiens<400>141 ggggggggac gccgagggcg gctgcgacgc gccgagaggc cgcggctctc ccacctgtca61 ccctggccct tctctgcttg gatggtgtct tcctctcctc agccgagaat gacttcgtcc121 accggatcca ggaggaactg gaccgctttc tgctgcagaa gcagctgtca aaggttcttc181 ttttcccccc actctccagt cgcctccggt acctgatcca tagaacagca gagaattttg241 atctcttgag cagcttctcc gttggggagg gctggaagag gaggacggtc atctgtcacc301 aggacatcag ggtacccagt tcggatggcc tctctggccc ctgccgcgct cctgcctcct361 gcccccgcag gtaccacggt cctcggccca tctccaacca aggagcagct gcggttcccc421 gaggtgcccg ggctggccgg tggtatcgtg gacgcaagcc tgaccagcct ttgtatgtgc481 cccgggtgct gcgcaggcag gaagaatggg ggctgacctc tacctcggtg ctcaagagag541 aggccccagc tggcagggac ccagaagagc ctggagatgt tggtgctgga gaccccaact601 ctgatcaggg actccctgtg ctgatgactc agggaacaga ggacctaaag ggcccaggac661 aaaggtgtga gaatgagcca ctgctggacc ctgttggccc tgagcctctg gggcctgaga721 gtcagtcagg gaagggagac atggtggaga tggccacacg gtttgggtcc accctgcagc781 tagacctgga aaaggggaag gagagtctgt tggagaagag gctggtggca gaggaggaag841 aggacgaaga ggaggtggaa gaggatggcc ccagcagctg ctcggaggac gattacagcg901 agctgctgca ggagatcaca gacaacctga cgaagaagga gattcagata gagaagatcc961 atttggacac atcctccttc gtggaggagc tgcctggaga gaaggacctt gcccacgtgg1021 tagagatcta tgactttgaa ccagcgctca agacggagga cctgctggca acgttttctg1081 agttccaaga gaaggggttc aggattcagt gggtggatga tacacacgca ctcggcatct1141 ttccctgcct ggcctcagct gcggaagccc tgacccggga gttctcggcg ctcaagatcc1201 ggcccctcac acagggaacc aagcagtcaa agctcaaagc cttgcagagg ccaaaactcc1261 tgcgtctggt gaaggagagg ccacagacaa atgcgactgt ggcccggcgg ctggtggccc1321 gggccctggg actccaacac aaaaaaaaaa aaaa
<210>15<211>2500<212>DNA<213>Homo sapiens<400>151 agggtcagtt tttaaaggaa aaacaactaa ctaccttgca agcttgctag caggtttaag61 tgtaataatg tgtgggaatg actgcaccgt gactaacatg cagtgacagc ttaattaatg121 ttaaccctta tcattatcat ataagaatgt gagttacata agagaggagt cctgtcagtt181 cgttctctgc tgtgtcccca agaccatgaa tcatggctgg catgtagtag gcatttaata241 atatatgttc aacaagtatt tggcagtctt ggagggcaga aaaggaggtg gggaagattt301 ttaaataaca ttttttaaaa agtcacattg tcctacaata ccgatttttc ttgcatattt361 aggaaattga gggttttttt ctaaaacatg cggacatatg ggaaatagga tgcaacattt421 gcactaatgt ttcagacaca gttagaggtt tccaagagat tttgcgctgg ggaggctgct481 tgctacaagc tcccaaagct ctgggaggac atagtattca ttcctccctc agcagaagcg541 gtgaggcaag aggctctggg gagcacccag cgttgggctt ttagcatagt gtgtcaggtc601 ttcatagttt gggcccaggg cacagagaag tcacagctct ccggcatcct gtgaccttta661 ccctctttgc caagggaaaa tgtggcccac caaagcaaga aacttgaggg catgggtcac721 cccagccctg gcatctgccc agagcccgag aaggaaggaa caatgatcct ccagctacct781 cacggggctg gcacaggtga ccactgccct ggcatcaccc agctgtgtcc ggcagcctga841 accccatctg tggggatgcg aggaggaaaa tacaaaagtc cttaggtgaa cactgagaag901 gcagatgcag cagaaacctc caggccagaa ctacccagtc ttggacctat ggtggagata961 gagcatagct ggcgatcatg tgtacttaca ctctaaggtc acctggttgc actatggcct1021 catctgtggc tctgaaaatg aagatttgga aggagatcat cacagctaat gtttaacaag1081 cccctcctgt gtgccaaatc attcacccct caccacaacc gaatgagcta aggattctca1141 ttatatatag tttatggaga gggaagtgca gacataaaga ggtgaattat cttacccaga1201 tcacacagct gataagtggt ggaggcagaa tagaatctaa acagtgtggc tccggagccc1261 acatgcattg attcgacaag tgtttattga gcacctgccg cggacaaggc cttgtgtgat1321 taaatagggt tataattagt aatataaaaa tgagaaatca ctaatgcttt ttagacttaa1381 cattttgttt ttttgtaggt ttcaggcaca gaactgtata tccaataata gtgaaatgga1441 tcccactaat tatgacagaa atgatgatac atttaaatga cttggatgtt ttataggtat1501 gatctcgtga aatcttgaga gaaactgaat gacgaatgaa actattgttc ctgtttcaca1561 cagaagaaaa ctgaggttaa aaggggtaaa gtaattttgc atggcatgaa gtagaaattc1621 aaagtacagg aatttgaact tggttctgtc cttttctgaa gcccttgacc actatagact1681 caaacatcac cttgtttttc cactcattca acactttttt ttttaaatta tctaataggt1741 tggcactcat catgagcccc tgttctcatt ctgcaaatgg tgaagctctc tattgtcctg1801 accccacagt tcctgtccca tgaccagggc cagctcacca aggagctgca gcagcatgta1861 aagtcagtga catgcccatg cgagtacctg aggaaggtga gtgagtgcag acagatgggg1921 cctggtgccc ttgagcagtt cccgggtctc agctgccaca catctcatag ccggtgatgc1981 tgggggaagc ttacgcagtc acagtactgg cttcttcctc tttttctttc catacaagtg2041 gcttagggat ggggtagagt agttgactta tttggatgaa aaccactatc ttctgtcaga2101 aactcaaaag gaatcattgc tggcatggta acctaaagaa aaacaaccag acaagtgccc2161 aacgacactt aaaaaggtga tttattatct tgccaagttt aggctgggca tggtgactcg2221 tgcctctaat cccagcattt tggggggctg aggctggtgg atcaccggag gccaggactt
2281 tgaggccagc ctgaccaata tggcaaaacc tcgtccctgc tgggggtaca aaaattagcc2341 gggcatggtg gtgtgagcct gtagtcccag ctactcagga ggctgggaca ggagaattgc2401 ttggattcgg gaggtggggg ttttagtggg ccgagatcac gccattgcac tccagactgt2461 gcgacagagc gagactctgt caaaaaaaaa aaaaaaaaaa<210>16<211>1729<212>DNA<213>Homo sapiens<400>161 ggagctgtga agctgctgga ggagttggcg tccggggagc aagggccatg gccaccgtgc61 aggagaaggc tgctgcgctg aacctctcgg ctctccacag ccccgcgcac aggcctccgg121 gtttcagtgt agctcagaag ccatttggag ccacgtatgt atggagcagc atcataaaca181 ctcttcaaac acaagtggaa gtgaaaaaac gaaggcaccg tttaaaacga cataatgact241 gctttgttgg ttcagaagct gtggatgtca ttttttctca cctaattcag aataagtatt301 ttggtgatgt agatattcct cgagccaaag tggtgagagt gtgtcaagcg cttatggact361 acaaagtatt tgaagcagtt ccaaccaaag tctttggaaa agacaaaaaa cctacatttg421 aagatagtag ttgcagcctt tatagattca ccacaatacc taaccaagac agtcagttag481 gcaaagagaa caaactatat tcacctgcca ggtatgcaga tgcattattt aagtcatccg541 atatcagatc agccagttta gaggacctgt gggaaaatct gagtttaaag cctgccaact601 cccctcatgt aaatatctct gcaaccttgt ctccacaagt tattaatgaa gtgtggcaag661 aagaaacaat tgggcgtcta ctacaacttg tagaccttcc acttcttgac tccttactga721 aacagcaaga ggctgtacct aaaattcctc aacctaagag gcagtccacc atggtcaaca781 gcagtaacta tctggatcga gggattctca aggcttatag tgactctcag gaagatgagt841 ggctctcggc agcaattgac tgttcagaat accttccaga ccaaatggtg gtggaaataa901 gcagaagctt tcctgagcaa ccagaccgaa cagacttagt gaaagaactt ctgtttgatg961 ccattggcag atattacagt agtagggaac ctctgttaaa tcacttatct gacgttcata1021 atggaattgc agaactctta gtgaatggga agacggaaat agctttagaa gctacccagc1081 tccttctaaa gcttttagat ttccaaaata gagaagaatt tagaagacta ctgtatttca1141 tggctgttgc agcaaatcct tctgagttta aattacagaa agaaagtgac aaccgaatgg1201 ttgtgaaaag gatattctca aaagctattg ttgacaataa aaatttatcc aaaggcaaaa1261 cagatcttct ggtactcttt ttaatggatc atcagaaaga tgtttttaag attcctggaa1321 ctctacataa aattgtaagt gttaagctta tggccataca gaacggaaga gatccaaata1381 gagatgcagg atatatttat tgccagagaa ttgatcaacg tgactattcc aacaatacag1441 agaagacaac caaagatgag ctgttgaatt tactaaaaac tcttgatgag gattcaaaac1501 tttctgccaa agagaagaaa aaattgctag gtcaattcta taagtgtcac ccagacatct1561 ttattgagca ttttggagac tgagttttta atatctgtat ataagttgtg tattttaaga1621 ataaattatg tatcctaaat atccaatcac atttgtaagc gtggaagctc taaatttgaa1681 actgtactta ataaaaattt ttttgtataa aaaaaaaaaa aaaaaaaaa<210>17
<211>581<212>DNA<213>Homo sapiens<400>171 agcgggccgc ccccgccccg cagggccccc aggccgggcg ctgggggcgc acaggcatac61 agattctctc tcctcaccac gcggctgctg ctggaggatg gaggaggggt ggtgtcagca121 attcaagact gtctttccta ccctcttcag tgcccttcct tgataagctg tttaaaccag181 aaccgactgt ttctggaaaa ggtaaacatc tattctccaa agggcagtga ctttctacct241 gtgaaaataa aaagtaaact tcagaagaaa attctgaaag aggaatttag agatgttcca301 aataaaggca atatagtttc actggaactg aaatgttgcc ttccaattct actacagaag361 gcagagtggc acatgtggca cctggtcagg atggactcac taaaggattt aacccagact421 tctgatcaac aaatattgct gctacattaa ctgattcgca acaaagaaca gaaaatatca481 tccagttgat tttacttatt tacatatggg ctcaaatctt aaatctgaca aactaataaa541 taaatatgtc tgaaaacaaa aaaaaaaaac tctccagcgc t<210>18<211>764<212>DNA<213>Homo sapiens<400>181 gtcacttgag cagaggagtt caaggccaac ttgggcaaca tagtgagacc ccgtctctaa61 gacaaataat aaacatcaaa attaaaatta aaaaaaaccc aacccagtgt tcccttcaga121 atcctgagtg gtttgtttca tcttcctcta ttctggcttg tgtcatcttc ctctcttgta181 atggataaaa actccaaatg aagacaaaaa tgaggaattt gtcaaaaaaa ttgaattgcc241 aacaaagcca tctttggttg atcaaacggt acagacgttt cagttagaca agagggctaa301 gtttctgaga cccattgtac aggattatta atattattca tttttagccc tcataataag361 gtattataaa tgtcaagatt gctaaaagaa tacgttttta aatgtcttca ttataaaaaa421 taagtatgtg aggtgatgga catattaatc agcttaatat aatcatttta tagtatatgt481 agctatatca aaacatcaca tttataaccc ataactacat gcaattatta tttgtcaatt541 aaaaatacaa atctaggcca aggtgggagg atcccttgag cccaggagtt tgacagcagc601 ttgggcaaca tagggagact ccatctctac aaaaaaaaaa aaacccgcac cacaccgcgc661 acccacaccg gggggggaaa ccgccaaaca cttgcccggg cgcggggggg ggcccctgcc721 cggaatccca acacggtccg ggaaacccga gggggggagg agcc<210>19<211>504<212>DNA<213>Homo sapiens<400>19
1 tcataccaac tatggcaatt tttatttcat ttgccctgta gccatgtcat ataccaggtt61 tttaaaaaat agggaaataa ggaatttact cactttgact tcagattcac tttttcaaat121 gtcactcaca tttttttcac atagaagata ttcaaaagac aaaggattta tttacccttt181 ttgcaacaga aaattacaca attaaaaatt cctatgtcca gtgtcactga atggggaaag241 aggcaatata ttttaaaaaa caagaggaaa aaatgctgag gaaaaaaatg tgcaacaaat301 ctaaattctt ctaatcttga attcttatat acaactagaa aaggtaatgg caatatagat361 ggtctctgat gctcagaaac acatttcatc actttctttt ctgcttcatc cagacatttt421 cagtactgtc aagctcaaaa acacagtttt agaaagatga taaataagag aatctcaaag481 acttcacatg agctcttctg atca<210>20<211>622<212>DNA<213>Homo sapiens<400>201 ccaacactta gggaggctga ggtgggcaga tcacctgagg ttgggagttc gagaccagcc61 tgaccaacat ggagaaatgc catctctact aaaaatacaa aaattagccg ggcatggtgg121 cacgtgcctg taatcccagc tactcaggag gctgaggcag gagaatcgct tgaacctggg181 aggtggaagt tgcagtgagc tgagatcacg ccattgcact ccagcctggg caacaagcga241 aactctgtct caaaaaaaaa agaaaagaca gtagcatatg ttcatgtcaa gcacctctca301 tcacagtcga gttccaagga aaaattccag cgttttctgc attgggtgct gtgtcatctg361 aaatcggcac attccatgga ggaacggagt cctgctttgt tgcatgtatc ctagggttta421 atgttggtaa atgagtcact ctagcatttg tagaaggctc cctgagactc ctgcagcagt481 cgaccaagcc caaggacata attgaatctg gagagtcctg gggccttgtt tggacacaga541 cttgaaatac acataggaag aaaggcataa aaatacatgt tcacttgtct ctgctgtgaa601 caacagaaaa accacacaag ag<210>21<211>759<212>DNA<213>Homo sapiens<400>211 cgcggtggct cacgcctgta atcctatcct ttaggaggcc gaggtgggcg gattgcctga61 gttcaggagt tcgagaccag cctgggcgac acggtgaaac cccgtcccta ctaaaataca121 aaaaattagc cgggcgtggc ggcacgcgcc tgtaatccca gctactcagg aggctgaggc181 aggagaatgg cttgaactcg ggtgggggag gttgcaatga gccgagatcg cgccgctgca241 ctccagcctg ggcgacagag cgagactctg tctctgaaaa aaaacaaaac gaaacgagaa301 ggctttcaaa attactctgc ggtacactaa tttaacagat tagatcactt tccaggcttt361 aagttggcct ttgttctacc ctttttttta gtgtatgata aaagttgtgt ggttcgttga421 tcagcatgca atgaactctg gctcttgtta atcaggtgac tacagttttg tccttacatt
481 tgtatgtatg tcatgagtgg tttgtatggg caggaggtaa acaacgcaca tttaaatata541 cttaatgatt tatcattcca acgttttctg gtggtttagt ctttcattgt gtctcttgaa601 caatctgaaa catccctatt ctaaggctaa aaaaatttcc tttacaaact taggttaaac661 attcctaatc tgaaatccaa aaatgctcta aaattcgaat tttttgagtg ctgacgtgac721 ccacaaatgg aaaatccaca ttgccctcat gtgacacgg<210>22<211>870<212>DNA<213>Homo sapiens<400>221 ggcatccata gactatttcc ttgcttacaa gctcagtgaa catactgaat tcacttataa61 aaagaatttt ctcaatttta gttgcttttc aaaaaattct tattcctttt atttgtaatt121 ctttgacatt gttgctgctt cacagttttg gtaattggac agtttggtct ttattatgat181 attaacattt ttacctgtcc tgaaacaagg aaatacaatc aaagaaacag aagattggtg241 gaccttgaac cacattatga tatcagatga cccagagtca tggttactaa gttgcttaaa301 tttttagtaa caaagatgta ttagaaagtg acaggtcaat tcaataatgg tttttttgga361 tacatttaat tatataatcc ccttaagttg tgcttagtca gcagtattta atctgttgtc421 tattatatat ctaactggtc tgttatgttt attatatatt tatatattgc ttattcatat481 cttatggagt aactcatttt taaaacaata tatttcatct cctaggagaa gaggatcgag541 tatgtgttta ttcttttcat taggacatgg ggtaattcta ttgtgaaatg tgttggcata601 tttccattca ctcggcttgg gaactttgaa cctttaattc cgtagcacat ggatttaatt661 agtgtcatcc tttactgaat acatagagat aaagagaccg tataatgact acttcccatt721 catgagaatg ccaccaaagg ttgggtatca aactctggtt gtcactttag acaataaaag781 gaatcttcta atattggaga caataattcc tcaaaaaatt cgccttttca ctttctcttt841 cttcatatcc gacattttat atatgnnngt<210>23<211>882<212>DNA<213>Homo sapiens<400>231 caatcttcat caatctgttc atcataagac agagtaagaa tttttaacag aaaattctaa61 aagagataga agctaggaaa gcttctttat atcttgcatt aaatgggtag ggaaaaaata121 tgactctcac ccatgtcatg tactggacca tctttctcta ctttttaaag aaaaactaat181 acaactagaa aaacatcaca acaagccaaa taaaaatgga gaaaaggagt catgtcctta241 tacctaatgg aacagtttcc agaactactt taactatgga agcatttttg aaaataaagt301 attaataaaa tagaaaaaaa ttattaaata tattgtatcc ttaatgtata gcaggaaaat
361 agtttcacaa attaatgcta ttagaaatta ccaaattttt attaagtact ttaatatgca421 atacaaattg aataatagta caactataag ttgatataaa gtaagtgctt actggtaatc481 attaaataac taataacatg acttgttggt taatgttaaa cctacagtaa gaaatgttat541 acagatcaga taggcattag aacatcaggt gatacctttt ctgatttctg gcattcatgg601 acaccatttc ttaaaagcag attgttgtat tataacatat accatgtaat tagggttgtt661 gcaacaatta tatttgcaaa tttgtagaag aaactagcct aaatccaggt gaatgtgttc721 atatgggtat taattcctga aacatggagt ttttgagatg gcgaattgta aaggggttat781 agaagatcct taaccttacc tggggaagga caaattggct ctgagcagac attagggaaa841 aggagataaa tgtcccgcaa gaaaacggat aaaaaaaaaa gg<210>24<211>668<212>DNA<213>Homo sapiens<400>241 gcctggccgg cgcccccgcg cccaaggcct acgtgcgggg gcagcccagc cctggccgcg61 gagctggagg ccgtgggcgt cgccagcgtg ggcgtggggc ccgagccact gcagggcgag121 ggtcccggcg actggctgca cgcgccgctg gagcccgacg tgcgcgcggt ggtggtgggc181 tttgacccgc acttcagcta catgaagctc accaaggccc tgcgctacct gcagcagccc241 ggctgcctgc tcgtgggcac caacatggac aaccggcttc cgcttgagaa cggccgcttc301 atcgcgggta ccgggtgtct ggtccgagcc gtggagatgg ccgccagcgc caggccgaca361 tcatcgggaa gcccagccgc ttcattttcg actgcgtgtc ccaggaatac ggcatcaacc421 ccgagcgcac cgtcatggtg ggagaccgcc tggacacaga catcctccta ggcgccacct481 gtggcctgaa gaccatcctg accctcaccg gagtctccac tctaagggat gtgaagaata541 atcaggaaag tgactgcgtg tctaagaaga aaatggtccc tgacttctat gttgacagca601 tagccgacct ttgcctgcct tcaaggttaa agattgagtg tctttaatct gcagaataaa661 caaaaaaa<210>25<211>238<212>DNA<213>Homo sapiens<400>251 agagtgcgcg caagatattg ttggcgtgaa aatatcggcc gcaaactccc cgctgaggcc61 aacgaggcca cgcttttatt tgggctttaa aattagctgc aaatggatgt ggagttttct121 ctgggtcttg cgaggtgttt cactcttctt ccaagccatg taactcaatc ttgctcctcc181 ctggtgtttg caggtggcaa gagctgtgct ggatgttgag gccttgctcg aagacact
<210>26<211>633<212>DNA<213>Homo sapiens<400>261 gaccatgcgc ggcgcggggg cgatcctgcg gccgggggcg cgtggtgccc gggacctgaa61 cccgcggcgg gacatctcct cctggctggc ccagtggttc cctagaaccc cagccaggtc121 cgtggtggcc ctgaagaccc ccatcaaggt ggagctggtg gcagggaaaa cctacaggtg181 gtgtgtgtgt ggccgcagca agaagcagcc cttctgtgac ggctcccact tcttccaacg241 cactggccta tctccactca agttcaaggc ccaagagacc cgcatggtgg cactctgtac301 ctgcaaggcc actcagaggc ccccgtactg cgatggcacc cacaggagtg agcgcgtgca361 gaaggcagaa gtgggctccc cactctgagg gggctgctgc tgtccagcca caggtggcct421 tggctccagg cctctgacag gcaccccctt ctgtgggaaa ggaaacaggt gctgagccca481 agagactctg gtacccactg ctggctcatg aaggaagaat tattccttat aacctaaaag541 tctccagtct ggggcaggcg ggagtgggcc ctggttcaat gtttgctgat ggggaagatg601 gcaaaacaag cctgcccaac agaaaaaaaa aca<210>27<211>640<212>DNA<213>Homo sapiens<400>271 gaaaaacagc cattttcaac ctttttataa aacctcttca gggaatattg gaatattatt61 ttgtcaggca aaaataatcc aggcttgagc attgctgtag aatatgttat ctgttcaaaa121 cttagcagag cacgcatgca tcacatgctt tgtcatcagt atttgagagc tgaaccaatg181 gtttttgatg gttgccgtaa cagaagatct gtgaagagga tggtggtttg cacctgttcc241 tgaagtcaaa ctggaaaagg ttgcaggtat ttcaattttc tcaatcattt tgcataaatg301 tattaatatt ttgagtagta atagttctaa acctgcagga aggcatcatt gtatactgtg361 gtatgtagag aatactactt ttacactgct ctttatctta aaatgttttg gtatactgtg421 aatattttcc tctaagtatt tggtgtattt tgacagttat taaaggagaa tataaattaa481 agattctggt ctaaactaag aataatggaa agttgcttcg tcctgcctta gaggagtttt541 cagttcatcc tcttcttgag aaactatgtg tgctgttact aaataaacat ttactcaata601 aacgttttag tttccttgaa aaaaaaaaaa aaaaaaaaaa<210>28<211>505<212>DNA<213>Homo sapiens
<400>281 ggcgaggacc cgggtctggc gctgtgggcc gggagccgtg gggcggcatg gaggggctgg61 ttgtcgccgc cggcggggac gtctccctgc acaacttcag cgcgaggctg tgggagcagc121 tggtccactt ccacgtcatg cggctgacgg actcgctgtt cctgtgggtg ggggccacgc181 cgcacctgcg caacctcgcc gtggccatgt gcagccgcta cgactccatc cccgtgtcta241 cctccctcct tggagacact tccgacacga cctctactgg ccttgcccag cgcctagcca301 ggaagaccaa caaacaggtg tttgtcagct ataaccttca gaacacagac agtaacttcg361 cattacttgt agaaaacagg atcaaggaag agatggaggc tttccccgaa aagttctagc421 tgagtggcag aagtgagaat ttgtaaactt atgtacaatg tacgtgtaaa taaatggatt481 gaatttcaaa accacaaaaa aaaac<210>29<211>542<212>DNA<213>Homo sapiens<400>291 acacacaatg gaatattatt tagtcttaaa agggaaggaa attctgacac atgctacaag61 atggataaac cacaaggaga ttatgctatg tgaaatatgc cagtcacaaa aatacaaata121 ctgtatgata ccacttacac ggggtacaca gtcaaattca cagagacaga actagaatgg181 aggttaccaa ggctagctgg aaaatggcga atgagaaatt gtttaatggg ttcggggttt241 gttttgcaag atgaaaagaa ttctggagac tggccgcacg aggtaaatat acttaatact301 aacgaattat tcactcaaaa atggttaaga taataaattt tgttgtgtag ataaaaaaat361 ttaaaaatcg attccatctt tctcatcaaa tcttactttt tactctttgt ttcttttttt421 cccatttcag ttagattgct ctcttcattg tacttccagt atgcctcttg agcctttgca481 ccatggtgtt tcttctctgg aatggtcttt cattatcacc catccatttt caaaaggttg541 ca<210>30<211>572<212>DNA<213>Homo sapiens<400>301 agactgaatt gaatgtcttt aatttcacac aatgaataac atatgaatag gattaacttt61 ttttttttaa tagttaagtg ctctatactc gtgcctaacc tgatcatggt agacggaaaa121 gacatgagac tttcttttag aaanaaaatg tttaaaaccc aacatctaag accatggagg181 agcatgacag taagcatgct agatgtatct acgcctcaaa taacagcaag tccatgttca241 ttttaaatgt acaaaaatgc tttatgaata aaaactgtta caaaaagaac atttcaaaca
301 atgtacaatt ttcttgcctc tatattcaat aaaatacaaa tacatttttt gttctaaaat361 atgtttacat acaatcaaag tagaacatgc aaattacact ttaaaaaagg cttttaaaaa421 ccacttatga atacaaacta aaaattagcc agcacgactt gtaagacaat cttgtgcccc481 attttttctt ttgaaatata tatgtacata tttaacacat catgtgcatt tattattatt541 taagaaatag atttttttta aaaaaaggaa ga
權(quán)利要求
1.一類(lèi)人類(lèi)肝臟相關(guān)基因，其包括以下序列的基因(a)SEQ ID No.1～SEQ ID No.30所示的序列；(b)SEQ ID No.1～SEQ ID No.30所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.30所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數(shù)條的組合。
2.根據(jù)權(quán)利要求1所述的一類(lèi)人類(lèi)肝臟相關(guān)基因，其特征在于所述人類(lèi)肝臟相關(guān)基因包括具有SEQ ID No.1～SEQ ID No.30所示序列的基因。
3.一種探針?lè)肿?，其特征在于所述的探針?lè)肿雍袡?quán)利要求1中所述的序列中約8-100個(gè)連續(xù)的核苷酸。
全文摘要
本發(fā)明公開(kāi)了一類(lèi)人類(lèi)肝臟相關(guān)基因。本發(fā)明的人類(lèi)肝臟相關(guān)基因與一些肝臟疾病相關(guān)，在研究肝臟疾病的致病機(jī)理以及開(kāi)發(fā)治療肝臟疾病的藥物中發(fā)揮重要作用。
文檔編號(hào)C12Q1/68GK1970755SQ200510110589
公開(kāi)日2007年5月30日 申請(qǐng)日期2005年11月22日 優(yōu)先權(quán)日2005年11月22日
發(fā)明者黃健, 韓澤廣 申請(qǐng)人:上海人類(lèi)基因組研究中心