專利名稱：日本血吸蟲表皮抗原基因、蛋白及應(yīng)用的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及生物基因領(lǐng)域，具體地涉及一類日本血吸蟲表皮抗原基因、其編碼蛋白，還涉及該日本血吸蟲表皮抗原蛋白的應(yīng)用。
背景技術(shù)：
血吸蟲病由血吸蟲的感染引起，在世界上有76個(gè)國家，受感染者約1.5億。致病的血吸蟲有3種，既日本血吸蟲(Schistosoma japonicum)，曼氏血吸蟲(S.mansoni)和埃及血吸蟲(S.haematobium)。在我國流行的是日本血吸蟲。血吸蟲病的流行給人民身體健康和經(jīng)濟(jì)發(fā)展都造成巨大的損失，因此，防治血吸蟲病、開發(fā)研制治療血吸蟲病和抗血吸蟲的藥物具有巨大的社會(huì)和經(jīng)濟(jì)效益。
研制新的治療血吸蟲病和抗血吸蟲的藥物，首先必須找到合適的藥物作用靶點(diǎn)。血吸蟲的表皮是宿主和血吸蟲相互作用的界面。而組成血吸蟲表皮的蛋白質(zhì)暴露在這個(gè)界面上，對(duì)小分子的化學(xué)藥物和大分子的抗體藥物而言都是最理想的藥物作用靶點(diǎn)。已經(jīng)有研究表明治療血吸蟲的化學(xué)藥物——吡喹酮的作用靶點(diǎn)就是存在于血吸蟲的表皮。另外一些已知的血吸蟲表皮抗原蛋白，例如GST28，paramyosin，已經(jīng)用于疫苗研究和應(yīng)用。我們通過大規(guī)模的蛋白質(zhì)組學(xué)研究，發(fā)現(xiàn)了50個(gè)日本血吸蟲表皮抗原蛋白質(zhì)，這些蛋白質(zhì)，都是可能的藥物作用靶點(diǎn)和候選疫苗。
鑒于日本血吸蟲表皮抗原蛋白的重要功能，研究和開發(fā)日本血吸蟲表皮抗原蛋白具有重要意義。

發(fā)明內(nèi)容
本發(fā)明要解決的技術(shù)問題之一是提供一類日本血吸蟲表皮抗原基因。
本發(fā)明要解決的技術(shù)問題之二是提供一類日本血吸蟲表皮抗原蛋白。
本發(fā)明要解決的技術(shù)問題之三是提供上述日本血吸蟲表皮抗原蛋白的應(yīng)用。
為了解決上述技術(shù)問題，本發(fā)明通過如下技術(shù)方案實(shí)現(xiàn)在本發(fā)明一個(gè)方面，提供了一類日本血吸蟲表皮抗原基因，其編碼具有日本血吸蟲表皮抗原蛋白質(zhì)活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.25所示的序列；
(b)SEQ ID No.1～SEQ ID No.25所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.25所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數(shù)條的組合。
較佳地，所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.25所示的序列。
在本發(fā)明的另一方面，提供了上述日本血吸蟲表皮抗原蛋白，包括(a)具有SEQ ID No.26～SEQ ID No.50所示氨基酸序列的蛋白；(b)由(a)的蛋白發(fā)生一個(gè)或幾個(gè)氨基酸置換、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
較佳地，其具有SEQ ID No.26～SEQ ID No.50所示的氨基酸序列。
在本發(fā)明的另一方面，提供了一類能與上述日本血吸蟲表皮抗原蛋白特異性結(jié)合的抗體。在本發(fā)明中，可以使用一系列本領(lǐng)域已知的方法來制備針對(duì)日本血吸蟲表皮抗原蛋白的抗體。例如，將提純的日本血吸蟲表皮抗原蛋白產(chǎn)物或它的抗原片段注射入動(dòng)物體內(nèi)以產(chǎn)生多克隆抗體。同樣，表達(dá)日本血吸蟲表皮抗原蛋白或它的抗原片段的細(xì)胞也可以用來對(duì)動(dòng)物致免疫而產(chǎn)生抗體。本發(fā)明制備的抗體也可以是單克隆抗體，這些單克隆抗體可用雜交瘤技術(shù)制備。
在本發(fā)明的另一方面，提供了上述日本血吸蟲表皮抗原蛋白作為免疫原，在制備血吸蟲疫苗中的應(yīng)用。
在本發(fā)明的另一方面，提供了上述日本血吸蟲表皮抗原蛋白在制備防治血吸蟲病的藥物中的應(yīng)用。應(yīng)用本發(fā)明的日本血吸蟲表皮抗原蛋白，可研究血吸蟲治療藥物的作用靶點(diǎn)，從而在開發(fā)研制預(yù)防或治療血吸蟲病的藥物的過程中發(fā)揮重要作用。


圖1是本發(fā)明日本血吸蟲表皮抗原蛋白的免疫熒光抗體原位雜交實(shí)驗(yàn)結(jié)果示意圖。其中，T代表tegument(表皮)；G代表gut(腸道)；ST代表subtegument(下表皮)，綠色熒光部分是Immunophilin的信號(hào)。
具體實(shí)施例方式
下面結(jié)合附圖和實(shí)施例對(duì)本發(fā)明作進(jìn)一步詳細(xì)的說明。應(yīng)理解，這些實(shí)施例僅用于說明本發(fā)明而不用于限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件，例如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(cè)(New YorkColdSpring Harbor Laboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實(shí)施例1日本血吸蟲表皮抗原蛋白的分離和鑒定實(shí)驗(yàn)1.日本血吸蟲表皮的分離純化血吸蟲表皮的純化按照Triton-x-100提取方法進(jìn)行。表皮抗原蛋白從血吸蟲雄蟲、雌蟲、雌雄混合成蟲和童蟲分離。蟲體用Krebs’-Ringer’s，Tris-馬來酸鹽-緩沖鹽液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液懸浮。這個(gè)液體和蟲體的混和物置于冰水浴中，在搖床上搖動(dòng)10分鐘。然后，裝有液體和蟲體的混和物的試管在渦旋混合器上混旋幾次，然后再放回冰水浴中，靜置使蟲體下沉。用吸管吸出上部液體，放置到另一個(gè)新管中。這個(gè)試管在離心機(jī)上用2500×g的離心力，離心15分鐘。得到的沉淀就是血吸蟲的表皮。Krebs’-Ringer’s溶液的配方是0.15MNaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mMTris-Hepes，pH 7.4。Tris maleate緩沖溶液的配方是0.2M Tris and 0.2MMaleicanhyclride，pH 7.4。
2.日本血吸蟲表皮抗原蛋白的電泳分離日本血吸蟲表皮用裂解劑裂解，蛋白溶液用SDS-聚丙烯酰胺凝膠電泳分離，條帶切割后再做第二維的在線毛細(xì)液相色譜分離以及質(zhì)譜鑒定。軟件分析得到鑒定蛋白。分離好的血吸蟲表皮，用SDS-聚丙烯酰胺凝膠電泳(SDS-PAGE)緩沖液懸浮，用超聲波促進(jìn)組織細(xì)胞裂解和蛋白溶解，超聲波裂解方法為超聲1秒，暫停3秒，總時(shí)間2分鐘。并用100℃加熱5分鐘以使蛋白質(zhì)變性。然后這些表皮抗原蛋白混合物用SDS-PAGE分離，分離方法按照普通的SDS凝膠電泳方法進(jìn)行。膠上電泳泳道用刀切割下來，再用刀均等分割成8-12份。接下來做蛋白的膠內(nèi)酶解。
3.日本血吸蟲表皮抗原蛋白的膠內(nèi)酶解過程膠內(nèi)酶解按以下方法進(jìn)行蛋白膠塊用刀切碎，每個(gè)小塊大小約為1立方毫米。加入100mM NH4HCO3，洗2次；轉(zhuǎn)入到試管中；加入10mM二硫蘇糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反應(yīng)1小時(shí)；冷卻到室溫，離心吸去DTT；加入等體積的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室溫/黑暗反應(yīng)45分鐘；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，真空抽干；加入100mM NH4HCO3，膠膨脹，去掉NH4HCO3，加入乙腈縮膠；去掉乙腈，抽干；加入含Trypsin的消化緩沖液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以剛覆蓋膠為準(zhǔn)；37度，過夜酶切；酶切結(jié)束后，從試管中吸出上清液到新管子中，膠中加入60％ACN/0.5％HAC，在水浴中超聲處理15min，再重復(fù)一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，與前面的上清液合并，真空抽氣去ACN。
4.日本血吸蟲表皮抗原蛋白酶解肽段的在線色譜分離和質(zhì)譜鑒定酶解產(chǎn)生的肽混合物，用真空將溶液體積抽減到30μl。之后，每個(gè)組分的肽混合物上樣到一個(gè)反相捕獲柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脫鹽。然后這個(gè)捕獲柱和一個(gè)分析用的75μm×150mm C18柱(LC Packings)相連接，肽混合物就在200nl/min的流速下，從這個(gè)柱子上洗脫進(jìn)入裝有Protana NanoES的電噴霧離子源的QSTAR pulser i中。Agilent 1100毛細(xì)液相系統(tǒng)(Agilent Technologies)用來提供流動(dòng)相A(0.5％乙酸/水)和流動(dòng)相B(0.5％乙酸/乙腈)，線性梯度是60分鐘內(nèi)5％B到50％B，30分鐘內(nèi)50％B到90％B，然后是在90％B停留15分鐘。在分析柱后通過一個(gè)不銹鋼的兩通(Valco Instrument)連接一個(gè)10-μm直徑的PicoTip納噴霧頭(New Objective)，加上2500-3000伏的噴霧電壓，以獲得穩(wěn)定的噴霧。MS/MS串聯(lián)質(zhì)譜譜圖通過信息依賴的采集(IDA)和任務(wù)-循環(huán)增強(qiáng)來記錄。在每個(gè)測定掃描中，選取3-6個(gè)信號(hào)最強(qiáng)、帶2到3個(gè)電荷的離子，用滾動(dòng)碰撞能量打碎，以獲得串聯(lián)質(zhì)譜的信息。
5.日本血吸蟲表皮抗原蛋白的軟件搜索鑒定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作為內(nèi)標(biāo)，或者以預(yù)先數(shù)據(jù)庫搜索得到的得分高的肽段的分子量，來校正所有的質(zhì)譜譜圖。質(zhì)譜搜索用的數(shù)據(jù)庫，包括從8420個(gè)從EST cluster預(yù)測得到的血吸蟲蛋白質(zhì)序列，以及可能的污染蛋白質(zhì)序列如牛胰酶的序列、宿主蛋白污染如人蛋白質(zhì)序列、小鼠蛋白質(zhì)序列、大鼠蛋白質(zhì)序列、兔子蛋白質(zhì)序列和角蛋白序列等。搜索軟件是MASCOT(http://www.matrixscience.com/，Matrix Science)。搜索參數(shù)為最大漏切點(diǎn)，3個(gè)；monoisotopic；肽電荷數(shù)，+1/+2/+3；修飾，半胱氨酸carbamoylmethylation和methionine氧化。搜索結(jié)果合并后，用下列標(biāo)準(zhǔn)進(jìn)行過濾篩選1.對(duì)每一個(gè)串聯(lián)質(zhì)譜譜圖的搜索結(jié)果列表，只提取排位在第一個(gè)的結(jié)果，其他的則丟棄；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，則這個(gè)肽段丟棄；3.搜索得到的肽段，長度在8個(gè)氨基酸以上的保留，小于8個(gè)氨基酸的丟棄；4.重復(fù)打到的肽段，只計(jì)算其中分值最高的，把這些肽段分值加起來，做為蛋白的得分；5.蛋白的得分超過60，就認(rèn)為是可信的結(jié)果。低分的蛋白，手工檢查后保留或者丟棄。匹配到宿主或者牛胰酶、角蛋白的肽段，則丟棄。
實(shí)施例2日本血吸蟲表皮抗原蛋白的生物信息學(xué)分析和功能注釋1.同源性和相似性分析日本血吸蟲表皮抗原蛋白序列對(duì)GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余數(shù)據(jù)庫(Non-redundant，NR)做BLASTP比對(duì)；日本血吸蟲表皮抗原蛋白序列對(duì)Swissprot數(shù)據(jù)庫做BLASTP同源性比對(duì)。根據(jù)比對(duì)結(jié)果得到的與已知其他物種或已知的血吸蟲蛋白的序列相似性，做出日本血吸蟲表皮抗原蛋白的功能注釋。
2.文獻(xiàn)查檢根據(jù)已經(jīng)發(fā)表的文獻(xiàn)報(bào)道，發(fā)現(xiàn)鑒定得到的25個(gè)表皮抗原蛋白在蟲體上的位置，有一些的確在血吸蟲表皮上。而其他則是新發(fā)現(xiàn)的表皮抗原蛋白。舉例說明，比如以下表皮分離和質(zhì)譜鑒定得到的蛋白，根據(jù)文獻(xiàn)報(bào)道，就是已知的表皮抗原蛋白，而且有些蛋白已經(jīng)有進(jìn)行了疫苗和抗原研究Paramyosin，tropomyosin，myosin，dynein light chain 1，22.6kDa tegument membrane-associated antigen，tegumental antigen Sm20，nitric oxide synthase 1(NOS1)，leucineaminopeptidase，14-3-3 proteins，SnaK，cathepsin B-like cysteine proteaseprecursor，21.7kDa antigen，F(xiàn)K506-binding protein 50(immunophilin)。
實(shí)施例3日本血吸蟲表皮抗原蛋白的免疫熒光抗體原位雜交實(shí)驗(yàn)為了進(jìn)一步用實(shí)驗(yàn)的方法驗(yàn)證用蛋白質(zhì)組學(xué)手段鑒定得到的血吸蟲表皮抗原蛋白，選取了一個(gè)Immunophilin(SjFKBP50，GenBank登錄號(hào)為AY815389)做免疫熒光抗體原位雜交做蛋白定位分析。
實(shí)驗(yàn)方法血吸蟲成蟲用冷凍包埋劑(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗體覆蓋切片，在37℃反應(yīng)60分鐘。該抗體是用重組表達(dá)的Immunophilin免疫小鼠制備的單克隆抗體。隨后切片用PBS洗滌，再加入FITC-偶聯(lián)的兔抗鼠免疫球蛋白的抗體(1∶40稀釋，用含有0.5mg/ml的Evans藍(lán)的PBS稀釋)，置濕盒中37℃反應(yīng)60分鐘。染色結(jié)果用Leica DM-RB型熒光顯微鏡觀察并拍照。
如圖1所示，該實(shí)驗(yàn)結(jié)果證明了Immunophilin確實(shí)分布在血吸蟲成蟲的表皮上。這說明前面表皮分離和質(zhì)譜鑒定的結(jié)果是可信的。
序列表<110>上海人類基因組研究中心<120>日本血吸蟲表皮抗原基因、蛋白及應(yīng)用<130>NP-10281<160>50<210>1<211>2882<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>1taactgttga cattagttac tttcgttatt ggtatcaatg tgtgcgttga atgcagatac60aagtaacgat cccttgtcgg gaaaaaggcg taaacccaat cgcttgattg ttgacgaccc120tataaaagat gataactcag ttgtttattt atctcaggct aaaatggatg agttgcaact180gtttcgcggc gatactgtct tgattaaggg tagaaaaaga agagagacag tctgcgttgc240tcttgtcgat gagacatgcc ctgatgaccg aattcgattc aatcgttgtg ttcgcagcaa300cctacgagtg aggcttggag atattgtcac cacagtgggt tgtcccgata tagtttacgg360aaaacgtatc catgttttgc caatcgatga tacgattgtt ggtctcactg ggaatcttta420tgaagtattt ttgaagccgt actttttagc tgcctaccga cctatacgaa aagatgacat480ttttatcgtg cgcggtggga tgcgagccgt cgaattcaaa gtgattgaaa ccgatccatc540gccgtattgc attgttgccc cggataccat tattcacaca gaaggagatc ctgtaaaacg600agaggatgag gaggaaaagt taaacgaaat aggctacgac gacataggag gttgtcgtaa660gcagctagct caaattaaag aaatggtgga gttacctctt cgtcaccccc agctattcaa720agctattggt gtgaagcccc ctcgtggaat actgctttat ggacctcctg gcactggaaa780gaccttagtt gctcgagcgg ttgccaacga gagcggaagc tttttctttt taatcaatgg840gcctgagatc atgtcaaaat tggccggtga gtcggaatct aatctgcgca aggcattcga900agaggcagaa aaaaatgcac ccgctataat cttcattgac gagcttgatg caatagctcc960taaaagggaa aagactcacg gtgaagtaga gcgtcgcatc gtttctcaat tgctaacatt1020gatggatggt ttgaaacaac gaagccacgt tattgttatg gccgctacaa atcgcccaaa1080tagtgttgat cctgcactca gacgtttcgg gcgatttgac cgggaaatag agatcggtat1140tcctgatagt attgggaggt tagagatttt aaggatacac actaaaaatg tacgactcgc1200taaggacgtt gaccttgtgc agatagcaaa tgaagctcac ggtcatgtgg gagctgactt1260ggcttcgttg tgttctgaag ctgctcttca acaaatacgg aataagatgg atcttattga1320cttagaagat gacactattg acgctgaagt attaaactca ttggctgtga caatggacga1380ctttcgctgg gctctcggga aaagcaatcc ttccgcactt cgtgagacta cagttgaagt1440tcccaacgtg acatgggatg atatcggtgg gctagaaaat gtgaaacgag aacttcagga1500attagttcag tatccagttg aacatcctga taagttccta aaatttggta tgacgcctag1560caagggtgtt ttgttttacg gtcctccagg ttgcggtaag acgttgctag caaaagctat1620tgctagcgag tgtcaagcca attttatcag cattaaaggc cctgaattgc tcactatgtg1680gtttggtgag tctgaggcaa acgtgcggga catttttgac aaggcacggc aggcagcgcc1740
ttgtgtactt tttttcgatg aactggattc cattgccaaa gctcgtggag gcagtgttgg1800agacgctggg ggtgctgctg atcgcgttat caaccaactg ttaacggaaa tggacggtat1860gtccgccaag aaaaatgtgt ttatcattgg cgctaccaat cgaccagaca taattgacgg1920cgccattttg cgtccaggaa gactcgacca actcatctac ataccattac cagatgaacc1980ctcaagagtt aatattctga aggctaatct gcgaaaatca ccgattgcaa aggatgttga2040catcaatttc ttagcaaaag ttacccatgg attttcgggc gctgatctca cagaaatatg2100tcagcgtgcg tgcaaacaag caatacgtga agcaatcgag gcagagatac gtgctgaaag2160tgaaaagaaa aataaaccaa atgctatgga agatgaagat gatcctgtgc cagaaataac2220acgccgtcac tttgaagaag cgatgcgttt cgctagacgc tcagttactg aaaacgatgt2280acggaaatat gaaatgttcg cccagacatt gcagcaatcc agaggaatag gtagtaattt2340cagattcccc gggtcagatg gtcctggaat acctacaggt gcgggtggtc aaggtggtgg2400ccctgttttt ggctctcata atgatgcaga tgatttatac aattgaaaaa gtaacctttc2460cggtgctttg tgtatttaaa gttttagtca aagttatcct gtgtcatcaa tttttacctc2520atgcttctga tttgaacttt aatctccatt ggaaagttat gtgacgtcaa acagattgct2580tctccgtttt taaagattgt tccctggatg atagattgta attcagacaa tttgtacctg2640atatatttcc atatatttaa aaaaaaaaaa aaaaaaatct catgttgttt ttcattttat2700tcagttccct aatcgcatga attttttccc ttactgctca acattttatg atgcgattat2760tacactcatg attcgagtag ttttgattga aatgtttgtt tgcatacctt tcaaacaagt2820atacttactg ataacaagtt aataaacgat ctagaaggta aaaaaaaaaa aaaaaaaaaa2880aa 2882<210>2<211>1065<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>2acatcataca aattacattt agttactaag tgataagatt tcactttaac tttacattat60tcactttatt attctataat tataataata agaataataa taacaatatg gaacatatta120aaaagaagat gttagcaatg aaattagaca aagagaatgc agttgatgaa gctgatcagc180tggaggcaaa attacgtgaa aaagaattgg aaatgcaaac taaagatgaa gaagctgctg240aagttgtcaa gaaaattcaa caagttgata cggataagga aactgcacaa actcaattag300ctgaaactaa taccaaatta gaagaaacag ataaacgtgc aactgaagct gaagctgaag360tcgcctcact gcagaaacgg atccggcaac ttgaagatga attagaatct accgagactc420gccttcaaga agcaaccctt aaacttgaag aggccagtaa ggccgctgat gaaagtgatc480gtggacgtaa agtgttggag aatcgtacat ttgcggatga agaacgtatc aatcaattag540aagaacaatt aaaagaatct acatttatgg cagaagatgc tgatcgaaaa tatgatgagg600cggcacgtaa gttggccatc actgaagtgg aactcgaacg ggctgagtct cgattagaag660ctgccgaaag taaaattact gaactcgaag aagagttacg cattgttggt aataatgtaa720aatctttgga gatttctgaa caagaggcag ctcaacgtga agaggcatat gaagaaaata780ttcgtgattt aactgagcgt ttgaaagctg ctgaggatcg tgctcaagag tctgaaagac840ttgtcaatac tttacaagct gatgctgacc gccttgaaca tgaatcggtt actgataaag900aaaatataaa gcactcagtg aagaattgga ttcaacatcc gctgaactta ctggaaatca960atacaaatat cgattttatc cccctcctct ccgtcattcg ttattgttcc cttccaatta1020
ttatcgacag tattctctcc tccctcctcc ttctccccct cttcc1065<210>3<211>1681<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>3gttgtaagca ttgtgttatc gggttgtatg cgaggacaga taacaaatat tactattaat60attttataaa aagttgaata cagcaactgt cactacgact aaactatggt cgctaaagac120tttattgagc ttttcggaaa attcaactat gctaatcgtg ttgccgttga agatttctca180gatcgactga gtttgtttac agtcgtctta ttcttgatag cttgcatagt tgtatcagcg240aaacaatact ttcttaattc aatctcatgt tacattccgg taaaaccaac tggtgaaaat300tataattcgt atttaactga ttactgttgg gttcatggga ctattccatt aagacctgat360gaaccaatgc caactacacc gaaagaatgg gaacaatatg accaactgag gagaattacc420tattatcaat gggtcccgtt cgttcttggc ctacaatgta tcttctttta tattccacat480attgcatggc aagctgtctg tgcacataga tctggtggag atctgttcgc tcttgtgaag540gcagctgcag atgcagcaat ttccgaacgc ggttctcgta aaagtcaagt taaacgagtt600gctgaatttc tagaagatat gatcgatggt cataaggact gtcggcatgg acgtcgaatg660gattttactc gacgtgcata tgatatgtgt gggatatgtg tagttagtaa gcgtcttgga720acatgcctcg tattttcata catctgtgta aaactcataa ctattatcaa tgcaattatg780caagtatatc tgattcaacg ttttcttggc ttctatgctg atggtagcgc cggacataaa840agtatgcagc tagggaaaac atacgaagct aactccgcag tttcagccat gtcaaaccat900gataatgaag atttggatgg attcggattt ggtttaaccg tagcaaatca tatacgtgct960ggacgtgatt ggccagagac aattcttttc ccacgtgttg cttattgccg tgtaccagga1020atccgtttag tcggttctga gaatgcttat acggcacaat gtgccctgcc tataaatatg1080ttgaatgaga aaatttacat atttttctgg ttctggatat gtttccttat tgctgcaagt1140atattcagtt taattttatg gttaatcaga atggtgatat caccaagacg taaagatttt1200attaaacgtt ttctacgcat taaaggagta cgttcaagga ctggtcaaga aattacacgt1260gctgatttag atgaatttat cgatgattac cttcgtcgag atggtgtctt tttaatacgt1320atgttagcaa tcaatgcagg tgatgtaatc acatcggaaa ttgttatggc tttatatgaa1380cactatattg ataatattta ttctgtggac agtaccccac agaagagtct ggagaaagaa1440ccgttgaaag atggatcagg gataccgcca aattatgtgt aaatttcttg tgcttgtgtt1500tgtcaacatt acggacattt cattttccct aagatactcc tatcattcgt atccctttaa1560gaaaacagac aaataactta attgtaccaa ttattgattg ccatcttgaa atttttacat1620tttttaataa tgagtacaaa atagaatctg ttggagtttt aaaaaaaaaa aaaaaaaaaa1680a1681<210>4<211>864<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>4aagtaataac catttattat agagaaatcg tcaatattgt ataatacaag cagatatccc60gtaaaggagg cgattaggca atttatcttc taagaattct tttcgtataa ctttcaagct120
tcgtaaacac tcgcatagca aagctttcgc caaacttttg aaaaattaat cgtccatttc180tttgtgtatt tatcattttc tgcagtagct acttcgatgg tttaaaacat ggagtcgcgc240tgtttattgg aacacacaag ttgtaactgt gcatccagcg tctacagtgt ctcagcataa300tatcaatagt aaaattactg tctcttaaat gtgctagcaa catgtgccga catggataac360ggcacttgat gtacgttgga caggtgcact cacccgaagt cagatccact tcatatgatt420tcgatccttg caatatcatg aaatactttg ttagtgttgt aatcatagcg acaattggtg480ttgctttatc cttcaacatt cagaatactg atatgcaaaa cacaaaagat atgggtgatt540atcaatttga cgataatgct acctgttcaa tgtgcattaa ttatgtttcg cattggcaaa600aacatttgaa ttcaactaaa gttaaagagc ttcttcgaac ttacgctgaa ataacctgtg660acttctcatt gttattcaga tatcggtgta tacaagtgat gaacaaatac tataatgcat720tcatttcttt catagaaaat catgatgctg aaacaatatg caagtttact ccattctgtt780aaagataaaa catagaagac ttggatatga acttttacaa gattgttttt agtttatttt840aaataaaata atatttattt catc 864<210>5<211>809<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>5gattaagaaa catgtatgca ttaattgact acaaactaca tcaaaataat ggttacaaag60tactggaaga ggggttgggt taatgtgtta atatgttaag tggtagaaaa tcagttgttt120gtgacatttt taaaatattc attgttatca aatcatcaac tactcgctgg tcattgatga180tgaacatcgt cttcattgtt acttcgcata ttatttatta tccaagtacc ttgttttgtt240gtattttcgc aacatttgag aataaagtaa atttcgctgc taagacatta atgtataata300tggcttcttg ttggctttgt atgtgtgaat caagtgaatt acgatccttc gcactgatac360tgtttggtaa atcacacgtc atgggccttc ccttagactg caatttaata aagtactgtt420tcaaagaagc tgtacactgt tcaaactttt ctattacctc cctgggaaag ttttgatagc480gttttcttat ttcctcttta agtaagtcca ttactatgaa aaagtattta ggaaatgttt540cagatctatt tctttttaaa caggacacat atcgctcaat agtttccttt ttatctggat600atctgttttt cagaatccca gtaattattt caagatacct ataagatgtt aacaaaggtc660ctgaataacg aattatacga tcttgcactt cacttatttg ttttaataag ctagtggata720caccatttcg aaatccactt tgtattgatg aatgaatcaa caccagaagt agtgcaataa780tgtacaacaa agaagaagaa gaagatgat 809<210>6<211>1135<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>6taggtctcgg tttccatttc ttctgccatg gtgaagatat tcgttgggaa tcttaaccca60gagtctaaag cttctgatct ccgcaagaaa tttgaagctt ttggcaaggt tacagaatgt120gacgtagtga acaattacgg ctttgtgcat atggaaaaag agtctgaagc cgaagctgca180attgcagggc ttcaaaacgc tattctagac ggggtgaaaa tcaatgtcga acgaagtcat240ggaaaacgag gtggcggtcc tggtcccgca aggcgcagag gcgagggacg ttatcgtgat300
tatcctcctg agagaggccc acggggtatg ccgcgttaca tgaactcagg cccacccccg360cgtgtgggac gctacggtcc tgcttgggac gacggatatg gcggaccata tgctcctccg420ccacgaaaca gccctagggg ttatgattat cccccgaatg gaacaaatgg taggtatccg480ccgctcgatc gtggtgaacc acatagatct atgccacgtg gcccgacccg tgatccactg540gcgtttccac ttaatacagg atatcgtgga cctccagtcc cggaacctat cccttctgga600agggagtacc cttcgaaacc tgccaggcaa ggctatgatt catatggcga attcgaccgg660tatcgtgacc cagtagcatc cgtccctcca cgtgcaaacg attattacga cacttatggt720aattacagca gtggaaatta cgatgactac agagataacg ataggactat ggcaagttat780gattcgggat acgattatag ttactacgat tatggtgtgg atagcaggtc accggctcca840ccgagatcgg ggtacagtta cggtcgtccc taagagtcgt ggcacgaagt acaacgcgaa900gcaacctcaa atcacgtagg caagagacgt ttgcatttat tactcgtttc ctgttcatca960ttcgcatcaa cattaattgg ctagtgtcag caatcttaca ctcgatcgtc ttgcttttgc1020gtttgtagac attaatcgcc cattgtcagt cgtaaatatg acttgtcgtt ttattgcgtc1080acaagaaatc gtctaaattt cctatttatg tttattgaaa tataaattgt gttag 1135<210>7<211>1675<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>7attcattgaa atagtgtagg aaataattca taactagttt gtaatcatta cataaatatt60gtatattcca tccacatttc gataaattca gttgtataaa gattactgga agcttacctg120tgaagaaact ttcgatattt tgatttcaag cttaattaaa ttttaaaaat gtcagacgat180gaacaaagcc cacaaccatc cccagcattt attcccaaag atgaagagca tgacgatgga240aagtctgaag cgaaaattcg tatagaaatg gaagccaagc gtagaaaaga aagagctgaa300gaggaatgga gagaatatga agaatttcgt agaggggaac gtgaaaaaga agaagaagag360atacgtcaat taagagagcg tagagaaaga cgtaaaatgg aacgtgaaga agaggagaaa420cgattagcag aattacgtca aattgaagat caaaaaagac gagcagaaga agaagaaaga480caaagaaaga aacgtgaaga ggaacaacgt aaacgagagg aacgtgaacg caaaaagaaa540gaatgggaag agcgtaaaaa tgctaatcga cctaattttg ttattacaaa aaaagaacaa600ggtgcacaag aaacagaaga agaaaagaaa gaagaagtta caaagtcaaa agaacaatta660gaagctgaaa aacgagctat tttagaacaa cgtatacaac cgctgaatat tagtggtttt720aatgctgatc aactgaagaa taaagctaaa gaactacatg atctaatttg tcggttagag780ggtgatattt atgatttatc acagcgtttc acaagacaaa gttatgatat gcaagaatta840gcagaacgtg cacgtcaaat aaacaaagga ggtaaagcaa aagctatcac aaatgcaccg900gacccattag cagctaaatt cagtggtatt cctcctatgg ttgtaatgta cagtcaatat960gaacgcgtga aagacccaag gtcattcaaa gatcgacgta ccgtttttac gggtgctaat1020tatgcagaag aatatcaacg tattccacct tcacatatgt tagtaatgac agaagaaggt1080tttcaaatag ctggtggtgc caatggaggt ggtggacatt cgactacttc agcacctgaa1140gttcctgtcg aataaataat tatataatcg ttcatcatac cactatattg ttatgaaata1200taattaccac caatcttttc caattccttc gaatttctct ctatctattc ctgttctgtg1260ttcgttttgt ttcagtgtta ccatattcgc ttttatgttc cgagttctta tttttatgtc1320gtaactgaga aataatttac tcattctgtt atcctcctcc cctccgaagc ttttacctaa1380ataaattatg cgattgcttt cccatcttct tagtcattca ctgcgccttc atcttcttcg1440
ttctgttttt tattatgggt tcatcttaac tattatcaat aattaataca tcgtctgaat1500tatattgttg agtttttttc accaaattaa ctaactttca caacagaatc aataaaccga1560actaccctta tctctaatag acagtgtagt ttattgttat tgctactatt acatttgtta1620tgattccctc ttttatgttg aataatttac tttaactaat aaatggccac attct 1675<210>8<211>1559<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>8ctaaattgtt taactgcact actgttatag ttaagaaacg atcatggccg atgaagaagt60ccaagccctt gttgtagaca atggatcagg aatgtgcaaa gctggattcg ctggtgatga120tgcaccaaga gctgtattcc cttccatcgt tggacgacct cgacatcaag gtgtgatggt180tggtatgggt caaaaagaca gttacgttgg tgatgaagcc caatcgaaac gtggtattct240cacattgaaa tatccaattg aacacggtat tgtaacaaac tgggatgata tggagaagat300ctggcatcac acattctaca atgaactacg tgtggctcca gaagaacatc ccgtcctctt360gactgaagct ccattgaatc cgaaagccaa tcgtgagaag atgacccaaa ttatgttcga420gacattcaac acaccagcca tgtacgttgg tatccaggct gtattatcat tgtatgcatc480tggtcgtaca actggtattg tgttggactc tggtgatggt gtgacacaca ctgtgccgat540ctacgaaggt tatgctctgc cacatgcgat cttgcgtctg gatcttgccg gtcgtgactt600gactgactac ctgatgaaga ttttgactga gcgtggttac agcttcacga caacagctga660gagagaaatc gtacgtgata tcaaggagaa attgtgttat gtggctctgg actttgaaca720ggagatggcg actgccgctt ccagctcatc attggagaag agttatgagt tgcctgatgg780tcaagtcatc accattggta acgaacggtt ccgttgtcct gaggcgttat tccaaccgag840cttcttgggt atggaatctg ctggtgttca cgagaccaca ttcaattcaa taatgaaatg900tgatgtggat attcgtaaag atctgtatgc caacacagtg ttgtcaggtg gtacgacaat960gttcccaggt attgctgatc gtatgcagaa agagatcact gcattagcgc cgagtacaat1020gaagattaag atagttgctc cacctgagcg taaatattca gtttggattg gtggttctat1080tttggcgtca ttgtctactt tccaacagat gtggatttcc aaacaagaat atgatgaatc1140tggtcctggt attgttcacc gaaaatgctt ctaaagttat ttatctatat tacttattca1200attattatct tctaatcata catactgtta ctattattat tactattatg agaatactat1260cttgctaagt catttagtca atgcaaacaa tttctttatt gttttaccca tttgttttac1320tagaattata caatgtcatc aacaattacc taagtcaata tatcaattat agcaagaata1380tgaataaaac aaatatgatc aaccaaatcc tatctctttg tttgcaattc tccctcacta1440tccctcttcc ttttcttttg tttcacttct gttcaagctt gaaaaaatag gcgattcttg1500gaaaaaagac attcttagct attattaata aacataattg cttaatttaa aaaaaaaaa 1559<210>9<211>2253<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>9ggtgaatttg ctgcaagatg cctgaaccaa tgtctaccga gcctcaacct gagacatttg60ctttccaagc tgaaattgct cagcttatga gcttgatcat caacaccttt tattccaaca120
aagaaatatt tcttcgtgag ctgatttcta atgctagtga cgctctggac aaggcaaggt180acatgagctt gacaaactct tcagtttttg atactggtga tgatttatac atcaagctga240taccgaacaa ggaggctgga acgttaactg ttcttgatac aggtattggt atgacgaaag300cagatttgat caacaacctt gggacgattg catcttctgg tacgaaggca ttcatggagg360cactgcaagt aggtgcagac atctcgatga ttggtcaatt cggtgtgggt ttctattcag420catacttggt tgctgaccga gtgcaagtgg ttacgaagaa taatgacgat gaccaatata480tgtgggagtc atctgccggc gggtctttca cgattacacc agatagcagt gagatgccac540gacggggtac taaggtaatt ttacatttta aagaagacca gatggagtat cttgaagaac600gtaaaatccg tgagatagtc aaaaaacact ctcaatttat caactaccct atcaaacttg660ttgtcgacaa agagaggacg aaagaggttt ctgacgatga ggcggagaag gaagaaacga720agaacgagtc tgaagaggct gaggataagc cgaaggttga ggatttagat gaagatgaag780aggaagataa taaagacaaa aagaagaaga aaaaggtgac tgagaagtat acggaagaag840agcagcttaa taagctcaga ccgttgtgga ctcgcaatcc agaggacata actgccgaag900agtatggtga gttttacaag tcgttaaaca atgattggga ggaccatttg gcagtgaaac960acttctctgt tgaaggacag ttagaattcc gagcactact gttcgtacct aagcgagcac1020cttttgatat gtttgagggt cgcaagaaaa gaaacaacat aaagctgtat gtccgcagag1080tatttattat ggacaactgt gaagagttga ttccagaata cctaagtttt gttcgtgggg1140ttgtggatag cgaagacttg ccacttaaca tcagccgtga aatgttgcag cagagcaatg1200tgctaaagat gatacgtaag aatctggtca agaagtgcat tgagttgttc gaggagatag1260tcgaagataa ggagaactac aagaagttct atgaacaatt ctctaagaac attaaacttg1320ggatccatga agatagcgtg aacagaaaga aactggcaga attgcttcgc taccagtcaa1380cagctagcgg tgatgaaatg accagcctaa aggaatacgt ttctcgtatg aagcctgaac1440agaaagatat ttactatata acgggtgaga cgaagcaggc ggttgcaaat tctccattta1500ctgagaaact tactcagcgc ggctttgaag tgttgtatat gattgatcct attgacgagt1560attcagtgac acatcttcgt gaatacgacg gcaagaaact ggtttgtgta actaaggacg1620gcctccaatt gcctgaaaac gaggaggaca agaagaagtt cgaggaattg aaggcatctt1680atgagcctct gtgtaaaaat gtccaggaaa ttcttggcaa gagtgtcgaa aaagtgtcta1740tatctaaccg tctaaccagt tcaccatgct gtgttgtcac ctcggagttt ggttggtcag1800caaatatgga aaggatcatg aaggcacagg cccttagaga ctccagtact atgggctata1860tggcagccaa gaaacagttg gagcttaacc cataccaccc aatgatcaag gccttgaaag1920accagttcga gagtggtgat tcgatcaaac ttgctaagga tctcgttcag ctcttgtacg1980acactgcctt gctttcttct ggtttctcgc tgacggaccc gaagatccac gccaagagca2040ttcatcattt ggtctgtatg tgtctggata tacctgatga agagatgaag gggaagtgtg2100ttgctgctga taatggtccg acggtggctc caccagcaga agcaggggat gatgccggaa2160tggaggaagt ggactagccg gttcggtttc tgtatatacc gatttatcga taaattttgc2220tcgttgcaaa aaaaaaaaaa aaaaaaaaaa aaa 2253<210>10<211>808<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>10ctcgtgcccc tcgtccttct ttgctctact tcaacgtgtt tcttttgttg gttcattcat60tttcacaaga taaaaaggat gtgttgaaga agttaaactc tactttactt tcaatggaaa120
acagcttatt aaaagttcaa caaaacattc aaaacactga ccacgaagta aagaattcga180caaagagaat aaataaagta cttgcacctc acggaagaac aattgacgaa tatgtcaaat240gcaaaactaa tgaatttaaa gctgaagaag gtttatctac aaatactaaa ttcttaaaag300agtttattcc tggaactttt cgtgtttata gaaaatacct gactgatgaa gatatacatt360ggaaaaacgc cgctgaagaa cggaaggcaa aatataaaac aatggcctta gagcacactt420cggaaaagtg tgatgaactt gctgatcaat ttcctgaagc gtttgtagaa attcaagaac480ttaaggatca aatatatgaa cgacaaagat acgaattaca atatgattat atgttttata540agtttttcgt gctattagat atttatagaa aagtgcaagc atttgatgaa acacgtgaag600aacatgagca gtgaaaacaa tgtacaacat tgttggttgt tgagtaattg gtgatttctt660ataaacgaat attttggaaa tattacaaat gactgataaa cttttaataa acatttttat720taagtagttt gtagccataa tttggaaata ctttgtaata aataactgta taacctttat780ttcctatata aacacatttt tgttaagc 808<210>11<211>1533<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>11aaatgaagcc atggtcgtct ctggttgttt tcataaaact cctttgttgg ttcctggtgg60acgagttttt gtatggccag gaattcaacg aatagaaaga atgccattaa atacaatgac120tttaattatc gagagcccgc gtatctatac ccaactcgga gttcccatca cggtaactgg180tgttgcacag gttaaaatca atggaagtaa ccaagaaatg ttagcagctg catgtgaaca240atttcttggc aaatcagaaa acgaaatacg tgaaatagct caagaaaccc tcgaaggtca300tcagcgtgct atcatgggca atatgacagt tgaagaaata tacaaggata gaaaaaaatt360ttcaaaagct gtttttgaag tggccagttc agatttagtt aatatgggta tcagtgtagt420tagttataca ctgaaggata taaaagatga tgagggttat ttacgttcac ttggtttagc480gagaacagca caagttaagt gtgatgcacg tatcggtgaa gctgaagcac gtcgtgatgc540tggtattcga gaagcagaag ctgagaaaca gcgtgttgcc ggtaaacttt taaatgatat600tgaaatttca aaatccaaac gtgattttga attacaaaat gctgcttatg aaaaagaagt660acaagcacgt aaagcagaat ctgaattagc ctatgaatta caggcagcca aagttaaaca720acagattaaa gaagaagaaa tgcaaatcac tgtcctcgag aaaactcaac aaattcaagt780ggaagaacta gaaattgtcc gacaggaacg tcatttagat gcaacaatac gtaaaccagc840tgaggctgaa cgttttcgac tagagcgtct tgctgaagcg gatcgtttgc gtttaattgc900tgaggcagaa gcagaagctg aatctatccg gctacgtggt cttgccgaag ctgaagcatt960aaaggcgata gcacatgctg aagcagagca aatgactaag aaagcagaag catggaaaac1020ttatcagaat gttgctaagt tagacatggt attacaaaca ttaccaaaaa ttgctgcaga1080gattagttca ccattaacaa aatgtgataa agtcactatg gtatgcacag gtgatggaga1140tattggtgtt gctaagttaa ctggtgaatt atttacaata atgaatagtt taccacaatt1200aatacattcc atgactggtt tagatattaa taaaagtatt aaaacagtct aatcaaattg1260actattctat tattattatt catctctata gacaatctat gatttacaga aataaaatgt1320tcatataaat atctatgtaa tgagtgcaat tgtttattaa tgtcattatc gtttcgaaaa1380ttatactcta atttcatcag ttgtttctat gtggtttctg atattgttca tattatactt1440tattagatat ctttttgttt acttataagt agtataaata attttactaa ataaatttat1500ttacattttt aaaaaaaaaa aaaaaaaaaa aaa 1533
<210>12<211>1180<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>12agagattgat ggcgaattaa ctattaatat ttgtttggat gcggtgtatt tctgactaac60ttctgtagac aaaaatcatc tgctattcac aggaaaatta ttcatgtatc tcttaatatg120gctttcataa caaatatgat accggagcaa gctgaagtta aagatcgtgc tgatgaactt180ctctctttgt gtaaaaaggc tgttgctgac tgtaataacg tgaaatcgac acttgattca240cttgataaat tacgttataa acaacgcaat tctaaagtta ataaaaatca attgaagtct300ttatacacac aagcaattag tgaagctgaa caacaaaaag ctactctcat ggctgctttg360gaaaaagtat cagagatccg tactttggag tataagttga gaacacacgt tggtccgaaa420agttttcgtc gtggtgtatt gatgtcagtt ttgcaagaaa acgccaaatc aataccactt480tggataggta aatctgctga aagtccacct cctttgtgtg gtgctattgg accatcgcca540aatgttcccg ctgatcctgg cgaccatgtt gccgctcttg tcccagaacc agatgtggta600gctgcagctt gtaatttatc agagggttgt atattagctg aagtcgtttc atataatcca660gataaagaaa tctatgaagt agaggacgtt gacgctgaag aaggaaagat gcgttactcc720ctgtgtcgtt ccaaagttat accattaccc aaatggaaag ctaatccaat tacaaatcct780gaagcaatat tcaagaaagg tgtcactgtt ttagctcttt atccacaaac aacatgtttc840tacaaggcaa ttgtggatga aattccaaca catgtacatg atgaatattc gctttatttt900gaagattcct cctatcctga aggttacgct ccagctatta ggattcctca aagatatgtt960attcactgcc ccgataaaga agcaatgact agtaaacatt tagagaggtc taaaaggcgt1020agtaaatagc cataagtttt agaaagaggc tattattact ccttacaatt tcacattatt1080catttgtgaa cacaagtact tcctttattt tatctgcatt taaatattat tgatacggat1140tgaccaaaat aaattttatt tttaaaaaaa aaaaaaaaaa 1180<210>13<211>1615<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>13attggctttc ttattttgtt atctaattta tgtattcaac gcactggttg atttaaacta60ctaagctgat atggatcctg aaaagttttt aagtttgcaa atgcaaatgc gtgaaaataa120tatggaagta gaaggtttcc taaaagattt tgatgcgtgg aaacaaagag ttgagtcgaa180aagtcagcat ttaagaaact cttctcagcc agaattgcca gctgttcgca atagtttact240gaaaaagcat aaaaagaaag cagctaaaca agccattaac aataaaaata ctggacgaat300caaagcagat gattatcgtg cttgggacaa atttgctaat caggctttag aagatgacga360tgatattggt ttcgatcctg ataagatctc cgttcctcaa aatgatcgag actgtagtag420tgaaacagat gaagaacaag aagatcgaag aagaatagaa ttgtctaagg aagctcgtga480attaggcaac attcgtttta aagaaggaaa actaaatgaa gctattgaac actatacaat540ggctatacga cttgcacctg aagattctac ttcatataca aatcgtgcat taacttatat600caaaacagaa cgttatgcat ctgctgaagc tgattgtaca gctgctttaa aattagatag660aacttctgtc aaggcattct atcggagagc acttgctcgg aaaggtttag gtcatacaag720
tggagcaatt gaagatttaa aagaattgct taaatacaat ccagataata aaactgcctt780aaatgaactc gaagctttaa ttggtagaaa agaagttagc acacctaaat cctctatgcc840ttgtagtagt tcatttcaat cgcgtaatcc aagaaaaatg agacgaatac caattattga900agttggtagt aatgatcata ataaactgat gaaaacatgt acacaaaata ttacaaaatc960tgactgtaat aatatttacg tccaaaataa aaacaatcct attattaaaa aagatgaatt1020tcaaaagaat aacgctttac attcaactct agaggcatcc actatacttt cagcttctag1080aaaaatatct tcacctgata aagaagtgtc gaccggtaat acaacgataa cgtgtataaa1140tgatcctatt aataagcatg aaccgttgcg aaatataaac ttacgcaaac caccatcaaa1200ttggtttcag ctagaacgtg aactacgtga attgtgtcag agaagtactt cttcgcataa1260ttatcttagc aaagaagcaa ttacctattt gtgtaatatt caaccaactg attacgataa1320attattcggt gaaaatatgg acagtgattt tttgtcacgt atgatccaag cattcagtca1380gtcagatcaa ttaaccaatc aacaaattgc agatcgtttg atatttttat ctaaattgcc1440aaggtttgat attgcttgga tgatgactag tgaatcggaa cgtgtaagta tgattaaatt1500tattaaaact ttacaaaatg ataattcaat tagtaaagaa gcattgagac aaatctgtgt1560acagtttgaa tgtgaataaa ctcctatttt tcaaataaaa aaaaaaaaaa aaaaa 1615<210>14<211>2439<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>14gacgtacgcg tatagtattg aggaatgtct gttatcatcc gactgcaaaa cttaccaata60tcagcaaatg ccgccaatat ccgacgtttt ttcagcggtc tgtccattcc cgagggaggt120gtgcatattg tcggaggcac ggaaggtgac gccttcatcg ctttcgctac agatgaagat180gcaagaaaag ccatgttgct tgatcgacag acgatcaacg gggcatctgt tcgtctgttt240ctgagtagta aagccgaaat gcagtcaata attgaatccg caaaaacttc agcattattt300tctagtggaa atcctgtttt gtctcagccg gtttcccaaa agacagagac tatgcatgca360ttagatgtaa cagctcctac gctacccagt tcctttccat cttatcgtaa tggagttcct420cgtgaaccaa ctgatgattc atctaagcac ttttcttacg cttatggaaa gagtgtaact480ccaatattgg atccatctcg tgcacagatt gattcatcag tgcgtaacaa accagataaa540taccctgaaa acttacctcc agaaagatcc caacactcta atcaggaccg agattatgga600tacaacaacc agcctaacat accttcgagg tatgatgata tacgagactt cccatctgct660aatgactacg gatctcggga tgcgtactta aataaccgta gtgacttgcc acatagatct720tatcgcgatg actatgatta cagcaggcat ccgccaccac tttcttctgg tgacagtttt780gtcacaagga aagacagtga gtctaagaat agtccaaggg atccttacac tgatcggcat840tccgcgctcc atgttttgga cgatgtgtca gcagattacg acagatatcg tacccattct900cctaagcatt taatgaccgg ctttctccac atgatgccca gtcacatgat ttcagtcgtg960ggaataatca cccctactca tcagaattca gaagcggcac cagctcagcc tcttgatcgt1020gaaactgcat acactaattc taggttacga gaggaacgtg atttttctgt tagacctccg1080tggcttaatg atgatattcc taatgtagga ccgaagcgtc catttcagtc tcaaccttca1140gactacgaag agtacccgcg caaacgtcgg ccacaacctt catcatattc tcgcccatct1200gatatagttc ccggagaaac tgattatgtt ctccgagttt gcttaccggc cccggaagct1260gggataaaaa ctgtatttga agtattacgt ggggttcaag ttgttccaaa gtggggcatt1320cgagttgaag aagatttgtt gcaaagacct acaggatatg tatttattat gatgacaact1380
cgtgaatctt atgatcgagc actcagcttc aataatcgtc ctcacaaagg agacgtcata 1440aaagtaacaa gatcttcttt gagcgaattt tacaatgtta gcgatacaaa ctttatttta 1500agatgccctc cagaagttgc caagaagctc ccatccattg atcggtcttg ccctcctcat 1560tataacgacg gttgtcttga gcttgctgaa ctgccgtccg ataccactag gatagaagtc 1620gttcggtttt taggtgctcc tggtctgaca tctgaaaatg tcatattagc tcggtttcct 1680ccgaatgata agtctgcacc acgcggtact aacatgatca gggcattagt tactctccct 1740tctgcagagg atattaaaat cctcctttct gcaaagccac gaccatttcg accggatgca 1800ggacttccat cagtgcgcct aacaccgata tcaaagctac aagtgaccgc gtactcaacc 1860ttggcgatta gcgataagtc taaagacaca gaagagccgg tcaagccgac tgttccacgt 1920gatgttgatc cagagcctac caaagcatcc agtggttttt tgacatgtgc ttctgttagc 1980gggttctctc gcgcggtgaa agattctgag gtcttaaatc tctttccgtc catcttaata 2040ccgggagatg ccgttcgaat gtctccaaat tctaatattg ccttcatcga tttcattagt 2100gaaaataact gtcgtcgtgc tgtaagtgag gcatcaactg aaggctctaa ggcaaaacaa 2160gcacatccat ctcttcgtat tgaagctatc tctcgagaag aaatgctgaa ccgtctcggt 2220catgattcta aagatgagca acccaattat gacagaccta gagcacgcga tagagatcca 2280agggagtccc gcattcattg ggacccgcgt cagcgtccac actctaatcc gtttgaaagc 2340cgtcctccta gtattgggag ggcgaagttc gatgactctt actacgatcg tcctacacta 2400cctgttcctt ttgatgacta tcatgactcc tcccgtaga 2439<210>15<211>1639<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>15ccgggcgcag gaatcggacg aggcgcctct ggaacattgg aatggagtct atcgatgcac 60tcaagaagga cttggaactt gctaaagagc agtaaaaatt gttgacaatg atattaagag 120aattacagga cgagacccat ctgataggcg tgtgggagac gtaaacaaca agcgcatacg 180ccctcgaaca gttgggatca accaattaag gccagattca ttggacaaag tgaacgataa 240tgaaatattt caggatatta agcgacccgt tgatgatgac ctaaaagtgt ctattgcatc 300ctctgttgtt cgtgttactg atactcgttc tcggcaagat gcagccaaag aactaaaaaa 360atcggataaa gaaaggggac gtcgaatgct tggcatcctt cagggtacat tgaagcagtt 420tcaagctgaa tctgtagctg ctgtcaagaa acctcaaatg gaaaaacgac gtttagttga 480tgctaaactg gaggccagag caaaagaaga aaaagaaaat ttacgtcgtg aacgcgcaga 540gctgtttcga gctcgacgag aacatcacgt tgaagtcagc atcctgcaac aaaaaatgcg 600attactaagg aagtttgaaa tatggaaaga gcaacaagaa aaaatgaaag gttattgtcg 660tacttcaaaa ccaccatata tattttatcg tcccaaaatt cacaatgaaa aatccaaatg 720gcgcatagaa gcagctgtcc gagctataga tgctctcatt gctcgccgtc ggaaaaaact 780cgaaacagaa ttcgaagaaa cagtacaagc gcgccgtcgt atggcttcaa gtattaataa 840ttcatctaat aataaaaaaa ttaataaatc tgatgatatt gccaagaaga attatgatag 900atctgacccg gaaatgatga tcgatgtccg ggattacatg atatttccag ggaagctaag 960tttgataact ttaatacttc taaaaaatga aattgatgcc actgtttata cttcaccact 1020tccatcgaat actttatccg ctaagaactt tcacaactct catcgtcaag agttggaagc 1080cggtgaatta tcggatacta gtgagacatt tgctttagga gatgaacctc agctgatgga 1140agaagatcaa catcacgcta tctacacaga gtcaccaagt cgtggacaaa aacatagcac 1200
accaccaata ttgtcctcga aacgacctag tgatcatcta tcatcacata ctagcccagc1260acattgtcag ctagctgctc agaaacgtaa gttatgctct gttggcgaat ctcatccact1320tgacaaaaaa gaaaacaaac agaactcggc tgaaactgat tgattttagt gttttatctg1380tgctatgcat caacgaattt atttcctttt ctaatttctt ccgccactta taatcatcat1440ccacatcatc attgcatata agaaatcata tttccgaatt ttgttttcct aataaggaat1500acaatgtatc tagtgtatgt atgcctctct tctccccact tacgtacact tgttttattt1560tttgtgagcc tgtattatga aaaacgttga ataaaactac atatttgcct gtaaaaaaaa1620aaaaaaaaaa aaaaaaaaa 1639<210>16<211>2129<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>16gacgtgcaaa gctttcttta cagccggtaa cctagcagac gtcatgtatg aaaagtatgg60tgataatatg gctagatgca gtacccaaat ggcacatgat ttaagaagaa tacgtgttga120aactgagaag ttttataaaa gtgataatgg gaatgcatat tcaagacgat tcactgtaca180tggtatatca tccgttccag caaaccaact tatgattgaa gagctaaagc aatcggttgc240agcatatttc gaccaacacc accatattaa attgaaatat cccgagcttc catgcgtaaa300agttgatcaa aaacgtgaag tttacatgcc aatggaattg ctcaatattc taccgtatca360agcacccaat gcaagtaaag ctgaagttgc tagtgaggtt gtacgttgtg cggcagttcg420tccacaagaa cgctttcaag agcttcaaac ttttgctaat aacatgctta agtcacatcc480tttaataaat caattcggct tgacagtcca acctcggcct gtggttgtta acgctcgagt540tattcaccca ccaagtgctg ctttcggtcg ttctcatgtt gtaccgctca aggctgggag600ttggacatca cctggcttcc atgatcctgc tggacgagga gttgaactac tttgggcaat660cctttgcgtt ccaccagaca gacgttctca aggtcatata caaaaggtta tgcatgagct720tccgcgagca gctgatcgcg tcggaatgcg ccttagctcc agaccaagtg tgtcacagtg780ccctactggt gaactcaacc ggagattcga tgagttttct agacaaggat gttcgttttt840actacttatt ctttatgatg aatattcata tcctacaatt aaacgtctaa gtgatctcca900aatgggtatt cgaacacaat gtgtaagagg tcgcacgctt gataaaccaa acgtttttcc960aaaccttctt ttaaaactta atggaaaact tggtggtgtc aactggcaaa ttccagacct1020catcaaaaac ggtaatgaac tcatcatggt atttggtgca gatgtcactc acccagctcc1080cactcaaaat cagcaaatcc gtaagtctgt tgcagcagtt attggcagtg tttctcctga1140tcttatgaga tacggagttg ttattcgtca gcaagcaact actgaaaagg gaaataagac1200tgcaagggaa ataattgatg atatgcgcct aatcgtcaag gaactgttgc agctttattt1260gcgaaatacc aacggtcgct ttccgaatcg tatgatattt taccgcgatg gcgtgtcaga1320aggacaattt gagaatgtcc tagttgaaga gcttgcagca atccagagag catgcgcaga1380tgtccgccct ggtgaggagc ctgctataac atacatcgtc gtacaaaagc gccatcatat1440tcgtttcaaa ccttctgatc ccagagcacg gaatgtggaa ccaggaacaa tcgtggacac1500ggaaataact catccaagag aattcgactt ttatttgtgc tctcaagacg gaattcaggg1560tacgtccaaa cctgcacatt accatgtgtt atatgatgac agtaattgga catcggatgc1620tttgcaaatg ttcacttatt atttatgtta cgcctatatg agatgctcac gtagcgtttc1680ttatccagca ccaacctatt attcacattt agccgctttt cgcgctcgag attggttgtc1740gggaatggat caaccatctg ctttattgga ttgcggtcgc ttcaaagttc atatgtcgca1800
ggtcgatggg atgttttatc tgtagagcta caaagatttt gctataaatc atattatatt1860ttcataaatt tcatattttc tttacttagt cttttcactt aatgtcgcgc aactcttatt1920gttgcttcat ttatgtatct gctgtgaaat atatcataga ttgtttgaat attaatttag1980aaacgaaagc atgcaaaata agtagttgac gacagttgta acacttagtt tgtgtgcagt2040tgactcctgt catcaataaa gaaacaccta ttttttttta gcagcaaata caatcagata2100attggttagt caaaaaaaaa aaaaaaaaa 2129<210>17<211>1475<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>17cttgcgttac atcatgaaga gacaatgtgc tggagaatta gatgcgacct ccaagtccaa60gatgcggcaa acgactcttt tctcaactaa aaatctttca gattcaataa gggaagagtt120gtggaaaacg acaacttcct tatttgtcta tacacatccc aaatgtttac ccagtagcaa180aattttgggc ttagatatgg atggaacaat tattattcca tcttcgggaa aggttttccc240aaaagattat acagattgga agttgattaa cgacaatgtt atccctaaac ttaaggaata300ttttgaaaaa ggatacaaaa ttgtttttct atctaatcaa cgaggtataa caaaaggcta360ccaagatata cacagtttca agttaaaaat acagaatatt gtagataaac tgaacctacc420agttcaggcc atattttcca tcttagatga taagaatcgt aaacctctca ctggtatgtg480ggagtacctt gaaaacgatg gtaattctgg aatccttatt gattcatctg catctttgta540ctctggtgat gcagctggtc gtttagcatt tggaaaacgt aggaaagatc attcatgttg600tgatcgttta tttgctctta atattgggat ccgcttttta acaccagaac aactatggtt660tgatcaagtg gatactgaaa aatttgaaat gcctaaattc aatcctaccg aattgttaac720taatggtata cataaaaata ataatagtaa gttgccaata attcatcggc caactaaacc780tgaactcatc ctaatggtgg gatatcctgc atctggcaaa tctcactttt gtaatcaggt840gttgattcca ttgggatatg aagtaattag tagggataat attggtacat ggcaaaaatg900tgtacaggct gttgaacaag ctacttctaa aagtcttcca gttgtagtcg ataatacaaa960catggatgta gaatcacgag cacgttatat taagattgcc aaagtatggg atataccagt1020gcgttgtttc atcatggaga caactattga acatgctcaa cataatgaac gttttcgttc1080tctaaccaac ttatctcata aaccaatcag tcaaatggtc ttcaacatga tgagatcaaa1140atatgaaaaa ccaaccatag aagaaggcta tcaagaaatt gtaactatcc cgtttatatt1200agataaaaat atggaaaatc tttcactata ttgtaaatac ttattagaaa agtaattacc1260aagattgttt tcttcattat ttgtcaactc attcaacatg gttatgtatt tttgcaacac1320ttatacaaat atataaattg cagtaatatt ttatgtgaac tgggtctctc ctaatcgttg1380ttttcatgtt gtgtctatca tctatacata agctgatcaa ctacagattt ttttcaattt1440tcctttttga ttatgaaata aaatatttat ttcga 1475<210>18<211>1071<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>18catgacacaa gtttaagttt tctcttgaac agttatcttt aaccgggatg acaacgtttg60
cttcacgctt gtgtggttat tccgtagttg gtgatactta caaggctgca ttagatcttt120ataactgggt caagaaaaag gagtctgttg gtccaattgt gcaaacagct gaagataaag180ctatctctgt tataaaacca tttgttgaga gtgggatttt tcagaagatc gatgatatgg240cctgtcaaca tgtattggat cgtgctgagg ctgcttgctt acaaattaag aacacctcga300atgagcgcct tattttgcct actgcaaacc gtgccgtgac aattgccgag gcatgcgcca360acttatatct accgaaagat gatgatccaa agtttgctgc agggggtgat gctacgccaa420cagagcgact tgtgagactg caacacagaa catcaacaca tgctgttaca ttaattcatt480ctacagttga tcatgctaat tcattactgt ctacccttgc cacatacggt agtaatttaa540atcaaacagt ttcaaattct gttataaaaa atacacttac tcaggctatt gctttatatg600aattagctta ttctactgca aaggctgtaa gcctacctat agctgcacgt tgtcttagta660tcattctcga tcatgttaga aaactagacg accaaattaa agaatcaaaa ggaataaact720ggatggattt gaagaaaata gtgacacttc ttgaacacat gaagggaaga ttaaatggac780aggaggcagt cacagaaatc gaactcgtgc caatcactga aaagccaata tcatctcttg840tttctggtgg taaaaataaa tcagataaaa gtgtggacag ttcataaata tgctcattga900tttttttatc atctttattt tccactgcaa catgtgaatc gatttccttt cataaactct960actgatgata atttgcttgt gatttttctg cataattctg attgatatct tttatattag1020tattcattga tttcatttcg ttctttgttc agaaaatata aatttgttac t 1071<210>19<211>930<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>19gtttaaaggg aacaaagttg ggttcccccg cgggggcggc cctttaaaaa ttggtgctcc60cccgggcccc aggatttggc caggaggcgc ggccgtttga taaatcgaaa agacttgttt120atggccgtgt taagagtgtt gtgcggactt ctgctggttt ccattttgtt tcttgggttt180gtcctcagtg aagcaaatgg gccgaaagta acagaaaaag tgttctttga tattgaagtt240gataaacaac ctctcggtcg aataatcatc ggattatttg gtaaaacagt accgaagact300gtggaaaatt tcaagcaact ttctattggt acaacgttga aagatggtcg aactgccgca360tataaaggaa gcacttttca tcgtgtcata aagtcattta tgatacaggg tggtgatttt420acaaatcatg atggaactgg cggctttagt atttatggtg agcggtttcc cgatgagaat480ttcaaactaa aacatgttgg tgcagggtgg ctgtcgatgg ctaatgctgg tcctaacaca540aatggagccc aatttttcat cacgactaca aaaaccccgt ggttggatgg aaaacatgtt600gttttcggaa aagttgtaga agggatgagt gttgttagac aaattgaaaa catgcagacg660gactctagag ataggcctgt taaaagtgtg aagatagcaa actgtggaca cattcctgtt720gatgtaccgt tttcagtatc caacactgac gctgttgaat gaactcggat gaagaatttt780ctattttctt gtatttatat ttatacgtaa atataattta ggataatggg aaaaaccaat840aatatgattt attgacggaa ttggacgtaa aacccccgag ggggggccgg tacccaatct900cgcccttaag ggcggcctat acacacttcc 930<210>20<211>782<212>DNA<213>日本血吸蟲(Schistosoma japonicum)
<400>20acataatttg tttcacgaca tactggtcag tcaattcaag ttcagcccct ctacgtattt60gcttgtttat caaatcagcc gataccttgt catctgaaca taatgaagat aaacattgat120ggcctactgg tctattttcc gtatgaatat atatatccag aacagtacca ctacatgatt180gagttgaaac gtactttaga tgctaaaggt catggtgtct tggaaatgcc atctgggact240gggaaaacag tctcattact ctcattaata gttgcctata tgaaagctcg tcctggaata300gttgaaaaat ttgtttattg ttcacgtaca gtacctgaat tagagaaagt cattgatgaa360ttaaaagtcc ttgataaata ttatgcagat gaaactaatg aaaaaggatg tggtctttta420ggaattattc ttagttcgcg taaaaatcta tgcattcacc gcgatgtaaa acatgctggt480gatggagctg ctgtagattc cgcctgcttt cgactaactg ctagttttgt acgtaaaaat540cgtatagctg atcctaatgt tgcgtactgt aagtactatg aggaattcga tttaaacggc600cgggataatc cttttgcccc ggggatttac agtatggcag atatcaaagc ttatgctaaa660aacataacta ttgcccttac tttcttacaa aaatgctatt tcctatgcaa atattatcgt720ctacagctac ttttacctac tcgatccaaa atagcaaatg tcgctctcgg aattacccaa780aa 782<210>21<211>709<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>21tctattatct aaggtaacat caatctgtat atacagtagt atacatattg acgaaacaaa60atgaagattg cattagcact tttattcata ctacaattgc attcgatcca acttcaactt120attagtggta gtaaagtatc agcagatagc aacagtcgta gcaaaggaaa caatatagaa180accaaaaatg atgaaaaatc aatgagtcat aaaaaacatt tggagaataa taggatgctg240acaaaatatg aaaagaaaat aagaaaagaa tttaatcaaa atctgaaaag aatagaaacg300atggtaaaaa acatgacaga tgtattgaca acgatgacaa aattaattaa agatgtgcca360agaaaaaacc ttatttatgt gcgtttatat taaacaaaaa ttttcagaca atcgaaatta420tagaattatt tgaaaacaag ttgccactat tgaattacat aaattatcag cttcaaaatg480ttaagtcatc ctaatcaatt tgttgtcaga cgtttttaca actacgtagt atgacatatt540ttaggtgatg ttcaatgtct gaattcttgt aatccttcct tactttttta ctactttttg600atgtgttttt tgaaaatctt tcattttact gcttattttg taaatggtta ttttatattg660tcttatctgt ttgtttaaat aggtatttcg gtagatattt aaacgacta709<210>22<211>880<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>22tacagatgac tggaattctc actattcatc acatggggtt agcctttaag tgtgtttcga60gaggattatc agtttctgtt catcgccttc gagctgacgt ggttgtcatt ggatcgggtc120ctggtggcta cgttgcatct attaaagctg cacaactagg catgaagact gtatgcgtag180aaaaaaatga tactctagga ggaacctgct tgaatgttgg ttgtattcca agcaaatcgt240tattgaacaa ctcacattta tatcaccttg ttcattctga tgaaatgaaa catcggggga300
tagatattga gggtttcaag cttaatctac ctggtatgat gaaggccaaa gaaaagtctg360tttcaagctt gaccggtgga atagcatact tatttaaaca aaacaagatt gatcatatca420atggtgtcgg ctcaatagtt aatcctaacg aagttttagt tacaaaagca gatgggagtg480aagaacgaat ttccactgga gcgtattctc attgcaacag ggagtgaggt tactcccgtt540cccgggaatt gagatcgatg aacagtttat cgtatcaagc actggtgcat tatctctaac600taaggttcct caacatcttg tagtcatcgg agctggagtt attggtgttg aattaggctc660tgtgtggaag cgactaggtg ctgaagtcac ttgtgtggag tttcttggtc atgttggtgg720tatgggtatc gatattgaaa taggcaaaaa ctttcaaaaa attttgacta aacagggtct780gaaattcctg ttaagtacta aagttttatc ggcttcgaga tctggagata ctatcacagt840gcaattagaa agtgtgaaag atggaaaatc tcacagtatc 880<210>23<211>847<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>23aatttgcgta tcaacaatgg atggtcgtgc cgagttgtat gaagaagtaa aactttatcg60taatgcacgc gaaagagaaa agtatgataa tcttgctgaa ctctatgctg ttatcaacac120catacaatgc ttacaaaaag catatattaa agatcatgtg gaatcaacag agtatactgc180tgcctgctcg aagcttcttg tccagtatga ggctgcattt aagcaggttc aaggagatga240atttagcact gtggaggatt ttatgcggaa atacaaaatg gattgtccgg cagctttgga300gcgtattaaa gagggaagac caataactat taaggataat aaacaaaata taaacaagtg360tatcgctgat actgtttctt tatttattac ggttatggat aaactgcgat tagaaattcg420tgcagtggat gaaattcacc cagatcttcg agaattgtat gaaactctat gtcggttgag480cattttacct ccagattttg agggtaaaga tagagttaaa gtttggttag ataaaatgga540tcagatgaaa gcttccgatg aattgtctga tacggaagca cgccaaatgc tttttgattt600ggaatcttca tataatgctt tcaatccgga cgttacattc tggccagtga ataccagagt660ttatatttgt atacgtatgg ggtcacctat acttgcataa tataagcttc gaagggtgaa720gacattttat aaataattcc ttttaatggg gattattttc tttcatctca atttgggggg780ggaaggtaac agcctttcga taagaaatta aaaaatattt tgggggtaaa aaaaaaaaaa840aaaaaaa 847<210>24<211>502<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>24cctttttgtc atcgtcgtca tttggcgcga tgcctcccaa agttgtttct gggaaagcag60ctaaaaagtc atctaaagtc aaagccccta agtctgaaaa aaagaaaaaa cgaaggagga120aagagagtta tgccatatac atatataagg tcttacgaca agtccatcct gacacgggga180tttcgtcgaa ggcaatgtct attatgaatt catttgtgaa tgatatcttc gaaaggattg240cagcagaagc tagtcgtctg gcacattata ataaaaaatc cactataacc agtcgtgagg300ttcaaactgc agtccggttg ctattacctg gtgaacttgc taaacatgct gtttctgagg360gcacaaaagc tgttactaaa tacagtggat caaagtaaaa tgattccatc tcctctgtaa420
tgttttcaag cagccaataa acctacttcc atcaccccaa acaaacaaaa gaaacaactt480ccgcaacgcg gtggtggtgg gt 502<210>25<211>1701<212>DNA<213>日本血吸蟲(Schistosoma japonicum)<400>25ctaatactga cgggatacct gattgtaaca atgaatctac cactctacaa aatgtcctgt60tgtccagggt tatctgctat atgcgtgctg agacgtggcg tttacaacgc tgcagcctcc120ggcctggctg cacaacgaaa tgatgtttta atcactcaaa gtgcagaagg gataaagctt180gtcagtttac ctcagccatc acttggcctt ggatgtgcac gagttgcact agtcgtaaaa240tccggtccac gatgtgaatc ctcgaaaaac cgtggaatca gtcatttaat gcgtcgctca300tttggaattt ccactcctga attaactagt gttaatttga caagacatct gcaacagatg360ggtgcgcgtg tacaatgcac tacaacccgt gaacatatga tctacacagt agatgtcgca420ccgaatttcg ctgtccgtgc gggttacctc ttgtgctcta tggcatcagc ttcgtgttac480tattcttggg aactaaacga tattgtctac aagttaatga gaaaagatgt tgatactcta540aaccggcgaa atttaagtgg attgggtatg gaattgttac atgaagcggc ttttggtact600agcgactcag gatgtggttt aggatattca ttaatttcac cagttgatcg catcggatct660catttaatcg atcaaataaa cgaatatcat tcacgtgctt ttgtcgggga gaaatgtgtt720tcgggcattg ttcattcgcg cgctgatgta gatggaattg atatattgaa acaggtgaca780agtagtatta accttaatcc tccacattta gaggctagtt cggataatca tggctttgtt840ggaggcgaga tacgtcgtga tctaattgct gcgtccacag tgtacgctta tttagcgtgg900ccctcccgtg ggttctggcc tgtttgtgat ctgatcgtct gtgcactgaa tgggtcatcc960aatcgcatac accatggtgg gaatgcttca aaatccttgt tagcccgtac cgctatagaa1020ggagacattg atactgaagc tgtagctttc cataaggtat atagcgatca tggtctcttt1080gggattgctg tagctggatc ttgtcctaaa acagttggtt ctaggataaa gcgtatcatt1140tcagttttac gatcagcaaa tttcaccgag gagaatctca aacaagcaaa acaaattctg1200agagctgacc taatgttccg atatgaaaat ccattccatt cacttgtgga tatttcgact1260aatttacttt caccaaccaa ccaatctgta aagccgattg aagtagttgc tagtgtgaac1320aaaactgact taaaatcttt taatgatgcc ataaataaaa ttgtgacgag taatcatgct1380gctttttcat tggttggtcc taatctaggt tcaattgaac ctcttcacgt attgcttaaa1440gaataattat gtatgtgaac catcttgtta gactagtatg cttctaatct cctacacctt1500tttttctctc actaaacaaa tgtggtagaa gattttttgt taattaataa taacttttag1560aagactttat gtaatcttaa gtctttagtt taaataatgg tcgttttcat ttctccatgc1620taaaaagcaa ctagtgtgaa atacttaagc tttatatttt ttaggaatat actgttttaa1680gtaatatatt ataattgaac g 1701<210>26<211>802<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>26
MCALNADTSNDPLSGKRRKPNRLIVDDPIKDDNSVVYLSQAKMDELQLFRGDTVLIKGRK60RRETVCVALVDETCPDDRIRFNRCVRSNLRVRLGDIVTTVGCPDIVYGKRIHVLPIDDTI120VGLTGNLYEVFLKPYFLAAYRPIRKDDIFIVRGGMRAVEFKVIETDPSPYCIVAPDTIIH180TEGDPVKREDEEEKLNEIGYDDIGGCRKQLAQIKEMVELPLRHPQLFKAIGVKPPRGILL240YGPPGTGKTLVARAVANESGSFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFI300DELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSVDPALRRFGRF360DREIEIGIPDSIGRLEILRIHTKNVRLAKDVDLVQIANEAHGHVGADLASLCSEAALQQI420RNKMDLIDLEDDTIDAEVLNSLAVTMDDFRWALGKSNPSALRETTVEVPNVTWDDIGGLE480NVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIASECQANFISIK540GPELLTMWFGESEANVRDIFDKARQAAPCVLFFDELDSIAKARGGSVGDAGGAADRVINQ600LLTEMDGMSAKKNVFIIGATNRPDIIDGAILRPGRLDQLIYIPLPDEPSRVNILKANLRK660SPIAKDVDINFLAKVTHGFSGADLTEICQRACKQAIREAIEAEIRAESEKKNKPNAMEDE720DDPVPEITRRHFEEAMRFARRSVTENDVRKYEMFAQTLQQSRGIGSNFRFPGSDGPGIPT780GAGGQGGGPVFGSHNDADDLYN 802<210>27<211>319<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>27MEHIKKKMLAMKLDKENAVDEADQLEAKLREKELEMQTKDEEAAEVVKKIQQVDTDKETA60QTQLAETNTKLEETDKRATEAEAEVASLQKRIRQLEDELESTETRLQEATLKLEEASKAA120DESDRGRKVLENRTFADEERINQLEEQLKESTFMAEDADRKYDEAARKLAITEVELERAE180SRLEAAESKITELEEELRIVGNNVKSLEISEQEAAQREEAYEENIRDLTERLKAAEDRAQ240ESERLVNTLQADADRLEHESVTDKENIKHSVKNWIQHPLNLLEINTNIDFIPLLSVIRYC300SLPIIIDSILSSLLLLPLF 319<210>28<211>458<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>28MVAKDFIELFGKFNYANRVAVEDFSDRLSLFTVVLFLIACIVVSAKQYFLNSISCYIPVK60PTGENYNSYLTDYCWVHGTIPLRPDEPMPTTPKEWEQYDQLRRITYYQWVPFVLGLQCIF120FYIPHIAWQAVCAHRSGGDLFALVKAAADAAISERGSRKSQVKRVAEFLEDMIDGHKDCR180HGRRMDFTRRAYDMCGICVVSKRLGTCLVFSYICVKLITIINAIMQVYLIQRFLGFYADG240SAGHKSMQLGKTYEANSAVSAMSNHDNEDLDGFGFGLTVANHIRAGRDWPETILFPRVAY300CRVPGIRLVGSENAYTAQCALPINMLNEKIYIFFWFWICFLIAASIFSLILWLIRMVISP360RRKDFIKRFLRIKGVRSRTGQEITRADLDEFIDDYLRRDGVFLIRMLAINAGDVITSEIV420MALYEHYIDNIYSVDSTPQKSLEKEPLKDGSGIPPNYV 458<210>29
<211>137<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>29MYVGQVHSPEVRSTSYDFDPCNIMKYFVSVVIIATIGVALSFNIQNTDMQNTKDMGDYQF60DDNATCSMCINYVSHWQKHLNSTKVKELLRTYAEITCDFSLLFRYRCIQVMNKYYNAFIS120FIENHDAETICKFTPFC 137<210>30<211>127<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>30MLSGRKSVVCDIFKIFIVIKSSTTRWSLMMNIVFIVTSHIIYYPSTLFCCIFATFENKVN60FAAKTLMYNMASCWLCMCESSELRSFALILFGKSHVMGLPLDCNLIKYCFKEAVHCSNFS120ITSLGKF 127<210>31<211>281<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>31MVKIFVGNLNPESKASDLRKKFEAFGKVTECDVVNNYGFVHMEKESEAEAAIAGLQNAIL60DGVKINVERSHGKRGGGPGPARRRGEGRYRDYPPERGPRGMPRYMNSGPPPRVGRYGPAW120DDGYGGPYAPPPRNSPRGYDYPPNGTNGRYPPLDRGEPHRSMPRGPTRDPLAFPLNTGYR180GPPVPEPIPSGREYPSKPARQGYDSYGEFDRYRDPVASVPPRANDYYDTYGNYSSGNYDD240YRDNDRTMASYDSGYDYSYYDYGVDSRSPAPPRSGYSYGRP 281<210>32<211>328<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>32MSDDEQSPQPSPAFIPKDEEHDDGKSEAKIRIEMEAKRRKERAEEEWREYEEFRRGEREK60EEEEIRQLRERRERRKMEREEEEKRLAELRQIEDQKRRAEEEERQRKKREEEQRKREERE120RKKKEWEERKNANRPNFVITKKEQGAQETEEEKKEEVTKSKEQLEAEKRAILEQRIQPLN180ISGFNADQLKNKAKELHDLICRLEGDIYDLSQRFTRQSYDMQELAERARQINKGGKAKAI240TNAPDPLAAKFSGIPPMVVMYSQYERVKDPRSFKDRRTVFTGANYAEEYQRIPPSHMLVM300TEEGFQIAGGANGGGGHSTTSAPEVPVE328<210>33<211>376<212>PRT<213>日本血吸蟲(Schistosoma japonicum)
<400>33MADEEVQALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ60SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM120TQIMFETFNTPAMYVGIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAILRLD180LAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKS240YELPDGQVITIGNERFRCPEALFQPSFLGMESAGVHETTFNSIMKCDVDIRKDLYANTVL300SGGTTMFPGIADRMQKEITALAPSTMKIKIVAPPERKYSVWIGGSILASLSTFQQMWISK360QEYDESGPGIVHRKCF376<210>34<211>719<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>34MPEPMSTEPQPETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKARYMSLTN60SSVFDTGDDLYIKLIPNKEAGTLTVLDTGIGMTKADLINNLGTIASSGTKAFMEALQVGA120DISMIGQFGVGFYSAYLVADRVQVVTKNNDDDQYMWESSAGGSFTITPDSSEMPRRGTKV180ILHFKEDQMEYLEERKIREIVKKHSQFINYPIKLVVDKERTKEVSDDEAEKEETKNESEE240AEDKPKVEDLDEDEEEDNKDKKKKKKVTEKYTEEEQLNKLRPLWTRNPEDITAEEYGEFY300KSLNNDWEDHLAVKHFSVEGQLEFRALLFVPKRAPFDMFEGRKKRNNIKLYVRRVFIMDN360CEELIPEYLSFVRGVVDSEDLPLNISREMLQQSNVLKMIRKNLVKKCIELFEEIVEDKEN420YKKFYEQFSKNIKLGIHEDSVNRKKLAELLRYQSTASGDEMTSLKEYVSRMKPEQKDIYY480ITGETKQAVANSPFTEKLTQRGFEVLYMIDPIDEYSVTHLREYDGKKLVCVTKDGLQLPE540NEEDKKKFEELKASYEPLCKNVQEILGKSVEKVSISNRLTSSPCCVVTSEFGWSANMERI600MKAQALRDSSTMGYMAAKKQLELNPYHPMIKALKDQFESGDSIKLAKDLVQLLYDTALLS660SGFSLTDPKIHAKSIHHLVCMCLDIPDEEMKGKCVAADNGPTVAPPAEAGDDAGMEEVD 719<210>35<211>203<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>35RAPRPSLLYFNVFLLLVHSFSQDKKDVLKKLNSTLLSMENSLLKVQQNIQNTDHEVKNST60KRINKVLAPHGRTIDEYVKCKTNEFKAEEGLSTNTKFLKEFIPGTFRVYRKYLTDEDIHW120KNAAEERKAKYKTMALEHTSEKCDELADQFPEAFVEIQELKDQIYERQRYELQYDYMFYK180FFVLLDIYRKVQAFDETREEHEQ 203<210>36<211>413<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>36MVVSGCFHKTPLLVPGGRVFVWPGIQRIERMPLNTMTLIIESPRIYTQLGVPITVTGVAQ60VKINGSNQEMLAAACEQFLGKSENEIREIAQETLEGHQRAIMGNMTVEEIYKDRKKFSKA120
VFEVASSDLVNMGISVVSYTLKDIKDDEGYLRSLGLARTAQVKCDARIGEAEARRDAGIR180EAEAEKQRVAGKLLNDIEISKSKRDFELQNAAYEKEVQARKAESELAYELQAAKVKQQIK240EEEMQITVLEKTQQIQVEELEIVRQERHLDATIRKPAEAERFRLERLAEADRLRLIAEAE300AEAESIRLRGLAEAEALKAIAHAEAEQMTKKAEAWKTYQNVAKLDMVLQTLPKIAAEISS360PLTKCDKVTMVCTGDGDIGVAKLTGELFTIMNSLPQLIHSMTGLDINKSIKTV 413<210>37<211>303<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>37MAFITNMIPEQAEVKDRADELLSLCKKAVADCNNVKSTLDSLDKLRYKQRNSKVNKNQLK60SLYTQAISEAEQQKATLMAALEKVSEIRTLEYKLRTHVGPKSFRRGVLMSVLQENAKSIP120LWIGKSAESPPPLCGAIGPSPNVPADPGDHVAALVPEPDVVAAACNLSEGCILAEVVSYN180PDKEIYEVEDVDAEEGKMRYSLCRSKVIPLPKWKANPITNPEAIFKKGVTVLALYPQTTC240FYKAIVDEIPTHVHDEYSLYFEDSSYPEGYAPAIRIPQRYVIHCPDKEAMTSKHLERSKR300RSK 303<210>38<211>502<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>38MDPEKFLSLQMQMRENNMEVEGFLKDFDAWKQRVESKSQHLRNSSQPELPAVRNSLLKKH60KKKAAKQAINNKNTGRIKADDYRAWDKFANQALEDDDDIGFDPDKISVPQNDRDCSSETD120EEQEDRRRIELSKEARELGNIRFKEGKLNEAIEHYTMAIRLAPEDSTSYTNRALTYIKTE180RYASAEADCTAALKLDRTSVKAFYRRALARKGLGHTSGAIEDLKELLKYNPDNKTALNEL240EALIGRKEVSTPKSSMPCSSSFQSRNPRKMRRIPIIEVGSNDHNKLMKTCTQNITKSDCN300NIYVQNKNNPIIKKDEFQKNNALHSTLEASTILSASRKISSPDKEVSTGNTTITCINDPI360NKHEPLRNINLRKPPSNWFQLERELRELCQRSTSSHNYLSKEAITYLCNIQPTDYDKLFG420ENMDSDFLSRMIQAFSQSDQLTNQQIADRLIFLSKLPRFDIAWMMTSESERVSMIKFIKT480LQNDNSISKEALRQICVQFECE 502<210>39<211>805<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>39MSVIIRLQNLPISANAANIRRFFSGLSIPEGGVHIVGGTEGDAFIAFATDEDARKAMLLD60RQTINGASVRLFLSSKAEMQSIIESAKTSALFSSGNPVLSQPVSQKTETMHALDVTAPTL120PSSFPSYRNGVPREPTDDSSKHFSYAYGKSVTPILDPSRAQIDSSVRNKPDKYPENLPPE180RSQHSNQDRDYGYNNQPNIPSRYDDIRDFPSANDYGSRDAYLNNRSDLPHRSYRDDYDYS240RHPPPLSSGDSFVTRKDSESKNSPRDPYTDRHSALHVLDDVSADYDRYRTHSPKHLMTGF300LHMMPSHMISVVGIITPTHQNSEAAPAQPLDRETAYTNSRLREERDFSVRPPWLNDDIPN360
VGPKRPFQSQPSDYEEYPRKRRPQPSSYSRPSDIVPGETDYVLRVCLPAPEAGIKTVFEV420LRGVQVVPKWGIRVEEDLLQRPTGYVFIMMTTRESYDRALSFNNRPHKGDVIKVTRSSLS480EFYNVSDTNFILRCPPEVAKKLPSIDRSCPPHYNDGCLELAELPSDTTRIEVVRFLGAPG540LTSENVILARFPPNDKSAPRGTNMIRALVTLPSAEDIKILLSAKPRPFRPDAGLPSVRLT600PISKLQVTAYSTLAISDKSKDTEEPVKPTVPRDVDPEPTKASSGFLTCASVSGFSRAVKD660SEVLNLFPSILIPGDAVRMSPNSNIAFIDFISENNCRRAVSEASTEGSKAKQAHPSLRIE720AISREEMLNRLGHDSKDEQPNYDRPRARDRDPRESRIHWDPRQRPHSNPFESRPPSIGRA780KFDDSYYDRPTLPVPFDDYHDSSRR 805<210>40<211>325<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>40MLGILQGTLKQFQAESVAAVKKPQMEKRRLVDAKLEARAKEEKENLRRERAELFRARREH60HVEVSILQQKMRLLRKFEIWKEQQEKMKGYCRTSKPPYIFYRPKIHNEKSKWRIEAAVRA120IDALIARRRKKLETEFEETVQARRRMASSINNSSNNKKINKSDDIAKKNYDRSDPEMMID180VRDYMIFPGKLSLITLILLKNEIDATVYTSPLPSNTLSAKNFHNSHRQELEAGELSDTSE240TFALGDEPQLMEEDQHHAIYTESPSRGQKHSTPPILSSKRPSDHLSSHTSPAHCQLAAQK300RKLCSVGESHPLDKKENKQNSAETD325<210>41<211>593<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>41MYEKYGDNMARCSTQMAHDLRRIRVETEKFYKSDNGNAYSRRFTVHGISSVPANQLMIEE60LKQSVAAYFDQHHHIKLKYPELPCVKVDQKREVYMPMELLNILPYQAPNASKAEVASEVV120RCAAVRPQERFQELQTFANNMLKSHPLINQFGLTVQPRPVVVNARVIHPPSAAFGRSHVV180PLKAGSWTSPGFHDPAGRGVELLWAILCVPPDRRSQGHIQKVMHELPRAADRVGMRLSSR240PSVSQCPTGELNRRFDEFSRQGCSFLLLILYDEYSYPTIKRLSDLQMGIRTQCVRGRTLD300KPNVFPNLLLKLNGKLGGVNWQIPDLIKNGNELIMVFGADVTHPAPTQNQQIRKSVAAVI360GSVSPDLMRYGVVIRQQATTEKGNKTAREIIDDMRLIVKELLQLYLRNTNGRFPNRMIFY420RDGVSEGQFENVLVEELAAIQRACADVRPGEEPAITYIVVQKRHHIRFKPSDPRARNVEP480GTIVDTEITHPREFDFYLCSQDGIQGTSKPAHYHVLYDDSNWTSDALQMFTYYLCYAYMR540CSRSVSYPAPTYYSHLAAFRARDWLSGMDQPSALLDCGRFKVHMSQVDGMFYL 593<210>42<211>413<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>42MKRQCAGELDATSKSKMRQTTLFSTKNLSDSIREELWKTTTSLFVYTHPKCLPSSKILGL60DMDGTIIIPSSGKVFPKDYTDWKLINDNVIPKLKEYFEKGYKIVFLSNQRGITKGYQDIH120
SFKLKIQNIVDKLNLPVQAIFSILDDKNRKPLTGMWEYLENDGNSGILIDSSASLYSGDA180AGRLAFGKRRKDHSCCDRLFALNIGIRFLTPEQLWFDQVDTEKFEMPKFNPTELLTNGIH240KNNNSKLPIIHRPTKPELILMVGYPASGKSHFCNQVLIPLGYEVISRDNIGTWQKCVQAV300EQATSKSLPVVVDNTNMDVESRARYIKIAKVWDIPVRCFIMETTIEHAQHNERFRSLTNL360SHKPISQMVFNMMRSKYEKPTIEEGYQEIVTIPFILDKNMENLSLYCKYLLEK 413<210>43<211>279<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>43MTTFASRLCGYSVVGDTYKAALDLYNWVKKKESVGPIVQTAEDKAISVIKPFVESGIFQK60IDDMACQHVLDRAEAACLQIKNTSNERLILPTANRAVTIAEACANLYLPKDDDPKFAAGG120DATPTERLVRLQHRTSTHAVTLIHSTVDHANSLLSTLATYGSNLNQTVSNSVIKNTLTQA180IALYELAYSTAKAVSLPIAARCLSIILDHVRKLDDQIKESKGINWMDLKKIVTLLEHMKG240RLNGQEAVTEIELVPITEKPISSLVSGGKNKSDKSVDSS 279<210>44<211>213<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>44MAVLRVLCGLLLVSILFLGFVLSEANGPKVTEKVFFDIEVDKQPLGRIIIGLFGKTVPKT60VENFKQLSIGTTLKDGRTAAYKGSTFHRVIKSFMIQGGDFTNHDGTGGFSIYGERFPDEN120FKLKHVGAGWLSMANAGPNTNGAQFFITTTKTPWLDGKHVVFGKVVEGMSVVRQIENMQT180DSRDRPVKSVKIANCGHIPVDVPFSVSNTDAVE 213<210>45<211>226<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>45MKINIDGLLVYFPYEYIYPEQYHYMIELKRTLDAKGHGVLEMPSGTGKTVSLLSLIVAYM60KARPGIVEKFVYCSRTVPELEKVIDELKVLDKYYADETNEKGCGLLGIILSSRKNLCIHR120DVKHAGDGAAVDSACFRLTASFVRKNRIADPNVAYCKYYEEFDLNGRDNPFAPGIYSMAD180IKAYAKNITIALTFLQKCYFLCKYYRLQLLLPTRSKIANVALGITQ 226<210>46<211>110<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>46MKIALALLFILQLHSIQLQLISGSKVSADSNSRSKGNNIETKNDEKSMSHKKHLENNRML60TKYEKKIRKEFNQNLKRIETMVKNMTDVLTTMTKLIKDVPRKNLIYVRLY 110
<210>47<211>173<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>47MTGILTIHHMGLAFKCVSRGLSVSVHRLRADVVVIGSGPGGYVASIKAAQLGMKTVCVEK60NDTLGGTCLNVGCIPSKSLLNNSHLYHLVHSDEMKHRGIDIEGFKLNLPGMMKAKEKSVS120SLTGGIAYLFKQNKIDHINGVGSIVNPNEVLVTKADGSEERISTGAYSHCNRE 173<210>48<211>227<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>48MDGRAELYEEVKLYRNAREREKYDNLAELYAVINTIQCLQKAYIKDHVESTEYTAACSKL60LVQYEAAFKQVQGDEFSTVEDFMRKYKMDCPAALERIKEGRPITIKDNKQNINKCIADTV120SLFITVMDKLRLEIRAVDEIHPDLRELYETLCRLSILPPDFEGKDRVKVWLDKMDQMKAS180DELSDTEARQMLFDLESSYNAFNPDVTFWPVNTRVYICIRMGSPILA 227<210>49<211>122<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>49MPPKVVSGKAAKKSSKVKAPKSEKKKKRRRKESYAIYIYKVLRQVHPDTGISSKAMSIMN60SFVNDIFERIAAEASRLAHYNKKSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYSG120SK 122<210>50<211>471<212>PRT<213>日本血吸蟲(Schistosoma japonicum)<400>50MNLPLYKMSCCPGLSAICVLRRGVYNAAASGLAAQRNDVLITQSAEGIKLVSLPQPSLGL60GCARVALVVKSGPRCESSKNRGISHLMRRSFGISTPELTSVNLTRHLQQMGARVQCTTTR120EHMIYTVDVAPNFAVRAGYLLCSMASASCYYSWELNDIVYKLMRKDVDTLNRRNLSGLGM180ELLHEAAFGTSDSGCGLGYSLISPVDRIGSHLIDQINEYHSRAFVGEKCVSGIVHSRADV240DGIDILKQVTSSINLNPPHLEASSDNHGFVGGEIRRDLIAASTVYAYLAWPSRGFWPVCD300LIVCALNGSSNRIHHGGNASKSLLARTAIEGDIDTEAVAFHKVYSDHGLFGIAVAGSCPK360TVGSRIKRIISVLRSANFTEENLKQAKQILRADLMFRYENPFHSLVDISTNLLSPTNQSV420KPIEVVASVNKTDLKSFNDAINKIVTSNHAAFSLVGPNLGSIEPLHVLLKE 47權(quán)利要求
1.一類日本血吸蟲表皮抗原基因，其編碼具有日本血吸蟲表皮抗原蛋白質(zhì)活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.25所示的序列；(b)SEQ ID No.1～SEQ ID No.25所示的序列中每條序列的互補(bǔ)序列；(c)與SEQ ID No.1～SEQ ID No.25所示的序列中每條序列有至少70％同源性的序列，及(d)上述(a)～(c)中一條或數(shù)條的組合。
2.如權(quán)利要求1所述的一類日本血吸蟲表皮抗原基因的核苷酸序列，其特征在于所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.25所示的序列。
3.一類日本血吸蟲表皮抗原蛋白，包括(a)具有SEQ ID No.26～SEQ ID No.50所示氨基酸序列的蛋白；(b)由(a)的蛋白發(fā)生一個(gè)或幾個(gè)氨基酸置換、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
4.如權(quán)利要求3所述的日本血吸蟲表皮抗原蛋白，其特征在于，其具有SEQ IDNo.26～SEQ ID No.50所示的氨基酸序列。
5.一類抗體，其特征在于，所述抗體能與權(quán)利要求3所述的蛋白特異性結(jié)合。
6.權(quán)利要求3所述的日本血吸蟲表皮抗原蛋白在制備血吸蟲疫苗中的應(yīng)用。
7.權(quán)利要求3所述的日本血吸蟲表皮抗原蛋白在制備防治血吸蟲病的藥物中的應(yīng)用。
全文摘要
本發(fā)明公開了一類日本血吸蟲表皮抗原基因及其編碼蛋白，還公開了與該日本血吸蟲表皮抗原蛋白特異結(jié)合的抗體。此外，本發(fā)明還公開了此類日本血吸蟲表皮抗原蛋白在制備血吸蟲疫苗中的應(yīng)用，以及在制備防治血吸蟲病的藥物中的應(yīng)用。運(yùn)用本發(fā)明的日本血吸蟲表皮抗原蛋白，可研究血吸蟲治療藥物的作用靶點(diǎn)，從而在開發(fā)研制預(yù)防或治療血吸蟲病的藥物過程中發(fā)揮重要作用。
文檔編號(hào)C07K14/44GK1986806SQ200510111729
公開日2007年6月27日 申請(qǐng)日期2005年12月20日 優(yōu)先權(quán)日2005年12月20日
發(fā)明者劉鋒, 韓澤廣, 王志勤, 胡薇, 馮正 申請(qǐng)人:上海人類基因組研究中心, 中國疾病預(yù)防控制中心寄生蟲病預(yù)防控制所