專利名稱：負(fù)責(zé)fr－008聚酮抗生素生物合成的基因簇的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及的是一種聚酮抗生素及其生物合成基因，特別是一種鏈霉菌FR-008中多烯聚酮合酶基因簇，屬于生物技術(shù)基因領(lǐng)域。
背景技術(shù)：
抗生素是由微生物、植物和動(dòng)物等產(chǎn)生的，能夠在很低濃度下就可以殺死或抑制其它生物生長(zhǎng)的次級(jí)代謝產(chǎn)物。如微生物所產(chǎn)生的青霉素、作為抗癌藥物的紫杉醇(taxol)等。抗生素通過干擾靶細(xì)胞的一個(gè)或多個(gè)生理代謝過程而發(fā)揮作用。聚酮是一類自然產(chǎn)物，很多聚酮類抗生素已應(yīng)用于醫(yī)用，獸用及農(nóng)用，包括紅霉素(抗菌)，制霉菌素(抗真菌)，阿維菌素(抗寄生的)，納巴霉素(免疫抑制劑)和道諾紅菌素(抗腫瘤)。革蘭氏陽性菌鏈霉菌是聚酮抗生素的主要產(chǎn)生菌。聚酮的合成與脂肪酸的合成類似，都是經(jīng)由聚酮合酶介導(dǎo)的簡(jiǎn)單羧酸單位的縮合過程，而且與聚酮抗生素合成相關(guān)的合成基因，調(diào)節(jié)基因及抗性基因在細(xì)菌基因組中往往緊密連鎖，成簇排列。負(fù)責(zé)紅霉素大環(huán)內(nèi)酯形成的DEBS聚酮合酶是第一例被發(fā)現(xiàn)具有模塊結(jié)構(gòu)。隨著越來越多大環(huán)內(nèi)酯聚酮的分離與鑒定，它的模塊化核心概念始終沒有發(fā)生改變。I型聚酮合酶的模塊結(jié)構(gòu)包括重復(fù)的酮基合成酶結(jié)構(gòu)域(KS)，酰基轉(zhuǎn)移酶結(jié)構(gòu)域(AT)，酰基載體蛋白結(jié)構(gòu)域(ACP)(也可能包含酮基還原酶結(jié)構(gòu)域(KR)，脫氫酶結(jié)構(gòu)域(DH)，烯酰還原酶結(jié)構(gòu)域(ER))，每個(gè)模塊負(fù)責(zé)聚酮鏈延伸的一次反應(yīng)，它們決定著加入的延伸單位的還原步驟。此外，還需要硫酯酶(TE)結(jié)構(gòu)域的作用催化聚酮鏈的環(huán)化與釋放。最后，還要經(jīng)過糖基化、羥基化、甲基化和?；刃揎棽襟E。例如免疫抑制劑FK506通過抑制T細(xì)胞激活與生長(zhǎng)所需的信號(hào)傳導(dǎo)途徑來阻礙免疫應(yīng)答。FK506被用來防止移植排拒反應(yīng)。并被用于某些自動(dòng)免疫疾病的治療。在結(jié)構(gòu)上，F(xiàn)K506也是一個(gè)大環(huán)內(nèi)酯，可由一些鏈霉菌產(chǎn)生。
聚酮生物合成PKS的模塊結(jié)構(gòu)組成具有一定的可塑性，使得人們能夠通過改變模塊的數(shù)目、延伸模塊的特異性或結(jié)構(gòu)域的插入或失活等基因工程操作獲得新的聚酮衍生物。
天然大環(huán)內(nèi)酯抗生素多樣性的一個(gè)重要資源是中心配基特定位點(diǎn)上所結(jié)合的糖基。這些糖基組分被發(fā)現(xiàn)幾乎均與抗生素和其細(xì)胞內(nèi)作用對(duì)象之間分子識(shí)別有關(guān)。許多天然存在的生物活性代謝物攜帶有不尋常的糖基，作為生物活性分子識(shí)別的要素。近來，對(duì)于糖苷轉(zhuǎn)移酶及其它糖基合成基因的深入了解越來越多地被用來產(chǎn)生新型糖基雜合的抗生素。糖苷轉(zhuǎn)移酶對(duì)底物的一定適應(yīng)性使糖苷轉(zhuǎn)移酶成為組合生物合成的引人注目的工具。
氨基海藻糖基團(tuán)存在于很多大環(huán)內(nèi)酯抗生素分子中，大部分多烯抗生素分子攜帶這種特殊的氨基海藻糖基團(tuán)。預(yù)測(cè)的氨基海藻糖合成途徑包括來自主代謝中的GDP-甘露糖在GDP-甘露糖脫水酶的作用下轉(zhuǎn)化為GDP-4-酮基-6-脫氧-D-甘露糖，然后異構(gòu)化為GDP-3-酮基-6-脫氧-D-甘露糖，最后通過GDP-酮糖轉(zhuǎn)氨酶的轉(zhuǎn)氨作用形成GDP-氨基海藻糖。其中異構(gòu)化可能并不需要酶的參與。
天然抗生素的另一個(gè)重要特點(diǎn)是已合成好的中心配基特定位點(diǎn)上可經(jīng)過氧化酶的后修飾而獲得很大范圍的結(jié)構(gòu)特征。這些氧化酶介導(dǎo)的后修飾包括羧基化，羥基化，環(huán)氧化等。大多數(shù)情況下這些后修飾步驟導(dǎo)致產(chǎn)生藥效團(tuán)特性。因而這些后修飾氧化酶基因具有很大的化學(xué)和藥理學(xué)重要性。在組合生物合成中，這些后修飾的氧化酶基因扮演著重要的角色。考慮到聚酮化合物后修飾蛋白在生物活性及結(jié)構(gòu)多樣性方面的重要作用，更加廣泛的后修飾氧化酶基因可以得到應(yīng)用并擴(kuò)大可應(yīng)用后修飾酶的工具箱。近來，對(duì)于后修飾氧化酶基因的深入了解越來越多地被用來產(chǎn)生新型的雜合的抗生素。后修飾氧化酶基因?qū)Φ孜锏囊欢ㄟm應(yīng)性也使其成為組合生物合成的引人注目的工具。
聚酮細(xì)胞色素P450單氧化酶催化前體聚酮位點(diǎn)特異性氧化而得到最終的大環(huán)內(nèi)酯抗生素，包括紅霉素，泰樂菌素，竹桃霉素，阿維菌素等。這些聚酮細(xì)胞色素P450單氧化酶的作用底物以及在相同底物的作用位點(diǎn)都有很大的不同。底物結(jié)構(gòu)的微小變化就會(huì)給這些氧化酶作用活性造成很大影響。
另一方面，一直以來，提高產(chǎn)量是抗生素工業(yè)生產(chǎn)致力于解決的優(yōu)先問題。然而，傳統(tǒng)的通過改善發(fā)酵條件，或通過化學(xué)誘變篩選產(chǎn)量提高的抗生素產(chǎn)生菌越來越受到限制，并且很容易達(dá)到飽和。隨著生物技術(shù)的發(fā)展特別是基因工程的飛速發(fā)展，提供了通過改造抗生素產(chǎn)生菌中相關(guān)基因而大幅度提高抗生素產(chǎn)量的途徑。這其中增加正調(diào)節(jié)基因拷貝數(shù)或打斷負(fù)調(diào)節(jié)基因是最重要的途徑之一。
多烯大環(huán)內(nèi)酯是一類在大環(huán)內(nèi)酯中包含3-8個(gè)連續(xù)雙鍵的聚酮化合物。多烯抗生素通過與真核細(xì)胞膜的固醇相互作用形成通道而引起小分子的丟失而導(dǎo)致細(xì)胞死亡。
多烯大環(huán)內(nèi)酯類抗生素兩性霉素B是結(jié)核鏈霉菌產(chǎn)生的一種在醫(yī)藥上具有重要用途的抗真菌抗生素。兩性霉素B對(duì)真菌細(xì)胞膜中數(shù)量最大的固醇麥角固醇具有很大的親和性，從而對(duì)真菌細(xì)胞具有選擇性的毒性。然而它也會(huì)與哺乳動(dòng)物細(xì)胞膜中的膽固醇發(fā)生作用而具有毒性，尤其是腎毒性。盡管如此，兩性霉素B仍然是人類嚴(yán)重系統(tǒng)性真菌感染最重要的抗生素。兩性霉素B還可使培養(yǎng)細(xì)胞免受艾滋病毒(HIV)的感染。多烯大環(huán)內(nèi)酯類抗生素制霉菌素也是人類真菌感染重要的醫(yī)用抗生素。殺念菌素D是由灰色鏈霉菌IMRU3570產(chǎn)生的一種芳香七烯大環(huán)內(nèi)酯抗生素。它對(duì)念珠菌有很強(qiáng)的抑制活性。

發(fā)明內(nèi)容
本發(fā)明根據(jù)背景技術(shù)中存在的不足和需要解決的技術(shù)問題，提供一種鏈霉菌FR-008中FR-008生物合成基因簇，其產(chǎn)生的一種多烯聚酮抗生素FR-008具有抗真菌活性和對(duì)蚊子幼蟲的高毒性，而且可以防止前列腺腫大以及可以防止陰道滴蟲感染和念珠菌陰道炎。
本發(fā)明提供一種鏈霉菌FR-008中多烯聚酮FR-008生物合成基因簇，即包含fscA，fscB，fscC，fscD，fscE，fscF，fscTI，fscTII，fscRI，fscRII，fscRIII，fscRIV，fscP，fscFE，fscTE，fscMI，fscMII，fscMIII，fscO，pabAB，pabC這一系列共21個(gè)基因及其DNA序列，即序列1，并提供了這些基因及其DNA序列所編碼FR-008生物合成酶的氨基酸序列，即序列2-22；包括包含序列1中堿基20,927-26,158的基因fscA，包含序列1中堿基60,860-28,983的基因fscC，包含序列1中堿基77,587-60,962的基因fscB，包含序列1中堿基84,132-77,983的基因fscF，包含序列1中堿基107,485-84,170的基因fscE，包含序列1中堿基136,148-107,496的基因fscD的核苷酸序列，編碼了聚酮合酶；包括包含序列1中堿基26,333-27,340的基因fscTI，包含序列1中堿基27,561-28,280的基因fscTII，包含序列1中堿基3,818-3,150的基因fscRI，包含序列1中堿基7,205-4,377的基因fscRII，包含序列1中堿基10,320-7,210的基因fscRIII，包含序列1中堿基13,315-10,298的基因fscRIV，包含序列1中堿基16,106-17,287的基因fscP，包含序列1中堿基17,334-17,528的基因fscFE，包含序列1中堿基17,556-18,413的基因fscTE，包含序列1中堿基13,522-14,898的基因fscMI，包含序列1中堿基14,953-16,011的基因fscMII，包含序列1中堿基137,766-136,558的基因fscMIII，包含序列1中堿基1,950-574的基因fscO，包含序列1中堿基18,610-20,781的基因pabAB，包含序列1中堿基3,037-2,264的基因pabC的核苷酸序列，編碼了ABC轉(zhuǎn)運(yùn)蛋白，調(diào)節(jié)蛋白，細(xì)胞色素P450單氧化酶，鐵氧化還原蛋白，II型硫脂酶，糖基合成酶，糖苷轉(zhuǎn)移酶，依賴FAD的單氧化酶，對(duì)氨基苯甲酸合成酶。共有負(fù)責(zé)FR-008生物合成的6個(gè)聚酮合酶基因，4個(gè)調(diào)節(jié)基因，2個(gè)轉(zhuǎn)運(yùn)基因，2個(gè)對(duì)氨基苯甲酸合成基因，2個(gè)糖基合成酶，1個(gè)糖苷轉(zhuǎn)移酶基因和4個(gè)修飾基因獲得確認(rèn)，并提供這些基因所編碼酶的氨基酸序列。
本發(fā)明是根據(jù)如下技術(shù)方案實(shí)現(xiàn)的，本發(fā)明提供了一種來自于鏈霉菌FR-008中編碼了聚酮合酶，ABC轉(zhuǎn)運(yùn)蛋白，調(diào)節(jié)蛋白，細(xì)胞色素P450單氧化酶，鐵氧化還原蛋白，II型硫脂酶，糖基合成酶，糖苷轉(zhuǎn)移酶，依賴FAD的單氧化酶，對(duì)氨基苯甲酸合成酶的抗生素FR-008基因簇，并提供了這些核苷酸序列所編碼FR-008生物合成酶的氨基酸序列，也就是說這些酶由序列2-22提供的氨基酸序列來定義，這些核苷酸序列是分別選自于序列1中包括fscA(20,927-26,158)，fscC(60,860-28,983)，fscB(77,587-60,962)，fscF(84,132-77,983)，fscE(107,485-84,170)，fscD(136,148-107,496)，fscTI(26,333-27,340)，fscTII(27,561-28,280)，fscRI(3,818-3,150)，fscRII(7,205-4,377)，fscRIII(10,320-7,210)，fscRIV(13,315-10,298)，fscP(16,106-17,287)，fscFE(17,334-17,528)，fscTE(17,556-18,413)，fscMI(13,522-14,898)，fscMII(14,953-16,011)，fscMIII(137,766-136,558)，fscO(1,950-574)，pabAB(18,610-20,781)，pabC(3,037-2,264)的一系列基因序列。
包括包含序列1中堿基20,927-26,158的基因fscA，包含序列1中堿基60,860-28,983的基因fscC，包含序列1中堿基77,587-60,962的基因fscB，包含序列1中堿基84,132-77,983的基因fscF，包含序列1中堿基107,485-84,170的基因fscE，包含序列1中堿基136,148-107,496的基因fscD的聚酮合酶基因編碼了包含有聚酮合酶模塊的酮基合成酶結(jié)構(gòu)域即KS結(jié)構(gòu)域，?；D(zhuǎn)移酶結(jié)構(gòu)域即AT結(jié)構(gòu)域，?；d體蛋白結(jié)構(gòu)域即ACP結(jié)構(gòu)域，也可能包含酮基還原酶結(jié)構(gòu)域即KR結(jié)構(gòu)域，脫氫酶結(jié)構(gòu)域即DH結(jié)構(gòu)域，烯酰還原酶結(jié)構(gòu)域即ER結(jié)構(gòu)域的聚酮合酶。
由序列2-22定義的FR-008生物合成酶對(duì)應(yīng)于FR-008生物合成的每一個(gè)合成步驟和后修飾過程以及調(diào)節(jié)，轉(zhuǎn)運(yùn)，矯正過程。
本發(fā)明還提供了編碼包括ATP依賴性羧酸輔酶A連接酶(CoL)，ACPL，KS1，AT1，ACP1的FR-008聚酮合酶結(jié)構(gòu)域的DNA分子的核苷酸序列，這些結(jié)構(gòu)域分別通過序列2中氨基酸51-465，581-647，661-1,094，1,202-1,509，1,596-1,662來描述，命名為fscA基因的核苷酸序列選自于序列1中的包括20,927-26,158的一系列堿基。
本發(fā)明還提供了編碼包括KS10，AT10，DH10，KR10，ACP10，KS9，AT9，DH9，KR9，ACP9，KS8，AT8，DH8，KR8，ACP8，KS7，AT7，DH7，KR7，ACP7，KS6，AT6，DH6，KR6，ACP6，KS5，AT5，DH5，KR5，ACP5的FR-008聚酮合酶結(jié)構(gòu)域的DNA分子的核苷酸序列，這些結(jié)構(gòu)域分別通過序列3中氨基酸8,808-9,221；9,330-9,627；9,680-9,867；10,183-10,366；10,469-10,536；6,966-7,383；7,490-7,792；7,845-8,029；8,331-8,514；8,618-8,685；5,253-5,675；5,775-6,074；6,127-6,310；6,590-6,773；6,874-6,941；3,529-3,954；4,059-4,356；4,407-4,595；4,884-5,063；5,163-5,230；1,787-2,212；2,318-2,617；2,670-2,859；3,155-3,338；3,438-3,505；34-460；576-875；928-1,112；1,417-1,596；1,696-1,763來描述，命名為fscC基因的核苷酸序列選自于序列1中的包括60,860-28,983的一系列堿基。
本發(fā)明還提供了編碼包括KS4，AT4，DH4，KR4，ACP4，KS3，AT3，DH3，ER3，KR3，ACP3，KS2，AT2，KR2，ACP2的FR-008聚酮合酶結(jié)構(gòu)域的DNA分子的核苷酸序列，這些結(jié)構(gòu)域分別通過序列4中氨基酸3,703-4,103；4,247-4,550；4,600-4,722；5,103-5,286；5,389-5,456；1,597-2,023；2,139-2,442；2,494-2,684；2,970-3,322；3,325-3,508；3,610-3,677；33-460；571-874；1,224-1,406；1,508-1,574來描述，命名為fscB基因的核苷酸序列選自于序列1中的包括77,587-60,962的一系列堿基。
本發(fā)明還提供了編碼包括KS21，AT21，DH21，KR21，ACP21，TE21的FR-008聚酮合酶結(jié)構(gòu)域的DNA分子的核苷酸序列，這些結(jié)構(gòu)域分別通過序列5中氨基酸33-456；545-851；903-1,083；1,378-1,561；1,668-1,735；1,814-2,018來描述，命名為fscF基因的核苷酸序列選自于序列1中的包括84,132-77,983的一系列堿基。
本發(fā)明還提供了編碼包括KS20，AT20，DH20，ER20，KR20，ACP20，KS19，AT19，KR19，ACP19，KS18，AT18，DH18，ER18，KR18，ACP18，KS17，AT17，DH17，ER17，KR17，ACP17的FR-008聚酮合酶結(jié)構(gòu)域的DNA分子的核苷酸序列，這些結(jié)構(gòu)域分別通過序列6中氨基酸5,642-6,069；6,158-6,463；6,515-6,693；7,017-7,322；7,330-7,512；7,615-7,682；4,117-4,544；4,633-4,936；5,263-5,445；5,552-5,619；2,071-2,498；2,590-2,896；2,948-3,127；3,428-3,733；3,741-3,924；4,025-4,092；34-460；548-850；902-1,081；1,384-1,689；1,697-1,880；1,982-2,049來描述，命名為fscE基因的核苷酸序列選自于序列1中的包括107,485-84,170的一系列堿基。
本發(fā)明還提供了編碼包括KS16，AT16，KR16，ACP16，KS15，AT15，KR15，ACP15，KS14，AT14，KR14，ACP14，KS13，AT13，KR13，ACP13，KS12，AT12，KR12，ACP12，KS11，AT11，DH11，KR11，ACP11的FR-008聚酮合酶結(jié)構(gòu)域的DNA分子的核苷酸序列，這些結(jié)構(gòu)域分別通過序列7中氨基酸7,935-8,352；8,464-8,767；9,099-9,280；9,387-9,452；6,425-6,845；6,935-7,241；7,559-7,725；7,838-7,898；4,872-5,297；5,408-5,720；6,049-6,225；6,331-6,398；3,342-3,765；3,872-4,170；4,499-4,676；4,782-4,849；1,788-2,209；2,313-2,621；2,972-3,150；3,254-3,314；38-452；563-864；921-1,105；1,410-1,592；1,694-1,760來描述，命名為fscD基因的核苷酸序列選自于序列1中的包括136,148-107,496的一系列堿基。
本發(fā)明還提供了編碼2個(gè)ABC轉(zhuǎn)運(yùn)蛋白的基因的核苷酸序列，這兩個(gè)ABC轉(zhuǎn)運(yùn)蛋白分別由序列8和序列9的氨基酸序列組成，命名為fscTI，fscTII基因的核苷酸序列選自于序列1中包括26,333-27,340，27,561-28,280的一系列堿基。
本發(fā)明還提供了編碼4個(gè)調(diào)節(jié)蛋白的基因的核苷酸序列，這4個(gè)調(diào)節(jié)蛋白分別由序列10，11，12，13的氨基酸序列組成，命名為fscRI，fscRII，fscRIII，fscRIV基因的核苷酸序列選自于序列1中包括3,818-3,150，7,205-4,377，10,320-7,210，13,315-10,298的一系列堿基。
本發(fā)明還提供了編碼一個(gè)細(xì)胞色素P450單氧化酶的基因的核苷酸序列，這個(gè)細(xì)胞色素P450單氧化酶由序列14的氨基酸序列組成，命名為fscP基因的核苷酸序列選自于序列1中包括16,106-17,287的一系列堿基。
本發(fā)明還提供了編碼一個(gè)鐵氧化還原蛋白的基因的核苷酸序列，這個(gè)鐵氧化還原蛋白由序列15的氨基酸序列組成，命名為fscFE基因的核苷酸序列選自于序列1中包括17,334-17,528的一系列堿基。
本發(fā)明還提供了編碼一個(gè)II型硫脂酶的基因的核苷酸序列，這個(gè)II型硫脂酶由序列16的氨基酸序列組成，命名為fscTE基因的核苷酸序列選自于序列1中包括17,556-18,413的一系列堿基。
本發(fā)明還提供了編碼2個(gè)糖基合成酶和1個(gè)糖苷轉(zhuǎn)移酶的基因的核苷酸序列，糖苷轉(zhuǎn)移酶由序列17的氨基酸序列組成，2個(gè)糖基合成酶(GDP-酮糖轉(zhuǎn)氨酶和GDP-甘露糖脫水酶)分別由序列18和序列19的氨基酸序列組成，命名為fscMI，fscMII，fscMIII基因的核苷酸序列選自于序列1中包括13,522-14,898，14,953-16,011，137,766-136,558的一系列堿基。
本發(fā)明還提供了編碼一個(gè)依賴FAD的單氧化酶的基因的核苷酸序列，這個(gè)依賴FAD的單氧化酶蛋白由序列20的氨基酸序列組成，命名為fscO基因的核苷酸序列選自于序列1中包括1,950-574的一系列堿基。
本發(fā)明還提供了編碼2個(gè)對(duì)氨基苯甲酸合成酶的基因的核苷酸序列，這兩個(gè)對(duì)氨基苯甲酸合成酶分別由序列21和22的氨基酸序列組成，命名為pabAB和pabC基因的核苷酸序列選自于序列1中包括18,610-20,781，3,037-2,264的一系列堿基。
本發(fā)明還提供了從至少攜帶有部分序列1重組載體中，或從微生物文庫(kù)中，或從微生物染色體DNA中分離FR-008生物合成基因的途徑。
本發(fā)明還提供了以至少來自于序列1聚酮合酶序列中的一個(gè)片段與來自于其它聚酮合酶基因簇的聚酮序列來構(gòu)建重組載體以獲得新型聚酮的途徑。
本發(fā)明還提供了在基因工程微生物體中提高FR-008或其衍生抗生素產(chǎn)量的途徑。
本發(fā)明還提供了簡(jiǎn)化FR-008的有效組分的途徑。
本發(fā)明還提供了得到至少包含部分序列1中DNA序列的重組DNA載體的途徑。
本發(fā)明還提供了產(chǎn)生被至少包含部分序列1中DNA序列的重組DNA載體轉(zhuǎn)化進(jìn)入的宿主細(xì)胞的途徑，此宿主細(xì)胞所產(chǎn)生的聚酮為FR-008或FR-008的結(jié)構(gòu)類似物。
本發(fā)明還提供了產(chǎn)生被至少包含部分序列1中DNA序列的重組DNA載體轉(zhuǎn)化進(jìn)入的宿主細(xì)胞的途徑，此宿主細(xì)胞所產(chǎn)生的FR-008或FR-008的結(jié)構(gòu)類似物產(chǎn)量得到提高或所產(chǎn)生的抗生素或某種代謝產(chǎn)物產(chǎn)量得到提高。
本發(fā)明還提供了產(chǎn)生在基因組中有FR-008生物合成基因被打斷，缺失或加倍的微生物體的途徑，至少其中之一的基因包含有序列1中的核苷酸序列。
本發(fā)明還提供了產(chǎn)生在基因組中有FR-008生物合成基因被改造的微生物體的途徑。
本發(fā)明還提供了產(chǎn)生在基因組中有FR-008生物合成酶中聚酮合酶結(jié)構(gòu)域之一或至少其中之一被缺失或加倍或改造的微生物體的途徑。
序列1的互補(bǔ)序列可依據(jù)DNA堿基互補(bǔ)原則隨時(shí)得到。序列1的核苷酸序列或部分核苷酸序列可以通過聚合酶鏈?zhǔn)椒磻?yīng)(PCR)或用合適的酶切相應(yīng)的DNA或使用其它合適的技術(shù)隨時(shí)得到。包含發(fā)明所提供序列或多個(gè)序列的DNA片段或基因可隨時(shí)得到。通過本發(fā)明所提供的核苷酸序列或部分核苷酸序列，可利用聚合酶鏈?zhǔn)椒磻?yīng)(PCR)的方法或包含本發(fā)明序列的DNA作為探針進(jìn)行Southern雜交的方法，從其它生物體得到與FR-008生物合成基因相似的基因。
本發(fā)明提供的核苷酸片段可用來從鏈霉菌FR-008或其它菌種中分離具生物活性的結(jié)構(gòu)域。例如，聚酮合酶結(jié)構(gòu)域和修飾基因的生物活性位點(diǎn)可以通過聚合酶鏈?zhǔn)椒磻?yīng)(PCR)，酶切位點(diǎn)或其它合適的技術(shù)得到。
本發(fā)明提供的核苷酸序列或多個(gè)序列可以與載體序列融合而得到重組序列和相應(yīng)的DNA分子。
包含本發(fā)明所提供核苷酸序列或至少部分序列的克隆基因或DNA片段可以通過打斷FR-008生物合成的一個(gè)或幾個(gè)合成步驟而得到新的FR-008衍生物。
包含本發(fā)明所提供核苷酸序列或至少部分序列的克隆基因或DNA片段可以通過打斷FR-008生物合成的一個(gè)或幾個(gè)修飾步驟而得到新的FR-008衍生物。
包含DNA片段或基因可以用來提高FR-008或其衍生物的產(chǎn)量。例如，轉(zhuǎn)入更多拷貝的正調(diào)節(jié)基因或打斷負(fù)調(diào)節(jié)基因。
包含本發(fā)明所提供核苷酸序列或至少部分序列的克隆DNA可用來從鏈霉菌FR-008基因組文庫(kù)中定位更多的文庫(kù)質(zhì)粒。這些文庫(kù)質(zhì)粒至少包含有本發(fā)明中的部分序列，也包含有鏈霉菌FR-008基因組中以前鄰近區(qū)域未克隆的DNA。
本發(fā)明所提供的核苷酸序列可以被修飾或突變。這些途徑包括插入或置換，聚合酶鏈?zhǔn)椒磻?yīng)，錯(cuò)誤介導(dǎo)聚合酶鏈?zhǔn)椒磻?yīng)，位點(diǎn)特異性突變，不同序列的重新連接，或通過紫外線或化學(xué)試劑。
本發(fā)明所提供的核苷酸序列可以通過序列的不同部分或其它來源的同源序列進(jìn)行直接進(jìn)化(DNA shuffling)。
通過缺失或失活來自于相同或不同聚酮合酶系統(tǒng)的一個(gè)或多個(gè)聚酮合酶結(jié)構(gòu)域，模塊或基因，或增加一個(gè)或多個(gè)聚酮合酶結(jié)構(gòu)域，模塊或基因而產(chǎn)生新的聚酮化合物。包含本發(fā)明的序列或至少部分序列的克隆基因可以通過合適的表達(dá)系統(tǒng)在外源宿主中表達(dá)以得到修飾的聚酮合酶或更高的生物活性或更高的產(chǎn)量．這些外源宿主包括鏈霉菌，大腸桿菌，芽孢桿菌，酵母，植物和動(dòng)物等。
包含本發(fā)明的核苷酸序列或至少部分序列的片段或結(jié)構(gòu)域或模塊或聚酮合酶基因可以用來構(gòu)建聚酮合酶庫(kù)或聚酮合酶衍生庫(kù)或組合庫(kù)。
包含本發(fā)明的核苷酸序列或至少部分序列的修飾基因，調(diào)節(jié)基因，轉(zhuǎn)運(yùn)基因或糖基合成和轉(zhuǎn)移酶基因可以用來構(gòu)建衍生庫(kù)或組合庫(kù)。
FR-008生物合成修飾基因，調(diào)節(jié)基因，轉(zhuǎn)運(yùn)基因或糖基合成和轉(zhuǎn)移酶基因的核苷酸序列提供了通過缺失或改造這些修飾基因，調(diào)節(jié)基因，轉(zhuǎn)運(yùn)基因或糖基合成和轉(zhuǎn)移酶基因而得到FR-008結(jié)構(gòu)衍生物或FR-008及其衍生物產(chǎn)量提高的途徑。
含有本發(fā)明的核苷酸序列或至少部分序列的一個(gè)片段或幾個(gè)片段可以克隆到改造后的細(xì)菌人工染色體(BAC)或酵母人工染色體(YAC)或柯斯載體(Cosmid)或表達(dá)載體或其它類型的載體，以應(yīng)合適的需要。
含有本發(fā)明的核苷酸序列或至少部分序列的基因或基因簇可以在異源宿主中表達(dá)并通過DNA芯片技術(shù)了解它們?cè)谒拗鞔x鏈中的角色。
包含本發(fā)明的氨基酸序列或至少部分序列(序列2-22)的多肽可能在去除或替代某個(gè)或某些氨基酸之后仍有生物活性甚至有新的生物學(xué)活性，或者提高了產(chǎn)量或優(yōu)化蛋白動(dòng)力學(xué)特征或其它致力于得到的性質(zhì)。
通過合適的技術(shù)缺失，連接本發(fā)明中的氨基酸序列可以得到新的蛋白或酶，進(jìn)而產(chǎn)生新的聚酮或相關(guān)連的產(chǎn)物。
本發(fā)明具有實(shí)質(zhì)性特點(diǎn)和顯著的進(jìn)步，本發(fā)明所提供的基因序列可用于產(chǎn)生預(yù)期的基因工程抗生素或雜合抗生素或用于提高抗生素或基因工程抗生素的產(chǎn)量。本發(fā)明所提供的氨基酸序列可以用來分離需要的蛋白質(zhì)并可以用于抗體制備。本發(fā)明所提供的氨基酸序列提供了預(yù)測(cè)聚酮合酶三維結(jié)構(gòu)的可能，進(jìn)而為改造或改善蛋白活性提供依據(jù)。本發(fā)明所提供的基因及其所編碼的蛋白質(zhì)蛋白質(zhì)，相應(yīng)的抗體或多核苷可以用來查找和發(fā)展可用于醫(yī)藥，工業(yè)，農(nóng)業(yè)的化合物或蛋白。


以下通過附圖對(duì)本發(fā)明進(jìn)一步描述圖1為用來測(cè)序覆蓋整個(gè)FR-008基因簇的文庫(kù)柯斯質(zhì)粒和亞克隆圖2為負(fù)責(zé)FR-008生物合成的整個(gè)FR-008基因簇的結(jié)構(gòu)。包括結(jié)構(gòu)基因，修飾基因，調(diào)節(jié)基因，矯正基因及轉(zhuǎn)運(yùn)基因等，負(fù)責(zé)FR-008的生物合成及調(diào)節(jié)和外運(yùn)。
圖3為FR-008化學(xué)結(jié)構(gòu)示意圖。編號(hào)為C1’-C6’的為氨基海藻糖基團(tuán)。羧基化發(fā)生在C-18位，羥基化發(fā)生在C-9位。R1代表羥基或酮基，R2代表羥基或H原子。
圖4為調(diào)節(jié)基因突變株基因置換發(fā)生的雜交證明。A為基因置換發(fā)生的示意圖；B為基因突變株的雜交證明。HJ-1，HJ-2，HJ-3與1.5kb雜交的陽性帶(4.3kb)比野生型的陽性帶(1.5kb)大2.8kb，因?yàn)镠J-1，HJ-2，HJ-3的染色體中阿泊拉霉素/紅霉素抗性基因置換了調(diào)節(jié)基因。
圖5為調(diào)節(jié)基因突變株生物活性測(cè)定。左右下方3個(gè)突變株均不再產(chǎn)生抑菌圈。正上方為野生型對(duì)照。
圖6為負(fù)責(zé)FR-008分子中雙鍵和糖基附著的酮基還原酶結(jié)構(gòu)域(KR)控制立體構(gòu)型特定氨基酸附近的氨基酸的比較。KR8，KR9和KR12的這個(gè)特定氨基酸由天冬氨酸分別變?yōu)楦拾彼?G)，天冬酰氨(N)和丙氨酸(A)。
具體實(shí)施例方式
如圖1和圖2所示，本發(fā)明序列列表中序列描述序列1為包括21個(gè)開放讀碼框架的138,203bp的核苷酸序列，它們是負(fù)責(zé)FR-008生物合成的基因fscA，fscB，fscC，fscD，fscE，fscF，fscTI，fscTII，fscRI，fscRII，fscRIII，fscRIV，fscP，fscFE，fscTE，fscMI，fscMII，fscMIII，fscO，pabAB和pabC。
序列2為fscA基因(序列1中核苷酸20,927-26,158)編碼的I型聚酮合酶(FscA)的氨基酸序列。
序列3為fscC基因(序列1中核苷酸60,860-28,983)編碼的I型聚酮合酶(FscC)的氨基酸序列。
序列4為fscB基因(序列1中核苷酸77,587-60,962)編碼的I型聚酮合酶(FscB)的氨基酸序列。
序列5為fscF基因(序列1中核苷酸84,132-77,983)編碼的I型聚酮合酶(FscF)的氨基酸序列。
序列6為fscE基因(序列1中核苷酸107,485-84,170)編碼的I型聚酮合酶(FscE)的氨基酸序列。
序列7為fscD基因(序列1中核苷酸136,148-107,496)編碼的I型聚酮合酶(FscD)的氨基酸序列。
序列8為fscTI基因(序列1中核苷酸26,333-27,340)編碼的ABC轉(zhuǎn)運(yùn)蛋白(FscTI)的氨基酸序列。
序列9為fscTII基因(序列1中核苷酸27,561-28,280)編碼的ABC轉(zhuǎn)運(yùn)蛋白(FscTII)的氨基酸序列。
序列10為fscRI基因(序列1中核苷酸3,818-3,150)編碼的調(diào)節(jié)蛋白(FscRI)的氨基酸序列。
序列11為fscRII基因(序列1中核苷酸7,205-4,377)編碼的調(diào)節(jié)蛋白(FscRII)的氨基酸序列。
序列12為fscRIII基因(序列1中核苷酸10,320-7,210)編碼的調(diào)節(jié)蛋白(FscRIII)的氨基酸序列。
序列13為fscRIV基因(序列1中核苷酸13,315-10,298)編碼的調(diào)節(jié)蛋白(FscRIV)的氨基酸序列。
序列14為fscP基因(序列1中核苷酸16,106-17,287)編碼的細(xì)胞色素P450單氧化酶(FscP)的氨基酸序列。
序列15為fscFE基因(序列1中核苷酸17,334-17,528)編碼的鐵氧化還原蛋白(FscFE)的氨基酸序列。
序列16為fscTE基因(序列1中核苷酸17,556-18,413)編碼的II型硫脂酶(FscTE)的氨基酸序列。
序列17為fscMI基因(序列1中核苷酸13,522-14,898)編碼的糖苷轉(zhuǎn)移酶(FscMI)的氨基酸序列。
序列18為fscMII基因(序列1中核苷酸14,953-16,011)編碼的GDP-酮糖氨基轉(zhuǎn)移酶(糖基合成酶)(FscMII)的氨基酸序列。
序列19為fscMIII基因(序列1中核苷酸137,766-136,558)編碼的GDP-甘露糖-4，6-脫水酶(糖基合成酶)(FscMIII)的氨基酸序列。
序列20為fscO基因(序列1中核苷酸1,950-574)編碼的依賴FAD的單氧化酶(FscO)的氨基酸序列。
序列21為pabAB基因(序列1中核苷酸18,610-20,781)編碼的4-氨基4-脫氧chorismate合成酶(對(duì)氨基苯甲酸合成酶)(PabAB)的氨基酸序列。
序列22為pabC基因(序列1中核苷酸3,037-2,264)編碼的4-氨基-4-脫氧chorismate合成酶(對(duì)氨基苯甲酸合成酶)(PabC)的氨基酸序列。
以下進(jìn)一步對(duì)本發(fā)明詳細(xì)描述由于FR-008有抗真菌活性，對(duì)蚊子幼蟲有高毒性和可以防止前列腺腫大以及可以防止陰道滴蟲感染和念珠菌陰道炎以及在其它工業(yè)，農(nóng)業(yè)，醫(yī)藥上所具有的應(yīng)用潛能，本發(fā)明分離了負(fù)責(zé)FR-008生物合成的基因簇，覆蓋整個(gè)基因簇的五個(gè)柯斯質(zhì)粒，pHZ145，pHZ137，pHZ220，pHZ194和pJTU1連同一個(gè)亞克隆質(zhì)粒pJTU6被用來測(cè)序，pJTU6攜帶了pHZ141的5.2kb和7.5kb BamHI片段，但是7.5kb對(duì)5.2kb片段的方向與pHZ141或者說染色體中是相反的，pHZ145，pHZ137，pHZ194和pJTU10的載體為pHZ132，pHZ220的載體為pIJ653，pJTU6的載體為pBluescriptII SK(+)。
測(cè)序獲得138,203bp的連續(xù)核苷酸序列。在全部21個(gè)開放讀碼框架中有6個(gè)I型聚酮合酶基因(fscA-fscF)。序列分析是使用FramePlot 2.3.2軟件和美國(guó)國(guó)家生物信息中心的Conserved Domain Database search及其提供的世界范圍的Blast引擎。fscA-fscF編碼的氨基酸序列通過與已經(jīng)得到充分了解的一些I型聚酮合酶的氨基酸序列進(jìn)行比較從而得出這些多肽的功能特征(詳見圖1)。
聚酮合酶基因序列序列1中的20,927-26,158和28,983-136,148堿基是負(fù)責(zé)FR-008內(nèi)酯環(huán)合成的聚酮合成酶基因的序列，F(xiàn)R-008基因簇中共有6個(gè)聚酮合酶基因，在fscA和fscC之間有一段編碼兩個(gè)ABC轉(zhuǎn)運(yùn)子的核苷酸序列。FR-008基因簇中各聚酮合酶基因的核苷酸序列與相應(yīng)的氨基酸序列，如表1所示表1

fscA編碼FR-008內(nèi)酯環(huán)合成的起始模塊和延伸模塊1(序列1中堿基20,927-26,158)。
起始模塊和延伸模塊1中的結(jié)構(gòu)域在FscA的位置如表2所示表2FscA

fscC編碼FR-008內(nèi)酯環(huán)合成的六個(gè)延伸模塊(延伸模塊10，9，8，7，6，5序列1中堿基60,860-28,983)這些延伸模塊中的結(jié)構(gòu)域在FscC的位置如表3所示表3FscC


fscB編碼FR-008內(nèi)酯環(huán)合成的3個(gè)延伸模塊(延伸模塊4，3，2，序列1中堿基77,587..60,962)這些延伸模塊中的結(jié)構(gòu)域在FscB的位置如表4所示表4FscB


fscF編碼FR-008內(nèi)酯環(huán)合成的延伸模塊21和一個(gè)硫脂酶結(jié)構(gòu)域。
延伸模塊中的結(jié)構(gòu)域在FscF的位置如表5所示表5FscF

fscE編碼FR-008內(nèi)酯環(huán)合成的4個(gè)延伸模塊(延伸模塊20，19，18，17，序列1中堿基107,485-84,170)。
這些延伸模塊中的結(jié)構(gòu)域在FscE的位置如表6所示表6FscE


fscD編碼FR-008內(nèi)酯環(huán)合成的6個(gè)延伸模塊(延伸模塊16，15，14，13，12，11，序列1中堿基136,148-107,496)。
這些延伸模塊中的結(jié)構(gòu)域在FscD的位置如表7所示表7FscD


FR-008聚酮鏈的修飾基因除6個(gè)聚酮合酶基因外，在FR-008基因簇中還有15個(gè)編碼修飾酶，調(diào)節(jié)蛋白和轉(zhuǎn)運(yùn)蛋白的基因。
表8顯示了它們的核苷酸和氨基酸序列表8


鏈霉菌FR-008中各基因在FR-008中合成中的功能如表9和表10所示表9

表10


(ADC為4-氨基-4-脫氧chorismate的縮寫)FR-008生物合成的聚酮合酶基因fscA與fscC，fscB，fscF，fscE和fscD的轉(zhuǎn)錄方向相反，與FR-008碳鏈合成21個(gè)縮合步驟一致，在FR-008基因簇的fscA-fscF基因中有21個(gè)延伸模塊分布在它們所編碼的聚酮合酶中。
FscA蛋白包含一個(gè)FR-008生物合成起始的起始模塊和延伸模塊1，延伸模塊1包含KS-AT-ACP結(jié)構(gòu)域，在納巴霉素，匹馬菌素和殺念菌素生物合成基因簇中也發(fā)現(xiàn)了類似CoL結(jié)構(gòu)域的存在。
FscB包含了FR-008碳鏈合成延伸步驟2-4所需的延伸模塊2-4，F(xiàn)scB中的3個(gè)AT結(jié)構(gòu)域在活性位點(diǎn)GHSXG的N末端都有一個(gè)RVDVV×××××××M×S(A)×A××W的保守序列基元，具有這個(gè)保守序列基元的AT結(jié)構(gòu)域是甲基丙二酰特異性的(mAT)，即它只識(shí)別底物甲基丙二酰，模塊2包含KS，mAT，KR和ACP結(jié)構(gòu)域，模塊3有一個(gè)DH-ER-KR的還原環(huán)，模塊4中的DH4結(jié)構(gòu)域缺失了N末端的保守序列HPLL并在C末端缺失了56氨基酸，所以是無活性的。
FscC和FscD都是包含6個(gè)延伸模塊的巨大蛋白。FscC長(zhǎng)10,625氨基酸，F(xiàn)scD長(zhǎng)9,550氨基酸。其它基因簇中包含6模塊的Raps2，NysC，NysI，AmphC，AmphI和PIMS2都是穩(wěn)定的，但迄今未發(fā)現(xiàn)包含7個(gè)模塊的聚酮合酶，7模塊蛋白的保真性和連續(xù)性被認(rèn)為不足以得到準(zhǔn)確的翻譯。
FscC負(fù)責(zé)合成6個(gè)連續(xù)的雙鍵(FR-008分子結(jié)構(gòu)中共有7個(gè)連續(xù)的雙鍵)，負(fù)責(zé)延伸步驟5-10的模塊5-10都包含相同的DH-KR還原環(huán)，其中6個(gè)DH結(jié)構(gòu)域都有保守的活性位點(diǎn)基元G×G××G×××A，F(xiàn)scC中所有的AT結(jié)構(gòu)域都是丙二酰特異性的。FscC組裝FR-008大部分的多烯結(jié)構(gòu)。
FscD由延伸模塊11-16組成，負(fù)責(zé)FR-008碳鏈合成的縮合步驟11-16，模塊11的結(jié)構(gòu)域結(jié)構(gòu)為(KS-AT-DH-KR-ACP)，合成7個(gè)雙鍵中的最后1個(gè)雙鍵。模塊13有一個(gè)甲基丙二酰特異性的AT結(jié)構(gòu)域，它的結(jié)構(gòu)是KS-mAT-KR-ACP，會(huì)在延伸步驟13整合一個(gè)甲基丙二酰輔酶A延伸單位，模塊12，14，15和16都是同樣的KS-AT-KR-ACP結(jié)構(gòu)域結(jié)構(gòu)，但KR15是沒有活性的。因?yàn)樵贙R15的NADP結(jié)合基元缺少兩個(gè)保守的G殘基并在中部有10氨基酸的缺失。這樣一個(gè)無活性的KR會(huì)使C-15上成為一個(gè)羰基，進(jìn)而可以與C-19位的羥基形成FR-008分子中的六元半縮醛環(huán)。
FscE蛋白由負(fù)責(zé)延伸步驟17-20的模塊17-20組成，模塊18和20都包含一個(gè)完整的DH-ER-KR還原環(huán)，將導(dǎo)致延伸步驟18和20被完全還原為脂肪鏈。模塊17中的DH與ER結(jié)構(gòu)域是無活性的。模塊19的結(jié)構(gòu)域?yàn)镵S-AT-KR-ACP。KR19的活性位點(diǎn)中的酪氨酸被亮氨酸取代。
FscF包含了模塊21和一個(gè)位于C末端鏈延伸終止的硫脂酶(TE)結(jié)構(gòu)域。FscF中的DH結(jié)構(gòu)域在最后的延伸步驟中并不需要，這種完整但沒有功能的DH結(jié)構(gòu)域在制霉菌素(NysK)，利福霉素(RifB和RifC)和兩性霉素(AmphJ)的聚酮合酶中也有發(fā)現(xiàn)。FscF的KS-AT-KR-ACP結(jié)構(gòu)域結(jié)構(gòu)將會(huì)在FR-008內(nèi)酯環(huán)的C-3位產(chǎn)生一個(gè)羥基(-OH)。而且，也發(fā)現(xiàn)有FR-008 C-3位是酮基的結(jié)構(gòu)(此時(shí)KR21沒有功能)存在。說明KR21是部分行使功能的結(jié)構(gòu)域。FscF中的硫脂酶結(jié)構(gòu)域使成熟的FR-008聚酮鏈從聚酮合酶上掉下來并形成內(nèi)酯環(huán)。
除了FscF中的硫脂酶結(jié)構(gòu)域，在pabAB基因的上游還有一個(gè)編碼II型硫脂酶的fscTE基因。fscTE顯示了與II型硫脂酶基因的高度同源性，可去除ACP巰基上無反應(yīng)活性的乙酸根，丙酸根或丁酸根。
FR-008起始單位PABA生物合成的基因PabAB和PabC基因的產(chǎn)物負(fù)責(zé)合成對(duì)氨基苯甲酸(PABA)，對(duì)氨基苯甲酸是FR-008聚酮合成起始單位芳香基團(tuán)的前體，PabAB與其它來源的pabAB具有很高的同源性。PabC在鏈霉菌中是首次發(fā)現(xiàn)。PabAB是4-氨基-4-脫氧chorismate合成酶，將chorismate和谷氨酸鹽轉(zhuǎn)化為4-氨基-4-脫氧chorismate和谷氨酸酯。PabC是4-氨基-4-脫氧chorismate裂解酶，將4-氨基-4-脫氧chorismate轉(zhuǎn)化為芳香化的對(duì)氨基苯甲酸和丙酮酸鹽。PabAB與來自灰色鏈霉菌IMRU3570的PabAB顯示93％的同源性，與來自S.venezuelae的PabAB顯示46％的同源性。PabC與Mycobacteriumtuberculosis H37Rv和Pseudomonas aeruginosa PA01的PabC均顯示32％的同源性。
調(diào)節(jié)區(qū)域的基因FR-008基因簇中有4個(gè)調(diào)節(jié)基因fscRI，fscRII，fscRIII和fscRIV，它們的產(chǎn)物均屬于LuxR轉(zhuǎn)錄調(diào)節(jié)子家族。
fscRI編碼的調(diào)節(jié)蛋白FscRI是一個(gè)222氨基酸的蛋白質(zhì)，與除蟲鏈霉菌多烯大環(huán)內(nèi)酯生物合成基因簇中的PleR有66％的同一性(基因銀行序號(hào)AB070949)，與制霉菌素生物合成中的起調(diào)節(jié)作用的ORF4有59％的同一性。FscRI還與其它雙組分的調(diào)節(jié)子顯示同源性。
fscRII編碼的調(diào)節(jié)蛋白FscRII(942氨基酸)與灰色鏈霉菌中可能對(duì)殺念菌素合成起調(diào)節(jié)作用的595氨基酸的蛋白有很高的同源性(在前595氨基酸84％同一性，但FscRII比它長(zhǎng)347氨基酸)。FscRII與制霉菌素中NysRIII(927氨基酸)有42％同一性。
fscRIII編碼的調(diào)節(jié)蛋白FscRIII是1,036氨基酸的蛋白質(zhì)，與NysRII(953氨基酸)具有34％的同源性。fscRIV編碼的調(diào)節(jié)蛋白FscRIV(1,005氨基酸)與NysRI(966氨基酸)具有46％的同源性，NysRII和NysRI都是制霉菌素基因簇中起正調(diào)節(jié)作用的基因。
與NysRIII，NysRII和NysRI相似的是，F(xiàn)scRII，F(xiàn)scRIII和FscRIV與鏈霉菌亞種SA-COO中和膽固醇氧化酶細(xì)胞色素P450操縱子相鄰的轉(zhuǎn)錄調(diào)節(jié)子具有很高的同源性。
多烯抗生素FR-008基因簇包含非常巨大的聚酮合酶基因(fscC32kb；fscD29kb；fscE23kb)，而且其中一些聚酮合酶基因從它們之間組織結(jié)構(gòu)來說是共轉(zhuǎn)錄的。這種轉(zhuǎn)錄因而會(huì)非常長(zhǎng)。轉(zhuǎn)錄調(diào)節(jié)子可能參與維持這些長(zhǎng)mRNA的穩(wěn)定。
氨基海藻糖生物合成和附著基因NMR數(shù)據(jù)顯示FR-008分子的糖基是氨基海藻糖。在FR-008基因簇中有3個(gè)基因(fscMI，fscMII和fscMIII)負(fù)責(zé)氨基海藻糖的生物合成與附著。
FscMI(458氨基酸)與真核生物的UDP-葡萄糖苷酰轉(zhuǎn)移酶具有很高的同源性，負(fù)責(zé)氨基海藻糖在FR-008配基上C-21位的附著。FscMII(352氨基酸)與4，6-二脫氧-4-氨基甘露糖合成中的氨基轉(zhuǎn)移酶相似。fscMIII基因編碼的FscMIII(402氨基酸)與GDP-甘露糖-4，6-脫水酶相似。氨基海藻糖(3，6-二脫氧-3-氨基-D-甘露糖)的生物合成途徑包括來自主代謝中的GDP-甘露糖在GDP-甘露糖脫水酶的作用下轉(zhuǎn)化為GDP-4-酮基-6-脫氧-D-甘露糖，然后異構(gòu)化為GDP-3-酮基-6-脫氧-D-甘露糖，最后通過GDP-酮糖轉(zhuǎn)氨酶的轉(zhuǎn)氨作用形成GDP-氨基海藻糖。其中異構(gòu)化并不需要酶的參與。FscMIII和FscMII分別負(fù)責(zé)此生物合成途徑的脫水和轉(zhuǎn)氨作用。
當(dāng)在基因銀行搜索時(shí)，F(xiàn)scMI與AmphDI有54.7％的同一性，與NysDI有52.4％的同一性，與PimK有60.5％同一性。FscMII與CanA有92％的同一性，與AmphDII，NysDII和PimC分別有74-76％的同一性。FscMIII與AmphDIII(66％)，NysDIII(67％)和PimJ(69％)有很高的同一性。以上提到的酶均被認(rèn)為負(fù)責(zé)兩性霉素(AmphDI，AmphDII和AmphDIII)，制霉菌素(NysDI，NysDII和NysDIII)，匹馬菌素(PimK，PimC和PimJ)或殺念菌素(CanA)分子中氨基海藻糖的生物合成。這也符合FscMI，F(xiàn)scMII和FscMIII在FR-008基因簇中負(fù)責(zé)氨基海藻糖生物合成與附著的功能。
修飾與轉(zhuǎn)運(yùn)基因負(fù)責(zé)FR-008聚酮鏈合成的聚酮合酶FscD中的甲基丙二酰特異性的AT13(?；D(zhuǎn)移酶結(jié)構(gòu)域)在第13個(gè)延伸步驟中導(dǎo)入一個(gè)丙酸殘基，因而在FR-008分子的C-18位形成一個(gè)甲基側(cè)鏈。細(xì)胞色素P450單氧化酶FscP(393氨基酸)，負(fù)責(zé)將C-18位的甲基側(cè)鏈氧化為羧基基團(tuán)。緊鄰fscP下游的fscFE基因編碼一個(gè)小的酸性蛋白(64氨基酸)，是含有鐵硫中心簇([3Fe-4S])的鐵氧化還原蛋白。它作為一個(gè)電子傳遞蛋白成為P450單氧化酶系統(tǒng)的一部分。FscP與負(fù)責(zé)匹馬霉素后修飾的細(xì)胞色素P450單氧化酶PimG顯示81％的同源性和69％的同一性，與負(fù)責(zé)制霉菌素后修飾的細(xì)胞色素P450單氧化酶NysN顯示77％的同源性和66％的同一性，與負(fù)責(zé)兩性霉素后修飾的細(xì)胞色素P450單氧化酶AmphN顯示76％的同源性和66％的同一性，與負(fù)責(zé)殺念菌素后修飾的細(xì)胞色素P450單氧化酶顯示94％的同源性。
負(fù)責(zé)FR-008聚酮鏈合成的聚酮合酶FscE中的模塊18是一個(gè)包括完整還原環(huán)的KS-AT-DH-ER-KR-ACP結(jié)構(gòu)，因而在第18個(gè)延伸步驟中被完全還原成脂肪鏈而在C-9位無基團(tuán)。fscO所編碼依賴FAD的單氧化酶(FscO)負(fù)責(zé)在C-9位修飾而引入羥基。這與兩性霉素和制霉菌素后修飾羥基化中亦由細(xì)胞色素P450單氧化酶負(fù)責(zé)不同。FscO與Agrobacterium tumefaciens C58中的依賴FAD的單氧化酶顯示34％的同源性和25％的同一性。與KR21一樣，fscO也是部分活性的，因此在FR-008復(fù)合物中有C-9位羥基化與不羥基化的兩種結(jié)構(gòu)共同存在。
FR-008基因簇與殺念菌素基因簇的序列比較
FR-008含有一個(gè)與灰色鏈霉菌中的殺念菌素D相同的含4-氨基苯乙酮的七烯大環(huán)內(nèi)酯配基，鏈霉菌FR-008中存在130kb的pHZ227和30kb的pHZ228兩個(gè)線性質(zhì)粒，但殺念菌素產(chǎn)生菌灰色鏈霉菌IMRU3570沒有線性質(zhì)粒，鏈霉菌FR-008產(chǎn)孢非常好而灰色鏈霉菌IMRU3570產(chǎn)孢很差，因?yàn)镕R-008和殺念菌素D具有相似的結(jié)構(gòu)，所以負(fù)責(zé)合成它們的序列很可能會(huì)有很高的同源性，殺念菌素生物合成基因簇只有部分序列獲得認(rèn)定(共39,314bp的序列)，F(xiàn)R-008基因簇(序列1，共138,203bp)中相對(duì)應(yīng)的序列和這39,314bp序列對(duì)比發(fā)現(xiàn)它們之間有很高的同源性，F(xiàn)R-008基因簇(序列1)中的堿基5,421到44,805(從fscRII到fscB模塊4中的KS結(jié)構(gòu)域)與這39,314bp序列除一些小的間隔序列外有98-99％同源性，這些間隔序列在兩個(gè)序列中沒有明顯的同源性。
表11顯示了這兩段序列的對(duì)比表11

表12顯示了間隔序列及它們?cè)趦蓚€(gè)基因簇中的位置。
表12

以上表明FR-008和殺念菌素基因簇來源于很相近的祖先。
以下的應(yīng)用實(shí)例闡明了得到和應(yīng)用本發(fā)明所提供序列和要素的優(yōu)選的途徑。這些實(shí)例只是用做說明而并不限制本發(fā)明的應(yīng)用范圍。通過基因工程分子操作基因簇中的一些基因序列，可以得到序列改變的衍生的基因工程菌株，或利用本發(fā)明序列得到攜帶FR-008生物合成基因的DNA片段。
應(yīng)用實(shí)例1缺失序列1中80kb序列1,518bp BglII-BamHI片段(序列1中堿基2,293-3,810)和4,075bp BamHI-BglII片段(序列1中堿基83,766-87,840)被選擇用來作為基因置換的兩臂來缺失基因簇中79,955bp的序列(序列1中3,811-83,765)。這79,955bp序列是從fscRI序列到fscF中模塊11的KS21結(jié)構(gòu)域所對(duì)應(yīng)的序列。1,518bp片段是從pHZ145中回收，而4,075bp片段是從pHZ144中回收。它們均被用來與FR-008染色體DNA進(jìn)行同源重組。在這兩個(gè)片段中間插入一個(gè)阿泊拉霉素抗性基因，然后把得到的結(jié)構(gòu)克隆入pIJ903而得到基因置換質(zhì)粒pHZ2029。通過接合轉(zhuǎn)移將pHZ2029導(dǎo)入鏈霉菌FR-008發(fā)生雙交換后而得到基因置換菌株。通過Southern雜交驗(yàn)證了雙交換的發(fā)生。另外通過高壓脈沖電泳發(fā)現(xiàn)基因置換菌株中染色體約300kb VspI片段消失而產(chǎn)生了一個(gè)220kb的新片段，顯示在此基因置換菌株中FR-008基因簇從fscRI到fscF模塊11的KS21結(jié)構(gòu)域的約80kb序列被去除。此基因置換菌株中FR-008的生物合成被阻斷了。通過檢測(cè)發(fā)現(xiàn)對(duì)啤酒酵母的失去了抑制活性。
此基因置換顯示分離到的序列(序列1)確實(shí)是負(fù)責(zé)FR-008抗生素生物合成的。
應(yīng)用實(shí)例2缺失聚酮合酶基因序列下游包含聚酮合酶基因序列的fscA(20,927-26,158)，fscC(60,860-28,983)，fscB(77,587-60,962)，fscF(84,132-77,983)，fscE(107,485-84,170)，fscD(137,148-107,496)負(fù)責(zé)FR-008配基的生物合成。在fscA和fscC之間有兩個(gè)ABC轉(zhuǎn)運(yùn)蛋白(fscTI，fscTII)，它們包括序列1中的堿基26,333到28,280。文庫(kù)質(zhì)粒pHZ119攜帶了pabAB基因序列及其下游包括這兩個(gè)ABC轉(zhuǎn)運(yùn)基因在內(nèi)的聚酮合酶序列。pHZ119 BglII酶切后將其上的兩個(gè)BglII片段置換成大腸桿菌質(zhì)粒pDH5。這兩個(gè)BglII片段編碼了從fscA中的ATP依賴性Carboxylic acid輔酶連接酶結(jié)構(gòu)域(CoL)到fseC模塊9的ACP9結(jié)構(gòu)域(包括fscTI和fscTII)。所得到的質(zhì)粒被用來進(jìn)行基因置換。發(fā)生雙交換之后，野生型FR-008染色體上序列1中堿基21,537到36,847被pDH5序列所取代。雙交換被Southern雜交所證實(shí)。生物活性測(cè)定實(shí)驗(yàn)發(fā)現(xiàn)雙交換菌株的FR-008生物合成被阻斷。這表明在鏈霉菌FR-008染色體上從fscA中ATP依賴性Carboxylic acid輔酶連接酶結(jié)構(gòu)域(CoL)到fscC模塊9的ACP9結(jié)構(gòu)域所對(duì)應(yīng)的序列被pDH5序列取代后，F(xiàn)R-008抗生素的生物合成被阻斷。這些序列被證明對(duì)FR-008生物合成是必需的。
應(yīng)用實(shí)例3將pHZ168載體序列插入到序列1中的聚酮合酶序列中有一個(gè)1,424bp BamHI片段(序列1中的堿基50,488到51,911)編碼fscC模塊6中的ACP6結(jié)構(gòu)域。通過BamHI酶切回收pHZ137上包含此1,424bp序列的DNA片段，克隆到pHZ168上。pHZ168是pDH5的衍生質(zhì)粒，包含RK2中的oriT(接合轉(zhuǎn)移起始位點(diǎn))。所得到的基因中斷結(jié)構(gòu)通過接合轉(zhuǎn)移導(dǎo)入鏈霉菌FR-008中。因pHZ168沒有鏈霉菌復(fù)制子，它及它攜帶1,424bp片段的衍生結(jié)構(gòu)在鏈霉菌FR-008中不能復(fù)制。這個(gè)基因中斷結(jié)構(gòu)只有通過其與染色體相同的1,424bp序列同源重組到鏈霉菌FR-008染色體上才能復(fù)制?；蛑袛嗑瓯怀晒Φ姆蛛x得到。這些基因中斷突變菌株在負(fù)責(zé)FR-008生物合成的聚酮合酶DNA序列中插入了pHZ168。更確切地說，pHZ168載體序列被插入到fscC模塊6的ACP6結(jié)構(gòu)域序列中。生物活性測(cè)定表明這些突變菌株的FR-008生物合成因pHZ168的插入而被阻斷。
應(yīng)用實(shí)例2和3都表明本發(fā)明所提供的聚酮合酶基因負(fù)責(zé)FR-008內(nèi)酯環(huán)的合成。由于每一個(gè)聚酮合酶模塊對(duì)應(yīng)一個(gè)縮合步驟，從而對(duì)應(yīng)FR-008的內(nèi)酯環(huán)分子結(jié)構(gòu)，這提供了一個(gè)改造鏈霉菌FR-008中聚酮合酶基因序列而得到FR-008抗生素相應(yīng)內(nèi)酯環(huán)結(jié)構(gòu)改變的新的抗生素的途徑。這種新的抗生素可能具有抗菌活性提高或抗菌譜改變或抗生素化學(xué)特性改變或化學(xué)毒性改變的特性，從而提高抗生素工業(yè)生產(chǎn)的經(jīng)濟(jì)效益或擴(kuò)大臨床應(yīng)用的范圍。
應(yīng)用實(shí)例4缺失修飾基因序列pabAB和pabC基因可能參與FR-008組裝中起始單位對(duì)氨基苯甲酸的生物合成。在pabAB上游，有9個(gè)修飾基因和調(diào)節(jié)基因。與應(yīng)用實(shí)例2中去除FR-008染色體上pabAB下游15,311bp聚酮合酶序列方法類似，將pHZ138上包含序列1中堿基2,293到21,536的19,244bp的BglII片段置換成pDH5。通過基因置換實(shí)驗(yàn)，鏈霉菌FR-008染色體上包括部分pabC一直到pabAB基因的19,244bp序列被pDH5載體序列所取代。生物活性測(cè)定表明基因置換菌株中FR-008七烯大環(huán)內(nèi)酯的生物合成被阻斷。
而且，1,518bp BglII-BamHI片段和4,290bp BamHI-BglIII片段亦被選來構(gòu)建基因置換質(zhì)粒以去除它們中間13,436bp的序列。這13,436bp的序列編碼部分fscRI基因到pabAB基因。在此兩個(gè)片段之間插入阿泊拉霉素抗性基因并克隆到自殺載體pHZ660上。雙交換后鏈霉菌FR-008染色體上此13,436bp序列被阿泊拉霉素抗性基因替代。通過Southern雜交確證了雙交換的發(fā)生，所得到的基因置換衍生菌株不能產(chǎn)生FR-008抗生素，顯示這些修飾基因和調(diào)節(jié)基因是FR-008生物合成所必需的。
這提供了一個(gè)改造鏈霉菌FR-008中修飾酶基因序列而得到FR-008抗生素相應(yīng)結(jié)構(gòu)改變的新的抗生素的途徑。修飾酶通過作用于FR-008的內(nèi)酯環(huán)而使內(nèi)酯環(huán)的相應(yīng)結(jié)構(gòu)被修飾。FscP氧化縮合步驟13所形成的甲基成為羧基，而FscO在C-9位加羥基。例如中斷或缺失FscP基因而得到C-18位為甲基而非羧基的FR-008抗生素衍生物；或中斷或缺失FscO而得到C-9位無羥基的FR-008抗生素衍生物；或同時(shí)中斷或缺失兩個(gè)甚至三個(gè)修飾基因而得到相應(yīng)的FR-008抗生素衍生物。還可以轉(zhuǎn)入外源的修飾酶基因而得到相應(yīng)結(jié)構(gòu)改變的FR-008抗生素衍生物。所有這些新的FR-008抗生素衍生物可能具有抗菌活性提高或抗菌譜改變或抗生素化學(xué)特性改變或化學(xué)毒性改變的特性，從而提高抗生素工業(yè)生產(chǎn)的經(jīng)濟(jì)效益或擴(kuò)大臨床應(yīng)用的范圍。
相似的，以5,069bp BamHI片段(序列1中堿基5,090到10,159)和4,290bpBamHI-BglII片段(序列1中堿基17,247到21,537)作為同源重組的片段，序列1中從堿基5,091到17,246編碼fscRIV，fscMI，fscMII，fscP和部分fscRIII序列被阿泊拉霉素抗性基因取代。生物活性測(cè)定表明這些基因置換菌株衍生菌株也不能產(chǎn)生FR-008抗生素。此7,088bp序列是FR-008生物合成必需的。
應(yīng)用實(shí)例5缺失調(diào)節(jié)基因序列利用鏈霉菌FR-008文庫(kù)柯斯質(zhì)粒pHZ138上1,518bp BglII-BamHI片段和7,088bp BamHI片段作為同源整合片段，以與天然染色體相同的方向克隆到pHZ1358(硫鏈絲菌素抗性)上，中間插入了阿泊拉霉素/紅霉素抗性基因，得到pHZ2007。將pHZ2007通過接合轉(zhuǎn)移導(dǎo)入鏈霉菌FR-008中，通過反選擇篩選阿泊拉霉素/紅霉素抗性而硫鏈絲菌素敏感的接合轉(zhuǎn)移子。以1.5kb和1.9kb以及阿泊拉霉素/紅霉素抗性基因片段為探針的雜交實(shí)驗(yàn)表明(圖4)，所篩選到的阿泊拉霉素/紅霉素抗性而硫鏈絲菌素敏感的接合轉(zhuǎn)移子(基因置換菌株菌株)均缺失了編碼fscRII，fscRIII和部分fscRI的6,414bp序列(序列1中堿基3,811到10,158)而被阿泊拉霉素/紅霉素抗性基因所替代。這個(gè)區(qū)域代表調(diào)節(jié)基因的序列。生物測(cè)定表明調(diào)節(jié)基因的序列缺失的突變株中FR-008的生物合成被阻斷(圖5)。顯示這些調(diào)節(jié)基因?qū)R-008生物合成是必需的。
應(yīng)用實(shí)例6缺失起始單位對(duì)氨基苯甲酸合成基因序列從pHZ145中回收攜帶對(duì)氨基苯甲酸合成基因pabAB的4.6kb的BamHI片段，克隆到自殺載體pOJ260上，通過FspI酶切自連缺失外源片段中FspI酶切位點(diǎn)之間1,491bp的片段，即同框缺失了pabAB基因內(nèi)部的497氨基酸的序列。將得到的基因置換質(zhì)粒通過接合轉(zhuǎn)移導(dǎo)入鏈霉菌FR-008中，篩選到發(fā)生了預(yù)期的pabAB基因缺失1,491bp的突變菌株并通過雜交驗(yàn)證。突變株中未能檢測(cè)到FR-008的合成。說明缺乏對(duì)氨基苯甲酸，F(xiàn)R-008的生物合成不能起始。在培養(yǎng)基中添加0.3M的外來對(duì)氨基苯甲酸，突變株又恢復(fù)了FR-008的產(chǎn)生。FR-008的生物合成起始單位的選擇是非常特異的。
應(yīng)用實(shí)例7預(yù)測(cè)FR-008分子中兩個(gè)順式雙鍵的存在聚酮合酶與動(dòng)物脂肪酸合成酶中的酮基還原酶結(jié)構(gòu)域組成了SDR還原酶總科中的一個(gè)亞科。聚酮合酶中酮基還原酶結(jié)構(gòu)域(KR)負(fù)責(zé)所合成碳鏈的立體構(gòu)型，而這種立體構(gòu)型的控制是通過酮基還原酶結(jié)構(gòu)域中一個(gè)特定位點(diǎn)的天冬氨酸來完成。這個(gè)天冬氨酸被認(rèn)為與酮基還原酶結(jié)構(gòu)域中112位的組氨酸和100位的酪氨酸共同控制所合成碳鏈的立體構(gòu)型。當(dāng)這個(gè)特定氨基酸是天冬氨酸(D)時(shí)，將酮基還原成D構(gòu)型的羥基；當(dāng)這個(gè)特定氨基酸是天冬氨酸以外的氨基酸時(shí)，將酮基還原成L構(gòu)型的羥基。在所有的酮基還原，脫水而形成反式雙鍵的反應(yīng)中，催化酮基還原的酮基還原酶結(jié)構(gòu)域都有這個(gè)特定的天冬氨酸。相反，在所有的酮基還原，脫水而形成順式雙鍵的反應(yīng)中，催化酮基還原的酮基還原酶結(jié)構(gòu)域都缺少這個(gè)特定的天冬氨酸或者這個(gè)特定的天冬氨酸改變。而且酮基還原酶結(jié)構(gòu)域這種對(duì)所合成碳鏈的立體構(gòu)型的控制不依賴于其它的酶或底物或酶促環(huán)境。FR-008聚酮合酶負(fù)責(zé)共扼雙鍵合成的聚酮合酶模塊8和模塊9中的酮基還原酶結(jié)構(gòu)域KR8和KR9的這個(gè)特定氨基酸分別是甘氨酸(G)和天冬酰氨(N)而不是天冬氨酸(D)(圖6)，所以它們所負(fù)責(zé)合成的雙鍵均為順式雙鍵。即FR-008分子中C28-C29和C30-C31之間的雙鍵是順式雙鍵。
應(yīng)用實(shí)例8預(yù)測(cè)FR-008分子中糖基附著的方向聚酮合酶中酮基還原酶結(jié)構(gòu)域(KR)負(fù)責(zé)所合成碳鏈的立體構(gòu)型，而這種立體構(gòu)型的控制通過酮基還原酶結(jié)構(gòu)域中一個(gè)特定位點(diǎn)的氨基酸來完成。當(dāng)這個(gè)特定氨基酸是天冬氨酸(D)時(shí)，將酮基還原成D構(gòu)型的羥基；當(dāng)這個(gè)特定氨基酸是天冬氨酸以外的氨基酸時(shí)，將酮基還原成L構(gòu)型的羥基。而且酮基還原酶結(jié)構(gòu)域這種對(duì)所合成碳鏈的立體構(gòu)型的控制不依賴于其它的酶或底物或酶促環(huán)境。FR-008聚酮合酶負(fù)責(zé)C-21位羥基形成的聚酮合酶模塊12中的酮基還原酶結(jié)構(gòu)域KR12的這個(gè)特定氨基酸是丙氨酸(A)而不是天冬氨酸(D)(圖6)，所以它所負(fù)責(zé)合成的C-21位羥基為L(zhǎng)構(gòu)型。而這個(gè)C-21位羥基是糖基化作用的位點(diǎn)，決定了糖基位于FR-008內(nèi)酯環(huán)的反面。其它攜帶氨基海藻糖的多烯大環(huán)內(nèi)酯的糖基也全部位于大環(huán)內(nèi)酯環(huán)的反面。
應(yīng)用實(shí)例9從鏈霉菌FR-008文庫(kù)中釣取新的攜帶FR-008生物合成基因的柯斯質(zhì)?？滤官|(zhì)粒pHZ194的序列測(cè)定發(fā)現(xiàn)它是一個(gè)重組的柯斯質(zhì)粒，有兩個(gè)載體pHZ132以及一個(gè)未知來源與FR-008生物合成無關(guān)的DNA片段。這可能是在文庫(kù)構(gòu)建過程中DNA重組的結(jié)果。
柯斯質(zhì)粒pHZ194的序列還未覆蓋完全的FR-008生物合成基因簇。為了從鏈霉菌FR-008文庫(kù)中釣取新的攜帶FR-008生物合成基因的柯斯質(zhì)粒，以柯斯質(zhì)粒pHZ194的最右端的與FR-008生物合成有關(guān)的序列(序列1，聚酮合酶序列)設(shè)計(jì)PCR(聚合酶鏈?zhǔn)椒磻?yīng))引物。
所設(shè)計(jì)的PCR引物序列如下CS1(5′-TGCCGCGCTCGCCGACA-3′)CS2(5′-CGCGTCCGGTGCTCACG-3′)其中CS1的序列是來自于本發(fā)明序列1中的118,479-118,463，CS2的序列是來自于本發(fā)明序列1中的118,262-118,278。以這兩個(gè)基于序列1設(shè)計(jì)的PCR引物，以柯斯質(zhì)粒pHZ194為底物，成功擴(kuò)增出了約200bp的DNA片段。
PCR條件如下0.1μg底物DNA，兩個(gè)引物各50pmol，2.5μl DMSO和25μl高保真PCR復(fù)合物(High Fidelity PCR Master，羅氏公司)，加無菌超純水到50μl。PCR儀是日本Takara Shuzo Co.公司的PCR Thermal Cycler MP TP3000。PCR反應(yīng)的循環(huán)條件為95℃(5分鐘)，30個(gè)循環(huán)的95℃(30秒)，65℃(30秒)和72℃(30秒)，最后是72℃7分鐘。
序列測(cè)定表明此約200bp DNA片段的序列確實(shí)來自于本發(fā)明序列1中的118,479-118,262共218bp的序列。
以此攜帶序列1中的118,479-118,262的218bp DNA片段為探針，與鏈霉菌FR-008基因文庫(kù)雜交，釣出兩個(gè)柯斯質(zhì)粒pJTU1和pJTU2。這兩個(gè)柯斯質(zhì)粒與pHZ194重疊并攜帶此218bp DNA片段。序列測(cè)定表明柯斯質(zhì)粒pJTU1(如圖1)攜帶了負(fù)責(zé)FR-008生物合成的基因?？滤官|(zhì)粒pJTU1外源片段的序列構(gòu)成了本發(fā)明序列1中的118,480-138,203的序列。
以下根據(jù)本發(fā)明的內(nèi)容提供基因序列(詳見下一頁(yè))。
SEQUENCE LISTING<110>上海交通大學(xué)韓國(guó)高等科學(xué)技術(shù)學(xué)院(Korea Advanced Institute of Science and Technology)<120>負(fù)責(zé)FR-008聚酮抗生素生物合成的基因簇<160>22<170>PatentIn version 3.1<210>1<211>138203<212>DNA<213>鏈霉菌FR-008<400>1ccaatggcga gatggccggg ggagtacggc atgagggccg gggggcacga tggggggcgg 60cgatcgtggc gggagggacc cagcgggggg gtcgtacctg ttccacgcgg ggcgcacggc120acggggcgcc tggtgggggg acgagacgtc cgtggggaag gaggcgtacg tcgaccgctt180caccgcgctg taggggctct cccgcgaaac ggcgcgagac cgagtgtttc agcggccgga240tcgacgcgga ccggctgacg cggctgctga ccctgttggg cgcccggccc ggccccggga300cccggttcgc ggtgtgcgga ccgtacggcc tggtggagac ggtccgctcc acgctcgacg360ggtggggggc cgagccggac accgtccgtt tcgagctgtt ctccaccgac ggggcaccgc420cggagccgga gcccgaggcg cccgccgtcc gcaggggccg ggtcaccacc gtgctcgccg480ggcgcaccgg cacggtgacg acggcaccgg aggacaccac gctcctcgac accgtgctgc540ggtgccgcgc ccactcacgt acgaggtcct cggttacctg gacttcaggc tgtgtccgcc600gaagccgctg cgccggtcgg agccgttgtt ctgctcctgc caccacgcgt cgaactcggc660ctggcccatc gcggaccagt cgggggtctc ctcgacctgg gcactgccca tccggcgggc720gagtcccacc atggccgtgc cgacgggacc ccgagccgcg tggtacccct ccaggatctc780cttccagctc tccccggcca cccaggcggc ttccagcgcg gtggcgtcct ggagtgcctt840cacactgccg gcgccgaggt gcggccgggc cacgctcgcg gcgtccccga tgagcaccat900ccggtcggtg gcgtagtgcg gcacgtccag gtcgtagatg ggctggatga aggtggtctc960ggccggggtg gagaggagct tggcggccca gaagggcggg aagtgctctg ccaccagggc 1020gcgcaggtgc tccgtcagtt ccgcgttgag ccggcccggc ggcagggagg tgggggtccg 1080caggtcgggg tggaggccgt cgatccgcgg gggtgccgtg tagagcaccc agttgaggcg 1140gtggccgccg gagccgtccg ggatgcggta ggccatgcag tggccgccgg ggaaggtgat 1200gttgtgcgcg tcgagcccgt ccgagggaag accggagacg tccggggagg tcccgcgcca 1260gccgatgtag ccggcgtacg cggcgccggt gcccgggtac atggcctcac ggacgacgga 1320gcggtagccg tcggcgccta tgaccaggtc gaagcgttcc ggctcgccgt cggcgagacg 1380taccgtgacc ccgtcggcgt cctgctccac cgcttccacc ttggcgcctg cccgataggt 1440ggcggtctcg ggcacccggc ggcgcaactc gctccacagc gaaccccagt tgtaggcccg 1500gaaggggaac ggctgggtgc cgatggctct gccgtgctcg gcgtcgccgt cgcgcacgct 1560ccacaccctg cggttcagcg gcgcccacgg catctcgtcc gccatgtaac cggcgtcgcg 1620
cagttcggcg taccgggcgt cgtggacacc gatgcccact cccctgtcgc ggagtcgcgc 1680gtcggcgcgt tcgaagacgg tgatcctctc cgccccgccg cgcgacgccg ccaatgcggc 1740ggcgcatccc gcgatactgc cgccgacgac ggcgacactg cctccacgca tactgactct 1800ccacttcttc gcgtgctggt gccctttccg gactttcccg caggggcgcg tcggaacggc 1860gatccgccgt ccccgacttg tccgcccttt cctgcggtga ggacacccgc gaaaccctat 1920cgaggtgggt gccgggcatg tcgagggcat tcgccggtcc gtcaggccgc cctggacgca 1980ggcgtatacg cggggcctgg cgcattcacc gttcccggcg cggaagggcc ggcctctccg 2040gaccgtacac acggttcgcg acgccgcgat gcacacgaga tcatttcttc ccgcatccgc 2100tttccccaca tgccgtgcag ggaaatgtcc gggtgctgcc gaaaaggaga gcgggtgggc 2160gaatggcgaa tgccgggcgg catggaggaa atgggcgatc gaccgtccgc ccgaggactc 2220ctgcgcgagc ggcggaacgg agcgccccgg cgccgccgcc ccgtcagagc ggggagccgg 2280gaagtgcctg gtagatctcc gcgagccggg tcaccgagtg atgggccgcc gggaactcac 2340ggtcgtcgat gccggtgacg ggacggaccc cgacgcggcg ttggtcgcga agaccgcctc 2400gactgagtcg aggtccgcga ggccgaccag ttcggtggga ccgtcggtgg ccccgccgca 2460gcagctgccg ggtcgtgccg gccagcacct ccccgccggg ccagaccacc tcgccgtcgc 2520gcaccaggcc gacgttccag gtgccgccct ccaggatgtc gccgtcgggg cccgtgaaca 2580gcacgtcgtc gtaacccgcc cgttgggcct gcctgcgcag ccgcagggtg ggacagaggc 2640cgaccgactt cacctcggga aggtcgcggc ggtggaccac cgaacgt1cc cgcagcggac 2700cagggggctt gtcgggggcg ggccgggagg tcaccaggat gcccggccgg gcgtcggcgg 2760cgatgttccc caggttcagc gccgggtcga agacggtgac acggacggtg gcccggccgt 2820ctgttggtgc cgcccggcgg gcgagcttcc gcacccggtc cgggtcgagc gccgcgtcga 2880agagggtccg gcagtcgcgg atgaggcgtt ccaggtggag gtcgagcccg cgcacccggc 2940cgttctcgac gagcagcgtg gtgaaatggc cgtagttggt gagggcgagc gaggccagcg 3000cctcgggccc ggcgggttcc ccgtccagtt cgatcatgac gtcaccctgt cactgtgacc 3060gccgtaccgt gcgcggagcg cgcgacgggg acgagcgggg gtgcggaccg gcggccgggc 3120tgccgccggc ggggcacacg gtgtacggat cacttgatga agtcctcgac gaccttcggc 3180ggccaggtgc cgaccttgag gacgcccatg gagtaggcgc gggagacgag agcggcacgg 3240ttgggcacct tgagcttccg cagcagcccg gtgacgtggt actccacgcc ctggcggctg 3300aggtagaggc gggaggcgag cgggatggtc gagaccccgg cggctatgcc ctccaggatg 3360cgtgcgtcga tctcgctgag gagcttcttg cggggcgcca tgacgtccgc gtccccggcc 3420tcctcctccg cggcgttcat caccacgagg atcgcggcca cgtcgggcgt acggccaccg 3480cgtaccgcga gcgcgttcag cggcagcgtg gacgccgtgc gttcctggtc gaccgcgatg 3540acctcggtgg cgaagcggtg ccgcttgccg tccagcatcc gcgcgaactg gtgcatgagg 3600ggctgctgga cgctcgggtg gacgaggtcg cagaagcggc tgccgcagag ttcggaggac 3660gagccgccga agcggcgctc gaactcctgg ttgacctgct ggatcgtgag agacgggtcg 3720aggcaggcga cataggcacc ggcccggtcg aacccgtccg cgagaacggc ggcccgttcc 3780ggcgagccgg ccgggtcggc cgccggcgcg ggatccatga gcgtgctgtg gggggtggcg 3840ggcatcaagg aactcataac ctttcatcgt ttcgcattac gggggtgggg tggcgcggcc 3900cgcgctcagg aaaaggcgac cgcgcccagg gcccttgagt aagggccagc gacgagaaag 3960aagatgtttt ggatcctacg aaagcggcta tcggcggtca acgcggtcca gggctgaccg 4020gccaatactg tggtagcccc ttcggtaggt acggtcccgt accgaagagg ttcgggcggg 4080gagggttcgg tgtgcgtacc tcaccgcccc gcaccgcgtc cacctgcgaa gaagggtcga 4140actcgggcgc cgttccccgc cccgggggcc ggacggccct agccgtcccc gcaccgtggc 4200cccgcacacc gcggaaatgg tgacgaacgt tcatccagat ttcccggccc ggggcgcaaa 4260
cgacattctg ggggacgccg gatgtcctgg ccgcaccatg gccggaaaac aaggacgccc 4320ggggtccttc cgcgaatatg cggcggaagg agcccgggcg tttccctcgg cgcgtatcag 4380tcgccggtgc cggcgtattc cgcgcgggag aagaggacga tcagttcccg gcggccggag 4440atgcccaggc gccggtaggc gtgggtcagg tgcttctcca cggcgcgcga gctgaccccg 4500agggcgccgg cgatctcctg gttggtcagc ccccggcagg ccagccccac cacgcggcgc 4560tcggtcgggg tgagcacgtc ctccggtgtg gccggcgggc cggagcgatg accggcaccc 4620cgggccgccc gttcggccag cccgggggcg ccgcacgagg tggccagggt ggcggcctcc 4680cgggcgagcg cctcggtctc ggcgccgccg cccaggcgtt cggcgagggt ggacagggcg 4740cgggccagtt cgagctggtt ggcggagcca cgcagcacgg tgacggcctc gcgcagcaga 4800ccgatgcccc ggccgccgtc ctccaggcgg ccgagcagcc gcagcgcgcg gcccaccccg 4860acgggggcgc cccactgctc ggcccgcgcg tactcctcct cggcgaggct ccgggcggtg 4920cggatgtcac ccaggcggcg gtggacgccg acggcccagg ggcgccaggg gaagagcacc 4980gggttgtgcc agccggcggc ttcgagctgg cggccgcagg agtgcagcga gtcgagggct 5040ccggtctggt cgcccgcggc gatctcccgg gtggcctcca gcaggcgcag gatcccggtg 5100agggtcaggc cctgcggcct gatgcggctc gcgcgccgca gcaccttctc ggcgagcgag 5160gggtcgcggg actccagcgc gacgatcgcg tgcgtcatcg tcgccgcggt gtccaggccc 5220tggtgggcgg gctccgtcat cccgagggcc gtctcggact gctcgcgggc ctgggcgagc 5280cggccgcgcc cgaggtggac gagggcctgc tcgatgtgga ccagggcgtt ggcgggggtg 5340gtaccccgct cgttggcacg gtgctcgacg gcgagccagg agtcgacggc ctccaccgag 5400tcggcggcga tcatcgtgat gatcgccagc gggagggtgg agtggatgcg ggcggaggtg 5460gcgggctcgc ggtccaggac gcgcccggcg aggcgcacca cctcggaggc gggcaggtga 5520cagccgaggg tcgcggcacc gagcagcgcg atcgtcagct cccgctcgcc tccggtgtcc 5580accgggggct cggggcccag ttccttcaac cggtgcgccg aggcggcgag ttcgtggggg 5640tcctcgtggc cgcagtggcg cagcctggct tccagacgga gcgcgagatc gcggtcggtg 5700ccggtgagcc cgtcggcggg cccgaggtcc tcggcggcct ggcgcagcag gtcgacggcg 5760gtgagcgggg cggggcccag cgcggtcggg gagatgcgca gggcggcgac ggcccggtcc 5820cgtggttcgg ggagcagggt catggcctgg gcgatgtggc gttcgcaggc gagtgggtcg 5880aaccctctct ccgcggtgcc cagttcgacg aggaggcggc cgcggccgac gccggcggcg 5940gggctgtcca gcagggcgcg gcacaggtag ccggcggcgg tgtcgggggc gccgcggcgc 6000agcgcggtgt cggcggcggc gcgcaggacg tcgacggccc acggctggcg ccgggtgacg 6060acggccatga gctgggcggc gacctgttcg gccggggagc cggcggcgta gaggagggcg 6120gcggcctcgt cgtgcatccg ctcccgctgg accatggtga gggtggactc ggcggcgtcg 6180cggacggacc ggtggatgaa gcgcggttcg tcgggggcgg cgagggcgcc gagcgcgccg 6240agggcgcgca gggcccccgc gtagccgatc gagtcgagcc cggccagccg ggccagggtg 6300ggcgcgtcgc tctgttcgcc gaggatggcg atggcggcgg cgaggtcgcg gaccggggcc 6360ggctgggtgc gcaggatgct ggcgagccgt tcgcgcagtt gggaagggcg cagccggcgt 6420gcggtctcgg cgtgttcggc gagcgggcgg cggccgagga agccggtgcc gacgaggatc 6480gacagcagga agagcgggtt gccgacggac gcctcgtggc aggcctggac gaactcgtcg 6540tcgcccgcct cgccgaagtg ctcccggacc agctcgtggg tggccgtcag gctgagggag 6600gcggggcgca gggtttgggt gccggcctct cggatctcgc gggcgagggt gtggtggctg 6660cgggggtccc cgtcgcgcag ggcgcagacg acgacggccc gcagcccgtg cagccgcttg 6720gcgaggtagg tgagccagcg cagcgagggc acgtcggacc actgcaggtc gtcgacgagg 6780atgagcaggc ggctgtcggc gctcacgttg gcgagcagcg agagcagtcc gtgcagcacg 6840gcctcggtgg cggcgatggc ctggtcggcg ccgggcgggg cggcgtcgtc ggccaggacg 6900
tggcgggcga aggaggcgtg cgcgtccatc cagcggtccc gggcgtcctc gggcgagtcg 6960ccgagcagcg tctcgaagag ctgccgtacg actccgaagg cgaagtcctg ttccatgggg 7020gccgcgtggg cccgcagcac ccggacgtcg tcccggtcgt cggccaggca ggacagccgc 7080tgcaggaggg cggagcggcc gatgccgagg gggccggtga ggaggacgag ggaggacctg 7140ccttcggtcg cggcgcccag ggcggcgtcg accagggcga tctcggtggt gcgctccagc 7200aacatgcgat cagctgccct ccgcttcttg tacgagtgcg cgcagttcct cacgtccgcg 7260gatgcggagt ttgcggtaga cgccgctgag gcgcagctcc acggcgcgcg tggtcacggc 7320gagttccgtg gcgatctccc ggttgcccag gccctgggcg gcgagggccg cggtctcccg 7380ttcgacggcg gtcaggccgt gccagacggc cgcgtcgtgg cccggggcct tcgcggtccg 7440ccgaccggtc agcaccgccg ggcgcgggcc ggggggcctg gccaccgcgg aggggacgtg 7500cggaccgggg ggtggggcga ccggcgggag caccgtggcg gccgggccga gggcgacggc 7560ggtccggcgc tcgttctcct cgcgttcggc ggcggccagt tcgatgacgg cccgggtgta 7620ggcggtgcgg gccgatgccg cgctgagcgt ggtgacggcg gcacggaggt tttcgacccg 7680gttctcctcg gtgacgacgg cgaggctgag ctgggcgcgg ccgagcgggg cggcggcgcc 7740ccagcggcgg gcgaggcgga cttcctcgtg gaccagccgg gcggcctcct cggtgtcgcc 7800gagggtgtgg gcggcctcgg cggcgaggga gcgccagggc tggacggccg ggttggcgca 7860gccgtgccgc aggagccagc gaccgcaggc gcggaagtgt tccctggcct ggtggggctc 7920gtcgtccgtg cgggccagca ccccccgggc gaagagcagt tgggcggtgg tggcgctctc 7980gtgggcgagc ggcggcagcg gccgggcggc ggtctcgcgg gcccgttcgg tgtggccgtt 8040ctgcagatcg gtgatcatgc tcagggcgag ccagtacggg aggaagagcg ggtggaggcg 8100gtccagcggg agttccgcct gggcggcggc caggtcacgg gcggcggcgt cggggcggcc 8160gtggcggatg tgcagctcgg cgcggacggt gaggatgtgg gcgaccgaga tcgccgcgcg 8220ctcccggtgg gactcgtcga gcagggcacc gaggtggttc tccgcctcgt cgccgtcgtc 8280ggtgagcagg agggcgcggc agccgagcag ccggggtacg acgaggagcg ggcggccggt 8340ggcggcgggg acgaaggcgg agcgggcgag tctgcgtact tcctcgcggt ggacgccggc 8400cacgccgtgt tcccaggccc agacccccgc gacggccggg ttgtgcaccg cgtcgggcag 8460ggcggggacg gggaggacgt cgaggtcggc gtcgtcgtgg cggaggaagg aggcgacgcg 8520gcacaggacg gtcagttcgt gccgttcggc gacggccggg tcctcgtccg gcccggcggt 8580gtccggtcgt cctgcggcct cggggccggg agagcggggg gcgtcgtggg cgcggggggt 8640gccgggaagg tccggcgcgg cggatcttct accgtccggc ccggtggcgc cgggcggggt 8700gcccgcgtag gggcggtgag ggacgaggtg ggtgcgggga gtcgaggcgg gctccgcctc 8760gggcggcacg gccggggcgc cggtcgggcc acgtcgtggt tcgggggcgg ccggggccga 8820gtcgatggct ccggcgaggg cgtggcgggc cgcgcgggtg tctccgccga ggaggcacag 8880gtcggtggcg cgggcccgca gtccggcgcc ggggccgggc cgggtggccc ggatgagccc 8940gcccagccgg cgttcggcgg ccgtgggggc ggtgaccatc tccacggagg ccagctggaa 9000ctcgatccgg gcgcggtcct cggcggccag tggttcgtcc agggcgcgcg ccaggtaggc 9060gacggccagg tcgcggcggc cgccgcgcag cgcggaggtg aagccccgcc gcagggtgtc 9120gacggcccag gggtcgccga cggggcgggc cagcagcagc aggtcggcta tgccctggtc 9180gtcggcggcg acgcgctggg cgagcccggc ggcgcgtgcg tacagctcgg cccggtccgc 9240tgcgggcatc tcctccagga cgcgggcgcg tacgacggcg tcctggaccc gggggtggcc 9300gtcgcgcagg gtggtcaggc cgctggccgc cagggcggcg cgcaggcggg actcggagac 9360ggagtgcggt ccggcgagcg tgcagacgag ggggaagtcg aggaggtcgc cgcagacggc 9420gagggcgcgc agggcgtcga cggcggccgg gtcgcgcagg tcgctgaggg cgcgggtggc 9480gtggtcgccg acgacctccg cggtcagggc gcgcaggggg tcgatccggt cggcccgggg 9540
gctgtacccc gcgcgggcga aacggtccag cacgtcgtgg acgacggccg ggatgccccg 9600ggtcgcctcg gcgagggcct cggtgaaggc ggggtccccc ggggcgccga aggcccgcag 9660gacggcggtg gcggtgccgc cggaggtgag gggcggcagc gccagctcga tggtggtggc 9720cagtccggtc aggggggtgc cgacgctcca ctcgggccgg gtcagggcgg tgccggtggt 9780gctggtgagc agggcgaccg gggcgcgggg cagccgccgg gcgagggctt ccagccagcg 9840cagggaggcc gggtcgagcc actgggtgtc gcgtaccacg agcagggtgg gccggtcgcg 9900gacggcgttg agcaggccgt tgaggcccgg cagcccgccg ggctgctcgt cggtgaggaa 9960gaggcgcggg gcgagtgcgc ggttctcctc cgcgaggagc tgggccacca cgccgtaccg 10020cacctcgtgt tcggcggggg tggcgtgggc gctgaggacg cgcagtccgg cgtcggtggc 10080gtacgcggcc gcccagcgca gcagatcgtt ctggccgagc cccggttctc cggtcaccgt 10140caccaccgcg ggacggccgg atccgagctc gccgatgacg gcggagaggg cggccagttc 10200gcggtcgcgc tccaggagcg ggagctcgat gggcggcatg tgaagatcat acatttgcgc 10260agtgcagcag gtgggcgtct gtaacccact gttcttccta tggatttctc cgcttcgcat 10320gtcccggccg cctctccgca gccggccggg cggtggcgcc ggcctcgcgc agggcctcgg 10380tcagatcgcc ccggcgggcg actcccagtt tccggtagac gctggtgagg tggagttcca 10440cggtccgcac ggtgacgaag agggattccg ccacttcgcg gttgccggcg cccgaggcga 10500ccagcccggc cacggtgcgc tcggtgccgg tgagcatgtc cagcggtgag gcggtgacct 10560cgcgcatccg tccgccggcc gcgatcagct cgtcgcgggc gcgccgggcg agggcgacgc 10620acccgcaccg ccgggccagc ccgacggcct cccgtagatg ctcccggccc tcccgcacgg 10680ccccgtcctc caccagggcg cgtcccagca gcaacaggcc gcgggcgtgc ccgctccgcg 10740cgggcgagtc cgccagcagg gcgaccgcct cccgcagcgc cccggtgcgc gccgtgccgc 10800gcgccgccgc gccccgggcg agcgcggcgt accccagggc ccggcgggtg ccccagcgtt 10860cggcgagccg ggtgccgtgc gcggcggccc gccccgcctc ctcgccgcgt ccggcctcgc 10920ccagcagaca ggccgtctcc agccaccacg gcgcgaggac cgggttggtg agtccggcct 10980ccgccatcga ggcgccgcag gacccgaaga cctgcagggc tccgtccagg tcgccgtcgg 11040cggcaagcgc ccggccacgc gccatcaggt accagtggta ctcccagacg aaccggtcca 11100ggtgggggcg tttgatggcg tccagcgcct ccagcgcgcg ccccggatca ccccgctcgg 11160tgaggacgga ggcgtacgcc gtgtgcggca tcgtggcggt gtcgccccag cgttcctggc 11220cgaggatctc cagcgcggtc tgcgcgtcgg ccatcgcgtc cggcaccgcc ccgttctcca 11280ggcggaacag cgaccgggtg gagagcgcga gcacgtacgt ccagaccgcg gcggtgtcgc 11340cgctgccgcg cagcacggtc tccaggacct cctcggcggc ctcgttctcg tcggccagcg 11400acagggcgag cgaggtgggc agcagtgacc agacgcccag cggcaccccg ggggcgcgca 11460gcgcgcggcg ggcccggccc acggtctcct ccgctccccc gccctcggcg gcggagagca 11520cactcagcat ggcgagttgc tgacgctggg cgggggtgtc ccccggctgc ggggtgaggc 11580cctcggtccg gcgcaggatg tcgggcaggg tggccttctc gtcggagccc acgatcagca 11640gggccgactc cgtgagggtg cgcagttcgc ggtcggcggg ttcgggctcg ggacccagct 11700cggcgtcgag ggcgtccagc gcctcggtga gggcccgggc accgtccggc gactgctgca 11760cggcgaggca ggtcaggccc agctgaaccg ctgtggcggc gcgggcgcgg acgtccgtgg 11820tcagggagtg ggcctcgtgc agcagggtca ccgagcgggc cgggtcggtc tcggccagcg 11880ccttgccgag ccgggagagg acgtcggggt ccttcggctc ggcctcgcgg acgcgctcca 11940ggtagcgggc ggcggcctca caggcgccgc gctgttcggc ctgggcggcg gcgtcccgga 12000gcacggagac cgcccagtcg tcgggggtgc ccgggaccag cagcagctgc gtggcgatct 12060cctcggggga ccgtccgacg tcgctgagga gccgggcggc gcgtctgcgc aggacggcga 12120cgcccgacgc gccgacggcg tcgaggaccg cggcgcgcac caggtcgtgg gagagctcca 12180
ggtggccggg gtggatcagc ccggcgtcgc gcaggatctc cacggcctcc tccacgtgga 12240cgacggagac cctgccgagg gcggccaggt acgtccggtc ctcgtcgccg agcacggcca 12300cggcacgcgc gacctggcgc acccagtccg gctggtgttc cagcacaccc cggacggagg 12360cggcgacgac cttcccgccg gcctgggcga ggcgtgccgt cccggtgtcg tcggggccca 12420gtccggcgga gcgcaggtcg cgcaggagcc ggacgatctc cagcgggctg ccgccggtca 12480cggagaggag gcggccgacg aacgacggca gggggtcctg ctccgggaag gagtgggcgg 12540tcagctccgc cacctcggcg gtgtccagcg ggccgagccc gagtgaggcc ggcaggtggt 12600gggcgaccag gtcggcgagg gcgtcgggag cggtgaggcc ggtgccggtg cggtgggcgg 12660cgacgacgag cagcggcagg ccgtccgcgc ggcgcagcag gaagtcgagc cagcgcaggg 12720accgctcgtc gcaccagtgg acgtcgtcga ggacgagcac cagggggccg tccgccatga 12780ggttggccgc gagccagtac aggccctgga ggacggagaa ggtgctgccg gggtcggcgt 12840ccagctcccc cgggtcggcg gcgagggcgg gcagcgcgcg ccgggcgccg ccgacaagga 12900gcggcgaggt acggcccttg cggccggtca ggccgagtcc gccgaacagg gcgcggaccc 12960cgctgtagcc cgtggcgacg tcgacggcgc ggcaggagcc gtacagggtg gtcatgccgg 13020cgagggggcc gtcctcggcc agggcggcgc gcagcagtgc ggtcttgccg attccggcgg 13080gaccgtccag gagcaccagg cggggtcttc ccgcgcgggc ggcgtccgcg tgccgggcga 13140ggacgtcgag ctggtgggcc ctgccgacga ggggcggggc cgggggcacg gcacgggacc 13200ggtgaggcac agggctctcc ctcgcgtcgg acggggccag ggacggcggc ccggcggaac 13260cggaacggcc cggggcggcg ccccgggacc ggtcacctgc ccaccggagc cacatctacg 13320cacacccggg tggccggcgc agcggtccgg cgccgaatct aaggatttcc ccggtgccgg 13380acgtgagatc aagtggtggt gcggtcgggg ccgcccctgt ccggcccggc cggcgggcgg 13440catccgccac cgccccgcgc ccgtccgcgc gtgaccggcg ggccgcgacg actgtcgccg 13500tcacttcgtg aaagggctcc catggactcc gccgcccggc cgatcctctt cgtcagtctc 13560cccgagagcg ggctgctcaa cccgttgctg gtgctggccg gggagcttgc ccggcgcggt 13620gtgccggacc tgtggttcgc caccgacgag caccggcgcg aggaggtgga ggcgctctcc 13680gacgtctcca aggtgtcgtt cgcctcgctg ggcgaggtgg tccccgaact gtcggcggtg 13740acctgggacg acgaggtgta ccgcgaggtc acccagtcct cccgcttcaa ggcccaccgc 13800gcggtcgtcc ggcagtccta ccggcccgcg ctccaggcgc gcaagtaccg cgagctggag 13860caggtcgtcg acgaggtccg gccggcgctg atggtcgtcg actgcgtcgc gggcttcggc 13920gtcgacctgg ccctggcccg gggcatcccg tacgtgctga acgtgccgtt cgtcgccagc 13980aacgtgctga cctcgcacaa cccgttcggc gcctcgtaca cccccaagag cttcccggtg 14040cccaactcgg ggctgcccgc gcggatgtcg gtgcggcaga agctggccaa cacgctgttc 14100aagtggcgga cgctcggcat gttcctgcac ccggacatgg cggcactgct gcgcgaggac 14160gccgcgatac gcaaggaact gggcatcgcc ccgccgaacg ccatgacccg ggtcgacgag 14220gccgccgccg tggtgtgctc ctccgtcgcg gaactggact accccttcga catcccggac 14280cgggtgagcc tggtgggcgc cgtgctgccg ccgctgcccg aggcaccgga cgacgacgag 14340gtcaccaggt ggctcgacgc gcagtcgtcg gtggtctaca tgggcttcgg caccatcacg 14400cgcctcaccc gcgaggaggt cgcggcgctg gtggaggtgg cccggcggat gtccggcacc 14460caccagttcc tgtggaagct gcccaaggag cagcagcacc tgctgcccga ggccgggtcg 14520ctgccggaca acctgcgggt ggagagctgg gtgccctcgc agctcgatgt gctggcccac 14580ccgaacgtct ccgtcttctt ctcgcacggc ggcggcaacg cctaccacga gggcgtctac 14640ttcggtaagc cgcaggtcgt gcggcccctg tgggtggact gcttcgacca ggcggtccgc 14700ggccgcgact tcggcatcag cctgaccctg gacaagccgc acaccgtcga ccccgacgac 14760gtggtggaca agctgacccg ggtcacctcc gaccccgcct tccgtaccga ggccgaacgc 14820
ctgggcgccc tgcagcgggc ggccggaggc cgtgcggccg ccgccgatct cgtgaccggc 14880ctgctcccgg cggcatgaca gcggccgcgc ccgaggccgc ctgacccatc gaccgacagc 14940agcgaggacc ccatggcctt cacccatccc gtctcccggc ccgctctgga cggccgggaa 15000ctggagtacg tctccgacgc cgtctccggc ggctggatct cctcccaggg accgtacgtc 15060cggcgtttcg aggaagcctt cgccgagtgg aacggcgtgg cgcacggggt ggcctgctcc 15120tccggcaccg ccgccctcac cctggcgctg cgcgcgctga acatcggccc gggtgacgag 15180gtgatcgtgc cggagttcac gatggtcgcc tccgcgtggg cggtgaccta caccggcgcc 15240accccggtct tcgtggactg cggcgacgac ctgaacatcg acgtcacccg catcgaggag 15300aagatcactc cgcgcacccg ggcggtcatg ccggtgcacg tctacggccg gcgctgcgac 15360atggacgccg tgatggacct cgccctgcag tacaacctgc gggtggtcga ggactcggcg 15420gaggcgcacg gggtccgccc ggtcggcgac atcgcctgct tctcgctctt cgccaacaag 15480atcatcacgg ccggcgaggg aggcgtctgc ctcaccgacg atccccggct cgccgaacag 15540ctggcccatc tgcgggcgat ggccttcacc cgcgaccaca gcttcctgca caagaaactg 15600gcctacaact accggatgac ggccatgcag ggcgccgtcg ccctggccca gaccgagcgg 15660ctcgacgaga tcctcgccac ccgccgggag atcgaggccc gctacgacgc ggggctcaag 15720gacctccccg gcatcaccct gatgcctgcc cgcgacgtcc tgtggatgta cgacctgcgt 15780gccgagcgcc gcgaggaact ccgcgcccac ctggacgcac gcggcatcga gacccggctg 15840ttcttcaagc cgatgagccg ccagcccggc tacctcgacc cggtgtggcc gacgctcaac 15900gcccaccggt tcagcgagga cggcctgtac ctgcccaccc acaccgggct gaccgccgcc 15960gaccaggagt acatcaccgg cgccgtccgg gacttctacc gcgcgggctg accgacggcc 16020cgtgcagcgg gccgcgcaga ccgcccgccg ccggcccgac cgggcgcggc accagccgac 16080gcaggaagag aaacgaggag cggacatgac gaccagcccc ggcccgacgg tggtcgactt 16140cccacggcgc acgccccggg aaccgctgcc gctgtcccag tacgccgaac accggaagca 16200gaacggcctg gtccagacgc acctgccgaa cggccggccg atctggctgg tgacccggca 16260cgaggacgtg cgcgcggtcc tcacccatcc ccggatcagc gccaaccccg acaacgaggg 16320cttccccaac gtcggcgaga cgatgggggt gcccaagcag gagcagatcc ccggctggtt 16380cgtcggtctg gactcccccg agcacgaccg tttccgcaag gtgctgatcc ccgagttcac 16440cgtgcgccgc gtccgtgagc tgcggccggc gatcgaacgg accgtggacg agcggatcga 16500cgcgatgctg gccggcggga acaccgcgga cctggtgaac gacttcgcgc tgcccgtgcc 16560gtccctggtc atctccgcgc tgctgggggt gccgtcggcg gaccgggact tcttcgagtc 16620ccgcacccgc acgctggtcg ccatccgcac ctcgaccgac gaggagcgcg ccgaggccac 16680ccggcagctc ctgcggtaca tcaaccggct catcgtcatc aagaagaagt ggcgcggcga 16740ggacctcatc agccgcctgc tgtcgaccgg caagctctcg gacgaggagc tgtcgggcgt 16800cctgctgctc ctgctgatcg cgggccacga gaccacggcg aacaacatcg ggctcggcgt 16860cgtcacgctg ctctcgcacc gggagtggat cggcgacgac cggctcgtgg aggagctgct 16920gcgcctgcac tcggtggccg acatggtggc gctgcgcgtc gccgtggacg acgtggagat 16980cgccggccag accatccgca agggcgaggg catcgtgccg ctgctcgcct ccgccaacca 17040cgacaccgag gcgttcggct gcccgcacgc cttcaacccg gagcgcaccg agcgccgtca 17100cgtggcgttc ggctacggcg tccaccagtg cctgggccag aacctggtcc gggtcgagat 17160ggagatcgcc taccgcaagc tcttcgagcg catcccggag ctgcggctgg ccgtgcccga 17220ggaccagctc gcctacaagt acgacgggat cctgttcggg ctgcacgagc tgccggtgcg 17280ctggtagccg ggcgccgccg acggaccccc catccaccga ggagccaacg atcatgcgtg 17340tcaccgtcga cagcgagcag tgcgtaggag cgggccagtg cgtcctgaac gcgccggagg 17400tcttcgacca ggacgacgac ggcgtcgtgg tcctgctccg cgcggagccc gacgagcggg 17460
accacgaggc ggtccgcacg gcgggcgacc tgtgcccgtc ggcctcggtc gtcctccagg 17520aggactgaac cagccggctg cgcggaccgg caccggtgcc ccgtccggcc cggccctcgc 17580acccgcggac ccgtcgccgg cggaacaccg accggcgcca gaaccatcga agggactccc 17640ccgtgaccac cgctgacgac accgcggccc gctggctgcg gcggtaccac ccggccgagg 17700ccgacgcggt acggctggtg tgcttcccgc acgccggcgg ctccgccagc ttctaccacc 17760cggtctcggc gcggttcgcg ccgggcgccg aggtcgtctc gctccagtac ccgggccggc 17820aggaccgccg caaggagccc tgtgtcccgg acctcggcac gctggccgac ctgatcaccg 17880agcagctgct cccgctggac gagcggccca ccgtcttctt cgggcacagc atgggggccg 17940cgctcgcctt cgagacggcg tggcggctgg agcagaaggg cgccggtccc cgcaccgtca 18000tcgcctccgg acggcgcggc ccctccacca cccgcgccga acgggtccac acgagggacg 18060acgacgggat cgtcgcggag atgaagcggc tgaacggcac ggcggccggt gtcctcggcg 18120acgaggagat cctccgcatg gcgctgcccg cgctgcgcgg cgactaccgc gccatcgaga 18180cctacacctg ccctccggac cgccggctgg cctgcgggct gaccgtgctg acgggcgagg 18240acgacccgct gaccaccgtc gaggaggcgg agcggtggcg cgaccacacc accgggccgt 18300tccggctgcg ggtcttcacg ggcgggcact tcttcctcac ccagcacctc gacgcggtca 18360acacggagat cgcccaggcc ctccaccccg accgggccgc cccggccgcc tgagcgtccg 18420gcccggccgg cggcgggacc gccggccggg caggcgctgg agccgtgacc gacgcaccgc 18480cgacggcgta cgcgcgtacg cggcggacgt cgtccgcacc accggagagc ccgcacaggc 18540ccggacggcg atcccgtccg accgcctccc ccgtcacttt ccgctcacgc gcctgaggga 18600caaggacaca tgcgcaccct tctcgtcgac aactacgact cgttcaccta caacctcttc 18660cactacctct cccgggccaa cggccgggaa cccgaggtca tccgcaacga cgacccggcc 18720tggcggcccg gtctgctcga cgcgttcgac aacgtggtgc tctccccggg gccgggtacc 18780ccacaccgcc cggccgactt cggcctgtgc gcccggatcg ccgaggaggg ccggctgccg 18840gtgctcggcg tctgcctggg ccaccagggc atggccctcg cccacggcgc ccgggtgggc 18900cgggcccccg agccccgtca cggccgcacc tcggcggtac ggcacgacgg caccgggctc 18960ttcgaggggc tgccgcagcc gctggaggtg gtgcggtacc actccctcgc ggtgacggaa 19020ctgccgccgg agctggaggc caccgcctgg tcggaggacg gggtcctgat ggcgctgcgc 19080caccgcacgc tgccgctgtg gggggtgcag ttccaccccg agtcgatcgg cacccaggac 19140ggccaccggc tgctggccaa cttccgcgac ctcaccgagc gccacggccg aacgcgcccg 19200ggcggccggg cggggcacgg cacgctcccg ccccccgcgc ccgcccggga gacgacggcc 19260accaccggca cgccacggcg gctccgggtc atcgccgagt cgctgcccac gcgctgggac 19320gccgaggtcg ccttcgactc gctgttccgc accggcgacc accccttctg gctcgacagc 19380agccgtcccg ggggcgagct gggccagctc tccgtgatgg gcgacgcctc aggtcccctc 19440gcccggaccg ccaaggccga cgtgcacgcc ggaacggtca cggtgagagc cgacggcgcc 19500agcagcacgg tcgagagcgc cttcctgacc tggctggaga acgacctggc ggggctgcgc 19560accgaggtgc ccgaacttcc cttcgcgttc gcgctcggct gggtcggctg cctgggctac 19620gagttgaagg ccgagtgcga cggcgacgcc gcgcaccgct cacccgatcc cgacgccgtg 19680ctggtcttcg ccgaccgggc cctggtgctg gaccaccgca cccgcaccac ctacctgctg 19740gcgctggtgg aggacgacgc cgaggccgag gcacgcgcct ggctcgcggc ggcctccgcc 19800accctggagg ccatcgccgg gcgggagccc gagccgtgcc ccgaggcgcc cgtgtgcacg 19860acgggtccgg tggagctgcg ccacgaccgg gacggctacc tgaagctgat cgacgtctgc 19920cagcaggaga tagccgccgg ggagacctac gaggtctgcc tgaccaacat ggccgaggcg 19980gacaccgacc tcaccccgtg ggcggcctac cgcgcgctgc gccgggtgag ccccgccccg 20040ttcgccgcgt tcctggactt cggccccatg gccgtgctca gcagctctcc ggagcggttc 20100
ctgcgcatcg accggcacgg gcggatggag tccaagccga tcaagggcac gcggccacgc 20160ggcgccaccc cgcaggagga cgccgcgctc gtacgcgccc tggccacctg cgagaaggac 20220cgcgccgaga acctgatgat cgtcgacctg gtccgccacg acctggggcg gtgcgccgag 20280gtcggctcgg tcgtcgccga cccggtgttc caggtcgaga cgtacgcgac cgtgcaccag 20340ctggtcagta ccgtcacggc gcggctgcgc gaggacagca gcccggtggc ggcggtccgg 20400gcggccttcc ccggcggatc gatgaccggg gcgccgaaga tccgcaccat gcagatcatc 20460gaccggctgg aaggcgggcc gcgcggtgtc tactcgggcg ccatcggcta cttctccctc 20520accggcgcgg tggacctgtc catcgtgatc cgcacggtgg tgctcagcgg cggcaggctg 20580cgctacggcg tcggcggcgc cgtcatcgcg ctctccgacc cggccgacga gttcgaggag 20640acggcggtca aggccgcccc gctgctgcgt ctcctcgaca ccgccttccc gggccgtgag 20700gctcccggca aggacctcga cggggaaccc gacgacggca cggacgcggg tgctccgaag 20760gacctcgtcc tgcccgggtg acctcacggg tgccccggcg gtccggcggg ccgccggggc 20820cccgcgcggc cggcaccggc cgcatctcta gggaaaacac gggccccgtg gcaagagcct 20880ggaaccgttc ccccctctga cggcgacgct cgacatcccg ggagtgatgg tgcccgtcca 20940cgcacacgac tacgtgaccg atccgccctc caccaccggc aggaccctgg acgggctgac 21000gctgccacgg gtgttcgccg acgccgtaca ccggggcggc gacgccgtgg ccctggtgga 21060cggggagtac gccctgacct ggagcgcctg gcggacggcg gtggacgcgc tggcgcgcgg 21120cctccaggag tccggcgtcg tctccggcga cgtggtggcc ctgcacctgc ccaacagctg 21180ggagtacctg acgctccatc tggccgccgc ctcggtcggg gcggtcacga tgccggtgca 21240ccagggcaac gctccctcgg acgtccgggc cctgctggaa cgggtccggc ccgcagccgt 21300cgtcctgccg gcgcggaccc aggagggtgg gggcccgctc accggcacgg cgctgcgcga 21360ggtcctgccc gagctgcgcg ccgtgctcgt cacgggcgac gcggcgggcg agggcaccga 21420gacggtgacc gagatgctgg agcggtggtc cggagaggac ccgctgcccg tcgaggtgcg 21480cccggactcg ccgttcctgc tgctgccgtc ctcgggcacc acctcggcgc ggcccaagat 21540ctgcctccac tcgcacgagg ggctgctcac caactcccgg gccgccaccg aggacaccgc 21600ggacgcctac gccggcaccc tgatcaccgc gtgccccctg acccactgct tcggcctcca 21660gtcggcgtac tcggcgctct tccgcgccgg ccgccaggtg ctgctgtccg ggtgggacgt 21720cggccggttc ctggagctgg cccgccggga gcggcccagc gtggtggtgg cggtccccgc 21780ccaactgcac gacctggtca cccgggtgcg cgaggacgcg gacggccccg gcttccggcc 21840cggccggatc ctcaccgccg gagcggccct gcccccggcg ctcgtccgcg acgtgcggga 21900ggcgctggac accacgctgg tggtggtgtg gggcatgtcg gaggccggca acggcaccag 21960cagcctctcc gccgacgcgc cggaggtggt ctcccgcagc gtcggccggc ccacccgcga 22020cgccgagatg cgcgtcgtgg acgaggacgg cgccccgtgc ccgcccgggc agcccggcga 22080gctgtactac cgcagtccca gcatgttccg tggctacttc ggcgagccgg agctgacccg 22140gtcggtggtg tcggaggacg gctggctgcg caccggcgac ctcgcctcga tcggcgagga 22200cggcctggtc accttccacg gccggtcggc cgaactgatc aacgtcggcg gccgcaagtt 22260caacgcggtg gagatccagg cgctcctcgc ggacctgccg gacatcggcc cgctcgccgt 22320ggtcgccgcg ccggacccgc gtctgggcga gtacccggtc ctggtggtca ccgaacggcc 22380ggccgccgca ccggcggacg gcacggcacc gcgcccgcgc ggcacggtcg ggctcgacga 22440ggtgacggct catctccggg gcctgggcac cgccgagtac aagattccgc tggagctggt 22500ggcccttccc gagctgcccc gcactcccgc ggggaagatc aaccggcggg cgctggagca 22560gtacctcgcc gacgccgccg agcggaccgc cgttactccc gccgaggcgc cgcgccccgg 22620tctccggacg gcgctggagc tggtggtgac cgccgtcgcc gaggtgctgg ccgccgtgcc 22680cggcgaggac ggcgcgcggc ccgccgccgc cggtccgatc gggccggaca ccaccttccg 22740
cgcccacggt ctggactcgg tcgcctccgt acggctgcgc aacgcgctcg ccgaggccac 22800ggggctgacg ctgcccgccg gactcgcctt cgacttcccc accccggccg ccctggcccg 22860ggaactggcc gggctctcct ccccggccgc agaggagagc ccgggtgcgt ccgcccacga 22920ggacgagccc gtcgcgatcg tctccatggc ctgccgcctg cccggcggcg ccacctcccc 22980cgaggcgctg tgggagctgc tgcgcgacgg cgtcgacgcc gtgtccggct tccccgagga 23040ccggggctgg gacctggacg ccctcttcgg cgacgacccc gacgcaccgg gcacctcggt 23100ggcgcgcgag ggcgggttcc tgcgcgacgc ggcccacttc gacgccggct tcttcgggat 23160gtccgcgcgg gaggccctcg ccaccgaccc gcagcagcgg ctgctgctgg agacggcctg 23220ggaggccgtg gagcgcgccg gcatcgcccc gcgaaccctg cggggcagcc gtaccggtgt 23280gttcaccggc gcgatgtacc acgactacgc ggccggcgcg tccgacccgg ccggggagct 23340ggagagcctg ctgccggtgg gcaccgcggg cggcgcgctc tccgggcgga tcgcctacac 23400cctcgggctg agcggtccgg cgctcaccgt ggacaccgcc tgctcctcct cgctggtcgc 23460cctgcacctc gcctgccgct ccctgcgctc cggcgagtcg gacctggcgc tggccggcgg 23520cgtcgccgtg atggccaccc cggccgcgtt cgtgggcttc tcccggctgc ggggcctttc 23580ccccgacggc cgctgcaagt ccttcggcga gggagccgac ggagccgcct ggtcggaggg 23640ggccggcctg ctcatgctgg agcggctctc cgacgcccgc cgcaacggcc accctgtcct 23700cgcggtgatc cgcggctccg ccgtcaacca ggacggcgcc tccaacggac tgaccgcccc 23760ccacgggccg gcccagcgcc gggtcgtccg gcaggccctg gccgacgccg gtgtccgcgc 23820cgccgaggtg gacgtggtcg aggcgcacgg aaccggtacc gccctcggcg accccatcga 23880ggccgaggcg ctgctcgaca cctacggacg ggaccggccg gagggccgtc cgctgtggct 23940gggttcggtg aagtccaacc tgggacacac gcaggccgcc gccggcgccg ccgccgtgat 24000caagatggtg ctggccctgc ggcacgacct gctcccggcc accctgcacg ccgacacccc 24060cacctcccgg gtggactggt cccccggcac cgtgcagctg ctgacgcggg cccgcgactg 24120gccccgcgag gagggcaggc cgcgccgcgc gggcgtctcg tccttcggca tcagcggcac 24180caacgcccac ctcgtcctgg aggaggcacc cgtgcccgcc gccggcacgg agcggagcgc 24240ggacgcggga gccgccggcc tccgtgccgc cgtgccgtgg ctggtgtcgg ccaaggacgc 24300ggacgcgctg cgcggccagg cccggcgcct ggccgcacac gccgccgccc acccggaggt 24360gtccgcacgc gacctcgcct actccctgct caccacccgg gcgctgcacc cccgcaccgc 24420cctgctgacc ggcggcgacc gggacgcgct ggtcgcgtcc gccgacgcct tcgcccgtgg 24480cgaggcaccc gggagcatcg tccgtggccc gctcggtccc gcccccggga ccgccttcgt 24540cctcaccggg cagggcagcc agcggctcgg catggggcgc gggctcgccg ccgccttccc 24600ggtcttcgac gacgctctgc gcgaggtgtg cgcgctcctc gacccgctgc tggaacggcc 24660gctgaccgag gtcatgtggg ccgcgccgga cagtgacgag gcggggctcc tcggcggcac 24720gggctacgcc cagcccgcgc tcttcgcgtt cgaggtcgcc ctgtaccggc ttctggagtc 24780gtgggggatc gtccccgacc ggctggtggg gcactcggtc ggcgagatcg ccgccgccca 24840cgtggccggg gtgctctccc tgccggacgc ctgcgccctg gtcgccgcgc gggggcggct 24900gatgcaggcg ctgccgccgg gcggcgccat ggcggcggtg cgctgctccg aggcggagat 24960tctgccgctc ctggccggac gcaccgccgg ggccaccgtc gccgccgtca acggcccgcg 25020gtccgtggtg ctgtcgggca ccgaggaggc cgtggccgag gtcgtcacgg aggtctccgc 25080cgccgggcac aagacgcgtc ggctcatggt cagccacgcc ttccactcgc cgctgatgga 25140gccgatgctc gcggagttcc gggcgacggt cgccgggctg tcgttcgccg cgccccaggt 25200tccgctggtc tccggcgtca ccggacggcc gctcaccgcc gaggaggccc gcgacccgga 25260ccactgggta cgccatgcca gggacaccgt ccgcttcgcc gacgcgatca gccatcttgc 25320cggagaacac accgagatct acgtcgagtt gggcccggag gccgcactca ccccgatggt 25380
cgaggagtgc ctgggcgagc cggagagcgg cgacggtccc gccgtggagc cggtggtacg 25440gggcgacgtg gacgaggagc gggcggcact ggccgccgcc gtacggctgc acgcgctcgg 25500cctggacgtg cagtggcggg cggtgctgcc ggaggcccgc gcggtgccgc tgccgaccta 25560cgccttccag cacgaggcct actggctggc cacgtccggt tccgtggtcg cggggctgtc 25620gctgccgggc ggccgggcgg ccgacaccgt tccggacctg gccgggcggc tggcggggct 25680ctccggcggt gaggcggagg cgctcgtcac cgaactggtg cggaccgagc tggcggcggt 25740gaccggtggt gagatctccg ccgccggagc cggcacggcc ttcaccgagc tgggcgtcac 25800ctccgtcacg gcggtggagc tgcgcaaccg gctgaccgcc gtcaccggcg tgcggcttcc 25860cccgaccctg atcttcgacc accccacccc cacggccgtg gcccgcctca tcggcgagac 25920ggtccggggg agttcggtcc cgggccgccg ggacgccgtg tccctggtgg acgagttgga 25980ggcgctgctc gtctcgggcg ccgaggtcga ctcggacacg gcggcgcggc tgcggtccct 26040ggccggccgg tgggctccgt cggccaccgg aacggcggcc gacgcgaacg gtccgctgga 26100tctggacgac gcctccgacg aggagctgtt ccgcctcatg gacggcggcg ccccgtgact 26160ctctccgccc ggcggctcct cggcggcgtc cgccccgggc gagccgtcgg gcacccctga 26220ccgaccgcgt tccgggcggc cccgttgctc cggcgccccg gccggaacgc ggccccgcca 26280ggcggtccgc cggtcgccca ccttcacaga cccacgagcg gaagtacctc tcatgaacgg 26340gctccacctg ccccccgccc ccttgcccgc cccgggcgtg agcgccgccg gttccggcga 26400tctgatgatc gaggcggagg gcgtcagcaa ggcatacggc acggtccacg ccctggactc 26460ggtcagcctc gccgtccccc ggggcaccgt gctgggactc ctcggccaca acggcgccgg 26520gaaaaccacc ctggtcgaca tcctcaccac ggcgctgccg cccacctcgg ggcgggcgag 26580ggtcgccggg tacgacgtgg ccgacaagcc ggtcgagatc cgccggcgca tcgggctcac 26640cgggcagttc gcctcggtgg acgcgcagct cagcgggtac gacaacctcg tcctgatcgc 26700ccgtctgctg ggcgccgggc gccgggcggc ccgcgtgcgc gcgagggagc tgctggagct 26760gttccggctg acggaggtcg cggaccggcg tgcgagcagc tactcgggcg ggctgcggcg 26820gcgcctggac ctggccgtgt cgctggtcgg cgcgccggag gtgctcttcc tcgacgagcc 26880caccaccggc ctggacccct ccagccgcat caacctgtgg gagatcgtcg aggggctggt 26940cgagcagggc accacggtgc tgctgaccac gcagtacctg gaggaggccg accggctggc 27000ggaccggatc gcggtgctct ccgcgggccg ggtcgtcgcg gcgggcacgg cgcccgagct 27060gaaggccacc gtggggcgcc gcacggtgac gctcaccctg gagaccggcg aggacgtgag 27120cgcggcgcgc acggcgctgc gcggggcggg cttcgccccg gtcgacggcg aggcccgcac 27180cgtggtcgtc ccgatcgacg ccacacggga gatcgccgac atcatcagga gcctggacca 27240ggccggcgtc gaggcgagcg agctgaactt cggcgaaccc acgctcgacg acgtgtacct 27300gaccctcgcc gagcaggccg ccggccggac ccgggcatga ccgcgcggaa ccccgtcccc 27360cgcgggcccc gcctcccgcg ccccgacacc ccggcgggcg ctgccgccgg ccgacgacgc 27420ctttcacgga ggaccccttg accactcagc tcgccccgcc gctgcctccg gccgtgccac 27480ggcccgcggc ggtgcctgcc ttcggcggga gttcactgtt cacccagatc cgggtgctca 27540ccgggcggtc cctgcgggcc atggtgaccg acccgggcat cgtgctcttc ggcctgatcc 27600agccggtggt catcctcttc gtgctgaccc aggtcttcag caagatgggt atgccgcccc 27660acttcccgga cggcgtcagc tacctcgact acgtcctgcc cgccgtcctg gtggacaacg 27720ccgcccagtc ggcgatgcag tcgggcgtgg gcctggtgga ggaccagaag aacggcatcg 27780tggcccggct gcgctccctg cccgtccacc cgggcgcgct gctggccgcc cgcagcctgg 27840tcggcctggt gcgcagcgcc gtgcaggtcg cggtcatcat ggcgctggcg atgaccgtcc 27900tcggctactc ccccaacggc ggtgccgccg aactggccct ctccgcgggg ctgacgctct 27960tcatcagctt ctcgctgggc tgggcgttca tcgcggccgg cgcctggctg cggcgggccg 28020
agccgctgca gaacctcgcc ctgatcgtga tcttcccgct gatgttcgcc tccagcgcct 28080acgtcccggt cgcggacctg ccctcctggc tgggcgcggt cgcgagcgtc aaccccctga 28140cgtacgcgat cgacgcgacc cgggcactgg cgctggacgt gcccggcctg gaccacgcga 28200tcccggcggt ggtgatctgc gccgtgatcg cggtggtcgg catgatcgcc gccgtacgcg 28260gcttccgccg gcctctctga ctccccgcac gaactccccc cgtgcgtgcg gggacggggc 28320ccgtggacgc ctctcgcgtc cacgggcccc gttccttgcc cggaggaggc ggagacagcc 28380cctggcagga ggaaacaggc gacttcggac cgtccctggc tcccggacgg cgcccgtccg 28440gaccacagcg cgggcacccg aacgggcagc ggacgacggt cggtccggac cagggccgtg 28500gaacaccgga gcatacggag gtgcgcaccg gtatgtcggc cccgagtagc gggcaaaaac 28560gggcacagct ccgcacggaa acggaccgag agactcccat atctgtctca cttctgtcag 28620gagcgctcgt ccgcagcccc tgagacggtc tcggcacccg gctcaacggc gtccggaacc 28680cactgcggcg accccgaaac cgggcggtgg tgatccccac cgcgaggctg gagccgctcg 28740gtcactccct gcccgcaacg ggcacggctc acgtgccgag gggcgcacgg aacgcacacg 28800gcccacgccc gaaactctct gaccagcggg gcaagctccg catccccaca gcaaagcagt 28860ccgcgtcggg ccgggaacgg gcagaatcgg gcctcttcgc ttccggacgg cgggcaggag 28920aacggccctg gggccggcac cgtggtgccg gccccagggc cgttcgttcg cggcctgtgc 28980ggtcaggaga gtccgagttc gttgtcgagc atgtcgaaca tgtcctcgtc ggaggcggag 29040gcgaagtcga aggactcctc cgccttctcc ccgcccttga gggcctcggc ccgcatctcc 29100tgccacttgc cgcggagaac ctccagccgg cccgccacct ggtcgaagag ctcggcgccg 29160acctcggcgc ccgcgaaagc cctctccagc ttgtccagtt ccgccaggac caccgccgac 29220ccgtcggccg gtgccggggc cacccgcgtg tacaggtggg cgacgagttc gccgggcgtc 29280gggtagtcga acagcagcgt ggccgggagc cgcaccccgg tgagcgcgcc gagccggttc 29340cgcagctcga ccgaggtcag cgaatcgaag cccaggtcct ggaaggcacg ggcgggttcg 29400atgtcggcct ggcccgcgtg gcccagtacg gtcgcgatct ggccgcgtac caggtccagc 29460aggacctcct cgccctcggt ccgggtgagg ccggtgaggc gccgggtgag gtcggcggcg 29520gccgccgctc ccccgtcgga ggcggcacgg cgggcctggg tccggatcag tgaccggaac 29580agcggcggca cctcgccgag ggagcgcagg gcccccaggt cgagccggac cgggaggacg 29640gccggggcct gctcggcggc gccggtgacc gcgtcgaaca gggccaggcc ctgctcgggg 29700tcgatcgggg gcatgcccgc ccgcgccatc cgggcggtgt ccgcctcggt cagctcaccg 29760gtcatgccgc tcccccgggt ccacgggccc caggccatcg acaccgcggg cagtccacgg 29820tcccggcggt ggcgggccag tgcgtcgagg aaggcgttgc ccgccgcgta gttggcctgt 29880cccgcgctgc cgagcgtgcc ggcgacggag gagaagagca cgaaggcgtc gagcgtaagc 29940ccctcggtcg cctcgtggag gtgccaggcg gcgtccgcct tgggccgcag cacggcggcg 30000aggcgttccg gggtgaggga gtcgagaacc ccgtcgtcca ggacaccggc cgcgtggatc 30060accgcccgca gcggacggtc ggcggggatc tccgccagca gcgcgtcgac ggccgtacgg 30120tcgccgacgt cgcaggcggc gacggtgacg tgggcgccgg actcctccag cgcggcggcc 30180agttcaccgg caccctcggc ggccggacca cgacggctga ccaggagcag gctccggacc 30240ccgtgctcgg cgaccaggtg ccgggcgacg gtggcgccga ggccgccggt gccgcccgtg 30300atcagcacgg tgccctccgg gtcccagccg gccgcgtcgc tctcggcggg cgtcaccgcg 30360cgcaccaggc gaggtgccag caggcggtcc tcgcgcaggg cgagctgcgg ttcgcccgag 30420gccagcgccg cccgcagggc ggacatcccg agcggagagg tggccgaggg ctccaggtcg 30480aggaggatga accggcccgg gttctccgcc tgcgcggcgc gcaccagccc ccagaccgcc 30540gcggcggccg ggtcggcgcc gtcggaggca ccccgggtga cgacgaccag gcgggaggcg 30600gcgaaccgct cctgggccga ccagtcgcgg agggtctcca ggacccgggc ggtggcggcg 30660
tgggcggcgg cggccacgtc cccggtgccg gacccggcct ccggggcggt ggcgaccggc 30720agcagcacca cggacggcac gtcctccccg ggcccggtgc cggtgacgag gtccgccaga 30780ccggtcacgc cctccgtccc ggcttcggcg aggaggtcgc cgccgccgag gagcgcggtg 30840tccgggccga gcacggcgac cgggccgggc gtctcggcgt cccgcgtggc ggggtccccg 30900gcagccgccc agtccagctg gaacagggag tcgcggcccg cgccgcccgc gccgccgaac 30960tgcccggcgg agagcgggcg ggtcaccagg gacccgaccg aggcgaccgg ggtgccggcg 31020gtgtcggtga cggcgatcgc gagggtgccg tcgtcggtac gggagagccg cagccgtacc 31080gaggaggcgc ccacggcgtg cacggagacc ccgttccagg agaacgggac gccgccggtg 31140ccttcggact ccccgccgtc gccggccagg gcggtggcgt ggagtccggc gtcgaagagc 31200gccgggtgca gggtgaacgc gtcgccgtcg gtgccgtcgg gcagcgacgc ctcggcgaag 31260acctcgccgt cgcggctcca ggcggcacgc agcccctgga agacgggccc gtaggcgaac 31320ccggcgtcct cgaaacgctc gtacagcccg gtggtgtcga gcggctcggc cttctccggc 31380ggccacacgg tggcgtcgaa gtcggtggcg ggggcctgtg ccggtccggc ggccagggtg 31440ccctcggcgt gcgaggtcca cggggcgccg gagtcgtcgg cgggccggga gtggatggtg 31500atcccgcgcc ggccctcctc gtcgggggcg ccgacgccga cctgcacctg gacggtgccc 31560tgctcgggca ggaccagcgg ggccgccagg gtcagttcct ccaccacgtc gcagccgagc 31620acgccgcccg cgtggactgc gagttccagc agggcggtgc cgggcaccag gacggcgccc 31680atgacacggt ggtcggagat ccagccgtgg gtgccctggg aaagacgtcc ggtgaacagg 31740tggccgtccg tgccggccag ttcgaccgcg ccgccgagca gcgggtggcc ggcgggcgcg 31800agcccggcgg cctccgcccc ggcgcccagg ggcatcatcc cggcgggcca gaaccggcgc 31860cgctggaagg cgtacgtggg cagctccacc cggcgggcgc cgcggccggc gaagaacgcg 31920ggccagtcga cgcggacacc gtggccgtgc agccgggcga ccgcctcgac cagggcctgc 31980tcgccgggga cggtgcggcg ctgggcggcg accgtcacgg cggactcggg cagggactcg 32040cgggccatgg cgcaggcgac accgtcgggg cccagttcca ggtagcgggt ggcaccggcc 32100tcgtgcaggg cccggacgcc gtcggcgaag cggacggcct cccggacgtg gcgcacccag 32160tactcggcgg agcacagttc ctccgcgcgg gccgtggtcc cggtgaggtt ggagacgacg 32220ggtatcacgg gtgcctgcgg ggagaggccg gcgacgacgg cccggaactc ggcgagcatc 32280ggttccatca gcggcgagtg gaaggcgtgc gagacgcgca gcgcggtcgt ccggcgaccg 32340cgctcccgca gggcctcggt caccttctcc accccttcgg cggtgcccga caccacgacg 32400gacgtgggtc cgttgacggc ggcgaccgag accccgtcgg tgaggtgcgc gagcacctcg 32460tcctcggtgg cctggacggc ggccatcgcg ccgccctccg gcagtgcctg catcaggcgg 32520ccccgcgcgg cgaccagggc gcaggcgtcg gccagcgaga agaccccggc gacgtgcgcg 32580gcggcgatct cgccgatcga gtggcccgcc accaggtcgg gggtgacgcc ccacgaggcg 32640acgagccggt ggaggcccac tccgagggcg aagagcgcgg cctgcgcgta ggcggtggcg 32700ttcagggcgt cctcgtcgtc gccccagacc acctcccgca gcgggcggtc caggtgcgcg 32760tcgagtccgg cgcagaccgc gtcgaacgcc tcggcgaaca ccgggaagcg ctcgtagagg 32820tcgcggccca tgccgagccg ctgggagccc tgccccggga agaggagggc gagcagcccc 32880tcaccggtcg tcgtggccgg ttcctcgccc cgggccacgg agccgagcgc ggccagcaga 32940ccgtcccggc cggcgcccgt gacggcgacc cggtgctcga acgcggtacg ggcggtggcc 33000agagagtagg cgacgtccag cgggcgcgcc tcggttccgc cggcggccgg gtggtccccc 33060gtcaggtggt ggaggagccg ctcggcctgg gcgcgcaggg cgtcccggtc gcgggcggac 33120agcagccacg ggacgacagg ggcgacgccg tcgtcgtccg cgccggtcgc gggctcgtcg 33180gacggcacgg ccgcgggcgc ctgctcgacg atgacgtggg cgttggtgcc gctgatcccg 33240aaggaggaga tcccggcgcg gcgcgggcgg ccggagccgg tccactgccg ggcctcggtc 33300
agcagctcca ccgacccggt ggtccagtcg acctggtcgc tgggggcgtc gacgttcagc 33360gtggcgggca gcagcccgtt gcgcagcgcc agcaccatct tgatgacgcc ggcgactccc 33420gccgcggcct gggcgtgccc gatgttcgac ttgaccgagc cgagcagcag cggccggtcc 33480tcggggcggt cctggccgta ggtggcgagg agcgcctggg cctcgatggg gtcgcccagg 33540gtggtgccgg tgccgtgcgc ctcgacggcg tcgacctggt cggcggcgag ccgggcgttg 33600gccagggcct gccggatgac gcggcgctgg gacgggccgt tgggcgcggt gaggccgttg 33660ctcgcgccgt cctggttgac ggcggagccg cggacgacgg cgagtacctc gtggccgttg 33720cgcaccgcgt cggagagccg ctcgacgacg aggaagcccg cgccctcgga gaagccggtg 33780ccgtcggccg ccgacgcgaa ggacttgcag cggccgtcgg gggcgaggcc gccctggcgg 33840ccgaactcgg cgaacacggc cggtgtcgac agcacggtga caccaccggt gaccgccagg 33900gagcactcgc cctgccggag cgcctgcacg gcgaggtgca gggcgaccag cgaggaggag 33960caggccgtgt cgacggagac ggcggggccc tccagtccga gcttgtaggc gacgcggccg 34020gtgaccacgc tgccgttgcc gtcgctgccc gggtagtcgt ggtacatcac gccggtgaag 34080acgccggtcc ggctgccctt gagcgaggcc gggtcgatcc cggcccgctc cagggcctcc 34140caggacatct ccagtgccag gcgctgctgc gggtccatgc cgagcgcctc gcgcggcgag 34200atgccgaaga aggcggggtc gaagtcggcc gccccgtcga ggaagccgcc ttcccgggcg 34260tacaggccgt ccgggtggtc gccgtcgggg tcgcggagga tcgacaggtc ccagccgcgg 34320tcctggggga aggggacgat cgcgtcgtcg ccggaggtga ccaggcgcca gaggtcctcg 34380ggcgagctga cgccgcccgg gtagcggcag gccatgccga cgatggcgat gggttcgtgc 34440tgggccgagg tgagcctgcg gttgtcgcgg cgcagccggg cggtctcggc acgggcggca 34500cggagcgcgt cggcgcccgg ttcggtggcc gtgccggtcc cggcggtctc ggccgcgccg 34560tcgctctcgt cgtacggtcc ggaggcgtcg tcctcgtcgt cgccgtcgaa ggcggcgagc 34620agggcgtccc ggtcggtgtc ggacatctcg tcggagtccg cggcgagttc gagcagcgtc 34680tccgccagcc ccgcgtcacg caggcggcgg gccgggatgg cggagagggc gcggcgcacc 34740ccgtcgtcgt cgccggtggc accgcccgcg ccggaggcgg ggtgggcggc ggcgagttcc 34800tcctcgacga cctcggtgac ggcgagggcg ttggggtggt cgaagacgag ggtggcgggc 34860agcctcagtc cggtcagcgt gttgagctgg ttgcgcagct cggtcgcggc cagggagtcg 34920aagcccagtt cctggaatgc ccggtcagcg ccgatcgcct ccgccgaacc gtgccccagc 34980accgccgcga cctgcgaccg gacgagatgc agcaccgcgc ggtgccgttc gggcgcgggc 35040agttcggcga gcctgcggga cggggagtcg tccgggcccg gcgcggcagc cgaacccgag 35100gccgcggtgg cgcggcgggc gggagccagc gcgcgcagga gggcggggat ctcgtcggtg 35160cgggtgcgca gcgcggcggt gtcgacgcgc agcggcacga cggtcgcctc gcttcggtcc 35220aggccggccg cgaacagctc cagccccgcc tcgtgcgaca gcaacggcag cccctgcgac 35280gccatccgac gccggtccac ctccgacaga tactccccca gacccgcacc cacgtcccag 35340aacccgaacg ccatggacgt ggccaccaga ccctcggccc gacgccgcga cgccaacgcg 35400tccaggaaca cattggccgc cgcgtaattg ccctgccccg ccgtcagcac cagaccaccc 35460gccgacgaca ccagcacgaa cgccgccagc tccacacccg ccgtcgcctc gtgcaaccac 35520cacgccgcat cagccttcgc acccagcacc gcatcgaacc ggtcgggagt gagggagccc 35580aggagcccgt tgtcaccgac tccggccgcg tggaccacgg ccagcagccc gcgcccggag 35640acgagagagc cgacgaggtc cacgaccgat tcgcggtcgg acaggtcgca cgcgacgacc 35700tccaccgccg caccggcctc acgcagttcc ccggccagct ccgccgcacc cggggcgtcc 35760ccgccccgcc gcgacaccag caccagatcc cgcaccccac gctcagccac cagataccgg 35820gccatcaccg cacccagacc acccgtacca cccgtcacca gcaccgcacc ctcaccatcc 35880aacgacggca cggacagcgg cagttcggca gcgggtgcgt cggcggtcag ccgcgtcagc 35940
ctcggtacga ggaccacgcc gtcccggacg gcgacctcgg cctcgcccga gaggacagcc 36000gcgaccgcac agtccaccgc cgcatcatcc gcgtccacgg cgacatcggc cagggcgaac 36060cggcccggat tctccgcgag cgccgcccgc accagacccc acacaggggc ctgcacgacg 36120tcgccctcgg ccggggcgcc gccggaaccc acgggcatag cgccccgggt caccacgacg 36180agccgggagg cggcgaaccg ctcctcggtc agccagcgct gtgccacggt cagcacaccg 36240tccagcgcgg agcgcaccga gccgggcacg tcggccccgg ccggagcgga ggcgtccagc 36300acgaccacgg cgggcacctc ggtaccggcc tcctcgaccc gggaccaggt ggcccaggcc 36360gtgtcggtgt cgtcgagccg ggtcacgggg acccactcgg gggcgtagag cgcgcccgcg 36420tcacggggcg ccgcaccgag ctggccggcg gagacggtgc ggctcaccat ggagccgacc 36480gtcaggaccg gggctccggt gacgtcggtg acgcggatgt ccataccgcc gtcggcggcg 36540gtggcgatcc ggacgcggac ggcggcggcg ccgaccgcgt gcagggcgac ccggttccac 36600gcgaacggga tgacggtccc gccgctcccg ccctcggcgt tgtcgttgct gtcgttgctg 36660tcgttgctgt cgttgaggat cgcgacgtgc atggcggcgt cgagcagcgc cgggtggagg 36720ccgaagcggc ccgcctccga ggcagcctcc tccgggagcg ccacctcggc gaagagttcc 36780ccgccccggc gccaggcggc gcgcagcccc tggaagaccg gcccgtagcc gtagccacgc 36840tcgcggaaga tctggtacgc gctgtccacg ggcacgctcg cggccccggt cggcggccac 36900tgctccagtg cggcctcggc ccctgccacg agggcgttgt cggtgaggaa gccggtggcg 36960tgggtggccc actcctgccc ggtgccctcg gggcgtgagt agacggcgac cgtgcggcgt 37020tcggcggtgc gcggtccgac gaccacgcgg acctgggcgc cgccgtgctc gggaagtacc 37080aggggtgtcg ccagggtcag ttcgtcgagt acggcgcagt cgacggactc tccggcggcg 37140agggccagtt ccacgaggac ggcgccgggc agcatgaccc ggccgaggac cacgtggtcg 37200gcaagccacg gctgcgcctc caccgacagc aggcccgtca gcaccgcgcc tccggagtcc 37260ggcagcgaca ccaccgcgcc cagcaacgga tggtcggccg cgtcgagtcc ggctccggcc 37320gcggccgggg ctccgtcggt ggcggtcagc cagtaccgct ggcgctggaa ggcgtaggtg 37380ggcaggtcga cggggccggc gccggagccg gcgaagaagc cggcccagtc gacggccgtg 37440cccgcggtgt gcagctgcgc gagcgcggtg agcagggcgt cgggctcctc ccgtcccttg 37500cgggccgacg gcaccagcag gactgcggtg tcctcgtcgc cgtcgaggca ggtgcgggcc 37560atgccggtga gtacggcgtc ggggccgatc tcgacacagc ggtccacgcc cgcggcgcgc 37620agtgcccgga cgccgtcgcc gaagcggacg gcctcgcgga cgtgggtgac ccagtagtca 37680ggtgtggcca gttcaccgga ctccgcgatc cggcccgaga cgttcgagac gaccggcagc 37740gacggctccg cgtacgacac cccggacaga acctctcgga agtcagcgag catcggttcc 37800atcagcggcg agtggaaagc gtgcgacacc cgcagctggc tgacccgccg tccctgctca 37860cggaacacct cagccaccgc ctcaacggct tccccggcgc ccgagacgac caccgacgac 37920ggaccgttga ccgcagcgac cgacacacct tcgacggact ccagatgcgg cagcacctcc 37980gtctcggacg cctccacggc caccatcgcg ccgccctccg gcaacgcctg catcagccgc 38040ccacgagcag ccaccagagc acaggcgtcc gccagcgaga acacacccgc cacatgcgcg 38100gccgccacct caccgatcga atggccggcc accaggtcag gcgtgacgcc ccatgaggcg 38160agaagccgga agagggcgac ttcgtaggcg aacagggcgg actgggcgta cgccgtgccg 38220tcgaggtccg gcgcgttctc gccccaggcc acctcgcgca gcgggcggtc caggtgctcg 38280tcgagcccgg cgcagaccgc gtcgaacgcc tccgcgaaga cggggaacgc ctcgtggagg 38340ccgcgcccca tctccaggcg ctgtgcgccc tgcccggaga agaggaaggc cgtggtaccg 38400acggagcggg cggttccggt gatgaccccg ggggcgtgct cgccggccgc gagggcgcgc 38460aggccggtca ggaggtcgtc ccggccggtg ccgaggacca ccgcgcgggc ctccagcggg 38520gtgcgggagg tggccagcgc gtgggcgacg tccagcggac gggtgccggg ccggtccgcc 38580
aggtggtcca gaaggcgggt ggcctgggcg cgcagggcgt ccggggtggc cgccgagacc 38640gtccacggga ggacggcggg ggcgcggccc tcctcggccg tgctcggcgc gtcggctgcg 38700gcgtcggggc cctgttccag gacgacgtgc gcgttggtgc cgctgaggcc gaacgccgag 38760acgctcgccc ggcgcgggcg gccggtgacc ggccacggcc ggtcctcggt gacgagttcg 38820gcgcggccct cggtccagtc gacgtgcggg gacggccggt cgacgtgaag ggtgcgcggc 38880acggtgccgt gctccatcgc catgaccatc ttgatgacgc cggcgactcc cgccgcggcc 38940tgggcgtgcc cgatgttgga cttgaccgag ccgagcagca gcggccggtc ctcggggcgg 39000tcctggccgt aggtggcgag gagcgcctgg gcctcgatgg ggtcgcccag ggtggtgccg 39060gtgccgtgcg cctcgaccac gtcgacttcg gaggcgggga ccccggcgcc gtgcagagcc 39120tggcggatca cgcgctgctg ggaggggccg ttgggggcgg tgaagccgtt ggaggcgccg 39180tcctggttga cggccgtgcc cttgacgacg gccagcacgc ggtggccgtt gcgcacggcg 39240tcggagagcc gctccaccag gaggacgccg acgccctcgg accagcccac gccgtccgcc 39300gcgcccgcga acgccttgca gcggccgtcc accgacaggc cacgctggcg gctgaactcg 39360acgaacattc cgggcgtcga catcacggtg gcgccgccgg ccacggccag cgagcactcg 39420cccgagcgca gggcctgtac cgcgaggtgc agcgcgacca gggacgagga gcaggccgtg 39480tccacggtga cggctgggcc ctcccagccg aaggtgtacg acaggcggcc ggagaccacg 39540ctgccgccgg cggtgccgga gggcgagacg ttcagcgcgt agtcgtggta catgacgccg 39600gcgaagacgc cggtgcggct gccccgcagc gtcgccgggt cgatcccggc gcgttccagc 39660gcctcccagg aggtctccag gagcaggcgc tgctgcgggt ccatgtacag ggcctcgcgg 39720ggcgagatgc cgaagaagcc ggggtcgaac tgggcggcgt cgtgcaggaa gctgccgcgc 39780ctggtgtagc tcttgccctc cttgccgggc tcggggtcgt agaggccctc gatgtcccag 39840ccccggtcac cggggaggtc ggagacggtg tcgacgccgt cggtgaccag gcgccacagc 39900tcctcggggg tggtgacgcc gccggggtag cggcaggcca tgccgacgat ggcgatgggc 39960tcgtcgtcgg ccggtgctgc cgtgcccgta cggacggggg tctccgcccg cgcggactcc 40020ccggccagtc gcgccgagag gtgatcggtg acggcgaggg cgttggggtg gtcgaagacg 40080agggtggcgg gcagcctcag tccggtcagc gtgttgagct ggttgcgcag ttcggtcgcg 40140gccagcgagt cgaagcccag ttcctggaag gcccggtcag cgccgatcgc ctccgccgaa 40200ccgtgcccca gcaccgccgc cacctgcgac cggacgaggt ggagcagggt gcggtgccgc 40260tccgcctccg gaagcgaggc cagccggtgg gccggcgagc cctcgacggt ggccgggggg 40320accacggccg cggagcgccg cacgggagcg agggacttga gcagggcggg gatctcgtcg 40380gtgcgggtgc gcagcgcggc ggtgtcgacg cgcagcggca cgaccgtcgc ctcgccccgg 40440tccaggccgg ccgcgaacag ctccagcccc gcctcgtgcg acagcaacgg cagcccctgc 40500gacgccatcc gacgccggtc cacctccgac agatactccc ccagccccgc acccacgtcc 40560cagaacccga acgccatgga cgtggccacc agaccctcgg cccgacgccg cgacgccaac 40620gcgtccagga acacattggc cgccgcgtaa ttcccctggc ccgccgtcag caccagacca 40680cccgccgacg acaccagcac gaacgcagcc agctccacac ccgccgtcgc ctcgtgcaac 40740caccacgccg catcagcctt cgcacccagc accgcatcga accggtcgga cgacagcgtg 40800cccaccaggc cgccgccgcc gaccccggcc gcgtgcacga cggcgcgcag cccacgcccg 40860gcgacgaggg actcgaccag gccgacgacc gattcgcggt cggacaggtc gcacgcgacg 40920acctccaccg ccgcgccggc ctcacgcagt tccccggcca gctccgccgc gcccggggcg 40980tccccgcccc gccgcgacac cagcaccaga tcccgcacac cacgctcagc caccagatac 41040cgcgccatca ccgcacccag accacccgta ccacccgtca ccagcaccgc accctcacca 41100tccagcgacg gcacggacag gggcagttca ggctcggcga gacgactgag cctcggcacc 41160agcacaacac cgtcacggac ggcgacctcg gcctcgcccg agagggcagc cgcgaccgca 41220
cggtccaccg ccgcgtcatc cgcgtccacg gagacatcgg ccagggcgaa ccggcccggg 41280ttctccgcga gtgccgcccg caccagaccc cacacggggg cctggacgac gtcggcggat 41340gaaccggccg gggcggcgcc acgggtgacg acgaccagac gcgaggaggt gaagcgctcg 41400tcgcggagcc actgctggat cgcgggaagg acctggtcca ggacggcccg gacggcggca 41460gggacgtccg gaccggcggg ctcgcgcacc tccaggacga ccgcgcccgg cgccgtggtg 41520tccgcctcca gggcttcggc ccaggagacg tgggcggggg ccgtggaggt gtccacggtg 41580accggcgtcc actcgatgcc gtagagggcg cccgcgccgg cggaggcgct gccgagctgt 41640tcggcggaga ccgggcggct cgccagggag cccacggtca ggaccggcgc gccggtggag 41700tcggcgaggg tcagggagac ggtgtgggcg tcgacgcggc tcacgcgtac gcggacggcg 41760gaggcgccga cggcgtggag agtcacgtcg ttccaggcga acgggacgac cgcctggccc 41820tcctcctcgt tcaggatgcc ggcgtgcatg gcggcgtcga gtacggccgg gtgcaggccg 41880aaccggcccg cctcacctgc cgcgtcctcg gggagcgcca cttccgcgaa catcgcgtcg 41940tcgcgccgcc aggcggcgcg caggccctgg aagaccggcc cgtagccgta tccccggtgg 42000cggaactcct cgtaggcgtg ctcgaccggt accgggtcgg cgccgggcgg gggccactcg 42060gtcagcgctt cgccggagcc tgccggggcg tcggtgagga agccgtgcgc gtgggccgtc 42120cacggcgcgt cctccgcgcc ctcgggacgg gagtggacgg ccacggtgcg gcgtccgtcg 42180gtcttcggcc ccgcgacgac gcggagctgg aggccggtgt cctcgggcag caccagcggc 42240gcggcgaggg tcagctcctc gagagcggtg gagccggccg cgtccccggc cgcgagggcc 42300agttccacca ggcccgtacc gggcagcagg acccggccga ggaccacgtg gtcggcgagc 42360cacggctgcg cctccaccga cagccggccc gtcagcacca cgccacccga gtccggcagt 42420gacaccaccg cgcctagcaa cgggtggtcg gccgcgtcga cccccgccga cctggggtcg 42480gcggcggcgc ggaccgtggt ctccggccag aagcgccgct tctggaaggc gtaggtcggc 42540aggtccacgg tgcgggccgc ggtaccggcg aagaagccgg tccacttcac ctcggcgccc 42600accgtgtgga gacgggccag cgcggcgagc gccgtcgacg tctcctcacg gtccttgcgc 42660aggagcggga cgaggacggc gtcctcaccg gcgccctcgc gcgccatgcc gctgagcacc 42720ccgtccggac ccagctccac gaaccgggtg acgccctcct ccgcgagggc ccggacaccg 42780tcgtcgaagc ggacggcctc gcggacgtgg gtgacccagt agtcaggcgt ggtcagttca 42840ccgggttccg cgatccggcc cgagacgttc gagacgaccg gcagcgaagg ctccgcgtac 42900gacaggccgg acagaacctc tcggaagtca ccgagcatcg gctccatcag cggcgagtgg 42960aaagcgtgcg acacccgcag cctgctgacc cggcgtccct gctcacggaa gacctcggcg 43020accgcctcga cggcgtcctc ggcgcccgag acgaccacgg aggaagaccc gttgacggcg 43080gcgacggaca ccccctcgac ggattccagt cgcggcagca cctccgcctc cgacgcctcc 43140agggcgacca tggcgccccc ctcgggcagc gcctgcatca gacggccacg ggcggcgacg 43200agcgcggcgg cgtcggcgag ggtgaacacc ccggccacgt gcgcagcggc gacctcaccg 43260atggagtgac cggccacgaa ctccggccgc accccccacg actcgaccag ccggaacaac 43320gccacctcga cggcgaagag accggcctgc gcgtacgccg tcccgtccag aaccgacgtg 43380tcgtcgcccc agaccacctc acgcagcggg cgctccaggt gctcgtccaa cccggcgcag 43440accgcgtcga acgcctccgc gaacaccggg aaacgctcgt acagcccacg ccccattccc 43500agacgctgcg acccctgtcc cgagaacagg aacgcggtac ggcctccggt ggccacgcct 43560tccacgacgc ccgcgtcggg cagacccgag gccagggctt ccagggccgc ttcacggtcg 43620gtgcccagga ccaccgcccg gtggtcgaag gtggcgcggc cggagaccag cgagaaggcc 43680acgtccggcg cgctcaccgg ttcggcggcc acgtgcacgc gcagccgctc ggcctgggcg 43740cgcagcgccg gggccgaccg tgcggagagc acccacggca cgtcgacggc gggcgccacc 43800gtgggcgcgg gctcctgcgc ctcctccggc tgttcgagga tgacgtgggc gttggtgccg 43860
ctgatcccga acgaggacac accggcccgg cgcagccgct cctcccccgg ccacacccgc 43920tcaccggtca gcaactccac agcaccggcc gaccagtcga cgtgcgacga cggagcatcc 43980acatgcagcg tgcgcggtag cctgccgtga cgcatcgcca tcaccatctt gatcacaccc 44040gccacaccgg cggcagcctg cgtgtgcccg atgttggact tgacggaacc gagcagcaac 44100ggccgctcct cgtcccggcc ctgcccgtac gtcgccagga gcgcctgtgc ctcgatcggg 44160tcacccaacg tcgtccccgt accgtgcgcc tccacgacat ccacatcacc ggcggacaga 44220ccaccgctcg ccagagcctg acggatgacc cgctgctggg aaggaccgtt gggcgccgtc 44280agaccgttcg acgcaccgtc ctggttcacc gccgaaccac gcaccaccgc cagcacacga 44340tgaccgttgc gccgggcatc cgacagacgc tccagcacca ccatgcccac gccctcggac 44400cagcccgcgc cgtcggcggc gtcggagaac gccttgcagc ggctgtccgg ggagagggcg 44450ccctggcggg agaactccac gaaggcggtg ggggtggcca tgacggtgac gccgccggcc 44520acggccagcg agcactcacc ggagcgcagc gcctgcgccg cccagtgcat ggcgacgagc 44580gacgaggagc aggcggtgtc gaccgtgacc gcgggtcctt cgaagccgaa ggtgtaggag 44640acacggcccg aggcgatgct gcccgcgctg ccgttgccgc gatagccctc gtactggtcg 44700tcggtgagga tgctgccgta gtcgttgtac atgacaccgg cgaagacacc ggtctggctg 44760ccgcgcaggg agaccgggtc gataccggac cgctcgatcg cttcccacgt cgtctccagc 44820aggagacgct gctgcgcgtc cgtggagacg gcctcacgcg ggctcatccc gaagaactcc 44880gggtcgaact cgggcgcgtc gtgcaggaag ccaccggacc gggtgtacgt ggtgccgggc 44940acgccccggt cctcgctgta gagggattcc acgtcccagc cacggtcgga ggggaagtcc 45000ccgaccgcgt cgacgccgtc ggagacgacg ctccacaggt cctcggggga cgtgacgccg 45060cccgggtagc ggcaggccat gccgacgatg acgaccgggt cgtcggccac ggagggcagc 45120gccgcgacgg ggaccgccac ggccggctcg gtgccgagga cgccgtcgag gaggtgaccg 45180gcgaggaggt cggcggtcgg gtagtcgaag acgaccgtcg cgggcaggcg caggccggtg 45240accttgccga gccggttgcg cagttccaca gccgtcaggg aatcgaaacc gaggtcctgg 45300aaggcgcggt gcgggttgac ggtctgggca ccggaatggc cgaggacgac ggcgatctgg 45360ccacggacca ggtcgagcag cacctcgcgg cgctcggcct ccgcgaggcc ggcgaggcgc 45420gcggccaggc cggtggcggc ggcaccggct gcggcggcgg tgcggcggac ggaggtgcgg 45480atcagcccgc tgagcagcgg cggcacctca ccctgggcgc gcagcgccgg gaggtcgagg 45540cggacgggaa gcacgaccgg ctcacccgaa gccagggccg cgtcgaacag ggcggtcccc 45600tgctcggcct cgaccggcgg cagaccggac cgggcgatac gccgccggtc ggtctccgac 45660aactcccgcg tcatccccgc gtcctgcgcc cacggacccc agacaagaga gacaccaggg 45720agccccaggg cccgccggta ctccaccaac gcgtccagga acacgttccc cgccgcgtac 45780gcgccctgcc ccgcactccc gaacgtcccc gccaccgacg agaacaccac gaacgcctca 45840aggcccaggt cccgcgtcgc ctcgtgcagg ttccacgccg cgtccacctt cggacgcaac 45900accgccgcga cgcgctccgc cgtcagcgat gcgagcgtgg cgtcgtcgat gacgccagcc 45960gcgtgtacca ccgcgcgcac gccgtgccgg gtgacgagcc cggcgacggc atcggcgtcg 46020gcggcgtcgc atgcctcgac ggccacgtgt gcgccggcct ccgtcagttc ggtgacgagt 46080tccccggcgc cctcggcgtt ggcaccgctc cggctgacca gcagcaggtc ccgcactccg 46140tgcgtcatga cgaggtggcg ggccagtacc cggcccaggc cgccggtacc gccggtgatc 46200agcaccggac cggtgagggc gtcccagcgc aggccctcgg ttgcttccgg tgtgacgcgg 46260gccaggcgcg gcacccgcac ctcgccgtcc cgcaccaccg actccgcctc gccggaggcg 46320agcgcggagc ggagggacgc ggtgtccgcg tcggccggaa gctccaacag gccgaatcgg 46380cccgggtgtt cggagatcgc ggagcgcacc agcccccggg cggcggcgtc ggcgagtccg 46440gtggtgcggt cggtggaggc cgtgacgaag accaggcggg atccggcgaa ccggtcctcg 46500
gccagccacg accgcaccag ggcgagagcc cgggcggcgg cggtgtgcgc ggcctgggcc 46560ggatcggtcc cgtcggcgtc gtcggaggcg gccaccagca ccgtggaggg catctcctcg 46620gcggccgcca gctcctccaa ggtggcgtac gtgacgaagt cgtcggcggc cggcacggcg 46680ctccgggcct cgccgaggac ggccaccgca ccggtgtcgg caggaccgtc cagcggccgg 46740gccgggaccc actccacctg gtagagggag tcccgcgccg gggtggtgag ctgtcccgtg 46800gcgagcgggc gcaccgtcag ggagtggaca gtggcgaccg gggaaccagc ggggtcggcg 46860agggcgaggg ccatggtgcc gtcggcgtcc cgggtgagac ggacgcggag ggccgtggcg 46920ccggaggcat ggagggaaac gccgccccag gagaacggga cacctccacg gccgtcgtcg 46980gcgagggcga aggcgtgcag cgcggcgtcg aagagcgcgg gatgcaggcc gaaggcgtcg 47040ccgtcggtcc ccccgtccag ggcgacctcg gcgtacaggg cgtcgccgtg ccgccaggcg 47100gcccgcagcc cccggaaggc ggggccgtag gcgaagccgg cctcggccag gcgctcgtag 47160cagtcggtca cgtcgacctg ggccgcgtcg cgcggcggcc agacggtggc gtcgaagggg 47220tcgggggcga gggtctcggg ggcgagggtt ccggtggcgt ggacggtcca cggagcaccg 47280tcgtcgtcct cggtgcgcgc gtgtacgccg agggcacggc ggccgtcctc gtcgggagcg 47340gcgacccgta tctggatctg gagggcgccg cgttcgggca ggaccagcgg ggcggagagc 47400gtcagttcct cgaccgatcc gcagcccgcc tcgtcggcgg cacggacggc catctccagc 47460agggccgtgc ccggcaccag cacggtgccc tggacgacgt ggtccgcgag ccacgggtgc 47520gaccgcgtcg acaggcgtcc ggtgaggacc accccgtccg cgccgtcggc gccggcgagt 47580tcgacggccg cgccgagcag cgggtgaccg gcggagccga gacccgcgaa gcgcacgtca 47640ccggcgcgcg ggagcgtggc ctcgggccag tagcgctgct tctggaaggc gtaggtcggc 47700aggtcgacgg ggcgggcgcc cacgaggaag ccggaccagt cgacgtccac gccgacggtg 47760tggagccggc cgagggcgct gagcgccgag cccgtctcct cgcggtcctt gcgcaggagc 47820gggacgagga cggcgtcctc accggcgctc tcgcgcgcca tgccgctgag cacgccgtcg 47880gggcccacct ccacgaaccg ggtgacgccc tcttccgcga gggcccggac accgtcgtcg 47940aaccggacgg cctcgcggac gtgggtgacc cagtactcgg gctcgtccac ctgtcccggt 48000tcggcgagcg tgccggtgat gttcgagacc agcgggatcc tcggcgccgc gaaggacagc 48060ccctccacca cctcgcggaa cgcgtggagc atcggctcca tcagcgggga gtggaaggcg 48120tgcgagacgc gcagccggct gacccggcgg cccagctccc ggaagacctc ggcgaccgac 48180tcgacggagg cctcggagcc cgagacgacc accgaggacg ggccgttgac ggcggcgatg 48240gacaggttgc cggtgaggtg cggcagtacc tcggcgcacg tggcctcgac cgccgccatg 48300gcgccgccct cgggcagcgc ctgcatcaga cggccacggg cggcgacgag cgcggcggcg 48360tcggcgaggg tgaacacccc ggccacgtgc gcagcggcga cctcaccgat ggagtgaccg 48420gccacgaact ccggccgcac cccccacgac tcgaccagcc ggaacaacgc cacctcgacg 48480gcgaacagac ccggctgggc gtacgccgtg cggttcagga gttcggcgtc gtcgccccac 48540acgacctcgc ggagcgggcg gtccaggtgc tcgtccagct cggcgcagac cgcgtcgaac 48600gcctcggcga ggacggggaa ccgttcgtac aggtcacgcc ccataccgag ccgctgggag 48660ccctgcccgg ggaagaggaa ggcggtgcga ccgttcccgg ccacgccttc cgcgacggcg 48720gcgtcgggag ccccggcggc cagcgcgcga agggacgtca gcgccgtttc caggtcggcg 48780cccaggacca ccgcgcggtg gtcgaagagg gtgcgctgcg tggccagaga gagcgccacg 48840tcccgtacgt ccgccggcgc gtcggtgagg aagtcggcca ggcgggcggc ctgggcgcgc 48900agtgcctcct cggtacgagc cgacaggacc cacggctgga caccggacga tgcctccgtc 48960ccgtctgcct ctcccgggcc cgacccggtg accggctcgg gctgttcgag gatgacgtgg 49020gcgttggtgc cgctgatccc gaacgaggac acaccggccc ggcgagcccg ctcctccccc 49080ggccacaccc gctcaccggt cagcaactcc acagcaccgg ccgaccagtc gacgtgcgac 49140
gacggagcat ccacatgcag cgtgcgcggc agcctgccgt gacgcatcgc catcaccatc 49200ttgatcacac ccgccacacc ggcggcagcc tgcgtgtgcc cgatgttgga cttgacggaa 49260ccgagcagca acggccgctc ctcgtcccgg ccctgcccgt acgtcgccag gagcgcctgc 49320gcctcgatcg ggtcacccaa cgtcgtcccc gtaccgtgcg cctccacgac atccacatca 49380ccggccgaca gaccaccact cgccagagcc tgacggatga cccgctgctg ggaaggaccg 49440ttgggcgccg tcagaccgtt cgacgcaccg tcctggttca ccgccgaacc acgcaccacc 49500gccagcacac gatgaccgtt gcgccgggca tccgacagac gctccagcac caccatgccc 49560acgccctcgg accagcccac accgtcagcc gagtccgcga acgaacggca ccgcccgtcc 49620gccgacagac caccctgacg cgagaactcc acgaacgtcg atggcgtcga catcaccgtc 49680acaccaccgg ccaccgccaa cgagcactca cccgaacgca acgcctgcgc agcccagtgc 49740aacgccacca acgacgacga gcacgccgtg tccaccgtca ccgccggacc ctcgaacccg 49800aacgtgtacg aaacacggcc cgaggccaca cttccggcac taccgctgcc ctggtacccc 49860tcgtactccg gggaggccag caggttggcg tagtcgtggt acatgacgcc ggcgaagaca 49920ccggtctggc tgccgcgcag ggagaccggg tcgataccgg accgctcgat cgcctcccac 49980gtcgtctcca gcagaagacg ctgctgcgcg tccgtggaga cggcctcacg cgggctcatc 50040ccgaagaact ccgggtcgaa ctcgggcgcg tcgtgcagga agccaccaga acgcgtgtag 50100gaggtgcccg gcacagcgcg gtcggggttg tagagcgcgt ccacgtccca gcctcggtcc 50160gaggggaagt ccgagaccgt gtcgacgccc tcggagacga cgcgccacag gtcctccggc 50220gaggagacgc caccggggta gcggcaggcc atgcccacga tcacgatcgg gtcctcggcc 50280accgacggca gcgccgacac cggcaccacc gccgccggct ccaccccacc gaacagttcc 50340tccagcaggt accccaccaa cgcgttgacc gtcggatagt cgaacaccac cgtcgccggc 50400aaccgcaacc ccgtcgactt ccccaaccgg ttccgcaact ccaccgcagt caacgaatca 50460aaacccagat cctgaaacgc acgccccgga tccaccgact gcacacccgc atgacccaac 50520accacagcga cctgaccacg caccagatcc agcaacacct cacgacgctc atctacgccc 50580aacgccccaa gacgctgcac cagaccagcc gccgccaccg aaccacccgc agccgcacga 50640cgaccacgcc gccgcaccag accccggaag agagccggta cgtcctcctc accgcgcagg 50700gcggccaggt cgaggcggac ggggaggacg accggctcac ccgaggccag ggccgcgtcg 50760aagagcgcgg tcccctgctc ggcctcgacc ggcggcagac cggaccgggc gatacgccgc 50820cggtcagtct ccgacagtcc tcgtgtcatc cccgcgtcct gcgcccacgg accccacacc 50880agcgacacac cgggaagacc cagagaacgc cggtactcca ccaacgcgtc caggaacacg 50940ttccccgccg cgtacgcgcc ctgccccgca ctcccgaacg tccccgccac cgacgagaac 51000accacgaacg cctcaagatc aaggccacgc gtcgcctcgt gcaggttcca cgccgcgtcc 51060accttcggac gcaggaccgc agcaacccgc tccgccgtca gcgactcggc catgccgtcg 51120tccaggacgc ccgccgcgtg cacgacggcg cgcaccgggc ggtcggccgg tactccggcc 51180agcagggcgt cgagcgcggt gcggtcggcc aggtcgcagg cggcgaccga cacccgggcg 51240ccgaggccgg tcagctccgc ggtgaaggcg tcgacgccct cggcggcggc accgctccgg 51300ctcacgagca gcaggtcccg caccccgcgc tcgaccacca ggtgccgggc gacgatccgc 51360cccagcccac ctgtgccgcc ggtcagcagc acggtgccgg agccgagacc ggccgacgat 51420cccgcggtct cctctccccc ggcgtccggc gtcgtgcgga ccaggcgcgg gacgcacacc 51480tcgctgtcca gcacggccgt ctccggctcg tccacggcga gggccgcgaa cagccgcccc 51540gcgtccgtac ccgccgggag gtgcagcagg ccgaagcggc cggggtgttc cagcgccacg 51600gaacgcacca gaccgcgtac gggcgcgcta gtcagagcgg aggcgggctc cgtgacgtga 51660ccgcctcggg tgatgaagac cagccgggag ccggcgaacc gctcctgcgc cagccactcc 51720ttgaccaggg cgagcgccca ggcggccgag gcgtgcgcgg agcccacgac gccctcggtg 51780
tccggggcgg gcggaacggc gaccacgacc gtctccggca ccgcctcggc cgcggccagc 51840tcctgcaggt cggcgcggac ggctgcgccg tcgcccgcga gatcggcgag gccctcgccg 51900aggaggacga cggatcccgc tgcgggagtc gcacgcgtgc cccgggccgg gacccactcc 51960acgcggtaca gcggttcggc ggtgtcggcc gcgcgggcac cggcggcggc gcggacggtg 52020agggagccga ccgaggcgac gggggctccg gtcgggtcgg ccacgtgcac ggccagcgtg 52080tcgtcaccgg tccgggtcag ccgggtccgc aggacggagg cgccggacgc gtgcagggtg 52140gcgttctccc agacgaaggg caccccgccg ggcgcgtcgt cgccgccgaa ggcgaaggag 52200gcgtgcaggg cggcgtcgaa gagggccggg tgcaggccga aggcggtggc gtcgacaccg 52260tcgggcaggg cgacctcggc gaacacctcg tcgtcccggc gccaggcggc ccgcagcccc 52320tggaagacgg ggccgtagcg cagtccggcc tcggccaggc tctcgtagca gtcggtcacg 52380tcgaccggca cggcgtcccg tgggggccat acggaggcgt cgaagccttc ggccgccggg 52440tccgcgtcga tggtctccgg ggcgagtacg ccctgggcgt gcaggctcca gggctgccgt 52500tcgtcgccgt cgtcccggga gtggatcgcg acggtgcggc ggccggattc gtcaggttcg 52560ccgacgccca cctggacgcg cacggcgccg cgctcgggca gcaccagggg cgcggacagg 52620gtcagctcct cgaccgatcc gcagcccgcc tcgtcggcgg cgcgcacggc catctccagc 52680agggccgtcc ccggcaccag cacggcaccc tggacgacgt ggtcggcgag ccacgggtgc 52740gaccgtgtcg agaggcgtcc ggtgaggatc agaccgtcgg cgccggccag ctccacggcc 52800gcgccgagca acgggtgccc ggccgcaccg agtccggcga ggcggatgtc cccggtcccg 52860gtggtcgttc ccgtgggcca gtagtgctcg tgctggaacg cgtaggtggg caggtcgacc 52920gcgcggccgc ccgtgccgtc gaggacggcg ccccagccgg cggagacgcc ccgcacgtgc 52980agttcggcga gggcgctcag tgcgacggcc acttcctcgc ggtccttgcg caggagcggg 53040acgaggacgg cgtcctcacc ggcactggcc cgcgccatgc cactgagcac cccgtcggga 53100cccagctcca cgaaccgagt gacgccctct tccgcgaggg cccgcacacc gtcgtcgaac 53160cggacggcct cacggacatg ccgcacccag tagtccggcg cggtcagttc acccggctcg 53220gcaatccggc ccgagacgtt cgagacgacc ggcaggcgcg gctcggcgaa cgacagatcc 53280cgcaggacct cgtggaacgc gtcgagcatg ggctccatga gcggcgagtg gaaagcgtgc 53340gacacgcgca gtcggctgac ccgacgcccc agctcacgga acacctccgc caccgcctcg 53400acggcttcct cggcgcccga gacgaccacg gacgacggac cgttgaccgc agcgaccgac 53460acaccctcga cggactccag acgcggcagc acctccgcct ccgacgcctc cacagccacc 53520atcgcgccgc cctccggcaa cgcctgcatc aaccgcccac gagcagccac cagaacacac 53580gcgtccgcca gcgagaacac acccgccaca tgcgcagccg ccacctcacc gatcgaatga 53640ccggcaacga actccggccg cacaccccac gactcgacca gccggaacaa cgccacctcg 53700acggcgaaga gaccggcctg cgcgtacgcc gtcccgtcca gaaccgacgt gtcgtcgccc 53760cagaccacct cacgcagcgg gcggtccagg tgcgcgtcga gtccggcgca gaccgcgtcg 53820aacgcctccg cgaacaccgg gaaacgctcg tacagaccac gccccatccc cagacgctgc 53880gacccctgcc ccgagaacag gaacgcggtg cggccgggct cgacggcgcc gaggacgacg 53940gcggggtcgg gctcaccgac gcagacggcc gcgaggccac ggacggcggt ctcgcggtcg 54000tccgtcagga ccacggcgcg gtggtcgaag agggaacgct gcgtggccag ggagagcgcc 54060aggtccacgg gacgcaggcc ggggtggccc tcgacctggg ccagcagccg ggcggcctgg 54120gcgcgcagcg cctcctcggt acggcccgac aggacccacg gcacgatgcc gggggcgagg 54180gcggcgatcc cgtccccgcc ggtctcgggt tccgcgaccg gctcgggctg ttcgaggatg 54240acgtgggcgt tggtgccgct gatgccgaac gaggacacac cggcccggcg cagccgctcc 54300tcccccggcc acacccgctc accggtcagc aactccacag cgcctgccga ccagtcgacg 54360tgcgacgacg gagcatccac atgcagcgtg cgcggcagcc taccgtgacg catcgccatc 54420
accatcttga tgacacccgc gacacccgcc gcagcctgcg tgtggccgag gttcgacttc 54480accgaaccga gccagagcgg ccggccctcc tcacgctcct gcccgtacgt ggccaggagt 54540gcctgcgcct cgatcgggtc acccagcgtc gtccccgtgc cgtgcgcctc cacgacgtcc 54600acatcaccgg ccgacagacc accactggcc agagcctgac ggatgacacg ctgctgggag 54660ggaccgttgg gcgccgtcag accgttcgac gcaccgtcct ggttcaccgc cgaaccacgc 54720accaccgcca gcacacgatg accgttgcgc cgggcgtcgg agagacgctc cagcaccacc 54780atgcccacac cctcggacca gcccacaccg tcagccgagt ccgcgaacga acggcaccgc 54840ccgtccgccg acagaccacc ctgacgcgag aactccacga acgtcgtcgg cgtcgacatc 54900accgtcacgc caccggccac cgccaacgag cactcacccg aacgcaacgc ctgcgcagcc 54960cagtgcaacg ccaccaacga cgacgagcac gccgtgtcca ccgtcaccgc cggaccctcg 55020aacccgaacg tgtacgagac acggccggac gcgatgctgg gtgcgctgcc gttgctgcgg 55080aagccctcat accgctcgtc ggcgagcatg ttgccgtagt cgttgtacat gacaccggcg 55140aagacgcccg tctggctgcc gcgcagggag gccgggtcga taccggaccg ctcgatcgcc 55200tcccacgtcg tctccaggag aagacgctgc tgggagtcgg tcgccaccgc ctcgcgcggg 55260ctcatcccga agaactcgga gtcgaaggct ccggcgtcgt gcaggaagcc accggccttg 55320gtgtaggtgg taccggacgc gtcccggtcg gggttgtaga gcgcgtcgac gtcccagcct 55380cggttgacgg ggaactcgga gacggtgtcg acgccttcgg agacgacggt ccagagatcc 55440tccggcgagg agacaccgcc cgggtagcgg caggccatgc ccacgatcac gatcgggtcc 55500tcggccaccg acggcagcgc cgacaccggc acgaccgacg caggctccac cccgccgaac 55560agctcctcca gcagataccc caccaacgcg ttgaccgtcg gatagtcgaa caccaccgtc 55620gccggcaacc gcaaccccgt cgacttcccc aaccggttcc gcaactccac cgcggtcaac 55680gaatcgaaac ccagatcctg aaacgcacgc cccggatcca ccgactgcac acccgcatga 55740cccaacacca cagcgacctg accacgcacc agatccagca acacctcacg acgctcatct 55800acgcccaacg ccccaagacg ctgcaccaga ccagccgccg ccaccgaacc acccgcagcc 55860gcacgacgac cacgccgccg caccagaccg agcatcatcg ggtgcaggtc accctgcccc 55920cgcagggcgg ccaggtcgag gcggacaggg aggacgaccg gctcaccgga ggccagggcc 55980gcgtcgaaga gcgcggtccc ctgctcggcg gtcagctcgg gcatgcctgc gcgggcgacg 56040cggtgccggc tggtcccgga gagggtgccg atcatgccgg cgtcctgggt ccacgggccc 56100caggcgagcg aggtcgcggg caggccgagg ctgcggcggt ggcgtaccag ggcgtcgagg 56160aaggtgttgc cggccgcgta gttggcctgg ccggggccgc cgaggatgcc ggacatcgag 56220gagaagacga cgaaggcttc gaggtcgagg gcgcgggtgg cctcgtgcag gttccacgcg 56280gcgtccgcct tcgggcgcag gacgcgggtc acgcgctcgg ccgtgagtga ttccaccgtg 56340ccgtcgtcca gcaccccggc ggagtggacg accgcgcgca ccgggtggcg ggcgaccagc 56400tccgcgacgg cggcggggtc ggagacgtcg cacgcctcga cggtcacgtg cgcaccggtc 56460tcggacagtt cggcgaccag ttgcccggcg ccgtcggcgg cggcgccgct gcggctcacc 56520agcagcaggt cccgcacccc gcgctcgacc accaggtgcc gggcgacgat ccgccccagc 56580ccacccgtac cgccggtcag cagcacagtg ccgtcgatgc tgtcccagcg cgggcccgtc 56640gtggtgccgg aggtgatccg ggccagtcgc ggggcgagca cctcgccgtc gcgtgcggcg 56700gtctccggct cgcccgtggc gacggcggcc agcacggtcg cggtgtcggt gccgcgtccc 56760acctccatca ggccgaagcg gccggggtgc tcggagacgg cggagcgcac caggccccgg 56820agggccgcgt cggccaggtc gccgccgtcg acggcgtccg aggtgacgaa gaccaggcgg 56880gagtcggtga accgctcctg cgccagccac tcgcgcacca gctccaggcc acgggcggcc 56940agggcgtgga cggcgtccac caccgcgtcg ggccgcgtcg cggcgtcggg acgcacccgg 57000accagcaccg tcgccggtac ctcttcggcg gcggccaggt cctccaggtc ggggaaggcc 57060
acgaacccgc cgtccgacag cccgtcggcc cggaggactc ccgcaagggc cgtggacgcg 57120tcggaatccc cgaggacgcc gaccggaccg gtgccggccg cgtcaccagg tgcctcggtc 57180cggacccagt ccacccggaa gagggcgtca cgggtcaggg cgtcgacacc ggtggcgtcg 57240ggggcgaccc ggcggagcac caggttctcg acgctggcga cgggcgcacc ggtggggtcg 57300gccacggcga ccgtcacggt ctcgtcgccg gtccgggtca tccggacccg gacctcccgg 57360gcgccggtgg cgtgcaggca caccccttcc caggagaacg ggaccacacc gtgcgcgaga 57420ccacccaggg ccgagacgtg gagggaggcg tcgaggagcg ccgggtggag gccgtaggcg 57480gtggcgtcgc gctcggcgga gtcgggcagg gcgacctcgg cgaagacgtc gtcgccccgg 57540cgccaggcgg cccgcagacc ctggaagagc ggcccgtacc ggtagccgcc ctcggccatg 57600cgctcgtaca ggccggtgag gtcgagggat tcggcgtcgg cggggggcca caccgaggtg 57660tcgaactcca cggtgcgctc cccgggagcg aggacaccgg tggcgtgctc ggtccagggc 57720tggtcgtcgg cgccctcggc gcgggagcgg gccgcgagga cgcggcggcc ggcggcgtcg 57780gcggggccca cgtgcaggtg gagctggacg ccgccctgct cgggcaggac cagcggggcg 57840gcgagggtca gttcctcgac acggtcgcag ccgacctcgt cgccggcgcg gaccgcgagt 57900tccacgaagg ccgtgccggg cagcagcgcg ttgcccatga cgacgtgctc gccaagccag 57960gggtgggtgc gcagcgacag ccgggtggtg agcaggaggc cgccggagtc ggagagttcg 58020acggcggcgc tgagcagggg gtgcccggcg gcgcgcagtc cggcggactc cacgtccccg 58080gcgggtgcag gtgcgggcac gggccagtag tgctcgtgct ggaaggcgta cgtgggcagg 58140tcgacgaggc gggcgccggt cccggcgagg acggcggtcc agtcgacggg cgcaccggtg 58200gcgtggaggc ggcccagcgc ggcgagcgcg gtgacctcct cgtcacggtc cttgcgcagg 58260agcgggacga ggacggcgtc ctcaccggca ctgacccgcg ccatgccact gagcaccccg 58320tcgggcccca gctccacgaa ccgcgcgaca ccctccgccg ccagagcctg gacaccgtcg 58380tcgaaacgca cggcctcgcg gacatgccgc acccagtagt ccggcgtggt cagttcaccc 58440ggctccgcga tccggcccga gacgttcgag acgaccggca gcgaaggctc cgcgtacgac 58500aggccggcca gagcctcacg gaagtcaccg agcatcggct ccatcagcgg cgagtggaaa 58560gcgtgcgaca cccgcagcct gctgacccgg cgtccctgct cacggaacac ctccgccacc 58620gcctcgaccg cgtcctcggc gcccgaaacg accaccgacg acggaccgtt gaccgcagcg 58680accgacacac cctcgacaga ctccagacgc ggcaacacct ccgcctccga cgcctccaca 58740gccaccatcg cgccgccctc cggcaacgcc tgcatcaacc gcccacgagc agccaccaga 58800acacacgcgt ccgccagcga gaacacaccc gccacatgcg cagccgccac ctcaccgatc 58860gaatgaccgg caacaaactc cggccgcaca ccccacgact caaccagccg gaacaacgcc 58920acctcgacgg cgaagagacc cgcctgcgcg tacgccgtcc cgtccagaac cgacgtgtcg 58980tcgccccaga ccacctcacg cagcgggcgc tccaggtgct cgtccaaccc ggcgcacacc 59040gcgtcgaacg cctccgcgaa caccgggaaa cgctcgtaca gaccacgccc catccccaga 59100cgctgcgacc cctgccccga gaacaggaac gcggtggagg cgctgcggac ggcgtcctcc 59160aggacagcgg ggtggggttc gtcggcggcg agggaggtga gagctgcttc ggtggttccg 59220gggtcggtgg cgaggacgac cgcgcggtgc gggagcgggg cgcggctggt ggcgagggag 59280agggcgatgt ccgccgggcg cggggactcg gggccggtgt ggtcggtgag gtgggccagg 59340agtcgtgccg cctgggcgcg tagcgcggtc tgcgaacggg cggagaggag ccagggggtg 59400accgccgggt cggtggccgt caccggttcg gcgggggtgg cgtcctgagg ggtgtcctcg 59460gccccggcgg gttcgggggt ggcctgttcg agcaggacgt gggcgttggt gccgctgatg 59520ccgaaggcgg agacgccggc ccggcgggga cggtcggcgt cgggccagtc gacggcctcg 59580gtgaggagtt cgacggcgcc ggccgtccag tcgacgtgcg gggtgggggt gtcgacgtgg 59640agggtggcgg gcagcaggcc gtgccggatg gccatgacca tcttgatgac gccggcgaca 59700
ccggcggcgg cctgggcgtg gccgatgttc gacttgatgc cgcccagccg cagcgggcgg 59760tcctccgggc ggttctggcc gtaggtggcg aggagtgcct gggcctcgat ggggtcgccc 59820agggtggtgc cggtgccgtg cgcctcgacg gcgtcgacct ggtcggcggc gaggcgggcg 59880tcggcgagcg cggcctcgat gacgcggcgc tgggagggcc cgttgggcgc ggtgaggcgg 59940ctgctggcac cgtcctggtt gacggcggtg ccgcggacga cggcgaggac ggggtggccg 60000ttgcggcggg cgtcggagag ccgctcgacg agcagcacgc cgacgccttc gccccaggaa 60060gtgccgtcgg cggcctcggc gaagggcttg cagcggccgt cggtggcgag gccgcgctgg 60120cgggagaact cggtgaagag ttcggtggtc ggcatcatgg cgacgccgcc ggcgagggcg 60180agggagcact cccccgcgcg cagggcgcgg accgcgaggt gcagggcgac cagggaggag 60240gagcaggcgg tgtcgacggt gaccgccggg ccctccaggc cgagggtgta ggagatccgg 60300ccggagagga cgctcatggc gccgccggtg aggttgtggc cctcggtctc ctcggcggtg 60360cgggccaggg agccgtagtt ggtgtcggcg ccgccgacga agacgccggt gtcggtgttg 60420cgcagggagg cggggctgat gcgggcgcgc tccacggcct cccaggcggt ttccaggagc 60480aggcgctgct gggggtccat ggcgagggct tcacgcgggg cgatgccgaa caggtccgcg 60540tcgaagtcgg ccgcgccttc gaggaagccg ccctcacgga catagctctt gccgggggtg 60600cccgggtccg ggtcgtagag gccgtccagg tcccagccac ggtccgcggg gagggggccg 60660atggcgtcgg tgccgtcggc gaccagctgc cacaggtcct ccggggagcg gacaccgccg 60720gggaaccggc aggccatgcc gatgacggcg atgggctcgc tctcccgtgc ctcgaccgtc 60780tccagccgct gccgggtgcg gcgaaggtcg gtggtgacct tcttgaggta gtcgcggagc 60840ttggcttcgg agcttgccat ggggtggatc ctctgaattc gggaaggacg cggtggccgg 60900ggcgggggac gggagcggtg gccgccggcg cgctcgtgga cgcgccggcg gccaggcgcc 60960gtcaggcgag cccgagttcg ttgtcgatga ggtcgaacat ctcgtcgtcc gtggtggtgt 61020cgaggtcgga gtcgtcgtcg ggctcgtcgg cgtcctggga ggtggcgtcg agcttccacc 61080cgagggcctt gagtcgctgc gccaggcggg cgcggtcctg gtcgtcgagg gcggccccgc 61140cgacggcggc ctccagggcg gccagttcgg cgaagacggc ctggtcgggt tcgccggcgc 61200ctgtgccgag ttcggtctgg aggtggcgaa cgagggcggc cgggtccggg tggtcgaaga 61260tcagggtggt gggcagggag agcccggtgt cggcgttgag ccggttgcgg agttcgaccg 61320cggtcagcga ggacatcccg aggtcgagga agccgcggtc cgcgtcgagg gacgcggcgg 61380aggcgtggcc gaggacggcg gcgaggtgac cgaggaccgt acggtgcagc agcgcccgct 61440gctgggggcc gctgaggccg gccagccgct cacggaggtc cgccggagcg ccggtggccg 61500cgccgggccc ggagtcggcg gcgcgtcgta cggccgggcg tgccagggcc cgcagcagcg 61560ggggcacctc gtcggcgcgc agggtgcggg tgtcgagccg tacggggacg aggtgggcgg 61620tgtccagggc ggtgctcgcg tcgaggaggg cgagcccctg tccggcggtg agggggcgga 61680tgccggagcg ttccaggcgg gccagaccgg tggtgtcgag ggcgccggtc aggtcgctgc 61740ggtcctccca gaggccccag gcgagggagt gcgcgggcag gccgctcgcc cggcggtgct 61800gcgcgagggc gtcgaggaag gcgttggcgg ccgcgtagtt ggcctggccg gggccaccgg 61860cgacgcccgc gtaggaggag aacatcacga aagcggtgag tttggtgtcg cgggtgagct 61920cgtggaggtg ccaggcggcg tcggccttgg gccgcaggac gtgggcgacg cggtcggcgg 61980tgaggccgtc cagtgtgccg tcgtccagga caccggccgt gtggacgacg gcggtcaggg 62040ggtgttcggc cggtacggcg tcgaggacgc cggccagttg ctcgcggtcg gcgacgtcgc 62100aggcgacgat ctctgcctcg gcgccgagcg cggcgagttc ctcgcgcagg gccgcagctc 62160cgggggcgcc gggcccgcgg cggctggtga gcaacaggtg gcgtacgccg tggcgggtga 62220cgaggtgacg ggcgacgagg gcgccgaggg tgccggttcc gcccgtgatc agcacggtgc 62280cggcgccggt tccctcgccc cacggagcgg tggcggcggg cgcgggggcg ccctccgggt 62340
gaccggcggg gaccaggcgc ggcacggtga cggtgccggc ccgtacggcg aactggtgct 62400cgccggtgcg cgcggccgcc gcggcggcgg cgatcagggc gtcggcggcg ctgtcctcgt 62460cggtgtcgcc gggggcaggg tcgaggtcgg ccaggaggaa gcgtccgggg tgctcggtct 62520gggcggagcg gagcaggccc caggcggtgg cgtcggcgag gccggggacg gtgtcgtccg 62580ggccggtggc gacggcgccc tcggtgacca ggaggagccg ggcgtcggcc aggcgcggct 62640cccggagcca ggcggtggac agcgttaggg cacgggccgc agaggtgtgc gctgccttcg 62700tcgggtcctc gccgctgtcg tggccgtgga aggcgacgac cagggcggga ggtgcggcct 62760cgtcgttgtc gagggcggtg gtgagggcgg ccaggtcggg gtggagctgg acggtggcgc 62820cgctgcgttc gagggcggcg gtgagccgtc cgggggcacc gatgacggcc cacggaccgt 62880gtggttcggg ggtggcggag gaggttgtgt cggtccacgg gagccaggcg acccggtgcg 62940gggcgtcggg gccggcggtg gcggggagcc ggtcggggcc ggccgagcgg gtcacggcgg 63000tgccggtgag gacgaggtcg cctgcggcgt cgcgggcttc gacgctccag gtggcgtcgt 63060cccccggggt gaggcgtacg cggagcctgc tggcgccggt ggcgtgcacg gtgacgtccc 63120gccaggcggc gggcaggacc ggtgcggtcg cctcctcggt ggtcgcggcg agggcgagcg 63180gggtctgcag gagcgccggg tggaggcgga agcgcgccgc gtcggcgtgc gcggcgtcgg 63240gaagtgcgac ctcggcgagg acgtcggtgc cggcgcgcca cagggagcgc accgtgtcga 63300agggcgcggc gaggtcgagg ccgcggtcct cggcgagggc gcgcacgggg gcggggtcgg 63360ccgcgacggc gtcggcgggc ggccaggttc cggaagcctg cggggtggtg gtggggcggt 63420cggtgaggtc gccggtcgcg ttccgcgtcc agggggtggc gggctcaccg gcgtcggcgg 63480cgtggagcct tgagtggacg gagaacggcc gggagtcggt gtcggcggcg gagacggtga 63540gctggatctc gacgtgcccc tcctcgggca cgacgagcgg gctgtgctcg tcgagtgcgg 63600cgaccacggg caggccggtc tcgtcgccgg cccggaccgc ccagtccagc agggcggtgg 63660tggggacgac ggcggcgccg tgggtccggt gggcgtccag ccagggctgg gtggtgaggg 63720acagggagcc gctgaggacg gctccgccgt cggccaggcg gacgacggag tcgaggccgg 63780ccgggtcgcc ctggggggtg ccggcgtcca gccagtaccg ccggcgctgg aaggcgtagg 63840tcggcaggtc gacggcggtg ccgtgggaca cggcggggcg ccagtcgacg gggacgcctt 63900cgacgtgggc ggcggcgagg gcggtcagga cacgggcggg gccgccgtcg tcgcggcgca 63960gcgagccgag ggccaccgcg tcggcgccga gggcggcgac ggtgtcctcg atgccgacgg 64020tgagcaccgg gtgcgggccg cactcgacga agacgtcgtg gccctgctcg acgagggccc 64080gcagggactc ctccaggcgg acggtgccgc gcaggttgcg gtgccagtag gcggcgccga 64140gtccggtgcc ggggtgccag tccgcggtga cggtggagaa gaaggggatg tcgccgtcgc 64200ggggctcgat gccgtcgagg gcggcgggca ggctctcggc gagccggtcg acgtgggccg 64260agtgggaggc gtagtcgacg gggacgcggc gggcgcggac gccctgttcc tcgcaggcgg 64320cgaggagggt gtcgagcccg ccggtgtcgc cggagaccac cacggaggag gggccgttga 64380cggcggcgac gcagacgccg ggcagatcgg cgaggagggc ggtcacctcg tcgtgcgggg 64440cggtgacggc caccatgccg ccgtgtccgg agagttcgcg ggcgatggcc cggctgcgga 64500ccgcgacgat cctggcggcg tcggcgaggg agagggcccc ggcgacggcg gccgcggcga 64560tctcgccctg ggagtggccg aggacggcgg ccggacgcag gccgtgggcg cgccacacct 64620cggcgagggc gaccatcacg gcccaggtga cgggctggac gacgtcgacg cggtcgaggg 64680cgggggcacc ctcctcctgg cgcaggacgg cggtcaggga ccagtcgacg tacggggcga 64740gcgcctcctc gcaggcggcg acgcggtcgg cgaaggcggg gctggcgtcg aggagttcgg 64800cgcccatgcc ggcccactgg gagccctgtc cggggaagac gaggacggtc cggcggtcgc 64860gaccggcacc ccggccgccg gtcaccacgt gggcggcggg actgccctcg gcgagggcgg 64920cgaggccggc gcggaagtcg ccggtggcgc tcccgacgac gacggcccgc ttctccagct 64980
gggcgcggcg cgtgaccagg gtgtgggcga tgtcggcggg gtcggtcccg gggtgggcgt 65040cgaggtgggt gaggagggcc ttggcctggc cgcgcagggc ggcctcgctg cgggcggaca 65100gcacccacgg caggggcgcg gggcgggtct ccgccgcgtc cggcgccggt gggctccggc 65160tcgccgccgt ccgggccggg ggcggcgggg gccctcctcc aggatgacgt gggcgttggt 65220gcccgctgat gccgaaggag gagacggcgg cgcggcgcgg gcgggaggcg ggcgcctcgg 65280gccacgcggt ggcctcggtc agcaggcgca cgccggagtc cgcccagtcg acgtgcgggg 65340tcggcgcgtc aatgtgcagg ctctcgggga gcaggcggtg gcgcagggcc agcaccatct 65400tcatcacccc ggcgacaccg gccgcggcct gggtgtggcc gaggttggac ttgacggagc 65460cgaggaacag cgggtggccg ccgtcccggt cgcggccgta cgtggcgagg agcgcctggg 65520cctcgatcgg gtcgccgagg cgggtccccg tgccgtgcgc ctcgacgacg tcgacctcgg 65580cggcgccgac acccgcgtcg gcgagggcct ggcggatcac ccgctgctgg gcggggccgt 65640tgggggcgct gaggccgttg ctggcaccgt cctggttgac ggcggagccc cggaccacag 65700cgaggacctg gtggccgttg cggcgggcgt cggagagccg ctcgacgagg atcaggccga 65760cgccctcgga gaagccggtg ccgtcggcgg cctcggcgaa gggcttgcag cggccgtcgg 65820cggcgaggcc gcgctgccgg gagaagtcca cgatgatctc gggcgaggac atgacggtga 65880cgccgcccgc cagggccatg gtgcattcgc cgcgtcgcag ggcctgcagc gccatgtgca 65940gcgcgacgag ggaggaggag caggcggtgt cgacggtgac ggccgggccc tccaggccga 66000gggtgtagga gacgcggccg gagacgatgc tgttggcgtt gccgttgttg acgtatccgg 66060cgatctcgtc ggggacgtgg gtgacgcgct tgaggtagtc gctggaggag aggccggcga 66120agacgccggt gcggctgccg cgtaccgagc gggggtcgat gccggcgcgt tcgaaggtct 66180cccaggccgt ctccaggagg aggcgctgct gcgggtccat ggcgagcgcc tcgcgggggg 66240agatgccgaa gaagtcggcg tcgaagtcgg cggcgtcgtg gaggaagccg ccctcgcggg 66300tgcgggagct gccctggccc tcggggacgg agtcgtagag gcgggcgagg tcccagccac 66360ggtcgtcggg gaagggcgcg acggcgtcgg cgccgtcggc cagcagccgc cacaggtcct 66420cgggcgagtg gatgtcgccg gggaggcggc aggccatgcc gacgatggcg aaggggtcgt 66480cgtcgccggg cgcggcggga gcgggtgcgg gcgcggcggg gagcgcggcc ggggtgccgt 66540cggagagttc gccgtcgagg tgggcggcga gggcggcgac ggtcgggtgg tcgaagacga 66600gggtggcggg cagccgcaga ccggtggcgg cgctgaggcg gttgcgcagt tcgacggcgg 66660tcagcgagtc gaagccgagg tcgcggaagg cgcggccggc gtggaacggc tcggtgtcgt 66720cgtggccgag gacggcggcc gcgtgggcac cgaccaggtc gcgcagggtg ctgagccggt 66780cctcggcgga acggccggcc aggcgggcgc gcaggccgtc ggtaccggtg gcggcgccgg 66840cctcggcggt gcgccggacc gggccccggt acagggagcg cagcaggggc gggacctcgt 66900cgccggatgc gcgcagctgt ccggtctcca ggcggaccgg caccaggtgg gggcgctgct 66960cggtgagggc ggcgtcgaag aggaccatgc cgtcggcggc ggagagcggc aggacgccgg 67020agcggcgcac ccgggcgagg tcggtgtcgt cgagctgtcc ggtcagggca ctggtgtcgg 67080cccacagacc ccaggagagg gaggtggtgg gcaggccgtc ggcgcggcgc cggtgggcga 67140gggcgtcgag gtaggtgttg gcggcggcgt agttggcctg tccggcgttg ccgaagacgc 67200cggcgcccgc ggagaacagc accaggtccg tgagatcgag gtcgcgggtg aggcggtgca 67260ggtggcgggc ggcgtcggcc ttcggggcga ggacggtgcg cagccggtcg ggggtgaggg 67320attcgaggac gccgtcgtcg aggacgcccg cggcgtggac gacggcggtc agcgggtgct 67380cggcggggac ggcggcgagc accgcggcga gggcgtcggc gtcggccgcg tcgcaggcgg 67440caagggtgac ctcggcgccc agctcccgca gctcggcggc gagttccccg gcgccggggg 67500tgtcggggcc ccggcggccg gtgaggacga ggtggcgcat gccgtggcgg gtgacgaggt 67560ggcgggcgag gatgccgccg aggacaccgg cgccgccggt gacgaggacg gtgccctcgg 67620
ggtcccaggc gcgcggcagg gtgagcacga tcttgccgta gtgcttggcc tgggccatgt 67680ggcggaaggc ttcgcgggcg tgccgcacgt cccaggtgac ggtgggcagg ggccgcaggg 67740ctccggaggc gaagagttcc gacaggtcgc ggaacatctc ctggatgcgg tcctcaccgg 67800cctcacgcag ttcgtacgcc tggtagaggg cggcggcggg gtggtcgcgg agaacctgcc 67860cgggatcgcg gacatcggtc ttgcccatgt cgacgaggcg cccgccgggg gcgagcagcc 67920gcagggaggc gtcgatgtag tcgccggcca gggagttgag gacgacgtcg acgccacggc 67980cgccgctggt ggtgcggaag cggtcctcga aggcgagggt gcgggaggag gcgaggtggg 68040cgtcgtccca gccgtcggcg cgcagcaggg tgcgcttgtg gtcgccggcg gtgccgtaga 68100cctcggcgcc ccagtggcgg gcgagctggg cggcggccat gccgacaccg ccggcggcgg 68160cgtggacgag gacggactcg ccggcgtcga ggtgggcgag gtcgcgcagt ccgtagtagg 68220cggtgaggaa ggcgacgggg acggaggcgg cctgcgccca ggtccagccg tcgggtacgg 68280gggcgagcat gcgggcgtcg gcgacggcgc ggggggcgaa gccgccggag accaggccca 68340tgacgcggtc gcccggggcg aagccggtga ctccctcgcc gacggcggtg accacaccgg 68400ccgcctcggc gccgaggagc acggcggggc cggggtacat gccgagggtg ttgaggacgt 68460cgcggaagtt gagtccggcg gctcgcacct cgatcaccac ctcgccggcg gcgggctcct 68520cggccgtccc gggggcggcg acgaggtcca gccggtcgac ggatcccttc tcggggatgt 68580cgaggcgcca gttcccggtc ccctcgggca cggtgagggc gtcggaggta cggggccgga 68640ccaggcgcgg cgcgtacgcg gcgccctggc ggagggcgag ttgcggctca ccggcggcga 68700gtgcggccgg cagggcgagc caggagtcgt cgtggatgtc gaggtccacc aggacgaggc 68760ggcccgggtg ctgggactgg gcggagcgca ccaggcccca gacggcggcg ccgggcaggt 68820cgtgggcggg gtcggcgggg ccggtgggga cggcgccgcg ggtgacgacg accagcggaa 68880cgccctcggt ggtctcgtcg gcgagccagc tctggacggt ggcgagcacg tcgtcgagga 68940cggcgtccgg gacgcccggc tcggcgtcgg gggcggcggc cgtgcccgac gcggcggccc 69000cggcgaacag caggacggcg tccggccggt gaccgttggc ctcggtgtgc tcggccagtg 69060cgccgggggt ggcgaaggcg ggcagtgtgg ttccgctgcc gtcgggtgcg gtgaccgggt 69120cggccggggt gccgaggacg gcccagccgg tggcggccgg gacggcggcg ggcggcgtga 69180ccggtgtcca gtcgaggcgg aacagccagt cgcgggcggc gcgggcggac ggatcgaggg 69240tgtccggcgc gatctcgcgt acggccaggg cgtcgatggt ggcgacgggg gcgccggtcg 69300ggtcggtgag ctggacggag atggcgtcct gccctgccgg ggagacgcgg acgcgcagcg 69360cggtcgctcc ggcggcgtgc aggcagaccc cgctccaggt gaacgggacg accgtgcgcc 69420cggcgctgcc gggggcgagg tgggcggcgt gcagggcggc gtcgaggagc gcggggtgga 69480tgccgaaccg tccggcgtca ccgcggacct gctcgggcag gacgacctcg gcgtagaggt 69540cctcgccgcg gctccaggtg gcgcgtacgc cctggaagac gggcccgaac tcgtagccac 69600ggccggcgag cagctcgtag aagccctcgg cggcgacggc ggtggcgccg gggggcggcc 69660aggaggtgaa ctcctcgggc cggtcgacgg cgggggcgcc gggctcggtg agggtgccct 69720gggcgcggag cgtccagagg tcctcggtgg cgccgtcggg gccggtggtg cgggtgtgga 69780cggtgacggg acggcggccg gaggcgtcgg gttcgccgac gccgatctgg acctgtgcct 69840cgccgcgttc ctggaggacc acgggggcct ggagggccag ttcctcgatc cggccgcagc 69900cgaggacgtc tccggcccgg atcacggact ccacgagcgc ggtcccgggg acgatcacgg 69960tgccggacac cgcgtggtcc gcgagccagg ggtgggtacg cagcgaccac cgtgccgtgg 70020ccaccaggcc gtcgccgccc gcgagttcga cggaggcacc gagcaggggg tgatcgggcg 70080agtcgaggcc ggccgagctg acgtcaccgc ccagctcggc gaaacgcggc cagtagtgct 70140gccgctggaa ggcatacgtg gggaggtcga cgtggcgggg ccgggtgccc gcgtaatgcg 70200tctcccagtc cacctcggca cccagcgccc acgcctcacc cagcgacagc gcgaaccgct 70260
ccagaccacc ctcgtcacgc cgcagcgtgc cgacgacacc ggcctgcacg ctcgcgtcct 70320cgatggtctc ctgcaccccg gtggccacca ccggatgcgc actcacctcg acaaacaccc 70380ggaacccgtc ggccagcaac gcacgcgtcc ccgcctcgaa ctccaccgtc ccccgcaggt 70440tccggtacca gtacccggcg tccagaccag cggtgtcgat cacgccaccc gacaacgtcg 70500agtagaacgg cacatcacac gagcgcggcg ccaccggcgc cagaagcccg gcaagctcct 70560cctcgatgcg ctccacatgc gcggagtgcg acgcgtagtc caccgccacc cgacgcgccc 70620gcacacccga cgcgtcacac gcggccacga actcgtccaa cgcctccgca tcacccgaca 70680ccaccaccga cgacggccca ttgaccgccg ccacactgat acgcccctcc cagcccgcca 70740gcagaccacg aacctcgtcc acgttccttg cgaccgacac catgccgccc agacccgaca 70800gcgcacgcaa cgcacgcgac cgcagcgcca ccacacgcgc cccgtcctcc aacgacagac 70860cacccgccac caccgcggcc gcgatctcgc cttgcgaatg accgatcacc gcacccggct 70920ccacaccctg agcccgccac acctccgcca acgacaccat caccgcccac aacaccggct 70980gcaccacatc cacccgctcc aacgacgcag caccctccac accgttcagc acatccagca 71040acgaccactc cgtgaacggc tccaacgccg ccgcacactc cgccacccga cccgcgaaca 71100ccggcgacga acccaacaac tccaccgcca tacccaccca ctgcgaaccc tgacccggga 71160acacgaaggc gaccttgccg gagccgtccc cgaccgagcc ttccaccaga cgcggggaag 71220gctccccggc ggcgagcgcg tccagcccgg cgagcagttc ctcgccgtcc cggccgacga 71280ccaccgcacg ccggtccatc gccacgcggg aggagaccag cgcatgtgcc acgtccagca 71340ccgacgcgtc ggacgtacgc accgacgccg ccagacgcgc cgcctgctcc cccaccgccg 71400cacgcgactt gcccgacacc atccacgacc ggacggtgtc ggccgagtcg tccgcggagg 71460gctcggtgaa cgggtcggcc ggctcgtcct cggcgggttc gggggccggg gcctcctcca 71520ggatgaggtg gccgttggtg ccgctgatgc cgaacgagga gacgccggcg cggcgcggac 71580ggccggtctc gggccagggg gtggactcgg tgacgaggcg gacggcgccg ctcgaccagt 71640ccacgacgga ggtcggccgg gtgatgtgga ggctcttggg cagggtgccc tgccgcatcg 71700ccaggatcat cttcatcatg ccggcgacgc cggcggcggc ctgggtgtgg ccgatgttgg 71760acttcagcga accgagcagc agcggccggg ccgggtcgcg gtcctggccg taggtggcga 71820ggatggcctg cgcctcgatg gggtcaccga gcggcgtgcc ggtgccgtgc gcctcgacca 71880cgtcgacctc gcccggggcg aggcccgcgt tggcgagggc ctggcggatg acgcgctgct 71940gggaggggcc gttgggggcg gtcagaccgt tggacgcacc gtcctggttc agggcggagc 72000cgcggaccac ggcgaggacc gggtgaccgt tgcggcgtgc gtcggagagc cgctccagca 72060ggaccaggcc gacgccctcg gcgaagccca tgccgtcggc gccctcggcg aaggccttgc 72120agcgcccgtc ggccgccagg ccccgctgcc gggagaactc gacgtagggc tcgggtgtgg 72180acatgacggt gacaccgccg gccagggcca tcgtgcactc gccggtgcgc agcgactgca 72240gggccaggtg gagggcgacc atggacgagg agcagccggt gtccatggtg acggcagggc 72300cttcgaagcc gtacgtgtac gacaggcggc cggaggcgat gctggaggcg ttgccggtca 72360tgaggtggcc ggcgagttgt tcctgggcgg cggcgccgcc caggcggacc tggtagtccg 72420tggtgatgct gccgacgaag accccggtgg cgctgttgcg cagggttccc gggtcgatgc 72480cggcgcgttc gaaggtctcc caggaggtct ccagcaggag ccgctgctgc gggtccatgg 72540ccagggcctc gcgcggcgag atcccgaaga acaccgggtc gaatccggcg acgtcctgga 72600tgaagccgcc ctcacggctg taggtggtgc cgtggcgctc ggggtccggg tcgtacaggg 72660cgtcgaggtc ccagccacgg tcctcgggcg gcgtggagac ggcgtccacc tcggcggcca 72720tgaggtgcca cagttcctcg ggcgtggtga cgccgccggg gaagcggcag gccatggcga 72780cgaccgccat cggctcgtcg gtgacgcccg cggagacgac gacgggcgcg gcgacggggc 72840gctcgtcgcc gaggagcagg gtgcgcaggt gggcgacgac cgccgtggtg ttggggtagt 72900
cgaagaggag ggtggtgggc agcttgacgt cggtggcggc ggtgatccgg ttacggacct 72960ccacggcggt cagcgagtcg aagcccaggt ccttgaaggc acggttgacg tccacggcct 73020ccggtgagga gtggcgcagc gcggtggcga cctcggcgcg gaccaggtcg gtgagcagcc 73080ggtcctgttc ggcgggggcg aggccgcgca gccggtcgcg cagggcgtgg gtgtcgggcg 73140cggtggcggc ggctgcctcc agggcgcggc ggacctcggg gaggtcgccg acgagcgggc 73200tgggccgggc cgcggtcatg gcggtgccgt aggcccgcca gtccacgtcg aggacggtgg 73260cgtgcgggac gccctcggcg acggcggcgc gcagcgcggc gacggcggtg acggggtcca 73320tcggggcgag gccgtcgtgg ctcatccggt cggcgagcac gtcggcggtg gccatgcccg 73380cgtcggccca cgggccccag gccacggagg tgacgggcag gccctcggcc cgccgctgct 73440cggctacggc gtccagatag gcgttggcgg cggcgtagtt ggcctgcccg gcgttgccga 73500ggacaccgac gatcgaggag aagaggacga acagggtgac gtcgtggtcg cgggtcagct 73560cgtgcaggtg ggcggtgccg agggtcttgg ggcgcaggac ggcttcgaag cggtccaggg 73620tgagggcgtc gaggacaccg tcgtccagga caccggcggc gtgcacgacg ccggtgaggg 73680ggaggccggc ggggagggag tccagcagac gggcgagctg gtcgcggtcg gccacgtcgc 73740agtcggcgac ggtgacccgg gcacccagct cctccagctc ggcgacgagt ccgactgcac 73800cggggctgcc gggtccgcgt cggctggtga gcaccaggtg gcgggcgccg gaggcggcga 73860gcgagcgggc cacgtgggcg ccgagcgcac cggtaccgcc ggtgatcagg acggtgtcgt 73920ggtcgcccca gcccccgatc ccgtcgtcgt cggccgcccg cacggcgagt tccgcgtcca 73980cgtcggtgac ccgggccggc tcgggcagcg gcattcgggc cagccgacgg gcgtacgcgc 74040cggaggagcg tacggcgatc tggtcctcgg ggccgccgag gagcgtggcg aggcgggcga 74100ggctgcgggc gtcggggtcg gcgggcaggt cggcgaggcc gccccaggcg tcggggagtt 74160ccagggcgac ggcgcgtccg aagccccagg tctcggcctg gacgggcgag gtcagccggt 74220cgcggctgtc gacggcgacg gcgccgcggg tcagcgccca cacggaggct tcggtgccgg 74280acagcgcgtg cagcagctcc ttggcggtgg cgaggccgcg cggtacggcg gggtggtcgg 74340ggtgggggcg ctcgtcgagg gcgaggagcg agaggacgcc cgcgatgcct tcggcgccgt 74400cgggctcggt gtcaccggtt ccggtgagca gggcggtgag cgcggcggtg tcctcgtcgc 74460cggtgaggtc gtggcgctcg acacgcaggc cgcgccccgt gagagccccc agcaccgcgt 74520gcacccaggg gtcgtcggcg tggccggcgg ggacgagggc gggccagcgg ccggcgggga 74580cggcgtcgtc ggcgccggtc agcgcggtcc aggtgacgcg gtggcgccag gtgtcgaggg 74640tggagcgcag ggtgcggcga cggcgccagg acgccagcgc gggcagcagc gtgccgaacg 74700cctccacgtc gtcgccggtg acctccagca cggaggagag ccgggtgaga tcgccgctct 74760cgacggccga ccagaagtcg ccgtcggcgg ggtcgccggt ggccgccgcc gcggccgact 74820cggcgggcgt cagccagtac cagtcacggc ggaacgggta ggtggggagg tcgacgtggc 74880ggggccgggt gcccgcgtaa tgcgtctccc agtccacctc ggcacccagc gcccacgcct 74940cacccaacga cagcgcgaac cgctccagac caccctcgtc acgccgcagc gtgccgacga 75000caccggcctg cacgctcgcg tcctcgatgg tctcctgcac cccggtggcc accaccggat 75060gcgcactcac ctcgacaaac acccggaacc cgtcggccag caacgcacgc gtccccgcct 75120cgaactccac cgtcccccgc aggttccggt accagtaccc ggcgtccaga ccagcggtgt 75180cgatcacgcc acccgacaac gtcgagtaga acggcacatc acacgagcgc ggcgccaccg 75240gcgccagaag cccggcaagc tcctcctcga tgcgctccac atgcgcggag tgcgacgcgt 75300agtccaccgc cacccgacgc gcccgcacac ccgacgcgtc acacgcggcc acgaactcgt 75360ccaacgcctc cgcatcaccc gacaccacca ccgacgacgg cccattgacc gccgccacac 75420tgatacgccc ctcccagccc gccagcagac cacgaacctc gtccacgttc cttgcgaccg 75480acaccatgcc gcccagaccc gacagcgcac gcaacgcacg cgaccgcagc gccaccacac 75540
gcgccccgtc ctccaacgac agaccacccg ccaccaccgc ggccgcgatc tcgccttgcg 75600aatgaccgat caccgcaccc ggctccacac cctgagcccg ccacacctcc gccaacgaca 75660ccatcaccgc ccacaacacc ggctgcacca catccacccg ctccaacgac gcagcaccct 75720ccacaccgtt cagcacatcc agcaacgacc actccgtgaa cggctccaac gccgccgcac 75780actccgccac ccgacccgcg aacaccggcg acgaacccaa caactccacc gccataccca 75840cccactgcga accctgaccc gggaacacga aggccacatc acccgcaccc tcaccggcgg 75900tgccctcgac cacacgcacg gacggctcac ccgccgccag cgcgtccagc cccgcgagca 75960gctcctcacg gtcggacccc acgaccaccg cacgccggtc catcgccaca cgcgacgaca 76020ccagcgcatg cgccacgtcc agcaccgacg cgtcggacgc acgcaccgac gccgccagac 76080gcgccgcctg ctcccccacc gccgcacgcg acttacccga caccatccac gaccggacgg 76140tgtcggccga gtcgtccgcg gagggctcgg tgccgggctc ctccgcgcgg ggggtctggt 76200cctcgggggc ctgttccagg atgaggtggg cgttggtgcc gctgacgccg aaggaggaca 76260cggcggcgcg gcggggccgg ccggtctcgg gccaggtggt ggactcggtg agcagttcca 76320cgcccccggt cgcccagtcg gcgtgcgggg tgggcgcgtc gacgtggagg ctcttgggca 76380gggtgccgtg gcgcatggcc tgcaccatct tgatgacccc ggcgacgccg gaagcggcct 76440gggtgtggcc ggtgttggac ttcagcgagc cgagcagcag cgggtgcgcg tcaccgcggt 76500cctgcccgta cgtgttgagg agggcctgcg cctcgatggg gtcaccgagg gtggtgccgg 76560tgccgtgcgc ctcgaccacg tcgacctcgc ccgaggtgag accggcgtcg atgagggcct 76620ggcggatcag ccgctgctgg gaggggccgt tgggggcggt caggccgttg gacgcgccgt 76680cctggttgat ggcggaaccc cggatgacgg cgaggatctc gtggccgttg cgctgcgcgt 76740cggagagccg ctccagcagg atcaggccga cgccctcgga ccaggcggtg ccgtcggcgc 76800cggcggcgaa gggcttgcag cgcccgtcgg ccgccaggcc ctgctggcgg gagaactcgg 76860tgaacatctt gggcgtcgac atgacggtgg cgccgccggc cagggccagc gagcattcgc 76920cctgtcgcag cgaacggacg gccaggtgca gggcgacgag cgaggaggag caggcggtgt 76980ccaccgtcac ggcgggcccc tcgaagccgt acgtgtacga caggcggccg gaggcgacgg 77040cgaccgtgtt gccggtgagc aggtgcccgg cgacctcttt ctggcgcccg cgcgaggcgc 77100cgtcgtagtc gccgtgcccg gtgccgacga agacaccggt ggtgctgccc gcgagggtgt 77160cgggcaggat gccggcgcgc tccagcgcct cccaggaggt ctccagcagg agccgctgct 77220gcgggtccat ggccagggcc tcgcgcggcg agatcccgaa gaacgccggg tcgaactcac 77280cggcgtcgtg gaggaatccg ccttcacgcg tgtaggtggt gccgtggtgg gcggggtccg 77340ggtggaacag gtggtcgagg tcccagccgc ggttcgcggg gaacgcggag atggcgtcca 77400cgccgtcgcg ggccagttcc cacaggtcct ggggggtgcg cacgccgccg gggtagcggc 77460aggccatggc gacgacggcg acgggctcgc ggtcgcggtt ctcggcgtcg ctgagctgct 77520gccgggcctc ctggagatcg gtgacggccc gcttgagata tgcgcggagc ctgtcctcgt 77580tcgacatcag gtgcccacct ctccatgaga taccggaaaa cgcggaggcg gcgattcccg 77640aattccacgc cccgacccag ttcgcgctcc cggaagtcag gggacccgtc cgggaaagcg 77700gccacgggcc gtacgggacg gcccggtgcg agagccagtc tggccgtctt ggactgcggg 77760tttccctaaa gcatggggag gctggtgggg acgcgcgttc cggtcgaggc gggttctccg 77820tccgaggtct ggtgaattcc ctcgtcggtt tccgatatcg gcggggcgga tcgccgggcg 77880gggaattcgc gacggcggag ggcggaacgg ctcggtcgtg gagccgacga ggccccgctc 77940cgtcgaaggt gacggaacgg ggcctcgctg ggtggggggc agtcagccgg cgggggcggt 78000ctccggcgcg tcggtggtgg ccgtggtctc cagggcggtc agccacgcgt ccatgaggtc 78060ggcggcgggg ccggagtcct cacggatgag ggagaggtgg tcggagtcca gggggcggac 78120ctccgcgtcg gggacgaccg gaaggtgcag gcgctcgggg tcgggggcgg ggacgccctc 78180
gatgagcgcc cgggtgcact tgatctccag gacggggacg gtggtggggt gcacgtcgag 78240ggcgttgagg agcaccatcc agcggcccat ggcggacagg cgggagttgg tcaggcgcac 78300cggggagtcg tcgacggcgg tgaagttgaa cttcatcatg ccgttgtagt cgacgccctc 78360gtcgctcttg tgctggatgc tgagggtgtc gaggaggacg acggcctcgg gccggatgcc 78420ccaggtgtgt tccaggactc cggccgccgc gtaggcgagg gagccgccgg aggagtggcc 78480gaccaggacg aagggcttgc cgtcggcggc ctggagggtg cagtcggcga tgatccgcgc 78540ggccgcctcg gcgtgggcgg gcagggcctc gccggtgttg aagccgatga ggggcagcgc 78600ggtgacgtcg cggcggcccc ggaagtggcc ggagagagtg gcgtactggt ggacgccgcc 78660gttggcggtg ggggcgctga cgcacaccag gcgggtcggt gcggggccct cggcgagcgt 78720caccggccag ggcaggtcct ccagttcggc ggtcacctcg aaggaggggc gcagcgccgc 78780gacggtggcc agcatgcgct gtgcctcgcg caccttgccc tgggccaggc cgtcgaggaa 78840cagccgctcc atggagtcgt tctcggtggg gccgccggcg gcgccggtct gcggggcgcc 78900gctgccctgg gcgccgagtt cggtgcgcag ccaggcggtg agcccgttcg gggagccgct 78960gtcgtagacg atgctcgcgg ggagctggag accggtgatc tcgctgagct tgcggcgcag 79020ttcgataccg atcagggagt cgaagccgag ctccaggaag tcgcggtcgg ggtcgatctc 79080cgaggtgtcg gtgtggccga ggagtgccgc ggcgcaggcc acgaccgtct cgcgcagggt 79140ggtctcctgc tcccccgcgc cgaggttcgc gaagttctcg cggaaggagg ccgcgtccag 79200gacgcgggcc ggggcgtcgg tcgtggcggc ggcggtccgg cgcgggggac ggaccaggcc 79260ccgcatgagc ggcggcacct cgtcgaggtg ggcggtggcg ggcggtgcgg ccaggttggt 79320ggcgaccacc aggggttcgc cgagcgtgag cgcggtgtcg agaaggtcca gggcgcggtc 79380gccgggcagg gtgtcgatgc cgccggagcg gatgcggcgg cggccggtgt cggtgaggag 79440gtcgccggcc atgccctcgt cgaggtccca ggcgccccag gccacggaga gggccggcag 79500gccctgggcg gcccggtgct gggcgagggt gtcgaggaag aggttggcgg cgacgtagtt 79560ggcctggccg gcgcgggcgg tgacgccgga gatggaggag tagaggacga aggcgtcgag 79620gccgaggccg cgggtgaggt cgtgcaggtg ccaggcggcg tccgccttgg accgcaggac 79680ggtggccagg cggtccggtg tgagggaggt gacgacggcg tcgtcgagga ctccggcggc 79740gtgcacgacg gcggtgagcg ggtgctcggc ggggatgccg ccgagcagtg cggccagggc 79800gtcgcggtcg gagacgtcgc aggcggcgac ggtgacatgg gcgccgaggg cggtcagttc 79860ctcgcgcagg gcggcggcgc cgggggcgtc ggggccccgg cggcccgcca gcaggagccg 79920ggtgacgccg tggcgggtga cgaggtggcg ggcggtgcgg gcgccgaggg tgccggtgcc 79980tccggtcacc aggacggtgc cgtcacgggt gacaccgaac gggtcggtgt tctccgggcc 80040gtcctggggc gtgggcgggg gcagttgggt gaggcggccg acgaggacct gtccgtcgcg 80100gacggcggcc tgcggctcgt cgacggcgag gacggcctcc agggggaacg gggcgcccgg 80160ttcgacgtcg accagggtga agtcggcggg gtgctcggcc tgggcggtgc ggaccatgcc 80220ccacaccgcg gccccggcca ggttggcggc cgggtcgccg ggacgggcgg ccacgacgcc 80280gcgtgcgacg aagaccagcc gggcgcccgc cgggcggggg ccgtccagcc actcctggag 80340gagggcgagg gtggcgcggg tcgcctcgtg ggtggcggtg accgggtcct cgcctccgtc 80400ggggacgggt acgaggacct gggtgcccgt ctcggtgacc tcgtcgagcc cggccaggac 80460cgtaccggtg ccgaacgggt cggtgccgag ggcgacccgg gggcctccgg cggcgggccc 80520ggtgggttcg gcggggaccc agtcgaggcc gaggagggtg gagcggtcgg ttccgggctt 80580gagcaggcgg gccgccccgg agggccgcag ggccagcgac tcgaccgaca gcacgggccg 80640gccggcggtg tcgacggcgg cgatgctgac ggtgtcctca ccggccttgg tgatacggac 80700ccggagccgg gcggccccgg aggcgtgcag ggtgacgccg gaccaggaga acggcaggag 80760tccctggtcg gcgtccttca gctcgacgta ctccacggcg tggagggcgg cggtgagcag 80820
ggcggggtgg agaccgaaga gggcggcgtc gtcggcgcgg gcgggcagtt cgagttcggc 80880gaggacctcg tcgccccggg tccacacggc acgcagcccc tggaagagcg ggccgtactc 80940ggtgcgctcg tagaagccgt ccaggtcgac gggggtggcg tcggcggggg gccaggcgct 81000ctggccgaag gcgacctggc gtgctccggc gaccaggacg ccggtggcgt gctgggtcca 81060gggctcgtcg tcggcggcgt cctcgggccg ggagtggatg gcgagcgggc ggcagccggc 81120cgcgtcgggg ggaccgacgc cgacctgtac ggaggcggcg cctcgcgggt cgagggcgag 81180cggggtggtg agggtcagtt cctggacgcg gccgcagccg gcctggtcgc ccgcgcggac 81240ggcgagttcg aggaaggccg tggcggggaa gaggacgagg ccgcccatga cgtggtcggc 81300gagccaggga tgggtgcgca ggcccacgcg cccggtcagc aggacgccct cggaaccggc 81360gagggagacc gcggcaccga gcagggggtg ctcggccggg gtgagcccgg ccccggggat 81420gtcggcggcg tgggcgagcg ggcgcggcca gtagcggcgg tgctgccagg cgtacgtggg 81480caggtcggtg cggcgggcgc cggtaccggt gaaccaggcg gcccagtcga cgcggacacc 81540cgcgacgtgc agctgggcga gaccggtgag cagggcctcc tgctcggggc ggcgggagcg 81600cagcgccggg gctgccacgg cgccggtacc gccgtcgagc gtgtcgaggc tctgccgggc 81660gagggcggtc aggaccccgc cggggccgat ctccaggaag gcgtcggctc ccgcctcggc 81720gagggtacgg acgccgtcgg cgaagcggac ggtctcgcgg acgtgccgca cccagtactc 81780ggggtcggtg agctgaccgg gcgcggcgag ggcgccggtg acgttggaga ccaccgggac 81840gcgcggctcg gcgtactcca gcgagcgggc gacctcgcgg aaggcgtcga gcatcggctc 81900catcagcgcc gagtggaagg cgtggctgac ggacagacgc tggatcttcc gcccttcggt 81960ctcgaactcg gcggcgaggg ccgtcacttc accttcggga ccggacacga cgacggagtc 82020gggaccgttc acggcggcca gggacaagcc ctcggtcagc cggggcgtca cctcggcctc 82080cgtcgcccgt acggccacca tcgccccgcc cgtggggagt tcctgcatca ggcgggcgcg 82140ggcggcgacc agccggcagg cgtcgtcgag cgagaagacc cccgccacgt gcgccgccgc 82200gatctcaccg accgaatgcc ccgccacgaa ctccggcgag acaccccacg actccaccag 82260ccggaacagc gccacctcca ccgcgaacag cgccggctgc gtgtacccgg tcacgtccag 82320cagcgccgcc gcctcggagc cctcctccac gaacagcacc tcccgcagcg aacgctccag 82380ccccgtgtcc agacgggcca ccaccgcgtc aagcgcctcc gcgaacaccg ggaaacgctc 82440gtacaactcc cggcccatcc ccgcacgctg cgaaccctgg cccgagaaga ggagagcgag 82500tgtgcgctcg gtggcccgtc cccgcgcggc ctcggccggt tcgccctcgg ggcccgcgag 82560gagcaccgca cggtgttcga aggtggagcg tccggtggcc agggagtgac cgatgtcgag 82620cggcgcggcg ccggtgaccg aggtgacgcg ggcgagctgc gcgtcgagcg cctcgcgggt 82680cttcgcggac accggccagg ggacgagggt gggcctggcg gtgggcgtcc cgtcctcggg 82740cgtcgcgggc tcctcgggct cgggcgcctg ctcgatcacg acgtgggcgt tggtgccgct 82800gatgccgaag gaggagaccc cggcacggcg ggggcggccg gtctcgggcc aggcggtgtt 82860ctcggtgagc aggcggacgt tgcccgcgcc ccagtcgacg tggctggagg cctcgtcgat 82920gtggagggag cggggaagga cgccgtgctg gatggcgagc acggtcttga tgacgcccgc 82980gacgccggcg gcggcctggg agtgcccgat gttcgacttc accgagccga gcagcaacgg 83040ccggtcctcg ggccggtcct gcccgtacgt cgccagcagc gcctgcgcct cgatcgggtc 83100gcccagcggg gtgcccgtcc cgtgtgcctc cacggcgtcc acgtcggcgg cggagagtcc 83160ggcgcccgcc agggcctggc ggatgacgcg ctgctgggac gggccgttgg gggcggtcag 83220cccgttggag gcaccgtcct ggttgacggc cgagccgcgc accacggcca gtacctggtg 83280gccgttgcgg cgggcgtcgg agagccgttc caggacgagg acgccggcgc cctcggccca 83340gccggtcccg tcggcggagt cggcgaacgc cttgcagcgg ccgctggtgg agatgccgcc 83400ctggcgggtg aagcccgaga agcccatcgg ggtggacatc acggtgacgc cgcccgccag 83460
ggcgaggccg cactcgccgt tgcgcagcgc ctgggccgcc aggtggatgg cgaccagcga 83520ggcggagcag gcggtgtcga cggtgacggc gggcccggcg aggtcgaagg cgtacgacag 83580gcggccggag accacgctgg cggtgaggcc ggtggtggcg tggccctcgg cgtcctcccg 83640ggagttcatg acgacgccga cgtagtcgac gccggccgtg ccgacgtaga cgccggtgcg 83700gctgccgcgc agggagaccg ggtcgatgcc cgccctctcc accgcctccc aggtggtctc 83760cagcaggatc cgctgctgcg ggtccatggc gaccgcctcg cggggcgaga tcccgaagaa 83820gcccgcgtcg aaggcggcgg cgtcgacgaa gccgccctcg cccgccgcgc tgctgccggc 83880gccctcgccg ttcagggcgt cgaggtccca gccccggtcg gcggggaagg gcgcgatgcc 83940gtcacggccc tcctccagga gccgccagta ctcctcgggg gtcgcgacgc ctccgggcag 84000ccggcaggcc atgccgacga ccgcgatggg ttcgcggcgg tgggcctccg cctcctccag 84060ccgccggcgg gtcttgtgca gatcggccgt gacccacttg aggtagtcga cgagcttctt 84120gtcatcgggc atgacgtggg gaaccttcct gtggtggatc aagctcgtct cagaggtcgc 84180ccaggcggcc gagctcgttg tcgatgaagg cgaagacctc gtcggtgctg gccgactcga 84240agtcgtcgtc gagggaggaa ccggcctgct cgggggcgtt gccgcgcacc tggtcgagca 84300tctggcccag tcgcagggcg aggcccgcgc gggtgatctc gtccgggtcc ccggccgaca 84360gcagcgtctc gaagcggtcg agttcggcga ggagcgacgg tccctgcggg acggcgtcgt 84420caccggccag ctcggcgacg aagtgttcgg cgagcgcggt gggggtcggg tagtcgaaga 84480ccagcgtggc gggcagccgc agcccggtcg cggtcgccag gtggttacgg agttccagcg 84540ccgtcagcga gtcgacgccc agctcgcgga actcctggct gacgtcgacg gacttcggtg 84600aggcgtggcc caggacccgg gcggcctgtt cgcggaccag gtcggtgacg taccggacgc 84660gctcggcggc accgagcccg gcgagcttgg cgccgagcgc ggccgaagcg ccggccgcgt 84720ccgtggaggc ggcggtacgg cggccgctgc ggaccatgga gcggaacagc gggggcaggt 84780cctccggcgc cgcgcccctc atcgctccgg cggcgagacc gaccaggacg aggaaggact 84840cgtccgagcc ccgggcgatg tcgaacagtt ccaggccctg gtcgacggcg agcggcggga 84900gtccgccgga ctgcatgcgg cgcatgtccg tgtcggacag ggtgctggtc atgccgccgt 84960cgtgggccca ggggccccag gccagcgagg tgccggccag gccgaggtgg cgccggtgcc 85020gggtgagggc gtccaggaag gagttggccg cggcgtagtt gccctggccg gggctgccca 85080tgacgcccgc gacggacgag aagggcagga aggcggcgag gtcgaggtcg cgggtcagct 85140cgtgcaggtg ccaggcggcg tccaccttgg gccgcaggac ggcggcgagg cgttcgggcg 85200tgagggaggc gatcaccccg tcgtcgagga tgcccgcggt gtgcacgacg gcggtgagcg 85260ggtgtgcggc cgggaccgcg tcgaggagcg cggccgtggc ctcgcggtcg gcggtgtcgc 85320aggcggcgac ggtgacctcg gcgcccagtt cccgcagctc ggcggcgagt tcggcggcgc 85380cgggggcgtc cgcgccgcgc cggctggtca gcagcaggtg gcgcatgccg gcccgggcga 85440agcggcgggc gaggtggccg ccgagtgcgc cggtgccgcc ggtgatgagg acggtgccct 85500cggggttcca gcggggcggc atggtcagca cgatcttgcc ggtgtgcttg gcctggctca 85560tgaagcggaa cgcctcgcgg gcccgccgga cgtcccaggt gcgcaccggg agcggccgca 85620gcgcgccctc ggcgaagagg tcgaggagtt cgccgaggag ggccttgttc cgctccggtc 85680cggcctcgcc gaggtcgaag gcctggtagc ggacctcgcc cgtggtctgc gggtcgcgga 85740tgtcggtctt gcccatctcc aggaaccggc caccgggagc cgtgatccgc aacgacgcgt 85800ccacgaagtc accggcgagc gcgttcagga ccacgtccat ccccgcgcca ccggtgacct 85860cccggaactt ctcctcgaag cccaggtcac gcgaggacgc gatgtgagcg tcgtcgagcc 85920ccagactccg cagcgtctcc cacttgggct cgctcgccgt ggcgaagacc tcggcgccca 85980cgtgccgcgc gagctggatc gccgccatac cgacaccacc cgcaccggcg tggatcagca 86040cccgctcccc cgcctggacc gaggccaggt cacggaaggc gtacagggcg gtgaggaaga 86100
ccagcggcac cgaggccgcg tcccgcatgg tccagccttc ggggacctgg gtgacgtagt 86160gctggtcggc gacgacgacg gggccgaatc cgccgggcac ggtgccgagg acgcggtcgc 86220cgggggcgag gccggtgacg gcctcgccga cggcggtgac cacgccggcg gcctcggtgc 86280ccatggggcc ggactcgccc gggtacatgc cgagcgcgtt cagcacgtcg cggaagttga 86340ggccggccgc ctcgacgtcg atgcggacct cgtgggcgcc gagtggctgg aggacctccg 86400ggcacggggc gaggaccagg ttgtccaggc tgcccttgcc ggtggtgtcc aggcgccacg 86460gggtgccggt gggggcgagg aggccgggtg ccgtggtgag ccgttcgagc cggccgacca 86520gcgcggtgcc ctcgcggacg gcgaactggg cgtcgccgct gcccagcagc cccggcaggt 86580cgggcaggag cgcgcgcagg gtgtccgggg cgtccgctcc gtcgaggtcc agcaggacga 86640agcggtccgg gttctcggac tgggcggagc gcaccaggcc ccaggcggca ccggccgcga 86700ggtcgcggac ggtctcggtg tcgtgggcgg cgaccgctcc cgtggtgacg aagaccaggc 86760gggagtggcc gagccgctcg gcggcctgcc actgctggag caggtcgagc gtccgcgtgg 86820tcagcgcgtg gacggccgcc ggcacgtcgg cgccgtgggc gtccgggtcc ccggcccccg 86880ccgcgtcacc ggcaccggcg agcgggacga ggacgaagtc gggtgctgcg gacgggtcgg 86940cgtcggggcc ggtcagctcg gtgagggagg ccagggcggt gcccacgccg aagacgtcct 87000cgccgagggt cgccgcggtc aggccctcgg tgggcttgac cgcgggtgcg gccgtccagt 87060ccaggcggaa gaccggctcc tggccaccgg tggcgaccgg ggcgctgggc accgtggggg 87120cgcgcaggac cagggactcg gcgcagagca ccgggacgcc ctccgcgtcg acggcggtca 87180gccggaccga ctcgtccccg agccgggtga tccgcacccg cagcgtggtg gcgcccctgg 87240cgtgcaggga gacgccgttc caggcgaagg gcagcagcgg gttctcctcg ctgcccaccc 87300cgaggtagcc gttggcgtgc tggacggcgt ccaggagcgc ggggtgcatg ccgaagtact 87360gggcgtcccc ggcgacctcc tcggggaggg cgacctccac gaaggcctcc tcaccgcgga 87420gccacacctg gcgcaggccc tggaaggacg gcccgtaccc ggtacggctg tagtcgccgt 87480cgaactccac ggagaccgcg tccgccgggg gccagacggg ggcttcgaag gagtcggtgc 87540gctcctgaac ggccaggaac ccgtcggcgt gccgggacca cggcgcgtcg acggcgtcgg 87600agggacggga gtagaaggtg accggcctgg tgccggtgtc gtcgggagcg ccgacgacga 87660cctggacgga gaccgggtgc ttctcgtcga ggaccatcgg cgcggcgagg acgagttcct 87720cgacgcgggc gcagccgacc tggtcggctg cgcggatggc catctccagg tagccggtgc 87780ccggcatgat gacggtgccg ccgacacggt ggtcgaggag ccaggggtgg acctgcatgg 87840agatctggct ggtgaacagg acgccctcgg agtcggcgag cgggacggcc gcgccgagca 87900gggggtgttc ggcggagatg aggccggccg tgctgatgtc gccggtgagg gcggccgggc 87960gcggccagta ccgctcgcgc tggaaggcgt aggtgggcag ctcggtgcgg cgggcgccgg 88020tgccggtgaa ccaggcggac cagtccaccc ggacgccgct gacgtggagc ccggcgaccg 88080cggtcagcag cgcggtctcc tcgtcgcggt ccttgcgcag ggccgggagg acgaccgcct 88140cgtcccggtc gtcggcttcg aggacgctct gggccagggc ggtcaggaca ccgccgggtc 88200cgatctccag gaaggcgttg gcgccggcgc cggccagggc gcggacaccg tcggcgaagc 88260ggacggtggc gcggacgtgc tcgacccagt acgcggcccg ggtgagctgt ccgggttcgg 88320cgagggcgcc ggtgacgtcg gagaccaccg ggatacgcgg ctccgcgtac tccaggctct 88380ccgccacctg ccggaacgcg tcgagcatcg gctccatgag cggggagtgg aaggcgtggc 88440tgaccggcag gcgctgcgtc ttgcggccct cggccgcgaa cgcctccgcc agggcgaggg 88500cttcgtcctc ggtgccggcg atcaccacgg agtcggggcc gttcacggcc gccagggaca 88560ggccctcggt gagccggggc acgacctcgg cctcggtcgc ccgcacggcc accatcgccc 88620cgcccgaggg cagttcctgc atcagacggg cacgggcggc gaccagccgg caggcgtcct 88680ccagcgagaa gactcccgcc acgtgcgccg ccgcgatctc gccgaccgag tggcccgcga 88740
cgtggccggg gcgcacgccc cacgactcga cgaggcggta cagggcgacc tcgacggcga 88800acagcgcggg ctgcgtgtac ccggtcgtgt ccaggagtgc cgccgtcccg gagccctcct 88860ccgcgaacag cacctcccgc agcgaggggg tcaggccggt gtccagccgg gcgaggacgg 88920cgtccagcgc ctcggcgaag accgggaaac ggtcgtacag ttcgcggccc atgccggcac 88980gctgcgatcc ctggccggag aagagcaggg cgagcgagcg caccgcggcc cggccccggg 89040cggcctccca cggcgtcgtg ccggggcgcg cgaggagcac ggcgcggtgg tcgaagcggg 89100cgcggccggt ggccagggag tggccgacgt cgagcggggc cgcgccgtcc agggcggtga 89160cggccgcgat ctgcccgtcg agggcctcct cggacttggc cgacaccggc cacgggacga 89220cggtgggtgt gggccccggc aggagggccg caccgggctc ggcgggctcc ggggccgggg 89280cctgctcgat gatggtgtgc gcgttggtgc cgctgatgcc gaaggacgag atgcccgccc 89340gccagggccg gccgctgtcg ggccaggccg actcctccgt gagcagccgt atcgccccgg 89400actcccagtc gacgtgggtc gagggccggt cgacgtggag ggtgcgcggc aggacgccgt 89460ggcgcatcgc catgacggtc ttgatgatgc cggcgacgcc ggcggcggcc tgggtgtggc 89520cgatgttcga cttgatggag ccgagcagca gtggccggtc cgcgggccgg tcctgcccgt 89580acgtcgccag cagggcctgg gcctcgatcg ggtcgcccag cggggtgccc gtgccgtggc 89640cctcgaccgc gtcgatgtcg gagggggtca ggccgccgcc ggccagcgcc tggcggatga 89700cgcgctgctg ggaggggccg ttgggggcgg tgaggccgtt ggaggcgccg tcctggttga 89760tggccgagcc gcgcacgacg gcgaggacct cgtgaccgtt gcggcgggcg tcggagagcc 89820gctcgacgac gatcaggccg accccctccg accagccggt gccgtccgcc gcgtccgcgt 89880acgccttgca gcggccgtcg acggccaggc cgttctggag ggtgaagccg gagaaggcgg 89940acggggtgct catcatggtg atgccgcccg ccacggcgag gtcgcactca cccgcgcgca 90000gcgcctgcat cgcccagtgg agggcgacca gtgaggagga gcaggcggtg tcgacggtga 90060ccgccgggcc ttccaggccg aaggtgtagg ccagccggcc cgagacgacg ctggcggcga 90120ggcccgttcc ggcgtggccc tccaggtcct cgcgggagtt catgacgagc gtggagtagt 90180cctggccgtt ggtgccgacg aagacaccgg tacggctgcc gcgcagttgg tcggcgtcga 90240tgccggaccg ttcgaacgcc tcccaggtgg tctccaggag caggcgctgc tgcgggtcca 90300tcgccagcgc ctcacgcggg gagatgccga agaaggccgc gtcgaagtcg gctgccccgg 90360agaggaatcc gccgcgcagc gtggcgctgc ggccccggcc gtcggccccg ccggtcgtca 90420gggtggcgag gtcccagcct cggtcctcgg ggaatgcctc gatgccgtcg cggccctcgc 90480tgaccatggc ccacaggtcc tgcggggagc ggaccccgcc gggcaggcgg caggccatgc 90540cgacgatgac gaccgggtcc tcggccagtg ccgccggggt ggcccggccg gagcccgggg 90600tgtcctgctc gcccagcagt tcggcgagga ggtgttcggc gagggcctgg gcggtcgggt 90660agtcgtagac ggtggtggcg ggcaggcgca ggccggtcgc gagggcgatc cggttgggca 90720gctcgatggc ggtgagcgag tcgaagccca ggtcgcggaa gttccggtcg gcggcgacgg 90780cctccggtcc tgggtggccg aggacggctg ccgcgtgggt gcgcaccagt tccgtgacgc 90840tgtcggtgcg ttcgtcggcg gggaggccgc gcagccgctc ggcgagtccg gaggcggccg 90900tgcgggcacg gtcgcgggtg gtctcggcgt cggcgagggc ctgccgggcg tcgggcaggt 90960cgcgcaggag cgcgttgccg cgcatgccga tcaggccgtc caggacctgc ggctggcgcg 91020ggtcgaacag gacgggtgcg gcctcgggcc gggtcacggc ctgccgcagt gcggccaggg 91080ccagatccgg gtggaccgcc gggtgcccgg ctcctgccct cggggcggcg ggggcgtcgt 91140cccctccggt ctcgtcggcg tccccgatcc aggcgcccca ggccatcgag gtggcgggca 91200gcccctcact ccggcggctc cgggcgaggg cgtcgagtac ggcggtggcg gcggcgaggt 91260tggcccggcc cgccgtgccg acggtcccgg cgacggagga gcagagggcg aagacgtcga 91320ggtcaaggcc gcgggtcagc tcgtccaggt ggagggcggg cgcggtccgc gcgtggtgcg 91380
gagcggcgaa tcgttccggg gtcaggtcgt cgaggactcc gtcgtcgacg actgcggcgg 91440cgtggacgac ggcggagagc ggggtgtcgg cggggatgcc gtcgaggacg gccgcgaggg 91500cgtcccggtc ggcggcgtcg caggcgacga gggtgacgcg ggcaccggcc gcctccagtt 91560cggcgcggag cgcgtcggcg ccgggggcgt cgggaccgcg tcggccgagg agcaccaggt 91620gcgcggcgcc ggctccggcg agccagcgcg cgacgtgggc gccgcgtccg ccggtgccgc 91680cggtgacgag gacggttccg cggggctgcc agccgtcgcc gggctccgcg gccggtgccg 91740ggacgagccg gcgtgcgaag gcggcggagg agcggacggc cacctggtcc tcgccgtcat 91800gtccggcgag gacggcggcg aagcggcggg cggcgcgttc gtcccacgcg gagggcaggt 91860cgacgagccc gccccacacg gcggggtgtt cgagggcggc gacccggccc aggccccaga 91920cggcggcctg ggcgggggcg gtgacggcct cggaacccgc caccgcgacg gctccccggg 91980tcaggcacca caggggggcg tcgaccccgg cgtcgagcag ggcctggaac aggacggcgg 92040tcggggcggc gggggctccc gccgggtcgc cggagacggc tgcctccggc gtgtcggcga 92100gggccagcag ggagaccaca ccggcgaggg cggcgccgtc ggcggagagg gcgcgcaggc 92160ggtcggcgag cgcgtggcgc tcgacggtgt ccacggtgag gcggacgacg tcggtgccga 92220cggcgtcgag gacggcggcg gtccaggggt cctcgccgta cgcggcgggc acgacggcca 92280gccaggttcc ggccggggcg ggcgcaccgg gcggcagcga cagcggcttc cacgtctcgt 92340ggtggcggtt ggagtcgacc atggcctggt cccggcgccg gcgccgccag gtggagaggg 92400ccgggagtac cgcgccgagc gcctcggtgt ccacggccag gtcggtggcg agacgggtca 92460ggtcctcgcc ctcgacggcg gtccagaagg cgccgtcgag cgggtcggag gagccgccgc 92520ccgtaccggg gcgccgggtg tcgggccagt ggcggacgcg ctggaaggcg taggtgggca 92580ggtcgacgcg gcgggcgccg gtgccctcga agcaggcggc ccagtcgacg gggacgccgg 92640tgacgtggag cgtggcgagc gcggtgagca gggcgggctc ctcggcccgg tccttgcgca 92700gcgcgggcgc ggtgacggcc tcggagtcgg tgcccagggt ctgccgggcg agggcggtga 92760ggacgccgcc ggggccggcc tccaggtagg cgtcggctcc cgcctcggcg agggcgcgga 92820cgccgtcggc gaagcggacg gtctcacgga cgtgccgcac ccagtactcg gggtcggtga 92880gctgaccggg cgcggcgagg gcgccggtga cgttggagac gacggggaga cgcggttcgg 92940cgtacgccag cgagcgggcg acctcgcgga acgcgtcgag catcggctcc gtgagcgcgg 93000agtggaaggc gtggctgacc tgcaggcgct gggtcttgcg tccctcggcc acgaactcgg 93060cggccagcgc ggtcacttcg ctctccgggc cggacacgac cacggagtcc gggccgttga 93120ccgcggcgag ggagagtccc tcggtcagcc gtggggcgat ctccgcctcg gtggcctgca 93180cggccgccat ggcgccgccg gcgggcagtt cctgcatcag gcgggcgcgg gcggcgacca 93240gccggcaggc gtcctccagg gagaagaccc cggccacgtg cgcggcggcg atctcgccga 93300cggagtgacc ggccacgtac tccggcgaca cgccctgcga ctccagcagg cggtgcaggg 93360ccacctccac ggcgaagagc gcgggctggg tgtagccggt ggtgtccagg agagcggccg 93420tctcggagcc ctcctccgcg aagagcacct cacgcagcgg acggtccagc tccgggtcga 93480gcagggcggt gatctcgtcg agtgccttcg cgaacaccgg gtaacgctcg tacagttcac 93540ggcccattcc ggcccgctgg gaaccctggc ccgggaacag caccgcgagc ctgcgctcca 93600cggcacggcc ccgcgccacc tccaccggct cgtcctcggc accggtgccc gccagcagga 93660ccgcgcggta ctcgaaggag gcgcggccgg tggccaggga gtggccgatg tcgagcgggg 93720tggcgccggt gacggtggcg atccgggcga gctgtgcgtc gagcgcctcc ggggacttcg 93780cggagaccgg ccaggggacg gccgccggag ccggggcgga cgcggcggcc ggcgtggcct 93840ccacgatctc cggggcctgc tcgatgatgg tgtgcgcgtt ggtgccgctg atgccgaagg 93900aggagacggc ggcgcggcgt ggacggccgg tctcgggcca ctccgtggtc tcggtcagca 93960ggcggacgtc gccctcggtc cagtccacgt gcgtggaggg ccggtcgatg tggagcgagc 94020
ggggcaggac accgtgccgg atcgccatga cggtcttgat gaccccggcg acaccggcgg 94080cggcctgcgc gtggccgatg ttcgacttga tggagccgag cagcagcggc cggtcgccgg 94140gccggtcctg cccatacgtc gccagcagcg cctgggcctc gatcgggtca cccagggtgg 94200taccggtgcc gtgtgcctcc acggcgtcga cctcgtcggt cgagagcccg gcgctcgcga 94260gggcctgacg gatgacccgc tgctgcgagg gtccgttggg cgcggtcagg ccgttggagg 94320cgccgtcctg gttgacggcg gagccgcgca gtaccgcgag gaccgggtgg ccgttgcggc 94380gggcgtcgga gaggcgctcg acgacgagca tgcccacgcc ctcggaccag ccggtgccgt 94440ccgccgcgtc cgcgaacgcc ttgcagcggc cgtcggtggc caggccgccc tgccgggtga 94500agcccgcgta ccccatggcg gtggacatga cggtgacgcc gccggcgagc gccaggtcgg 94560cttcgccgga gcgcagcgcg tgcgcggccc agtggagggc gacgagcgag gaggagcagg 94620cggtgtcgat ggtgaccgcc gggccctcca gtccgaaggt gtaggacagc cggccggaga 94680tgacactggt ggcgaggccg gtgggggcgt ggccctcggc gtcctgacgg gagttcatga 94740ccagggtggt gtagtcctgg ccactggtgc cgacgaaaac accggtacgg ctgccgcgca 94800cctcggtggg ggtgatgccg gcgcgctcga acgcctccca ggtggtctcc aggagcaggc 94860gctgctgggg gtccatcgcc agcgcctcgc gcggggagat gccgaagaag ccggcgtcga 94920agtcggcgac gtcgtagagg aatccgcctt cggaggtggc gctgcggccg tggccgctct 94980cgccgccgcg ctggagggtg tcgaggtccc agccccggtc ggtcgggaag gcggagatgc 95040cctcctggcc gtcgaggacg agctgccaca gctcctcggg agagctgacg ccgcccggca 95100tccggcaggc catgccgacg atgacgacgg ggtcctcggc gagggccgcg gcgacgggcg 95160tcggggcggt ggcgccagtg gcgcgctgct cgtcgagcag ggcggcgatc aggtgctccg 95220ccagcacgcc gggcgtgggg tggtcgaaga cgagggtggc gggcagccgc aggccggtgg 95280cgccggtgag gcggttgcgc agttcgacgg cggtgaggga gtcgaagccc aggtcgtgga 95340agtcgcgttc ggcgtcgatg gcctgggcgg aggagtggcc gaggaccgag gcggcctcgg 95400cccggatcag gttcacggcg aagcggagcc gctcgtcctc gcgcaggtcg aggaggcgcc 95460gggtgagctc gtcggcggta cccgcgccgc cgacggcggt cgcggcggcg cgccgggtgc 95520cgcggaccag gttgcgcagc agcggcggca cctccccggg catccgggtg gagcccgagg 95580ctccgagggg gacgaggtgc ggctcgtcgg agacgacggc ggcgtcgagg agggcgagcc 95640cctgctcgac ggtgagggcg ggggccgccg acgccgacat gcgccgcacg cccgcgtcgg 95700tgagggtgcc ggtcatgccg ctggtctgtt cccaggcccc ccaggccagg gagagcccgg 95760ccagaccctc ggcccggcgg tactgcgcca acgcgtccat gaacacattg gcggcggcat 95820agttgccctg ccccgcactg cccatcacac cggacacgga cgagaacatc acgaaccctg 95880cgagacccat gtcccgcgtc aactcgtgca ggtgccacgc agcgtccacc ttcggacgca 95940acacccccga caaccgctcc ggcgacaacc ccgtcaccac accgtcgtcc agcacacccg 96000ccgtatgcac cacagccgtc aacggatgcc ccaccggcac cgacgccaac agccccgcca 96060cggcagcacg gtccgccaca tcacacgcca ccacgtccac gtgggcaccc agcccggcca 96120actcctcacg gaaagcgtcc acaccctccg ccgccggacc acgccgcgac accagcagca 96180gacgccgcac cccacgcgcc gacaccagat gacgcgccac ctcacgcccc agaccacccg 96240tcccaccagt gaccaggacc gtgccctcgg ggttccagcg cggcggcatc gtcagcacga 96300tcttgcccac atgcctcgcc tggctcatga aacggaacgc ctcacgcgcc cgacgcacat 96360cccacgcacg caccggcaac gggcgcaacg ccccctccgc gaacaggccg aggagttcgc 96420tcagcatctc gccggtccgg tccggtcccg cctcgccgag gtcgaaggcg cggtaccgca 96480cgtcaccgac cgcgtgcggg tcgcggatgt cggtcttgcc catctccagg aaccggccgc 96540cggaagccgt gatccgcaac gacgcgtcca cgaagtcacc ggcgagcgcg ttcaggacca 96600cgtccatgcc cgcgccacca gtctcctccc ggaacttctc ctcgaagccc aggtcacgcg 96660
aggacgcgat gtgagcgtcg tccagcccca gaccccgcag cgtctcccac ttgggctcac 96720tcgccgtcgc gaagacctcg gcacccatgt gccgcgcgag ctggatcgcc gccataccga 96780cgccacccgc accggcgtgg atcagcaccc gctcccccgc cgacaggccg gcgagttcgg 96840tgagcgcgta gtaggcggtg aggaagacca ggggcaccga ggcggcgtcc tcgtcgctcc 96900actcctcggg tacgcgggtg aggaagcgtt cgtcgatcag ggtctccgtg gcgaggccgc 96960cggggaccat gcccatgacg cggtcgccga cggagagccc ggtgacctcg gggcccacgg 97020cggtgacgaa cccggccgct tccgcgccga gcagtccggc ctcgccgggg tacatgccga 97080gcgcgttcag cacgtcacgg aagttcaggc cggcggctcg tacctcgacc cggacctggc 97140gctcctgagg ctccgccagc tcctcggggc aggggcgcag ggtgagcgcg tccaggctgc 97200cgcgtgacgg ggagtccagg cgccaggggc cgtcggcggg cggcaggagg gacggggcgg 97260tcacggcccg gtcgagacgg ccgaccagga cggcgccctc gcggacgacg aactgggcgt 97320ctccgcccga gagcggaccg aggagttgcg gcagagcggt ctcgacgggg gtggtgtcgt 97380ccaggtcgag gagggcgaac cgggtggggt tctccgactg ggcggcgcgg acgaggcccc 97440agacggcggc ggcggggagg tcgtcgaccc gctcgccggg gcgggcacag accgcgcccc 97500gggtgacgaa gacgaggcgg gtgtcggcga accgttcgtc ggccagccac tcctgcgcca 97560gggcgagggc ctgtgcggcg cgctcgtggg tggcggcggg cacgtcggca ccctcggccg 97620gtgcggggac ctcgaccagg acgaggtcct cctcgccggt cagcggggcg agggtggccg 97680cggccccgcc ggtgccgagg gcaccggcgg tcgtgtgacg ggcggcgggg gcgtcgccgg 97740tctcggggcc ggcggtccac tccaggcgga gcagcgagtc gagttcggtg cggcgggcgg 97800ccggggcgtg gtccccggcg ggaacacgca gcaccagcga ctcggcggag agcaccgggg 97860cgccctcgac gtccacggcg gcgatggaga cggagtcctc gccggtccgg gcgacgcgga 97920agcggaccac ggaggcgccg ccggcgtgca gggagactcc ggcccacagg aacgggagca 97980gctggtgctc gtcgtcgagc ccggcgaagc cgacggactg gacggcggcg tcgagcagcg 98040cggggtgcat cccgtagtac tcggcgtcgt cggcctgcgg gggcagggcc gcctcgacga 98100acgcctcgtc gccgcgcttc cagacggcgc ggatggtgcg gaagaccggg ccgtactcgg 98160tgcggtcgta gaagtcctcc aggccgacgg cgaccgcgcc cttggggggc catatctcgg 98220cgacggcggg gagggtgacg gcgccgctgg tcaggacgcc ggtcgcgtgc tgtgtccagg 98280tggcgtccgg aacgtcggcg gaccgggagt acaggctgac cgtgcgggcg ccgctctcgt 98340cgggggcgcc ggcccagacc tggacgacca cggcggtgtc cgcgggcagc agcagcgggg 98400tggcgagggt gaattcctcg acgcggtcgc agccgacctc gtcggcggcg cgtacggcca 98460gttccaggaa gccggtggcg gggaagagca ccatgccccc gccgacggtg tggtcggcga 98520gccaggggtg cgtggtgagg gagaggcgtc cggtgaagag gacgccttcg gaggcggcga 98580ggccggtggc ggcgcccagc agcgggtgcg cggcggggct cagaccggcg ccggtgacgt 98640ccccgccgtg tgcggagggg cgcggccagt accgctcgtg ctggaaggcg taggtgggca 98700gctcgacgcg gcgggcgccg gtgccctcga agcagccggc ccagtcgacg gggacgccgg 98760tgacgtggag cgtggcgagc gcggtgagca gggcgggctc ctcggcccgg tccttgcgca 98820gcgcgggcgc ggtcacgctg tcgctcgcgg tgccggcggc gtcgagggtc tgctgggcga 98880ggacggtgag gacgccgccg gggccgatct ccagataggc gttggcgccg gcctggtcga 98940gggcgtggac ggcgtcggcg aagcggacgg tagagcggac gtgctcgacc cagtactcgg 99000gcctgctgag ctgcccgggc tcggcgaggg tgccggtcac gtcggagacg acagggatgc 99060gcggttcgcc gtaccccagg cccttggcca cgtcgtggaa ggcgtcgagc atcggctcca 99120tgagcgccga gtggaaggcg tggctgaccg ccaggcgctg gaccttgcgt ccttcggcgg 99180cgaactccga cgccagcgcg tcgacctcgt ggtccggacc ggcgatcacc acggagtccg 99240ggccgttgac cgcagccagg gacaggcccc cggtcagtcg gcctgcggtc tccgcctcgg 99300
tcgcctggac ggccaccatg gcgccgccgg cgggcagttc ctgcatcagg cgggcgcggg 99360cggcgaccag cgtgcacgcg tcctccaggg agaggacgcc cgcgatgtgg gcggcggaga 99420tctcgccgac ggagtggccc gcgacgaact ccgggacgac cccgaaggac tcgaccaggc 99480ggtagagggc gacctccacg gcgaagagcg cgggctgcgt gtagccggtg gtgtccagga 99540gagcggccgt ctcggagccc tcctccgcga agagcacctc gcgcagcgga cggtccagct 99600tcgggtcgag cagggccagg acttcgtcca gcgccttcgt gaacaccggg aaacggccgt 99660acagttcccg gcccatcccg gcgcgctgcg aaccctggcc ggagaacagc accgcgagcc 99720tgcgctcgac ggcgcggccc cgcgccacct ccaccggctc gtcctcaccg tcggcgcccg 99780ccagcaggac cacccggtgg tcgaaggagg cgcggccggt ggccagggag tgaccgatgt 99840ccaggggtgc ggcgtcgagt gcggtgatcc gggcgagctg gccgggcagt gcctcctcgg 99900tacgggcgga gacgggccac ggcacgaccc gcgcgagcgc ctcgggctcc gcctggggct 99960cgggggactc cgtgtcggcg gtctccggct ccggctgttc gaggatcacg tgggcgttgg 100020tgccgctgag cccgaaggag gagactccgg cgcgccaggg gcggccggtc tcgggccagc 100080cggtcgtctc ggtgaggagc cggaccgctc cggcctccca gtcgacgtgg gtggagggct 100140ggtcgatgtg gagggaccgc ggcagggcgc cgtggcgcat ggcgagcacc atcttgatca 100200cgcccgcgac gccggcggcg gcctgggtgt ggccgatgtt cgacttgatg gagccgagca 100260gcagcggccg gtcctcgggc cggtcctgcc cgtacgtcgc cagcagcgcc tgggcctcga 100320tcgggtcacc cagggtggta ccggtgccgt gtgcctccac ggcgtcgacc tcgtcggcgg 100380agagcccggc cgtcgccagc gcctggcgga tgacccgctg ctgggacggg ccgttgggcg 100440cggtcaggcc gttggaggca ccgtcctggt tgatggccga gccgcgcacg acggcgagga 100500cgtcgtggcc gttgcggcgg gcgtcggaga ggcgctccag gacgagcatg cccacgccct 100560cggaccagcc ggtcccgtcg gcggcgtcgg cgaacgcctt gcacaggccg tcgccggcca 100620gcccgccctg acgggtgaac ccgaagaagt tcatgggcgt cgacagcacg gtcaccccgc 100680ccgcgagggc gagagagctc tccccgctgc gcagggactg ggcggcgaga tggagcgcga 100740cgagggagga ggagcaggcg gtgtcgacgg tgagggcggg gccctccagt ccgagcgcgt 100800aggagacgcg gccggagatg acactggtgg cgaggcccgt actggcgtgt ccctcgacgt 100860cctcgcggga ggtcatgacg aggttggcgt agtcctggcc ggtggtgccg acgaagatgc 100920cggtggcgct ggagcgcagg cccgtcgggt cgatgccgga ccgctccagg gcctcccagg 100980aggtctccag cagcaggcgc tgctgcgggt ccatcgccat cgcctcacgc ggggagatgc 101040cgaagaaccc cgcgtcgaac ccgcccacgt cgtgcaggaa gccgccgcgt cgcgtggcgc 101100tgacgccgcg gttgtcgccg ccaccgccga agagggcgtc caggtcccag ccgcggtcgg 101160tggggaagcc ggtgatgcgg tcctcgccgt cggcgagcat gcgccacagg tcgtcggggg 101220tctcgatgcc gccgggcatc cggcaggcca tgccgacgat gacgacgggg tcgtcggcga 101280catcggcggc caccaccggc gtgccggtct cgccgtgttc gtcgaggagt tcggcgacca 101340ggtgttcggc gaggccgtgg ggggtggggt agtcgaagac gagggtggcg gtgaggcgca 101400ggccggtgac ggtggtgagg cggttgcgca gttcgacggc ggtcagggag tcgaagccca 101460gctcgcggaa ctcgcgcccg ggttccacgg cccgggccga ggcgtggccc agtacggcgg 101520cggcctcggt gcggaccagt tcgacggcgt gacggacccg gtcctcggcg ggcagcgcca 101580ggaggcggcg ggcgaggtcg gccgcggtgc tcgcgccgcc ggcggccgtg gccgcggagc 101640gacgcgtgcc gcggacgagg ttgcgcagca gcggcgggac caggccgagg acgcgctggt 101700cgcccgacgg ggcgccgatc ggtacgacca ggggctcgtc ggagccggtg gcggcgtcga 101760ggagggctag cccctgctcg acggtgagcg gggcggcccc ggaggcggtg acgcgctgca 101820tgtcggcctc ggtgagggtg ccggtcatgc cgctggtctg ttcccaggca ccccaggcca 101880gggagagccc ggccagaccc tcggcccggc ggtactgcgc caacgcatcc atgaacacat 101940
tggcagcggc atagttcgcc tgccccgcac tgcccatcac accggacacc gacgagaaca 102000tcacgaaccc tgcgagaccc atgtcccgcg tcaactcgtg cagatgccac gcagcgtcca 102060ccttcggacg caacaccccc gacaaccgct ccggcgacaa ccccgtcacc acaccgtcgt 102120ccagcacacc cgccgtatgc accacagccg tcaacggatg ccccaccggc accgacccca 102180acagccccgc cacggcagca cggtccgcca catcacacgc caccacatcc acgtgggcac 102240ccagcccggc caactcctca cggaaagcgt ccacaccctc cgccgccgga ccacgccgcg 102300acaccagcaa cagacgccgc accccacgcg ccgacaccag atgacgcgcc acctcacgcc 102360ccagaccacc cgtcccacca gtgaccagca cggtcccctc gggattccag cgcggcggca 102420tcgtcagcac gatcttgccc acatgcctcg cctgactcat gaaacggaac gcctcacgcg 102480cccgacgcac atcccacgca cgcaccggaa gcggctgcaa cgccccctcc gcgaacaggc 102540cgaggagctc ggtcagcatc tcgccgatac gctcgggggc tgcttcgccg aggtcgaagg 102600cccggtagcg gacctcgccc gtggtctgcg ggtcgcggat gtcggtcttg cccatctcca 102660ggaaccggcc accgggagcc gtgatccgca acgacgcgtc cacaaagtca cccgcgagcg 102720cgttcaggac cacgtccatc cccgcgccac cggtcacctc ccggaacttc tcctcgaagc 102780ccaggtcacg cgaggacgca atgtgagcgt cgtccagccc cagaccccgc agcgtctccc 102840acttgggctc actcgccgtc gcgaagacct cggcacccat gtgccgtgcc agctggatcg 102900ccgccatacc gacaccaccc gcaccggcgt ggatcagcac ccgctccccc gcctggaccg 102960aggccaggtc acggaaggcg tacagggcgg tgaggaagac cagcggcacc gaggccgcgt 103020cctcgtcgct ccactcctcg ggcacgcggg tcacgtaccg ctcgtcgacg aggacggtgt 103080cggcgaggcc gccggggatc atgccggtca cgcggtcgcc gacggcgatc ccggtgacgt 103140ccgggccgac ggccgtgacg aggccgaccg cctccgagcc gagcagtccg gcctcgccgg 103200ggtacatgcc gagcgcgttc agcacgtcac ggaagttcag ccccgcagcc cgcacctcga 103260cccggacggc gcggccctcc ggctcgtcca gtgcctcggg tgcggggacg agggagaggc 103320cgtcgatgct gccggggacg gtggtgtcca ggcgccacgg cagccccggg accggcagta 103380ggctcgcgcc ggtggacagc cgggccaggc gggccaccag gacggcctcg tcgcgtacgg 103440cgaactgcgt gtcgccggcc gccaggaggg gaggaagctg cgccaggagt tcaccggagg 103500gcgcggcggt gcccgcgtcg aggtcgagga gggcgaagcg gccggggttc tcggcctcgg 103560cgctgcggac gagtccgtgc accgcggcgg cggcgagatc gctcacgccg gtgtcggtcc 103620cggtggcgac ggcgccccgg gtgacgaaca cgagacgtcc ggcggcggac gtggccggct 103680ccagccactc ctggatcagt gccagggtgc gcgcggtggc cgcgtggacg gccgtggggg 103740tgtcgtcctc gacgccctcc agcgggacga ggaccaggtc gggggcgggc gtgacggcgg 103800cggcgtcggc gagggaggcg agagggccgg gccaggcgcg gtcggtaccg agggtgacgg 103860cgcgcacggg ggcggcgccg ccctcgggtg cggccaccca ctccaggcgc aggagcccgt 103920cctgctcact gctgtgcagg gtgggggcgc gcagcgccga aggggcgcgc aggatgaggg 103980agtcggcgga gagcaccggg gcgccctcga cgtccacggc ggcgatggac accgtgtcct 104040caccgacccg ggcgacccgc acccgcacca cggaggcgcc gcccgcgtgc agcgagacgc 104100cggaccagga gaacggtacg agcttcttgc cgtcaccgag accggcgaag ccgacggact 104160ggacggcggc gtcgagcagc gcggggtgca tcccgtagta ctcggcgtcg tccacctggg 104220gcggcagcgc gacctcgacg aaggcctcgt cgtcgcgggt ccagacggcg cgcagtccct 104280ggaagaccgg gccgtactcg gtgcgcgcgt agaagccctc caggtcggcg gcgacggcgc 104340cgcggggcgg ccagacggtg gagtcgaagc cggtgacgcg ctcgccacgg gtcagcagac 104400cggaggcgtg ctcggtccag cgctgctcgg ggtcgtcgac ggcctgggag tagacggtga 104460cgcggcgggc gccgtcggcg tcggccgcgc cgacgtggac ctggacgacg gccgccgagt 104520tctcggtgag gatgaggggc ttggcgaggg tgaggtcctc gacgcggtcg cagccgacct 104580
ggtcaccggc gcggacggcg agttcgagga agccggtgcc ggggaaggcc accatcccgc 104640cgaccgtgtg gtccagcagc cacgggtggg tctgcgcgga gaggcgtccg gtgaagagga 104700ccccgtcgct gccggccagc gacacggcgg cgccgagcag cgggtgttcg gcggagacca 104760ggcccgcggc gctgacgtcc ccggcggcgg ccatcaggac gggccagtag cgctcgtgct 104820gccagcggta ggtgggcagt tcggtgcggc gggcaccggt gccctcgaag caggcggccc 104880agtcgatgtc gacgccggcg acgtggagcc gggccagcgc ggtgagcagg gcgcgctcct 104940cggcgcggtc cttgcgcagg acggggacgg cgacgacgtc gggcccgtcg aggcactgct 105000gggcgagggc ggtgaggatg ccgtcggggc ccagttcgac gaaggtgtcg gcgccggcgc 105060cggccagggc gcggacgccg tcggcgaagc ggaccgtgga gcggacgtgg tccacccagt 105120actcggggtc gcacagcagg cggtcgtcgg cgagggcgcc ggtgacgttg gagacgaccg 105180gcaggaccgg ctcgtggtag gtgaggctct cggcgacgcg gcggaactcc gcgagcatcg 105240gctccatcag cggcgagtgg aaggcgtggc tgaccgccag gcgctgcgtc ttgcggccct 105300cggcggcgaa ctcggcggcc agcgcgagga cctgggcctc ctcgcccgcc agggtgacgg 105360agtcggggcc gttgacggcg gcgagggcga cgccctcggt caggcgcggg gcggcctcgg 105420cctcggtggc ctggacggcg accatggcgc cgcccgcggg cagttcctgc atgaggcggg 105480cgcgggcggc gaccagcgtg caggcgtcct ccagcgagaa cacaccggcg acgtgggcgg 105540cggcgatctc gccgatggag tgcccggcca cgaagtccgg gaccacgccc caggactcga 105600cgaggcggtg gagggcgacc tccacggcga agagcgcggg ctgggtgtag ccggtggtgt 105660cgaggagggc cgcctcgggg gtgcggcgcc gggcgaacat cacctcccgc agcgggcggt 105720ccaggcgcgg gtcgagcagg gcggtgatct cgtccagtgc cttcgcgaac accgggaagc 105780gggcgtacag ttcgcggccc atctcggcgc gctgggcgcc ctggccggag aacaggacgg 105840cgagggagcg ttcggtggcg cggccgcggg ccgcctcggc ggggacgccg tcgaggccgg 105900agagcaggac ggcgcggtgc tcgaagaggg agcgcccggt ggccagggag tggccgatgt 105960cgaggggcgt ggcgtcggtg agagcggtga cggcctcgat ctgcgcgtcc agggacgcct 106020cggagcgggc gtgcacgggc cacggcacga cggtcgggac ggtctcgggc tccgccgggg 106080actccggctc ggcggcgggt ccggcctgct cgatcacgac gtgggcgttg gtgccgctga 106140tgccgaagga ggagacgccg gcgcggcgcg ggcggccggt ctcgggccac tcgacggcct 106200cggagagcag ccggacggcc ccggagtccc agtcgacgtg gcgcgagggg tggtcggcgt 106260ggagggagcg cgggacgacg ccgtgccgca tcgccatgat catcttgatg acgccggcga 106320ctcccgcggc ggcctgcgcg tggccgatgt tcgacttgac ggagccgagc agcagcggcc 106380ggtcctcggg gcggccctgg ccgtaggtgg cgagcagggc ctgggcctcg atggggtcgc 106440cgagcggggt gccggtcccg tggccctcga ccgcgtcgac gtcggcggcg gtgagtccgg 106500cggcggccag cgcctgctgg atgacgcgct gctgggaggg gccgttgggg gcggtgaagc 106560cgttggaggc gccgtcctgg ttgacggcgg agccgcgcag cacggcgagg accgggtggc 106620cgttgcggcg ggcgtcggag agccgctcca ggaggaggac accgaccccc tccgaccagc 106680cggtgccgtc ggcctcgtcg gcgaacgcct tgcagcggcc gtcaccggcc aggccgccgg 106740cgcggctgaa ctccatcagc gagccggggg cggacatgac gttgacgccg ccggcgaggg 106800cgaggccgca ctcccccgcg cgcagggcgt gggcggcggc gtggagggcg acgagggagg 106860aggagcaggc ggtgtcgacg gtgaccgccg gcccctccag gcccagggtg taggagaggc 106920ggccggaggt ggcgctggcg gcgatgccgg tgccgatgtc gccggtcgcg tcggcgaggg 106980agcgcaccag gaggtacgcg tagtcctggc cgttggtgcc gacgaagacg ccggtgcggc 107040tgcctcgcag gctgcgcggg tcgatgccgg cgcgctccac ggcctcccag gaggtctcca 107100gcagcagccg ctgctggggg tccatggaga cggcttcgcg cggggagacg ccgaagaagg 107160cggcgtcgaa gtcggcgacc ccgtcgagga agccgccccg ctggctgacg gagtggccac 107220
gggcgtcgac cccggcgtcc cgcagcgcgt ccaggtccca gccccggtcg gtggggaagc 107280cgccgacggc gtcggtgccg gaggcgacga ggtgccacag gtcctcgggg gaggtgactc 107340ccccggggta gcggcagctc atcgcgacga cggcgacggg ctccgaggcg gcggccgcga 107400ggtcgcggtt ctgccggcgc aggcgttcgg tctccttgag cgaggcccgg agagcttcga 107460caactttttc gctgggcgtg gtcatgatgg ctccgtcagt cccgctcttc gtcagggtta 107520ccgttgagtg ccgcctgcac caggccgtcg acgtccatgg cgtcgatcga cgcgtcgtag 107580gcgtcgccgg ccccggaggg ctcggcctcc tggtccgtgc ctgttccggc cgggtcctgc 107640ccggcgaggt ccagcagctg gtccaggagt ccgctgtcgc gcagccggtc cagggggacc 107700tgggcgaggg cggcgcgcac ggcccgctcc tcttcgccgg ctccgtccgt ggcgggggca 107760ccgggggtgc cggccgggag gagttgtccg aggatgtggg cggcgagtgc ggcgggcgtg 107820gggtggtcga acacgagggt cgcggacagg gtgagtccgc tgacggcgcc gagctggttg 107880cgcagctcga cggcgccgag ggagtcgaat ccgaggtcgc ggaaggcccg gtcggggccc 107940acctggtcgg ttccggtgag gccgaggacc tccgcggcgc gttcgcgcac cagcgtcagg 108000accgtctggg tacggcggct ctcgggcagc ctggccagcc ggtcgcgcag cgagggaccg 108060gagtccgcgg cctcgccgcc ggcggatgcg gccttcttga gggcggcgta cgcgggcatc 108120tcggccagca ggctgctggg ccggacggtg gtgaacgcgc ggacgaaccg gtcggggtcg 108180acgtcggcga cgacggcgac cgggtcgctg cccgtgacga cttgccgcag cgcgaggacc 108240gcgaggtccg ggtcgagcgg ccggatgccg gtgcggcggg cgagcgcggc ggcgcggacg 108300ccgtcggcca tgccctcccc gccccaggcg ccgtacgcca cggaggtggc gggcagcccc 108360tcggccctgc gctgttcggc gagggcgtcg aggaccgcgt tggcggcggc gtaggtgccc 108420tggcccgggt tgccgacggc ggcggacgcc gaggagaaga gggcgaacac ctccaggtcg 108480tgggcgcggg tcagttcgtc caggagcagc gcggaggcca ccttggcccg gaacaccgtg 108540gcgaaccgct cgggggtcag gccgtccacg acgccgtcgt ccagcactcc ggcggtgtgg 108600atgacgccgg tgagcggcgc gtcctcgggg atcgccgcga ggacggcggc cagcgccttc 108660cggtcggcgg tgtcgcaggc ggtgatgacg ggttcggcgc cgagcgcgcg caggtcggcg 108720gcgagttcgc cggcgccggg cgcgtcgggt ccgctgcggc tgaggaggac caggcggcgg 108780gctccggcac cggccaggcg gcgggcggta cgggcaccga gggcgccggt gccgccggtg 108840atcaggacgg ttccggtggg cttccagtcg cggggcgtcc cgggccggac ggcggcgagg 108900cggcggccga agaccgcacc gggccggacg gcgagctggt cctcgccctc ggggtcggcg 108960aggacggcgg cgagccgttc ggcggtccgc gcgtcgacag cgagcggcag atcgacggtg 109020ccgccccagc gctgcgggtg ctccagcgcg gcgacccggc cgagtccgtg gacggcggcc 109080tggagcggtg cggcgacggt ctcggcgggc gagacggcgg cggcgccacg ggtgaggcac 109140cacagcgggg cgtcggtgcg ggcgtcgccg agcgcctgga ccagggtcgc ggtcagggcc 109200gcgccggccg ggacgccgcc ggggagggcg ccggtcccgt caccgggttc cccggcgacg 109260gcgagcaggg agaccacacc ggtgaacggc ccggtggcgt cgagccgttc gcggatcgcg 109320gcggcgagtc cggcgcggtc cttgccggag acctccagga cctgggcgcc cgcgccgagc 109380gcggccagga cggcggaggt ccacggatgg tcggcgtggt cggcgggcac gacggcgagc 109440caggtgccgg cgggggtgcc ggcggtggcc cggttcaggg gccgccaggc gatgcgctgg 109500cgccagccgt ccacggtggt ctggtcgccg tgcttgcggc gccagtcccg cagggccggc 109560aggacgcgca gcaacgcgtc gccgtcgacg ccgagttccc cggcgaggga gtcgaactgc 109620tcgtcctgga ccgccgccca gaaggcggcg tccccggcgt cggccgtggt gccggtggcg 109680gcggcgggtg cctcgtcggg ccagaaccgc agtcggcgga acgggtaggt gggcaggtcg 109740acgcggcggg cgccggtgcc gtcgagcagt gccgcccagt cgagtccctg accggccgcg 109800tgcagccggg ccacggcggt gatcagggcc tgctcctcgt cgcgtccggt gcgcagggcc 109860
gggacggccg tgggggccgg gcggttctcg tcggcgaggg tgtccagggt ctcctgggcg 109920aggccgcaca ggattccgtc ggggccgagt tcgaggtagc gggtggcgcc gcgtcccgcg 109980agggtgcgca caccgtcgcc gaagcggacc gcctcgcgga cgtgccggac ccagtacccg 110040gggtcgcaca cctgcgcgtc ggtggccgcg tccccggtga ggttggagac caggggcagc 110100gtcggcgggg cgtacgacac actccgggtg acccgttcga agtcctgaag catggcgtcc 110160atgtgcagtg agtgaaacgc atggctcacc cgcaaacgcc tagtcttccg gccctgttcg 110220gcgagggcct cggcgagcgc ggtgaccgcc ttctcgtcgc ccgagaccac cacggactcc 110280gggccgttga cggcggcgag gcagatttgg tcggtcaggt acggggcgat ctcgtcctcg 110340gtggcgcgca cggcgagcat ggcgccgccc gccgggagtg cctccatgag gcgggcgcgg 110400gcggagacga cgcggcaggc gtccggcagg gagaggaccc cggccacgtg ggcggccgcg 110460agctcgccga ccgagtgccc ggcgagcagg tcgggccgta cgccccagga ctcgaccagc 110520cggtacaggg cgacgccgag ggcgaacagg gcgggctggg tccagccggt ccggtccagg 110580agcgccgcct cgggggtgcc ctcgtcggcg aacatcacgt cgcgcagcgg gcgttcgagt 110640tcctggtcga agtgggcgag gacctcgtcg agggcggtgg cgaagaccgg gaagcgggcg 110700tagagggcgc ggcccgcccc ggggcgctgg gagccctgcc cggggaagag gaacgcggtc 110760cggccgccgg gggccgcgcg gccgcgcagt acggcggggt gggcgcggtc ctcggcgagg 110820gcggtgagcg cgtcgaggag gtcgtcgcgg ccggccacgg cgaggacggc ccggtgctcg 110880aaggcggagc gggtggtgcc cagggagagc gccaggtcgg cggtggccgg cggctgcgcc 110940gcaccggcgg tgagccggtc gcggagccgg gcggcctgct cggtcaggga gtccgcggtg 111000gccgccgaca gcggcagggg cagcagccgt gcggcgtcgg tggcgtgcgt ggcgggctcc 111060gtgtcccgct ccggggcggc cgggccggtg ggggcctgtt ccaggatgac gtgcgcgttg 111120gtgccgctgg cgccgaagga ggagacagcg gcgcggcgcg ggcgtccggt ctcgggccag 111180tcggccgcct cggtcagcag gtggaccgcg tccgggtccc aggcgacgtg gcgcgagggc 111240ttctcggcgt gcagggtgcg cggcagcgtg ccgtgccgca tggcgaggac catcttgatg 111300atgccggcga cgccggaggc ggcctggctg tgaccgatgt tggacttgac cgagccgagc 111360agcagcggct gcccctcggg ccggtcctgc ccgtacgtcg ccaggagggc ctgggcctcg 111420atggggtcac cgagggtggt cccggtcccg tgtgcctcca cggcgtcgac ctcgcgggcg 111480gtgagtccgg cggcggccag cgccttgcgg atgacgcgct gctgggaggg gccgttgggg 111540gcggtgagac cgttggaggc gccgtcctgg ttgatggcgg agccgcgcag gaccgcgagg 111600acctcgtggc cgcgccggcg ggcctccgag aggcgctcga cgacgagcat gcccacgccc 111660tcggaccagg ccgtgccgtc ggcggcgtcg gagaagggct tgcagcggcc gtccggggag 111720agggcgccca tctcgccgaa ctcgacgaag ccgaccgggg tggacatcac ggtgacaccg 111780ccggccaggg cgagcgagca ttcgccggag cggatcgcct gcgcggcgag gtgcagggcg 111840gtcagggacg acgagcaggc cgtgtcgacg ctgacggagg ggccctccag gccgagggtg 111900taggagagcc gtccggagag gaggctcgcc gactgggcgg tctcgacgtg cccggactgc 111960ccgatggcgg gacggtagtc gccgctgccg ccgccgacga agacaccggt gtcgccgccg 112020cgcagcacgg cggggtcgat cccggcccgc tccagcgcct cccaggcggc ctggaggacg 112080atccgctgct gcgggtcgat gaccagggcc tcgcgcgggg agatcccgaa gaaggcgggg 112140tcgaagtcgg cgacgtcgta gaggaatccg ccctcgcggg tgacgctgcg gccccggccg 112200tcctcgtcgc cgtggagcag cttctccagg tcccagccac ggtcggtggg gaagccgccg 112260atggcgtcgg taccggcccc gaccagttcc cacaggtcct cgggcgagcg gacgtcgccg 112320gggtagcggc agctcatgcc gacgatgacg acggggtcgt ccgcgtcggc ggcggcgcgt 112380gccacggggg cgccggtgcc gggcccggtg tcctcgccga ggagttcggt gaggaggtgg 112440
gcggcgagcg cggcgggggt cgggtagtcg aagaccaggg tcgcgggcag ggtgagcccg 112500gtggccgtgg tgaggcggtt gcgcaggtcg accgcggtga gcgagtcgaa gccgaggtcg 112560ttgaaggcgt tctcggccgg gacggcggcg gcgccggtgt ggcccagcac cgaggcgatc 112620tcggtgagca cggtgtcgag gagggtgcgc ggacgctcgg aggggtcgga ctgccgcagc 112680cgctcgcgca gcgcggaggc ggtaccggcg tccgcgccgt cgccaccggt ggtgctcagg 112740gcctcgcggg cctcgggcac gccggtgagg agcagaccgg cgcgggtgcg ggtgaacgcc 112800ggggcgaacc ggtcccaggc cacgtccgcg acggtcaccg aggcgggagc ggccgggttg 112860gcggcggcct cgccgatcgc gggggcgagg gcgtcgagcg cgcgggccgg ctccatggcg 112920ggcaggccgt tggcgcgcag gtgggcggcg agaccgtccg gcccggcgcc ctgccaggcg 112980ccccaggaga ccgagacggc gttggtgccg tgggcgcggt gtcgctggac gagggcgtcc 113040aggcagactc cggccgcggc gcccgcgccc tggccgccga cgccccagac ggcggagatg 113100gagccgaaga cgacgaaggc gtccaggtca cggtcgccga cggcggtggt cagcgcctcg 113160taggcggcgc gcgccccggt gacaccgggc gtctcctcgc cggtgtcccc ggtgtggagg 113220acggcggtca gcggccggtc gtcggcctgc gccgccagcg cctcggtgag cggggcggcg 113280tcggccgggt cggggtggcg gacgacggtg agggtggcgc cgagcgcctc cacctcggca 113340cgcagggcgg tggtggcggc gtcctcgggg ccggccgggc cggcgagcag caccccggtg 113400gcgccgtgcg cggcgagcca gcgggcgacg tgcccgccga agccctccgc ccggcccgtg 113460accaggacgg tcccggcggg gtgccaggtg atgtccggag cgggagcggc ggcccgggac 113520agccggcggc cgaaggcgcc ggaggcgcgc agcgccacct ggtcctcggc cgggccgtcg 113580gtgcgggcga cggtcaggac ggcgcgcaga cggtcgacgg tgcgggcgtc ggcgacgtgg 113640tcgacgtcga ccagtccgcc ccaccgttcg ggctcgtcca gggcgagtac ccggcccagg 113700ccccacaggg cggcgcggtc cgggtcggcc gccgggtcgg agcggccgac ggagacggcg 113760tcgcgggtga cgcaccacag cggggcgtcg atcccggcgg ccgccagcag gcggagcagg 113820gtgtcggggc gggcggtgcc gtccgtcgcg gtgccggcga gcagcgagag gacaccggcg 113880tacgcggtgc cgtcggcggc cccgtcgagc agggcggcgg tgccgtcctc gtcggcgggg 113940acggcgaggt ggacggtgtg ctcgccgaag gtggcggcca gggcctgggt ccacgcgtcg 114000tcgtgcccgg ccagggtcag gaccagccag ggtcggcccg ttccgtcgtc ggcggtgttc 114060ccgaggggga cgggctgcca ggtgacgcgg tagcgccagc cgtcgagcag ggtgcgctcg 114120gacaggccac ggcgccaggt ggagagggcg ggcagcacgt tcgacaggac cgcgtcgtcc 114180aggtcgaggg tggcggagag cgactccagg tcctcgcgct ccacggcggc ccagaacgcg 114240gcgtccgccg ggtcctgcgc ggtggccgtg tgcggcccgg tggccggctc gggccagtac 114300agctcgcgct ggaaggcgta ggtcggcagg tcggtgcggc gggcgccggt gccggtgaac 114360caggcggtcc agtcgacggc gacaccggcg gtgtggagcc gggccagggc ggtgagcagc 114420gagtgttcct cgggtcggtc cttgcgcagc gccgggacgc tcacgccgcc gcttccgtcg 114480gcggccgtgt cgaggacccg ggcggccatg ccggtgagga ccccgtcggg gccgatctcc 114540aggtaggcgt cggctcccgc ctcggcgagg gcgcggacgc cgtcggcgaa gcggacggtc 114600tcgcggacgt gccgcaccca gtactcgggg tcggtgagct gaccgggctg ggcgagggcg 114660ccggtgacgt tggagaccac cgggacgcgc ggctcggcgt actccaggct ctccgcgacc 114720cggcggaact cggcgagcat cggttccatc agcgccgagt ggaaggcgtg actgaccggc 114780agaggctgcg tcttgtgccc ctcccccgcg aactcggccg ccagggccag gacttcctcc 114840tgctcaccgg cgatgacgac ggagtcggga ccgttcacgg cggcgaggga caggccctcg 114900gtcagccggg gcacgacctc ggcctcggtc gcccgcacgg ccaccatcgc cccgcccgtg 114960gggagttcct gcatcaggcg ggcgcgggcg gcgaccagcg cgcaggcgtc gtcgagcgag 115020aagactcccg ccacgtgcgc cgccgcgatc tcgccgaccg aatgacccgc cacgaactcc 115080
ggcgcgacac cccacgactc caccagccgg aacaacgcca cctccaccgc gaacagcgcc 115140ggctgcgtgt acccggtcgc ctccagcagc gccgccgcct cggaaccctc ctcagcgaac 115200agcacctccc gcagcgaacg ctccagcccc gtgtccagac gggccaccac cgagtccagc 115260gcctccgcga acaccgggaa acgctcgtac aactcccggc ccatccccgc acgctgcgaa 115320ccctggcccg agaagagcag ggcgaggcgg cgttcccagg cgcggtcgcg gacggtctcc 115380aggggttcgc ccccggcggt gaccaggtgg acggcgcggt gggcgaaggc ccctcggccg 115440gtggccaggc tgtaggcggt gtcgagcgcg gggacctcgg gccgggcctg tgtgaaggcg 115500gtcagccgcg cggtctgggc cgccagggcg tcctccgagc gggccgagac cagccagggc 115560acgacggacg gctcggtgac cggttccggg gtctcggcgg tggccggttc gggctgctcc 115620aggatgacgt gcgcgttggt gccgctcacg ccgaaggcgg agacgcccgc ccggcgcgca 115680cggcccgtct cgggccaggc gcggtgctcg cggagcagtt ccacggtgcc gtcggaccag 115740tcgacgtgcg aggacggcgc gtcgacgtgc agggtgcgcg gcagttcgcc gtggcccagg 115800gcgaggacgg tcttgatgac accggcggcg cccgcggcgg cctgggtgtg gcccaggttg 115860gacttgacgg tgccgagcag cagcggctgc tccgggtcgc ggccctcgcc gtaggcggcg 115920atcagggcgt gggcctcgat cgggtcgccc agggtggtgc cggtgccgtg cgcctcgacg 115980gcgtcgacct ccgccggggt gaggtgcgcg tcggccagcg cctggcggat gacgcgctgc 116040tgggcgcggc cgttgggcgc ggtgaggccg ttggaggcgc cgtcctggtt gacggcggag 116100ccgcgcagca cggcgaggac cgggtggccg tgacgacggg cgtcggagag ccgctccagg 116160acgaggacgc cggcgccctc gccccaggcg gtgccgtccg cggcgtcggc gaacgccttg 116220cagcggccgt cgccggccag gccgccctgc gcggtgaact ccacgaaggc gtccgggctc 116280gccatcaccg agacgccgcc ggcgagggcc agggtgcact cgccggagcg cagcgccccg 116340gcggccatgt gcagggccac cagcgccgag gagcaggcgg tgtcgacggt gaccgccggg 116400ccctccaggc ccagggtgta ggacaggcgg ccggacatca cgctggcggt ggtgccggtc 116460gcggcgtggc cgcgcacgtc ggtggtgccg cgccgcagca cggtggcgta gtcctggccg 116520ttggtgccga cgaagacgcc ggtggcgctg gagcgcaggc ccgtcgggtc gatgccggcc 116580cgctccaggg cctcccagga ggtctccagc agcaggcgct gctgcgggtc catcgcaagg 116640gcctcgcgcg gggagatgcc gaagaacgag gcgtcgaaga gtccggcgcc ctggaggaag 116700ccgccctcca gggtggcgga gccgccacgg gccagcgagt ccaggtccca gccgcggtcg 116760gccgggaagc cggagacggc gtcgcggccc tcgccgagga gctgccagag ctcctcgggc 116820gactcgacac cgccggggaa gcggcagccc atgccgacca cgacgaccgg gtcggtctcc 116880gtggcgcggg gagcctcgcc ggccgcgttc ccgcccgccg ccggctcggg caggtcgccc 116940aggatctcgg tcaggaggtg agcggccagg tcgcgggcgg tcgggtggtc gtagacgagg 117000gtggcgggca gccgcaggcc ggtggtggcg cccagggtgt tgcgcagttc gacggtggtg 117060agggagtcga agccgaggtc ggtgaaggcc tggtcggcct cgacggcgtc cgggtcgggg 117120tgaccgagga cggcggcgac ctgggcgcgc aggaagtcca gcacgtaccg ctcgcgggcg 117180gggccggtca gggcggcgac ctggcggcgc agcgccggtt cggtgtcgcc gtgggcggtg 117240gccgcgccga cggtggcgcg tacctcgggc aggtcgccga cgagggcgcg gtgcggggcg 117300aagacggtgg cgtagcgctg ccagtcgatg tcggcgacga ggagcgcggt gtcgcggtgg 117360ccgacggcgg cgtcgagggc ggcgagggcg tcctcggggc tcatcggggt gaagccgcca 117420cggcggaccc ggtcctcgac gccggtgccc tcgccggcca tgccggtgcc ggaccagggg 117480ccccaggcca ccgaggtggc aggcaggccc tgttcgcgcc ggtgttcggc gagggcgtcg 117540aggtaggcgt tggcggcggc gtagttggcc tggccggcgg cgccgagggt accggcggtc 117600gaggagaaca ggacgaagtc ggtgaggccg aggtcgcggg tcagctcgtg caggtgggcg 117660gcggagcggg ccttggcgtc gaggacggcg gcgaaccgct ccggggtgag ggtgtccagg 117720
aggccgtcgt cgaggacgcc cgccgcgtgc accacggtgg tgagggggcg gtcggcgggc 117780acctcggcca ggagcgcggc gagcgcctcc cggtcggcgg tgtcgcaggc ggcgagggtg 117840acctcggcgc ccagttcccg cagctcggcg gcgagttcgg cggcgccggg ggcgtccgcg 117900ccgcgccggc tggtcagcag caggtgcggc gtgccgcgcc cggccaggag ccgggcgacg 117960tgggcgccga gggcgccggt accgccggtg atcaggacgg tgccctcggg ggcgaacgga 118020cgcgccggtt cctggggcgc cgggtggtgg gcgatccgcc gcgcgtaggt ggcggaggcg 118080cggacggcga gctggttctc cgtgccgggc acggccagga ggtgcgccag ccggcgggcg 118140acgcgggtgt cgaactgctc gggcaggtcg accagggcga ggcggcgtcc ggggttctcc 118200agggcggcga cccggccgag cccccacaca cccgcctgcc cgggacgggc cagccggtcg 118260gcgcgtccgg tgctcacggc ctgccgggtg acgatccaca ggggcgcggt gatcccggcg 118320tcgtccaggg cgttcagggc ggcggccgtc ggcgccacgg cgcccgtccc tgccacgtcg 118380tcggcggtgg cggcggtgtc gagggccggc agggcgagga cgccggtgaa gccgtcctcg 118440gggcgcggca gccggcgcag ggtgtcggcg agcgcggcac ggtcgggggc cgcgaggtcg 118500acggtgacct cggcggtgtc caggccgagg gtgtccagga gggcggcggt ctcgggcgcg 118560gccgtaccgg tgggacgcag gaccagccag gtgccctcgg gccgtccggt gggggcgccg 118620tcgagcggct tccacacgat ccggtggcgg cggccgtcgg cggcggcctg tgcggtgcgg 118680cggcgccgcc acgaggacag ggccggcacc agggcggaca gcgtgtcgtc gtccaggccg 118740aggtcggcgc cgagcgtggg caggtcggcc cgctcgacgg ccgaccagaa cgcggcgtcg 118800gcgccggtgt cggcggggcc cgcggccgtg gccggggtga aggtggtgtc gggccagtag 118860cggctgcgct ggaaggcgta ggtgggcagg tcgacgcggc gggcgccggt gccctcaaag 118920gcgccggtcc agtcgacggc ggtgccgtgg acgtgcagcc gggcgagggc ggcggtgagg 118980aacggcacct cgtcgcggtc gcggcgcagc gcggggacga cggtgacggg ccgggcgccg 119040gtggcgtcgg ccgggtcggc gtcgaggacg tcgcgggcgg cggcggagag ggtgccgtcg 119100ggaccgagtt ccaggcagga ggtgacgccc tggcgggcga gggtgcgcac ggcgtcggcg 119160aaccggacgg tggcacggac ctggccgacc cagtactcgg gcgaacgcag ttcctcgggg 119220gtggcggggg cgccggtgac ggtggagacc accggcacga gcggcgtctc gtaggtgagg 119280ccgcgggcga cgcgggcgaa gtcgtcgagc atggcgtcca tgcgcggcga gtggaaggcg 119340tggctgacct tcagcgcgcg ggtgcggcgg ccgtcctcgg cgaggcgggc ggcgacggcg 119400gcgaccggct cctcgtcacc ggcgatcacc acggcgcgcg ggccgttgac cgcggcgagg 119460gagatccggt cctcgcctcc ctccaggagg ggcagtacct cttcctcgct cgcctcgacg 119520gcgaccatgg cgccgcccgc cggaagggcg gccatgaggc ggccacgggc ggcgacgagg 119580gcgcaggcgt cgtccaggga gaagaccccc gccacgtggg cggcggcgat ctcaccgacg 119640gagtggccgg cgacctggtc ggggcgtacc ccccaggact cgacgagccg gtacagcgcc 119700acttcgaggg cgaacagggc gggttgcgtg tacgccgtgt cgtccaggag cgcggcctcg 119760gcggtgccct cctcggcgaa gagcacctcg cgcagcgggc ggtccagttc ctggtcgaag 119820cgggtgagga ccgcgtccag ggcttcgccg aagaccgggt gacgctcgta caggtcccgt 119880cccatggccg ggcgctggct gccctggccg gtgaagagga aggcgagccc tcctcgtgcg 119940ggggcgccgg tgagcagcga ggcgtcgggg gcgccctcgc gcagcgcggt gagcgcccgc 120000agcaggccgt cccggtcgcc ggtgaggacg gcggcgcggt gctccaggac gccacgggtg 120060gtggccagcg agtgggccag gtccaccagc ggtacgtccg gacggccggc gaggtgtccg 120120gcgagccgct cggcctgctc gcgcagggcg gccggggtcc gggcggagag cacgacgggg 120180acgcccgggg gcagggtggc cgggtcgccg gcgggggccg gggacgcgga gggctcggtc 120240gcgggctcct cgggcgcctg ctcgacgatg gtgtggacgt tggtgccgga gaggccgaag 120300gaggagacgg cggcccggtg cgggcgtccg gtctcgggcc acggcacggt ctcggtggcc 120360
aggtcgatgg cgccggagcc ccagtcgacg tggctggagg gggtgtcgac gtgcagggtg 120420cgcgggacgg tgccgtgccg catcgccatg accatcttca tgacaccggc gatgccggag 120480gccatctggg tgtggccgat gttggacttc accgagccga gcagcagcgg ccggtccccg 120540ggccggtcct ggccgtacgt cgccagcagg gcctgggcct cgatggggtc gccgagggcg 120600gtgccggtgc cgtggccctc gaccacgtcc acctcgcccg gggtgagccg ggcgttggcg 120660agggcctggc gtatgacgcg cgcctgggag gggccgttgg gggcggtgag gccgttggag 120720gcgccgtcct ggttgacggc ggagccccgg atgacggcga ggacggggtg gccgttgcgg 120780cgcgcgtcgg acagccgctc gacgaggacc aggccgacgc cctcggccag gctcatgccg 120840tcggcgccct cggcgtacgc cttgcagcgg ccgtcggggg ccatcgcgcg ctggcggctg 120900aagccggtga acgcctgcgg ggtggccatg acgctgacgc cgcccgccag ggcgagggtg 120960gactcgccgt tgcgcagcga ctggcaggcc aggtggagcg cgaccaggga ggaggagcag 121020gcggtgtcca gggtgacggc cgggccctcc aggccgaagg tgtaggcgag ccggccggac 121080atcacgctgg agatggtgcc ggtgatcagg tggtcctcgg cgccctcgga gccggcgccg 121140cccgcggtgt agtcctggta gctggcgccg atgaaggtgc cggtcatgct tccgcgcagg 121200gtcgcggggt cgatgcccgc gcgctcgatc gcctcccagc cggtctccag cagcaggcgc 121260tgctgggggt ccatcgccag tgcctcacgc ggggagatcc cgaagaagcc ggcgtcgaag 121320tcggcgacgt cgtggaggaa tccgccctgc gtggagtagg tggtgccggg ccggtcgggg 121380tccgggtcgt agagggcgtc ggcgtcccag ccccggtcgg cggggaagcc ggtgatggcg 121440tcggcgccgg ccgcgatcag ctcccacagg gcctcgggcg aggtgacgcc gcccgggtag 121500cggcagctca tcgcgacgac ggcgatcggc tcgtcgtcgg tggcggccgt ggtgcggacc 121560gggccgtcgg ggccggcggc gggaccgccg gccaggcccc gcaggtggcc cacgagggtg 121620agcgggtccg ggtagtcgaa cacgagggtg ctcggcaggg tgaggccggt gacggaagcc 121680agccggttgc gcaggtcgac cgcggtgagc gagtcgaacc cgatgtcacg gaaggcgcga 121740cgctccgaca gcacctccgg ggaggcgtgg ccgaggacgg tggccgcctc cgagcggacg 121800agttccagca ggaggcggtc ctgctcgccg gcgggcaggg agcgcaggcg gtggatgaac 121860tctccggcct cgccggcgcg ggcctcctgc tgctcggtga ggcggcggac ctcgggcacg 121920ttctcgaaga gttcggtggg ccgcgaggag gtgaagacgg ggtggtagcg gtcccagtcg 121980acgtcggcga cggcgacggc gccctggtcc ccgtcgagga cctgccggag cccggcgagg 122040gcgaggcgcg ggtccatgaa ctccaggccg ctgcggcgga tctggccggg gtcgacccgg 122100ccgagcttca tgtcgtcggc ccagatgccc caggagacgg cggtgccgtg ggcgcctcgg 122160gcgcaccggt gctcggcgag cgcgttgacg tgggcgttgg cggcgacgta cgcggcgtgc 122220cggccactcc cccacatacc ggcggtggac gagtagagga cgaaggcgtc gagctggtcg 122280tcgtcgagga gttcgtcgag gtgggcggcg cccgcggtct tggcgtggac gaccttggcg 122340aacgcgtcga ggctggtctc ctcgatggac tggagctcga tgacggcggc ggtgtggaag 122400acgctgcgga cggtgcggcc ctcggcggtg aggccgtcga ggagcgcggc gaccgcctcg 122460cggtcggtga cgtcgcaggc ggcgacggtc acccggcagc cgcgttcgcc gagttcggcg 122520gcgagttcgg cggcgccggg ggcgtccgcg ccgcggcggc tggtgaggac caggtcgcgg 122580gcgccctggt cggcgagcca gcgggccagg tgcgggccga gcgttccggt gccgccggtg 122640accaggacgg tgccgcgcgg gctccagcgg ccggtgccgg tgggcgccgg ggccggggag 122700acgcggcggg ccaggacgcc ggaggcgcgg acggccagct ggtcctcgcc ggtggtgccg 122760gccaggacgg cggcgaggcg ttcgcccgcc cggcggtcca gggtctcggg caggtcgacg 122820gtgccgcccc accggtccgg gtgttccagg gccgcggtcc agccgaagcc ctggacgagg 122880gcctgggcgg gccgggtgag gcggtcggcg cgcccggtgg agacggcgcc gcgggtcagc 122940gcccacaggg gtgcctcgat cccggcgtcg ccgagggcct gcgtcagcgt gacgctgagg 123000
gcgagaccgg tggtgaggac cgggtgggcg gcgccggtgg actcgtcgtc ggccagcagg 123060gagaccaccg cggccggttc gggcaggtcg cgcagccgct ccgtgagagc cgcccggtcg 123120gcgtcggccc cggtcagcgc gaggggcacg gtccgcgcgc cgtgcgcggt cagctgctcc 123180gccacggcgg cggcgtactc ggggtcggtg ccctccgcgg tgacgagcag ccaggtgccg 123240tccagggtcg cggaggggag cgcgccgagc ggcttccagg tggcgcggta gcgccaggac 123300ccgacggtgg aacggtcgcg cctgccacgg cgccaggtgg agagggcggg cagcagcggg 123360gcgagcgcct cccggtcgag ttccaggcgg gaggcgaggg actccgcgtc ctcctgctcg 123420acctcggccc agaactcggc gtcggcgggg tcgccgtcgc cggagccctg cgggcgggcg 123480gagacggccc acaggtgctc acgctggaag gcgtaggtgg gcaggggtgt cctgcgggcg 123540ccggtgtccg cgtagaccgc gttccagtcg gcccggccgc cgcgtaccca ctgttcggcg 123600agggaggtga ggaagcgcgc ggtgccgccc tgttcgcggc gcagggtgcc gacggcggcg 123660gccgggcggc cggcctcctc ggcggtggcc tggacggcca tggtcagcac ggggtgcggg 123720ctgatctcga cgaagagcgt gtgttcggtg tcgagcaggg cgcgcacgcc gtcctcgaag 123780cggacggtct gccgcaggtt gcggtaccag taggcggcgt ccatgccggt ggtgtcgagc 123840gtggcgccgg tgaccgtgga gtggaacggg acccgggcgg cgcgcggtgc caccggggcg 123900aggaggtcga ggagttcggc ctcgatccgc tccacctgtg ccgtgtggga ggcgtagtcc 123960acggcgatcc ggcgggcacg caggtcgcgg gcggcgaact ccgcctggag ggcgtccagg 124020gcctcgggcg taccggcgag gacgacggag cgcgggccgt tgacggcggc gacggagacc 124080gctcccgcgt ggggggtcag gtactgctcg gcctcggcgg cggagacctg gacggacatc 124140atgccgccgg ccccggccag gacccggcgg atcgcctggg agcgcagggc gacgacgcgg 124200gcgccgtcgt cgagggtgag ggcgccggag acgacggcgg cggcgatctc gccctgggag 124260tggccgatca cggcgtcggg ggtgacgccg tgggcccgcc acagctccgc gagggagacc 124320atcacggccc aggtcgcggg ctgcacgacg tcgacccggt ccagggtcgg cgcgccgtcg 124380gcctggcgga cgacgtcgac gaggtcccag tcggtgtacg gggccagggc ggccgcgcag 124440gcgtggagcc gctcggcgaa caccggtgac gtgtcgagga gttcggcgcc catgccggcc 124500cactgggagc cctggccggg gaagacgaag acggtgcggc cgtcggtgtc ggcggtgccg 124560tggaccacct ggccggtgga ctcgccggcg ccgaacgcgg cgagcgcgtc ggtgagttcg 124620ccggcgtccg cgccgagggc gacggcgcgg tgctccagga cggagcgtgc ggtgatcagg 124680gtgtggccga cgtcggcggg gtgcggtgcc gcgccggagg tgacgtgggc ggcgaggttg 124740gcggcctgct cgcgcagggc ggagggggcg gcggccgaga ggatccacgg cagggtgccg 124800cccgtgggca gggcgggtcc ggcggcgggg gcgggctcct cggcgggggc cgcctgttcc 124860aggatggcgt gcgcgttggt gccgctgatg ccgaaggagg agacggcggc gcgcagcggg 124920cggtcgtccc gggcccagcc ggtgttctcg gtgacgaggc ggacggtgcc gccggaccag 124980tcgatgtggc tggagggctc cgtggcgtgc agggtgcgcg gcagggtgcc gtggcgcatc 125040gccatgacca tcttgatgat gcccgcgacg ccggcggccg actgggtgtg gccgatgttg 125100gacttcaccg agccgagcag cagcgggttc tcggggtcgc ggtccctgcc gtaggtggcg 125160tgcagggcct gggcctcgat ggggtcgccg agggcggtgc cggtgccgtg tgcctcgacc 125220gcgtcgatgt cgtcggtggt gaggccggag gcggccagcg cctggcggat gacgcggacc 125280tgggaggggc cgttgggggc ggtcaggccg ttggaggcgc cgtcctggtt gatggcggag 125340ccgcggacga cggcgaggac ctcgtggccg ttgcggcggg cgtcggagag ccgctccagg 125400acgaggatgc cgacgccctc ggcgagggtc atgccgtcgg cgctctcgga gaaggccttg 125460cagcggccgt cccgggcgag ggcccgttgc cggctgaagg cgatgaacgg catcgggttg 125520gtcatgacgg tggcgccgcc cgccagggcg agggtggtct cgccgttgcg cagcgactgg 125580caggccaggt ggagcgccac cagcgacgag gagcaggcgg tgtcgacggt gacggccggg 125640
ccctccaggc cgaaggagta ggcgaggcgg ccggagagca cgctggggct ggagcccgtg 125700acggcgtggc ccgcggaccc gtcgtccatg gagacgccgt agtcctggta cgtggagccg 125760atgaaggtgc cggtgggcgt ggagtgcacg gcggccgggt cgatgcccgc gtgctcgaac 125820gcctcccagg tggtctccag caggaggcgc tgctgcgggt ccatggagac ggcctcgcgc 125880ggcgagatgc cgaagaaccc ggcgtcgaac tcgcccgcgt cgtgcaggaa tccgccctgc 125940gtggagtagg tggtgccggg ccggtcgggg tccgggtcgt acagcgcgtc gccgtcccag 126000ccccggttga cggggaactc cgagatcgcg tcggtgttgt cggcgacgag ctgccagaag 126060gcgtcggcgg agcgggcgcc gccggggaag cggcagctca tgccgatgat ggcgatgggc 126120tcgtcggtga cggcggggcc ggtcgccacg ggggcggtct cgaccgtgcc gaggagttcg 126180gcacgcaggt ggcgcgcgag ggcgagcggg ttcgggtagt cgaagaccag ggtgctcggc 126240agcgcgagtc cggtgaggtg ggcgaggcgc ttgcgcagtt cgacggcggt cagcgagtcg 126300aacccggcgt cacggaacgc ctttttgacc ggcaccccct cggcggaggt gtggccgagc 126360accacggcgg cctcggcccg cacgaggtcg acgagcagcc gctcctgttc ctgctcggtg 126420aggccgccga ggcgctccgc cagcccggag acggtgccgg tgctcccggg agcagcgagg 126480gccgcgacgg tgtcgacggt gtcgaagagg cggctggacc gggcggcggt gaagaccggg 126540tggtactgct cccagtcgac gtcggcgacg gtcacggtgg tctcgccgcg tacgacggcc 126600cggcgcagct ccgtcatggc ggtggcgggg ttcagcagcc gcaggccggc gcgttccagg 126660ttctccgaga cggtccggtg ggcgctggcc atgccgacct cggcccaggg gccccaggcg 126720acggaggtgg cggcgaggcc gcgcgagcgg cggttctcgg cgagcgcgtc gaggtaggcg 126780ttggcggcgg cgtaggcgct ctggcccgcg ctcccccaga ccccggcgac ggaggagacc 126840aggacgagga agtcgaggtc gtggccgtcg aggagggcgt cgaggtgggc ggcgccgagg 126900gtcttggcgg ccatggcggc ggcgacctgg tcgagcgggg tgtcggcgag cggcgcggcc 126960tggccgacgc cggcggcgtg cacgacgccg gtgagggggg cgtcctcggg caccgcggcg 127020aggacggcgg cgagggcgtc gcggtcggcg ctgtcgcagg cggtgagggt gacgcgggcg 127080cccagttcct ccagttcggc gcggagttcg gcggcgccgg gggcgtcggg gccgcggcgg 127140ccggtgagga cgaggtgggc ggcgccggag cgggcgagcc agcgcgcggc ctcggcgccg 127200agaccgccgg tgccgccggt gaccaggacg gtgccggagg cggtgaactc gtcctcgcgg 127260ggcagggcgt cgccggggtg ccggacgacg cggcgggcca gggtgccgcc cgcgcggacg 127320gcgagctggt cctcgccgtc gcgggtggcg aggaggctga ccagccgctg ggtggccggg 127380gcgtcgagga cctccggcag gtcgaccagt ccgctccact gctcggggcg ttccagggcg 127440gcgacccggc ccaggcccca ggcggcggca cgggcggggt gggtgagcgg gtcgccgggg 127500ccggtggaga cggcaccgcg ggtgaccgtc cacagcgggg cggtaacggc ggcgtcgccg 127560agggcctggg cgaggaccac gccgagggcg aagccggccg ggagtccgcc ggccggtgcg 127620tcggtgccgg tggcctcggg gcgggcggcg tcggcgagcg gcagcagcga caggatgccg 127680cggacaccgg cggcgccttc ggccgtggcg ttctcgacgg cgtcgcgcag ggtggtggtg 127740aggaggtcgc ggtcgaggtg ggaggcgtcg agcaccaggc gctcgacgac ggcgccgtgg 127800ccgcgcaggg cggcgaggac ggtgtcggcc gcgccgtcgt cctcggtggt gacggccagc 127860caggtcccgt cgagcaccgg ggcgctctca gtaccggcgg gctgccaggc gatccggtag 127920cgggccgcgt cgaggacggc gcgttcctga cgggcgcggc gccaggagct cagcgacggc 127980agcagcgtgt agagggaggc gtgttcctcg tcgcgcagac cgaggagccc ggcgagcccg 128040gtggcgtcgc cgcgttcgac ggcggtccac agctcggcgt cggccgggtc gccggagccg 128100tcaccggtgc ggcgcacggt cggcgcggtg gtgtccggcc agtaggtctc gtgctggaag 128160gcgtacgtag gcagctcgac ggtgccggcg cctgccgggt ggaccgcgcc ccagtcgacg 128220cggacgccgc ggacgtggag ccgggcgagg gcggtgagga cggactcgtc ctccggtcgg 128280
ccggtgcgca gcgtcgggac ggcgtcggcc tcgctgtcgt caccgagggt gtcctgggcg 128340gcggcgcaca gggagccgcc ggggcccagt tcgaggtagg tggtggcgcc ggcctcgtgg 128400gcggcgcgca cggcgtcggc gaagcggacg gtggagcgca cgtggcggac ccagtagtcg 128460gcggtccgca ggtggtcgcc ctcggcgagg cggcccgtga ggtcgcaggc cacggggacg 128520gtgggctcgt ggtacgtcag gccctcggcg acctcgcgga aggcgtccag cacggcgtcc 128580atgcggggcg agtggaaggc gtggctgacc cgcagccggc gggtggcgcg gccgaggccg 128640gcgaagtgcg cggcgatggc cgcggtggcc tcctcctcgc cggagacgac gacggccgac 128700ggcccgttga cggcggcgag ggcggccacg tcctcctgcc cctggagcag gggcaggacc 128760tcgtcctcgg acgcctggag cgaccacatg gcgccgccct cgggcagggc ctgcatcagg 128820cgggcgcggg cggcgaccag cgcgcaggcg tcgtccaggg agaggacgcc tgcggcgtgg 128880gcggcgccga tgccgccgac ggagtggccg aggagcacgc cggggcgcac gccccaggac 128940cgcaccagcc ggtacagcgc ggtctccagg gcgaacaggg cgggctgtgt ccagccggtc 129000tcgtacaggg cggcggcgcc cggggtgccc tcctcggcga acatgacgtc cttgagggag 129060ccgtgcgggg tgccggcggg ggcgtcgacg aggagcgggt ccaggcgcgc gacgacctcg 129120tccagagcct cggcgaagac cgggtggcgc tcgtacagtt cgcggccggt gccggggcgc 129180tggctgccct ggccggtgaa gagcagggcg gtgcggccgg tgccaccggt ctcgccgagg 129240acgagcgccg ggtcggggcg gtcggcggcg agggcggcca gggcacggcg ggcgtcgtcg 129300gcggtgccga cgaccacggc gcgctgctcg aaggccgggc gggtggtggc cagcgagtgg 129360gtgaggtcgg cgggggcctc gtcggggcgc gcgtcgaggt gggcgagcag ccgggcggcc 129420tgggcgcgca gcgcgggccg ggtgcggccg gagagggtcc agggcaggac ggtggcttcg 129480gcgggcgggg tgccggtgga cccggcgggg gcggactcgc cctgttccag gacgacgtgg 129540gcgttggtgc cgctgatgcc gaaggaggag acaccggcgc gccgggggcg gccggtctcg 129600ggccacgcct ccggctcctg gaggatgcgg acgtcgccgg tctcccagtc gacgtggcgg 129660gaggggctgc cggcgtgcag ggtgcggggc agttcgccgt ggcgcatcgc catgaccatc 129720ttgatgatgc ccgccacacc ggcggcggcc tgggtgtggc cgatgttgga cttcaccgag 129780ccgagcagca acggccggtc cccgggccgg tcctggccgt acgtggcgag gagggcctgg 129840gcctcgatgg ggtcgccgag ggcggtgccg gtgccgtgtg cctcgaccgc gtcgacgtcg 129900gcggtggtga ggccggccgt gtccagggcc tggcggatga cgcgctgctg ggaggggccg 129960ttgggggcgg tcaggccgtt ggaggcaccg tcctggttga cggcggaccc ccggaccacc 130020gcgaggacgc ggtggccgtt gcggcgcgcg tcggagagcc gctccaggac gagcatgccg 130080acgccctcgg accagccgac cccgtcggcc tcgtcggaga acgccttgca gcggccgtcg 130140ggcgacaggc cgcgctggcg ggagaactcg atgagggagg tcggcgtgga catcaccgtg 130200acgccgccgg ccagggcgag cgagcactcg cccgagcgca gggcctgcat cgcccagtgc 130260atggcgacca gggaggagga gcaggcggtg tcgacggtga ccgccgggcc ctccaggccc 130320agggcgtacg agacacggcc ggaggcgatg ctgggggcgg tcccgctgcc ctggtggccc 130380tcgaaacgct cgtcggcgag ggtcgccgag tagtcgctgt acatgacgcc ggcgaagaca 130440ccggtacggc tgccgcgcag gcccaccggg tcgataccgg cgtgctcgac ggctgtccag 130500gacgcctcca gcagcagtcg ctgctgggag tcggtggcga gcgcctcacg ggggctcatg 130560ccgaagaagc ccgcgtcgaa ctcgcccgcg tcgtgcagga atccgccgta gcgggtgtac 130620gaggtgccgg ggtggtccgg gtcggggtgg tagagggagt cgaggtccca gcctcggttg 130680acgggcaggc cgccgatcgc gtcggtgccc tcggtgacca ggcgccacag gtcgtcgggc 130740gagccgacgc cgccggggta gcggcagctc atgccgacga tgacgatcgg gtcgtcggcg 130800gtggcggacg tccgcggggc gggggcgggg gcctcggcct cgggggcgtc ggtcagttcg 130860gccaggaggt ggccggccag ggccgcgacg gtcgggtagt cgaagatgac ggtggcggag 130920
gtgcgcaggc cggtggcggc ggccagcccg ttccgcagct cgacggcggt cagggagtcg 130980aacccgagct cctggaaggg gcgttcgggg tcgatgtcgg cggcggaggc gtggccgagg 131040acggcggccg tccggtcgcg caccaggtcg acgacggcct ccgtgcgggc ctgttcgtcg 131100agccgggcga gccgctgggc gaggccgagg gcggcctcgg aaccggtgcg caccgtgcgg 131160cgggcgcggg tgcggaccag gccgcgcagg agcgggggca cctcgccccg gccacgcagg 131220gcggccaggt cgaggcggac gggcaggacg gcggcgcggg gcagggcgag ggtggcgtcg 131280aagagggcca ggccctgttc gacggtgagc ggcggcgtgc cggagcggga catgcgctcc 131340aggtcccgct cggagagttc gccggtcatg ccgccgtcga cgggcaccca ggggccccag 131400ccgagggaga ggccgggcag cccggcgtcg cgacgcaggc gggcgacggc gtccaggaag 131460gcgttgccgg ccgcgtagtt ggcctggccc ggggcgccgg tggtaccggc gagggaggag 131520aacaccacga aggcggcgag gtcgaggtcg cgggtggccc ggtgcagatg ccagacggcg 131580tcggccttgg gccgcaggac ggcggcgagc cgctcctcgg tgagggattc gaccagtccg 131640tcgtcgaggg tgccggcggt gtgcacgacg gcggtgacgg ggtgggcggc cggtacggcg 131700gcgaacagcc ggtccacggc ttccgggtcg gcgacgtcgc aggcggcgag agtgacctcg 131760gcgcccagcg cgccgaggtc ggcggcgagt ccggcggcgc cgggggcgtc ggggccgcgc 131820cggccggcga gcaccaggtg gcgtacgccg cgctcggtga cgaggtggcg ggcgagggcg 131880gcgccgaggc cgccggtgcc gccggtgacg acaacggtcc cctcggggtt ccaggcggcg 131940gcggtggtct cgccgtcggg gcggacgcgg gcgagccggg cggcgaacac ggtgtcgccc 132000cgcagcgcgg tctgcggttc ctcggcggcg agggccgcga cgagcgcggc ggggtcggtc 132060ccggcgacgg ggtcgaggtc gagcaggccg aagcggtggg ggtgttcggc ctgggcggag 132120cggaccaggc cccagacggc ggcggcggcc gggtcggtga gcggcccttc gccgacggcg 132180acggcgcccc gggtgacgaa caccaggcgc gccccggccc gggtgtcctc ggcgagccaa 132240cgctggatgt gcccgaggac gcgggcggtg ctcgcgtgga cctcggcggg tacggcctcg 132300gggtcgccgc cgccgacggg gacgagcacc agggccggcg tgtcgggtcc ggcggcctcg 132360gtgagcgggc cgacggtgac cccggcggcg gtgagggcgg cgccgagccg gagggggtcg 132420gggccgagca gaccgacggt gccgggggtt tcgggggcgt cggcgccgtg gaggcgggtc 132480cagtcgaggt ggaagagggc gtcgcgctcc agcacgccgg cggcggcccg gcgcgcgtcg 132540ggggcggagc gcagcacgag ggagccgacc gaggcgacgg gtgccccggt ggtgtccgcg 132600acggtgaggg tgacggcgtc gtcgcccgcg ggggtgatgc ggacccgggc ctcggtggcg 132660ccggccgcgt ggagggtgac gtcctgccag gcgaacggca ggccgccgcg ggcgtcctcg 132720ccgagcgggg cgagggcggt ggcgtgcagg gccgcgtcca gcagcgccgg gtgcaggccg 132780aacgaagagg cgtcgcccgc gggtcccgcg tcgagagcca cctcggcgaa ggtctcctcg 132840ccccggcgcc agacggcgcg caggccgcgg aaggcggggc cgtaggcgaa gccggactcg 132900gccagcccct cgtagaagcc gtcgaggccg acactctcgg caccggcggg cggccagacc 132960gccgcgtcga actcctcgcc gtggtgggcg ccgttggtca gggtgccggc ggcgtgctgg 133020gtccagggcc ggtcgtcgtc gccgtcgggg cgggagtaca gggtcagggc gcggcggccg 133080gtctcgtcgg ggctgccgat ccagacctgg acctggacgc cgccgtcctc gggcagggcg 133140agcggggcgg cgagggtgag ttcctcgacg cggtcgcagc cgacctcgtc gccggcgcgg 133200acggcgagtt cgaggaaggc ggtgccggcc agcagcacgg tgccgcgcac cgtgtggtcg 133260gcgagccagg gatgggtgcg caccgagaga cggccggtga ggagcaggcc gtcggagttg 133320gcgagggtga cggcggcggc gagcagcggg tggtgggcgg cgccgagccc cgcggaacgc 133380atgtcgccgg tgagccccgt gacgcccttg ggccagaagc ggcggtgctg gaaggcgtag 133440gtcggcaggt cggtgtggcc ggcgccggtc ccctcgaacc agcggtccca gcgcaccggg 133500acgccgcgca ggtggagtcc ggtggcggtc tcggtgaggg tggcggcctc ggggcggtcc 133560
ttgcggagcg cgggcagcac ggcggcgcgc tcgtgcccgg cggcgtccag gacggtccgg 133620gtgagggcgg cgagcgtggc gtcggggccc agttcgagga agagccccgt gccgtgtccg 133680gcgagccagg agactccgtc ggcgaagcgc acggcgtggc ggacgtgatc ggtccagtag 133740ccggcggagg tgagctgctc gacggtggcg ggggcgccgg tgaggttgga gacgaccggg 133800acggtggggg cctgcgggga gagtccggcg acgaccgcgc ggaactcctc cagcatcggg 133860tccatgagcg gtgagtggaa cgcgtgggag acccgcagcc tgcgggtgcg gcgcccgagg 133920gccgcgacgc gggcggcgat ggactcgacg gcgtcctcgg ctccggagac gacgaccgcg 133980gccgggccgt tgacggcggc gacggagacc tgtccggcgt gttcggcgac gagcggcgcg 134040acctcgtcct cggtggcttc cagggagacc atggcgccgc cctcgtccag cgcctgcatg 134100aggcggccac gggcggtgac cagggtgcag gcgtcggcga gggtgaacac cccggcgacg 134160tgggcggcgg ctatctcgcc gacggagtgg ccggtgaggt ggtcgggggt gacgccccag 134220gactcgacga gccggtacag ggccacttcg agggcgaaca gggcgggctg cgcgtactcg 134280gtgcggtcga ggagcgcggc ctcctcgctg ccctcctcgg cgaggatcag gggcttcagc 134340gggcggtcga gccccgcgtc gaggtgctcc aggatctcgt cgagggcgcg ggcgaagacg 134400gggaagcggg cgtgaaggtc gcggcccatg ccgggacgct gggagccctg gcccgagaag 134460agcacggcga gcttggtacg gccacgcagc tggtgggtgg tgagacccgg ggcgtccgca 134520ccggcggcga gcgcggtgag cacggcgacg gcggtgtccc ggtcggcggt ggtgaaggcc 134580gcgcggtgct ccagggcgga gcgggtggtg gcgagcgcgt gggagacgtc cagggcggac 134640ggcgtgggcc gggcggcgag gtggtcgagc agccgggcgg cctgggcgcg cagggcctcg 134700ggggtacggc cggagagcag ccagggcagc gccccggcgt cgacggccgg ggcggcctcg 134760ccgtcggcgg ccggctcctg gggagcctcc tcgatgatgg tgtgcgcgtt ggtgccggag 134820aggccgaagg aggagatgcc cgcgcgacgc ggcgcgttct cgcgggccgg ccagggggcg 134880ggctccgtca gcaggcggac ggagccctcg ctccagtcga cgtgggtgga gggctggtcg 134940acgtggaggg tgcgcggcag caggccgtgg cgcatcgcca tgaccatctt gatgacgccc 135000gcgacacccg ccgcggcctg ggtgtggctg atgttggact tgaccgagcc gagcagcaac 135060gggctgtcgg cctcgcggcc ctggccgtag gtggccagca gcgcctgggc ctcgacgggg 135120tcgccgagga ccgtgccggt gccgtgtgcc tcgacggcgt cgacgtcggc ggcggtgagc 135180cgggcgttga ccagggcgga ctcgatgacg cgctgctggg aggggccgtt gggggcggtc 135240aggccgttgg aggcaccgtc ctggttgacg gcggaccccc ggaccaccgc gaggacgcgg 135300tggccgttgc ggcgggcctc ggagagccgc tccaggacga ggacgccgac accctcggac 135360cagccggtgc cgttggcggc gtcggcgaag gaccggcagc gcccgtcggc ggcgaggccg 135420ccctggcggg cgaactcgac gaaggtggtg gggctggcca tgacggtgac accggcggcc 135480agggccaggg agcactcccc cgcgcgcagc gactgggcgg ccaggtgcag ggccaccagg 135540gaggaggagc aggcggtgtc gacggtcacg gccggtccga cggcaccgaa gaagtaggag 135600agccgcccgg agaggatgct ggtggcgttg ccggtgagct ggaagccctg gacgtcgtcg 135660tcggggccga cccggtagtc ctggggcatg gcgccgacga acatgccggt gcggctgccg 135720cgtacgcccg cggggtcgat accggcccgt tcgaaggcct cccagccggt ctccaggagc 135780aggcgctgct gcgggtccat ggacagcgcc tcgcggggcg agatgccgaa gaagtccggg 135840tcgaaggcgg gcgcgtcgtg caggaagccg ccggcgaagt cggcggggcc ctcgccgagc 135900gcggcctccc agccacggtc ctcgggcatg ccggtgatgc cgtcctcgcc ggcgaccagc 135960atcttccaca ggtcctcggg gctgctcacc ccgccggggt agcggcagct catgccgacg 136020atcgcgatgg gctcggtggc cgccgcctcc agctcggtca cccgctgccg ggagcgcttc 136080agatcggcgc tcgcccgctt caggtagtcc cggagcttct gttccttgtc catatcaacg 136140caacccatct ggatgaagac cggcggacgg ctggcggtcc ggcgtggaca ccgggtgccc 136200
gggacggccg gagggggccg ggccgggcgg ggtgtcccga ggaggggaaa gggcgggggg 136260cggggcctgg gcggcctgcc gggcgcggtg tctcacgtgc ctgcgggggc ggcccgggcg 136320ccgctccgcg gaccggagga ccagggcggc tctccggcac gcggacgggc gggcggcgcc 136380accgccgctc cccgggagca cacgccgagc acgcgtgccg tgccctcagt tccgctgacc 136440atggggccca gtctgcggcg ggtggggctt tggaaatccc tagtgtgtgc gcgggtgacg 136500gggccgcagg ttcgcaggtc acgagcggcc ccgccccggt ccccggacgg agccggccta 136560ccagctgccg gtggccagga gctggctgta gtcccgctcc cgcgatgcct ggcgcaggtc 136620ggactcgacc atcatgcgca tcagctgctc gaagtcgacc tcgggctccc agcccagttc 136680gcgccgggcg ttgcccgagt cggcgcagag ggtctcgacc tcggcggggc gcaccaggcc 136740ggggtcgacg acgacgtgct cctgccagtc gaggccgacg tgctcgaagg cgatccgggc 136800cgcgtcgcgg acctggtgca tggctccggt ccccaccacg tagtcaccgg cctgctcctg 136860ctggagcatc aggtgcatgg cgcggacgta gtcacccgcg aagccccagt cgcgctcggc 136920gtcgaggttg ccgaggtgca gcttgtccat ctggcccagt ttgatctggg cgacggccag 136980cgagatcttg cgggtgacga actcggcgcc acggcgcggc gactcgtggt tgaacaggat 137040cccggagacc ccgtacatcc cgtacgactc gcggtagttc tgggtgatgt agtggccgaa 137100ggccttggcc acgccgtacg ggctgcgggg gtggaaggag gtggtctccc gctgcgggct 137160ctccgccacc ttgccgtaca tctcggagga ggacgcctgg tagaagcgga tctggccgcg 137220gggcgagccg ccggaggact tggtgaggcc ggagacgatg cggaccgcct ccagcatgcg 137280cagcacgccg gtgccgttga cctccgtgac cagttcgggc tgttgccagg acatcggcac 137340gaaggagatg gcgcccaggt tgtagacctc gtcgggctgg accaggtcga ccgccgagac 137400caggctgccc tggtccatga ggtcgccgtc cacgaaggac agctccggga tcagcttggc 137460gatccggtcc ttgcgggggt tggcctgacc ccggcagagc ccccagacct ggtacccctg 137520gtcgaggagg tgttcggcca ggtacgaacc gtcctgtccg gtgattccgg tgatgagtgc 137580acgcttggac atctttcctc tttccaccgc cacggtgagg accggtcggg cgggtgtccg 137640cgccggcggg ggcggactga tggaccccca tgcgtactga cacgctccgg cggagggcag 137700agccttgccc ccctccggag cacgagacgc acggctgcgc tcacgagccg tcctcgtcgc 137760ggacatgcaa gcacgggcag agctaaggga tttgcgagtc ttcacggccg cgtcccgcgc 137820acgggccgac ggaaatcacc gcataacgcg gcacacccgc cgggttcagc cggcgccgcc 137880ggcctggcgg cgggagcgct ggtccttctc gtgggcgagc agtttcgcgt cgccctggag 137940gcgggcggcg cccttgcgga gtgcggggag cagctgcatg agggcgtcgc ccgggcagtt 138000ggtctcgtag gcgtcgatgt ggccgaagac ggccggcatg gtgacggtgg tgttcttggc 138060gaaccggctg tcgctgttcc tggagagcag gtgggtgttg gcgaccgggt cggcctgggt 138120gagggccagc ttccaggcga tgagtttctc gatggagcgg cgcatcggct cgggcacggg 138180ggtgccggcc gtgaaggtgc cga 138203<210>2<211>1743<212>PRT<213>鏈霉菌FR-008<400>2Met Val Pro Val His Ala His Asp Tyr Val Thr Asp Pro Pro Ser Thr1 5 10 15
Thr Gly Arg Thr Leu Asp Gly Leu Thr Leu Pro Arg Val Phe Ala Asp20 25 30Ala Val His Arg Gly Gly Asp Ala Val Ala Leu Val Asp Gly Glu Tyr35 40 45Ala Leu Thr Trp Ser Ala Trp Arg Thr Ala Val Asp Ala Leu Ala Arg50 55 60Gly Leu Gln Glu Ser Gly Val Val Ser Gly Asp Val Val Ala Leu His65 70 75 80Leu Pro Asn Ser Trp Glu Tyr Leu Thr Leu His Leu Ala Ala Ala Ser85 90 95Val Gly Ala Val Thr Met Pro Val His Gln Gly Asn Ala Pro Ser Asp100 105 110Val Arg Ala Leu Leu Glu Arg Val Arg Pro Ala Ala Val Val Leu Pro115 120 125Ala Arg Thr Gln Glu Gly Gly Gly Pro Leu Thr Gly Thr Ala Leu Arg130 135 140Glu Val Leu Pro Glu Leu Arg Ala Val Leu Val Thr Gly Asp Ala Ala145 150 155 160Gly Glu Gly Thr Glu Thr Val Thr Glu Met Leu Glu Arg Trp Ser Gly165 170 175Glu Asp Pro Leu Pro Val Glu Val Arg Pro Asp Ser Pro Phe Leu Leu180 185 190Leu Pro Ser Ser Gly Thr Thr Ser Ala Arg Pro Lys Ile Cys Leu His195 200 205Ser His Glu Gly Leu Leu Thr Asn Ser Arg Ala Ala Thr Glu Asp Thr210 215 220Ala Asp Ala Tyr Ala Gly Thr Leu Ile Thr Ala Cys Pro Leu Thr His225 230 235 240Cys Phe Gly Leu Gln Ser Ala Tyr Ser Ala Leu Phe Arg Ala Gly Arg245 250 255Gln Val Leu Leu Ser Gly Trp Asp Val Gly Arg Phe Leu Glu Leu Ala260 265 270Arg Arg Glu Arg Pro Ser Val Val Val Ala Val Pro Ala Gln Leu His275 280 285Asp Leu Val Thr Arg Val Arg Glu Asp Ala Asp Gly Pro Gly Phe Arg290 295 300Pro Gly Arg Ile Leu Thr Ala Gly Ala Ala Leu Pro Pro Ala Leu Val305 310 315 320Arg Asp Val Arg Glu Ala Leu Asp Thr Thr Leu Val Val Val Trp Gly325 330 335Met Ser Glu Ala Gly Asn Gly Thr Ser Ser Leu Ser Ala Asp Ala Pro340 345 350Glu Val Val Ser Arg Ser Val Gly Arg Pro Thr Arg Asp Ala Glu Met355 360 365
Arg Val Val Asp Glu Asp Gly Ala Pro Cys Pro Pro Gly Gln Pro Gly370 375 380Glu Leu Tyr Tyr Arg Ser Pro Ser Met Phe Arg Gly Tyr Phe Gly Glu385 390 395 400Pro Glu Leu Thr Arg Ser Val Val Ser Glu Asp Gly Trp Leu Arg Thr405 410 415Gly Asp Leu Ala Ser Ile Gly Glu Asp Gly Leu Val Thr Phe His Gly420 425 430Arg Ser Ala Glu Leu Ile Asn Val Gly Gly Arg Lys Phe Asn Ala Val435 440 445Glu Ile Gln Ala Leu Leu Ala Asp Leu Pro Asp Ile Gly Pro Leu Ala450 455 460Val Val Ala Ala Pro Asp Pro Arg Leu Gly Glu Tyr Pro Val Leu Val465 470 475 480Val Thr Glu Arg Pro Ala Ala Ala Pro Ala Asp Gly Thr Ala Pro Arg485 490 495Pro Arg Gly Thr Val Gly Leu Asp Glu Val Thr Ala His Leu Arg Gly500 505 510Leu Gly Thr Ala Glu Tyr Lys Ile Pro Leu Glu Leu Val Ala Leu Pro515 520 525Glu Leu Pro Arg Thr Pro Ala Gly Lys Ile Asn Arg Arg Ala Leu Glu530 535 540Gln Tyr Leu Ala Asp Ala Ala Glu Arg Thr Ala Val Thr Pro Ala Glu545 550 555 560Ala Pro Arg Pro Gly Leu Arg Thr Ala Leu Glu Leu Val Val Thr Ala565 570 575Val Ala Glu Val Leu Ala Ala Val Pro Gly Glu Asp Gly Ala Arg Pro580 585 590Ala Ala Ala Gly Pro Ile Gly Pro Asp Thr Thr Phe Arg Ala His Gly595 600 605Leu Asp Ser Val Ala Ser Val Arg Leu Arg Asn Ala Leu Ala Glu Ala610 615 620Thr Gly Leu Thr Leu Pro Ala Gly Leu Ala Phe Asp Phe Pro Thr Pro625 630 635 640Ala Ala Leu Ala Arg Glu Leu Ala Gly Leu Ser Ser Pro Ala Ala Glu645 650 655Glu Ser Pro Gly Ala Ser Ala His Glu Asp Glu Pro Val Ala Ile Val660 665 670Ser Met Ala Cys Arg Leu Pro Gly Gly Ala Thr Ser Pro Glu Ala Leu675 680 685Trp Glu Leu Leu Arg Asp Gly Val Asp Ala Val Ser Gly Phe Pro Glu690 695 700Asp Arg Gly Trp Asp Leu Asp Ala Leu Phe Gly Asp Asp Pro Asp Ala705 710 715 720
Pro Gly Thr Ser Val Ala Arg Glu Gly Gly Phe Leu Arg Asp Ala Ala725 730 735His Phe Asp Ala Gly Phe Phe Gly Met Ser Ala Arg Glu Ala Leu Ala740 745 750Thr Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ala Trp Glu Ala Val755 760 765Glu Arg Ala Gly Ile Ala Pro Arg Thr Leu Arg Gly Ser Arg Thr Gly770 775 780Val Phe Thr Gly Ala Met Tyr His Asp Tyr Ala Ala Gly Ala Ser Asp785 790 795 800Pro Ala Gly Glu Leu Glu Ser Leu Leu Pro Val Gly Thr Ala Gly Gly805 810 815Ala Leu Ser Gly Arg Ile Ala Tyr Thr Leu Gly Leu Ser Gly Pro Ala820 825 830Leu Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Leu835 840 845Ala Cys Arg Ser Leu Arg Ser Gly Glu Ser Asp Leu Ala Leu Ala Gly850 855 860Gly Val Ala Val Met Ala Thr Pro Ala Ala Phe Val Gly Phe Ser Arg865 870 875 880Leu Arg Gly Leu Ser Pro Asp Gly Arg Cys Lys Ser Phe Gly Glu Gly885 890 895Ala Asp Gly Ala Ala Trp Ser Glu Gly Ala Gly Leu Leu Met Leu Glu900 905 910Arg Leu Ser Asp Ala Arg Arg Asn Gly His Pro Val Leu Ala Val Ile915 920 925Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala930 935 940Pro His Gly Pro Ala Gln Arg Arg Val Val Arg Gln Ala Leu Ala Asp945 950 955 960Ala Gly Val Arg Ala Ala Glu Val Asp Val Val Glu Ala His Gly Thr965 970 975Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala Glu Ala Leu Leu Asp Thr980 985 990Tyr Gly Arg Asp Arg Pro Glu Gly Arg Pro Leu Trp Leu Gly Ser Val995 10001005Lys Ser Asn Leu Gly His Thr Gln Ala Ala Ala Gly Ala Ala Ala101010151020Val Ile Lys Met Val Leu Ala Leu Arg His Asp Leu Leu Pro Ala102510301035Thr Leu His Ala Asp Thr Pro Thr Ser Arg Val Asp Trp Ser Pro104010451050Gly Thr Val Gln Leu Leu Thr Arg Ala Arg Asp Trp Pro Arg Glu105510601065
Glu Gly Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Ile Ser107010751080Gly Thr Asn Ala His Leu Val Leu Glu Glu Ala Pro Val Pro Ala108510901095Ala Gly Thr Glu Arg Ser Ala Asp Ala Gly Ala Ala Gly Leu Arg110011051110Ala Ala Val Pro Trp Leu Val Ser Ala Lys Asp Ala Asp Ala Leu111511201125Arg Gly Gln Ala Arg Arg Leu Ala Ala His Ala Ala Ala His Pro113011351140Glu Val Ser Ala Arg Asp Leu Ala Tyr Ser Leu Leu Thr Thr Arg114511501155Ala Leu His Pro Arg Thr Ala Leu Leu Thr Gly Gly Asp Arg Asp116011651170Ala Leu Val Ala Ser Ala Asp Ala Phe Ala Arg Gly Glu Ala Pro1175Gly Ser Ile Val Arg Gly Pro Leu Gly Pro Ala Pro Gly Thr Ala119011951200Phe Val Leu Thr Gly Gln Gly Ser Gln Arg Leu Gly Met Gly Arg120512101215Gly Leu Ala Ala Ala Phe Pro Val Phe Asp Asp Ala Leu Arg Glu122012251230Val Cys Ala Leu Leu Asp Pro Leu Leu Glu Arg Pro Leu Thr Glu123512401245Val Met Trp Ala Ala Pro Asp Ser Asp Glu Ala Gly Leu Leu Gly125012551260Gly Thr Gly Tyr Ala Gln Pro Ala Leu Phe Ala Phe Glu Val Ala126512701275Leu Tyr Arg Leu Leu Glu Ser Trp Gly Ile Val Pro Asp Arg Leu128012851290Val Gly His Ser Val Gly Glu Ile Ala Ala Ala His Val Ala Gly129513001305Val Leu Ser Leu Pro Asp Ala Cys Ala Leu Val Ala Ala Arg Gly131013151320Arg Leu Met Gln Ala Leu Pro Pro Gly Gly Ala Met Ala Ala Val132513301335Arg Cys Ser Glu Ala Glu Ile Leu Pro Leu Leu Ala Gly Arg Thr134013451350Ala Gly Ala Thr Val Ala Ala Val Asn Gly Pro Arg Ser Val Val135513601365Leu Ser Gly Thr Glu Glu Ala Val Ala Glu Val Val Thr Glu Val137013751380Ser Ala Ala Gly His Lys Thr Arg Arg Leu Met Val Ser His Ala138513901395
Phe His Ser Pro Leu Met Glu Pro Met Leu Ala Glu Phe Arg Ala140014051410Thr Val Ala Gly Leu Ser Phe Ala Ala Pro Gln Val Pro Leu Val141514201425Ser Gly Val Thr Gly Arg Pro Leu Thr Ala Glu Glu Ala Arg Asp143014351440Pro Asp His Trp Val Arg His Ala Arg Asp Thr Val Arg Phe Ala144514501455Asp Ala Ile Ser His Leu Ala Gly Glu His Thr Glu Ile Tyr Val146014651470Glu Leu Gly Pro Glu Ala Ala Leu Thr Pro Met Val Glu Glu Cys147514801485Leu Gly Glu Pro Glu Ser Gly Asp Gly Pro Ala Val Glu Pro Val149014951500Val Arg Gly Asp Val Asp Glu Glu Arg Ala Ala Leu Ala Ala Ala150515101515Val Arg Leu His Ala Leu Gly Leu Asp Val Gln Trp Arg Ala Val152015251530Leu Pro Glu Ala Arg Ala Val Pro Leu Pro Thr Tyr Ala Phe Gln153515401545His Glu Ala Tyr Trp Leu Ala Thr Ser Gly Ser Val Val Ala Gly155015551560Leu Ser Leu Pro Gly Gly Arg Ala Ala Asp Thr Val Pro Asp Leu156515701575Ala Gly Arg Leu Ala Gly Leu Ser Gly Gly Glu Ala Glu Ala Leu158015851590Val Thr Glu Leu Val Arg Thr Glu Leu Ala Ala Val Thr Gly Gly159516001605Glu Ile Ser Ala Ala Gly Ala Gly Thr Ala Phe Thr Glu Leu Gly161016151620Val Thr Ser Val Thr Ala Val Glu Leu Arg Asn Arg Leu Thr Ala162516301635Val Thr Gly Val Arg Leu Pro Pro Thr Leu Ile Phe Asp His Pro164016451650Thr Pro Thr Ala Val Ala Arg Leu Ile Gly Glu Thr Val Arg Gly165516601665Ser Ser Val Pro Gly Arg Arg Asp Ala Val Ser Leu Val Asp Glu167016751680Leu Glu Ala Leu Leu Val Ser Gly Ala Glu Val Asp Ser Asp Thr168516901695Ala Ala Arg Leu Arg Ser Leu Ala Gly Arg Trp Ala Pro Ser Ala170017051710Thr Gly Thr Ala Ala Asp Ala Asn Gly Pro Leu Asp Leu Asp Asp171517201725
Ala Ser Asp Glu Glu Leu Phe Arg Leu Met Asp Gly Gly Ala Pro173017351740<210>3<211>10625<212>PRT<213>鏈霉菌FR-008<400>3Met Ala Ser Ser Glu Ala Lys Leu Arg Asp Tyr Leu Lys Lys Val Thr1 5 10 15Thr Asp Leu Arg Arg Thr Arg Gln Arg Leu Glu Thr Val Glu Ala Arg20 25 30Glu Ser Glu Pro Ile Ala Val Ile Gly Met Ala Cys Arg Phe Pro Gly35 40 45Gly Val Arg Ser Pro Glu Asp Leu Trp Gln Leu Val Ala Asp Gly Thr50 55 60Asp Ala Ile Gly Pro Leu Pro Ala Asp Arg Gly Trp Asp Leu Asp Gly65 70 75 80Leu Tyr Asp Pro Asp Pro Gly Thr Pro Gly Lys Ser Tyr Val Arg Glu85 90 95Gly Gly Phe Leu Glu Gly Ala Ala Asp Phe Asp Ala Asp Leu Phe Gly100 105 110Ile Ala Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu115 120 125Leu Glu Thr Ala Trp Glu Ala Val Glu Arg Ala Arg Ile Ser Pro Ala130 135 140Ser Leu Arg Asn Thr Asp Thr Gly Val Phe Val Gly Gly Ala Asp Thr145 150 155 160Asn Tyr Gly Ser Leu Ala Arg Thr Ala Glu Glu Thr Glu Gly His Asn165 170 175Leu Thr Gly Gly Ala Met Ser Val Leu Ser Gly Arg Ile Ser Tyr Thr180 185 190Leu Gly Leu Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser195 200 205Ser Leu Val Ala Leu His Leu Ala Val Arg Ala Leu Arg Ala Gly Glu210 215 220Cys Ser Leu Ala Leu Ala Gly Gly Val Ala Met Met Pro Thr Thr Glu225 230 235 240Leu Phe Thr Glu Phe Ser Arg Gln Arg Gly Leu Ala Thr Asp Gly Arg245 250 255Cys Lys Pro Phe Ala Glu Ala Ala Asp Gly Thr Ser Trp Gly Glu Gly260 265 270
Val Gly Val Leu Leu Val Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly275 280 285His Pro Val Leu Ala Val Val Arg Gly Thr Ala Val Asn Gln Asp Gly290 295 300Ala Ser Ser Arg Leu Thr Ala Pro Asn Gly Pro Ser Gln Arg Arg Val305 310 315 320Ile Glu Ala Ala Leu Ala Asp Ala Arg Leu Ala Ala Asp Gln Val Asp325 330 335Ala Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu340 345 350Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asn Arg Pro Glu Asp Arg355 360 365Pro Leu Arg Leu Gly Gly Ile Lys Ser Asn Ile Gly His Ala Gln Ala370 375 380Ala Ala Gly Val Ala Gly Val Ile Lys Met Val Met Ala Ile Arg His385 390 395 400Gly Leu Leu Pro Ala Thr Leu His Val Asp Thr Pro Thr Pro His Val405 410 415Asp Trp Thr Ala Gly Ala Val Glu Leu Leu Thr Glu Ala Val Asp Trp420 425 430Pro Asp Ala Asp Arg Pro Arg Arg Ala Gly Val Ser Ala Phe Gly Ile435 440 445Ser Gly Thr Asn Ala His Val Leu Leu Glu Gln Ala Thr Pro Glu Pro450 455 460Ala Gly Ala Glu Asp Thr Pro Gln Asp Ala Thr Pro Ala Glu Pro Val465 470 475 480Thr Ala Thr Asp Pro Ala Val Thr Pro Trp Leu Leu Ser Ala Arg Ser485 490 495Gln Thr Ala Leu Arg Ala Gln Ala Ala Arg Leu Leu Ala His Leu Thr500 505 510Asp His Thr Gly Pro Glu Ser Pro Arg Pro Ala Asp Ile Ala Leu Ser515 520 525Leu Ala Thr Ser Arg Ala Pro Leu Pro His Arg Ala Val Val Leu Ala530 535 540Thr Asp Pro Gly Thr Thr Glu Ala Ala Leu Thr Ser Leu Ala Ala Asp545 550 555 560Glu Pro His Pro Ala Val Leu Glu Asp Ala Val Arg Ser Ala Ser Thr565 570 575Ala Phe Leu Phe Ser Gly Gln Gly Ser Gln Arg Leu Gly Met Gly Arg580 585 590Gly Leu Tyr Glu Arg Phe Pro Val Phe Ala Glu Ala Phe Asp Ala Val595 600 605Cys Ala Gly Leu Asp Glu His Leu Glu Arg Pro Leu Arg Glu Val Val610 615 620
Trp Gly Asp Asp Thr Ser Val Leu Asp Gly Thr Ala Tyr Ala Gln Ala625 630 635 640Gly Leu Phe Ala Val Glu Val Ala Leu Phe Arg Leu Val Glu Ser Trp645 650 655Gly Val Arg Pro Glu Phe Val Ala Gly His Ser Ile Gly Glu Val Ala660 665 670Ala Ala His Val Ala Gly Val Phe Ser Leu Ala Asp Ala Cys Val Leu675 680 685Val Ala Ala Arg Gly Arg Leu Met Gln Ala Leu Pro Glu Gly Gly Ala690 695 700Met Val Ala Val Glu Ala Ser Glu Ala Glu Val Leu Pro Arg Leu Glu705 710 715 720Ser Val Glu Gly Val Ser Val Ala Ala Val Asn Gly Pro Ser Ser Val725 730 735Val Val Ser Gly Ala Glu Asp Ala Val Glu Ala Val Ala Glu Val Phe740 745 750Arg Glu Gln Gly Arg Arg Val Ser Arg Leu Arg Val Ser His Ala Phe755 760 765His Ser Pro Leu Met Glu Pro Met Leu Gly Asp Phe Arg Glu Ala Leu770 775 780Ala Gly Leu Ser Tyr Ala Glu Pro Ser Leu Pro Val Val Ser Asn Val785 790 795 800Ser Gly Arg Ile Ala Glu Pro Gly Glu Leu Thr Thr Pro Asp Tyr Trp805 810 815Val Arg His Val Arg Glu Ala Val Arg Phe Asp Asp Gly Val Gln Ala820 825 830Leu Ala Ala Glu Gly Val Ala Arg Phe Val Glu Leu Gly Pro Asp Gly835 840 845Val Leu Ser Gly Met Ala Arg Val Ser Ala Gly Glu Asp Ala Val Leu850 855 860Val Pro Leu Leu Arg Lys Asp Arg Asp Glu Glu Val Thr Ala Leu Ala865 870 875 880Ala Leu Gly Arg Leu His Ala Thr Gly Ala Pro Val Asp Trp Thr Ala885 890 895Val Leu Ala Gly Thr Gly Ala Arg Leu Val Asp Leu Pro Thr Tyr Ala900 905 910Phe Gln His Glu His Tyr Trp Pro Val Pro Ala Pro Ala Pro Ala Gly915 920 925Asp Val Glu Ser Ala Gly Leu Arg Ala Ala Gly His Pro Leu Leu Ser930 935 940Ala Ala Val Glu Leu Ser Asp Ser Gly Gly Leu Leu Leu Thr Thr Arg945 950 955 960Leu Ser Leu Arg Thr His Pro Trp Leu Gly Glu His Val Val Met Gly965 970 975
Asn Ala Leu Leu Pro Gly Thr Ala Phe Val Glu Leu Ala Val Arg Ala980 985 990Gly Asp Glu Val Gly Cys Asp Arg Val Glu Glu Leu Thr Leu Ala Ala995 10001005Pro Leu Val Leu Pro Glu Gln Gly Gly Val Gln Leu His Leu His101010151020Val Gly Pro Ala Asp Ala Ala Gly Arg Arg Val Leu Ala Ala Arg1025 10301035Ser Arg Ala Glu Gly Ala Asp Asp Gln Pro Trp Thr Glu His Ala104010451050Thr Gly Val Leu Ala Pro Gly Glu Arg Thr Val Glu Phe Asp Thr105510601065Ser Val Trp Pro Pro Ala Asp Ala Glu Ser Leu Asp Leu Thr Gly107010751080Leu Tyr Glu Arg Met Ala Glu Gly Gly Tyr Arg Tyr Gly Pro Leu108510901095Phe Gln Gly Leu Arg Ala Ala Trp Arg Arg Gly Asp Asp Val Phe110011051110Ala Glu Val Ala Leu Pro Asp Ser Ala Glu Arg Asp Ala Thr Ala111511201125Tyr Gly Leu His Pro Ala Leu Leu Asp Ala Ser Leu His Val Ser113011351140Ala Leu Gly Gly Leu Ala His Gly Val Val Pro Phe Ser Trp Glu114511501155Gly Val Cys Leu His Ala Thr Gly Ala Arg Glu Val Arg Val Arg116011651170Met Thr Arg Thr Gly Asp Glu Thr Val Thr Val Ala Val Ala Asp117511801185Pro Thr Gly Ala Pro Val Ala Ser Val Glu Asn Leu Val Leu Arg119011951200Arg Val Ala Pro Asp Ala Thr Gly Val Asp Ala Leu Thr Arg Asp120512101215Ala Leu Phe Arg Val Asp Trp Val Arg Thr Glu Ala Pro Gly Asp122012251230Ala Ala Gly Thr Gly Pro Val Gly Val Leu Gly Asp Ser Asp Ala123512401245Ser Thr Ala Leu Ala Gly Val Leu Arg Ala Asp Gly Leu Ser Asp125012551260Gly Gly Phe Val Ala Phe Pro Asp Leu Glu Asp Leu Ala Ala Ala126512701275Glu Glu Val Pro Ala Thr Val Leu Val Arg Val Arg Pro Asp Ala128012851290Ala Thr Arg Pro Asp Ala Val Val Asp Ala Val His Ala Leu Ala129513001305
Ala Arg Gly Leu Glu Leu Val Arg Glu Trp Leu Ala Gln Glu Arg131013151320Phe Thr Asp Ser Arg Leu Val Phe Val Thr Ser Asp Ala Val Asp132513301335Gly Gly Asp Leu Ala Asp Ala Ala Leu Arg Gly Leu Val Arg Ser134013451350Ala Val Ser Glu His Pro Gly Arg Phe Gly Leu Met Glu Val Gly135513601365Arg Gly Thr Asp Thr Ala Thr Val Leu Ala Ala Val Ala Thr Gly137013751380Glu Pro Glu Thr Ala Ala Arg Asp Gly Glu Val Leu Ala Pro Arg138513901395Leu Ala Arg Ile Thr Ser Gly Thr Thr Thr Gly Pro Arg Trp Asp140014051410Ser Ile Asp Gly Thr Val Leu Leu Thr Gly Gly Thr Gly Gly Leu141514201425Gly Arg Ile Val Ala Arg His Leu Val Val Glu Arg Gly Val Arg143014351440Asp Leu Leu Leu Val Ser Arg Ser Gly Ala Ala Ala Agp Gly Ala144514501455Gly Gln Leu Val Ala Glu Leu Ser Glu Thr Gly Ala His Val Thr146014651470Val Glu Ala Cys Asp Val Ser Asp Pro Ala Ala Val Ala Glu Leu147514801485Val Ala Arg His Pro Val Arg Ala Val Val His Ser Ala Gly Val149014951500Leu Asp Asp Gly Thr Val Glu Ser Leu Thr Ala Glu Arg Val Thr150515101515Arg Val Leu Arg Pro Lys Ala Asp Ala Ala Trp Asn Leu His Glu152015251530Ala Thr Arg Ala Leu Asp Leu Glu Ala Phe Val Val Phe Ser Ser153515401545Met Ser Gly Ile Leu Gly Gly Pro Gly Gln Ala Asn Tyr Ala Ala155015551560Gly Asn Thr Phe Leu Asp Ala Leu Val Arg His Arg Arg Ser Leu156515701575Gly Leu Pro Ala Thr Ser Leu Ala Trp Gly Pro Trp Thr Gln Asp158015851590Ala Gly Met Ile Gly Thr Leu Ser Gly Thr Ser Arg His Arg Val159516001605Ala Arg Ala Gly Met Pro Glu Leu Thr Ala Glu Gln Gly Thr Ala161016151620Leu Phe Asp Ala Ala Leu Ala Ser Gly Glu Pro Val Val Leu Pro162516301635
Val Arg Leu Asp Leu Ala Ala Leu Arg Gly Gln Gly Asp Leu His164016451650Pro Met Met Leu Gly Leu Val Arg Arg Arg Gly Arg Arg Ala Ala165516601665Ala Gly Gly Ser Val Ala Ala Ala Gly Leu Val Gln Arg Leu Gly167016751680Ala Leu Gly Val Asp Glu Arg Arg Glu Val Leu Leu Asp Leu Val168516901695Arg Gly Gln Val Ala Val Val Leu Gly His Ala Gly Val Gln Ser170017051710Val Asp Pro Gly Arg Ala Phe Gln Asp Leu Gly Phe Asp Ser Leu171517201725Thr Ala Val Glu Leu Arg Asn Arg Leu Gly Lys Ser Thr Gly Leu173017351740Arg Leu Pro Ala Thr Val Val Phe Asp Tyr Pro Thr Val Asn Ala174517501755Leu Val Gly Tyr Leu Leu Glu Glu Leu Phe Gly Gly Val Glu Pro176017651770Ala Ser Val Val Pro Val Ser Ala Leu Pro Ser Val Ala Glu Asp177517801785Pro Ile Val Ile Val Gly Met Ala Cys Arg Tyr Pro Gly Gly Val179017951800Ser Ser Pro Glu Asp Leu Trp Thr Val Val Ser Glu Gly Val Asp180518101815Thr Val Ser Glu Phe Pro Val Asn Arg Gly Trp Asp Val Asp Ala182018251830Leu Tyr Asn Pro Asp Arg Asp Ala Ser Gly Thr Thr Tyr Thr Lys183518401845Ala Gly Gly Phe Leu His Asp Ala Gly Ala Phe Asp Ser Glu Phe185018551860Phe Gly Met Ser Pro Arg Glu Ala Val Ala Thr Asp Ser Gln Gln186518701875Arg Leu Leu Leu Glu Thr Thr Trp Glu Ala Ile Glu Arg Ser Gly188018851890Ile Asp Pro Ala Ser Leu Arg Gly Ser Gln Thr Gly Val Phe Ala189519001905Gly Val Met Tyr Asn Asp Tyr Gly Asn Met Leu Ala Asp Glu Arg191019151920Tyr Glu Gly Phe Arg Ser Asn Gly Ser Ala Pro Ser Ile Ala Ser192519301935Gly Arg Val Ser Tyr Thr Phe Gly Phe Glu Gly Pro Ala Val Thr194019451950Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Trp Ala195519601965
Ala Gln Ala Leu Arg Ser Gly Glu Cys Ser Leu Ala Val Ala Gly197019751980Gly Val Thr Val Met Ser Thr Pro Thr Thr Phe Val Glu Phe Ser198519901995Arg Gln Gly Gly Leu Ser Ala Asp Gly Arg Cys Arg Ser Phe Ala200020052010Asp Ser Ala Asp Gly Val Gly Trp Ser Glu Gly Val Gly Met Val201520202025Val Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val203020352040Leu Ala Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser204520502055Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile206020652070Arg Gln Ala Leu Ala Ser Gly Gly Leu Ser Ala Gly Asp Val Asp207520802085Val Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile209020952100Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Glu Arg Glu Glu210521102115Gly Arg Pro Leu Trp Leu Gly Ser Val Lys Ser Asn Leu Gly His212021252130Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val Met213521402145Ala Met Arg His Gly Arg Leu Pro Arg Thr Leu His Val Asp Ala215021552160Pro Ser Ser His Val Asp Trp Ser Ala Gly Ala Val Glu Leu Leu216521702175Thr Gly Glu Arg Val Trp Pro Gly Glu Glu Arg Leu Arg Arg Ala218021852190Gly Val Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His Val Ile219522002205Leu Glu Gln Pro Glu Pro Val Ala Glu Pro Glu Thr Gly Gly Asp221022152220Gly Ile Ala Ala Leu Ala Pro Gly Ile Val Pro Trp Val Leu Ser222522302235Gly Arg Thr Glu Glu Ala Leu Arg Ala Gln Ala Ala Arg Leu Leu224022452250Ala Gln Val Glu Gly His Pro Gly Leu Arg Pro Val Asp Leu Ala225522602265Leu Ser Leu Ala Thr Gln Arg Ser Leu Phe Asp His Arg Ala Val227022752280Val Leu Thr Asp Asp Arg Glu Thr Ala Val Arg Gly Leu Ala Ala228522902295
Val Cys Val Gly Glu Pro Asp Pro Ala Val Val Leu Gly Ala Val230023052310Glu Pro Gly Arg Thr Ala Phe Leu Phe Ser Gly Gln Gly Ser Gln231523202325Arg Leu Gly Met Gly Arg Gly Leu Tyr Glu Arg Phe Pro Val Phe233023352340Ala Glu Ala Phe Asp Ala Val Cys Ala Gly Leu Asp Ala His Leu234523502355Asp Arg Pro Leu Arg Glu Val Val Trp Gly Asp Asp Thr Ser Val236023652370Leu Asp Gly Thr Ala Tyr Ala Gln Ala Gly Leu Phe Ala Val Glu237523802385Val Ala Leu Phe Arg Leu Val Glu Ser Trp Gly Val Arg Pro Glu239023952400Phe Val Ala Gly His Ser Ile Gly Glu Val Ala Ala Ala His Val240524102415Ala Gly Val Phe Ser Leu Ala Asp Ala Cys Val Leu Val Ala Ala242024252430Arg Gly Arg Leu Met Gln Ala Leu Pro Glu Gly Gly Ala Met Val243524402445Ala Val Glu Ala Ser Glu Ala Glu Val Leu Pro Arg Leu Glu Ser245024552460Val Glu Gly Val Ser Val Ala Ala Val Asn Gly Pro Ser Ser Val246524702475Val Val Ser Gly Ala Glu Glu Ala Val Glu Ala Val Ala Glu Val248024852490Phe Arg Glu Leu Gly Arg Arg Val Ser Arg Leu Arg Val Ser His249525002505Ala Phe His Ser Pro Leu Met Glu Pro Met Leu Asp Ala Phe His251025152520Glu Val Leu Arg Asp Leu Ser Phe Ala Glu Pro Arg Leu Pro Val252525302535Val Ser Asn Val Ser Gly Arg Ile Ala Glu Pro Gly Glu Leu Thr254025452550Ala Pro Asp Tyr Trp Val Arg His Val Arg Glu Ala Val Arg Phe255525602565Asp Asp Gly Val Arg Ala Leu Ala Glu Glu Gly Val Thr Arg Phe257025752580Val Glu Leu Gly Pro Asp Gly Val Leu Ser Gly Met Ala Arg Ala258525902595Ser Ala Gly Glu Asp Ala Val Leu Val Pro Leu Leu Arg Lys Asp260026052610Arg Glu Glu Val Ala Val Ala Leu Ser Ala Leu Ala Glu Leu His261526202625
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Val Ile Leu Glu Gln Pro Glu Pro Val Thr Gly Ser Gly Pro Gly395039553960Glu Ala Asp Gly Thr Glu Ala Ser Ser Gly Val Gln Pro Trp Val396539703975Leu Ser Ala Arg Thr Glu Glu Ala Leu Arg Ala Gln Ala Ala Arg398039853990Leu Ala Asp Phe Leu Thr Asp Ala Pro Ala Asp Val Arg Asp Val399540004005Ala Leu Ser Leu Ala Thr Gln Arg Thr Leu Phe Asp His Arg Ala401040154020Val Val Leu Gly Ala Asp Leu Glu Thr Ala Leu Thr Ser Leu Arg402540304035Ala Leu Ala Ala Gly Ala Pro Asp Ala Ala Val Ala Glu Gly Val404040454050Ala Gly Asn Gly Arg Thr Ala Phe Leu Phe Pro Gly Gln Gly Ser405540604065Gln Arg Leu Gly Met Gly Arg Asp Leu Tyr Glu Arg Phe Pro Val407040754080Leu Ala Glu Ala Phe Asp Ala Val Cys Ala Glu Leu Asp Glu His408540904095Leu Asp Arg Pro Leu Arg Glu Val Val Trp Gly Asp Asp Ala Glu410041054110Leu Leu Asn Arg Thr Ala Tyr Ala Gln Pro Gly Leu Phe Ala Val411541204125Glu Val Ala Leu Phe Arg Leu Val Glu Ser Trp Gly Val Arg Pro413041354140Glu Phe Val Ala Gly His Ser Ile Gly Glu Val Ala Ala Ala His414541504155Val Ala Gly Val Phe Thr Leu Ala Asp Ala Ala Ala Leu Val Ala416041654170Ala Arg Gly Arg Leu Met Gln Ala Leu Pro Glu Gly Gly Ala Met417541804185Ala Ala Val Glu Ala Thr Cys Ala Glu Val Leu Pro His Leu Thr419041954200Gly Asn Leu Ser Ile Ala Ala Val Asn Gly Pro Ser Ser Val Val420542104215Val Ser Gly Ser Glu Ala Ser Val Glu Ser Val Ala Glu Val Phe422042254230Arg Glu Leu Gly Arg Arg Val Ser Arg Leu Arg Val Ser His Ala423542404245Phe His Ser Pro Leu Met Glu Pro Met Leu His Ala Phe Arg Glu425042554260Val Val Glu Gly Leu Ser Phe Ala Ala Pro Arg Ile Pro Leu Val426542704275
Ser Asn Ile Thr Gly Thr Leu Ala Glu Pro Gly Gln Val Asp Glu428042854290Pro Glu Tyr Trp Val Thr His Val Arg Glu Ala Val Arg Phe Asp429543004305Asp Gly Val Arg Ala Leu Ala Glu Glu Gly Val Thr Arg Phe Val431043154320Glu Val Gly Pro Asp Gly Val Leu Ser Gly Met Ala Arg Glu Ser432543304335Ala Gly Glu Asp Ala Val Leu Val Pro Leu Leu Arg Lys Asp Arg434043454350Glu Glu Thr Gly Ser Ala Leu Ser Ala Leu Gly Arg Leu His Thr435543604365Val Gly Val Asp Val Asp Trp Ser Gly Phe Leu Val Gly Ala Arg437043754380Pro Val Asp Leu Pro Thr Tyr Ala Phe Gln Lys Gln Arg Tyr Trp438543904395Pro Glu Ala Thr Leu Pro Arg Ala Gly Asp Val Arg Phe Ala Gly440044054410Leu Gly Ser Ala Gly His Pro Leu Leu Gly Ala Ala Val Glu Leu441544204425Ala Gly Ala Asp Gly Ala Asp Gly Val Val Leu Thr Gly Arg Leu443044354440Ser Thr Arg Ser His Pro Trp Leu Ala Asp His Val Val Gln Gly444544504455Thr Val Leu Val Pro Gly Thr Ala Leu Leu Glu Met Ala Val Arg446044654470Ala Ala Asp Glu Ala Gly Cys Gly Ser Val Glu Glu Leu Thr Leu447544804485Ser Ala Pro Leu Val Leu Pro Glu Arg Gly Ala Leu Gln Ile Gln449044954500Ile Arg Val Ala Ala Pro Asp Glu Asp Gly Arg Arg Ala Leu Gly450545104515Val His Ala Arg Thr Glu Asp Asp Asp Gly Ala Pro Trp Thr Val452045254530His Ala Thr Gly Thr Leu Ala Pro Glu Thr Leu Ala Pro Asp Pro453545404545Phe Asp Ala Thr Val Trp Pro Pro Arg Asp Ala Ala Gln Val Asp455045554560Val Thr Asp Cys Tyr Glu Arg Leu Ala Glu Ala Gly Phe Ala Tyr456545704575Gly Pro Ala Phe Arg Gly Leu Arg Ala Ala Trp Arg His Gly Asp458045854590Ala Leu Tyr Ala Glu Val Ala Leu Asp Gly Gly Thr Asp Gly Asp459546004605
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Val Thr Ser Pro Glu Asp Leu Trp Ser Val Val Ser Asp Gly Val527052755280Asp Ala Val Gly Asp Phe Pro Ser Asp Arg Gly Trp Asp Val Glu528552905295Ser Leu Tyr Ser Glu Asp Arg Gly Val Pro Gly Thr Thr Tyr Thr530053055310Arg Ser Gly Gly Phe Leu His Asp Ala Pro Glu Phe Asp Pro Glu531553205325Phe Phe Gly Met Ser Pro Arg Glu Ala Val Ser Thr Asp Ala Gln533053355340Gln Arg Leu Leu Leu Glu Thr Thr Trp Glu Ala Ile Glu Arg Ser534553505355Gly Ile Asp Pro Val Ser Leu Arg Gly Ser Gln Thr Gly Val Phe536053655370Ala Gly Val Met Tyr Asn Asp Tyr Gly Ser Ile Leu Thr Asp Asp537553805385Gln Tyr Glu Gly Tyr Arg Gly Asn Gly Ser Ala Gly Ser Ile Ala539053955400Ser Gly Arg Val Ser Tyr Thr Phe Gly Phe Glu Gly Pro Ala Val540554105415Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Met His Trp542054255430Ala Ala Gln Ala Leu Arg Ser Gly Glu Cys Ser Leu Ala Val Ala543554405445Gly Gly Val Thr Val Met Ala Thr Pro Thr Ala Phe Val Glu Phe545054555460Ser Arg Gln Gly Ala Leu Ser Pro Asp Ser Arg Cys Lys Ala Phe546554705475Ser Asp Ala Ala Asp Gly Ala Gly Trp Ser Glu Gly Val Gly Met548054855490Val Val Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg549555005505Val Leu Ala Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala551055155520Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val552555305535Ile Arg Gln Ala Leu Ala Ser Gly Gly Leu Ser Ala Gly Asp Val554055455550Asp Val Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro555555605565Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Gly Arg Asp557055755580Glu Glu Arg Pro Leu Leu Leu Gly Ser Val Lys Ser Asn Ile Gly558555905595
His Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val560056055610Met Ala Met Arg His Gly Arg Leu Pro Arg Thr Leu His Val Asp561556205625Ala Pro Ser Ser His Val Asp Trp Ser Ala Gly Ala Val Glu Leu563056355640Leu Thr Gly Glu Arg Val Trp Pro Gly Glu Glu Arg Leu Arg Arg564556505655Ala Gly Val Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His Val566056655670Ile Leu Glu Gln Pro Glu Glu Ala Gln Glu Pro Ala Pro Thr Val567556805685Ala Pro Ala Val Asp Val Pro Trp Val Leu Ser Ala Arg Ser Ala569056955700Pro Ala Leu Arg Ala Gln Ala Glu Arg Leu Arg Val His Val Ala570557105715Ala Glu Pro Val Ser Ala Pro Asp Val Ala Phe Ser Leu Val Ser572057255730Gly Arg Ala Thr Phe Asp His Arg Ala Val Val Leu Gly Thr Asp573557405745Arg Glu Ala Ala Leu Glu Ala Leu Ala Ser Gly Leu Pro Asp Ala575057555760Gly Val Val Glu Gly Val Ala Thr Gly Gly Arg Thr Ala Phe Leu576557705775Phe Ser Gly Gln Gly Ser Gln Arg Leu Gly Met Gly Arg Gly Leu578057855790Tyr Glu Arg Phe Pro Val Phe Ala Glu Ala Phe Asp Ala Val Cys579558005805Ala Gly Leu Asp Glu His Leu Glu Arg Pro Leu Arg Glu Val Val581058155820Trp Gly Asp Asp Thr Ser Val Leu Asp Gly Thr Ala Tyr Ala Gln582558305835Ala Gly Leu Phe Ala Val Glu Val Ala Leu Phe Arg Leu Val Glu584058455850Ser Trp Gly Val Arg Pro Glu Phe Val Ala Gly His Ser Ile Gly585558605865Glu Val Ala Ala Ala His Val Ala Gly Val Phe Thr Leu Ala Asp587058755880Ala Ala Ala Leu Val Ala Ala Arg Gly Arg Leu Met Gln Ala Leu588558905895Pro Glu Gly Gly Ala Met Val Ala Leu Glu Ala Ser Glu Ala Glu590059055910Val Leu Pro Arg Leu Glu Ser Val Glu Gly Val Ser Val Ala Ala591559205925
Val Asn Gly Ser Ser Ser Val Val Val Ser Gly Ala Glu Asp Ala593059355940Val Glu Ala Val Ala Glu Val Phe Arg Glu Gln Gly Arg Arg Val594559505955Ser Arg Leu Arg Val Ser His Ala Phe His Ser Pro Leu Met Glu596059655970Pro Met Leu Gly Asp Phe Arg Glu Val Leu Ser Gly Leu Ser Tyr597559805985Ala Glu Pro Ser Leu Pro Val Val Ser Asn Val Ser Gly Arg Ile599059956000Ala Glu Pro Gly Glu Leu Thr Thr Pro Asp Tyr Trp Val Thr His600560106015Val Arg Glu Ala Val Arg Phe Asp Asp Gly Val Arg Ala Leu Ala602060256030Glu Glu Gly Val Thr Arg Phe Val Glu Leu Gly Pro Asp Gly Val603560406045Leu Ser Gly Met Ala Arg Glu Gly Ala Gly Glu Asp Ala Val Leu605060556060Val Pro Leu Leu Arg Lys Asp Arg Glu Glu Thr Ser Thr Ala Leu606560706075Ala Ala Leu Ala Arg Leu His Thr Val Gly Ala Glu Val Lys Trp608060856090Thr Gly Phe Phe Ala Gly Thr Ala Ala Arg Thr Val Asp Leu Pro609561006105Thr Tyr Ala Phe Gln Lys Arg Arg Phe Trp Pro Glu Thr Thr Val611061156120Arg Ala Ala Ala Asp Pro Arg Ser Ala Gly Val Asp Ala Ala Asp612561306135His Pro Leu Leu Gly Ala Val Val Ser Leu Pro Asp Ser Gly Gly614061456150Val Val Leu Thr Gly Arg Leu Ser Val Glu Ala Gln Pro Trp Leu615561606165Ala Asp His Val Val Leu Gly Arg Val Leu Leu Pro Gly Thr Gly617061756180Leu Val Glu Leu Ala Leu Ala Ala Gly Asp Ala Ala Gly Ser Thr618561906195Ala Leu Glu Glu Leu Thr Leu Ala Ala Pro Leu Val Leu Pro Glu620062056210Asp Thr Gly Leu Gln Leu Arg Val Val Ala Gly Pro Lys Thr Asp621562206225Gly Arg Arg Thr Val Ala Val His Ser Arg Pro Glu Gly Ala Glu623062356240Asp Ala Pro Trp Thr Ala His Ala His Gly Phe Leu Thr Asp Ala624562506255
Pro Ala Gly Ser Gly Glu Ala Leu Thr Glu Trp Pro Pro Pro Gly626062656270Ala Asp Pro Val Pro Val Glu His Ala Tyr Glu Glu Phe Arg His627562806285Arg Gly Tyr Gly Tyr Gly Pro Val Phe Gln Gly Leu Arg Ala Ala629062956300Trp Arg Arg Asp Asp Ala Met Phe Ala Glu Val Ala Leu Pro Glu630563106315Asp Ala Ala Gly Glu Ala Gly Arg Phe Gly Leu His Pro Ala Val632063256330Leu Asp Ala Ala Met His Ala Gly Ile Leu Asn Glu Glu Glu Gly633563406345Gln Ala Val Val Pro Phe Ala Trp Asn Asp Val Thr Leu His Ala635063556360Val Gly Ala Ser Ala Val Arg Val Arg Val Ser Arg Val Asp Ala636563706375His Thr Val Ser Leu Thr Leu Ala Asp Ser Thr Gly Ala Pro Val638063856390Leu Thr Val Gly Ser Leu Ala Ser Arg Pro Val Ser Ala Glu Gln639564006405Leu Gly Ser Ala Ser Ala Gly Ala Gly Ala Leu Tyr Gly Ile Glu641064156420Trp Thr Pro Val Thr Val Asp Thr Ser Thr Ala Pro Ala His Val642564306435Ser Trp Ala Glu Ala Leu Glu Ala Asp Thr Thr Ala Pro Gly Ala644064456450Val Val Leu Glu Val Arg Glu Pro Ala Gly Pro Asp Val Pro Ala645564606465Ala Val Arg Ala Val Leu Asp Gln Val Leu Pro Ala Ile Gln Gln647064756480Trp Leu Arg Asp Glu Arg Phe Thr Ser Ser Arg Leu Val Val Val648564906495Thr Arg Gly Ala Ala Pro Ala Gly Ser Ser Ala Asp Val Val Gln650065056510Ala Pro Val Trp Gly Leu Val Arg Ala Ala Leu Ala Glu Asn Pro651565206525Gly Arg Phe Ala Leu Ala Asp Val Ser Val Asp Ala Asp Asp Ala653065356540Ala Val Asp Arg Ala Val Ala Ala Ala Leu Ser Gly Glu Ala Glu654565506555Val Ala Val Arg Asp Gly Val Val Leu Val Pro Arg Leu Ser Arg656065656570Leu Ala Glu Pro Glu Leu Pro Leu Ser Val Pro Ser Leu Asp Gly657565806585
Glu Gly Ala Val Leu Val Thr Gly Gly Thr Gly Gly Leu Gly Ala659065956600Val Met Ala Arg Tyr Leu Val Ala Glu Arg Gly Val Arg Asp Leu660566106615Val Leu Val Ser Arg Arg Gly Gly Asp Ala Pro Gly Ala Ala Glu662066256630Leu Ala Gly Glu Leu Arg Glu Ala Gly Ala Ala Val Glu Val Val663566406645Ala Cys Asp Leu Ser Asp Arg Glu Ser Val Val Gly Leu Val Glu665066556660Ser Leu Val Ala Gly Arg Gly Leu Arg Ala Val Val His Ala Ala666566706675Gly Val Gly Gly Gly Gly Leu Val Gly Thr Leu Ser Ser Asp Arg668066856690Phe Asp Ala Val Leu Gly Ala Lys Ala Asp Ala Ala Trp Trp Leu669567006705His Glu Ala Thr Ala Gly Val Glu Leu Ala Ala Phe Val Leu Val671067156720Ser Ser Ala Gly Gly Leu Val Leu Thr Ala Gly Gln Gly Asn Tyr672567306735Ala Ala Ala Asn Val Phe Leu Asp Ala Leu Ala Ser Arg Arg Arg674067456750Ala Glu Gly Leu Val Ala Thr Ser Met Ala Phe Gly Phe Trp Asp675567606765Val Gly Ala Gly Leu Gly Glu Tyr Leu Ser Glu Val Asp Arg Arg677067756780Arg Met Ala Ser Gln Gly Leu Pro Leu Leu Ser His Glu Ala Gly678567906795Leu Glu Leu Phe Ala Ala Gly Leu Asp Arg Gly Glu Ala Thr Val680068056810Val Pro Leu Arg Val Asp Thr Ala Ala Leu Arg Thr Arg Thr Asp681568206825Glu Ile Pro Ala Leu Leu Lys Ser Leu Ala Pro Val Arg Arg Ser683068356840Ala Ala Val Val Pro Pro Ala Thr Val Glu Gly Ser Pro Ala His684568506855Arg Leu Ala Ser Leu Pro Glu Ala Glu Arg His Arg Thr Leu Leu686068656870His Leu Val Arg Ser Gln Val Ala Ala Val Leu Gly His Gly Ser687568806885Ala Glu Ala Ile Gly Ala Asp Arg Ala Phe Gln Glu Leu Gly Phe689068956900Asp Ser Leu Ala Ala Thr Glu Leu Arg Asn Gln Leu Asn Thr Leu690569106915
Thr Gly Leu Arg Leu Pro Ala Thr Leu Val Phe Asp His Pro Asn692069256930Ala Leu Ala Val Thr Asp His Leu Ser Ala Arg Leu Ala Gly Glu693569406945Ser Ala Arg Ala Glu Thr Pro Val Arg Thr Gly Thr Ala Ala Pro695069556960Ala Asp Asp Glu Pro Ile Ala Ile Val Gly Met Ala Cys Arg Tyr696569706975Pro Gly Gly Val Thr Thr Pro Glu Glu Leu Trp Arg Leu Val Thr698069856990Asp Gly Val Asp Thr Val Ser Asp Leu Pro Gly Asp Arg Gly Trp699570007005Asp Ile Glu Gly Leu Tyr Asp Pro Glu Pro Gly Lys Glu Gly Lys701070157020Ser Tyr Thr Arg Arg Gly Ser Phe Leu His Asp Ala Ala Gln Phe702570307035Asp Pro Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Tyr Met704070457050Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala Leu705570607065Glu Arg Ala Gly Ile Asp Pro Ala Thr Leu Arg Gly Ser Arg Thr707070757080Gly Val Phe Ala Gly Val Met Tyr His Asp Tyr Ala Leu Asn Val708570907095Ser Pro Ser Gly Thr Ala Gly Gly Ser Val Val Ser Gly Arg Leu710071057110Ser Tyr Thr Phe Gly Trp Glu Gly Pro Ala Val Thr Val Asp Thr711571207125Ala Cys Ser Ser Ser Leu Val Ala Leu His Leu Ala Val Gln Ala713071357140Leu Arg Ser Gly Glu Cys Ser Leu Ala Val Ala Gly Gly Ala Thr714571507155Val Met Ser Thr Pro Gly Met Phe Val Glu Phe Ser Arg Gln Arg716071657170Gly Leu Ser Val Asp Gly Arg Cys Lys Ala Phe Ala Gly Ala Ala717571807185Asp Gly Val Gly Trp Ser Glu Gly Val Gly Val Leu Leu Val Glu719071957200Arg Leu Ser Asp Ala Val Arg Asn Gly His Arg Val Leu Ala Val720572107215Val Lys Gly Thr Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Phe722072257230Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala723572407245
Leu His Gly Ala Gly Val Pro Ala Ser Glu Val Asp Val Val Glu725072557260Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln726572707275Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg Pro Glu Asp Arg Pro728072857290Leu Leu Leu Gly Ser Val Lys Ser Asn Ile Gly His Ala Gln Ala729573007305Ala Ala Gly Val Ala Gly Val Ile Lys Met Val Met Ala Met Glu731073157320His Gly Thr Val Pro Arg Thr Leu His Val Asp Arg Pro Ser Pro732573307335His Val Asp Trp Thr Glu Gly Arg Ala Glu Leu Val Thr Glu Asp734073457350Arg Pro Trp Pro Val Thr Gly Arg Pro Arg Arg Ala Ser Val Ser735573607365Ala Phe Gly Leu Ser Gly Thr Asn Ala His Val Val Leu Glu Gln737073757380Gly Pro Asp Ala Ala Ala Asp Ala Pro Ser Thr Ala Glu Glu Gly738573907395Arg Ala Pro Ala Val Leu Pro Trp Thr Val Ser Ala Ala Thr Pro740074057410Asp Ala Leu Arg Ala Gln Ala Thr Arg Leu Leu Asp His Leu Ala741574207425Asp Arg Pro Gly Thr Arg Pro Leu Asp Val Ala His Ala Leu Ala743074357440Thr Ser Arg Thr Pro Leu Glu Ala Arg Ala Val Val Leu Gly Thr744574507455Gly Arg Asp Asp Leu Leu Thr Gly Leu Arg Ala Leu Ala Ala Gly746074657470Glu His Ala Pro Gly Val Ile Thr Gly Thr Ala Arg Ser Val Gly747574807485Thr Thr Ala Phe Leu Phe Ser Gly Gln Gly Ala Gln Arg Leu Glu749074957500Met Gly Arg Gly Leu His Glu Ala Phe Pro Val Phe Ala Glu Ala750575107515Phe Asp Ala Val Cys Ala Gly Leu Asp Glu His Leu Asp Arg Pro752075257530Leu Arg Glu Val Ala Trp Gly Glu Asn Ala Pro Asp Leu Asp Gly753575407545Thr Ala Tyr Ala Gln Ser Ala Leu Phe Ala Tyr Glu Val Ala Leu755075557560Phe Arg Leu Leu Ala Ser Trp Gly Val Thr Pro Asp Leu Val Ala756575707575
Gly His Ser Ile Gly Glu Val Ala Ala Ala His Val Ala Gly Val758075857590Phe Ser Leu Ala Asp Ala Cys Ala Leu Val Ala Ala Arg Gly Arg759576007605Leu Met Gln Ala Leu Pro Glu Gly Gly Ala Met Val Ala Val Glu761076157620Ala Ser Glu Thr Glu Val Leu Pro His Leu Glu Ser Val Glu Gly762576307635Val Ser Val Ala Ala Val Asn Gly Pro Ser Ser Val Val Val Ser764076457650Gly Ala Gly Glu Ala Val Glu Ala Val Ala Glu Val Phe Arg Glu765576607665Gln Gly Arg Arg Val Ser Gln Leu Arg Val Ser His Ala Phe His767076757680Ser Pro Leu Met Glu Pro Met Leu Ala Asp Phe Arg Glu Val Leu768576907695Ser Gly Val Ser Tyr Ala Glu Pro Ser Leu Pro Val Val Ser Asn770077057710Val Ser Gly Arg Ile Ala Glu Ser Gly Glu Leu Ala Thr Pro Asp771577207725Tyr Trp Val Thr His Val Arg Glu Ala Val Arg Phe Gly Asp Gly773077357740Val Arg Ala Leu Arg Ala Ala Gly Val Asp Arg Cys Val Glu Ile774577507755Gly Pro Asp Ala Val Leu Thr Gly Met Ala Arg Thr Cys Leu Asp776077657770Gly Asp Glu Asp Thr Ala Val Leu Leu Val Pro Ser Ala Arg Lys777577807785Gly Arg Glu Glu Pro Asp Ala Leu Leu Thr Ala Leu Ala Gln Leu779077957800His Thr Ala Gly Thr Ala Val Asp Trp Ala Gly Phe Phe Ala Gly780578107815Ser Gly Ala Gly Pro Val Asp Leu Pro Thr Tyr Ala Phe Gln Arg782078257830Gln Arg Tyr Trp Leu Thr Ala Thr Asp Gly Ala Pro Ala Ala Ala783578407845Gly Ala Gly Leu Asp Ala Ala Asp His Pro Leu Leu Gly Ala Val785078557860Val Ser Leu Pro Asp Ser Gly Gly Ala Val Leu Thr Gly Leu Leu786578707875Ser Val Glu Ala Gln Pro Trp Leu Ala Asp His Val Val Leu Gly788078857890Arg Val Met Leu Pro Gly Ala Val Leu Val Glu Leu Ala Leu Ala789579007905
Ala Gly Glu Ser Val Asp Cys Ala Val Leu Asp Glu Leu Thr Leu791079157920Ala Thr Pro Leu Val Leu Pro Glu His Gly Gly Ala Gln Val Arg792579307935Val Val Val Gly Pro Arg Thr Ala Glu Arg Arg Thr Val Ala Val794079457950Tyr Ser Arg Pro Glu Gly Thr Gly Gln Glu Trp Ala Thr His Ala795579607965Thr Gly Phe Leu Thr Asp Asn Ala Leu Val Ala Gly Ala Glu Ala797079757980Ala Leu Glu Gln Trp Pro Pro Thr Gly Ala Ala Ser Val Pro Val798579907995Asp Ser Ala Tyr Gln Ile Phe Arg Glu Arg Gly Tyr Gly Tyr Gly800080058010Pro Val Phe Gln Gly Leu Arg Ala Ala Trp Arg Arg Gly Gly Glu801580208025Leu Phe Ala Glu Val Ala Leu Pro Glu Glu Ala Ala Ser Glu Ala803080358040Gly Arg Phe Gly Leu His Pro Ala Leu Leu Asp Ala Ala Met His804580508055Val Ala Ile Leu Asn Asp Ser Asn Asp Ser Asn Asp Ser Asn Asp806080658070Asn Ala Glu Gly Gly Ser Gly Gly Thr Val Ile Pro Phe Ala Trp807580808085Asn Arg Val Ala Leu His Ala Val Gly Ala Ala Ala Val Arg Val809080958100Arg Ile Ala Thr Ala Ala Asp Gly Gly Met Asp Ile Arg Val Thr810581108115Asp Val Thr Gly Ala Pro Val Leu Thr Val Gly Ser Met Val Ser812081258130Arg Thr Val Ser Ala Gly Gln Leu Gly Ala Ala Pro Arg Asp Ala813581408145Gly Ala Leu Tyr Ala Pro Glu Trp Val Pro Val Thr Arg Leu Asp815081558160Asp Thr Asp Thr Ala Trp Ala Thr Trp Ser Arg Val Glu Glu Ala816581708175Gly Thr Glu Val Pro Ala Val Val Val Leu Asp Ala Ser Ala Pro818081858190Ala Gly Ala Asp Val Pro Gly Ser Val Arg Ser Ala Leu Asp Gly819582008205Val Leu Thr Val Ala Gln Arg Trp Leu Thr Glu Glu Arg Phe Ala821082158220Ala Ser Arg Leu Val Val Val Thr Arg Gly Ala Met Pro Val Gly822582308235
Ser Gly Gly Ala Pro Ala Glu Gly Asp Val Val Gln Ala Pro Val824082458250Trp Gly Leu Val Arg Ala Ala Leu Ala Glu Asn Pro Gly Arg Phe825582608265Ala Leu Ala Asp Val Ala Val Asp Ala Asp Asp Ala Ala Val Asp827082758280Cys Ala Val Ala Ala Val Leu Ser Gly Glu Ala Glu Val Ala Val828582908295Arg Asp Gly Val Val Leu Val Pro Arg Leu Thr Arg Leu Thr Ala830083058310Asp Ala Pro Ala Ala Glu Leu Pro Leu Ser Val Pro Ser Leu Asp831583208325Gly Glu Gly Ala Val Leu Val Thr Gly Gly Thr Gly Gly Leu Gly833083358340Ala Val Met Ala Arg Tyr Leu Val Ala Glu Arg Gly Val Arg Asp834583508355Leu Val Leu Val Ser Arg Arg Gly Gly Asp Ala Pro Gly Ala Ala836083658370Glu Leu Ala Gly Glu Leu Arg Glu Ala Gly Ala Ala Val Glu Val837583808385Val Ala Cys Asp Leu Ser Asp Arg Glu Ser Val Val Asp Leu Val839083958400Gly Ser Leu Val Ser Gly Arg Gly Leu Leu Ala Val Val His Ala840584108415Ala Gly Val Gly Asp Asn Gly Leu Leu Gly Ser Leu Thr Pro Asp842084258430Arg Phe Asp Ala Val Leu Gly Ala Lys Ala Asp Ala Ala Trp Trp843584408445Leu His Glu Ala Thr Ala Gly Val Glu Leu Ala Ala Phe Val Leu845084558460Val Ser Ser Ala Gly Gly Leu Val Leu Thr Ala Gly Gln Gly Asn846584708475Tyr Ala Ala Ala Asn Val Phe Leu Asp Ala Leu Ala Ser Arg Arg848084858490Arg Ala Glu Gly Leu Val Ala Thr Ser Met Ala Phe Gly Phe Trp849585008505Asp Val Gly Ala Gly Leu Gly Glu Tyr Leu Ser Glu Val Asp Arg851085158520Arg Arg Met Ala Ser Gln Gly Leu Pro Leu Leu Ser His Glu Ala852585308535Gly Leu Glu Leu Phe Ala Ala Gly Leu Asp Arg Ser Glu Ala Thr854085458550Val Val Pro Leu Arg Val Asp Thr Ala Ala Leu Arg Thr Arg Thr855585608565
Asp Glu Ile Pro Ala Leu Leu Arg Ala Leu Ala Pro Ala Arg Arg857085758580Ala Thr Ala Ala Ser Gly Ser Ala Ala Ala Pro Gly Pro Asp Asp858585908595Ser Pro Ser Arg Arg Leu Ala Glu Leu Pro Ala Pro Glu Arg His860086058610Arg Ala Val Leu His Leu Val Arg Ser Gln Val Ala Ala Val Leu861586208625Gly His Gly Ser Ala Glu Ala Ile Gly Ala Asp Arg Ala Phe Gln863086358640Glu Leu Gly Phe Asp Ser Leu Ala Ala Thr Glu Leu Arg Asn Gln864586508655Leu Asn Thr Leu Thr Gly Leu Arg Leu Pro Ala Thr Leu Val Phe866086658670Asp His Pro Asn Ala Leu Ala Val Thr Glu Val Val Glu Glu Glu867586808685Leu Ala Ala Ala His Pro Ala Ser Gly Ala Gly Gly Ala Thr Gly869086958700Asp Asp Asp Gly Val Arg Arg Ala Leu Ser Ala Ile Pro Ala Arg870587108715Arg Leu Arg Asp Ala Gly Leu Ala Glu Thr Leu Leu Glu Leu Ala872087258730Ala Asp Ser Asp Glu Met Ser Asp Thr Asp Arg Asp Ala Leu Leu873587408745Ala Ala Phe Asp Gly Asp Asp Glu Asp Asp Ala Ser Gly Pro Tyr875087558760Asp Glu Ser Asp Gly Ala Ala Glu Thr Ala Gly Thr Gly Thr Ala876587708775Thr Glu Pro Gly Ala Asp Ala Leu Arg Ala Ala Arg Ala Glu Thr878087858790Ala Arg Leu Arg Arg Asp Asn Arg Arg Leu Thr Ser Ala Gln His879588008805Glu Pro Ile Ala Ile Val Gly Met Ala Cys Arg Tyr Pro Gly Gly881088158820Val Ser Ser Pro Glu Asp Leu Trp Arg Leu Val Thr Ser Gly Asp882588308835Asp Ala Ile Val Pro Phe Pro Gln Asp Arg Gly Trp Asp Leu Ser884088458850Ile Leu Arg Asp Pro Asp Gly Asp His Pro Asp Gly Leu Tyr Ala885588608865Arg Glu Gly Gly Phe Leu Asp Gly Ala Ala Asp Phe Asp Pro Ala887088758880Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Gly Met Asp Pro Gln888588908895
Gln Arg Leu Ala Leu Glu Met Ser Trp Glu Ala Leu Glu Arg Ala890089058910Gly Ile Asp Pro Ala Ser Leu Lys Gly Ser Arg Thr Gly Val Phe891589208925Thr Gly Val Met Tyr His Asp Tyr Pro Gly Ser Asp Gly Asn Gly893089358940Ser Val Val Thr Gly Arg Val Ala Tyr Lys Leu Gly Leu Glu Gly894589508955Pro Ala Val Ser Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala896089658970Leu His Leu Ala Val Gln Ala Leu Arg Gln Gly Glu Cys Ser Leu897589808985Ala Val Thr Gly Gly Val Thr Val Leu Ser Thr Pro Ala Val Phe899089959000Ala Glu Phe Gly Arg Gln Gly Gly Leu Ala Pro Asp Gly Arg Cys900590109015Lys Ser Phe Ala Ser Ala Ala Asp Gly Thr Gly Phe Ser Glu Gly902090259030Ala Gly Phe Leu Val Val Glu Arg Leu Ser Asp Ala Val Arg Asn903590409045Gly His Glu Val Leu Ala Val Val Arg Gly Ser Ala Val Asn Gln905090559060Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln906590709075Arg Arg Val Ile Arg Gln Ala Leu Ala Asn Ala Arg Leu Ala Ala908090859090Asp Gln Val Asp Ala Val Glu Ala His Gly Thr Gly Thr Thr Leu909591009105Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln911091159120Asp Arg Pro Glu Asp Arg Pro Leu Leu Leu Gly Ser Val Lys Ser912591309135Asn Ile Gly His Ala Gln Ala Ala Ala Gly Val Ala Gly Val Ile914091459150Lys Met Val Leu Ala Leu Arg Asn Gly Leu Leu Pro Ala Thr Leu915591609165Asn Val Asp Ala Pro Ser Asp Gln Val Asp Trp Thr Thr Gly Ser917091759180Val Glu Leu Leu Thr Glu Ala Arg Gln Trp Thr Gly Ser Gly Arg918591909195Pro Arg Arg Ala Gly Ile Ser Ser Phe Gly Ile Ser Gly Thr Asn920092059210Ala His Val Ile Val Glu Gln Ala Pro Ala Ala Val Pro Ser Asp921592209225
Glu Pro Ala Thr Gly Ala Asp Asp Asp Gly Val Ala Pro Val Val923092359240Pro Trp Leu Leu Ser Ala Arg Asp Arg Asp Ala Leu Arg Ala Gln924592509255Ala Glu Arg Leu Leu His His Leu Thr Gly Asp His Pro Ala Ala926092659270Gly Gly Thr Glu Ala Arg Pro Leu Asp Val Ala Tyr Ser Leu Ala927592809285Thr Ala Arg Thr Ala Phe Glu His Arg Val Ala Val Thr Gly Ala929092959300Gly Arg Asp Gly Leu Leu Ala Ala Leu Gly Ser Val Ala Arg Gly930593109315Glu Glu Pro Ala Thr Thr Thr Gly Glu Gly Leu Leu Ala Leu Leu932093259330Phe Pro Gly Gln Gly Ser Gln Arg Leu Gly Met Gly Arg Asp Leu933593409345Tyr Glu Arg Phe Pro Val Phe Ala Glu Ala Phe Asp Ala Val Cys935093559360Ala Gly Leu Asp Ala His Leu Asp Arg Pro Leu Arg Glu Val Val936593709375Trp Gly Asp Asp Glu Asp Ala Leu Asn Ala Thr Ala Tyr Ala Gln938093859390Ala Ala Leu Phe Ala Leu Gly Val Gly Leu His Arg Leu Val Ala939594009405Ser Trp Gly Val Thr Pro Asp Leu Val Ala Gly His Ser Ile Gly941094159420Glu Ile Ala Ala Ala His Val Ala Gly Val Phe Ser Leu Ala Asp942594309435Ala Cys Ala Leu Val Ala Ala Arg Gly Arg Leu Met Gln Ala Leu944094459450Pro Glu Gly Gly Ala Met Ala Ala Val Gln Ala Thr Glu Asp Glu945594609465Val Leu Ala His Leu Thr Asp Gly Val Ser Val Ala Ala Val Asn947094759480Gly Pro Thr Ser Val Val Val Ser Gly Thr Ala Glu Gly Val Glu948594909495Lys Val Thr Glu Ala Leu Arg Glu Arg Gly Arg Arg Thr Thr Ala950095059510Leu Arg Val Ser His Ala Phe His Ser Pro Leu Met Glu Pro Met951595209525Leu Ala Glu Phe Arg Ala Val Val Ala Gly Leu Ser Pro Gln Ala953095359540Pro Val Ile Pro Val Val Ser Asn Leu Thr Gly Thr Thr Ala Arg954595509555
Ala Glu Glu Leu Cys Ser Ala Glu Tyr Trp Val Arg His Val Arg956095659570Glu Ala Val Arg Phe Ala Asp Gly Val Arg Ala Leu His Glu Ala957595809585Gly Ala Thr Arg Tyr Leu Glu Leu Gly Pro Asp Gly Val Ala Cys959095959600Ala Met Ala Arg Glu Ser Leu Pro Glu Ser Ala Val Thr Val Ala960596109615Ala Gln Arg Arg Thr Val Pro Gly Glu Gln Ala Leu Val Glu Ala962096259630Val Ala Arg Leu His Gly His Gly Val Arg Val Asp Trp Pro Ala963596409645Phe Phe Ala Gly Arg Gly Ala Arg Arg Val Glu Leu Pro Thr Tyr965096559660Ala Phe Gln Arg Arg Arg Phe Trp Pro Ala Gly Met Met Pro Leu966596709675Gly Ala Gly Ala Glu Ala Ala Gly Leu Ala Pro Ala Gly His Pro968096859690Leu Leu Gly Gly Ala Val Glu Leu Ala Gly Thr Asp Gly His Leu969597009705Phe Thr Gly Arg Leu Ser Gln Gly Thr His Gly Trp Ile Ser Asp971097159720His Arg Val Met Gly Ala Val Leu Val Pro Gly Thr Ala Leu Leu972597309735Glu Leu Ala Val His Ala Gly Gly Val Leu Gly Cys Asp Val Val974097459750Glu Glu Leu Thr Leu Ala Ala Pro Leu Val Leu Pro Glu Gln Gly975597609765Thr Val Gln Val Gln Val Gly Val Gly Ala Pro Asp Glu Glu Gly977097759780Arg Arg Gly Ile Thr Ile His Ser Arg Pro Ala Asp Asp Ser Gly978597909795Ala Pro Trp Thr Ser His Ala Glu Gly Thr Leu Ala Ala Gly Pro980098059810Ala Gln Ala Pro Ala Thr Asp Phe Asp Ala Thr Val Trp Pro Pro981598209825Glu Lys Ala Glu Pro Leu Asp Thr Thr Gly Leu Tyr Glu Arg Phe983098359840Glu Asp Ala Gly Phe Ala Tyr Gly Pro Val Phe Gln Gly Leu Arg984598509855Ala Ala Trp Ser Arg Asp Gly Glu Val Phe Ala Glu Ala Ser Leu986098659870Pro Asp Gly Thr Asp Gly Asp Ala Phe Thr Leu His Pro Ala Leu987598809885
Phe Asp Ala Gly Leu His Ala Thr Ala Leu Ala Gly Asp Gly Gly989098959900Glu Ser Glu Gly Thr Gly Gly Val Pro Phe Ser Trp Asn Gly Val990599109915Ser Val His Ala Val Gly Ala Ser Ser Val Arg Leu Arg Leu Ser992099259930Arg Thr Asp Asp Gly Thr Leu Ala Ile Ala Val Thr Asp Thr Ala993599409945Gly Thr Pro Val Ala Ser Val Gly Ser Leu Val Thr Arg Pro Leu995099559960Ser Ala Gly Gln Phe Gly Gly Ala Gly Gly Ala Gly Arg Asp Ser996599709975Leu Phe Gln Leu Asp Trp Ala Ala Ala Gly Asp Pro Ala Thr Arg998099859990Asp Ala Glu Thr Pro Gly Pro Val Ala Val Leu Gly Pro Asp Thr999510000 10005Ala Leu Leu Gly Gly Gly Asp Leu Leu Ala Glu Ala Gly Thr Glu10010 10015 10020Gly Val Thr Gly Leu Ala Asp Leu Val Thr Gly Thr Gly Pro Gly10025 10030 10035Glu Asp Val Pro Ser Val Val Leu Leu Pro Val Ala Thr Ala Pro10040 10045 10050Glu Ala Gly Ser Gly Thr Gly Asp Val Ala Ala Ala Ala His Ala10055 10060 10065Ala Thr Ala Arg Val Leu Glu Thr Leu Arg Asp Trp Ser Ala Gln10070 10075 10080Glu Arg Phe Ala Ala Ser Arg Leu Val Val Val Thr Arg Gly Ala10085 10090 10095Ser Asp Gly Ala Asp Pro Ala Ala Ala Ala Val Trp Gly Leu Val10100 10105 10110Arg Ala Ala Gln Ala Glu Asn Pro Gly Arg Phe Ile Leu Leu Asp10115 10120 10125Leu Glu Pro Ser Ala Thr Ser Pro Leu Gly Met Ser Ala Leu Arg10130 10135 10140Ala Ala Leu Ala Ser Gly Glu Pro Gln Leu Ala Leu Arg Glu Asp10145 10150 10155Arg Leu Leu Ala Pro Arg Leu Val Arg Ala Val Thr Pro Ala Glu10160 10165 10170Ser Asp Ala Ala Gly Trp Asp Pro Glu Gly Thr Val Leu Ile Thr10175 10180 10185Gly Gly Thr Gly Gly Leu Gly Ala Thr Val Ala Arg His Leu Val10190 10195 10200Ala Glu His Gly Val Arg Ser Leu Leu Leu Val Ser Arg Arg Gly10205 10210 10215
Pro Ala Ala Glu Gly Ala Gly Glu Leu Ala Ala Ala Leu Glu Glu10220 10225 10230Ser Gly Ala His Val Thr Val Ala Ala Cys Asp Val Gly Asp Arg10235 10240 10245Thr Ala Val Asp Ala Leu Leu Ala Glu Ile Pro Ala Asp Arg Pro10250 10255 10260Leu Arg Ala Val lle His Ala Ala Gly Val Leu Asp Asp Gly Val10265 10270 10275Leu Asp Ser Leu Thr Pro Glu Arg Leu Ala Ala Val Leu Arg Pro10280 10285 10290Lys Ala Asp Ala Ala Trp His Leu His Glu Ala Thr Glu Gly Leu10295 10300 10305Thr Leu Asp Ala Phe Val Leu Phe Ser Ser Val Ala Gly Thr Leu10310 10315 10320Gly Ser Ala Gly Gln Ala Asn Tyr Ala Ala Gly Asn Ala Phe Leu10325 10330 10335Asp Ala Leu Ala Arg His Arg Arg Asp Arg Gly Leu Pro Ala Val10340 10345 10350Ser Met Ala Trp Gly Pro Trp Thr Arg Gly Ser Gly Met Thr Gly10355 10360 10365Glu Leu Thr Glu Ala Asp Thr Ala Arg Met Ala Arg Ala Gly Met10370 10375 10380Pro Pro Ile Asp Pro Glu Gln Gly Leu Ala Leu Phe Asp Ala Val10385 10390 10395Thr Gly Ala Ala Glu Gln Ala Pro Ala Val Leu Pro Val Arg Leu10400 10405 10410Asp Leu Gly Ala Leu Arg Ser Leu Gly Glu Val Pro Pro Leu Phe10415 10420 10425Arg Ser Leu Ile Arg Thr Gln Ala Arg Arg Ala Ala Ser Asp Gly10430 10435 10440Gly Ala Ala Ala Ala Ala Asp Leu Thr Arg Arg Leu Thr Gly Leu10445 10450 10455Thr Arg Thr Glu Gly Glu Glu Val Leu Leu Asp Leu Val Arg Gly10460 10465 10470Gln Ile Ala Thr Val Leu Gly His Ala Gly Gln Ala Asp Ile Glu10475 10480 10485Pro Ala Arg Ala Phe Gln Asp Leu Gly Phe Asp Ser Leu Thr Ser10490 10495 10500Val Glu Leu Arg Asn Arg Leu Gly Ala Leu Thr Gly Val Arg Leu10505 10510 10515Pro Ala Thr Leu Leu Phe Asp Tyr Pro Thr Pro Gly Glu Leu Val10520 10525 10530Ala His Leu Tyr Thr Arg Val Ala Pro Ala Pro Ala Asp Gly Ser10535 10540 10545
Ala Val Val Leu Ala Glu Leu Asp Lys Leu Glu Arg Ala Phe Ala10550 10555 10560Gly Ala Glu Val Gly Ala Glu Leu Phe Asp Gln Val Ala Gly Arg10565 10570 10575Leu Glu Val Leu Arg Gly Lys Trp Gln Glu Met Arg Ala Glu Ala10580 10585 10590Leu Lys Gly Gly Glu Lys Ala Glu Glu Ser Phe Asp Phe Ala Ser10595 10600 10605Ala Ser Asp Glu Asp Met Phe Asp Met Leu Asp Asn Glu Leu Gly10610 10615 10620Leu Ser10625<210>4<211>5541<212>PRT<213>鏈霉菌FR-008<400>4Met Ser Asn Glu Asp Arg Leu Arg Ala Tyr Leu Lys Arg Ala Val Thr1 5 10 15Asp Leu Gln Glu Ala Arg Gln Gln Leu Ser Asp Ala Glu Asn Arg Asp20 25 30Arg Glu Pro Val Ala Val Val Ala Met Ala Cys Arg Tyr Pro Gly Gly35 40 45Val Arg Thr Pro Gln Asp Leu Trp Glu Leu Ala Arg Asp Gly Val Asp50 55 60Ala Ile Ser Ala Phe Pro Ala Asn Arg Gly Trp Asp Leu Asp His Leu65 70 75 80Phe His Pro Asp Pro Ala His His Gly Thr Thr Tyr Thr Arg Glu Gly85 90 95Gly Phe Leu His Asp Ala Gly Glu Phe Asp Pro Ala Phe Phe Gly Ile100 105 110Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu115 120 125Glu Thr Ser Trp Glu Ala Leu Glu Arg Ala Gly Ile Leu Pro Asp Thr130 135 140Leu Ala Gly Ser Thr Thr Gly Val Phe Val Gly Thr Gly His Gly Asp145 150 155 160Tyr Asp Gly Ala Ser Arg Gly Arg Gln Lys Glu Val Ala Gly His Leu165 170 175Leu Thr Gly Asn Thr Val Ala Val Ala Ser Gly Arg Leu Ser Tyr Thr180 185 190
Tyr Gly Phe Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser195 200 205Ser Leu Val Ala Leu His Leu Ala Val Arg Ser Leu Arg Gln Gly Glu210 215 220Cys Ser Leu Ala Leu Ala Gly Gly Ala Thr Val Met Ser Thr Pro Lys225 230 235 240Met Phe Thr Glu Phe Ser Arg Gln Gln Gly Leu Ala Ala Asp Gly Arg245 250 255Cys Lys Pro Phe Ala Ala Gly Ala Asp Gly Thr Ala Trp Ser Glu Gly260 265 270Val Gly Leu Ile Leu Leu Glu Arg Leu Ser Asp Ala Gln Arg Asn Gly275 280 285His Glu Ile Leu Ala Val Ile Arg Gly Ser Ala Ile Asn Gln Asp Gly290 295 300Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Leu305 310 315 320Ile Arg Gln Ala Leu Ile Asp Ala Gly Leu Thr Ser Gly Glu Val Asp325 330 335Val Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu340 345 350Ala Gln Ala Leu Leu Asn Thr Tyr Gly Gln Asp Arg Gly Asp Ala His355 360 365Pro Leu Leu Leu Gly Ser Leu Lys Ser Asn Thr Gly His Thr Gln Ala370 375 380Ala Ser Gly Val Ala Gly Val Ile Lys Met Val Gln Ala Met Arg His385 390 395 400Gly Thr Leu Pro Lys Ser Leu His Val Asp Ala Pro Thr Pro His Ala405 410 415Asp Trp Ala Thr Gly Gly Val Glu Leu Leu Thr Glu Ser Thr Thr Trp420 425 430Pro Glu Thr Gly Arg Pro Arg Arg Ala Ala Val Ser Ser Phe Gly Val435 440 445Ser Gly Thr Asn Ala His Leu Ile Leu Glu Gln Ala Pro Glu Asp Gln450 455 460Thr Pro Arg Ala Glu Glu Pro Gly Thr Glu Pro Ser Ala Asp Asp Ser465 470 475 480Ala Asp Thr Val Arg Ser Trp Met Val Ser Gly Lys Ser Arg Ala Ala485 490 495Val Gly Glu Gln Ala Ala Arg Leu Ala Ala Ser Val Arg Ala Ser Asp500 505 510Ala Ser Val Leu Asp Val Ala His Ala Leu Val Ser Ser Arg Val Ala515 520 525Met Asp Arg Arg Ala Val Val Val Gly Ser Asp Arg Glu Glu Leu Leu530 535 540
Ala Gly Leu Asp Ala Leu Ala Ala Gly Glu Pro Ser Val Arg Val Val545 550 555 560Glu Gly Thr Ala Gly Glu Gly Ala Gly Asp Val Ala Phe Val Phe Pro565 570 575Gly Gln Gly Ser Gln Trp Val Gly Met Ala Val Glu Leu Leu Gly Ser580 585 590Ser Pro Val Phe Ala Gly Arg Val Ala Glu Cys Ala Ala Ala Leu Glu595 600 605Pro Phe Thr Glu Trp Ser Leu Leu Asp Val Leu Asn Gly Val Glu Gly610 615 620Ala Ala Ser Leu Glu Arg Val Asp Val Val Gln Pro Val Leu Trp Ala625 630 635 640Val Met Val Ser Leu Ala Glu Val Trp Arg Ala Gln Gly Val Glu Pro645 650 655Gly Ala Val Ile Gly His Ser Gln Gly Glu Ile Ala Ala Ala Val Val660 665 670Ala Gly Gly Leu Ser Leu Glu Asp Gly Ala Arg Val Val Ala Leu Arg675 680 685Ser Arg Ala Leu Arg Ala Leu Ser Gly Leu Gly Gly Met Val Ser Val690 695 700Ala Arg Asn Val Asp Glu Val Arg Gly Leu Leu Ala Gly Trp Glu Gly705 710 715 720Arg Ile Ser Val Ala Ala Val Asn Gly Pro Ser Ser Val Val Val Ser725 730 735Gly Asp Ala Glu Ala Leu Asp Glu Phe Val Ala Ala Cys Asp Ala Ser740 745 750Gly Val Arg Ala Arg Arg Val Ala Val Asp Tyr Ala Ser His Ser Ala755 760 765His Val Glu Arg Ile Glu Glu Glu Leu Ala Gly Leu Leu Ala Pro Val770 775 780Ala Pro Arg Ser Cys Asp Val Pro Phe Tyr Ser Thr Leu Ser Gly Gly785 790 795 800Val Ile Asp Thr Ala Gly Leu Asp Ala Gly Tyr Trp Tyr Arg Asn Leu805 810 815Arg Gly Thr Val Glu Phe Glu Ala Gly Thr Arg Ala Leu Leu Ala Asp820 825 830Gly Phe Arg Val Phe Val Glu Val Ser Ala His Pro Val Val Ala Thr835 840 845Gly Val Gln Glu Thr Ile Glu Asp Ala Ser Val Gln Ala Gly Val Val850 855 860Gly Thr Leu Arg Arg Asp Glu Gly Gly Leu Glu Arg Phe Ala Leu Ser865 870 875 880Leu Gly Glu Ala Trp Ala Leu Gly Ala Glu Val Asp Trp Glu Thr His885 890 895
Tyr Ala Gly Thr Arg Pro Arg His Val Asp Leu Pro Thr Tyr Pro Phe900 905 910Arg Arg Asp Trp Tyr Trp Leu Thr Pro Ala Glu Ser Ala Ala Ala Ala915 920 925Ala Thr Gly Asp Pro Ala Asp Gly Asp Phe Trp Ser Ala Val Glu Ser930 935 940Gly Asp Leu Thr Arg Leu Ser Ser Val Leu Glu Val Thr Gly Asp Asp945 950 955 960Val Glu Ala Phe Gly Thr Leu Leu Pro Ala Leu Ala Ser Trp Arg Arg965 970 975Arg Arg Thr Leu Arg Ser Thr Leu Asp Thr Trp Arg His Arg Val Thr980 985 990Trp Thr Ala Leu Thr Gly Ala Asp Asp Ala Val Pro Ala Gly Arg Trp995 10001005Pro Ala Leu Val Pro Ala Gly His Ala Asp Asp Pro Trp Val His101010151020Ala Val Leu Gly Ala Leu Thr Gly Arg Gly Leu Arg Val Glu Arg102510301035His Asp Leu Thr Gly Asp Glu Asp Thr Ala Ala Leu Thr Ala Leu104010451050Leu Thr Gly Thr Gly Asp Thr Glu Pro Asp Gly Ala Glu Gly Ile105510601065Ala Gly Val Leu Ser Leu Leu Ala Leu Asp Glu Arg Pro His Pro107010751080Asp His Pro Ala Val Pro Arg Gly Leu Ala Thr Ala Lys Glu Leu108510901095Leu His Ala Leu Ser Gly Thr Glu Ala Ser Val Trp Ala Leu Thr110011051110Arg Gly Ala Val Ala Val Asp Ser Arg Asp Arg Leu Thr Ser Pro111511201125Val Gln Ala Glu Thr Trp Gly Phe Gly Arg Ala Val Ala Leu Glu113011351140Leu Pro Asp Ala Trp Gly Gly Leu Ala Asp Leu Pro Ala Asp Pro114511501155Asp Ala Arg Ser Leu Ala Arg Leu Ala Thr Leu Leu Gly Gly Pro116011651170Glu Asp Gln Ile Ala Val Arg Ser Ser Gly Ala Tyr Ala Arg Arg117511801185Leu Ala Arg Met Pro Leu Pro Glu Pro Ala Arg Val Thr Asp Val119011951200Asp Ala Glu Leu Ala Val Arg Ala Ala Asp Asp Asp Gly Ile Gly120512101215Gly Trp Gly Asp His Asp Thr Val Leu Ile Thr Gly Gly Thr Gly122012251230
Ala Leu Gly Ala His Val Ala Arg Ser Leu Ala Ala Ser Gly Ala123512401245Arg His Leu Val Leu Thr Ser Arg Arg Gly Pro Gly Ser Pro Gly125012551260Ala Val Gly Leu Val Ala Glu Leu Glu Glu Leu Gly Ala Arg Val126512701275Thr Val Ala Asp Cys Asp Val Ala Asp Arg Asp Gln Leu Ala Arg128012851290Leu Leu Asp Ser Leu Pro Ala Gly Leu Pro Leu Thr Gly Val Val129513001305His Ala Ala Gly Val Leu Asp Asp Gly Val Leu Asp Ala Leu Thr131013151320Leu Asp Arg Phe Glu Ala Val Leu Arg Pro Lys Thr Leu Gly Thr132513301335Ala His Leu His Glu Leu Thr Arg Asp His Asp Val Thr Leu Phe134013451350Val Leu Phe Ser Ser Ile Val Gly Val Leu Gly Asn Ala Gly Gln135513601365Ala Ash Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Ala Val Ala Glu137013751380Gln Arg Arg Ala Glu Gly Leu Pro Val Thr Ser Val Ala Trp Gly138513901395Pro Trp Ala Asp Ala Gly Met Ala Thr Ala Asp Val Leu Ala Asp140014051410Arg Met Ser His Asp Gly Leu Ala Pro Met Asp Pro Val Thr Ala141514201425Val Ala Ala Leu Arg Ala Ala Val Ala Glu Gly Val Pro His Ala143014351440Thr Val Leu Asp Val Asp Trp Arg Ala Tyr Gly Thr Ala Met Thr144514501455Ala Ala Arg Pro Ser Pro Leu Val Gly Asp Leu Pro Glu Val Arg146014651470Arg Ala Leu Glu Ala Ala Ala Ala Thr Ala Pro Asp Thr His Ala147514801485Leu Arg Asp Arg Leu Arg Gly Leu Ala Pro Ala Glu Gln Asp Arg149014951500Leu Leu Thr Asp Leu Val Arg Ala Glu Val Ala Thr Ala Leu Arg150515101515His Ser Ser Pro Glu Ala Val Asp Val Asn Arg Ala Phe Lys Asp152015251530Leu Gly Phe Asp Ser Leu Thr Ala Val Glu Val Arg Asn Arg Ile153515401545Thr Ala Ala Thr Asp Val Lys Leu Pro Thr Thr Leu Leu Phe Asp155015551560
Tyr Pro Asn Thr Thr Ala Val Val Ala His Leu Arg Thr Leu Leu156515701575Leu Gly Asp Glu Arg Pro Val Ala Ala Pro Val Val Val Ser Ala158015851590Gly Val Thr Asp Glu Pro Met Ala Val Val Ala Met Ala Cys Arg159516001605Phe Pro Gly Gly Val Thr Thr Pro Glu Glu Leu Trp His Leu Met161016151620Ala Ala Glu Val Asp Ala Val Ser Thr Pro Pro Glu Asp Arg Gly162516301635Trp Asp Leu Asp Ala Leu Tyr Asp Pro Asp Pro Glu Arg His Gly164016451650Thr Thr Tyr Ser Arg Glu Gly Gly Phe Ile Gln Asp Val Ala Gly165516601665Phe Asp Pro Val Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala167016751680Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Thr168516901695Phe Glu Arg Ala Gly Ile Asp Pro Gly Thr Leu Arg Asn Ser Ala170017051710Thr Gly Val Phe Val Gly Ser Ile Thr Thr Asp Tyr Gln Val Arg171517201725Leu Gly Gly Ala Ala Ala Gln Glu Gln Leu Ala Gly His Leu Met173017351740Thr Gly Asn Ala Ser Ser Ile Ala Ser Gly Arg Leu Ser Tyr Thr174517501755Tyr Gly Phe Glu Gly Pro Ala Val Thr Met Asp Thr Gly Cys Ser176017651770Ser Ser Met Val Ala Leu His Leu Ala Leu Gln Ser Leu Arg Thr177517801785Gly Glu Cys Thr Met Ala Leu Ala Gly Gly Val Thr Val Met Ser179017951800Thr Pro Glu Pro Tyr Val Glu Phe Ser Arg Gln Arg Gly Leu Ala180518101815Ala Asp Gly Arg Cys Lys Ala Phe Ala Glu Gly Ala Asp Gly Met182018251830Gly Phe Ala Glu Gly Val Gly Leu Val Leu Leu Glu Arg Leu Ser183518401845Asp Ala Arg Arg Asn Gly His Pro Val Leu Ala Val Val Arg Gly185018551860Ser Ala Leu Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro186518701875Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Asn188018851890
Ala Gly Leu Ala Pro Gly Glu Val Asp Val Val Glu Ala His Gly189519001905Thr Gly Thr Pro Leu Gly Asp Pro Ile Glu Ala Gln Ala Ile Leu191019151920Ala Thr Tyr Gly Gln Asp Arg Asp Pro Ala Arg Pro Leu Leu Leu192519301935Gly Ser Leu Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly194019451950Val Ala Gly Met Met Lys Met Ile Leu Ala Met Arg Gln Gly Thr195519601965Leu Pro Lys Ser Leu His Ile Thr Arg Pro Thr Ser Val Val Asp197019751980Trp Ser Ser Gly Ala Val Arg Leu Val Thr Glu Ser Thr Pro Trp198519901995Pro Glu Thr Gly Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly200020052010Ile Ser Gly Thr Asn Gly His Leu Ile Leu Glu Glu Ala Pro Ala201520202025Pro Glu Pro Ala Glu Asp Glu Pro Ala Asp Pro Phe Thr Glu Pro203020352040Ser Ala Asp Asp Ser Ala Asp Thr Val Arg Ser Trp Met Val Ser204520502055Gly Lys Ser Arg Ala Ala Val Gly Glu Gln Ala Ala Arg Leu Ala206020652070Ala Ser Val Arg Thr Ser Asp Ala Ser Val Leu Asp Val Ala His207520802085Ala Leu Val Ser Ser Arg Val Ala Met Asp Arg Arg Ala Val Val209020952100Val Gly Arg Asp Gly Glu Glu Leu Leu Ala Gly Leu Asp Ala Leu210521102115Ala Ala Gly Glu Pro Ser Pro Arg Leu Val Glu Gly Ser Val Gly212021252130Asp Gly Ser Gly Lys Val Ala Phe Val Phe Pro Gly Gln Gly Ser213521402145Gln Trp Val Gly Met Ala Val Glu Leu Leu Gly Ser Ser Pro Val215021552160Phe Ala Gly Arg Val Ala Glu Cys Ala Ala Ala Leu Glu Pro Phe216521702175Thr Glu Trp Ser Leu Leu Asp Val Leu Asn Gly Val Glu Gly Ala218021852190Ala Ser Leu Glu Arg Val Asp Val Val Gln Pro Val Leu Trp Ala219522002205Val Met Val Ser Leu Ala Glu Val Trp Arg Ala Gln Gly Val Glu221022152220
Pro Gly Ala Val Ile Gly His Ser Gln Gly Glu Ile Ala Ala Ala222522302235Val Val Ala Gly Gly Leu Ser Leu Glu Asp Gly Ala Arg Val Val224022452250Ala Leu Arg Ser Arg Ala Leu Arg Ala Leu Ser Gly Leu Gly Gly225522602265Met Val Ser Val Ala Arg Asn Val Asp Glu Val Arg Gly Leu Leu227022752280Ala Gly Trp Glu Gly Arg Ile Ser Val Ala Ala Val Asn Gly Pro228522902295Ser Ser Val Val Val Ser Gly Asp Ala Glu Ala Leu Asp Glu Phe230023052310Val Ala Ala Cys Asp Ala Ser Gly Val Arg Ala Arg Arg Val Ala231523202325Val Asp Tyr Ala Ser His Ser Ala His Val Glu Arg Ile Glu Glu233023352340Glu Leu Ala Gly Leu Leu Ala Pro Val Ala Pro Arg Ser Cys Asp234523502355Val Pro Phe Tyr Ser Thr Leu Ser Gly Gly Val Ile Asp Thr Ala236023652370Gly Leu Asp Ala Gly Tyr Trp Tyr Arg Asn Leu Arg Gly Thr Val237523802385Glu Phe Glu Ala Gly Thr Arg Ala Leu Leu Ala Asp Gly Phe Arg239023952400Val Phe Val Glu Val Ser Ala His Pro Val Val Ala Thr Gly Val240524102415Gln Glu Thr Ile Glu Asp Ala Ser Val Gln Ala Gly Val Val Gly242024252430Thr Leu Arg Arg Asp Glu Gly Gly Leu Glu Arg Phe Ala Leu Ser243524402445Leu Gly Glu Ala Trp Ala Leu Gly Ala Glu Val Asp Trp Glu Thr245024552460His Tyr Ala Gly Thr Arg Pro Arg His Val Asp Leu Pro Thr Tyr246524702475Ala Phe Gln Arg Gln His Tyr Trp Pro Arg Phe Ala Glu Leu Gly248024852490Gly Asp Val Ser Ser Ala Gly Leu Asp Ser Pro Asp His Pro Leu249525002505Leu Gly Ala Ser Val Glu Leu Ala Gly Gly Asp Gly Leu Val Ala251025152520Thr Ala Arg Trp Ser Leu Arg Thr His Pro Trp Leu Ala Asp His252525302535Ala Val Ser Gly Thr Val Ile Val Pro Gly Thr Ala Leu Val Glu254025452550
Ser Val Ile Arg Ala Gly Asp Val Leu Gly Cys Gly Arg Ile Glu255525602565Glu Leu Ala Leu Gln Ala Pro Val Val Leu Gln Glu Arg Gly Glu257025752580Ala Gln Val Gln Ile Gly Val Gly Glu Pro Asp Ala Ser Gly Arg258525902595Arg Pro Val Thr Val His Thr Arg Thr Thr Gly Pro Asp Gly Ala260026052610Thr Glu Asp Leu Trp Thr Leu Arg Ala Gln Gly Thr Leu Thr Glu261526202625Pro Gly Ala Pro Ala Val Asp Arg Pro Glu Glu Phe Thr Ser Trp263026352640Pro Pro Pro Gly Ala Thr Ala Val Ala Ala Glu Gly Phe Tyr Glu264526502655Leu Leu Ala Gly Arg Gly Tyr Glu Phe Gly Pro Val Phe Gln Gly266026652670Val Arg Ala Thr Trp Ser Arg Gly Glu Asp Leu Tyr Ala Glu Val267526802685Val Leu Pro Glu Gln Val Arg Gly Asp Ala Gly Arg Phe Gly Ile269026952700His Pro Ala Leu Leu Asp Ala Ala Leu His Ala Ala His Leu Ala270527102715Pro Gly Ser Ala Gly Arg Thr Val Val Pro Phe Thr Trp Ser Gly272027252730Val Cys Leu His Ala Ala Gly Ala Thr Ala Leu Arg Val Arg Val273527402745Ser Pro Ala Gly Gln Asp Ala Ile Ser Val Gln Leu Thr Asp Pro275027552760Thr Gly Ala Pro Val Ala Thr Ile Asp Ala Leu Ala Val Arg Glu276527702775Ile Ala Pro Asp Thr Leu Asp Pro Ser Ala Arg Ala Ala Arg Asp278027852790Trp Leu Phe Arg Leu Asp Trp Thr Pro Val Thr Pro Pro Ala Ala279528002805Val Pro Ala Ala Thr Gly Trp Ala Val Leu Gly Thr Pro Ala Asp281028152820Pro Val Thr Ala Pro Asp Gly Ser Gly Thr Thr Leu Pro Ala Phe282528302835Ala Thr Pro Gly Ala Leu Ala Glu His Thr Glu Ala Asn Gly His284028452850Arg Pro Asp Ala Val Leu Leu Phe Ala Gly Ala Ala Ala Ser Gly285528602865Thr Ala Ala Ala Pro Asp Ala Glu Pro Gly Val Pro Asp Ala Val287028752880
Leu Asp Asp Val Leu Ala Thr Val Gln Ser Trp Leu Ala Asp Glu288528902895Thr Thr Glu Gly Val Pro Leu Val Val Val Thr Arg Gly Ala Val290029052910Pro Thr Gly Pro Ala Asp Pro Ala His Asp Leu Pro Gly Ala Ala291529202925Val Trp Gly Leu Val Arg Ser Ala Gln Ser Gln His Pro Gly Arg293029352940Leu Val Leu Val Asp Leu Asp Ile His Asp Asp Ser Trp Leu Ala294529502955Leu Pro Ala Ala Leu Ala Ala Gly Glu Pro Gln Leu Ala Leu Arg296029652970Gln Gly Ala Ala Tyr Ala Pro Arg Leu Val Arg Pro Arg Thr Ser297529802985Asp Ala Leu Thr Val Pro Glu Gly Thr Gly Asn Trp Arg Leu Asp299029953000Ile Pro Glu Lys Gly Ser Val Asp Arg Leu Asp Leu Val Ala Ala300530103015Pro Gly Thr Ala Glu Glu Pro Ala Ala Gly Glu Val Val Ile Glu302030253030Val Arg Ala Ala Gly Leu Asn Phe Arg Asp Val Leu Asn Thr Leu303530403045Gly Met Tyr Pro Gly Pro Ala Val Leu Leu Gly Ala Glu Ala Ala305030553060Gly Val Val Thr Ala Val Gly Glu Gly Val Thr Gly Phe Ala Pro306530703075Gly Asp Arg Val Met Gly Leu Val Ser Gly Gly Phe Ala Pro Arg308030853090Ala Val Ala Asp Ala Arg Met Leu Ala Pro Val Pro Asp Gly Trp309531003105Thr Trp Ala Gln Ala Ala Ser Val Pro Val Ala Phe Leu Thr Ala311031153120Tyr Tyr Gly Leu Arg Asp Leu Ala His Leu Asp Ala Gly Glu Ser312531303135Val Leu Val His Ala Ala Ala Gly Gly Val Gly Met Ala Ala Ala314031453150Gln Leu Ala Arg His Trp Gly Ala Glu Val Tyr Gly Thr Ala Gly315531603165Asp His Lys Arg Thr Leu Leu Arg Ala Asp Gly Trp Asp Asp Ala317031753180His Leu Ala Ser Ser Arg Thr Leu Ala Phe Glu Asp Arg Phe Arg318531903195Thr Thr Ser Gly Gly Arg Gly Val Asp Val Val Leu Asn Ser Leu320032053210
Ala Gly Asp Tyr Ile Asp Ala Ser Leu Arg Leu Leu Ala Pro Gly321532203225Gly Arg Leu Val Asp Met Gly Lys Thr Asp Val Arg Asp Pro Gly323032353240Gln Val Leu Arg Asp His Pro Ala Ala Ala Leu Tyr Gln Ala Tyr324532503255Glu Leu Arg Glu Ala Gly Glu Asp Arg lle Gln Glu Met Phe Arg326032653270Asp Leu Ser Glu Leu Phe Ala Ser Gly Ala Leu Arg Pro Leu Pro327532803285Thr Val Thr Trp Asp Val Arg His Ala Arg Glu Ala Phe Arg His329032953300Met Ala Gln Ala Lys His Tyr Gly Lys Ile Val Leu Thr Leu Pro330533103315Arg Ala Trp Asp Pro Glu Gly Thr Val Leu Val Thr Gly Gly Ala332033253330Gly Val Leu Gly Gly Ile Leu Ala Arg His Leu Val Thr Arg His333533403345Gly Met Arg His Leu Val Leu Thr Gly Arg Arg Gly Pro Asp Thr335033553360Pro Gly Ala Gly Glu Leu Ala Ala Glu Leu Arg Glu Leu Gly Ala336533703375Glu Val Thr Leu Ala Ala Cys Asp Ala Ala Asp Ala Asp Ala Leu338033853390Ala Ala Val Leu Ala Ala Val Pro Ala Glu His Pro Leu Thr Ala339534003405Val Val His Ala Ala Gly Val Leu Asp Asp Gly Val Leu Glu Ser341034153420Leu Thr Pro Asp Arg Leu Arg Thr Val Leu Ala Pro Lys Ala Asp342534303435Ala Ala Arg His Leu His Arg Leu Thr Arg Asp Leu Asp Leu Thr344034453450Asp Leu Val Leu Phe Ser Ala Gly Ala Gly Val Phe Gly Asn Ala345534603465Gly Gln Ala Asn Tyr Ala Ala Ala Asn Thr Tyr Leu Asp Ala Leu347034753480Ala His Arg Arg Arg Ala Asp Gly Leu Pro Thr Thr Ser Leu Ser348534903495Trp Gly Leu Trp Ala Asp Thr Ser Ala Leu Thr Gly Gln Leu Asp350035053510Asp Thr Asp Leu Ala Arg Val Arg Arg Ser Gly Val Leu Pro Leu351535203525Ser Ala Ala Asp Gly Met Val Leu Phe Asp Ala Ala Leu Thr Glu353035353540
Gln Arg Pro His Leu Val Pro Val Arg Leu Glu Thr Gly Gln Leu354535503555Arg Ala Ser Gly Asp Glu Val Pro Pro Leu Leu Arg Ser Leu Tyr356035653570Arg Gly Pro Val Arg Arg Thr Ala Glu Ala Gly Ala Ala Thr Gly357535803585Thr Asp Gly Leu Arg Ala Arg Leu Ala Gly Arg Ser Ala Glu Asp359035953600Arg Leu Ser Thr Leu Arg Asp Leu Val Gly Ala His Ala Ala Ala360536103615Val Leu Gly His Asp Asp Thr Glu Pro Phe His Ala Gly Arg Ala362036253630Phe Arg Asp Leu Gly Phe Asp Ser Leu Thr Ala Val Glu Leu Arg363536403645Asn Arg Leu Ser Ala Ala Thr Gly Leu Arg Leu Pro Ala Thr Leu365036553660Val Phe Asp His Pro Thr Val Ala Ala Leu Ala Ala His Leu Asp366536703675Gly Glu Leu Ser Asp Gly Thr Pro Ala Ala Leu Pro Ala Ala Pro368036853690Ala Pro Ala Pro Ala Ala Pro Gly Asp Asp Asp Pro Phe Ala Ile369537003705Val Gly Met Ala Cys Arg Leu Pro Gly Asp Ile His Ser Pro Glu371037153720Asp Leu Trp Arg Leu Leu Ala Asp Gly Ala Asp Ala Val Ala Pro372537303735Phe Pro Asp Asp Arg Gly Trp Asp Leu Ala Arg Leu Tyr Asp Ser374037453750Val Pro Glu Gly Gln Gly Ser Ser Arg Thr Arg Glu Gly Gly Phe375537603765Leu His Asp Ala Ala Asp Phe Asp Ala Asp Phe Phe Gly Ile Ser377037753780Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu378537903795Glu Thr Ala Trp Glu Thr Phe Glu Arg Ala Gly Ile Asp Pro Arg380038053810Ser Val Arg Gly Ser Arg Thr Gly Val Phe Ala Gly Leu Ser Ser381538203825Ser Asp Tyr Leu Lys Arg Val Thr His Val Pro Asp Glu Ile Ala383038353840Gly Tyr Val Asn Asn Gly Asn Ala Asn Ser Ile Val Ser Gly Arg384538503855Val Ser Tyr Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Val Asp386038653870
Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Met Ala Leu Gln387538803885Ala Leu Arg Arg Gly Glu Cys Thr Met Ala Leu Ala Gly Gly Val389038953900Thr Val Met Ser Ser Pro Glu Ile Ile Val Asp Phe Ser Arg Gln390539103915Arg Gly Leu Ala Ala Asp Gly Arg Cys Lys Pro Phe Ala Glu Ala392039253930Ala Asp Gly Thr Gly Phe Ser Glu Gly Val Gly Leu Ile Leu Val393539403945Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Gln Val Leu Ala395039553960Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly396539703975Leu Ser Ala Pro Asn Gly Pro Ala Gln Gln Arg Val Ile Arg Gln398039853990Ala Leu Ala Asp Ala Gly Val Gly Ala Ala Glu Val Asp Val Val399540004005Glu Ala His Gly Thr Gly Thr Arg Leu Gly Asp Pro Ile Glu Ala401040154020Gln Ala Leu Leu Ala Thr Tyr Gly Arg Asp Arg Asp Gly Gly His402540304035Pro Leu Phe Leu Gly Ser Val Lys Ser Asn Leu Gly His Thr Gln404040454050Ala Ala Ala Gly Val Ala Gly Val Met Lys Met Val Leu Ala Leu405540604065Arg His Arg Leu Leu Pro Glu Ser Leu His Ile Asp Ala Pro Thr407040754080Pro His Val Asp Trp Ala Asp Ser Gly Val Arg Leu Leu Thr Glu408540904095Ala Thr Ala Trp Pro Glu Ala Gly Ala Ser Arg Pro Arg Arg Ala410041054110Ala Val Ser Ser Phe Gly Ile Ser Gly His Gln Arg Pro Arg His411541204125Pro Gly Gly Gly Pro Pro Pro Pro Pro Ala Arg Thr Ala Ala Ser413041354140Arg Ser Pro Pro Ala Pro Asp Ala Ala Glu Thr Arg Pro Ala Pro414541504155Leu Pro Trp Val Leu Ser Ala Arg Ser Glu Ala Ala Leu Arg Gly416041654170Gln Ala Lys Ala Leu Leu Thr His Leu Asp Ala His Pro Gly Thr417541804185Asp Pro Ala Asp Ile Ala His Thr Leu Val Thr Arg Arg Ala Gln419041954200
Leu Glu Lys Arg Ala Val Val Val Gly Ser Ala Thr Gly Asp Phe420542104215Arg Ala Gly Leu Ala Ala Leu Ala Glu Gly Ser Pro Ala Ala His422042254230Val Val Thr Gly Gly Arg Gly Ala Gly Arg Asp Arg Arg Thr Val423542404245Leu Val Phe Pro Gly Gln Gly Ser Gln Trp Ala Gly Met Gly Ala425042554260Glu Leu Leu Asp Ala Ser Pro Ala Phe Ala Asp Arg Val Ala Ala426542704275Cys Glu Glu Ala Leu Ala Pro Tyr Val Asp Trp Ser Leu Thr Ala428042854290Val Leu Arg Gln Glu Glu Gly Ala Pro Ala Leu Asp Arg Val Asp429543004305Val Val Gln Pro Val Thr Trp Ala Val Met Val Ala Leu Ala Glu431043154320Val Trp Arg Ala His Gly Leu Arg Pro Ala Ala Val Leu Gly His432543304335Ser Gln Gly Glu Ile Ala Ala Ala Ala Val Ala Gly Ala Leu Ser434043454350Leu Ala Asp Ala Ala Arg Ile Val Ala Val Arg Ser Arg Ala Ile435543604365Ala Arg Glu Leu Ser Gly His Gly Gly Met Val Ala Val Thr Ala437043754380Pro His Asp Glu Val Thr Ala Leu Leu Ala Asp Leu Pro Gly Val438543904395Cys Val Ala Ala Val Asn Gly Pro Ser Ser Val Val Val Ser Gly440044054410Asp Thr Gly Gly Leu Asp Thr Leu Leu Ala Ala Cys Glu Glu Gln441544204425Gly Val Arg Ala Arg Arg Val Pro Val Asp Tyr Ala Ser His Ser443044354440Ala His Val Asp Arg Leu Ala Glu Ser Leu Pro Ala Ala Leu Asp444544504455Gly Ile Glu Pro Arg Asp Gly Asp Ile Pro Phe Phe Ser Thr Val446044654470Thr Ala Asp Trp His Pro Gly Thr Gly Leu Gly Ala Ala Tyr Trp447544804485His Arg Asn Leu Arg Gly Thr Val Arg Leu Glu Glu Ser Leu Arg449044954500Ala Leu Val Glu Gln Gly His Asp Val Phe Val Glu Cys Gly Pro450545104515His Pro Val Leu Thr Val Gly Ile Glu Asp Thr Val Ala Ala Leu452045254530
Gly Ala Asp Ala Val Ala Leu Gly Ser Leu Arg Arg Asp Asp Gly453545404545Gly Pro Ala Arg Val Leu Thr Ala Leu Ala Ala Ala His Val Glu455045554560Gly Val Pro Val Asp Trp Arg Pro Ala Val Ser His Gly Thr Ala456545704575Val Asp Leu Pro Thr Tyr Ala Phe Gln Arg Arg Arg Tyr Trp Leu458045854590Asp Ala Gly Thr Pro Gln Gly Asp Pro Ala Gly Leu Asp Ser Val459546004605Val Arg Leu Ala Asp Gly Gly Ala Val Leu Ser Gly Ser Leu Ser461046154620Leu Thr Thr Gln Pro Trp Leu Asp Ala His Arg Thr His Gly Ala462546304635Ala Val Val Pro Thr Thr Ala Leu Leu Asp Trp Ala Val Arg Ala464046454650Gly Asp Glu Thr Gly Leu Pro Val Val Ala Ala Leu Asp Glu His465546604665Ser Pro Leu Val Val Pro Glu Glu Gly His Val Glu Ile Gln Leu467046754680Thr Val Ser Ala Ala Asp Thr Asp Ser Arg Pro Phe Ser Val His468546904695Ser Arg Leu His Ala Ala Asp Ala Gly Glu Pro Ala Thr Pro Trp470047054710Thr Arg Asn Ala Thr Gly Asp Leu Thr Asp Arg Pro Thr Thr Thr471547204725Pro Gln Ala Ser Gly Thr Trp Pro Pro Ala Asp Ala Val Ala Ala473047354740Asp Pro Ala Pro Val Arg Ala Leu Ala Glu Asp Arg Gly Leu Asp474547504755Leu Ala Ala Pro Phe Asp Thr Val Arg Ser Leu Trp Arg Ala Gly476047654770Thr Asp Val Leu Ala Glu Val Ala Leu Pro Asp Ala Ala His Ala477547804785Asp Ala Ala Arg Phe Arg Leu His Pro Ala Leu Leu Gln Thr Pro479047954800Leu Ala Leu Ala Ala Thr Thr Glu Glu Ala Thr Ala Pro Val Leu480548104815Pro Ala Ala Trp Arg Asp Val Thr Val His Ala Thr Gly Ala Ser482048254830Arg Leu Arg Val Arg Leu Thr Pro Gly Asp Asp Ala Thr Trp Ser483548404845Val Glu Ala Arg Asp Ala Ala Gly Asp Leu Val Leu Thr Gly Thr485048554860
Ala Val Thr Arg Ser Ala Gly Pro Asp Arg Leu Pro Ala Thr Ala486548704875Gly Pro Asp Ala Pro His Arg Val Ala Trp Leu Pro Trp Thr Asp488048854890Thr Thr Ser Ser Ala Thr Pro Glu Pro His Gly Pro Trp Ala Val489549004905Ile Gly Ala Pro Gly Arg Leu Thr Ala Ala Leu Glu Arg Ser Gly491049154920Ala Thr Val Gln Leu His Pro Asp Leu Ala Ala Leu Thr Thr Ala492549304935Leu Asp Asn Asp Glu Ala Ala Pro Pro Ala Leu Val Val Ala Phe494049454950His Gly His Asp Ser Gly Glu Asp Pro Thr Lys Ala Ala His Thr495549604965Ser Ala Ala Arg Ala Leu Thr Leu Ser Thr Ala Trp Leu Arg Glu497049754980Pro Arg Leu Ala Asp Ala Arg Leu Leu Leu Val Thr Glu Gly Ala498549904995Val Ala Thr Gly Pro Asp Asp Thr Val Pro Gly Leu Ala Asp Ala500050055010Thr Ala Trp Gly Leu Leu Arg Ser Ala Gln Thr Glu His Pro Gly501550205025Arg Phe Leu Leu Ala Asp Leu Asp Pro Ala Pro Gly Asp Thr Asp503050355040Glu Asp Ser Ala Ala Asp Ala Leu Ile Ala Ala Ala Ala Ala Ala504550505055Ala Arg Thr Gly Glu His Gln Phe Ala Val Arg Ala Gly Thr Val506050655070Thr Val Pro Arg Leu Val Pro Ala Gly His Pro Glu Gly Ala Pro507550805085Ala Pro Ala Ala Thr Ala Pro Trp Gly Glu Gly Thr Gly Ala Gly509050955100Thr Val Leu Ile Thr Gly Gly Thr Gly Thr Leu Gly Ala Leu Val510551105115Ala Arg His Leu Val Thr Arg His Gly Val Arg His Leu Leu Leu512051255130Thr Ser Arg Arg Gly Pro Gly Ala Pro Gly Ala Ala Ala Leu Arg513551405145Glu Glu Leu Ala Ala Leu Gly Ala Glu Ala Glu Ile Val Ala Cys515051555160Asp Val Ala Asp Arg Glu Gln Leu Ala Gly Val Leu Asp Ala Val516551705175Pro Ala Glu His Pro Leu Thr Ala Val Val His Thr Ala Gly Val518051855190
Leu Asp Asp Gly Thr Leu Asp Gly Leu Thr Ala Asp Arg Val Ala519552005205His Val Leu Arg Pro Lys Ala Asp Ala Ala Trp His Leu His Glu521052155220Leu Thr Arg Asp Thr Lys Leu Thr Ala Phe Val Met Phe Ser Ser522552305235Tyr Ala Gly Val Ala Gly Gly Pro Gly Gln Ala Asn Tyr Ala Ala524052455250Ala Asn Ala Phe Leu Asp Ala Leu Ala Gln His Arg Arg Ala Ser525552605265Gly Leu Pro Ala His Ser Leu Ala Trp Gly Leu Trp Glu Asp Arg527052755280Ser Asp Leu Thr Gly Ala Leu Asp Thr Thr Gly Leu Ala Arg Leu528552905295Glu Arg Ser Gly Ile Arg Pro Leu Thr Ala Gly Gln Gly Leu Ala530053055310Leu Leu Asp Ala Ser Thr Ala Leu Asp Thr Ala His Leu Val Pro531553205325Val Arg Leu Asp Thr Arg Thr Leu Arg Ala Asp Glu Val Pro Pro533053355340Leu Leu Arg Ala Leu Ala Arg Pro Ala Val Arg Arg Ala Ala Asp534553505355Ser Gly Pro Gly Ala Ala Thr Gly Ala Pro Ala Asp Leu Arg Glu536053655370Arg Leu Ala Gly Leu Ser Gly Pro Gln Gln Arg Ala Leu Leu His537553805385Arg Thr Val Leu Gly His Leu Ala Ala Val Leu Gly His Ala Ser539053955400Ala Ala Ser Leu Asp Ala Asp Arg Gly Phe Leu Asp Leu Gly Met540554105415Ser Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Asn Ala Asp542054255430Thr Gly Leu Ser Leu Pro Thr Thr Leu Ile Phe Asp His Pro Asp543554405445Pro Ala Ala Leu Val Arg His Leu Gln Thr Glu Leu Gly Thr Gly545054555460Ala Gly Glu Pro Asp Gln Ala Val Phe Ala Glu Leu Ala Ala Leu546554705475Glu Ala Ala Val Gly Gly Ala Ala Leu Asp Asp Gln Asp Arg Ala548054855490Arg Leu Ala Gln Arg Leu Lys Ala Leu Gly Trp Lys Leu Asp Ala549555005505Thr Ser Gln Asp Ala Asp Glu Pro Asp Asp Asp Ser Asp Leu Asp551055155520
Thr Thr Thr Asp Asp Glu Met Phe Asp Leu Ile Asp Asn Glu Leu552555305535Gly Leu Ala5540<210>5<211>2049<212>PRT<213>鏈霉菌FR-008<400>5Met Pro Asp Asp Lys Lys Leu Val Asp Tyr Leu Lys Trp Val Thr Ala1 5 10 15Asp Leu His Lys Thr Arg Arg Arg Leu Glu Glu Ala Glu Ala His Arg20 25 30Arg Glu Pro Ile Ala Val Val Gly Met Ala Cys Arg Leu Pro Gly Gly35 40 45Val Ala Thr Pro Glu Glu Tyr Trp Arg Leu Leu Glu Glu Gly Arg Asp50 55 60Gly Ile Ala Pro Phe Pro Ala Asp Arg Gly Trp Asp Leu Asp Ala Leu65 70 75 80Asn Gly Glu Gly Ala Gly Ser Ser Ala Ala Gly Glu Gly Gly Phe Val85 90 95Asp Ala Ala Ala Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu100 105 110Ala Val Ala Met Asp Pro Gln Gln Arg Ile Leu Leu Glu Thr Thr Trp115 120 125Glu Ala Val Glu Arg Ala Gly Ile Asp Pro Val Ser Leu Arg Gly Ser130 135 140Arg Thr Gly Val Tyr Val Gly Thr Ala Gly Val Asp Tyr Val Gly Val145 150 155 160Val Met Asn Ser Arg Glu Asp Ala Glu Gly His Ala Thr Thr Gly Leu165 170 175Thr Ala Ser Val Val Ser Gly Arg Leu Ser Tyr Ala Phe Asp Leu Ala180 185 190Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ala Ser Leu Val Ala195 200 205Ile His Leu Ala Ala Gln Ala Leu Arg Asn Gly Glu Cys Gly Leu Ala210 215 220Leu Ala Gly Gly Val Thr Val Met Ser Thr Pro Met Gly Phe Ser Gly225 230 235 240Phe Thr Arg Gln Gly Gly Ile Ser Thr Ser Gly Arg Cys Lys Ala Phe245 250 255
Ala Asp Ser Ala Asp Gly Thr Gly Trp Ala Glu Gly Ala Gly Val Leu260 265 270Val Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Gln Val Leu275 280 285Ala Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly290 295 300Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln Ala305 310 315 320Leu Ala Gly Ala Gly Leu Ser Ala Ala Asp Val Asp Ala Val Glu Ala325 330 335His Gly Thr Gly Thr Pro Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu340 345 350Leu Ala Thr Tyr Gly Gln Asp Arg Pro Glu Asp Arg Pro Leu Leu Leu355 360 365Gly Ser Val Lys Ser Asn Ile Gly His Ser Gln Ala Ala Ala Gly Val370 375 380Ala Gly Val Ile Lys Thr Val Leu Ala Ile Gln His Gly Val Leu Pro385 390 395 400Arg Ser Leu His Ile Asp Glu Ala Ser Ser His Val Asp Trp Gly Ala405 410 415Gly Asn Val Arg Leu Leu Thr Glu Asn Thr Ala Trp Pro Glu Thr Gly420 425 430Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Ile Ser Gly Thr Asn435 440 445Ala His Val Val Ile Glu Gln Ala Pro Glu Pro Glu Glu Pro Ala Thr450 455 460Pro Glu Asp Gly Thr Pro Thr Ala Arg Pro Thr Leu Val Pro Trp Pro465 470 475 480Val Ser Ala Lys Thr Arg Glu Ala Leu Asp Ala Gln Leu Ala Arg Val485 490 495Thr Ser Val Thr Gly Ala Ala Pro Leu Asp Ile Gly His Ser Leu Ala500 505 510Thr Gly Arg Ser Thr Phe Glu His Arg Ala Val Leu Leu Ala Gly Pro515 520 525Glu Gly Glu Pro Ala Glu Ala Ala Arg Gly Arg Ala Thr Glu Arg Thr530 535 540Leu Ala Leu Leu Phe Ser Gly Gln Gly Ser Gln Arg Ala Gly Met Gly545 550 555 560Arg Glu Leu Tyr Glu Arg Phe Pro Val Phe Ala Glu Ala Leu Asp Ala565 570 575Val Val Ala Arg Leu Asp Thr Gly Leu Glu Arg Ser Leu Arg Glu Val580 585 590Leu Phe Val Glu Glu Gly Ser Glu Ala Ala Ala Leu Leu Asp Val Thr595 600 605
Gly Tyr Thr Gln Pro Ala Leu Phe Ala Val Glu Val Ala Leu Phe Arg610 615 620Leu Val Glu Ser Trp Gly Val Ser Pro Glu Phe Val Ala Gly His Ser625 630 635 640Val Gly Glu Ile Ala Ala Ala His Val Ala Gly Val Phe Ser Leu Asp645 650 655Asp Ala Cys Arg Leu Val Ala Ala Arg Ala Arg Leu Met Gln Glu Leu660 665 670Pro Thr Gly Gly Ala Met Val Ala Val Arg Ala Thr Glu Ala Glu Val675 680 685Thr Pro Arg Leu Thr Glu Gly Leu Ser Leu Ala Ala Val Asn Gly Pro690 695 700Asp Ser Val Val Val Ser Gly Pro Glu Gly Glu Val Thr Ala Leu Ala705 710 715 720Ala Glu Phe Glu Thr Glu Gly Arg Lys Ile Gln Arg Leu Ser Val Ser725 730 735His Ala Phe His Ser Ala Leu Met Glu Pro Met Leu Asp Ala Phe Arg740 745 750Glu Val Ala Arg Ser Leu Glu Tyr Ala Glu Pro Arg Val Pro Val Val755 760 765Ser Asn Val Thr Gly Ala Leu Ala Ala Pro Gly Gln Leu Thr Asp Pro770 775 780Glu Tyr Trp Val Arg His Val Arg Glu Thr Val Arg Phe Ala Asp Gly785 790 795 800Val Arg Thr Leu Ala Glu Ala Gly Ala Asp Ala Phe Leu Glu Ile Gly805 810 815Pro Gly Gly Val Leu Thr Ala Leu Ala Arg Gln Ser Leu Asp Thr Leu820 825 830Asp Gly Gly Thr Gly Ala Val Ala Ala Pro Ala Leu Arg Ser Arg Arg835 840 845Pro Glu Gln Glu Ala Leu Leu Thr Gly Leu Ala Gln Leu His Val Ala850 855 860Gly Val Arg Val Asp Trp Ala Ala Trp Phe Thr Gly Thr Gly Ala Arg865 870 875 880Arg Thr Asp Leu Pro Thr Tyr Ala Trp Gln His Arg Arg Tyr Trp Pro885 890 895Arg Pro Leu Ala His Ala Ala Asp Ile Pro Gly Ala Gly Leu Thr Pro900 905 910Ala Glu His Pro Leu Leu Gly Ala Ala Val Ser Leu Ala Gly Ser Glu915 920 925Gly Val Leu Leu Thr Gly Arg Val Gly Leu Arg Thr His Pro Trp Leu930 935 940Ala Asp His Val Met Gly Gly Leu Val Leu Phe Pro Ala Thr Ala Phe945 950 955 960
Leu Glu Leu Ala Val Arg Ala Gly Asp Gln Ala Gly Cys Gly Arg Val965 970 975Gln Glu Leu Thr Leu Thr Thr Pro Leu Ala Leu Asp Pro Arg Gly Ala980 985 990Ala Ser Val Gln Val Gly Val Gly Pro Pro Asp Ala Ala Gly Cys Arg995 10001005Pro Leu Ala Ile His Ser Arg Pro Glu Asp Ala Ala Asp Asp Glu101010151020Pro Trp Thr Gln His Ala Thr Gly Val Leu Val Ala Gly Ala Arg102510301035Gln Val Ala Phe Gly Gln Ser Ala Trp Pro Pro Ala Asp Ala Thr104010451050Pro Val Asp Leu Asp Gly Phe Tyr Glu Arg Thr Glu Tyr Gly Pro105510601065Leu Phe Gln Gly Leu Arg Ala Val Trp Thr Arg Gly Asp Glu Val107010751080Leu Ala Glu Leu Glu Leu Pro Ala Arg Ala Asp Asp Ala Ala Leu108510901095Phe Gly Leu His Pro Ala Leu Leu Thr Ala Ala Leu His Ala Val110011051110Glu Tyr Val Glu Leu Lys Asp Ala Asp Gln Gly Leu Leu Pro Phe111511201125Ser Trp Ser Gly Val Thr Leu His Ala Ser Gly Ala Ala Arg Leu113011351140Arg Val Arg Ile Thr Lys Ala Gly Glu Asp Thr Val Ser Ile Ala114511501155Ala Val Asp Thr Ala Gly Arg Pro Val Leu Ser Val Glu Ser Leu116011651170Ala Leu Arg Pro Ser Gly Ala Ala Arg Leu Leu Lys Pro Gly Thr117511801185Asp Arg Ser Thr Leu Leu Gly Leu Asp Trp Val Pro Ala Glu Pro119011951200Thr Gly Pro Ala Ala Gly Gly Pro Arg Val Ala Leu Gly Thr Asp120512101215Pro Phe Gly Thr Gly Thr Val Leu Ala Gly Leu Asp Glu Val Thr122012251230Glu Thr Gly Thr Gln Val Leu Val Pro Val Pro Asp Gly Gly Glu123512401245Asp Pro Val Thr Ala Thr His Glu Ala Thr Arg Ala Thr Leu Ala125012551260Leu Leu Gln Glu Trp Leu Asp Gly Pro Arg Pro Ala Gly Ala Arg126512701275Leu Val Phe Val Ala Arg Gly Val Val Ala Ala Arg Pro Gly Asp128012851290
Pro Ala Ala Asn Leu Ala Gly Ala Ala Val Trp Gly Met Val Arg129513001305Thr Ala Gln Ala Glu His Pro Ala Asp Phe Thr Leu Val Asp Val131013151320Glu Pro Gly Ala Pro Phe Pro Leu Glu Ala Val Leu Ala Val Asp132513301335Glu Pro Gln Ala Ala Val Arg Asp Gly Gln Val Leu Val Gly Arg134013451350Leu Thr Gln Leu Pro Pro Pro Thr Pro Gln Asp Gly Pro Glu Asn135513601365Thr Asp Pro Phe Gly Val Thr Arg Asp Gly Thr Val Leu Val Thr137013751380Gly Gly Thr Gly Thr Leu Gly Ala Arg Thr Ala Arg His Leu Val138513901395Thr Arg His Gly Val Thr Arg Leu Leu Leu Ala Gly Arg Arg Gly140014051410Pro Asp Ala Pro Gly Ala Ala Ala Leu Arg Glu Glu Leu Thr Ala141514201425Leu Gly Ala His Val Thr Val Ala Ala Cys Asp Val Ser Asp Arg143014351440Asp Ala Leu Ala Ala Leu Leu Gly Gly Ile Pro Ala Glu His Pro144514501455Leu Thr Ala Val Val His Ala Ala Gly Val Leu Asp Asp Ala Val146014651470Val Thr Ser Leu Thr Pro Asp Arg Leu Ala Thr Val Leu Arg Ser147514801485Lys Ala Asp Ala Ala Trp His Leu His Asp Leu Thr Arg Gly Leu149014951500Gly Leu Asp Ala Phe Val Leu Tyr Ser Ser Ile Ser Gly Val Thr150515101515Ala Arg Ala Gly Gln Ala Asn Tyr Val Ala Ala Asn Leu Phe Leu152015251530Asp Thr Leu Ala Gln His Arg Ala Ala Gln Gly Leu Pro Ala Leu153515401545Ser Val Ala Trp Gly Ala Trp Asp Leu Asp Glu Gly Met Ala Gly155015551560Asp Leu Leu Thr Asp Thr Gly Arg Arg Arg Ile Arg Ser Gly Gly156515701575Ile Asp Thr Leu Pro Gly Asp Arg Ala Leu Asp Leu Leu Asp Thr158015851590Ala Leu Thr Leu Gly Glu Pro Leu Val Val Ala Thr Asn Leu Ala159516001605Ala Pro Pro Ala Thr Ala His Leu Asp Glu Val Pro Pro Leu Met161016151620
Arg Gly Leu Val Arg Pro Pro Arg Arg Thr Ala Ala Ala Thr Thr162516301635Asp Ala Pro Ala Arg Val Leu Asp Ala Ala Ser Phe Arg Glu Asn164016451650Phe Ala Asn Leu Gly Ala Gly Glu Gln Glu Thr Thr Leu Arg Glu165516601665Thr Val Val Ala Cys Ala Ala Ala Leu Leu Gly His Thr Asp Thr167016751680Ser Glu Ile Asp Pro Asp Arg Asp Phe Leu Glu Leu Gly Phe Asp168516901695Ser Leu Ile Gly Ile Glu Leu Arg Arg Lys Leu Ser Glu Ile Thr170017051710Gly Leu Gln Leu Pro Ala Ser Ile Val Tyr Asp Ser Gly Ser Pro171517201725Asn Gly Leu Thr Ala Trp Leu Arg Thr Glu Leu Gly Ala Gln Gly173017351740Ser Gly Ala Pro Gln Thr Gly Ala Ala Gly Gly Pro Thr Glu Asn174517501755Asp Ser Met Glu Arg Leu Phe Leu Asp Gly Leu Ala Gln Gly Lys176017651770Val Arg Glu Ala Gln Arg Met Leu Ala Thr Val Ala Ala Leu Arg177517801785Pro Ser Phe Glu Val Thr Ala Glu Leu Glu Asp Leu Pro Trp Pro179017951800Val Thr Leu Ala Glu Gly Pro Ala Pro Thr Arg Leu Val Cys Val180518101815Ser Ala Pro Thr Ala Asn Gly Gly Val His Gln Tyr Ala Thr Leu182018251830Ser Gly His Phe Arg Gly Arg Arg Asp Val Thr Ala Leu Pro Leu183518401845Ile Gly Phe Asn Thr Gly Glu Ala Leu Pro Ala His Ala Glu Ala185018551860Ala Ala Arg Ile Ile Ala Asp Cys Thr Leu Gln Ala Ala Asp Gly186518701875Lys Pro Phe Val Leu Val Gly His Ser Ser Gly Gly Ser Leu Ala188018851890Tyr Ala Ala Ala Gly Val Leu Glu His Thr Trp Gly lle Arg Pro189519001905Glu Ala Val Val Leu Leu Asp Thr Leu Ser Ile Gln His Lys Ser191019151920Asp Glu Gly Val Asp Tyr Asn Gly Met Met Lys Phe Asn Phe Thr192519301935Ala Val Asp Asp Ser Pro Val Arg Leu Thr Asn Ser Arg Leu Ser194019451950
Ala Met Gly Arg Trp Met Val Leu Leu Asn Ala Leu Asp Val His195519601965Pro Thr Thr Val Pro Val Leu Glu Ile Lys Cys Thr Arg Ala Leu197019751980Ile Glu Gly Val Pro Ala Pro Asp Pro Glu Arg Leu His Leu Pro198519901995Val Val Pro Asp Ala Glu Val Arg Pro Leu Asp Ser Asp His Leu200020052010Ser Leu Ile Arg Glu Asp Ser Gly Pro Ala Ala Asp Leu Met Asp201520202025Ala Trp Leu Thr Ala Leu Glu Thr Thr Ala Thr Thr Asp Ala Pro203020352040Glu Thr Ala Pro Ala Gly2045<210>6<211>7771<212>PRT<213>鏈霉菌FR-008<400>6Met Thr Thr Pro Ser Glu Lys Val Val Glu Ala Leu Arg Ala Ser Leu1 5 10 15Lys Glu Thr Glu Arg Leu Arg Arg Gln Asn Arg Asp Leu Ala Ala Ala20 25 30Ala Ser Glu Pro Val Ala Val Val Ala Met Ser Cys Arg Tyr Pro Gly35 40 45Gly Val Thr Ser Pro Glu Asp Leu Trp His Leu Val Ala Ser Gly Thr50 55 60Asp Ala Val Gly Gly Phe Pro Thr Asp Arg Gly Trp Asp Leu Asp Ala65 70 75 80Leu Arg Asp Ala Gly Val Asp Ala Arg Gly His Ser Val Ser Gln Arg85 90 95Gly Gly Phe Leu Asp Gly Val Ala Asp Phe Asp Ala Ala Phe Phe Gly100 105 110Val Ser Pro Arg Glu Ala Val Ser Met Asp Pro Gln Gln Arg Leu Leu115 120 125Leu Glu Thr Ser Trp Glu Ala Val Glu Arg Ala Gly Ile Asp Pro Arg130 135 140Ser Leu Arg Gly Ser Arg Thr Gly Val Phe Val Gly Thr Asn Gly Gln145 150 155 160Asp Tyr Ala Tyr Leu Leu Val Arg Ser Leu Ala Asp Ala Thr Gly Asp165 170 175
Ile Gly Thr Gly Ile Ala Ala Ser Ala Thr Ser Gly Arg Leu Ser Tyr180 185 190Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser195 200 205Ser Ser Leu Val Ala Leu His Ala Ala Ala His Ala Leu Arg Ala Gly210 215 220Glu Cys Gly Leu Ala Leu Ala Gly Gly Val Asn Val Met Ser Ala Pro225 230 235 240Gly Ser Leu Met Glu Phe Ser Arg Ala Gly Gly Leu Ala Gly Asp Gly245 250 255Arg Cys Lys Ala Phe Ala Asp Glu Ala Asp Gly Thr Gly Trp Ser Glu260 265 270Gly Val Gly Val Leu Leu Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn275 280 285Gly His Pro Val Leu Ala Val Leu Arg Gly Ser Ala Val Asn Gln Asp290 295 300Gly Ala Ser Asn Gly Phe Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg305 310 315 320Val Ile Gln Gln Ala Leu Ala Ala Ala Gly Leu Thr Ala Ala Asp Val325 330 335Asp Ala Val Glu Gly His Gly Thr Gly Thr Pro Leu Gly Asp Pro Ile340 345 350Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Gly Arg Pro Glu Asp355 360 365Arg Pro Leu Leu Leu Gly Ser Val Lys Ser Asn Ile Gly His Ala Gln370 375 380Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Ile Met Ala Met Arg385 390 395 400His Gly Val Val Pro Arg Ser Leu His Ala Asp His Pro Ser Arg His405 410 415Val Asp Trp Asp Ser Gly Ala Val Arg Leu Leu Ser Glu Ala Val Glu420 425 430Trp Pro Glu Thr Gly Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly435 440 445Ile Ser Gly Thr Asn Ala His Val Val Ile Glu Gln Ala Gly Pro Ala450 455 460Ala Glu Pro Glu Ser Pro Ala Glu Pro Glu Thr Val Pro Thr Val Val465 470 475 480Pro Trp Pro Val His Ala Arg Ser Glu Ala Ser Leu Asp Ala Gln Ile485 490 495Glu Ala Val Thr Ala Leu Thr Asp Ala Thr Pro Leu Asp Ile Gly His500 505 510Ser Leu Ala Thr Gly Arg Ser Leu Phe Glu His Arg Ala Val Leu Leu515 520 525
Ser Gly Leu Asp Gly Val Pro Ala Glu Ala Ala Arg Gly Arg Ala Thr530 535 540Glu Arg Ser Leu Ala Val Leu Phe Ser Gly Gln Gly Ala Gln Arg Ala545 550 555 560Glu Met Gly Arg Glu Leu Tyr Ala Arg Phe Pro Val Phe Ala Lys Ala565 570 575Leu Asp Glu Ile Thr Ala Leu Leu Asp Pro Arg Leu Asp Arg Pro Leu580 585 590Arg Glu Val Met Phe Ala Arg Arg Arg Thr Pro Glu Ala Ala Leu Leu595 600 605Asp Thr Thr Gly Tyr Thr Gln Pro Ala Leu Phe Ala Val Glu Val Ala610 615 620Leu His Arg Leu Val Glu Ser Trp Gly Val Val Pro Asp Phe Val Ala625 630 635 640Gly His Ser Ile Gly Glu Ile Ala Ala Ala His Val Ala Gly Val Phe645 650 655Ser Leu Glu Asp Ala Cys Thr Leu Val Ala Ala Arg Ala Arg Leu Met660 665 670Gln Glu Leu Pro Ala Gly Gly Ala Met Val Ala Val Gln Ala Thr Glu675 680 685Ala Glu Ala Ala Pro Arg Leu Thr Glu Gly Val Ala Leu Ala Ala Val690 695 700Asn Gly Pro Asp Ser Val Thr Leu Ala Gly Glu Glu Ala Gln Val Leu705 710 715 720Ala Leu Ala Ala Glu Phe Ala Ala Glu Gly Arg Lys Thr Gln Arg Leu725 730 735Ala Val Ser His Ala Phe His Ser Pro Leu Met Glu Pro Met Leu Ala740 745 750Glu Phe Arg Arg Val Ala Glu Ser Leu Thr Tyr His Glu Pro Val Leu755 760 765Pro Val Val Ser Asn Val Thr Gly Ala Leu Ala Asp Asp Arg Leu Leu770 775 780Cys Asp Pro Glu Tyr Trp Val Asp His Val Arg Ser Thr Val Arg Phe785 790 795 800Ala Asp Gly Val Arg Ala Leu Ala Gly Ala Gly Ala Asp Thr Phe Val805 810 815Glu Leu Gly Pro Asp Gly Ile Leu Thr Ala Leu Ala Gln Gln Cys Leu820 825 830Asp Gly Pro Asp Val Val Ala Val Pro Val Leu Arg Lys Asp Arg Ala835 840 845Glu Glu Arg Ala Leu Leu Thr Ala Leu Ala Arg Leu His Val Ala Gly850 855 860Val Asp Ile Asp Trp Ala Ala Cys Phe Glu Gly Thr Gly Ala Arg Arg865 870 875 880
Thr Glu Leu Pro Thr Tyr Arg Trp Gln His Glu Arg Tyr Trp Pro Val885 890 895Leu Met Ala Ala Ala Gly Asp Val Ser Ala Ala Gly Leu Val Ser Ala900 905 910Glu His Pro Leu Leu Gly Ala Ala Val Ser Leu Ala Gly Ser Asp Gly915 920 925Val Leu Phe Thr Gly Arg Leu Ser Ala Gln Thr His Pro Trp Leu Leu930 935 940Asp His Thr Val Gly Gly Met Val Ala Phe Pro Gly Thr Gly Phe Leu945 950 955 960Glu Leu Ala Val Arg Ala Gly Asp Gln Val Gly Cys Asp Arg Val Glu965 970 975Asp Leu Thr Leu Ala Lys Pro Leu Ile Leu Thr Glu Asn Ser Ala Ala980 985 990Val Val Gln Val His Val Gly Ala Ala Asp Ala Asp Gly Ala Arg Arg995 10001005Val Thr Val Tyr Ser Gln Ala Val Asp Asp Pro Glu Gln Arg Trp101010151020Thr Glu His Ala Ser Gly Leu Leu Thr Arg Gly Glu Arg Val Thr102510301035Gly Phe Asp Ser Thr Val Trp Pro Pro Arg Gly Ala Val Ala Ala104010451050Asp Leu Glu Gly Phe Tyr Ala Arg Thr Glu Tyr Gly Pro Val Phe105510601065Gln Gly Leu Arg Ala Val Trp Thr Arg Asp Asp Glu Ala Phe Val107010751080Glu Val Ala Leu Pro Pro Gln Val Asp Asp Ala Glu Tyr Tyr Gly108510901095Met His Pro Ala Leu Leu Asp Ala Ala Val Gln Ser Val Gly Phe110011051110Ala Gly Leu Gly Asp Gly Lys Lys Leu Val Pro Phe Ser Trp Ser111511201125Gly Val Ser Leu His Ala Gly Gly Ala Ser Val Val Arg Val Arg113011351140Val Ala Arg Val Gly Glu Asp Thr Val Ser Ile Ala Ala Val Asp114511501155Val Glu Gly Ala Pro Val Leu Ser Ala Asp Ser Leu Ile Leu Arg116011651170Ala Pro Ser Ala Leu Arg Ala Pro Thr Leu His Ser Ser Glu Gln117511801185Asp Gly Leu Leu Arg Leu Glu Trp Val Ala Ala Pro Glu Gly Gly119011951200Ala Ala Pro Val Arg Ala Val Thr Leu Gly Thr Asp Arg Ala Trp120512101215
Pro Gly Pro Leu Ala Ser Leu Ala Asp Ala Ala Ala Val Thr Pro122012251230Ala Pro Asp Leu Val Leu Val Pro Leu Glu Gly Val Glu Asp Asp123512401245Thr Pro Thr Ala Val His Ala Ala Thr Ala Arg Thr Leu Ala Leu125012551260Ile Gln Glu Trp Leu Glu Pro Ala Thr Ser Ala Ala Gly Arg Leu126512701275Val Phe Val Thr Arg Gly Ala Val Ala Thr Gly Thr Asp Thr Gly128012851290Val Ser Asp Leu Ala Ala Ala Ala Val His Gly Leu Val Arg Ser129513001305Ala Glu Ala Glu Asn Pro Gly Arg Phe Ala Leu Leu Asp Leu Asp131013151320Ala Gly Thr Ala Ala Pro Ser Gly Glu Leu Leu Ala Gln Leu Pro132513301335Pro Leu Leu Ala Ala Gly Asp Thr Gln Phe Ala Val Arg Asp Glu134013451350Ala Val Leu Val Ala Arg Leu Ala Arg Leu Ser Thr Gly Ala Ser135513601365Leu Leu Pro Val Pro Gly Leu Pro Trp Arg Leu Asp Thr Thr Val137013751380Pro Gly Ser Ile Asp Gly Leu Ser Leu Val Pro Ala Pro Glu Ala138513901395Leu Asp Glu Pro Glu Gly Arg Ala Val Arg Val Glu Val Arg Ala140014051410Ala Gly Leu Asn Phe Arg Asp Val Leu Asn Ala Leu Gly Met Tyr141514201425Pro Gly Glu Ala Gly Leu Leu Gly Ser Glu Ala Val Gly Leu Val143014351440Thr Ala Val Gly Pro Asp Val Thr Gly Ile Ala Val Gly Asp Arg144514501455Val Thr Gly Met Ile Pro Gly Gly Leu Ala Asp Thr Val Leu Val146014651470Asp Glu Arg Tyr Val Thr Arg Val Pro Glu Glu Trp Ser Asp Glu147514801485Asp Ala Ala Ser Val Pro Leu Val Phe Leu Thr Ala Leu Tyr Ala149014951500Phe Arg Asp Leu Ala Ser Val Gln Ala Gly Glu Arg Val Leu Ile150515101515His Ala Gly Ala Gly Gly Val Gly Met Ala Ala Ile Gln Leu Ala152015251530Arg His Met Gly Ala Glu Val Phe Ala Thr Ala Ser Glu Pro Lys153515401545
Trp Glu Thr Leu Arg Gly Leu Gly Leu Asp Asp Ala His Ile Ala155015551560Ser Ser Arg Asp Leu Gly Phe Glu Glu Lys Phe Arg Glu Val Thr156515701575Gly Gly Ala Gly Met Asp Val Val Leu Asn Ala Leu Ala Gly Asp158015851590Phe Val Asp Ala Ser Leu Arg Ile Thr Ala Pro Gly Gly Arg Phe159516001605Leu Glu Met Gly Lys Thr Asp Ile Arg Asp Pro Gln Thr Thr Gly161016151620Glu Val Arg Tyr Arg Ala Phe Asp Leu Gly Glu Ala Ala Pro Glu162516301635Arg Ile Gly Glu Met Leu Thr Glu Leu Leu Gly Leu Phe Ala Glu164016451650Gly Ala Leu Gln Pro Leu Pro Val Arg Ala Trp Asp Val Arg Arg165516601665Ala Arg Glu Ala Phe Arg Phe Met Ser Gln Ala Arg His Val Gly167016751680Lys Ile Val Leu Thr Met Pro Pro Arg Trp Asn Pro Glu Gly Thr168516901695Val Leu Val Thr Gly Gly Thr Gly Gly Leu Gly Arg Glu Val Ala170017051710Arg His Leu Val Ser Ala Arg Gly Val Arg Arg Leu Leu Leu Val171517201725Ser Arg Arg Gly Pro Ala Ala Glu Gly Val Asp Ala Phe Arg Glu173017351740Glu Leu Ala Gly Leu Gly Ala His Val Asp Val Val Ala Cys Asp174517501755Val Ala Asp Arg Ala Ala Val Ala Gly Leu Leu Gly Ser Val Pro176017651770Val Gly His Pro Leu Thr Ala Val Val His Thr Ala Gly Val Leu177517801785Asp Asp Gly Val Val Thr Gly Leu Ser Pro Glu Arg Leu Ser Gly179017951800Val Leu Arg Pro Lys Val Asp Ala Ala Trp His Leu His Glu Leu180518101815Thr Arg Asp Met Gly Leu Ala Gly Phe Val Met Phe Ser Ser Val182018251830Ser Gly Val Met Gly Ser Ala Gly Gln Ala Asn Tyr Ala Ala Ala183518401845Asn Val Phe Met Asp Ala Leu Ala Gln Tyr Arg Arg Ala Glu Gly185018551860Leu Ala Gly Leu Ser Leu Ala Trp Gly Ala Trp Glu Gln Thr Ser186518701875
Gly Met Thr Gly Thr Leu Thr Glu Ala Asp Met Gln Arg Val Thr188018851890Ala Ser Gly Ala Ala Pro Leu Thr Val Glu Gln Gly Leu Ala Leu189519001905Leu Asp Ala Ala Thr Gly Ser Asp Glu Pro Leu Val Val Pro Ile191019151920Gly Ala Pro Ser Gly Asp Gln Arg Val Leu Gly Leu Val Pro Pro192519301935Leu Leu Arg Asn Leu Val Arg Gly Thr Arg Arg Ser Ala Ala Thr194019451950Ala Ala Gly Gly Ala Ser Thr Ala Ala Asp Leu Ala Arg Arg Leu195519601965Leu Ala Leu Pro Ala Glu Asp Arg Val Arg His Ala Val Glu Leu197019751980Val Arg Thr Glu Ala Ala Ala Val Leu Gly His Ala Ser Ala Arg198519901995Ala Val Glu Pro Gly Arg Glu Phe Arg Glu Leu Gly Phe Asp Ser200020052010Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Thr Thr Val Thr Gly201520202025Leu Arg Leu Thr Ala Thr Leu Val Phe Asp Tyr Pro Thr Pro His203020352040Gly Leu Ala Glu His Leu Val Ala Glu Leu Leu Asp Glu His Gly204520502055Glu Thr Gly Thr Pro Val Val Ala Ala Asp Val Ala Asp Asp Pro206020652070Val Val Ile Val Gly Met Ala Cys Arg Met Pro Gly Gly Ile Glu207520802085Thr Pro Asp Asp Leu Trp Arg Met Leu Ala Asp Gly Glu Asp Arg209020952100Ile Thr Gly Phe Pro Thr Asp Arg Gly Trp Asp Leu Asp Ala Leu210521102115Phe Gly Gly Gly Gly Asp Asn Arg Gly Val Ser Ala Thr Arg Arg212021252130Gly Gly Phe Leu His Asp Val Gly Gly Phe Asp Ala Gly Phe Phe213521402145Gly Ile Ser Pro Arg Glu Ala Met Ala Met Asp Pro Gln Gln Arg215021552160Leu Leu Leu Glu Thr Ser Trp Glu Ala Leu Glu Arg Ser Gly Ile216521702175Asp Pro Thr Gly Leu Arg Ser Ser Ala Thr Gly Ile Phe Val Gly218021852190Thr Thr Gly Gln Asp Tyr Ala Asn Leu Val Met Thr Ser Arg Glu219522002205
Asp Val Glu Gly His Ala Ser Thr Gly Leu Ala Thr Ser Val Ile221022152220Ser Gly Arg Val Ser Tyr Ala Leu Gly Leu Glu Gly Pro Ala Leu222522302235Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His Leu224022452250Ala Ala Gln Ser Leu Arg Ser Gly Glu Ser Ser Leu Ala Leu Ala225522602265Gly Gly Val Thr Val Leu Ser Thr Pro Met Asn Phe Phe Gly Phe227022752280Thr Arg Gln Gly Gly Leu Ala Gly Asp Gly Leu Cys Lys Ala Phe228522902295Ala Asp Ala Ala Asp Gly Thr Gly Trp Ser Glu Gly Val Gly Met230023052310Leu Val Leu Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Asp231523202325Val Leu Ala Val Val Arg Gly Ser Ala Ile Asn Gln Asp Gly Ala233023352340Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val234523502355Ile Arg Gln Ala Leu Ala Thr Ala Gly Leu Ser Ala Asp Glu Val236023652370Asp Ala Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro237523802385Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg Pro239023952400Glu Asp Arg Pro Leu Leu Leu Gly Ser Ile Lys Ser Asn Ile Gly240524102415His Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val242024252430Leu Ala Met Arg His Gly Ala Leu Pro Arg Ser Leu His Ile Asp243524402445Gln Pro Ser Thr His Val Asp Trp Glu Ala Gly Ala Val Arg Leu245024552460Leu Thr Glu Thr Thr Gly Trp Pro Glu Thr Gly Arg Pro Trp Arg246524702475Ala Gly Val Ser Ser Phe Gly Leu Ser Gly Thr Asn Ala His Val248024852490Ile Leu Glu Gln Pro Glu Pro Glu Thr Ala Asp Thr Glu Ser Pro249525002505Glu Pro Gln Ala Glu Pro Glu Ala Leu Ala Arg Val Val Pro Trp251025152520Pro Val Ser Ala Arg Thr Glu Glu Ala Leu Pro Gly Gln Leu Ala252525302535
Arg Ile Thr Ala Leu Asp Ala Ala Pro Leu Asp Ile Gly His Ser254025452550Leu Ala Thr Gly Arg Ala Ser Phe Asp His Arg Val Val Leu Leu255525602565Ala Gly Ala Asp Gly Glu Asp Glu Pro Val Glu Val Ala Arg Gly257025752580Arg Ala Val Glu Arg Arg Leu Ala Val Leu Phe Ser Gly Gln Gly258525902595Ser Gln Arg Ala Gly Met Gly Arg Glu Leu Tyr Gly Arg Phe Pro260026052610Val Phe Thr Lys Ala Leu Asp Glu Val Leu Ala Leu Leu Asp Pro261526202625Lys Leu Asp Arg Pro Leu Arg Glu Val Leu Phe Ala Glu Glu Gly263026352640Ser Glu Thr Ala Ala Leu Leu Asp Thr Thr Gly Tyr Thr Gln Pro264526502655Ala Leu Phe Ala Val Glu Val Ala Leu Tyr Arg Leu Val Glu Ser266026652670Phe Gly Val Val Pro Glu Phe Val Ala Gly His Ser Val Gly Glu267526802685Ile Ser Ala Ala His Ile Ala Gly Val Leu Ser Leu Glu Asp Ala269026952700Cys Thr Leu Val Ala Ala Arg Ala Arg Leu Met Gln Glu Leu Pro270527102715Ala Gly Gly Ala Met Val Ala Val Gln Ala Thr Glu Ala Glu Thr272027252730Ala Gly Arg Leu Thr Gly Gly Leu Ser Leu Ala Ala Val Asn Gly273527402745Pro Asp Ser Val Val Ile Ala Gly Pro Asp His Glu Val Asp Ala275027552760Leu Ala Ser Glu Phe Ala Ala Glu Gly Arg Lys Val Gln Arg Leu276527702775Ala Val Ser His Ala Phe His Ser Ala Leu Met Glu Pro Met Leu278027852790Asp Ala Phe His Asp Val Ala Lys Gly Leu Gly Tyr Gly Glu Pro279528002805Arg Ile Pro Val Val Ser Asp Val Thr Gly Thr Leu Ala Glu Pro281028152820Gly Gln Leu Ser Arg Pro Glu Tyr Trp Val Glu His Val Arg Ser282528302835Thr Val Arg Phe Ala Asp Ala Val His Ala Leu Asp Gln Ala Gly284028452850Ala Asn Ala Tyr Leu Glu Ile Gly Pro Gly Gly Val Leu Thr Val285528602865
Leu Ala Gln Gln Thr Leu Asp Ala Ala Gly Thr Ala Ser Asp Ser287028752880Val Thr Ala Pro Ala Leu Arg Lys Asp Arg Ala Glu Glu Pro Ala288528902895Leu Leu Thr Ala Leu Ala Thr Leu His Val Thr Gly Val Pro Val290029052910Asp Trp Ala Gly Cys Phe Glu Gly Thr Gly Ala Arg Arg Val Glu291529202925Leu Pro Thr Tyr Ala Phe Gln His Glu Arg Tyr Trp Pro Arg Pro293029352940Ser Ala His Gly Gly Asp Val Thr Gly Ala Gly Leu Ser Pro Ala294529502955Ala His Pro Leu Leu Gly Ala Ala Thr Gly Leu Ala Ala Ser Glu296029652970Gly Val Leu Phe Thr Gly Arg Leu Ser Leu Thr Thr His Pro Trp297529802985Leu Ala Asp His Thr Val Gly Gly Gly Met Val Leu Phe Pro Ala299029953000Thr Gly Phe Leu Glu Leu Ala Val Arg Ala Ala Asp Glu Val Gly300530103015Cys Asp Arg Val Glu Glu Phe Thr Leu Ala Thr Pro Leu Leu Leu302030253030Pro Ala Asp Thr Ala Val Val Val Gln Val Trp Ala Gly Ala Pro303530403045Asp Glu Ser Gly Ala Arg Thr Val Ser Leu Tyr Ser Arg Ser Ala305030553060Asp Val Pro Asp Ala Thr Trp Thr Gln His Ala Thr Gly Val Leu306530703075Thr Ser Gly Ala Val Thr Leu Pro Ala Val Ala Glu Ile Trp Pro308030853090Pro Lys Gly Ala Val Ala Val Gly Leu Glu Asp Phe Tyr Asp Arg309531003105Thr Glu Tyr Gly Pro Val Phe Arg Thr Ile Arg Ala Val Trp Lys311031153120Arg Gly Asp Glu Ala Phe Val Glu Ala Ala Leu Pro Pro Gln Ala312531303135Asp Asp Ala Glu Tyr Tyr Gly Met His Pro Ala Leu Leu Asp Ala314031453150Ala Val Gln Ser Val Gly Phe Ala Gly Leu Asp Asp Glu His Gln315531603165Leu Leu Pro Phe Leu Trp Ala Gly Val Ser Leu His Ala Gly Gly317031753180Ala Ser Val Val Arg Phe Arg Val Ala Arg Thr Gly Glu Asp Ser318531903195
Val Ser Ile Ala Ala Val Asp Val Glu Gly Ala Pro Val Leu Ser320032053210Ala Glu Ser Leu Val Leu Arg Val Pro Ala Gly Asp His Ala Pro321532203225Ala Ala Arg Arg Thr Glu Leu Asp Ser Leu Leu Arg Leu Glu Trp323032353240Thr Ala Gly Pro Glu Thr Gly Asp Ala Pro Ala Ala Arg His Thr324532503255Thr Ala Gly Ala Leu Gly Thr Gly Gly Ala Ala Ala Thr Leu Ala326032653270Pro Leu Thr Gly Glu Glu Asp Leu Val Leu Val Glu Val Pro Ala327532803285Pro Ala Glu Gly Ala Asp Val Pro Ala Ala Thr His Glu Arg Ala329032953300Ala Gln Ala Leu Ala Leu Ala Gln Glu Trp Leu Ala Asp Glu Arg330533103315Phe Ala Asp Thr Arg Leu Val Phe Val Thr Arg Gly Ala Val Cys332033253330Ala Arg Pro Gly Glu Arg Val Asp Asp Leu Pro Ala Ala Ala Val333533403345Trp Gly Leu Val Arg Ala Ala Gln Ser Glu Asn Pro Thr Arg Phe335033553360Ala Leu Leu Asp Leu Asp Asp Thr Thr Pro Val Glu Thr Ala Leu336533703375Pro Gln Leu Leu Gly Pro Leu Ser Gly Gly Asp Ala Gln Phe Val338033853390Val Arg Glu Gly Ala Val Leu Val Gly Arg Leu Asp Arg Ala Val339534003405Thr Ala Pro Ser Leu Leu Pro Pro Ala Asp Gly Pro Trp Arg Leu341034153420Asp Ser Pro Ser Arg Gly Ser Leu Asp Ala Leu Thr Leu Arg Pro342534303435Cys Pro Glu Glu Leu Ala Glu Pro Gln Glu Arg Gln Val Arg Val344034453450Glu Val Arg Ala Ala Gly Leu Asn Phe Arg Asp Val Leu Asn Ala345534603465Leu Gly Met Tyr Pro Gly Glu Ala Gly Leu Leu Gly Ala Glu Ala347034753480Ala Gly Phe Val Thr Ala Val Gly Pro Glu Val Thr Gly Leu Ser348534903495Val Gly Asp Arg Val Met Gly Met Val Pro Gly Gly Leu Ala Thr350035053510Glu Thr Leu Ile Asp Glu Arg Phe Leu Thr Arg Val Pro Glu Glu351535203525
Trp Ser Asp Glu Asp Ala Ala Ser Val Pro Leu Val Phe Leu Thr353035353540Ala Tyr Tyr Ala Leu Thr Glu Leu Ala Gly Leu Ser Ala Gly Glu354535503555Arg Val Leu Ile His Ala Gly Ala Gly Gly Val Gly Met Ala Ala356035653570Ile Gln Leu Ala Arg His Met Gly Ala Glu Val Phe Ala Thr Ala357535803585Ser Glu Pro Lys Trp Glu Thr Leu Arg Gly Leu Gly Leu Asp Asp359035953600Ala His Ile Ala Ser Ser Arg Asp Leu Gly Phe Glu Glu Lys Phe360536103615Arg Glu Glu Thr Gly Gly Ala Gly Met Asp Val Val Leu Asn Ala362036253630Leu Ala Gly Asp Phe Val Asp Ala Ser Leu Arg Ile Thr Ala Ser363536403645Gly Gly Arg Phe Leu Glu Met Gly Lys Thr Asp Ile Arg Asp Pro365036553660His Ala Val Gly Asp Val Arg Tyr Arg Ala Phe Asp Leu Gly Glu366536703675Ala Gly Pro Asp Arg Thr Gly Glu Met Leu Ser Glu Leu Leu Gly368036853690Leu Phe Ala Glu Gly Ala Leu Arg Pro Leu Pro Val Arg Ala Trp369537003705Asp Val Arg Arg Ala Arg Glu Ala Phe Arg Phe Met Ser Gln Ala371037153720Arg His Val Gly Lys Ile Val Leu Thr Met Pro Pro Arg Trp Asn372537303735Pro Glu Gly Thr Val Leu Val Thr Gly Gly Thr Gly Gly Leu Gly374037453750Arg Glu Val Ala Arg His Leu Val Ser Ala Arg Gly Val Arg Arg375537603765Leu Leu Leu Val Ser Arg Arg Gly Pro Ala Ala Glu Gly Val Asp377037753780Ala Phe Arg Glu Glu Leu Ala Gly Leu Gly Ala His Val Asp Val378537903795Val Ala Cys Asp Val Ala Asp Arg Ala Ala Val Ala Gly Leu Leu380038053810Ala Ser Val Pro Val Gly His Pro Leu Thr Ala Val Val His Thr381538203825Ala Gly Val Leu Asp Asp Gly Val Val Thr Gly Leu Ser Pro Glu383038353840Arg Leu Ser Gly Val Leu Arg Pro Lys Val Asp Ala Ala Trp His384538503855
Leu His Glu Leu Thr Arg Asp Met Gly Leu Ala Gly Phe Val Met386038653870Phe Ser Ser Val Ser Gly Val Met Gly Ser Ala Gly Gln Gly Asn387538803885Tyr Ala Ala Ala Asn Val Phe Met Asp Ala Leu Ala Gln Tyr Arg389038953900Arg Ala Glu Gly Leu Ala Gly Leu Ser Leu Ala Trp Gly Ala Trp390539103915Glu Gln Thr Ser Gly Met Thr Gly Thr Leu Thr Asp Ala Gly Val392039253930Arg Arg Met Ser Ala Ser Ala Ala Pro Ala Leu Thr Val Glu Gln393539403945Gly Leu Ala Leu Leu Asp Ala Ala Val Val Ser Asp Glu Pro His395039553960Leu Val Pro Leu Gly Ala Ser Gly Ser Thr Arg Met Pro Gly Glu396539703975Val Pro Pro Leu Leu Arg Asn Leu Val Arg Gly Thr Arg Arg Ala398039853990Ala Ala Thr Ala Val Gly Gly Ala Gly Thr Ala Asp Glu Leu Thr399540004005Arg Arg Leu Leu Asp Leu Arg Glu Asp Glu Arg Leu Arg Phe Ala401040154020Val Asn Leu Ile Arg Ala Glu Ala Ala Ser Val Leu Gly His Ser402540304035Ser Ala Gln Ala Ile Asp Ala Glu Arg Asp Phe His Asp Leu Gly404040454050Phe Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Thr Gly405540604065Ala Thr Gly Leu Arg Leu Pro Ala Thr Leu Val Phe Asp His Pro407040754080Thr Pro Gly Val Leu Ala Glu His Leu Ile Ala Ala Leu Leu Asp408540904095Glu Gln Arg Ala Thr Gly Ala Thr Ala Pro Thr Pro Val Ala Ala410041054110Ala Leu Ala Glu Asp Pro Val Val Ile Val Gly Met Ala Cys Arg411541204125Met Pro Gly Gly Val Ser Ser Pro Glu Glu Leu Trp Gln Leu Val413041354140Leu Asp Gly Gln Glu Gly Ile Ser Ala Phe Pro Thr Asp Arg Gly414541504155Trp Asp Leu Asp Thr Leu Gln Arg Gly Gly Glu Ser Gly His Gly416041654170Arg Ser Ala Thr Ser Glu Gly Gly Phe Leu Tyr Asp Val Ala Asp417541804185
Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala419041954200Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Thr Trp Glu Ala420542104215Phe Glu Arg Ala Gly Ile Thr Pro Thr Glu Val Arg Gly Ser Arg422042254230Thr Gly Val Phe Val Gly Thr Ser Gly Gln Asp Tyr Thr Thr Leu423542404245Val Met Asn Ser Arg Gln Asp Ala Glu Gly His Ala Pro Thr Gly425042554260Leu Ala Thr Ser Val Ile Ser Gly Arg Leu Ser Tyr Thr Phe Gly426542704275Leu Glu Gly Pro Ala Val Thr Ile Asp Thr Ala Cys Ser Ser Ser428042854290Leu Val Ala Leu His Trp Ala Ala His Ala Leu Arg Ser Gly Glu429543004305Ala Asp Leu Ala Leu Ala Gly Gly Val Thr Val Met Ser Thr Ala431043154320Met Gly Tyr Ala Gly Phe Thr Arg Gln Gly Gly Leu Ala Thr Asp432543304335Gly Arg Cys Lys Ala Phe Ala Asp Ala Ala Asp Gly Thr Gly Trp434043454350Ser Glu Gly Val Gly Met Leu Val Val Glu Arg Leu Ser Asp Ala435543604365Arg Arg Asn Gly His Pro Val Leu Ala Val Leu Arg Gly Ser Ala437043754380Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly438543904395Pro Ser Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Ser Ala Gly440044054410Leu Ser Thr Asp Glu Val Asp Ala Val Glu Ala His Gly Thr Gly441544204425Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr443044354440Tyr Gly Gln Asp Arg Pro Gly Asp Arg Pro Leu Leu Leu Gly Ser444544504455Ile Lys Ser Asn Ile Gly His Ala Gln Ala Ala Ala Gly Val Ala446044654470Gly Val Ile Lys Thr Val Met Ala Ile Arg His Gly Val Leu Pro447544804485Arg Ser Leu His Ile Asp Arg Pro Ser Thr His Val Asp Trp Thr449044954500Glu Gly Asp Val Arg Leu Leu Thr Glu Thr Thr Glu Trp Pro Glu450545104515
Thr Gly Arg Pro Arg Arg Ala Ala Val Ser Ser Phe Gly Ile Ser452045254530Gly Thr Asn Ala His Thr Ile Ile Glu Gln Ala Pro Glu Ile Val453545404545Glu Ala Thr Pro Ala Ala Ala Ser Ala Pro Ala Pro Ala Ala Val455045554560Pro Trp Pro Val Ser Ala Lys Ser Pro Glu Ala Leu Asp Ala Gln456545704575Leu Ala Arg Ile Ala Thr Val Thr Gly Ala Thr Pro Leu Asp Ile458045854590Gly His Ser Leu Ala Thr Gly Arg Ala Ser Phe Glu Tyr Arg Ala459546004605Val Leu Leu Ala Gly Thr Gly Ala Glu Asp Glu Pro Val Glu Val461046154620Ala Arg Gly Arg Ala Val Glu Arg Arg Leu Ala Val Leu Phe Pro462546304635Gly Gln Gly Ser Gln Arg Ala Gly Met Gly Arg Glu Leu Tyr Glu464046454650Arg Tyr Pro Val Phe Ala Lys Ala Leu Asp Glu Ile Thr Ala Leu465546604665Leu Asp Pro Glu Leu Asp Arg Pro Leu Arg Glu Val Leu Phe Ala467046754680Glu Glu Gly Ser Glu Thr Ala Ala Leu Leu Asp Thr Thr Gly Tyr468546904695Thr Gln Pro Ala Leu Phe Ala Val Glu Val Ala Leu His Arg Leu470047054710Leu Glu Ser Gln Gly Val Ser Pro Glu Tyr Val Ala Gly His Ser471547204725Val Gly Glu Ile Ala Ala Ala His Val Ala Gly Val Phe Ser Leu473047354740Glu Asp Ala Cys Arg Leu Val Ala Ala Arg Ala Arg Leu Met Gln474547504755Glu Leu Pro Ala Gly Gly Ala Met Ala Ala Val Gln Ala Thr Glu476047654770Ala Glu Ile Ala Pro Arg Leu Thr Glu Gly Leu Ser Leu Ala Ala477547804785Val Asn Gly Pro Asp Ser Val Val Val Ser Gly Pro Glu Ser Glu479047954800Val Thr Ala Leu Ala Ala Glu Phe Val Ala Glu Gly Arg Lys Thr480548104815Gln Arg Leu Gln Val 5er His Ala Phe His Ser Ala Leu Thr Glu482048254830Pro Met Leu Asp Ala Phe Arg Glu Val Ala Arg Ser Leu Ala Tyr483548404845
Ala Glu Pro Arg Leu Pro Val Val Ser Asn Val Thr Gly Ala Leu485048554860Ala Ala Pro Gly Gln Leu Thr Asp Pro Glu Tyr Trp Val Arg His486548704875Val Arg Glu Thr Val Arg Phe Ala Asp Gly Val Arg Ala Leu Ala488048854890Glu Ala Gly Ala Asp Ala Tyr Leu Glu Ala Gly Pro Gly Gly Val489549004905Leu Thr Ala Leu Ala Arg Gln Thr Leu Gly Thr Asp Ser Glu Ala491049154920Val Thr Ala Pro Ala Leu Arg Lys Asp Arg Ala Glu Glu Pro Ala492549304935Leu Leu Thr Ala Leu Ala Thr Leu His Val Thr Gly Val Pro Val494049454950Asp Trp Ala Ala Cys Phe Glu Gly Thr Gly Ala Arg Arg Val Asp495549604965Leu Pro Thr Tyr Ala Phe Gln Arg Val Arg His Trp Pro Asp Thr497049754980Arg Arg Pro Gly Thr Gly Gly Gly Ser Ser Asp Pro Leu Asp Gly498549904995Ala Phe Trp Thr Ala Val Glu Gly Glu Asp Leu Thr Arg Leu Ala500050055010Thr Asp Leu Ala Val Asp Thr Glu Ala Leu Gly Ala Val Leu Pro501550205025Ala Leu Ser Thr Trp Arg Arg Arg Arg Arg Asp Gln Ala Met Val503050355040Asp Ser Asn Arg His His Glu Thr Trp Lys Pro Leu Ser Leu Pro504550505055Pro Gly Ala Pro Ala Pro Ala Gly Thr Trp Leu Ala Val Val Pro506050655070Ala Ala Tyr Gly Glu Asp Pro Trp Thr Ala Ala Val Leu Asp Ala507550805085Val Gly Thr Asp Val Val Arg Leu Thr Val Asp Thr Val Glu Arg509050955100His Ala Leu Ala Asp Arg Leu Arg Ala Leu Ser Ala Asp Gly Ala510551105115Ala Leu Ala Gly Val Val Ser Leu Leu Ala Leu Ala Asp Thr Pro512051255130Glu Ala Ala Val Ser Gly Asp Pro Ala Gly Ala Pro Ala Ala Pro513551405145Thr Ala Val Leu Phe Gln Ala Leu Leu Asp Ala Gly Val Asp Ala515051555160Pro Leu Trp Cys Leu Thr Arg Gly Ala Val Ala Val Ala Gly Ser516551705175
Glu Ala Val Thr Ala Pro Ala Gln Ala Ala Val Trp Gly Leu Gly518051855190Arg Val Ala Ala Leu Glu His Pro Ala Val Trp Gly Gly Leu Val519552005205Asp Leu Pro Ser Ala Trp Asp Glu Arg Ala Ala Arg Arg Phe Ala521052155220Ala Val Leu Ala Gly His Asp Gly Glu Asp Gln Val Ala Val Arg522552305235Ser Ser Ala Ala Phe Ala Arg Arg Leu Val Pro Ala Pro Ala Ala524052455250Glu Pro Gly Asp Gly Trp Gln Pro Arg Gly Thr Val Leu Val Thr525552605265Gly Gly Thr Gly Gly Arg Gly Ala His Val Ala Arg Trp Leu Ala527052755280Gly Ala Gly Ala Ala His Leu Val Leu Leu Gly Arg Arg Gly Pro528552905295Asp Ala Pro Gly Ala Asp Ala Leu Arg Ala Glu Leu Glu Ala Ala530053055310Gly Ala Arg Val Thr Leu Val Ala Cys Asp Ala Ala Asp Arg Asp531553205325Ala Leu Ala Ala Val Leu Asp Gly Ile Pro Ala Asp Thr Pro Leu533053355340Ser Ala Val Val His Ala Ala Ala Val Val Asp Asp Gly Val Leu534553505355Asp Asp Leu Thr Pro Glu Arg Phe Ala Ala Pro His His Ala Arg536053655370Thr Ala Pro Ala Leu His Leu Asp Glu Leu Thr Arg Gly Leu Asp537553805385Leu Asp Val Phe Ala Leu Cys Ser Ser Val Ala Gly Thr Val Gly539053955400Thr Ala Gly Arg Ala Asn Leu Ala Ala Ala Thr Ala Val Leu Asp540554105415Ala Leu Ala Arg Ser Arg Arg Ser Glu Gly Leu Pro Ala Thr Ser542054255430Met Ala Trp Gly Ala Trp Ile Gly Asp Ala Asp Glu Thr Gly Gly543554405445Asp Asp Ala Pro Ala Ala Pro Arg Ala Gly Ala Gly His Pro Ala545054555460Val His Pro Asp Leu Ala Leu Ala Ala Leu Arg Gln Ala Val Thr546554705475Arg Pro Glu Ala Ala Pro Val Leu Phe Asp Pro Arg Gln Pro Gln548054855490Val Leu Asp Gly Leu Ile Gly Met Arg Gly Asn Ala Leu Leu Arg549555005505
Asp Leu Pro Asp Ala Arg Gln Ala Leu Ala Asp Ala Glu Thr Thr551055155520Arg Asp Arg Ala Arg Thr Ala Ala Ser Gly Leu Ala Glu Arg Leu552555305535Arg Gly Leu Pro Ala Asp Glu Arg Thr Asp Ser Val Thr Glu Leu554055455550Val Arg Thr His Ala Ala Ala Val Leu Gly His Pro Gly Pro Glu555555605565Ala Val Ala Ala Asp Arg Asn Phe Arg Asp Leu Gly Phe Asp Ser557055755580Leu Thr Ala Ile Glu Leu Pro Asn Arg Ile Ala Leu Ala Thr Gly558555905595Leu Arg Leu Pro Ala Thr Thr Val Tyr Asp Tyr Pro Thr Ala Gln560056055610Ala Leu Ala Glu His Leu Leu Ala Glu Leu Leu Gly Glu Gln Asp561556205625Thr Pro Gly Ser Gly Arg Ala Thr Pro Ala Ala Leu Ala Glu Asp563056355640Pro Val Val Ile Val Gly Met Ala Cys Arg Leu Pro Gly Gly Val564556505655Arg Ser Pro Gln Asp Leu Trp Ala Met Val Ser Glu Gly Arg Asp566056655670Gly Ile Glu Ala Phe Pro Glu Asp Arg Gly Trp Asp Leu Ala Thr567556805685Leu Thr Thr Gly Gly Ala Asp Gly Arg Gly Arg Ser Ala Thr Leu569056955700Arg Gly Gly Phe Leu Ser Gly Ala Ala Asp Phe Asp Ala Ala Phe570557105715Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln572057255730Arg Leu Leu Leu Glu Thr Thr Trp Glu Ala Phe Glu Arg Ser Gly573557405745Ile Asp Ala Asp Gln Leu Arg Gly Ser Arg Thr Gly Val Phe Val575057555760Gly Thr Asn Gly Gln Asp Tyr Ser Thr Leu Val Met Asn Ser Arg576557705775Glu Asp Leu Glu Gly His Ala Gly Thr Gly Leu Ala Ala Ser Val578057855790Val Ser Gly Arg Leu Ala Tyr Thr Phe Gly Leu Glu Gly Pro Ala579558005805Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His581058155820Trp Ala Met Gln Ala Leu Arg Ala Gly Glu Cys Asp Leu Ala Val582558305835
Ala Gly Gly Ile Thr Met Met Ser Thr Pro Ser Ala Phe Ser Gly584058455850Phe Thr Leu Gln Asn Gly Leu Ala Val Asp Gly Arg Cys Lys Ala585558605865Tyr Ala Asp Ala Ala Asp Gly Thr Gly Trp Ser Glu Gly Val Gly587058755880Leu Ile Val Val Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His588558905895Glu Val Leu Ala Val Val Arg Gly Ser Ala Ile Asn Gln Asp Gly590059055910Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg591559205925Val Ile Arg Gln Ala Leu Ala Gly Gly Gly Leu Thr Pro Ser Asp593059355940Ile Asp Ala Val Glu Gly His Gly Thr Gly Thr Pro Leu Gly Asp594559505955Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg596059655970Pro Ala Asp Arg Pro Leu Leu Leu Gly Ser Ile Lys Ser Asn Ile597559805985Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Ile Ile Lys Thr599059956000Val Met Ala Met Arg His Gly Val Leu Pro Arg Thr Leu His Val600560106015Asp Arg Pro Ser Thr His Val Asp Trp Glu Ser Gly Ala Ile Arg602060256030Leu Leu Thr Glu Glu Ser Ala Trp Pro Asp Ser Gly Arg Pro Trp603560406045Arg Ala Gly Ile Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His605060556060Thr Ile Ile Glu Gln Ala Pro Ala Pro Glu Pro Ala Glu Pro Gly606560706075Ala Ala Leu Leu Pro Gly Pro Thr Pro Thr Val Val Pro Trp Pro608060856090Val Ser Ala Lys Ser Glu Glu Ala Leu Asp Gly Gln Ile Ala Ala609561006105Val Thr Ala Leu Asp Gly Ala Ala Pro Leu Asp Val Gly His Ser611061156120Leu Ala Thr Gly Arg Ala Arg Phe Asp His Arg Ala Val Leu Leu612561306135Ala Arg Pro Gly Thr Thr Pro Trp Glu Ala Ala Arg Gly Arg Ala614061456150Ala Val Arg Ser Leu Ala Leu Leu Phe Ser Gly Gln Gly Ser Gln615561606165
Arg Ala Gly Met Gly Arg Glu Leu Tyr Asp Arg Phe Pro Val Phe617061756180Ala Glu Ala Leu Asp Ala Val Leu Ala Arg Leu Asp Thr Gly Leu618561906195Thr Pro Ser Leu Arg Glu Val Leu Phe Ala Glu Glu Gly Ser Gly620062056210Thr Ala Ala Leu Leu Asp Thr Thr Gly Tyr Thr Gln Pro Ala Leu621562206225Phe Ala Val Glu Val Ala Leu Tyr Arg Leu Val Glu Ser Trp Gly623062356240Val Arg Pro Gly His Val Ala Gly His Ser Val Gly Glu Ile Ala624562506255Ala Ala His Val Ala Gly Val Phe Ser Leu Glu Asp Ala Cys Arg626062656270Leu Val Ala Ala Arg Ala Arg Leu Met Gln Glu Leu Pro Ser Gly627562806285Gly Ala Met Val Ala Val Arg Ala Thr Glu Ala Glu Val Val Pro629062956300Arg Leu Thr Glu Gly Leu Ser Leu Ala Ala Val Asn Gly Pro Asp630563106315Ser Val Val Ile Ala Gly Thr Glu Asp Glu Ala Leu Ala Leu Ala632063256330Glu Ala Phe Ala Ala Glu Gly Arg Lys Thr Gln Arg Leu Pro Val633563406345Ser His Ala Phe His Ser Pro Leu Met Glu Pro Met Leu Asp Ala635063556360Phe Arg Gln Val Ala Glu Ser Leu Glu Tyr Ala Glu Pro Arg Ile636563706375Pro Val Val Ser Asp Val Thr Gly Ala Leu Ala Glu Pro Gly Gln638063856390Leu Thr Arg Ala Ala Tyr Trp Val Glu His Val Arg Ala Thr Val639564006405Arg Phe Ala Asp Gly Val Arg Ala Leu Ala Gly Ala Gly Ala Asn641064156420Ala Phe Leu Glu Ile Gly Pro Gly Gly Val Leu Thr Ala Leu Ala642564306435Gln Ser Val Leu Glu Ala Asp Asp Arg Asp Glu Ala Val Val Leu644064456450Pro Ala Leu Arg Lys Asp Arg Asp Glu Glu Thr Ala Leu Leu Thr645564606465Ala Val Ala Gly Leu His Val Ser Gly Val Arg Val Asp Trp Ser647064756480Ala Trp Phe Thr Gly Thr Gly Ala Arg Arg Thr Glu Leu Pro Thr648564906495
Tyr Ala Phe Gln Arg Glu Arg Tyr Trp Pro Arg Pro Ala Ala Leu650065056510Thr Gly Asp Ile Ser Thr Ala Gly Leu Ile Ser Ala Glu His Pro651565206525Leu Leu Gly Ala Ala Val Pro Leu Ala Asp Ser Glu Gly Val Leu653065356540Phe Thr Ser Gln Ile Ser Met Gln Val His Pro Trp Leu Leu Asp654565506555His Arg Val Gly Gly Thr Val Ile Met Pro Gly Thr Gly Tyr Leu656065656570Glu Met Ala Ile Arg Ala Ala Asp Gln Val Gly Cys Ala Arg Val657565806585Glu Glu Leu Val Leu Ala Ala Pro Met Val Leu Asp Glu Lys His659065956600Pro Val Ser Val Gln Val Val Val Gly Ala Pro Asp Asp Thr Gly660566106615Thr Arg Pro Val Thr Phe Tyr Ser Arg Pro Ser Asp Ala Val Asp662066256630Ala Pro Trp Ser Arg His Ala Asp Gly Phe Leu Ala Val Gln Glu663566406645Arg Thr Asp Ser Phe Glu Ala Pro Val Trp Pro Pro Ala Asp Ala665066556660Val Ser Val Glu Phe Asp Gly Asp Tyr Ser Arg Thr Gly Tyr Gly666566706675Pro Ser Phe Gln Gly Leu Arg Gln Val Trp Leu Arg Gly Glu Glu668066856690Ala Phe Val Glu Val Ala Leu Pro Glu Glu Val Ala Gly Asp Ala669567006705Gln Tyr Phe Gly Met His Pro Ala Leu Leu Asp Ala Val Gln His671067156720Ala Asn Gly Tyr Leu Gly Val Gly Ser Glu Glu Asn Pro Leu Leu672567306735Pro Phe Ala Trp Asn Gly Val Ser Leu His Ala Arg Gly Ala Thr674067456750Thr Leu Arg Val Arg Ile Thr Arg Leu Gly Asp Glu Ser Val Arg675567606765Leu Thr Ala Val Asp Ala Glu Gly Val Pro Val Leu Cys Ala Glu677067756780Ser Leu Val Leu Arg Ala Pro Thr Val Pro Ser Ala Pro Val Ala678567906795Thr Gly Gly Gln Glu Pro Val Phe Arg Leu Asp Trp Thr Ala Ala680068056810Pro Ala Val Lys Pro Thr Glu Gly Leu Thr Ala Ala Thr Leu Gly681568206825
Glu Asp Val Phe Gly Val Gly Thr Ala Leu Ala Ser Leu Thr Glu683068356840Leu Thr Gly Pro Asp Ala Asp Pro Ser Ala Ala Pro Asp Phe Val684568506855Leu Val Pro Leu Ala Gly Ala Gly Asp Ala Ala Gly Ala Gly Asp686068656870Pro Asp Ala His Gly Ala Asp Val Pro Ala Ala Val His Ala Leu687568806885Thr Thr Arg Thr Leu Asp Leu Leu Gln Gln Trp Gln Ala Ala Glu689068956900Arg Leu Gly His Ser Arg Leu Val Phe Val Thr Thr Gly Ala Val690569106915Ala Ala His Asp Thr Glu Thr Val Arg Asp Leu Ala Ala Gly Ala692069256930Ala Trp Gly Leu Val Arg Ser Ala Gln Ser Glu Asn Pro Asp Arg693569406945Phe Val Leu Leu Asp Leu Asp Gly Ala Asp Ala Pro Asp Thr Leu695069556960Arg Ala Leu Leu Pro Asp Leu Pro Gly Leu Leu Gly Ser Gly Asp696569706975Ala Gln Phe Ala Val Arg Glu Gly Thr Ala Leu Val Gly Arg Leu698069856990Glu Arg Leu Thr Thr Ala Pro Gly Leu Leu Ala Pro Thr Gly Thr699570007005Pro Trp Arg Leu Asp Thr Thr Gly Lys Gly Ser Leu Asp Asn Leu701070157020Val Leu Ala Pro Cys Pro Glu Val Leu Gln Pro Leu Gly Ala His702570307035Glu Val Arg Ile Asp Val Glu Ala Ala Gly Leu Asn Phe Arg Asp704070457050Val Leu Asn Ala Leu Gly Met Tyr Pro Gly Glu Ser Gly Pro Met705570607065Gly Thr Glu Ala Ala Gly Val Val Thr Ala Val Gly Glu Ala Val707070757080Thr Gly Leu Ala Pro Gly Asp Arg Val Leu Gly Thr Val Pro Gly708570907095Gly Phe Gly Pro Val Val Val Ala Asp Gln His Tyr Val Thr Gln710071057110Val Pro Glu Gly Trp Thr Met Arg Asp Ala Ala Ser Val Pro Leu711571207125Val Phe Leu Thr Ala Leu Tyr Ala Phe Arg Asp Leu Ala Ser Val713071357140Gln Ala Gly Glu Arg Val Leu Ile His Ala Gly Ala Gly Gly Val714571507155
Gly Met Ala Ala Ile Gln Leu Ala Arg His Val Gly Ala Glu Val716071657170Phe Ala Thr Ala Ser Glu Pro Lys Trp Glu Thr Leu Arg Ser Leu717571807185Gly Leu Asp Asp Ala His Ile Ala Ser Ser Arg Asp Leu Gly Phe719071957200Glu Glu Lys Phe Arg Glu Val Thr Gly Gly Ala Gly Met Asp Val720572107215Val Leu Asn Ala Leu Ala Gly Asp Phe Val Asp Ala Ser Leu Arg722072257230Ile Thr Ala Pro Gly Gly Arg Phe Leu Glu Met Gly Lys Thr Asp723572407245Ile Arg Asp Pro Gln Thr Thr Gly Glu Val Arg Tyr Gln Ala Phe725072557260Asp Leu Gly Glu Ala Gly Pro Glu Arg Asn Lys Ala Leu Leu Gly726572707275Glu Leu Leu Asp Leu Phe Ala Glu Gly Ala Leu Arg Pro Leu Pro728072857290Val Arg Thr Trp Asp Val Arg Arg Ala Arg Glu Ala Phe Arg Phe729573007305Met Ser Gln Ala Lys His Thr Gly Lys Ile Val Leu Thr Met Pro731073157320Pro Arg Trp Asn Pro Glu Gly Thr Val Leu Ile Thr Gly Gly Thr732573307335Gly Ala Leu Gly Gly His Leu Ala Arg Arg Phe Ala Arg Ala Gly734073457350Met Arg His Leu Leu Leu Thr Ser Arg Arg Gly Ala Asp Ala Pro735573607365Gly Ala Ala Glu Leu Ala Ala Glu Leu Arg Glu Leu Gly Ala Glu737073757380Val Thr Val Ala Ala Cys Asp Thr Ala Asp Arg Glu Ala Thr Ala738573907395Ala Leu Leu Asp Ala Val Pro Ala Ala His Pro Leu Thr Ala Val740074057410Val His Thr Ala Gly Ile Leu Asp Asp Gly Val Ile Ala Ser Leu741574207425Thr Pro Glu Arg Leu Ala Ala Val Leu Arg Pro Lys Val Asp Ala743074357440Ala Trp His Leu His Glu Leu Thr Arg Asp Leu Asp Leu Ala Ala744574507455Phe Leu Pro Phe Ser Ser Val Ala Gly Val Met Gly Ser Pro Gly746074657470Gln Gly Asn Tyr Ala Ala Ala Asn Ser Phe Leu Asp Ala Leu Thr747574807485
Arg His Arg Arg His Leu Gly Leu Ala Gly Thr Ser Leu Ala Trp749074957500Gly Pro Trp Ala His Asp Gly Gly Met Thr Ser Thr Leu Ser Asp750575107515Thr Asp Met Arg Arg Met Gln Ser Gly Gly Leu Pro Pro Leu Ala752075257530Val Asp Gln Gly Leu Glu Leu Phe Asp Ile Ala Arg Gly Ser Asp753575407545Glu Ser Phe Leu Val Leu Val Gly Leu Ala Ala Gly Ala Met Arg755075557560Gly Ala Ala Pro Glu Asp Leu Pro Pro Leu Phe Arg Ser Met Val756575707575Arg Ser Gly Arg Arg Thr Ala Ala Ser Thr Asp Ala Ala Gly Ala758075857590Ser Ala Ala Leu Gly Ala Lys Leu Ala Gly Leu Gly Ala Ala Glu759576007605Arg Val Arg Tyr Val Thr Asp Leu Val Arg Glu Gln Ala Ala Arg761076157620Val Leu Gly His Ala Ser Pro Lys Ser Val Asp Val Ser Gln Glu762576307635Phe Arg Glu Leu Gly Val Asp Ser Leu Thr Ala Leu Glu Leu Arg764076457650Asn His Leu Ala Thr Ala Thr Gly Leu Arg Leu Pro Ala Thr Leu765576607665Val Phe Asp Tyr Pro Thr Pro Thr Ala Leu Ala Glu His Phe Val767076757680Ala Glu Leu Ala Gly Asp Asp Ala Val Pro Gln Gly Pro Ser Leu768576907695Leu Ala Glu Leu Asp Arg Phe Glu Thr Leu Leu Ser Ala Gly Asp770077057710Pro Asp Glu Ile Thr Arg Ala Gly Leu Ala Leu Arg Leu Gly Gln771577207725Met Leu Asp Gln Val Arg Gly Asn Ala Pro Glu Gln Ala Gly Ser773077357740Ser Leu Asp Asp Asp Phe Glu Ser Ala Ser Thr Asp Glu Val Phe774577507755Ala Phe Ile Asp Asn Glu Leu Gly Arg Leu Gly Asp Leu776077657770<210>7<211>9550<212>PRT<213>鏈霉菌FR-008
<400>7Met Gly Cys Val Asp Met Asp Lys Glu Gln Lys Leu Arg Asp Tyr Leul 5 10 15Lys Arg Ala Ser Ala Asp Leu Lys Arg Ser Arg Gln Arg Val Thr Glu20 25 30Leu Glu Ala Ala Ala Thr Glu Pro Ile Ala Ile Val Gly Met Ser Cys35 40 45Arg Tyr Pro Gly Gly Val Ser Ser Pro Glu Asp Leu Trp Lys Met Leu50 55 60Val Ala Gly Glu Asp Gly lle Thr Gly Met Pro Glu Asp Arg Gly Trp65 70 75 80Glu Ala Ala Leu Gly Glu Gly Pro Ala Asp Phe Ala Gly Gly Phe Leu85 90 95His Asp Ala Pro Ala Phe Asp Pro Asp Phe Phe Gly Ile Ser Pro Arg100 105 110Glu Ala Leu Ser Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Gly115 120 125Trp Glu Ala Phe Glu Arg Ala Gly Ile Asp Pro Ala Gly Val Arg Gly130 135 140Ser Arg Thr Gly Met Phe Val Gly Ala Met Pro Gln Asp Tyr Arg Val145 150 155 160Gly Pro Asp Asp Asp Val Gln Gly Phe Gln Leu Thr Gly Asn Ala Thr165 170 175Ser Ile Leu Ser Gly Arg Leu Ser Tyr Phe Phe Gly Ala Val Gly Pro180 185 190Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu His195 200 205Leu Ala Ala Gln Ser Leu Arg Ala Gly Glu Cys Ser Leu Ala Leu Ala210 215 220Ala Gly Val Thr Val Met Ala Ser Pro Thr Thr Phe Val Glu Phe Ala225 230 235 240Arg Gln Gly Gly Leu Ala Ala Asp Gly Arg Cys Arg Ser Phe Ala Asp245 250 255Ala Ala Asn Gly Thr Gly Trp Ser Glu Gly Val Gly Val Leu Val Leu260 265 270Glu Arg Leu Ser Glu Ala Arg Arg Asn Gly His Arg Val Leu Ala Val275 280 285Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr290 295 300Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Glu Ser Ala Leu Val305 310 315 320Asn Ala Arg Leu Thr Ala Ala Asp Val Asp Ala Val Glu Ala His Gly325 330 335
Thr Gly Thr Val Leu Gly Asp Pro Val Glu Ala Gln Ala Leu Leu Ala340 345 350Thr Tyr Gly Gln Gly Arg Glu Ala Asp Ser Pro Leu Leu Leu Gly Ser355 360 365Val Lys Ser Asn Ile Ser His Thr Gln Ala Ala Ala Gly Val Ala Gly370 375 380Val Ile Lys Met Val Met Ala Met Arg His Gly Leu Leu Pro Arg Thr385 390 395 400Leu His Val Asp Gln Pro Ser Thr His Val Asp Trp Ser Glu Gly Ser405 410 415eu Leu Thr Glu Pro Ala Pro Trp Pro Ala Arg Glu Asn Ala420 425 430Pro Arg Arg Ala Gly Ile Ser Ser Phe Gly Leu Ser Gly Thr Asn Ala435 440 445His Thr Ile Ile Glu Glu Ala Pro Gln Glu Pro Ala Ala Asp Gly Glu450 455 460Ala Ala Pro Ala Val Asp Ala Gly Ala Leu Pro Trp Leu Leu Ser Gly465 470 475 480Arg Thr Pro Glu Ala Leu Arg Ala Gln Ala Ala Arg Leu Leu Asp His485 490 495Leu Ala Ala Arg Pro Thr Pro Ser Ala Leu Asp Val Ser His Ala Leu500 505 510Ala Thr Thr Arg Ser Ala Leu Glu His Arg Ala Ala Phe Thr Thr Ala515 520 525Asp Arg Asp Thr Ala Val Ala Val Leu Thr Ala Leu Ala Ala Gly Ala530 535 540Asp Ala Pro Gly Leu Thr Thr His Gln Leu Arg Gly Arg Thr Lys Leu545 550 555 560Ala Val Leu Phe Ser Gly Gln Gly Ser Gln Arg Pro Gly Met Gly Arg565 570 575Asp Leu His Ala Arg Phe Pro Val Phe Ala Arg Ala Leu Asp Glu Ile580 585 590Leu Glu His Leu Asp Ala Gly Leu Asp Arg Pro Leu Lys Pro Leu Ile595 600 605Leu Ala Glu Glu Gly Ser Glu Glu Ala Ala Leu Leu Asp Arg Thr Glu610 615 620Tyr Ala Gln Pro Ala Leu Phe Ala Leu Glu Val Ala Leu Tyr Arg Leu625 630 635 640Val Glu Ser Trp Gly Val Thr Pro Asp His Leu Thr Gly His Ser Val645 650 655Gly Glu Ile Ala Ala Ala His Val Ala Gly Val Phe Thr Leu Ala Asp660 665 670Ala Cys Thr Leu Val Thr Ala Arg Gly Arg Leu Met Gln Ala Leu Asp675 680 685
Glu Gly Gly Ala Met Val Ser Leu Glu Ala Thr Glu Asp Glu Val Ala690 695 700Pro Leu Val Ala Glu His Ala Gly Gln Val Ser Val Ala Ala Val Asn705 710 715 720Gly Pro Ala Ala Val Val Val Ser Gly Ala Glu Asp Ala Val Glu Ser725 730 735Ile Ala Ala Arg Val Ala Ala Leu Gly Arg Arg Thr Arg Arg Leu Arg740 745 750Val Ser His Ala Phe His Ser Pro Leu Met Asp Pro Met Leu Glu Glu755 760 765Phe Arg Ala Val Val Ala Gly Leu Ser Pro Gln Ala Pro Thr Val Pro770 775 780Val Val Ser Asn Leu Thr Gly Ala Pro Ala Thr Val Glu Gln Leu Thr785 790 795 800Ser Ala Gly Tyr Trp Thr Asp His Val Arg His Ala Val Arg Phe Ala805 810 815Asp Gly Val Ser Trp Leu Ala Gly His Gly Thr Gly Leu Phe Leu Glu820 825 830Leu Gly Pro Asp Ala Thr Leu Ala Ala Leu Thr Arg Thr Val Leu Asp835 840 845Ala Ala Gly His Glu Arg Ala Ala Val Leu Pro Ala Leu Arg Lys Asp850 855 860Arg Pro Glu Ala Ala Thr Leu Thr Glu Thr Ala Thr Gly Leu His Leu865 870 875 880Arg Gly Val Pro Val Arg Trp Asp Arg Trp Phe Glu Gly Thr Gly Ala885 890 895Gly His Thr Asp Leu Pro Thr Tyr Ala Phe Gln His Arg Arg Phe Trp900 905 910Pro Lys Gly Val Thr Gly Leu Thr Gly Asp Met Arg Ser Ala Gly Leu915 920 925Gly Ala Ala His His Pro Leu Leu Ala Ala Ala Val Thr Leu Ala Asn930 935 940Ser Asp Gly Leu Leu Leu Thr Gly Arg Leu Ser Val Arg Thr His Pro945 950 955 960Trp Leu Ala Asp His Thr Val Arg Gly Thr Val Leu Leu Ala Gly Thr965 970 975Ala Phe Leu Glu Leu Ala Val Arg Ala Gly Asp Glu Val Gly Cys Asp980 985 990Arg Val Glu Glu Leu Thr Leu Ala Ala Pro Leu Ala Leu Pro Glu Asp995 10001005Gly Gly Val Gln Val Gln Val Trp Ile Gly Ser Pro Asp Glu Thr101010151020Gly Arg Arg Ala Leu Thr Leu Tyr Ser Arg Pro Asp Gly Asp Asp102510301035
Asp Arg Pro Trp Thr Gln His Ala Ala Gly Thr Leu Thr Asn Gly104010451050Ala His His Gly Glu Glu Phe Asp Ala Ala Val Trp Pro Pro Ala105510601065Gly Ala Glu Ser Val Gly Leu Asp Gly Phe Tyr Glu Gly Leu Ala107010751080Glu Ser Gly Phe Ala Tyr Gly Pro Ala Phe Arg Gly Leu Arg Ala108510901095Val Trp Arg Arg Gly Glu Glu Thr Phe Ala Glu Val Ala Leu Asp110011051110Ala Gly Pro Ala Gly Asp Ala Ser Ser Phe Gly Leu His Pro Ala111511201125Leu Leu Asp Ala Ala Leu His Ala Thr Ala Leu Ala Pro Leu Gly113011351140Glu Asp Ala Arg Gly Gly Leu Pro Phe Ala Trp Gln Asp Val Thr114511501155Leu His Ala Ala Gly Ala Thr Glu Ala Arg Val Arg Ile Thr Pro116011651170Ala Gly Asp Asp Ala Val Thr Leu Thr Val Ala Asp Thr Thr Gly117511801185Ala Pro Val Ala Ser Val Gly Ser Leu Val Leu Arg Ser Ala Pro119011951200Asp Ala Arg Arg Ala Ala Ala Gly Val Leu Glu Arg Asp Ala Leu120512101215Phe His Leu Asp Trp Thr Arg Leu His Gly Ala Asp Ala Pro Glu122012251230Thr Pro Gly Thr Val Gly Leu Leu Gly Pro Asp Pro Leu Arg Leu123512401245Gly Ala Ala Leu Thr Ala Ala Gly Val Thr Val Gly Pro Leu Thr125012551260Glu Ala Ala Gly Pro Asp Thr Pro Ala Leu Val Leu Val Pro Val126512701275Gly Gly Gly Asp Pro Glu Ala Val Pro Ala Glu Val His Ala Ser128012851290Thr Ala Arg Val Leu Gly His Ile Gln Arg Trp Leu Ala Glu Asp129513001305Thr Arg Ala Gly Ala Arg Leu Val Phe Val Thr Arg Gly Ala Val131013151320Ala Val Gly Glu Gly Pro Leu Thr Asp Pro Ala Ala Ala Ala Val132513301335Trp Gly Leu Val Arg Ser Ala Gln Ala Glu His Pro His Arg Phe134013451350Gly Leu Leu Asp Leu Asp Pro Val Ala Gly Thr Asp Pro Ala Ala135513601365
Leu Val Ala Ala Leu Ala Ala Glu Glu Pro Gln Thr Ala Leu Arg137013751380Gly Asp Thr Val Phe Ala Ala Arg Leu Ala Arg Val Arg Pro Asp138513901395Gly Glu Thr Thr Ala Ala Ala Trp Asn Pro Glu Gly Thr Val Val140014051410Val Thr Gly Gly Thr Gly Gly Leu Gly Ala Ala Leu Ala Arg His141514201425Leu Val Thr Glu Arg Gly Val Arg His Leu Val Leu Ala Gly Arg143014351440Arg Gly Pro Asp Ala Pro Gly Ala Ala Gly Leu Ala Ala Asp Leu144514501455Gly Ala Leu Gly Ala Glu Val Thr Leu Ala Ala Cys Asp Val Ala146014651470Asp Pro Glu Ala Val Asp Arg Leu Phe Ala Ala Val Pro Ala Ala147514801485His Pro Val Thr Ala Val Val His Thr Ala Gly Thr Leu Asp Asp149014951500Gly Leu Val Glu Ser Leu Thr Glu Glu Arg Leu Ala Ala Val Leu150515101515Arg Pro Lys Ala Asp Ala Val Trp His Leu His Arg Ala Thr Arg152015251530Asp Leu Asp Leu Ala Ala Phe Val Val Phe Ser Ser Leu Ala Gly153515401545Thr Thr Gly Ala Pro Gly Gln Ala Asn Tyr Ala Ala Gly Asn Ala155015551560Phe Leu Asp Ala Val Ala Arg Leu Arg Arg Asp Ala Gly Leu Pro156515701575Gly Leu Ser Leu Gly Trp Gly Pro Trp Val Pro Val Asp Gly Gly158015851590Met Thr Gly Glu Leu Ser Glu Arg Asp Leu Glu Arg Met Ser Arg159516001605Ser Gly Thr Pro Pro Leu Thr Val Glu Gln Gly Leu Ala Leu Phe161016151620Asp Ala Thr Leu Ala Leu Pro Arg Ala Ala Val Leu Pro Val Arg162516301635Leu Asp Leu Ala Ala Leu Arg Gly Arg Gly Glu Val Pro Pro Leu164016451650Leu Arg Gly Leu Val Arg Thr Arg Ala Arg Arg Thr Val Arg Thr165516601665Gly Ser Glu Ala Ala Leu Gly Leu Ala Gln Arg Leu Ala Arg Leu167016751680Asp Glu Gln Ala Arg Thr Glu Ala Val Val Asp Leu Val Arg Asp168516901695
Arg Thr Ala Ala Val Leu Gly His Ala Ser Ala Ala Asp Ile Asp170017051710Pro Glu Arg Pro Phe Gln Glu Leu Gly Phe Asp Ser Leu Thr Ala171517201725Val Glu Leu Arg Asn Gly Leu Ala Ala Ala Thr Gly Leu Arg Thr173017351740Ser Ala Thr Val Ile Phe Asp Tyr Pro Thr Val Ala Ala Leu Ala174517501755Gly His Leu Leu Ala Glu Leu Thr Asp Ala Pro Glu Ala Glu Ala176017651770Pro Ala Pro Ala Pro Arg Thr Ser Ala Thr Ala Asp Asp Pro Ile177517801785Val Ile Val Gly Met Ser Cys Arg Tyr Pro Gly Gly Val Gly Ser179017951800Pro Asp Asp Leu Trp Arg Leu Val Thr Glu Gly Thr Asp Ala Ile180518101815Gly Gly Leu Pro Val Asn Arg Gly Trp Asp Leu Asp Ser Leu Tyr182018251830His Pro Asp Pro Asp His Pro Gly Thr Ser Tyr Thr Arg Tyr Gly183518401845Gly Phe Leu His Asp Ala Gly Glu Phe Asp Ala Gly Phe Phe Gly185018551860Met Ser Pro Arg Glu Ala Leu Ala Thr Asp Ser Gln Gln Arg Leu186518701875Leu Leu Glu Ala Ser Trp Thr Ala Val Glu His Ala Gly Ile Asp188018851890Pro Val Gly Leu Arg Gly Ser Arg Thr Gly Val Phe Ala Gly Val189519001905Met Tyr Ser Asp Tyr Ser Ala Thr Leu Ala Asp Glu Arg Phe Glu191019151920Gly His Gln Gly Ser Gly Thr Ala Pro Ser Ile Ala Ser Gly Arg192519301935Val Ser Tyr Ala Leu Gly Leu Glu Gly Pro Ala Val Thr Val Asp194019451950Thr Ala Cys Ser Ser Ser Leu Val Ala Met His Trp Ala Met Gln195519601965Ala Leu Arg Ser Gly Glu Cys Ser Leu Ala Leu Ala Gly Gly Val197019751980Thr Val Met Ser Thr Pro Thr Ser Leu Ile Glu Phe Ser Arg Gln198519901995Arg Gly Leu Ser Pro Asp Gly Arg Cys Lys Ala Phe Ser Asp Glu200020052010Ala Asp Gly Val Gly Trp Ser Glu Gly Val Gly Met Leu Val Leu201520202025
Glu Arg Leu Ser Asp Ala Arg Arg Asn Gly His Arg Val Leu Ala203020352040Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly204520502055Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gln206020652070Ala Leu Asp Thr Ala Gly Leu Thr Thr Ala Asp Val Asp Ala Val207520802085Glu Ala His Gly Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala209020952100Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asp Arg Pro Gly Asp Arg210521102115Pro Leu Leu Leu Gly Ser Val Lys Ser Asn Ile Gly His Thr Gln212021252130Ala Ala Ala Gly Val Ala Gly Ile Ile Lys Met Val Met Ala Met213521402145Arg His Gly Glu Leu Pro Arg Thr Leu His Ala Gly Ser Pro Ser215021552160Arg His Val Asp Trp Glu Thr Gly Asp Val Arg Ile Leu Gln Glu216521702175Pro Glu Ala Trp Pro Glu Thr Gly Arg Pro Arg Arg Ala Gly Val218021852190Ser Ser Phe Gly Ile Ser Gly Thr Asn Ala His Val Val Leu Glu219522002205Gln Gly Glu Ser Ala Pro Ala Gly Ser Thr Gly Thr Pro Pro Ala221022152220Glu Ala Thr Val Leu Pro Trp Thr Leu Ser Gly Arg Thr Arg Pro222522302235Ala Leu Arg Ala Gln Ala Ala Arg Leu Leu Ala His Leu Asp Ala224022452250Arg Pro Asp Glu Ala Pro Ala Asp Leu Thr His Ser Leu Ala Thr225522602265Thr Arg Pro Ala Phe Glu Gln Arg Ala Val Val Val Gly Thr Ala227022752280Asp Asp Ala Arg Arg Ala Leu Ala Ala Leu Ala Ala Asp Arg Pro228522902295Asp Pro Ala Leu Val Leu Gly Glu Thr Gly Gly Thr Gly Arg Thr230023052310Ala Leu Leu Phe Thr Gly Gln Gly Ser Gln Arg Pro Gly Thr Gly231523202325Arg Glu Leu Tyr Glu Arg His Pro Val Phe Ala Glu Ala Leu Asp233023352340Glu Val Val Ala Arg Leu Asp Pro Leu Leu Val Asp Ala Pro Ala234523502355
Gly Thr Pro His Gly Ser Leu Lys Asp Val Met Phe Ala Glu Glu236023652370Gly Thr Pro Gly Ala Ala Ala Leu Tyr Glu Thr Gly Trp Thr Gln237523802385Pro Ala Leu Phe Ala Leu Glu Thr Ala Leu Tyr Arg Leu Val Arg239023952400Ser Trp Gly Val Arg Pro Gly Val Leu Leu Gly His Ser Val Gly240524102415Gly Ile Gly Ala Ala His Ala Ala Gly Val Leu Ser Leu Asp Asp242024252430Ala Cys Ala Leu Val Ala Ala Arg Ala Arg Leu Met Gln Ala Leu243524402445Pro Glu Gly Gly Ala Met Trp Ser Leu Gln Ala Ser Glu Asp Glu245024552460Val Leu Pro Leu Leu Gln Gly Gln Glu Asp Val Ala Ala Leu Ala246524702475Ala Val Asn Gly Pro Ser Ala Val Val Val Ser Gly Glu Glu Glu248024852490Ala Thr Ala Ala Ile Ala Ala His Phe Ala Gly Leu Gly Arg Ala249525002505Thr Arg Arg Leu Arg Val Ser His Ala Phe His Ser Pro Arg Met251025152520Asp Ala Val Leu Asp Ala Phe Arg Glu Val Ala Glu Gly Leu Thr252525302535Tyr His Glu Pro Thr Val Pro Val Ala Cys Asp Leu Thr Gly Arg254025452550Leu Ala Glu Gly Asp His Leu Arg Thr Ala Asp Tyr Trp Val Arg255525602565His Val Arg Ser Thr Val Arg Phe Ala Asp Ala Val Arg Ala Ala257025752580His Glu Ala Gly Ala Thr Thr Tyr Leu Glu Leu Gly Pro Gly Gly258525902595Ser Leu Cys Ala Ala Ala Gln Asp Thr Leu Gly Asp Asp Ser Glu260026052610Ala Asp Ala Val Pro Thr Leu Arg Thr Gly Arg Pro Glu Asp Glu261526202625Ser Val Leu Thr Ala Leu Ala Arg Leu His Val Arg Gly Val Arg263026352640Val Asp Trp Gly Ala Val His Pro Ala Gly Ala Gly Thr Val Glu264526502655Leu Pro Thr Tyr Ala Phe Gln His Glu Thr Tyr Trp Pro Asp Thr266026652670Thr Ala Pro Thr Val Arg Arg Thr Gly Asp Gly Ser Gly Asp Pro267526802685
Ala Asp Ala Glu Leu Trp Thr Ala Val Glu Arg Gly Asp Ala Thr269026952700Gly Leu Ala Gly Leu Leu Gly Leu Arg Asp Glu Glu His Ala Ser270527102715Leu Tyr Thr Leu Leu Pro Ser Leu Ser Ser Trp Arg Arg Ala Arg272027252730Gln Glu Arg Ala Val Leu Asp Ala Ala Arg Tyr Arg Ile Ala Trp273527402745Gln Pro Ala Gly Thr Glu Ser Ala Pro Val Leu Asp Gly Thr Trp275027552760Leu Ala Val Thr Thr Glu Asp Asp Gly Ala Ala Asp Thr Val Leu276527702775Ala Ala Leu Arg Gly His Gly Ala Val Val Glu Arg Leu Val Leu278027852790Asp Ala Ser His Leu Asp Arg Asp Leu Leu Thr Thr Thr Leu Arg279528002805Asp Ala Val Glu Asn Ala Thr Ala Glu Gly Ala Ala Gly Val Arg281028152820Gly Ile Leu Ser Leu Leu Pro Leu Ala Asp Ala Ala Arg Pro Glu282528302835Ala Thr Gly Thr Asp Ala Pro Ala Gly Gly Leu Pro Ala Gly Phe284028452850Ala Leu Gly Val Val Leu Ala Gln Ala Leu Gly Asp Ala Ala Val285528602865Thr Ala Pro Leu Trp Thr Val Thr Arg Gly Ala Val Ser Thr Gly287028752880Pro Gly Asp Pro Leu Thr His Pro Ala Arg Ala Ala Ala Trp Gly288528902895Leu Gly Arg Val Ala Ala Leu Glu Arg Pro Glu Gln Trp Ser Gly290029052910Leu Val Asp Leu Pro Glu Val Leu Asp Ala Pro Ala Thr Gln Arg291529202925Leu Val Ser Leu Leu Ala Thr Arg Asp Gly Glu Asp Gln Leu Ala293029352940Val Arg Ala Gly Gly Thr Leu Ala Arg Arg Val Val Arg His Pro294529502955Gly Asp Ala Leu Pro Arg Glu Asp Glu Phe Thr Ala Ser Gly Thr296029652970Val Leu Val Thr Gly Gly Thr Gly Gly Leu Gly Ala Glu Ala Ala297529802985Arg Trp Leu Ala Arg Ser Gly Ala Ala His Leu Val Leu Thr Gly299029953000Arg Arg Gly Pro Asp Ala Pro Gly Ala Ala Glu Leu Arg Ala Glu300530103015
Leu Glu Glu Leu Gly Ala Arg Val Thr Leu Thr Ala Cys Asp Ser302030253030Ala Asp Arg Asp Ala Leu Ala Ala Val Leu Ala Ala Val Pro Glu303530403045Asp Ala Pro Leu Thr Gly Val Val His Ala Ala Gly Val Gly Gln305030553060Ala Ala Pro Leu Ala Asp Thr Pro Leu Asp Gln Val Ala Ala Ala306530703075Met Ala Ala Lys Thr Leu Gly Ala Ala His Leu Asp Ala Leu Leu308030853090Asp Gly His Asp Leu Asp Phe Leu Val Leu Val Ser Ser Val Ala309531003105Gly Val Trp Gly Ser Ala Gly Gln Ser Ala Tyr Ala Ala Ala Asn311031153120Ala Tyr Leu Asp Ala Leu Ala Glu Asn Arg Arg Ser Arg Gly Leu312531303135Ala Ala Thr Ser Val Ala Trp Gly Pro Trp Ala Glu Val Gly Met314031453150Ala Ser Ala His Arg Thr Val Ser Glu Asn Leu Glu Arg Ala Gly315531603165Leu Arg Leu Leu Asn Pro Ala Thr Ala Met Thr Glu Leu Arg Arg317031753180Ala Val Val Arg Gly Glu Thr Thr Val Thr Val Ala Asp Val Asp318531903195Trp Glu Gln Tyr His Pro Val Phe Thr Ala Ala Arg Ser Ser Arg320032053210Leu Phe Asp Thr Val Asp Thr Val Ala Ala Leu Ala Ala Pro Gly321532203225Ser Thr Gly Thr Val Ser Gly Leu Ala Glu Arg Leu Gly Gly Leu323032353240Thr Glu Gln Glu Gln Glu Arg Leu Leu Val Asp Leu Val Arg Ala324532503255Glu Ala Ala Val Val Leu Gly His Thr Ser Ala Glu Gly Val Pro326032653270Val Lys Lys Ala Phe Arg Asp Ala Gly Phe Asp Ser Leu Thr Ala327532803285Val Glu Leu Arg Lys Arg Leu Ala His Leu Thr Gly Leu Ala Leu329032953300Pro Ser Thr Leu Val Phe Asp Tyr Pro Asn Pro Leu Ala Leu Ala330533103315Arg His Leu Arg Ala Glu Leu Leu Gly Thr Val Glu Thr Ala Pro332033253330Val Ala Thr Gly Pro Ala Val Thr Asp Glu Pro Ile Ala Ile Ile333533403345
Gly Met Ser Cys Arg Phe Pro Gly Gly Ala Arg Ser Ala Asp Ala335033553360Phe Trp Gln Leu Val Ala Asp Asn Thr Asp Ala Ile Ser Glu Phe336533703375Pro Val Asn Arg Gly Trp Asp Gly Asp Ala Leu Tyr Asp Pro Asp338033853390Pro Asp Arg Pro Gly Thr Thr Tyr Ser Thr Gln Gly Gly Phe Leu339534003405His Asp Ala Gly Glu Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro341034153420Arg Glu Ala Val Ser Met Asp Pro Gln Gln Arg Leu Leu Leu Glu342534303435Thr Thr Trp Glu Ala Phe Glu His Ala Gly Ile Asp Pro Ala Ala344034453450Val His Ser Thr Pro Thr Gly Thr Phe Ile Gly Ser Thr Tyr Gln345534603465Asp Tyr Gly Val Ser Met Asp Asp Gly Ser Ala Gly His Ala Val347034753480Thr Gly Ser Ser Pro Ser Val Leu Ser Gly Arg Leu Ala Tyr Ser348534903495Phe Gly Leu Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser350035053510Ser Ser Leu Val Ala Leu His Leu Ala Cys Gln Ser Leu Arg Asn351535203525Gly Glu Thr Thr Leu Ala Leu Ala Gly Gly Ala Thr Val Met Thr353035353540Asn Pro Met Pro Phe Ile Ala Phe Ser Arg Gln Arg Ala Leu Ala354535503555Arg Asp Gly Arg Cys Lys Ala Phe Ser Glu Ser Ala Asp Gly Met356035653570Thr Leu Ala Glu Gly Val Gly Ile Leu Val Leu Glu Arg Leu Ser357535803585Asp Ala Arg Arg Asn Gly His Glu Val Leu Ala Val Val Arg Gly359035953600Ser Ala Ile Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro360536103615Asn Gly Pro Ser Gln Val Arg Val Ile Arg Gln Ala Leu Ala Ala362036253630Ser Gly Leu Thr Thr Asp Asp Ile Asp Ala Val Glu Ala His Gly363536403645Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu His365036553660Ala Thr Tyr Gly Arg Asp Arg Asp Pro Glu Asn Pro Leu Leu Leu366536703675
Gly Ser Val Lys Ser Asn Ile Gly His Thr Gln Ser Ala Ala Gly368036853690Val Ala Gly Ile Ile Lys Met Val Met Ala Met Arg His Gly Thr369537003705Leu Pro Arg Thr Leu His Ala Thr Glu Pro Ser Ser His Ile Asp371037153720Trp Ser Gly Gly Thr Val Arg Leu Val Thr Glu Asn Thr Gly Trp372537303735Ala Arg Asp Asp Arg Pro Leu Arg Ala Ala Val Ser Ser Phe Gly374037453750Ile Ser Gly Thr Asn Ala His Ala Ile Leu Glu Gln Ala Ala Pro375537603765Ala Glu Glu Pro Ala Pro Ala Ala Gly Pro Ala Leu Pro Thr Gly377037753780Gly Thr Leu Pro Trp Ile Leu Ser Ala Ala Ala Pro Ser Ala Leu378537903795Arg Glu Gln Ala Ala Asn Leu Ala Ala His Val Thr Ser Gly Ala380038053810Ala Pro His Pro Ala Asp Val Gly His Thr Leu Ile Thr Ala Arg381538203825Ser Val Leu Glu His Arg Ala Val Ala Leu Gly Ala Asp Ala Gly383038353840Glu Leu Thr Asp Ala Leu Ala Ala Phe Gly Ala Gly Glu Ser Thr384538503855Gly Gln Val Val His Gly Thr Ala Asp Thr Asp Gly Arg Thr Val386038653870Phe Val Phe Pro Gly Gln Gly Ser Gln Trp Ala Gly Met Gly Ala387538803885Glu Leu Leu Asp Thr Ser Pro Val Phe Ala Glu Arg Leu His Ala389038953900Cys Ala Ala Ala Leu Ala Pro Tyr Thr Asp Trp Asp Leu Val Asp390539103915Val Val Arg Gln Ala Asp Gly Ala Pro Thr Leu Asp Arg Val Asp392039253930Val Val Gln Pro Ala Thr Trp Ala Val Met Val Ser Leu Ala Glu393539403945Leu Trp Arg Ala His Gly Val Thr Pro Asp Ala Val Ile Gly His395039553960Ser Gln Gly Glu Ile Ala Ala Ala Val Val Ser Gly Ala Leu Thr396539703975Leu Asp Asp Gly Ala Arg Val Val Ala Leu Arg Ser Gln Ala Ile398039853990Arg Arg Val Leu Ala Gly Ala Gly Gly Met Met Ser Val Gln Val399540004005
Ser Ala Ala Glu Ala Glu Gln Tyr Leu Thr Pro His Ala Gly Ala401040154020Val Ser Val Ala Ala Val Asn Gly Pro Arg Ser Val Val Leu Ala402540304035Gly Thr Pro Glu Ala Leu Asp Ala Leu Gln Ala Glu Phe Ala Ala404040454050Arg Asp Leu Arg Ala Arg Arg Ile Ala Val Asp Tyr Ala Ser His405540604065Thr Ala Gln Val Glu Arg Ile Glu Ala Glu Leu Leu Asp Leu Leu407040754080Ala Pro Val Ala Pro Arg Ala Ala Arg Val Pro Phe His Ser Thr408540904095Val Thr Gly Ala Thr Leu Asp Thr Thr Gly Met Asp Ala Ala Tyr410041054110Trp Tyr Arg Asn Leu Arg Gln Thr Val Arg Phe Glu Asp Gly Val411541204125Arg Ala Leu Leu Asp Thr Glu His Thr Leu Phe Val Glu Ile Ser413041354140Pro His Pro Val Leu Thr Met Ala Val Gln Ala Thr Ala Glu Glu414541504155Ala Gly Arg Pro Ala Ala Ala Val Gly Thr Leu Arg Arg Glu Gln416041654170Gly Gly Thr Ala Arg Phe Leu Thr Ser Leu Ala Glu Gln Trp Val417541804185Arg Gly Gly Arg Ala Asp Trp Asn Ala Val Tyr Ala Asp Thr Gly419041954200Ala Arg Arg Thr Pro Leu Pro Thr Tyr Ala Phe Gln Arg Glu His420542104215Leu Trp Ala Val Ser Ala Arg Pro Gln Gly Ser Gly Asp Gly Asp422042254230Pro Ala Asp Ala Glu Phe Trp Ala Glu Val Glu Gln Glu Asp Ala423542404245Glu Ser Leu Ala Ser Arg Leu Glu Leu Asp Arg Glu Ala Leu Ala425042554260Pro Leu Leu Pro Ala Leu Ser Thr Trp Arg Arg Gly Arg Arg Asp426542704275Arg Ser Thr Val Gly Ser Trp Arg Tyr Arg Ala Thr Trp Lys Pro428042854290Leu Gly Ala Leu Pro Ser Ala Thr Leu Asp Gly Thr Trp Leu Leu429543004305Val Thr Ala Glu Gly Thr Asp Pro Glu Tyr Ala Ala Ala Val Ala431043154320Glu Gln Leu Thr Ala His Gly Ala Arg Thr Val Pro Leu Ala Leu432543304335
Thr Gly Ala Asp Ala Asp Arg Ala Ala Leu Thr Glu Arg Leu Arg434043454350Asp Leu Pro Glu Pro Ala Ala Val Val Ser Leu Leu Ala Asp Asp435543604365Glu Ser Thr Gly Ala Ala His Pro Val Leu Thr Thr Gly Leu Ala437043754380Leu Ser Val Thr Leu Thr Gln Ala Leu Gly Asp Ala Gly Ile Glu438543904395Ala Pro Leu Trp Ala Leu Thr Arg Gly Ala Val Ser Thr Gly Arg440044054410Ala Asp Arg Leu Thr Arg Pro Ala Gln Ala Leu Val Gln Gly Phe441544204425Gly Trp Thr Ala Ala Leu Glu His Pro Asp Arg Trp Gly Gly Thr443044354440Val Asp Leu Pro Glu Thr Leu Asp Arg Arg Ala Gly Glu Arg Leu444544504455Ala Ala Val Leu Ala Gly Thr Thr Gly Glu Asp Gln Leu Ala Val446044654470Arg Ala Ser Gly Val Leu Ala Arg Arg Val Ser Pro Ala Pro Ala447544804485Pro Thr Gly Thr Gly Arg Trp Ser Pro Arg Gly Thr Val Leu Val449044954500Thr Gly Gly Thr Gly Thr Leu Gly Pro His Leu Ala Arg Trp Leu450545104515Ala Asp Gln Gly Ala Arg Asp Leu Val Leu Thr Ser Arg Arg Gly452045254530Ala Asp Ala Pro Gly Ala Ala Glu Leu Ala Ala Glu Leu Gly Glu453545404545Arg Gly Cys Arg Val Thr Val Ala Ala Cys Asp Val Thr Asp Arg455045554560Glu Ala Val Ala Ala Leu Leu Asp Gly Leu Thr Ala Glu Gly Arg456545704575Thr Val Arg Ser Val Phe His Thr Ala Ala Val Ile Glu Leu Gln458045854590Ser Ile Glu Glu Thr Ser Leu Asp Ala Phe Ala Lys Val Val His459546004605Ala Lys Thr Ala Gly Ala Ala His Leu Asp Glu Leu Leu Asp Asp461046154620Asp Gln Leu Asp Ala Phe Val Leu Tyr Ser Ser Thr Ala Gly Met462546304635Trp Gly Ser Gly Arg His Ala Ala Tyr Val Ala Ala Asn Ala His464046454650Val Asn Ala Leu Ala Glu His Arg Cys Ala Arg Gly Ala His Gly465546604665
Thr Ala Val Ser Trp Gly Ile Trp Ala Asp Asp Met Lys Leu Gly467046754680Arg Val Asp Pro Gly Gln Ile Arg Arg Ser Gly Leu Glu Phe Met468546904695Asp Pro Arg Leu Ala Leu Ala Gly Leu Arg Gln Val Leu Asp Gly470047054710Asp Gln Gly Ala Val Ala Val Ala Asp Val Asp Trp Asp Arg Tyr471547204725His Pro Val Phe Thr Ser Ser Arg Pro Thr Glu Leu Phe Glu Asn473047354740Val Pro Glu Val Arg Arg Leu Thr Glu Gln Gln Glu Ala Arg Ala474547504755Gly Glu Ala Gly Glu Phe Ile His Arg Leu Arg Ser Leu Pro Ala476047654770Gly Glu Gln Asp Arg Leu Leu Leu Glu Leu Val Arg Ser Glu Ala477547804785Ala Thr Val Leu Gly His Ala Ser Pro Glu Val Leu Ser Glu Arg479047954800Arg Ala Phe Arg Asp Ile Gly Phe Asp Ser Leu Thr Ala Val Asp480548104815Leu Arg Asn Arg Leu Ala Ser Val Thr Gly Leu Thr Leu Pro Ser482048254830Thr Leu Val Phe Asp Tyr Pro Asp Pro Leu Thr Leu Val Gly His483548404845Leu Arg Gly Leu Ala Gly Gly Pro Ala Ala Gly Pro Asp Gly Pro485048554860Val Arg Thr Thr Ala Ala Thr Asp Asp Glu Pro Ile Ala Val Val486548704875Ala Met Ser Cys Arg Tyr Pro Gly Gly Val Thr Ser Pro Glu Ala488048854890Leu Trp Glu Leu Ile Ala Ala Gly Ala Asp Ala Ile Thr Gly Phe489549004905Pro Ala Asp Arg Gly Trp Asp Ala Asp Ala Leu Tyr Asp Pro Asp491049154920Pro Asp Arg Pro Gly Thr Thr Tyr Ser Thr Gln Gly Gly Phe Leu492549304935His Asp Val Ala Asp Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro494049454950Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu Glu495549604965Thr Gly Trp Glu Ala Ile Glu Arg Ala Gly Ile Asp Pro Ala Thr497049754980Leu Arg Gly Ser Met Thr Gly Thr Phe Ile Gly Ala Ser Tyr Gln498549904995
Asp Tyr Thr Ala Gly Gly Ala Gly Ser Glu Gly Ala Glu Asp His500050055010Leu Ile Thr Gly Thr Ile Ser Ser Val Met Ser Gly Arg Leu Ala501550205025Tyr Thr Phe Gly Leu Glu Gly Pro Ala Val Thr Leu Asp Thr Ala503050355040Cys Ser Ser Ser Leu Val Ala Leu His Leu Ala Cys Gln Ser Leu504550505055Arg Asn Gly Glu Ser Thr Leu Ala Leu Ala Gly Gly Val Ser Val506050655070Met Ala Thr Pro Gln Ala Phe Thr Gly Phe Ser Arg Gln Arg Ala507550805085Met Ala Pro Asp Gly Arg Cys Lys Ala Tyr Ala Glu Gly Ala Asp509050955100Gly Met Ser Leu Ala Glu Gly Val Gly Leu Val Leu Val Glu Arg510551105115Leu Ser Asp Ala Arg Arg Asn Gly His Pro Val Leu Ala Val Ile512051255130Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr513551405145Ala Pro Asn Gly Pro Ser Gln Ala Arg Val Ile Arg Gln Ala Leu515051555160Ala Asn Ala Arg Leu Thr Pro Gly Glu Val Asp Val Val Glu Gly516551705175His Gly Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala518051855190Leu Leu Ala Thr Tyr Gly Gln Asp Arg Pro Gly Asp Arg Pro Leu519552005205Leu Leu Gly Ser Val Lys Ser Asn Ile Gly His Thr Gln Met Ala521052155220Ser Gly Ile Ala Gly Val Met Lys Met Val Met Ala Met Arg His522552305235Gly Thr Val Pro Arg Thr Leu His Val Asp Thr Pro Ser Ser His524052455250Val Asp Trp Gly Ser Gly Ala Ile Asp Leu Ala Thr Glu Thr Val525552605265Pro Trp Pro Glu Thr Gly Arg Pro His Arg Ala Ala Val Ser Ser527052755280Phe Gly Leu Ser Gly Thr Asn Val His Thr Ile Val Glu Gln Ala528552905295Pro Glu Glu Pro Ala Thr Glu Pro Ser Ala Ser Pro Ala Pro Ala530053055310Gly Asp Pro Ala Thr Leu Pro Pro Gly Val Pro Val Val Leu Ser531553205325
Ala Arg Thr Pro Ala Ala Leu Arg Glu Gln Ala Glu Arg Leu Ala533053355340Gly His Leu Ala Gly Arg Pro Asp Val Pro Leu Val Asp Leu Ala534553505355His Ser Leu Ala Thr Thr Arg Gly Val Leu Glu His Arg Ala Ala536053655370Val Leu Thr Gly Asp Arg Asp Gly Leu Leu Arg Ala Leu Thr Ala537553805385Leu Arg Glu Gly Ala Pro Asp Ala Ser Leu Leu Thr Gly Ala Pro539053955400Ala Arg Gly Gly Leu Ala Phe Leu Phe Thr Gly Gln Gly Ser Gln540554105415Arg Pro Ala Met Gly Arg Asp Leu Tyr Glu Arg His Pro Val Phe542054255430Gly Glu Ala Leu Asp Ala Val Leu Thr Arg Phe Asp Gln Glu Leu543554405445Asp Arg Pro Leu Arg Glu Val Leu Phe Ala Glu Glu Gly Thr Ala545054555460Glu Ala Ala Leu Leu Asp Asp Thr Ala Tyr Thr Gln Pro Ala Leu546554705475Phe Ala Leu Glu Val Ala Leu Tyr Arg Leu Val Glu Ser Trp Gly548054855490Val Arg Pro Asp Gln Val Ala Gly His Ser Val Gly Glu Ile Ala549555005505Ala Ala His Val Ala Gly Val Phe Ser Leu Asp Asp Ala Cys Ala551055155520Leu Val Ala Ala Arg Gly Arg Leu Met Ala Ala Leu Pro Ala Gly552555305535Gly Ala Met Val Ala Val Glu Ala Ser Glu Glu Glu Val Leu Pro554055455550Leu Leu Glu Gly Gly Glu Asp Arg Ile Ser Leu Ala Ala Val Asn555555605565Gly Pro Arg Ala Val Val Ile Ala Gly Asp Glu Glu Pro Val Ala557055755580Ala Val Ala Ala Arg Leu Ala Glu Asp Gly Arg Arg Thr Arg Ala558555905595Leu Lys Val Ser His Ala Phe His Ser Pro Arg Met Asp Ala Met560056055610Leu Asp Asp Phe Ala Arg Val Ala Arg Gly Leu Thr Tyr Glu Thr561556205625Pro Leu Val Pro Val Val Ser Thr Val Thr Gly Ala Pro Ala Thr563056355640Pro Glu Glu Leu Arg Ser Pro Glu Tyr Trp Val Gly Gln Val Arg564556505655
Ala Thr Val Arg Phe Ala Asp Ala Val Arg Thr Leu Ala Arg Gln566056655670Gly Val Thr Ser Cys Leu Glu Leu Gly Pro Asp Gly Thr Leu Ser567556805685Ala Ala Ala Arg Asp Val Leu Asp Ala Asp Pro Ala Asp Ala Thr569056955700Gly Ala Arg Pro Val Thr Val Val Pro Ala Leu Arg Arg Asp Arg570557105715Asp Glu Val Pro Phe Leu Thr Ala Ala Leu Ala Arg Leu His Val572057255730His Gly Thr Ala Val Asp Trp Thr Gly Ala Phe Glu Gly Thr Gly573557405745Ala Arg Arg Val Asp Leu Pro Thr Tyr Ala Phe Gln Arg Ser Arg575057555760Tyr Trp Pro Asp Thr Thr Phe Thr Pro Ala Thr Ala Ala Gly Pro576557705775Ala Asp Thr Gly Ala Asp Ala Ala Phe Trp Ser Ala Val Glu Arg578057855790Ala Asp Leu Pro Thr Leu Gly Ala Asp Leu Gly Leu Asp Asp Asp579558005805Thr Leu Ser Ala Leu Val Pro Ala Leu Ser Ser Trp Arg Arg Arg581058155820Arg Thr Ala Gln Ala Ala Ala Asp Gly Arg Arg His Arg Ile Val582558305835Trp Lys Pro Leu Asp Gly Ala Pro Thr Gly Arg Pro Glu Gly Thr584058455850Trp Leu Val Leu Arg Pro Thr Gly Thr Ala Ala Pro Glu Thr Ala585558605865Ala Leu Leu Asp Thr Leu Gly Leu Asp Thr Ala Glu Val Thr Val587058755880Asp Leu Ala Ala Pro Asp Arg Ala Ala Leu Ala Asp Thr Leu Arg588558905895Arg Leu Pro Arg Pro Glu Asp Gly Phe Thr Gly Val Leu Ala Leu590059055910Pro Ala Leu Asp Thr Ala Ala Thr Ala Asp Asp Val Ala Gly Thr591559205925Gly Ala Val Ala Pro Thr Ala Ala Ala Leu Asn Ala Leu Asp Asp593059355940Ala Gly Ile Thr Ala Pro Leu Trp Ile Val Thr Arg Gln Ala Val594559505955Ser Thr Gly Arg Ala Asp Arg Leu Ala Arg Pro Gly Gln Ala Gly596059655970Val Trp Gly Leu Gly Arg Val Ala Ala Leu Glu Asn Pro Gly Arg597559805985
Arg Leu Ala Leu Val Asp Leu Pro Glu Gln Phe Asp Thr Arg Val599059956000Ala Arg Arg Leu Ala His Leu Leu Ala Val Pro Gly Thr Glu Asn600560106015Gln Leu Ala Val Arg Ala Ser Ala Thr Tyr Ala Arg Arg Ile Ala602060256030His His Pro Ala Pro Gln Glu Pro Ala Arg Pro Phe Ala Pro Glu603560406045Gly Thr Val Leu Ile Thr Gly Gly Thr Gly Ala Leu Gly Ala His605060556060Val Ala Arg Leu Leu Ala Gly Arg Gly Thr Pro His Leu Leu Leu606560706075Thr Ser Arg Arg Gly Ala Asp Ala Pro Gly Ala Ala Glu Leu Ala608060856090Ala Glu Leu Arg Glu Leu Gly Ala Glu Val Thr Leu Ala Ala Cys609561006105Asp Thr Ala Asp Arg Glu Ala Leu Ala Ala Leu Leu Ala Glu Val611061156120Pro Ala Asp Arg Pro Leu Thr Thr Val Val His Ala Ala Gly Val612561306135Leu Asp Asp Gly Leu Leu Asp Thr Leu Thr Pro Glu Arg Phe Ala614061456150Ala Val Leu Asp Ala Lys Ala Arg Ser Ala Ala His Leu His Glu615561606165Leu Thr Arg Asp Leu Gly Leu Thr Asp Phe Val Leu Phe Ser Ser617061756180Thr Ala Gly Thr Leu Gly Ala Ala Gly Gln Ala Asn Tyr Ala Ala618561906195Ala Asn Ala Tyr Leu Asp Ala Leu Ala Glu His Arg Arg Glu Gln620062056210Gly Leu Pro Ala Thr Ser Val Ala Trp Gly Pro Trp Ser Gly Thr621562206225Gly Met Ala Gly Glu Gly Thr Gly Val Glu Asp Arg Val Arg Arg623062356240Gly Gly Phe Thr Pro Met Ser Pro Glu Asp Ala Leu Ala Ala Leu624562506255Asp Ala Ala Val Gly His Arg Asp Thr Ala Leu Leu Val Ala Asp626062656270Ile Asp Trp Gln Arg Tyr Ala Thr Val Phe Ala Pro His Arg Ala627562806285Leu Val Gly Asp Leu Pro Glu Val Arg Ala Thr Val Gly Ala Ala629062956300Thr Ala His Gly Asp Thr Glu Pro Ala Leu Arg Arg Gln Val Ala630563106315
Ala Leu Thr Gly Pro Ala Arg Glu Arg Tyr Val Leu Asp Phe Leu632063256330Arg Ala Gln Val Ala Ala Val Leu Gly His Pro Asp Pro Asp Ala633563406345Val Glu Ala Asp Gln Ala Phe Thr Asp Leu Gly Phe Asp Ser Leu635063556360Thr Thr Val Glu Leu Arg Asn Thr Leu Gly Ala Thr Thr Gly Leu636563706375Arg Leu Pro Ala Thr Leu Val Tyr Asp His Pro Thr Ala Arg Asp638063856390Leu Ala Ala His Leu Leu Thr Glu Ile Leu Gly Asp Leu Pro Glu639564006405Pro Ala Ala Gly Gly Asn Ala Ala Gly Glu Ala Pro Arg Ala Thr641064156420Glu Thr Asp Pro Val Val Val Val Gly Met Gly Cys Arg Phe Pro642564306435Gly Gly Val Glu Ser Pro Glu Glu Leu Trp Gln Leu Leu Gly Glu644064456450Gly Arg Asp Ala Val Ser Gly Phe Pro Ala Asp Arg Gly Trp Asp645564606465Leu Asp Ser Leu Ala Arg Gly Gly Ser Ala Thr Leu Glu Gly Gly647064756480Phe Leu Gln Gly Ala Gly Leu Phe Asp Ala Ser Phe Phe Gly Ile648564906495Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu650065056510Leu Glu Thr Ser Trp Glu Ala Leu Glu Arg Ala Gly Ile Asp Pro651565206525Thr Gly Leu Arg Ser Ser Ala Thr Gly Val Phe Val Gly Thr Asn653065356540Gly Gln Asp Tyr Ala Thr Val Leu Arg Arg Gly Thr Thr Asp Val654565506555Arg Gly His Ala Ala Thr Gly Thr Thr Ala Ser Val Met Ser Gly656065656570Arg Leu Ser Tyr Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Val657565806585Asp Thr Ala Cys Ser Ser Ala Leu Val Ala Leu His Met Ala Ala659065956600Gly Ala Leu Arg Ser Gly Glu Cys Thr Leu Ala Leu Ala Gly Gly660566106615Val Ser Val Met Ala Ser Pro Asp Ala Phe Val Glu Phe Thr Ala662066256630Gln Gly Gly Leu Ala Gly Asp Gly Arg Cys Lys Ala Phe Ala Asp663566406645
Ala Ala Asp Gly Thr Ala Trp Gly Glu Gly Ala Gly Val Leu Val665066556660Leu Glu Arg Leu Ser Asp Ala Arg Arg His Gly His Pro Val Leu666566706675Ala Val Leu Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn668066856690Gly Leu Thr Ala Pro Asn Gly Arg Ala Gln Gln Arg Val Ile Arg669567006705Gln Ala Leu Ala Asp Ala His Leu Thr Pro Ala Glu Val Asp Ala671067156720Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu672567306735Ala His Ala Leu Ile Ala Ala Tyr Gly Glu Gly Arg Asp Pro Glu674067456750Gln Pro Leu Leu Leu Gly Thr Val Lys Ser Asn Leu Gly His Thr675567606765Gln Ala Ala Ala Gly Ala Ala Gly Val Ile Lys Thr Val Leu Ala677067756780Leu Gly His Gly Glu Leu Pro Arg Thr Leu His Val Asp Ala Pro678567906795Ser Ser His Val Asp Trp Ser Asp Gly Thr Val Glu Leu Leu Arg680068056810Glu His Arg Ala Trp Pro Glu Thr Gly Arg Ala Arg Arg Ala Gly681568206825Val Ser Ala Phe Gly Val Ser Gly Thr Asn Ala His Val Ile Leu683068356840Glu Gln Pro Glu Pro Ala Thr Ala Glu Thr Pro Glu Pro Val Thr684568506855Glu Pro Ser Val Val Pro Trp Leu Val Ser Ala Arg Ser Glu Asp686068656870Ala Leu Ala Ala Gln Thr Ala Arg Leu Thr Ala Phe Thr Gln Ala687568806885Arg Pro Glu Val Pro Ala Leu Asp Thr Ala Tyr Ser Leu Ala Thr689068956900Gly Arg Gly Ala Phe Ala His Arg Ala Val His Leu Val Thr Ala690569106915Gly Gly Glu Pro Leu Glu Thr Val Arg Asp Arg Ala Trp Glu Arg692069256930Arg Leu Ala Leu Leu Phe Ser Gly Gln Gly Ser Gln Arg Ala Gly693569406945Met Gly Arg Glu Leu Tyr Glu Arg Phe Pro Val Phe Ala Glu Ala695069556960Leu Asp Ser Val Val Ala Arg Leu Asp Thr Gly Leu Glu Arg Ser696569706975
Leu Arg Glu Val Leu Phe Ala Glu Glu Gly Ser Glu Ala Ala Ala698069856990Leu Leu Glu Ala Thr Gly Tyr Thr Gln Pro Ala Leu Phe Ala Val699570007005Glu Val Ala Leu Phe Arg Leu Val Glu Ser Trp Gly Val Ala Pro701070157020Glu Phe Val Ala Gly His Ser Val Gly Glu Ile Ala Ala Ala His702570307035Val Ala Gly Val Phe Ser Leu Asp Asp Ala Cys Ala Leu Val Ala704070457050Ala Arg Ala Arg Leu Met Gln Glu Leu Pro Thr Gly Gly Ala Met705570607065Val Ala Val Arg Ala Thr Glu Ala Glu Val Val Pro Arg Leu Thr707070757080Glu Gly Leu Ser Leu Ala Ala Val Asn Gly Pro Asp Ser Val Val708570907095Ile Ala Gly Glu Gln Glu Glu Val Leu Ala Leu Ala Ala Glu Phe710071057110Ala Gly Glu Gly His Lys Thr Gln Pro Leu Pro Val Ser His Ala711571207125Phe His Ser Ala Leu Met Glu Pro Met Leu Ala Glu Phe Arg Arg713071357140Val Ala Glu Ser Leu Glu Tyr Ala Glu Pro Arg Val Pro Val Val714571507155Ser Asn Val Thr Gly Ala Leu Ala Gln Pro Gly Gln Leu Thr Asp716071657170Pro Glu Tyr Trp Val Arg His Val Arg Glu Thr Val Arg Phe Ala717571807185Asp Gly Val Arg Ala Leu Ala Glu Ala Gly Ala Asp Ala Tyr Leu719071957200Glu Ile Gly Pro Asp Gly Val Leu Thr Gly Met Ala Ala Arg Val720572107215Leu Asp Thr Ala Ala Asp Gly Ser Gly Gly Val Ser Val Pro Ala722072257230Leu Arg Lys Asp Arg Pro Glu Glu His Ser Leu Leu Thr Ala Leu723572407245Ala Arg Leu His Thr Ala Gly Val Ala Val Asp Trp Thr Ala Trp725072557260Phe Thr Gly Thr Gly Ala Arg Arg Thr Asp Leu Pro Thr Tyr Ala726572707275Phe Gln Arg Glu Leu Tyr Trp Pro Glu Pro Ala Thr Gly Pro His728072857290Thr Ala Thr Ala Gln Asp Pro Ala Asp Ala Ala Phe Trp Ala Ala729573007305
Val Glu Arg Glu Asp Leu Glu Ser Leu Ser Ala Thr Leu Asp Leu731073157320Asp Asp Ala Val Leu Ser Asn Val Leu Pro Ala Leu Ser Thr Trp732573307335Arg Arg Gly Leu Ser Glu Arg Thr Leu Leu Asp Gly Trp Arg Tyr734073457350Arg Val Thr Trp Gln Pro Val Pro Leu Gly Asn Thr Ala Asp Asp735573607365Gly Thr Gly Arg Pro Trp Leu Val Leu Thr Leu Ala Gly His Asp737073757380Asp Ala Trp Thr Gln Ala Leu Ala Ala Thr Phe Gly Glu His Thr738573907395Val His Leu Ala Val Pro Ala Asp Glu Asp Gly Thr Ala Ala Leu740074057410Leu Asp Gly Ala Ala Asp Gly Thr Ala Tyr Ala Gly Val Leu Ser741574207425Leu Leu Ala Gly Thr Ala Thr Asp Gly Thr Ala Arg Pro Asp Thr743074357440Leu Leu Arg Leu Leu Ala Ala Ala Gly Ile Asp Ala Pro Leu Trp744574507455Cys Val Thr Arg Asp Ala Val Ser Val Gly Arg Ser Asp Pro Ala746074657470Ala Asp Pro Asp Arg Ala Ala Leu Trp Gly Leu Gly Arg Val Leu747574807485Ala Leu Asp Glu Pro Glu Arg Trp Gly Gly Leu Val Asp Val Asp749074957500His Val Ala Asp Ala Arg Thr Val Asp Arg Leu Arg Ala Val Leu750575107515Thr Val Ala Arg Thr Asp Gly Pro Ala Glu Asp Gln Val Ala Leu752075257530Arg Ala Ser Gly Ala Phe Gly Arg Arg Leu Ser Arg Ala Ala Ala753575407545Pro Ala Pro Asp Ile Thr Trp His Pro Ala Gly Thr Val Leu Val755075557560Thr Gly Arg Ala Glu Gly Phe Gly Gly His Val Ala Arg Trp Leu756575707575Ala Ala His Gly Ala Thr Gly Val Leu Leu Ala Gly Pro Ala Gly758075857590Pro Glu Asp Ala Ala Thr Thr Ala Leu Arg Ala Glu Val Glu Ala759576007605Leu Gly Ala Thr Leu Thr Val Val Arg His Pro Asp Pro Ala Asp761076157620Ala Ala Pro Leu Thr Glu Ala Leu Ala Ala Gln Ala Asp Asp Arg762576307635
Pro Leu Thr Ala Val Leu His Thr Gly Asp Thr Gly Glu Glu Thr764076457650Pro Gly Val Thr Gly Ala Arg Ala Ala Tyr Glu Ala Leu Thr Thr765576607665Ala Val Gly Asp Arg Asp Leu Asp Ala Phe Val Val Phe Gly Ser767076757680Ile Ser Ala Val Trp Gly Val Gly Gly Gln Gly Ala Gly Ala Ala768576907695Ala Gly Val Cys Leu Asp Ala Leu Val Gln Arg His Arg Ala His770077057710Gly Thr Asn Ala Val Ser Val Ser Trp Gly Ala Trp Gln Gly Ala771577207725Gly Pro Asp Gly Leu Ala Ala His Leu Arg Ala Asn Gly Leu Pro773077357740Ala Met Glu Pro Ala Arg Ala Leu Asp Ala Leu Ala Pro Ala Ile774577507755Gly Glu Ala Ala Ala Asn Pro Ala Ala Pro Ala Ser Val Thr Val776077657770Ala Asp Val Ala Trp Asp Arg Phe Ala Pro Ala Phe Thr Arg Thr777577807785Arg Ala Gly Leu Leu Leu Thr Gly Val Pro Glu Ala Arg Glu Ala779077957800Leu Ser Thr Thr Gly Gly Asp Gly Ala Asp Ala Gly Thr Ala Ser780578107815Ala Leu Arg Glu Arg Leu Arg Gln Ser Asp Pro Ser Glu Arg Pro782078257830Arg Thr Leu Leu Asp Thr Val Leu Thr Glu Ile Ala Ser Val Leu783578407845Gly His Thr Gly Ala Ala Ala Val Pro Ala Glu Asn Ala Phe Asn785078557860Asp Leu Gly Phe Asp Ser Leu Thr Ala Val Asp Leu Arg Asn Arg786578707875Leu Thr Thr Ala Thr Gly Leu Thr Leu Pro Ala Thr Leu Val Phe788078857890Asp Tyr Pro Thr Pro Ala Ala Leu Ala Ala His Leu Leu Thr Glu789579007905Leu Leu Gly Glu Asp Thr Gly Pro Gly Thr Gly Ala Pro Val Ala791079157920Arg Ala Ala Ala Asp Ala Asp Asp Pro Val Val Ile Val Gly Met792579307935Ser Cys Arg Tyr Pro Gly Asp Val Arg Ser Pro Glu Asp Leu Trp794079457950Glu Leu Val Gly Ala Gly Thr Asp Ala Ile Gly Gly Phe Pro Thr795579607965
Asp Arg Gly Trp Asp Leu Glu Lys Leu Leu His Gly Asp Glu Asp797079757980Gly Arg Gly Arg Ser Val Thr Arg Glu Gly Gly Phe Leu Tyr Asp798579907995Val Ala Asp Phe Asp Pro Ala Phe Phe Gly Ile Ser Pro Arg Glu800080058010Ala Leu Val Ile Asp Pro Gln Gln Arg Ile Val Leu Gln Ala Ala801580208025Trp Glu Ala Leu Glu Arg Ala Gly Ile Asp Pro Ala Val Leu Arg803080358040Gly Gly Asp Thr Gly Val Phe Val Gly Gly Gly Ser Gly Asp Tyr804580508055Arg Pro Ala Ile Gly Gln Ser Gly His Val Glu Thr Ala Gln Ser806080658070Ala Ser Leu Leu Ser Gly Arg Leu Ser Tyr Thr Leu Gly Leu Glu807580808085Gly Pro Ser Val Ser Val Asp Thr Ala Cys Ser Ser Ser Leu Thr809080958100Ala Leu His Leu Ala Ala Gln Ala Ile Arg Ser Gly Glu Cys Ser810581108115Leu Ala Leu Ala Gly Gly Val Thr Val Met Ser Thr Pro Val Gly812081258130Phe Val Glu Phe Gly Glu Met Gly Ala Leu Ser Pro Asp Gly Arg813581408145Cys Lys Pro Phe Ser Asp Ala Ala Asp Gly Thr Ala Trp Ser Glu815081558160Gly Val Gly Met Leu Val Val Glu Arg Leu Ser Glu Ala Arg Arg816581708175Arg Gly His Glu Val Leu Ala Val Leu Arg Gly Ser Ala Ile Asn818081858190Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser819582008205Gln Gln Arg Val Ile Arg Lys Ala Leu Ala Ala Ala Gly Leu Thr821082158220Ala Arg Glu Val Asp Ala Val Glu Ala His Gly Thr Gly Thr Thr822582308235Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly824082458250Gln Asp Arg Pro Glu Gly Gln Pro Leu Leu Leu Gly Ser Val Lys825582608265Ser Asn Ile Gly His Ser Gln Ala Ala Ser Gly Val Ala Gly Ile827082758280Ile Lys Met Val Leu Ala Met Arg His Gly Thr Leu Pro Arg Thr828582908295
Leu His Ala Glu Lys Pro Ser Arg His Val Ala Trp Asp Pro Asp830083058310Ala Val His Leu Leu Thr Glu Ala Ala Asp Trp Pro Glu Thr Gly831583208325Arg Pro Arg Arg Ala Ala Val Ser Ser Phe Gly Ala Ser Gly Thr833083358340Asn Ala His Val Ile Leu Glu Gln Ala Pro Thr Gly Pro Ala Ala834583508355Pro Glu Arg Asp Thr Glu Pro Ala Thr His Ala Thr Asp Ala Ala836083658370Arg Leu Leu Pro Leu Pro Leu Ser Ala Ala Thr Ala Asp Ser Leu837583808385Thr Glu Gln Ala Ala Arg Leu Arg Asp Arg Leu Thr Ala Gly Ala839083958400Ala Gln Pro Pro Ala Thr Ala Asp Leu Ala Leu Ser Leu Gly Thr840584108415Thr Arg Ser Ala Phe Glu His Arg Ala Val Leu Ala Val Ala Gly842084258430Arg Asp Asp Leu Leu Asp Ala Leu Thr Ala Leu Ala Glu Asp Arg843584408445Ala His Pro Ala Val Leu Arg Gly Arg Ala Ala Pro Gly Gly Arg845084558460Thr Ala Phe Leu Phe Pro Gly Gln Gly Ser Gln Arg Pro Gly Ala846584708475Gly Arg Ala Leu Tyr Ala Arg Phe Pro Val Phe Ala Thr Ala Leu848084858490Asp Glu Val Leu Ala His Phe Asp Gln Glu Leu Glu Arg Pro Leu849585008505Arg Asp Val Met Phe Ala Asp Glu Gly Thr Pro Glu Ala Ala Leu851085158520Leu Asp Arg Thr Gly Trp Thr Gln Pro Ala Leu Phe Ala Leu Gly852585308535Val Ala Leu Tyr Arg Leu Val Glu Ser Trp Gly Val Arg Pro Asp854085458550Leu Leu Ala Gly His Ser Val Gly Glu Leu Ala Ala Ala His Val855585608565Ala Gly Val Leu Ser Leu Pro Asp Ala Cys Arg Val Val Ser Ala857085758580Arg Ala Arg Leu Met Glu Ala Leu Pro Ala Gly Gly Ala Met Leu858585908595Ala Val Arg Ala Thr Glu Asp Glu Ile Ala Pro Tyr Leu Thr Asp860086058610Gln Ile Cys Leu Ala Ala Val Asn Gly Pro Glu Ser Val Val Val861586208625
Ser Gly Asp Glu Lys Ala Val Thr Ala Leu Ala Glu Ala Leu Ala863086358640Glu Gln Gly Arg Lys Thr Arg Arg Leu Arg Val Ser His Ala Phe864586508655His Ser Leu His Met Asp Ala Met Leu Gln Asp Phe Glu Arg Val866086658670Thr Arg Ser Val Ser Tyr Ala Pro Pro Thr Leu Pro Leu Val Ser867586808685Asn Leu Thr Gly Asp Ala Ala Thr Asp Ala Gln Val Cys Asp Pro869086958700Gly Tyr Trp Val Arg His Val Arg Glu Ala Val Arg Phe Gly Asp870587108715Gly Val Arg Thr Leu Ala Gly Arg Gly Ala Thr Arg Tyr Leu Glu872087258730Leu Gly Pro Asp Gly Ile Leu Cys Gly Leu Ala Gln Glu Thr Leu873587408745Asp Thr Leu Ala Asp Glu Asn Arg Pro Ala Pro Thr Ala Val Pro875087558760Ala Leu Arg Thr Gly Arg Asp Glu Glu Gln Ala Leu Ile Thr Ala876587708775Val Ala Arg Leu His Ala Ala Gly Gln Gly Leu Asp Trp Ala Ala878087858790Leu Leu Asp Gly Thr Gly Ala Arg Arg Val Asp Leu Pro Thr Tyr879588008805Pro Phe Arg Arg Leu Arg Phe Trp Pro Asp Glu Ala Pro Ala Ala881088158820Ala Thr Gly Thr Thr Ala Asp Ala Gly Asp Ala Ala Phe Trp Ala882588308835Ala Val Gln Asp Glu Gln Phe Asp Ser Leu Ala Gly Glu Leu Gly884088458850Val Asp Gly Asp Ala Leu Leu Arg Val Leu Pro Ala Leu Arg Asp885588608865Trp Arg Arg Lys His Gly Asp Gln Thr Thr Val Asp Gly Trp Arg887088758880Gln Arg Ile Ala Trp Arg Pro Leu Asn Arg Ala Thr Ala Gly Thr888588908895Pro Ala Gly Thr Trp Leu Ala Val Val Pro Ala Asp His Ala Asp890089058910His Pro Trp Thr Ser Ala Val Leu Ala Ala Leu Gly Ala Gly Ala891589208925Gln Val Leu Glu Val Ser Gly Lys Asp Arg Ala Gly Leu Ala Ala893089358940Ala Ile Arg Glu Arg Leu Asp Ala Thr Gly Pro Phe Thr Gly Val894589508955
Val Ser Leu Leu Ala Val Ala Gly Glu Pro Gly Asp Gly Thr Gly896089658970Ala Leu Pro Gly Gly Val Pro Ala Gly Ala Ala Leu Thr Ala Thr897589808985Leu Val Gln Ala Leu Gly Asp Ala Arg Thr Asp Ala Pro Leu Trp899089959000Cys Leu Thr Arg Gly Ala Ala Ala Val Ser Pro Ala Glu Thr Val900590109015Ala Ala Pro Leu Gln Ala Ala Val His Gly Leu Gly Arg Val Ala902090259030Ala Leu Glu His Pro Gln Arg Trp Gly Gly Thr Val Asp Leu Pro903590409045Leu Ala Val Asp Ala Arg Thr Ala Glu Arg Leu Ala Ala Val Leu905090559060Ala Asp Pro Glu Gly Glu Asp Gln Leu Ala Val Arg Pro Gly Ala906590709075Val Phe Gly Arg Arg Leu Ala Ala Val Arg Pro Gly Thr Pro Arg908090859090Asp Trp Lys Pro Thr Gly Thr Val Leu Ile Thr Gly Gly Thr Gly909591009105Ala Leu Gly Ala Arg Thr Ala Arg Arg Leu Ala Gly Ala Gly Ala911091159120Arg Arg Leu Val Leu Leu Ser Arg Ser Gly Pro Asp Ala Pro Gly912591309135Ala Gly Glu Leu Ala Ala Asp Leu Arg Ala Leu Gly Ala Glu Pro914091459150Val Ile Thr Ala Cys Asp Thr Ala Asp Arg Lys Ala Leu Ala Ala915591609165Val Leu Ala Ala Ile Pro Glu Asp Ala Pro Leu Thr Gly Val Ile917091759180His Thr Ala Gly Val Leu Asp Asp Gly Val Val Asp Gly Leu Thr918591909195Pro Glu Arg Phe Ala Thr Val Phe Arg Ala Lys Val Ala Ser Ala920092059210Leu Leu Leu Asp Glu Leu Thr Arg Ala His Asp Leu Glu Val Phe921592209225Ala Leu Phe Ser Ser Ala Ser Ala Ala Val Gly Asn Pro Gly Gln923092359240Gly Thr Tyr Ala Ala Ala Asn Ala Val Leu Asp Ala Leu Ala Glu924592509255Gln Arg Arg Ala Glu Gly Leu Pro Ala Thr Ser Val Ala Tyr Gly926092659270Ala Trp Gly Gly Glu Gly Met Ala Asp Gly Val Arg Ala Ala Ala927592809285
Leu Ala Arg Arg Thr Gly Ile Arg Pro Leu Asp Pro Asp Leu Ala929092959300Val Leu Ala Leu Arg Gln Val Val Thr Gly Ser Asp Pro Val Ala930593109315Val Val Ala Asp Val Asp Pro Asp Arg Phe Val Arg Ala Phe Thr932093259330Thr Val Arg Pro Ser Ser Leu Leu Ala Glu Met Pro Ala Tyr Ala933593409345Ala Leu Lys Lys Ala Ala Ser Ala Gly Gly Glu Ala Ala Asp Ser935093559360Gly Pro Ser Leu Arg Asp Arg Leu Ala Arg Leu Pro Glu Ser Arg936593709375Arg Thr Gln Thr Val Leu Thr Leu Val Arg Glu Arg Ala Ala Glu938093859390Val Leu Gly Leu Thr Gly Thr Asp Gln Val Gly Pro Asp Arg Ala939594009405Phe Arg Asp Leu Gly Phe Asp Ser Leu Gly Ala Val Glu Leu Arg941094159420Asn Gln Leu Gly Ala Val Ser Gly Leu Thr Leu Ser Ala Thr Leu942594309435Val Phe Asp His Pro Thr Pro Ala Ala Leu Ala Ala His Ile Leu944094459450Gly Gln Leu Leu Pro Ala Gly Thr Pro Gly Ala Pro Ala Thr Asp945594609465Gly Ala Gly Glu Glu Glu Arg Ala Val Arg Ala Ala Leu Ala Gln947094759480Val Pro Leu Asp Arg Leu Arg Asp Ser Gly Leu Leu Asp Gln Leu948594909495Leu Asp Leu Ala Gly Gln Asp Pro Ala Gly Thr Gly Thr Asp Gln950095059510Glu Ala Glu Pro Ser Gly Ala Gly Asp Ala Tyr Asp Ala Ser Ile951595209525Asp Ala Met Asp Val Asp Gly Leu Val Gln Ala Ala Leu Asn Gly953095359540Asn Pro Asp Glu Glu Arg Asp95459550<210>8<211>335<212>PRT<213>鏈霉菌FR-008<400>8
Met Asn Gly Leu His Leu Pro Pro Ala Pro Leu Pro Ala Pro Gly Val1 5 10 15Ser Ala Ala Gly Ser Gly Asp Leu Met Ile Glu Ala Glu Gly Val Ser20 25 30Lys Ala Tyr Gly Thr Val His Ala Leu Asp Ser Val Ser Leu Ala Val35 40 45Pro Arg Gly Thr Val Leu Gly Leu Leu Gly His Asn Gly Ala Gly Lys50 55 60Thr Thr Leu Val Asp Ile Leu Thr Thr Ala Leu Pro Pro Thr Ser Gly65 70 75 80Arg Ala Arg Val Ala Gly Tyr Asp Val Ala Asp Lys Pro Val Glu Ile85 90 95Arg Arg Arg Ile Gly Leu Thr Gly Gln Phe Ala Ser Val Asp Ala Gln100 105 110Leu Ser Gly Tyr Asp Asn Leu Val Leu Ile Ala Arg Leu Leu Gly Ala115 120 125Gly Arg Arg Ala Ala Arg Val Arg Ala Arg Glu Leu Leu Glu Leu Phe130 135 140Arg Leu Thr Glu Val Ala Asp Arg Arg Ala Ser Ser Tyr Ser Gly Gly145 150 155 160Leu Arg Arg Arg Leu Asp Leu Ala Val Ser Leu Val Gly Ala Pro Glu165 170 175Val Leu Phe Leu Asp Glu Pro Thr Thr Gly Leu Asp Pro Ser Ser Arg180 185 190Ile Asn Leu Trp Glu Ile Val Glu Gly Leu Val Glu Gln Gly Thr Thr195 200 205Val Leu Leu Thr Thr Gln Tyr Leu Glu Glu Ala Asp Arg Leu Ala Asp210 215 220Arg Ile Ala Val Leu Ser Ala Gly Arg Val Val Ala Ala Gly Thr Ala225 230 235 240Pro Glu Leu Lys Ala Thr Val Gly Arg Arg Thr Val Thr Leu Thr Leu245 250 255Glu Thr Gly Glu Asp Val Ser Ala Ala Arg Thr Ala Leu Arg Gly Ala260 265 270Gly Phe Ala Pro Val Asp Gly Glu Ala Arg Thr Val Val Val Pro Ile275 280 285Asp Ala Thr Arg Glu Ile Ala Asp Ile Ile Arg Ser Leu Asp Gln Ala290 295 300Gly Val Glu Ala Ser Glu Leu Asn Phe Gly Glu ProThr Leu Asp Asp305 310 315320Val Tyr Leu Thr Leu Ala Glu Gln Ala Ala Gly Arg Thr Arg Ala325 330 335
<210>9<211>239<212>PRT<213>鏈霉菌FR-008<400>9Met Val Thr Asp Pro Gly Ile Val Leu Phe Gly Leu Ile Gln Pro Val1 5 10 15Val Ile Leu Phe Val Leu Thr Gln Val Phe Ser Lys Met Gly Met Pro20 25 30Pro His Phe Pro Asp Gly Val Ser Tyr Leu Asp Tyr Val Leu Pro Ala35 40 45Val Leu Val Asp Asn Ala Ala Gln Ser Ala Met Gln Ser Gly Val Gly50 55 60Leu Val Glu Asp Gln Lys Asn Gly Ile Val Ala Arg Leu Arg Ser Leu65 70 75 80Pro Val His Pro Gly Ala Leu Leu Ala Ala Arg Ser Leu Val Gly Leu85 90 95Val Arg Ser Ala Val Gln Val Ala Val Ile Met Ala Leu Ala Met Thr100 105 110Val Leu Gly Tyr Ser Pro Asn Gly Gly Ala Ala Glu Leu Ala Leu Ser115 120 125Ala Gly Leu Thr Leu Phe Ile Ser Phe Ser Leu Gly Trp Ala Phe Ile130 135 140Ala Ala Gly Ala Trp Leu Arg Arg Ala Glu Pro Leu Gln Asn Leu Ala145 150 155 160Leu Ile Val Ile Phe Pro Leu Met Phe Ala Ser Ser Ala Tyr Val Pro165 170 175Val Ala Asp Leu Pro Ser Trp Leu Gly Ala Val Ala Ser Val Asn Pro180 185 190Leu Thr Tyr Ala Ile Asp Ala Thr Arg Ala Leu Ala Leu Asp Val Pro195 200 205Gly Leu Asp His Ala Ile Pro Ala Val Val Ile Cys Ala Val Ile Ala210 215 220Val Val Gly Met Ile Ala Ala Val Arg Gly Phe Arg Arg Pro Leu225 230 235<210>10<211>222<212>PRT<213>鏈霉菌FR-008<400>10
Met Asp Pro Ala Pro Ala Ala Asp Pro Ala Gly Ser Pro Glu Arg Ala1 5 10 15Ala Val Leu Ala Asp Gly Phe Asp Arg Ala Gly Ala Tyr Val Ala Cys20 25 30Leu Asp Pro Ser Leu Thr Ile Gln Gln Val Asn Gln Glu Phe Glu Arg35 40 45Arg Phe Gly Gly Ser Ser Ser Glu Leu Cys Gly Ser Arg Phe Cys Asp50 55 60Leu Val His Pro Ser Val Gln Gln Pro Leu Met His Gln Phe Ala Arg65 70 75 80Met Leu Asp Gly Lys Arg His Arg Phe Ala Thr Glu Val Ile Ala Val85 90 95Asp Gln Glu Arg Thr Ala Ser Thr Leu Pro Leu Asn Ala Leu Ala Val100 105 110Arg Gly Gly Arg Thr Pro Asp Val Ala Ala Ile Leu Val Val Met Asn115 120 125Ala Ala Glu Glu Glu Ala Gly Asp Ala Asp Val Met Ala Pro Arg Lys130 135 140Lys Leu Leu Ser Glu Ile Asp Ala Arg Ile Leu Glu Gly Ile Ala Ala145 150 155 160Gly Val Ser Thr Ile Pro Leu Ala Ser Arg Leu Tyr Leu Ser Arg Gln165 170 175Gly Val Glu Tyr His Val Thr Gly Leu Leu Arg Lys Leu Lys Val Pro180 185 190Asn Arg Ala Ala Leu Val Ser Arg Ala Tyr Ser Met Gly Val Leu Lys195 200 205Val Gly Thr Trp Pro Pro Lys Val Val Glu Asp Phe Ile Lys210 215 220<210>11<211>942<212>PRT<213>鏈霉菌FR-008<400>11Met Leu Leu Glu Arg Thr Thr Glu Ile Ala Leu Val Asp Ala Ala Leu1 5 10 15Gly Ala Ala Thr Glu Gly Arg Ser Ser Leu Val Leu Leu Thr Gly Pro20 25 30Leu Gly Ile Gly Arg Ser Ala Leu Leu Gln Arg Leu Ser Cys Leu Ala35 40 45Asp Asp Arg Asp Asp Val Arg Val Leu Arg Ala His Ala Ala Pro Met50 55 60
Glu Gln Asp Phe Ala Phe Gly Val Val Arg Gln Leu Phe Glu Thr Leu65 70 75 80Leu Gly Asp Ser Pro Glu Asp Ala Arg Asp Arg Trp Met Asp Ala His85 90 95Ala Ser Phe Ala Arg His Val Leu Ala Asp Asp Ala Ala Pro Pro Gly100 105 110Ala Asp Gln Ala Ile Ala Ala Thr Glu Ala Val Leu His Gly Leu Leu115 120 125Ser Leu Leu Ala Asn Val Ser Ala Asp Ser Arg Leu Leu Ile Leu Val130 135 140Asp Asp Leu Gln Trp Ser Asp Val Pro Ser Leu Arg Trp Leu Thr Tyr145 150 155 160Leu Ala Lys Arg Leu His Gly Leu Arg Ala Val Val Val Cys Ala Leu165 170 175Arg Asp Gly Asp Pro Arg Ser His His Thr Leu Ala Arg Glu Ile Arg180 185 190Glu Ala Gly Thr Gln Thr Leu Arg Pro Ala Ser Leu Ser Leu Thr Ala195 200 205Thr His Glu Leu Val Arg Glu His Phe Gly Glu Ala Gly Asp Asp Glu210 215 220Phe Val Gln Ala Cys His Glu Ala Ser Val Gly Asn Pro Leu Phe Leu225 230 235 240Leu Ser Ile Leu Val Gly Thr Gly Phe Leu Gly Arg Arg Pro Leu Ala245 250 255Glu His Ala Glu Thr Ala Arg Arg Leu Arg Pro Ser Gln Leu Arg Glu260 265 270Arg Leu Ala Ser Ile Leu Arg Thr Gln Pro Ala Pro Val Arg Asp Leu275 280 285Ala Ala Ala Ile Ala Ile Leu Gly Glu Gln Ser Asp Ala Pro Thr Leu290 295 300Ala Arg Leu Ala Gly Leu Asp Ser Ile Gly Tyr Ala Gly Ala Leu Arg305 310 315 320Ala Leu Gly Ala Leu Gly Ala Leu Ala Ala Pro Asp Glu Pro Arg Phe325 330 335Ile His Arg Ser Val Arg Asp Ala Ala Glu Ser Thr Leu Thr Met Val340 345 350Gln Arg Glu Arg Met His Asp Glu Ala Ala Ala Leu Leu Tyr Ala Ala355 360 365Gly Ser Pro Ala Glu Gln Val Ala Ala Gln Leu Met Ala Val Val Thr370 375 380Arg Arg Gln Pro Trp Ala Val Asp Val Leu Arg Ala Ala Ala Asp Thr385 390 395 400Ala Leu Arg Arg Gly Ala Pro Asp Thr Ala Ala Gly Tyr Leu Cys Arg405 410 415
Ala Leu Leu Asp Ser Pro Ala Ala Gly Val Gly Arg Gly Arg Leu Leu420 425 430Val Glu Leu Gly Thr Ala Glu Arg Gly Phe Asp Pro Leu Ala Cys Glu435 440 445Arg His Ile Ala Gln Ala Met Thr Leu Leu Pro Glu Pro Arg Asp Arg450 455 460Ala Val Ala Ala Leu Arg Ile Ser Pro Thr Ala Leu Gly Pro Ala Pro465 470 475 480Leu Thr Ala Val Asp Leu Leu Arg Gln Ala Ala Glu Asp Leu Gly Pro485 490 495Ala Asp Gly Leu Thr Gly Thr Asp Arg Asp Leu Ala Leu Arg Leu Glu500 505 510Ala Arg Leu Arg His Cys Gly His Glu Asp Pro His Glu Leu Ala Ala515 520 525Ser Ala His Arg Leu Lys Glu Leu Gly Pro Glu Pro Pro Val Asp Thr530 535 540Gly Gly Glu Arg Glu Leu Thr Ile Ala Leu Leu Gly Ala Ala Thr Leu545 550 555 560Gly Cys His Leu Pro Ala Ser Glu Val Val Arg Leu Ala Gly Arg Val565 570 575Leu Asp Arg Glu Pro Ala Thr Ser Ala Arg Ile His Ser Thr Leu Pro580 585 590Leu Ala Ile Ile Thr Met Ile Ala Ala Asp Ser Val Glu Ala Val Asp595 600 605Ser Trp Leu Ala Val Glu His Arg Ala Asn Glu Arg Gly Thr Thr Pro610 615 620Ala Asn Ala Leu Val His Ile Glu Gln Ala Leu Val His Leu Gly Arg625 630 635 640Gly Arg Leu Ala Gln Ala Arg Glu Gln Ser Glu Thr Ala Leu Gly Met645 650 655Thr Glu Pro Ala His Gln Gly Leu Asp Thr Ala Ala Thr Met Thr His660 665 670Ala Ile Val Ala Leu Glu Ser Arg Asp Pro Ser Leu Ala Glu Lys Val675 680 685Leu Arg Arg Ala Ser Arg Ile Arg Pro Gln Gly Leu Thr Leu Thr Gly690 695 700Ile Leu Arg Leu Leu Glu Ala Thr Arg Glu Ile Ala Ala Gly Asp Gln705 710 715 720Thr Gly Ala Leu Asp Ser Leu His Ser Cys Gly Arg Gln Leu Glu Ala725 730 735Ala Gly Trp His Asn Pro Val Leu Phe Pro Trp Arg Pro Trp Ala Val740 745 750Gly Val His Arg Arg Leu Gly Asp Ile Arg Thr Ala Arg Ser Leu Ala755 760 765
Glu Glu Glu Tyr Ala Arg Ala Glu Gln Trp Gly Ala Pro Val Gly Val770 775 780Gly Arg Ala Leu Arg Leu Leu Gly Arg Leu Glu Asp Gly Gly Arg Gly785 790 795 800Ile Gly Leu Leu Arg Glu Ala Val Thr Val Leu Arg Gly Ser Ala Asn805 810 815Gln Leu Glu Leu Ala Arg Ala Leu Ser Thr Leu Ala Glu Arg Leu Gly820 825 830Gly Gly Ala Glu Thr Glu Ala Leu Ala Arg Glu Ala Ala Thr Leu Ala835 840 845Thr Ser Cys Gly Ala Pro Gly Leu Ala Glu Arg Ala Ala Arg Gly Ala850 855 860Gly His Arg Ser Gly Pro Pro Ala Thr Pro Glu Asp Val Leu Thr Pro865 870 875 880Thr Glu Arg Arg Val Val Gly Leu Ala Cys Arg Gly Leu Thr Asn Gln885 890 895Glu Ile Ala Gly Ala Leu Gly Val Ser Ser Arg Ala Val Glu Lys His900 905 910Leu Thr His Ala Tyr Arg Arg Leu Gly Ile Ser Gly Arg Arg Glu Leu915 920 925Ile Val Leu Phe Ser Arg Ala Glu Tyr Ala Gly Thr Gly Asp930 935 940<210>12<211>1036<212>PRT<213>鏈霉菌FR-008<400>12Met Arg Ser Gly Glu Ile His Arg Lys Asn Ser Gly Leu Gln Thr Pro1 5 10 15Thr Cys Cys Thr Ala Gln Met Tyr Asp Leu His Met Pro Pro Ile Glu20 25 30Leu Pro Leu Leu Glu Arg Asp Arg Glu Leu Ala Ala Leu Ser Ala Val35 40 45Ile Gly Glu Leu Gly Ser Gly Arg Pro Ala Val Val Thr Val Thr Gly50 55 60Glu Pro Gly Leu Gly Gln Asn Asp Leu Leu Arg Trp Ala Ala Ala Tyr65 70 75 80Ala Thr Asp Ala Gly Leu Arg Val Leu Ser Ala His Ala Thr Pro Ala85 90 95Glu His Glu Val Arg Tyr Gly Val Val Ala Gln Leu Leu Ala Glu Glu100 105 110
Asn Arg Ala Leu Ala Pro Arg Leu Phe Leu Thr Asp Glu Gln Pro Gly115 120 125Gly Leu Pro Gly Leu Asn Gly Leu Leu Asn Ala Val Arg Asp Arg Pro130 135 140Thr Leu Leu Val Val Arg Asp Thr Gln Trp Leu Asp Pro Ala Ser Leu145 150 155 160Arg Trp Leu Glu Ala Leu Ala Arg Arg Leu Pro Arg Ala Pro Val Ala165 170 175Leu Leu Thr Ser Thr Thr Gly Thr Ala Leu Thr Arg Pro Glu Trp Ser180 185 190Val Gly Thr Pro Leu Thr Gly Leu Ala Thr Thr Ile Glu Leu Ala Leu195 200 205Pro Pro Leu Thr Ser Gly Gly Thr Ala Thr Ala Val Leu Arg Ala Phe210 215 220Gly Ala Pro Gly Asp Pro Ala Phe Thr Glu Ala Leu Ala Glu Ala Thr225 230 235 240Arg Gly Ile Pro Ala Val Val His Asp Val Leu Asp Arg Phe Ala Arg245 250 255Ala Gly Tyr Ser Pro Arg Ala Asp Arg Ile Asp Pro Leu Arg Ala Leu260 265 270Thr Ala Glu Val Val Gly Asp His Ala Thr Arg Ala Leu Ser Asp Leu275 280 285Arg Asp Pro Ala Ala Val Asp Ala Leu Arg Ala Leu Ala Val Cys Gly290 295 300Asp Leu Leu Asp Phe Pro Leu Val Cys Thr Leu Ala Gly Pro His Ser305 310 315 320Val Ser Glu Ser Arg Leu Arg Ala Ala Leu Ala Ala Ser Gly Leu Thr325 330 335Thr Leu Arg Asp Gly His Pro Arg Val Gln Asp Ala Val Val Arg Ala340 345 350Arg Val Leu Glu Glu Met Pro Ala Ala Asp Arg Ala Glu Leu Tyr Ala355 360 365Arg Ala Ala Gly Leu Ala Gln Arg Val Ala Ala Asp Asp Gln Gly Ile370 375 380Ala Asp Leu Leu Leu Leu Ala Arg Pro Val Gly Asp Pro Trp Ala Val385 390 395 400Asp Thr Leu Arg Arg Gly Phe Thr Ser Ala Leu Arg Gly Gly Arg Arg405 410 415Asp Leu Ala Val Ala Tyr Leu Ala Arg Ala Leu Asp Glu Pro Leu Ala420 425 430Ala Glu Asp Arg Ala Arg Ile Glu Phe Gln Leu Ala Ser Val Glu Met435 440 445Val Thr Ala Pro Thr Ala Ala Glu Arg Arg Leu Gly Gly Leu Ile Arg450 455 460
Ala Thr Arg Pro Gly Pro Gly Ala Gly Leu Arg Ala Arg Ala Thr Asp465 470 475 480Leu Cys Leu Leu Gly Gly Asp Thr Arg Ala Ala Arg His Ala Leu Ala485 490 495Gly Ala Ile Asp Ser Ala Pro Ala Ala Pro Glu Pro Arg Arg Gly Pro500 505 510Thr Gly Ala Pro Ala Val Pro Pro Glu Ala Glu Pro Ala Ser Thr Pro515 520 525Arg Thr His Leu Val Pro His Arg Pro Tyr Ala Gly Thr Pro Pro Gly530 535 540Ala Thr Gly Pro Asp Gly Arg Arg Ser Ala Ala Pro Asp Leu Pro Gly545 550 555 560Thr Pro Arg Ala His Asp Ala Pro Arg Ser Pro Gly Pro Glu Ala Ala565 570 575Gly Arg Pro Asp Thr Ala Gly Pro Asp Glu Asp Pro Ala Val Ala Glu580 585 590Arg His Glu Leu Thr Val Leu Cys Arg Val Ala Ser Phe Leu Arg His595 600 605Asp Asp Ala Asp Leu Asp Val Leu Pro Val Pro Ala Leu Pro Asp Ala610 615 620Val His Asn Pro Ala Val Ala Gly Val Trp Ala Trp Glu His Gly Val625 630 635 640Ala Gly Val His Arg Glu Glu Val Arg Arg Leu Ala Arg Ser Ala Phe645 650 655Val Pro Ala Ala Thr Gly Arg Pro Leu Leu Val Val Pro Arg Leu Leu660 665 670Gly Cys Arg Ala Leu Leu Leu Thr Asp Asp Gly Asp Glu Ala Glu Asn675 680 685His Leu Gly Ala Leu Leu Asp Glu Ser His Arg Glu Arg Ala Ala Ile690 695 700Ser Val Ala His Ile Leu Thr Val Arg Ala Glu Leu His Ile Arg His705 710 715 720Gly Arg Pro Asp Ala Ala Ala Arg Asp Leu Ala Ala Ala Gln Ala Glu725 730 735Leu Pro Leu Asp Arg Leu His Pro Leu Phe Leu Pro Tyr Trp Leu Ala740 745 750Leu Ser Met Ile Thr Asp Leu Gln Asn Gly His Thr Glu Arg Ala Arg755 760 765Glu Thr Ala Ala Arg Pro Leu Pro Pro Leu Ala His Glu Ser Ala Thr770 775 780Thr Ala Gln Leu Leu Phe Ala Arg Gly Val Leu Ala Arg Thr Asp Asp785 790 795 800Glu Pro His Gln Ala Arg Glu His Phe Arg Ala Cys Gly Arg Trp Leu805 810 815
Leu Arg His Gly Cys Ala Asn Pro Ala Val Gln Pro Trp Arg Ser Leu820 825 830Ala Ala Glu Ala Ala His Thr Leu Gly Asp Thr Glu Glu Ala Ala Arg835 840 845Leu Val His Glu Glu Val Arg Leu Ala Arg Arg Trp Gly Ala Ala Ala850 855 860Pro Leu Gly Arg Ala Gln Leu Ser Leu Ala Val Val Thr Glu Glu Asn865 870 875 880Arg Val Glu Asn Leu Arg Ala Ala Val Thr Thr Leu Ser Ala Ala Ser885 890 895Ala Arg Thr Ala Tyr Thr Arg Ala Val Ile Glu Leu Ala Ala Ala Glu900 905 910Arg Glu Glu Asn Glu Arg Arg Thr Ala Val Ala Leu Gly Pro Ala Ala915 920 925Thr Val Leu Pro Pro Val Ala Pro Pro Pro Gly Pro His Val Pro Ser930 935 940Ala Val Ala Arg Pro Pro Gly Pro Arg Pro Ala Val Leu Thr Gly Arg945 950 955 960Arg Thr Ala Lys Ala Pro Gly His Asp Ala Ala Val Trp His Gly Leu965 970 975Thr Ala Val Glu Arg Glu Thr Ala Ala Leu Ala Ala Gln Gly Leu Gly980 985 990Asn Arg Glu Ile Ala Thr Glu Leu Ala Val Thr Thr Arg Ala Val Glu995 10001005Leu Arg Leu Ser Gly Val Tyr Arg Lys Leu Arg Ile Arg Gly Arg101010151020Glu Glu Leu Arg Ala Leu Val Gln Glu Ala Glu Gly Ser102510301035<210>13<211>1005<212>PRT<213>鏈霉菌FR-008<400>13Met Trp Leu Arg Trp Ala Gly Asp Arg Ser Arg Gly Ala Ala Pro Gly1 5 10 15Arg Ser Gly Ser Ala Gly Pro Pro Ser Leu Ala Pro Ser Asp Ala Arg20 25 30Glu Ser Pro Val Pro His Arg Ser Arg Ala Val Pro Pro Ala Pro Pro35 40 45Leu Val Gly Arg Ala His Gln Leu Asp Val Leu Ala Arg His Ala Asp50 55 60
Ala Ala Arg Ala Gly Arg Pro Arg Leu Val Leu Leu Asp Gly Pro Ala65 70 75 80Gly Ile Gly Lys Thr Ala Leu Leu Arg Ala Ala Leu Ala Glu Asp Gly85 90 95Pro Leu Ala Gly Met Thr Thr Leu Tyr Gly Ser Cys Arg Ala Val Asp100 105 110Val Ala Thr Gly Tyr Ser Gly Val Arg Ala Leu Phe Gly Gly Leu Gly115 120 125Leu Thr Gly Arg Lys Gly Arg Thr Ser Pro Leu Leu Val Gly Gly Ala130 135 140Arg Arg Ala Leu Pro Ala Leu Ala Ala Asp Pro Gly Glu Leu Asp Ala145 150 155 160Asp Pro Gly Ser Thr Phe Ser Val Leu Gln Gly Leu Tyr Trp Leu Ala165 170 175Ala Asn Leu Met Ala Asp Gly Pro Leu Val Leu Val Leu Asp Asp Val180 185 190His Trp Cys Asp Glu Arg Ser Leu Arg Trp Leu Asp Phe Leu Leu Arg195 200 205Arg Ala Asp Gly Leu Pro Leu Leu Val Val Ala Ala His Arg Thr Gly210 215 220Thr Gly Leu Thr Ala Pro Asp Ala Leu Ala Asp Leu Val Ala His His225 230 235 240Leu Pro Ala Ser Leu Gly Leu Gly Pro Leu Asp Thr Ala Glu Val Ala245 250 255Glu Leu Thr Ala His Ser Phe Pro Glu Gln Asp Pro Leu Pro Ser Phe260 265 270Val Gly Arg Leu Leu Ser Val Thr Gly Gly Ser Pro Leu Glu Ile Val275 280 285Arg Leu Leu Arg Asp Leu Arg Ser Ala Gly Leu Gly Pro Asp Asp Thr290 295 300Gly Thr Ala Arg Leu Ala Gln Ala Gly Gly Lys Val Val Ala Ala Ser305 310 315 320Val Arg Gly Val Leu Glu His Gln Pro Asp Trp Val Arg Gln Val Ala325 330 335Arg Ala Val Ala Val Leu Gly Asp Glu Asp Arg Thr Tyr Leu Ala Ala340 345 350Leu Gly Arg Val Ser Val Val His Val Glu Glu Ala Val Glu Ile Leu355 360 365Arg Asp Ala Gly Leu Ile His Pro Gly His Leu Glu Leu Ser His Asp370 375 380Leu Val Arg Ala Ala Val Leu Asp Ala Val Gly Ala Ser Gly Val Ala385 390 395 400Val Leu Arg Arg Arg Ala Ala Arg Leu Leu Ser Asp Val Gly Arg Ser405 410 415
Pro Glu Glu Ile Ala Thr Gln Leu Leu Leu Val Pro Gly Thr Pro Asp420 425 430Asp Trp Ala Val Ser Val Leu Arg Asp Ala Ala Ala Gln Ala Glu Gln435 440 445Arg Gly Ala Cys Glu Ala Ala Ala Arg Tyr Leu Glu Arg Val Arg Glu450 455 460Ala Glu Pro Lys Asp Pro Asp Val Leu Ser Arg Leu Gly Lys Ala Leu465 470 475 480Ala Glu Thr Asp Pro Ala Arg Ser Val Thr Leu Leu His Glu Ala His485 490 495Ser Leu Thr Thr Asp Val Arg Ala Arg Ala Ala Thr Ala Val Gln Leu500 505 510Gly Leu Thr Cys Leu Ala Val Gln Gln Ser Pro Asp Gly Ala Arg Ala515 520 525Leu Thr Glu Ala Leu Asp Ala Leu Asp Ala Glu Leu Gly Pro Glu Pro530 535 540Glu Pro Ala Asp Arg Glu Leu Arg Thr Leu Thr Glu Ser Ala Leu Leu545 550 555560Ile Val Gly Ser Asp Glu Lys Ala Thr Leu Pro Asp Ile Leu Arg Arg565 570 575Thr Glu Gly Leu Thr Pro Gln Pro Gly Asp Thr Pro Ala Gln Arg Gln580 585 590Gln Leu Ala Met Leu Ser Val Leu Ser Ala Ala Glu Gly Gly Gly Ala595 600 605Glu Glu Thr Val Gly Arg Ala Arg Arg Ala Leu Arg Ala Pro Gly Val6l0 6l5 620Pro Leu Gly Val Trp Ser Leu Leu Pro Thr Ser Leu Ala Leu Ser Leu625 630 635 640Ala Asp Glu Asn Glu Ala Ala Glu Glu Val Leu Glu Thr Val Leu Arg645 650 655Gly Ser Gly Asp Thr Ala Ala Val Trp Thr Tyr Val Leu Ala Leu Ser660 665 670Thr Arg Ser Leu Phe Arg Leu Glu Asn Gly Ala Val Pro Asp Ala Met675 680 685Ala Asp Ala Gln Thr Ala Leu Glu Ile Leu Gly Gln Glu Arg Trp Gly690 695 700Asp Thr Ala Thr Met Pro His Thr Ala Tyr Ala Ser Val Leu Thr Glu705 710 715 720Arg Gly Asp Pro Gly Arg Ala Leu Glu Ala Leu Asp Ala Ile Lys Arg725 730 735Pro His Leu Asp Arg Phe Val Trp Glu Tyr His Trp Tyr Leu Met Ala740 745 750Arg Gly Arg Ala Leu Ala Ala Asp Gly Asp Leu Asp Gly Ala Leu Gln755 760 765
Val Phe Gly Ser Cys Gly Ala Ser Met Ala Glu Ala Gly Leu Thr Asn770 775 780Pro Val Leu Ala Pro Trp Trp Leu Glu Thr Ala Cys Leu Leu Gly Glu785 790 795 800Ala Gly Arg Gly Glu Glu Ala Gly Arg Ala Ala Ala His Gly Thr Arg805 810 815Leu Ala Glu Arg Trp Gly Thr Arg Arg Ala Leu Gly Tyr Ala Ala Leu820 825 830Ala Arg Gly Ala Ala Ala Arg Gly Thr Ala Arg Thr Gly Ala Leu Arg835 840 845Glu Ala Val Ala Leu Leu Ala Asp Ser Pro Ala Arg Ser Gly His Ala850 855 860Arg Gly Leu Leu Leu Leu Gly Arg Ala Leu Val Glu Asp Gly Ala Val865 870 875 880Arg Glu Gly Arg Glu His Leu Arg Glu Ala Val Gly Leu Ala Arg Arg885 890 895Cys Gly Cys Val Ala Leu Ala Arg Arg Ala Arg Asp Glu Leu Ile Ala900 905 910Ala Gly Gly Arg Met Arg Glu Val Thr Ala Ser Pro Leu Asp Met Leu915 920 925Thr Gly Thr Glu Arg Thr Val Ala Gly Leu Val Ala Ser Gly Ala Gly930 935 940Asn Arg Glu Val Ala Glu Ser Leu Phe Val Thr Val Arg Thr Val Glu945 950 955 960Leu His Leu Thr Ser Val Tyr Arg Lys Leu Gly Val Ala Arg Arg Gly965 970 975Asp Leu Thr Glu Ala Leu Arg Glu Ala Gly Ala Thr Ala Arg Pro Ala980 985 990Ala Glu Arg Arg Pro Gly His Ala Lys Arg Arg Asn Pro995 10001005<210>14<211>393<212>PRT<213>鏈霉菌FR-008<400>14Met Thr Thr Ser Pro Gly Pro Thr Val Val Asp Phe Pro Arg Arg Thr1 5 10 15Pro Arg Glu Pro Leu Pro Leu Ser Gln Tyr Ala Glu His Arg Lys Gln20 25 30Asn Gly Leu Val Gln Thr His Leu Pro Asn Gly Arg Pro Ile Trp Leu35 40 45
Val Thr Arg His Glu Asp Val Arg Ala Val Leu Thr His Pro Arg Ile50 55 60Ser Ala Asn Pro Asp Asn Glu Gly Phe Pro Asn Val Gly Glu Thr Met65 70 75 80Gly Val Pro Lys Gln Glu Gln Ile Pro Gly Trp Phe Val Gly Leu Asp85 90 95Ser Pro Glu His Asp Arg Phe Arg Lys Val Leu Ile Pro Glu Phe Thr100 105 110Val Arg Arg Val Arg Glu Leu Arg Pro Ala Ile Glu Arg Thr Val Asp115 120 125Glu Arg Ile Asp Ala Met Leu Ala Gly Gly Asn Thr Ala Asp Leu Val130 135 140Asn Asp Phe Ala Leu Pro Val Pro Ser Leu Val Ile Ser Ala Leu Leu145 150 155 160Gly Val Pro Ser Ala Asp Arg Asp Phe Phe Glu Ser Arg Thr Arg Thr165 170 175Leu Val Ala Ile Arg Thr Ser Thr Asp Glu Glu Arg Ala Glu Ala Thr180 185 190Arg Gln Leu Leu Arg Tyr Ile Asn Arg Leu Ile Val Ile Lys Lys Lys195 200 205Trp Arg Gly Glu Asp Leu Ile Ser Arg Leu Leu Ser Thr Gly Lys Leu210 215 220Ser Asp Glu Glu Leu Ser Gly Val Leu Leu Leu Leu Leu Ile Ala Gly225 230 235 240His Glu Thr Thr Ala Asn Asn Ile Gly Leu Gly Val Val Thr Leu Leu245 250 255Ser His Arg Glu Trp Ile Gly Asp Asp Arg Leu Val Glu Glu Leu Leu260 265 270Arg Leu His Ser Val Ala Asp Met Val Ala Leu Arg Val Ala Val Asp275 280 285Asp Val Glu Ile Ala Gly Gln Thr Ile Arg Lys Gly Glu Gly Ile Val290 295 300Pro Leu Leu Ala Ser Ala Asn His Asp Thr Glu Ala Phe Gly Cys Pro305 310 315 320His Ala Phe Asn Pro Glu Arg Thr Glu Arg Arg His Val Ala Phe Gly325 330 335Tyr Gly Val His Gln Cys Leu Gly Gln Asn Leu Val Arg Val Glu Met340 345 350Glu Ile Ala Tyr Arg Lys Leu Phe Glu Arg Ile Pro Glu Leu Arg Leu355 360 365Ala Val Pro Glu Asp Gln Leu Ala Tyr Lys Tyr Asp Gly Ile Leu Phe370 375 380Gly Leu His Glu Leu Pro Val Arg Trp385 390
<210>15<211>64<212>PRT<213>鏈霉菌FR-008<400>15Met Arg Val Thr Val Asp Ser Glu Gln Cys Val Gly Ala Gly Gln Cys1 5 10 15Val Leu Asn Ala Pro Glu Val Phe Asp Gln Asp Asp Asp Gly Val Val20 25 30Val Leu Leu Arg Ala Glu Pro Asp Glu Arg Asp His Glu Ala Val Arg35 40 45Thr Ala Gly Asp Leu Cys Pro Ser Ala Ser Val Val Leu Gln Glu Asp50 55 60<210>16<211>285<212>PRT<213>鏈霉菌FR-008<400>16Met Pro Arg Pro Ala Arg Pro Ser His Pro Arg Thr Arg Arg Arg Arg1 5 10 15Asn Thr Asp Arg Arg Gln Asn His Arg Arg Asp Ser Pro Val Thr Thr20 25 30Ala Asp Asp Thr Ala Ala Arg Trp Leu Arg Arg Tyr His Pro Ala Glu35 40 45Ala Asp Ala Val Arg Leu Val Cys Phe Pro His Ala Gly Gly Ser Ala50 55 60Ser Phe Tyr His Pro Val Ser Ala Arg Phe Ala Pro Gly Ala Glu Val65 70 75 80Val Ser Leu Gln Tyr Pro Gly Arg Gln Asp Arg Arg Lys Glu Pro Cys85 90 95Val Pro Asp Leu Gly Thr Leu Ala Asp Leu Ile Thr Glu Gln Leu Leu100 105 110Pro Leu Asp Glu Arg Pro Thr Val Phe Phe Gly His Ser Met Gly Ala115 120 125Ala Leu Ala Phe Glu Thr Ala Trp Arg Leu Glu Gln Lys Gly Ala Gly130 135 140Pro Arg Thr Val Ile Ala Ser Gly Arg Arg Gly Pro Ser Thr Thr Arg
145 150 155 160Ala Glu Arg Val His Thr Arg Asp Asp Asp Gly Ile Val Ala Glu Met165 170 175Lys Arg Leu Asn Gly Thr Ala Ala Gly Val Leu Gly Asp Glu Glu Ile180 185 190Leu Arg Met Ala Leu Pro Ala Leu Arg Gly Asp Tyr Arg Ala Ile Glu195 200 205Thr Tyr Thr Cys Pro Pro Asp Arg Arg Leu Ala Cys Gly Leu Thr Val210 215 220Leu Thr Gly Glu Asp Asp Pro Leu Thr Thr Val Glu Glu Ala Glu Arg225 230 235 240Trp Arg Asp His Thr Thr Gly Pro Phe Arg Leu Arg Val Phe Thr Gly245 250 255Gly His Phe Phe Leu Thr Gln His Leu Asp Ala Val Asn Thr Glu Ile260 265 270Ala Gln Ala Leu His Pro Asp Arg Ala Ala Pro Ala Ala275 280 285<210>17<211>458<212>PRT<213>鏈霉菌FR-008<400>17Met Asp Ser Ala Ala Arg Pro Ile Leu Phe Val Ser Leu Pro Glu Ser1 5 10 15Gly Leu Leu Asn Pro Leu Leu Val Leu Ala Gly Glu Leu Ala Arg Arg20 25 30Gly Val Pro Asp Leu Trp Phe Ala Thr Asp Glu His Arg Arg Glu Glu35 40 45Val Glu Ala Leu Ser Asp Val Ser Lys Val Ser Phe Ala Ser Leu Gly50 55 60Glu Val Val Pro Glu Leu Ser Ala Val Thr Trp Asp Asp Glu Val Tyr65 70 75 80Arg Glu Val Thr Gln Ser Ser Arg Phe Lys Ala His Arg Ala Val Val85 90 95Arg Gln Ser Tyr Arg Pro Ala Leu Gln Ala Arg Lys Tyr Arg Glu Leu100 105 110Glu Gln Val Val Asp Glu Val Arg Pro Ala Leu Met Val Val Asp Cys115 120 125Val Ala Gly Phe Gly Val Asp Leu Ala Leu Ala Arg Gly Ile Pro Tyr130 135 140
Val Leu Asn Val Pro Phe Val Ala Ser Asn Val Leu Thr Ser His Asn145 150 155 160Pro Phe Gly Ala Ser Tyr Thr Pro Lys Ser Phe Pro Val Pro Asn Ser165 170 175Gly Leu Pro Ala Arg Met Ser Val Arg Gln Lys Leu Ala Asn Thr Leu180 185 190Phe Lys Trp Arg Thr Leu Gly Met Phe Leu His Pro Asp Met Ala Ala195 200 205Leu Leu Arg Glu Asp Ala Ala Ile Arg Lys Glu Leu Gly Ile Ala Pro210 215 220Pro Asn Ala Met Thr Arg Val Asp Glu Ala Ala Ala Val Val Cys Ser225 230 235 240Ser Val Ala Glu Leu Asp Tyr Pro Phe Asp Ile Pro Asp Arg Val Ser245 250 255Leu Val Gly Ala Val Leu Pro Pro Leu Pro Glu Ala Pro Asp Asp Asp260 265 270Glu Val Thr Arg Trp Leu Asp Ala Gln Ser Ser Val Val Tyr Met Gly275 280 285Phe Gly Thr Ile Thr Arg Leu Thr Arg Glu Glu Val Ala Ala Leu Val290 295 300Glu Val Ala Arg Arg Met Ser Gly Thr His Gln Phe Leu Trp Lys Leu305 310 315 320Pro Lys Glu Gln Gln His Leu Leu Pro Glu Ala Gly Ser Leu Pro Asp325 330 335Asn Leu Arg Val Glu Ser Trp Val Pro Ser Gln Leu Asp Val Leu Ala340 345 350His Pro Asn Val Ser Val Phe Phe Ser His Gly Gly Gly Asn Ala Tyr355 360 365His Glu Gly Val Tyr Phe Gly Lys Pro Gln Val Val Arg Pro Leu Trp370 375 380Val Asp Cys Phe Asp Gln Ala Val Arg Gly Arg Asp Phe Gly Ile Ser385 390 395 400Leu Thr Leu Asp Lys Pro His Thr Val Asp Pro Asp Asp Val Val Asp405 410 415Lys Leu Thr Arg Val Thr Ser Asp Pro Ala Phe Arg Thr Glu Ala Glu420 425 430Arg Leu Gly Ala Leu Gln Arg Ala Ala Gly Gly Arg Ala Ala Ala Ala435 440 445Asp Leu Val Thr Gly Leu Leu Pro Ala Ala450 455<210>18
<211>352<212>PRT<213>鏈霉菌FR-008<400>18Met Ala Phe Thr His Pro Val Ser Arg Pro Ala Leu Asp Gly Arg Glu1 5 10 15Leu Glu Tyr Val Ser Asp Ala Val Ser Gly Gly Trp Ile Ser Ser Gln20 25 30Gly Pro Tyr Val Arg Arg Phe Glu Glu Ala Phe Ala Glu Trp Asn Gly35 40 45Val Ala His Gly Val Ala Cys Ser Ser Gly Thr Ala Ala Leu Thr Leu50 55 60Ala Leu Arg Ala Leu Asn Ile Gly Pro Gly Asp Glu Val Ile Val Pro65 70 75 80Glu Phe Thr Met Val Ala Ser Ala Trp Ala Val Thr Tyr Thr Gly Ala85 90 95Thr Pro Val Phe Val Asp Cys Gly Asp Asp Leu Asn Ile Asp Val Thr100 105 110Arg Ile Glu Glu Lys Ile Thr Pro Arg Thr Arg Ala Val Met Pro Val115 120 125His Val Tyr Gly Arg Arg Cys Asp Met Asp Ala Val Met Asp Leu Ala130 135 140Leu Gln Tyr Asn Leu Arg Val Val Glu Asp Ser Ala Glu Ala His Gly145 150 155 160Val Arg Pro Val Gly Asp Ile Ala Cys Phe Ser Leu Phe Ala Asn Lys165 170 175Ile Ile Thr Ala Gly Glu Gly Gly Val Cys Leu Thr Asp Asp Pro Arg180 185 190Leu Ala Glu Gln Leu Ala His Leu Arg Ala Met Ala Phe Thr Arg Asp195 200 205His Ser Phe Leu His Lys Lys Leu Ala Tyr Asn Tyr Arg Met Thr Ala210 215 220Met Gln Gly Ala Val Ala Leu Ala Gln Thr Glu Arg Leu Asp Glu Ile225 230 235 240Leu Ala Thr Arg Arg Glu Ile Glu Ala Arg Tyr Asp Ala Gly Leu Lys245 250 255Asp Leu Pro Gly Ile Thr Leu Met Pro Ala Arg Asp Val Leu Trp Met260 265 270Tyr Asp Leu Arg Ala Glu Arg Arg Glu Glu Leu Arg Ala His Leu Asp275 280 285Ala Arg Gly Ile Glu Thr Arg Leu Phe Phe Lys Pro Met Ser Arg Gln290 295 300Pro Gly Tyr Leu Asp Pro Val Trp Pro Thr Leu Asn Ala His Arg Phe
305 310 315 320Ser Glu Asp Gly Leu Tyr Leu Pro Thr His Thr Gly Leu Thr Ala Ala325 330 335Asp Gln Glu Tyr Ile Thr Gly Ala Val Arg Asp Phe Tyr Arg Ala Gly340 345 350<210>19<211>402<212>PRT<213>鏈霉菌FR-008<400>19Met Ser Ala Thr Arg Thr Ala Arg Glu Arg Ser Arg Ala Ser Arg Ala1 5 10 15Pro Glu Gly Gly Lys Ala Leu Pro Ser Ala Gly Ala Cys Gln Tyr Ala20 25 30Trp Gly Ser Ile Ser Pro Pro Pro Pro Ala Arg Thr Pro Ala Arg Pro35 40 45Val Leu Thr Val Ala Val Glu Arg Gly Lys Met Ser Lys Arg Ala Leu50 55 60Ile Thr Gly Ile Thr Gly Gln Asp Gly Ser Tyr Leu Ala Glu His Leu65 70 75 80Leu Asp Gln Gly Tyr Gln Val Trp Gly Leu Cys Arg Gly Gln Ala Asn85 90 95Pro Arg Lys Asp Arg Ile Ala Lys Leu Ile Pro Glu Leu Ser Phe Val100 105 110Asp Gly Asp Leu Met Asp Gln Gly Ser Leu Val Ser Ala Val Asp Leu115 120 125Val Gln Pro Asp Glu Val Tyr Asn Leu Gly Ala Ile Ser Phe Val Pro130 135 140Met Ser Trp Gln Gln Pro Glu Leu Val Thr Glu Val Asn Gly Thr Gly145 150 155 160Val Leu Arg Met Leu Glu Ala Val Arg Ile Val Ser Gly Leu Thr Lys165 170 175Ser Ser Gly Gly Ser Pro Arg Gly Gln Ile Arg Phe Tyr Gln Ala Ser180 185 190Ser Ser Glu Met Tyr Gly Lys Val Ala Glu Ser Pro Gln Arg Glu Thr195 200 205Thr Ser Phe His Pro Arg Ser Pro Tyr Gly Val Ala Lys Ala Phe Gly210 215 220His Tyr Ile Thr Gln Asn Tyr Arg Glu Ser Tyr Gly Met Tyr Gly Val225 230 235 240Ser Gly Ile Leu Phe Asn His Glu Ser Pro Arg Arg Gly Ala Glu Phe
245 250 255Val Thr Arg Lys Ile Ser Leu Ala Val Ala Gln Ile Lys Leu Gly Gln260 265 270Met Asp Lys Leu His Leu Gly Asn Leu Asp Ala Glu Arg Asp Trp Gly275 280 285Phe Ala Gly Asp Tyr Val Arg Ala Met His Leu Met Leu Gln Gln Glu290 295 300Gln Ala Gly Asp Tyr Val Val Gly Thr Gly Ala Met His Gln Val Arg305 310 315 320Asp Ala Ala Arg Ile Ala Phe Glu His Val Gly Leu Asp Trp Gln Glu325 330 335His Val Val Val Asp Pro Gly Leu Val Arg Pro Ala Glu Val Glu Thr340 345 350Leu Cys Ala Asp Ser Gly Asn Ala Arg Arg Glu Leu Gly Trp Glu Pro355 360 365Glu Val Asp Phe Glu Gln Leu Met Arg Met Met Val Glu Ser Asp Leu370 375 380Arg Gln Ala Ser Arg Glu Arg Asp Tyr Ser Gln Leu Leu Ala Thr Gly385 390 395 400Ser Trp<210>20<211>458<212>PRT<213>鏈霉菌FR-008<400>20Met Pro Ser Thr Cys Pro Ala Pro Thr Ser Ile Gly Phe Arg Gly Cys1 5 10 15Pro His Arg Arg Lys Gly Arg Thr Ser Arg Gly Arg Arg Ile Ala Val20 25 30Pro Thr Arg Pro Cys Gly Lys Val Arg Lys Gly His Gln His Ala Lys35 40 45Lys Trp Arg Val Ser Met Arg Gly Gly Ser Val Ala Val Val Gly Gly50 55 60Ser Ile Ala Gly Cys Ala Ala Ala Leu Ala Ala Ser Arg Gly Gly Ala65 70 75 80Glu Arg Ile Thr Val Phe Glu Arg Ala Asp Ala Arg Leu Arg Asp Arg85 90 95Gly Val Gly Ile Gly Val His Asp Ala Arg Tyr Ala Glu Leu Arg Asp100 105 110Ala Gly Tyr Met Ala Asp Glu Met Pro Trp Ala Pro Leu Asn Arg Arg115 120 125
Val Trp Ser Val Arg Asp Gly Asp Ala Glu His Gly Arg Ala Ile Gly130 135 140Thr Gln Pro Phe Pro Phe Arg Ala Tyr Asn Trp Gly Ser Leu Trp Ser145 150 155 160Glu Leu Arg Arg Arg Val Pro Glu Thr Ala Thr Tyr Arg Ala Gly Ala165 170 175Lys Val Glu Ala Val Glu Gln Asp Ala Asp Gly Val Thr Val Arg Leu180 185 190Ala Asp Gly Glu Pro Glu Arg Phe Asp Leu Val Ile Gly Ala Asp Gly195 200 205Tyr Arg Ser Val Val Arg Glu Ala Met Tyr Pro Gly Thr Gly Ala Ala210 215 220Tyr Ala Gly Tyr Ile Gly Trp Arg Gly Thr Ser Pro Asp Val Ser Gly225 230 235 240Leu Pro Ser Asp Gly Leu Asp Ala His Asn Ile Thr Phe Pro Gly Gly245 250 255His Cys Met Ala Tyr Arg Ile Pro Asp Gly Ser Gly Gly His Arg Leu260 265 270Asn Trp Val Leu Tyr Thr Ala Pro Pro Arg Ile Asp Gly Leu His Pro275 280 285Asp Leu Arg Thr Pro Thr Ser Leu Pro Pro Gly Arg Leu Asn Ala Glu290 295 300Leu Thr Glu His Leu Arg Ala Leu Val Ala Glu His Phe Pro Pro Phe305 310 315 320Trp Ala Ala Lys Leu Leu Ser Thr Pro Ala Glu Thr Thr Phe Ile Gln325 330 335Pro Ile Tyr Asp Leu Asp Val Pro His Tyr Ala Thr Asp Arg Met Val340 345 350Leu Ile Gly Asp Ala Ala Ser Val Ala Arg Pro His Leu Gly Ala Gly355 360 365Ser Val Lys Ala Leu Gln Asp Ala Thr Ala Leu Glu Ala Ala Trp Val370 375 380Ala Gly Glu Ser Trp Lys Glu Ile Leu Glu Gly Tyr His Ala Ala Arg385 390 395 400Gly Pro Val Gly Thr Ala Met Val Gly Leu Ala Arg Arg Met Gly Ser405 410 415Ala Gln Val Glu Glu Thr Pro Asp Trp Ser Ala Met Gly Gln Ala Glu420 425 430Phe Asp Ala Trp Trp Gln Glu Gln Asn Asn Gly Ser Asp Arg Arg Ser435 440 445Gly Phe Gly Gly His Ser Leu Lys Ser Arg450 455
<210>21<211>723<212>PRT<213>鏈霉菌FR-008<400>21Met Arg Thr Leu Leu Val Asp Asn Tyr Asp Ser Phe Thr Tyr Asn Leu1 5 10 15Phe His Tyr Leu Ser Arg Ala Asn Gly Arg Glu Pro Glu Val Ile Arg20 25 30Asn Asp Asp Pro Ala Trp Arg Pro Gly Leu Leu Asp Ala Phe Asp Asn35 40 45Val Val Leu Ser Pro Gly Pro Gly Thr Pro His Arg Pro Ala Asp Phe50 55 60Gly Leu Cys Ala Arg Ile Ala Glu Glu Gly Arg Leu Pro Val Leu Gly65 70 75 80Val Cys Leu Gly His Gln Gly Met Ala Leu Ala His Gly Ala Arg Val85 90 95Gly Arg Ala Pro Glu Pro Arg His Gly Arg Thr Ser Ala Val Arg His100 105 110Asp Gly Thr Gly Leu Phe Glu Gly Leu Pro Gln Pro Leu Glu Val Val115 120 125Arg Tyr His Ser Leu Ala Val Thr Glu Leu Pro Pro Glu Leu Glu Ala130 135 140Thr Ala Trp Ser Glu Asp Gly Val Leu Met Ala Leu Arg His Arg Thr145 150 155 160Leu Pro Leu Trp Gly Val Gln Phe His Pro Glu Ser Ile Gly Thr Gln165 170 175Asp Gly His Arg Leu Leu Ala Asn Phe Arg Asp Leu Thr Glu Arg His180 185 190Gly Arg Thr Arg Pro Gly Gly Arg Ala Gly His Gly Thr Leu Pro Pro195 200 205Pro Ala Pro Ala Arg Glu Thr Thr Ala Thr Thr Gly Thr Pro Arg Arg210 215 220Leu Arg Val Ile Ala Glu Ser Leu Pro Thr Arg Trp Asp Ala Glu Val225 230 235 240Ala Phe Asp Ser Leu Phe Arg Thr Gly Asp His Pro Phe Trp Leu Asp245 250 255Ser Ser Arg Pro Gly Gly Glu Leu Gly Gln Leu Ser Val Met Gly Asp260 265 270Ala Ser Gly Pro Leu Ala Arg Thr Ala Lys Ala Asp Val His Ala Gly275 280 285Thr Val Thr Val Arg Ala Asp Gly Ala Ser Ser Thr Val Glu Ser Ala290 295 300
Phe Leu Thr Trp Leu Glu Asn Asp Leu Ala Gly Leu Arg Thr Glu Val305 310 315 320Pro Glu Leu Pro Phe Ala Phe Ala Leu Gly Trp Val Gly Cys Leu Gly325 330 335Tyr Glu Leu Lys Ala Glu Cys Asp Gly Asp Ala Ala His Arg Ser Pro340 345 350Asp Pro Asp Ala Val Leu Val Phe Ala Asp Arg Ala Leu Val Leu Asp355 360 365His Arg Thr Arg Thr Thr Tyr Leu Leu Ala Leu Val Glu Asp Asp Ala370 375 380Glu Ala Glu Ala Arg Ala Trp Leu Ala Ala Ala Ser Ala Thr Leu Glu385 390 395 400Ala Ile Ala Gly Arg Glu Pro Glu Pro Cys Pro Glu Ala Pro Val Cys405 410 415Thr Thr Gly Pro Val Glu Leu Arg His Asp Arg Asp Gly Tyr Leu Lys420 425 430Leu Ile Asp Val Cys Gln Gln Glu Ile Ala Ala Gly Glu Thr Tyr Glu435 440 445Val Cys Leu Thr Asn Met Ala Glu Ala Asp Thr Asp Leu Thr Pro Trp450 455 460Ala Ala Tyr Arg Ala Leu Arg Arg Val Ser Pro Ala Pro Phe Ala Ala465 470 475 480Phe Leu Asp Phe Gly Pro Met Ala Val Leu Ser Ser Ser Pro Glu Arg485 490 495Phe Leu Arg Ile Asp Arg His Gly Arg Met Glu Ser Lys Pro Ile Lys500 505 510Gly Thr Arg Pro Arg Gly Ala Thr Pro Gln Glu Asp Ala Ala Leu Val515 520 525Arg Ala Leu Ala Thr Cys Glu Lys Asp Arg Ala Glu Asn Leu Met Ile530 535 540Val Asp Leu Val Arg His Asp Leu Gly Arg Cys Ala Glu Val Gly Ser545 550 555 560Val Val Ala Asp Pro Val Phe Gln Val Glu Thr Tyr Ala Thr Val His565 570 575Gln Leu Val Ser Thr Val Thr Ala Arg Leu Arg Glu Asp Ser Ser Pro580 585 590Val Ala Ala Val Arg Ala Ala Phe Pro Gly Gly Ser Met Thr Gly Ala595 600 605Pro Lys Ile Arg Thr Met Gln Ile Ile Asp Arg Leu Glu Gly Gly Pro610 615 620Arg Gly Val Tyr Ser Gly Ala Ile Gly Tyr Phe Ser Leu Thr Gly Ala625 630 635 640Val Asp Leu Ser Ile Val Ile Arg Thr Val Val Leu Ser Gly Gly Arg645 650 655
Leu Arg Tyr Gly Val Gly Gly Ala Val Ile Ala Leu Ser Asp Pro Ala660 665 670Asp Glu Phe Glu Glu Thr Ala Val Lys Ala Ala Pro Leu Leu Arg Leu675 680 685Leu Asp Thr Ala Phe Pro Gly Arg Glu Ala Pro Gly Lys Asp Leu Asp690 695 700Gly Glu Pro Asp Asp Gly Thr Asp Ala Gly Ala Pro Lys Asp Leu Val705 710 715 720Leu Pro Gly<210>22<211>257<212>PRT<213>鏈霉菌FR-008<400>22Met Ile Glu Leu Asp Gly Glu Pro Ala Gly Pro Glu Ala Leu Ala Ser1 5 10 15Leu Ala Leu Thr Asn Tyr Gly His Phe Thr Thr Leu Leu Val Glu Asn20 25 30Gly Arg Val Arg Gly Leu Asp Leu His Leu Glu Arg Leu Ile Arg Asp35 40 45Cys Arg Thr Leu Phe Asp Ala Ala Leu Asp Pro Asp Arg Val Arg Lys50 55 60Leu Ala Arg Arg Ala Ala Pro Thr Asp Gly Arg Ala Thr Val Arg Val65 70 75 80Thr Val Phe Asp Pro Ala Leu Asn Leu Gly Asn Ile Ala Ala Asp Ala85 90 95Arg Pro Gly Ile Leu Val Thr Ser Arg Pro Ala Pro Asp Lys Pro Pro100 105 110Gly Pro Leu Arg Val Arg Ser Val Val His Arg Arg Asp Leu Pro Glu115 120 125Val Lys Ser Val Gly Leu Cys Pro Thr Leu Arg Leu Arg Arg Gln Ala130 135 140Gln Arg Ala Gly Tyr Asp Asp Val Leu Phe Thr Gly Pro Asp Gly Asp145 150 155 160Ile Leu Glu Gly Gly Thr Trp Asn Val Gly Leu Val Arg Asp Gly Glu165 170 175Val Val Trp Pro Gly Gly Glu Val Leu Ala Gly Thr Thr Arg Gln Leu180 185 190Leu Arg Arg Gly His Arg Arg Ser His Arg Thr Gly Arg Pro Arg Gly195 200 205
Pro Arg Leu Ser Arg Gly Gly Leu Arg Asp Gln Arg Arg Val Gly Val210 215 220Arg Pro Val Thr Gly Ile Asp Asp Arg Glu Phe Pro Ala Ala His His225 230 235 240Ser Val Thr Arg Leu Ala Glu Ile Tyr Gln Ala Leu Pro Gly Ser Pro245 250 255Leu
權(quán)利要求
1.一種鏈霉菌FR-008中多烯聚酮FR-008生物合成基因簇，其特征在于，包含fscA，fscB，fscC，fscD，fscE，fscF，fscTI，fscTII，fscRI，fscRII，fscRIII，fscRIV，fscP，fscFE，fscTE，fscMI，fscMII，fscMIII，fscO，pabAB，pabC這一系列共21個(gè)基因及其DNA序列，即序列1，并提供了這些基因及其DNA序列所編碼FR-008生物合成酶的氨基酸序列，即序列2-22。
2.根據(jù)權(quán)利要求1所述的鏈霉菌FR-008中多烯聚酮FR-008生物合成基因簇，其特征是，包括包含序列1中堿基20,927-26,158的基因fscA，包含序列1中堿基60,860-28,983的基因fscC，包含序列1中堿基77,587-60,962的基因fscB，包含序列1中堿基84,132-77,983的基因fscF，包含序列1中堿基107,485-84,170的基因fscE，包含序列1中堿基136,148-107,496的基因fscD的核苷酸序列，編碼了聚酮合酶。
3.根據(jù)權(quán)利要求1所述的鏈霉菌FR-008中多烯聚酮FR-008生物合成基因簇，其特征是，包括包含序列1中堿基26,333-27,340的基因fscTI，包含序列1中堿基27,561-28,280的基因fscTII，包含序列1中堿基3,818-3,150的基因fscRI，包含序列1中堿基7,205-4,377的基因fscRII，包含序列1中堿基10,320-7,210的基因fscRIII，包含序列1中堿基13,315-10,298的基因fscRIV，包含序列1中堿基16,106-17,287的基因fscP，包含序列1中堿基17,334-17,528的基因fscFE，包含序列1中堿基17,556-18,413的基因fscTE，包含序列1中堿基13,522-14,898的基因fscMI，包含序列1中堿基14,953-16,011的基因fscMII，包含序列1中堿基137,766-136,558的基因fscMIII，包含序列1中堿基1,950-574的基因fscO，包含序列1中堿基18,610-20,781的基因pabAB，包含序列1中堿基3,037-2,264的基因pabC的核苷酸序列，編碼了ABC轉(zhuǎn)運(yùn)蛋白，調(diào)節(jié)蛋白，細(xì)胞色素P450單氧化酶，鐵氧化還原蛋白，II型硫脂酶，糖基合成酶，糖苷轉(zhuǎn)移酶，依賴FAD的單氧化酶，對(duì)氨基苯甲酸合成酶。
4.根據(jù)權(quán)利要求1所述的鏈霉菌FR-008中多烯聚酮FR-008生物合成基因簇，其特征是，包括包含序列1中堿基20,927-26,158的基因fscA，包含序列1中堿基60,860-28,983的基因fscC，包含序列1中堿基77,587-60,962的基因facB，包含序列1中堿基84,132-77,983的基因fscF，包含序列1中堿基107,485-84,170的基因fscE，包含序列1中堿基136,148-107,496的基因fscD的聚酮合酶基因編碼了包含有聚酮合酶模塊的酮基合成酶結(jié)構(gòu)域即KS結(jié)構(gòu)域，?；D(zhuǎn)移酶結(jié)構(gòu)域即AT結(jié)構(gòu)域，?；d體蛋白結(jié)構(gòu)域即ACP結(jié)構(gòu)域，也可能包含酮基還原酶結(jié)構(gòu)域即KR結(jié)構(gòu)域，脫氫酶結(jié)構(gòu)域即DH結(jié)構(gòu)域，烯酰還原酶結(jié)構(gòu)域即ER結(jié)構(gòu)域的聚酮合酶。
5.根據(jù)權(quán)利要求1所述的鏈霉菌FR-008中多烯聚酮FR-008生物合成基因簇，其特征是，由序列2-22定義的FR-008生物合成酶對(duì)應(yīng)于FR-008生物合成的每一個(gè)合成步驟和后修飾過程以及調(diào)節(jié)，轉(zhuǎn)運(yùn)，矯正過程。
全文摘要
一種鏈霉菌FR－008中負(fù)責(zé)FR－008聚酮合成的基因簇，屬于基因技術(shù)領(lǐng)域。包含fscA，fscB，fscC，fscD，fscE，fscF，fscTI，fscTII，fscRI，fscRII，fscRIII，fscRIV，fscP，fscFE，fscTE，fscMI，fscMII，fscMIII，fscO，pabAB，pabC這一系列共21個(gè)基因及其DNA序列，即序列1，并提供了這些基因及其DNA序列所編碼FR－008生物合成酶的氨基酸序列，即序列2－22。本發(fā)明所提供的基因及其所編碼的蛋白質(zhì)可以用來查找和發(fā)展可用于醫(yī)藥，工業(yè)，農(nóng)業(yè)的化合物或蛋白。
文檔編號(hào)C07H21/04GK1667123SQ200310108110
公開日2005年9月14日 申請(qǐng)日期2003年10月23日 優(yōu)先權(quán)日2003年10月23日
發(fā)明者鄧子新, 陳實(shí), 周秀芬, 李商玉, 鄭克俊 申請(qǐng)人:上海交通大學(xué), 韓國(guó)高等科學(xué)技術(shù)學(xué)院