專利名稱：使用經(jīng)遺傳修飾的布拉霉屬生物產(chǎn)生類胡蘿卜素或其前體的方法、由所述方法產(chǎn)生的類 ...的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及使用經(jīng)遺傳修飾的布拉霉屬(Blakeslea)生物產(chǎn)生類胡蘿卜素或其前體的方法，涉及由所述方法產(chǎn)生的類胡蘿卜素或其前體，并涉及其用途和供給，特別是作為高純度類胡蘿卜素，作為包含產(chǎn)類胡蘿卜素生物和至少一種類胡蘿卜素的食品，特別是作為動物飼料、動物飼養(yǎng)添加劑和食品添加劑的用途和供給，本發(fā)明還涉及通過所述的方法獲得的類胡蘿卜素的用途，用于生產(chǎn)化妝品、藥物、皮膚病學(xué)制劑、食品或食品添加劑。
三孢布拉霉(Blakeslea trispora)是已知的β-胡蘿卜素(Ciegler，1965，Adv Appl Microbiol.71)和番茄紅素(EP 1201762，EP 1184464，WO03/038064)生產(chǎn)生物。
三孢布拉霉的多種DNA序列是已知的，特別是編碼由香葉基香葉基焦磷酸合成β-胡蘿卜素的類胡蘿卜素生物合成基因的DNA序列(WO 03/027293)。
用布拉霉菌生產(chǎn)番茄紅素和β-胡蘿卜素獲得高產(chǎn)量使得這種生物特別適合發(fā)酵生產(chǎn)類胡蘿卜素。
進(jìn)一步提高先前已經(jīng)天然產(chǎn)生的胡蘿卜素及其前體的產(chǎn)量以及實(shí)現(xiàn)生產(chǎn)其他類胡蘿卜素，比如以前從布拉霉菌中分離的即使得到也獲得量很少的葉黃素也是令人感興趣的。
將類胡蘿卜素加入飼料、食品、食品添加劑、化妝品和藥物中。類胡蘿卜素尤其用作著色的色素。除此之外，類胡蘿卜素的抗氧化作用和這些物質(zhì)的其他性質(zhì)得到利用。類胡蘿卜素分為純烴類即胡蘿卜素類和含氧烴類即葉黃素類。葉黃素類如角黃素和蝦青素用來例如對雞蛋和魚類著色(Britton等，1998，類胡蘿卜素，Vol.3，Biosynthesis and Metabolism)。胡蘿卜素類中的β-胡蘿卜和番茄紅素尤其用于人類營養(yǎng)學(xué)。β-胡蘿卜素例如用作飲料的著色劑。番茄紅素有預(yù)防疾病的作用(Argwal和Rao，2000，CMAJ 163739-744；Rao和Argwal 1999，Nutrition Research19305-323)。無色的類胡蘿卜素前體八氫番茄紅素特別適合用作化妝品、藥物或皮膚病學(xué)制劑中的抗氧化劑。
上述應(yīng)用中作為添加劑的大多數(shù)類胡蘿卜素及其前體是通過化學(xué)合成法制備的。所述化學(xué)合成很復(fù)雜而且生產(chǎn)成本很高。相反，發(fā)酵方法相對簡單并基于廉價的起始原料。如果先前發(fā)酵方法的產(chǎn)量得到提高或用已知生產(chǎn)生物能得到新的類胡蘿卜素，用發(fā)酵方法生產(chǎn)類胡蘿卜素及其前體會是經(jīng)濟(jì)的并能和化學(xué)合成法競爭。
這需要遺傳工程，即對布拉霉菌進(jìn)行特定的遺傳修飾，特別是如果欲使其生產(chǎn)葉黃素時，因為野生型布拉霉菌是不能天然合成這些化合物的。
例如，先前有兩種方法通過三孢布拉霉發(fā)酵生產(chǎn)八氫番茄紅素(i)用化學(xué)試劑如MNNG隨機(jī)誘變法可以產(chǎn)生不能將八氫番茄紅素轉(zhuǎn)化成番茄紅素并進(jìn)一步轉(zhuǎn)化為β-胡蘿卜素的三孢布拉霉突變體(Mehta和Cerda-olmedo，1995，Appl.Microbiol.Biotechnol.42836-838)。
(ii)通過添加八氫番茄紅素脫氫酶的抑制劑，如二苯胺和肉桂醇，可以阻斷八氫番茄紅素的進(jìn)一步轉(zhuǎn)化，造成八氫番茄紅素積累(Cerda-Olmedo，1989，在E.Vandamme編輯的Biotechnology ofvitamin，growth factor and pigment production.LondonEisevier AppliedScience，一書中的27-42頁)。
然而，上述通過三孢布拉霉生產(chǎn)八氫番茄紅素的方法有許多缺點(diǎn)。
所述隨機(jī)誘變法通常不僅影響用于進(jìn)一步轉(zhuǎn)化八氫番茄紅素的類胡蘿卜素生物合成基因，而且還影響其他重要基因。由于這一原因，突變體的生長和合成特性經(jīng)常受到削弱。因此，如果可行的話，通過隨機(jī)誘變番茄紅素或β-胡蘿卜素高生產(chǎn)者獲得例如八氫番茄紅素高生產(chǎn)者也只能通過大量復(fù)雜的實(shí)驗才能實(shí)現(xiàn)。添加抑制劑增加了生產(chǎn)成本并有可能造成產(chǎn)品污染。另外，抑制劑也可削弱細(xì)胞生長，從而限制了類胡蘿卜素或其前體的產(chǎn)生，特別是八氫番茄紅素的產(chǎn)生。
上述隨機(jī)誘變法和添加抑制劑法的缺點(diǎn)可以通過遺傳工程修飾避免。
然而，迄今為止，還沒有對布拉霉菌，特別是對三孢布拉霉進(jìn)行遺傳修飾，如特定的遺傳改造方法的報道。
在一些情況下已成功應(yīng)用的用于產(chǎn)生經(jīng)遺傳修飾真菌的方法是農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化法。例如，通過這種方法，以下生物已經(jīng)被農(nóng)桿菌轉(zhuǎn)化釀酒酵母(Saccharomyces cerevisiae)(Bundock等，1995，EMBO Journal，143206-3214)、泡盛曲霉(Aspergillus awamori)、構(gòu)巢曲霉(Aspergillusnidulans)、黑曲霉(Aspergillus niger)、盤長孢狀刺盤孢(Colletotrichumgloeosporioides)、腐皮鐮孢(Fusarium solani pisi)、粗糙脈孢菌(Neurospora crassa)、里氏木霉(Trichoderma reesei)、糙皮側(cè)耳(Pleurotus ostreatus)、禾谷鐮孢(Fusarium graminearum)(van derToorren等，1997，EP 870835)、二孢蘑菇(Agraricus bisporus)、Fusariumvenenatum(de Groot等，1998，Nature Biotechnol.16839-842)、禾生球腔菌(Mycosphaerella graminicola)(Zwiers等，2001，Curr.Genet.39388-393)、Glarea lozoyensis(Zhang等，2003，Mol.Gen.Genomics268645-655)、米赫毛霉(Mucor miehei)(Monfort等，2003，F(xiàn)EMSMicrobiology Lett.244101-106)。
在欲導(dǎo)入基因和細(xì)胞DNA之間有盡可能多的序列同源性引起的同源重組是特別令人感興趣的，因此就有可能在受體生物基因組中位點(diǎn)特異性導(dǎo)入或去除遺傳信息。另外，供體DNA可通過非位點(diǎn)特異性的異?；蚍峭粗亟M整合入受體生物基因組。
農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化和隨后轉(zhuǎn)入基因的同源重組已經(jīng)在以下生物中得到檢測泡盛曲霉(Gouka等.1999，Nature Biotech 17598-601)、Glarealozoyensis(Zhang等，2003，Mol.Gen.Genomics 268645-655)、禾生球腔菌((Zwiers等.2001，Curr.Genet.39388-393)。
另外一種已知的用于轉(zhuǎn)化真菌的方法是電穿孔法。Hill，Nucl.Acids.Res.178011一文說明了通過電穿孔法整合轉(zhuǎn)化酵母。絲狀真菌的轉(zhuǎn)化也由Chakaborty和Kapoor進(jìn)行了描述(1990，Nucl.Acids.Res.186737)。
“生物彈射擊的(biolistic)”方法即通過DNA運(yùn)載顆粒轟擊細(xì)胞轉(zhuǎn)移DNA，例如對哈茨木霉(Trichoderma harzianum)和綠粘帚菌(Gliocladium virens)已有報道(Lorito等，1993，Curr.Genet.24349-356)。
然而，先前并沒有成功地用這些方法對布拉霉菌特別是三孢布拉霉進(jìn)行特定的遺傳修飾。
在遺傳修飾布拉霉菌和三孢布拉霉時特別困難的事實(shí)是它們的細(xì)胞在有性繁殖周期和無性細(xì)胞周期的全過程是都多核的。例如，在三孢布拉霉菌株NRRL2456和NRRL2457的孢子中，平均每個孢子有4.5個細(xì)胞核(Metha和Cerdá-Olmedo，1995，Appl.Microbiol.Biotechnol.42836-838)。因而，遺傳修飾通常只存在于一個或很少幾個細(xì)胞核，也就是說這些細(xì)胞是異核體的。
經(jīng)遺傳修飾的布拉霉菌特別是三孢布拉霉如果欲用于生產(chǎn)時，遺傳修飾在生產(chǎn)菌株的全部細(xì)胞核中都存在是重要的，特別是在基因刪除的情況下，這樣才有可能實(shí)現(xiàn)穩(wěn)定和高合成特性并不會有副產(chǎn)品。因此這些菌株在所述的遺傳修飾方面必須是同型核的。
產(chǎn)生同型核細(xì)胞的方法僅在布拉克須霉(Phycomyces blakesleanus)中有報道(Roncero等，1984，Mutat.Res.125195)。根據(jù)所述報道中描述的方法，通過加入誘變劑MNNG(N-甲基-N’-硝基亞硝基胍)來去除細(xì)胞核，以統(tǒng)計學(xué)上獲得只有一種功能核的一定數(shù)量的細(xì)胞。這些細(xì)胞隨后接受篩選，使得只有帶有隱性選擇標(biāo)記的單核細(xì)胞可以長成菌絲體。這些篩選后細(xì)胞的子代是多核的，并且是同型核的。布拉克須霉隱性篩選標(biāo)記的一個例子是dar。與dar陰性菌不同，dar陽性菌可以吸收有毒的核黃素類似物5-碳-5-脫氮核黃素(Delbrück等，1979，Genetics 9227)。通過加入5-碳-5-脫氮核黃素(DARF)來篩選隱性突變體(recessive mutant)。
然而，這一方法還未用于布拉霉菌，特別是三孢布拉霉，特別沒有該方法關(guān)于轉(zhuǎn)化或產(chǎn)生類胡蘿卜素或其前體的報道。
也可以從天然來源中進(jìn)行分離。已知獲得八氫番茄紅素的例子是從番茄、胡蘿卜或棕櫚油等中提取類胡蘿卜素、維生素E和也包括八氫番茄紅素的其他成分的混合物。這里的問題是如何把各種分別的類胡蘿卜素同其他的類胡蘿卜素分離開。因此，例如，通過這個方法不能獲得八氫番茄紅素的純品。特別是，植物中天然存在的類胡蘿卜素量是低的。
相反，發(fā)酵方法相對簡單并基于廉價的起始原料。如果先前發(fā)酵方法的產(chǎn)量得到提高或用已知生產(chǎn)生物能得到新的類胡蘿卜素，用發(fā)酵方法生產(chǎn)類胡蘿卜素是經(jīng)濟(jì)的并能和化學(xué)合成法競爭。然而，發(fā)酵產(chǎn)生類胡蘿卜素的問題是已建立的方法僅能提供少量的高純度類胡蘿卜素。而且，如果使用大量的溶劑，發(fā)酵過程通常需要很多步驟。因此就產(chǎn)生了大量的廢物或者要費(fèi)很大的勁進(jìn)行再循環(huán)過程。
事實(shí)上，已知多種微生物可以產(chǎn)生類胡蘿卜素。例如WO 00/13654 A2中公開了從杜氏藻(Dunaliella sp.)的藻類中提取八氫番茄紅素和六氫番茄紅素的混合物。該方法也沒有產(chǎn)生八氫番茄紅素純品，并且后者必須從其他產(chǎn)物分離。而且，所述藻類未經(jīng)過遺傳修飾，并且它們必須通過加入抑制劑來影響其生物合成。
WO 98/03480 A1也公開了三孢布拉霉作為β-胡蘿卜素的產(chǎn)生生物。這里，通過提取法從三孢布拉霉生物量中獲得到β-胡蘿卜素晶體。然而，所述的方法需要大量的不同溶劑通過多個提取和洗滌步驟來得到高純度的晶體。獲得的β-胡蘿卜素量基于所用的生物量也是小的。
WO 01/83437 A1公布了從酵母中提取蝦青素的方法，該方法包括用微波輻射處理培養(yǎng)肉湯來滅菌和破壞細(xì)胞。根據(jù)該方法，需要用微波輻射的方法破壞細(xì)胞以從酵母中獲得并不破壞蝦青素。然后，再用甲醇、乙醇或丙酮或其混合物提取蝦青素。然而，這需要大量的溶劑(5到20份溶劑比1份懸浮液)和長時間(24小時)。此外，蝦青素的純度沒有說明并且獲得的量很少。然而，本申請人的實(shí)驗和其他出版物證明通過甲醇或乙醇的方法提取是不可能的。
WO 98/50574同樣也公開從微生物生物量中分離類胡蘿卜素晶體，并且在這里，與WO 01/83437 A1形成對比的是，用甲醇、乙醇、丙酮只是用于從生物量中去除脂類，即用來洗滌。因此，用來提取類胡蘿卜素的溶劑是乙酸乙脂、己烷或油。隨后還需要用大量的乙醇和水進(jìn)行多個純化和洗滌步驟，導(dǎo)致了純度僅有93.3％且產(chǎn)量僅有35％。
WO 03/038064 A2描述了通過共培養(yǎng)突變的三孢布拉霉交配型(-)和不需加入類胡蘿卜素生物合成抑制劑就能產(chǎn)生番茄紅素的三孢布拉霉交配型(+)來發(fā)酵產(chǎn)生番茄紅素。用于發(fā)酵的突變體是通過非選擇性的化學(xué)突變及隨后的篩選產(chǎn)生的。培養(yǎng)肉湯經(jīng)過破壞細(xì)胞處理，隨后用不同的水性介質(zhì)通過改變鹽濃度和pH來進(jìn)行純化，并用例如乙酸乙酯、己烷和1-丁醇的不混溶有機(jī)溶劑去除脂類。另一種描述的可選辦法是用大量的乙酸乙脂提取。關(guān)于純度的信息是缺少的。因為乙酸乙脂和己烷是番茄紅素的溶劑，可以推測部分的番茄紅素會被洗掉，從而減少了可能的理論產(chǎn)量。
WO 01/55100 A1也大體描述了對破裂的生物量用多步洗滌和純化的步驟而不使用溶劑提取的方法從生物量中分離類胡蘿卜素特別是β-胡蘿卜素。這涉及用水、堿液、酸、丁醇和乙醇洗滌破裂了的三孢布拉霉生物量，所以需要用大量不同的溶劑和水性介基。所得的β-胡蘿卜素純度為96-98％。但是，沒有關(guān)于產(chǎn)量的信息。
WO 97/36996 A2概括性地描述了從微生物中分離物質(zhì)(尤其是類胡蘿卜素)的方法，所述物質(zhì)用固/液體提取方法從生物量分離。這里顯然不需要破裂細(xì)胞，但生物量要首先用擠壓的方法使其變成顆粒和多孔狀。該方法僅用來分離類胡蘿卜素的可能性和其純度或產(chǎn)量的信息沒有描述。擠壓形成的殘渣隨后可以用作飼料添加劑。
在上述所有的方法中，需要使用大量的溶劑進(jìn)行提取，以通過完全的提取來提高分離的類胡蘿卜素量，和/或?qū)⒋罅康乃越榛糜诩兓拖礈臁＿@造成了高成本和可能需要復(fù)雜的再循環(huán)方法和根據(jù)需要使用復(fù)雜的再循環(huán)廢物的方法。
而且，營養(yǎng)豐富的培養(yǎng)肉湯和其中存在的生物量在提取或分離類胡蘿卜素后被當(dāng)作廢物處理。除了這些表面的缺點(diǎn)，上述方法還有另一個決定性的缺點(diǎn)，那就是隨后必須將類胡蘿卜素加入食品中，即事實(shí)上它們并不是食品的一部分或者在其中僅以不充足的量存在。因此，如果在食品中類胡蘿卜素含量是食品本身所含有的，這將有很大的優(yōu)勢。
同樣，進(jìn)一步提高先前已經(jīng)天然存在的胡蘿卜素及其前體的產(chǎn)量以及產(chǎn)生其它從野生型微生物中即使產(chǎn)生和分離也獲得量很少的類胡蘿卜素，例如葉黃素尤其優(yōu)選地蝦青素或玉米黃素，以及八氫番茄紅素或紅木素是必要的。
本發(fā)明的目的是提供經(jīng)遺傳修飾的能夠產(chǎn)生類胡蘿卜素或其前體，特別是葉黃素尤其是蝦青素或玉米黃素、八氫番茄紅素或紅木素的布拉霉屬菌株，特別是三孢布拉霉菌株。而且，本方法欲使經(jīng)修飾細(xì)胞與其野生型相比具有提高的類胡蘿卜素產(chǎn)量。本方法也欲使新細(xì)胞或由其產(chǎn)生的菌絲體以經(jīng)濟(jì)學(xué)上令人感興趣的量產(chǎn)生先前從天然存在的真菌中不可獲得的類胡蘿卜素或其前體，特別是葉黃素尤其優(yōu)選地是蝦青素或玉米黃素、八氫番茄紅素或紅木素。本文中，該方法欲使布拉霉屬菌株特別是三孢布拉霉經(jīng)過遺傳修飾并能產(chǎn)生同核的經(jīng)遺傳修飾的生產(chǎn)菌株。
而且，該方法欲實(shí)現(xiàn)產(chǎn)生其它從野生型微生物中即使產(chǎn)生和分離也獲得量很少的類胡蘿卜素，例如葉黃素，尤其優(yōu)選地是蝦青素或玉米黃素，以及八氫番茄紅素或紅木素。
本發(fā)明的目的也是欲得到從遺傳修飾的布拉霉菌特別是三孢布拉霉中產(chǎn)生需要使用更少量的溶劑和基本上不產(chǎn)生廢物并獲得高純度和更高產(chǎn)量類胡蘿卜素的方法。
就此而言，本發(fā)明欲利用很大部分存在于發(fā)酵罐中的營養(yǎng)物，包括類胡蘿卜素和其他存在于微生物中的營養(yǎng)物。
因此，提供產(chǎn)生其本身含有所需類胡蘿卜素而不是作為添加劑的含類胡蘿卜素食品的方法也是本發(fā)明的目的。特別地，通過該方法獲得的食品中營養(yǎng)物含量至少應(yīng)等同于先前獲得的食品中營養(yǎng)物含量。該方法欲使獲得的類胡蘿卜素得到有效的利用。
該目標(biāo)是通過使用經(jīng)遺傳修飾的布拉霉屬生物產(chǎn)生類胡蘿卜素或其前體的方法實(shí)現(xiàn)的，該方法包括以下步驟(i)轉(zhuǎn)化至少一個細(xì)胞，(ii)步驟(i)中獲得的細(xì)胞任選地同型核轉(zhuǎn)變以產(chǎn)生這樣的細(xì)胞，其中細(xì)胞核的一個或多個遺傳性狀都經(jīng)同樣方式進(jìn)行了修飾并且所述遺傳修飾在細(xì)胞中得到顯現(xiàn)，以及(iii)篩選和繁殖經(jīng)遺傳修飾的細(xì)胞，(iv)培養(yǎng)經(jīng)遺傳修飾的細(xì)胞，(v)制備由經(jīng)遺傳修飾的細(xì)胞產(chǎn)生的類胡蘿卜素或由所述經(jīng)遺傳修飾的細(xì)胞產(chǎn)生的類胡蘿卜素前體。
本發(fā)明的方法使布拉霉屬菌以特定的和穩(wěn)定的方式得到遺傳修飾，以獲得能夠產(chǎn)生類胡蘿卜素或其前體，特別是葉黃素尤其優(yōu)選地是蝦青素或玉米黃素，以及八氫番茄紅素或紅木素的具有一致細(xì)胞核的細(xì)胞菌絲體。細(xì)胞優(yōu)選地為三孢布拉霉種真菌的細(xì)胞。這里產(chǎn)生的類胡蘿卜素或其前體基本上沒有污染，并且在其培養(yǎng)基中可能獲得高濃度的所述類胡蘿卜素或其前體。
轉(zhuǎn)化是指將遺傳信息轉(zhuǎn)移至生物體內(nèi)，特別是真菌體內(nèi)。這包括技術(shù)人員熟知的任何可能導(dǎo)入所述信息，特別是DNA的方法，例如用DNA-運(yùn)載顆粒轟擊、用原生質(zhì)體轉(zhuǎn)化、DNA的顯微注射、電穿孔、接合或轉(zhuǎn)化感受態(tài)細(xì)胞、化學(xué)物質(zhì)或農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化。遺傳信息是指基因片段、基因或多個基因。遺傳信息可通過例如載體的幫助或以游離核酸(如DNA，RNA)的形式、以其他任何方式導(dǎo)入細(xì)胞，并通過同源重組整合入宿主基因組或以游離形式存在于細(xì)胞中。這里特別優(yōu)選同源重組。
優(yōu)選的轉(zhuǎn)化方法是通過根癌農(nóng)桿菌(Agrobacterium tumefaciens)介導(dǎo)的轉(zhuǎn)化法。為此，欲轉(zhuǎn)移的供體DNA首先插入到載體中，此載體(i)攜帶位于欲轉(zhuǎn)移DNA側(cè)翼的T-DNA端、(ii)包括篩選標(biāo)記、(iii)根據(jù)需要，具有基因表達(dá)供體DNA的啟動子和終止子。將所述載體轉(zhuǎn)移至含有Ti質(zhì)粒的根癌農(nóng)桿菌菌株中，該Ti質(zhì)粒含有vir基因。vir基因負(fù)責(zé)將DNA轉(zhuǎn)至布拉霉菌中。這個雙載體系統(tǒng)用于將DNA由農(nóng)桿菌轉(zhuǎn)移至布拉霉菌中。為此，農(nóng)桿菌首先在乙酰丁香酮存在條件下進(jìn)行孵育。乙酰丁香酮誘導(dǎo)vir基因。然后將三孢布拉霉的孢子與誘導(dǎo)的根癌農(nóng)桿菌細(xì)胞在含有乙酰丁香酮的培養(yǎng)基中共同孵育，隨后再轉(zhuǎn)移至能夠篩選轉(zhuǎn)化子的培養(yǎng)基中，即篩選經(jīng)遺傳修飾的布拉霉菌株。
術(shù)語“載體”本申請中指用于將外源DNA導(dǎo)入并根據(jù)需要在細(xì)胞中增殖所述外源DNA的DNA分子(也見R_mpp Lexikon Chemie-CDROM2.0版本，Stuttgart/New YorkGeorg Thieme Verlag 1999中“載體”)。術(shù)語“載體”這里也意指包括質(zhì)粒、粘粒等相同用途的分子。
“表達(dá)”本申請中指轉(zhuǎn)移從DNA或RNA到基因產(chǎn)物(這里優(yōu)選產(chǎn)生類胡蘿卜素和特別是葉黃素，特別優(yōu)選地是蝦青素或玉米黃素，以及八氫番茄紅素或紅木素的酶類)的遺傳信息，并也欲包括術(shù)語“過量表達(dá)”，指提高在未轉(zhuǎn)化細(xì)胞(野生型)也產(chǎn)生的基因產(chǎn)物的表達(dá)，使其增大產(chǎn)生或在整個細(xì)胞內(nèi)容物中形成一大部分。
遺傳修飾是指將遺傳信息導(dǎo)入受體生物以使所述信息以穩(wěn)定的方式表達(dá)并在細(xì)胞分化過程中傳遞。在本文中，同型核轉(zhuǎn)變是是產(chǎn)生僅僅含有一致細(xì)胞核的細(xì)胞，即細(xì)胞核具有同樣的遺傳信息內(nèi)容。
該同型核轉(zhuǎn)變只有在如果通過轉(zhuǎn)化導(dǎo)入的遺傳信息是隱性，即不表現(xiàn)其本身的時候才需要。然而，如果轉(zhuǎn)化導(dǎo)致顯性遺傳信息存在，即如果所述信息表現(xiàn)其本身，同型核轉(zhuǎn)變并不一定是必要的。
同型核轉(zhuǎn)變優(yōu)選地包含篩選單核孢子。三孢布拉霉孢子的一小部分本身就是單核的，所以這些孢子可以根據(jù)需要在特定標(biāo)記細(xì)胞核，例如染色后將其區(qū)分出來。這優(yōu)選使用基于單核細(xì)胞的低熒光的FACS(熒光激發(fā)細(xì)胞分選)方法進(jìn)行。
做為選擇地，同型核轉(zhuǎn)變可以首先通過減少細(xì)胞核的數(shù)量來進(jìn)行。為此，可以使用誘變劑，特別是N-甲基-N’-硝基亞硝基胍(MNNG)。高能量輻射如紫外輻射或X射線也可以用來減少核的數(shù)目。隨后可以用FACS法或隱性篩選標(biāo)記來進(jìn)行選擇。
篩選是指篩選細(xì)胞核含有相同遺傳信息的細(xì)胞，即具有相同的性質(zhì)如抗性或產(chǎn)生或提高一種產(chǎn)品的產(chǎn)量的細(xì)胞。除了FACS法，優(yōu)選5-碳-5-脫氮核黃素(DARF)和潮霉素(hyg)或5’-氟乳清酸(FOA)和尿嘧啶用于篩選。
轉(zhuǎn)化(i)中使用的質(zhì)?？梢匀藶樵O(shè)計，使得所述載體中包含的遺傳信息能整合到至少一個細(xì)胞的基因組中。就此而論，可以關(guān)閉細(xì)胞中的遺傳信息。這可以通過如刪除的方法直接進(jìn)行。然而，也可以將轉(zhuǎn)化(i)中使用的載體設(shè)計為所述載體中包含的遺傳信息在細(xì)胞中表達(dá)，即導(dǎo)入的遺傳信息并不在相應(yīng)的野生型中表達(dá)或通過所述轉(zhuǎn)化使其增高或過量表達(dá)以及其產(chǎn)物關(guān)閉基因。然而，導(dǎo)入的遺傳信息可以間接關(guān)閉細(xì)胞中的遺傳信息，如通過產(chǎn)生抑制劑的方法。
使用的載體包含產(chǎn)生類胡蘿卜素或其前體，特別是胡蘿卜素或葉黃素或其前體的遺傳信息或者部分的所述遺傳信息。使用的載體優(yōu)選含有產(chǎn)生蝦青素、玉米黃素、海膽酮、β-隱黃素、β-胡蘿卜素、金盞花黃質(zhì)(andonixanthin)、金盞花玉紅質(zhì)(adonirubine)、角黃素、3-羥海膽酮、3’-羥海膽酮、番茄紅素、葉黃素、六氫番茄紅素、紅木素或八氫番茄紅素的遺傳信息。特別優(yōu)選的載體含有產(chǎn)生紅木素、八氫番茄紅素、角黃素、蝦青素或玉米黃素的信息。
載體可以含有任何對布拉霉屬生物遺傳修飾的遺傳信息。
“遺傳信息”優(yōu)選地指導(dǎo)入布拉霉屬生物而導(dǎo)致布拉霉屬生物遺傳修飾的核酸，即，例如導(dǎo)致與起始生物相比增高或降低酶活性的核酸。
載體可以包含例如產(chǎn)生親脂性物質(zhì)如類胡蘿卜素和其前體、磷脂、甘油三酯、類固醇、臘類、脂溶性維生素、維生素原和輔因子的遺傳信息，或產(chǎn)生親水性物質(zhì)如蛋白質(zhì)、氨基酸、核苷酸和水溶性維生素、維生素原和輔因子的遺傳信息。
使用的載體優(yōu)選包含產(chǎn)生類胡蘿卜素或葉黃素或其前體的遺傳信息。
使用的載體優(yōu)選包含致使類胡蘿卜素生物合成酶定位至類胡蘿卜素生物合成發(fā)生的細(xì)胞小室中的遺傳信息。
特別優(yōu)選產(chǎn)生蝦青素、玉米黃素、海膽酮、β-隱黃素、金盞花黃質(zhì)、金盞花玉紅質(zhì)、角黃素、3-和3’-羥海膽酮、番茄紅素、葉黃素、β-胡蘿卜素、八氫番茄紅素和/或六氫番茄紅素的遺傳信息。非常特別優(yōu)選產(chǎn)生八氫番茄紅素、紅木素、番茄紅素、玉米黃素、角黃素和/或蝦青素的遺傳信息。
因此，本發(fā)明優(yōu)選的改變形式包括產(chǎn)生并培養(yǎng)具有增高的類胡蘿卜素生物合成中間體合成速率并從而具有增高的類胡蘿卜素生物合成終產(chǎn)物產(chǎn)量的生物。類胡蘿卜素生物合成中間體合成速率特別是通過提高3-羥基-3-甲基戊二酰基輔酶A還原酶(HMG-CoA還原酶)、異戊烯焦磷酸異構(gòu)酶和香葉基焦磷酸合成酶活性而得到提高的。
因此，本發(fā)明特別優(yōu)選的改變形式包括產(chǎn)生和培養(yǎng)具有與野生型相比提高了的HMG-CoA還原酶活性的生物。
HMG-CoA還原酶活性是指HMG-CoA還原酶(3-羥基-3-甲基戊二?；o酶A還原酶)的酶活性。
HMG-CoA還原酶是指具有將3-羥基-3-甲基戊二?；o酶A轉(zhuǎn)化為甲羥戊酸的酶活性的蛋白質(zhì)。
因此，HMG-CoA還原酶活性是指在特定時間內(nèi)轉(zhuǎn)化了的3-羥基-3-甲基戊二?；o酶A量或由HMG-CoA還原酶蛋白質(zhì)產(chǎn)生的甲羥戊酸量。
在提高了與野生型相比的HMG-CoA還原酶活性的情況下，HMG-CoA還原酶蛋白質(zhì)因此提高了在特定時間內(nèi)與野生型相比轉(zhuǎn)化3-羥基-3-甲基戊二?；o酶A的量或生成甲羥戊酸的量。
HMG-CoA還原酶活性優(yōu)選增高至少5％、更優(yōu)選至少20％、更優(yōu)選至少50％、更優(yōu)選至少100％、特別優(yōu)選至少300％、還更優(yōu)選至少500％，特別是至少600％的野生型HMG-CoA還原酶活性。
在優(yōu)選的實(shí)施方案中，通過提高編碼HMG-CoA還原酶核酸的基因表達(dá)使HMG-CoA還原酶活性與野生型相比得到提高。
在本發(fā)明方法特別優(yōu)選的實(shí)施方案中，通過將包含編碼HMG-CoA還原酶核酸的核酸構(gòu)建體導(dǎo)入生物體使得編碼HMG-CoA還原酶的核酸基因表達(dá)增高，所述生物的HMG-CoA還原酶的表達(dá)與野生型相比通常受到減少的調(diào)節(jié)。
與野生型相比減少的調(diào)節(jié)是指比上述野生型在表達(dá)或蛋白質(zhì)水平上，受到減少的調(diào)節(jié)優(yōu)選地不受調(diào)節(jié)。
減少的調(diào)節(jié)優(yōu)選地可以通過功能上與核酸構(gòu)建體中編碼序列相連的啟動子實(shí)現(xiàn)，且該啟動子與野生型啟動子相比常受到減少的調(diào)節(jié)。
例如，三孢布拉霉的ptef1和構(gòu)巢曲霉的pgpdA啟動子只受到減少的調(diào)節(jié)并因此是特別優(yōu)選的啟動子。
這些啟動子在三孢布拉霉中表現(xiàn)為幾乎組成型表達(dá)以至于通過類胡蘿卜素生物合成中間體進(jìn)行的轉(zhuǎn)錄調(diào)控不再發(fā)生。
在進(jìn)一步優(yōu)選的實(shí)施方案中，所述減少的調(diào)節(jié)可通過使用編碼HMG-CoA還原酶的核酸實(shí)現(xiàn)，HMG-CoA還原酶在所述生物中與所述生物固有的直向同源核酸相比受到減少的調(diào)節(jié)。
特別優(yōu)選的是用僅編碼HMG-CoA還原酶催化結(jié)構(gòu)域(截短的(t-)HMG-CoA還原酶)的核酸。負(fù)責(zé)調(diào)節(jié)的膜結(jié)構(gòu)域是不存在的。這樣使用的核酸受到減少的調(diào)節(jié)并因此造成HMG-CoA還原酶基因表達(dá)的增高。
在特別優(yōu)選的實(shí)施方案中，將含有序列SEQ ID.NO.75的核酸導(dǎo)入到三孢布拉霉中。
HMG-CoA還原酶和削減為催化域的t-HMG-CoA還原酶或編碼基因的進(jìn)一步的實(shí)例已經(jīng)例如易于從多種生物體中得到發(fā)現(xiàn)，這些生物的基因組序列是通過在數(shù)據(jù)庫中與SEQ ID.NO.75進(jìn)行同源對比而知道的。
而且，HMG-CoA還原酶和削減為催化域的t-HMG-CoA還原酶或編碼基因的進(jìn)一步的實(shí)例也已經(jīng)根據(jù)SEQ ID.NO.75從多種基因組序列未知的生物中通過雜交和PCR技術(shù)以本身已知的方式找到。
在特別優(yōu)選的實(shí)施方案中，所述減少的調(diào)節(jié)是通過使用編碼在所述生物中HMG-CoA還原酶表達(dá)與所述生物固有的直向同源核酸相比受到減少調(diào)節(jié)的HMG-CoA還原酶的核酸和在所述生物中與野生型啟動子相比受到減少調(diào)節(jié)的啟動子得到實(shí)現(xiàn)的。
因此，本發(fā)明優(yōu)選的改變形式包括轉(zhuǎn)化關(guān)閉八氫番茄紅素脫氫酶基因的表達(dá)從而使生物體產(chǎn)生的八氫番茄紅素得到分離。轉(zhuǎn)化(i)中使用的載體因此在本發(fā)明優(yōu)選的實(shí)施方案中包含編碼八氫番茄紅素脫氫酶基因片段的序列，特別是三孢布拉霉具有SEQ ID.NO.69的carB片段的序列。
因此，本發(fā)明優(yōu)選的改變形式包括通過轉(zhuǎn)化關(guān)閉番茄紅素環(huán)化酶基因表達(dá)，從而使生物產(chǎn)生的番茄紅素得到分離。所述轉(zhuǎn)化使用的載體因此在本發(fā)明優(yōu)選的實(shí)施方案中包含編碼番茄紅素環(huán)化酶基因片段的序列，特別是三孢布拉霉carR片段的序列。
在優(yōu)選的實(shí)施方案中，通過遺傳修飾的布拉霉屬生物與野生型相比引起羥化酶和/或酮酶活性使布拉霉屬生物能夠例如產(chǎn)生葉黃素，如角黃素、玉米黃素或蝦青素、紅木素或八氫番茄紅素。
因此，本發(fā)明進(jìn)一步優(yōu)選的改變形式為轉(zhuǎn)化(i)中使用的載體包含表達(dá)后顯示酮酶和/或羥化酶活性的遺傳信息，以使生物產(chǎn)生玉米黃素或蝦青素。
酮酶活性是指酮酶的酶活性。
酮酶是指具有在類胡蘿卜素的任選取代的β-紫羅酮環(huán)處導(dǎo)入酮基的酶活性的蛋白質(zhì)。
酮酶特別是指具有將β-胡蘿卜素轉(zhuǎn)化為角黃素的酶活性的蛋白質(zhì)。
因此，酮酶活性是指在特定時間內(nèi)通過酮酶蛋白質(zhì)轉(zhuǎn)化的β-胡蘿卜素量或產(chǎn)生的角黃素量。
根據(jù)本發(fā)明，術(shù)語“野生型”是指相應(yīng)的未經(jīng)遺傳修飾的布拉霉屬起始生物。
術(shù)語“生物”根據(jù)上下文可以指布拉霉屬起始生物(野生型)或布拉霉屬的經(jīng)遺傳修飾生物或二者兼指。
優(yōu)選地，引起酮酶活性和羥化酶活性的“野生型”在每種情況下是指參照生物。
這種布拉霉屬的參照生物是三孢布拉霉ATCC14271或ATCC14272，二者的區(qū)別僅在于交配型不同。
根據(jù)本發(fā)明，布拉霉屬經(jīng)遺傳修飾的生物和野生型或參照生物的酮酶活性優(yōu)選地在以下條件下測定布拉霉屬生物的酮酶活性是根據(jù)Franzer等的方法確定的(J.Biol.Chem.272(10)6128-6135，1997)。提取物中的酮酶活性是通過用β-胡蘿卜素和角黃素底物在脂(大豆卵磷脂)類和去污劑(膽酸鈉)存在的情況下得到確定的。酮酶分析法中底物對產(chǎn)物比率是通過HPLC得到確認(rèn)的。
在該優(yōu)選的實(shí)施方案中，根據(jù)本發(fā)明的布拉霉屬經(jīng)遺傳修飾生物和未經(jīng)遺傳修飾的野生型相比，前者具有酮酶活性并因此優(yōu)選地能遺傳改造地表達(dá)酮酶。
在進(jìn)一步優(yōu)選的實(shí)施方案中，布拉霉屬生物中的酮酶活性是通過使編碼酮酶的核酸進(jìn)行表達(dá)來實(shí)現(xiàn)的。
在該優(yōu)選的實(shí)施方案中，編碼酮酶的核酸的表達(dá)優(yōu)選地是通過將編碼酮酶的核酸導(dǎo)入布拉霉屬起始生物實(shí)現(xiàn)的。
為了這一目的，原則上可利用任一酮酶基因，例如任一編碼酮酶的核酸。
在本說明書中提到的任何一種核酸可以是例如RNA、DNA或cDNA序列。
在真核來源的基因組酮酶序列的情形下，其序列含有內(nèi)含子，如果布拉霉屬生物宿主不能表達(dá)相應(yīng)的酮酶，優(yōu)選使用已經(jīng)加工過的核酸序列如相應(yīng)的cDNA。
可用于本發(fā)明方法的編碼一種酮酶和相應(yīng)酮酶的核酸的例子是以下來源的序列雨生紅球藻(Haematoccus pluvialis)，特別是來源于雨生紅球藻Flotow em.Wille(登錄號X86782；核酸SEQ ID NO11，蛋白質(zhì)SEQ IDNO12)，雨生紅球藻，NIES-144(登錄號D45881；核酸SEQ ID NO13，蛋白質(zhì)SEQ ID NO14)、橙黃農(nóng)桿菌(Agrobacterium aurantiacum)(登錄號D58420；核酸SEQ ID NO15，蛋白質(zhì)SEQ ID NO16)、產(chǎn)堿菌(Alicaligenes spec.)(登錄號D58422；核酸SEQ ID NO17，蛋白質(zhì)SEQ ID NO18)、Paracoccus marcusii(登錄號Y15112；核酸SEQ ID NO19，蛋白質(zhì)SEQ ID NO20)。
集胞藻(Synechocystis)PC6803株(登錄號NP442491；核酸SEQ IDNO21，蛋白質(zhì)SEQ ID NO22)。
慢生根瘤菌(Bradyrhizobium sp.)(登錄號AF218415；核酸SEQID NO23，蛋白質(zhì)SEQ ID NO24)。
念珠藻PCC7120株(登錄號AP003592，BAB74888；核酸SEQ IDNO25，蛋白質(zhì)SEQ ID NO26)、點(diǎn)形念珠藻(Nostoc punctiforme)ATTC 29133，核酸登錄號NZ_AABC01000195，堿基對55,604至55,392(SEQ ID NO27)；蛋白質(zhì)登錄號.ZP_00111258(SEQ ID NO28)(注釋為推定的蛋白質(zhì))、點(diǎn)形念珠藻ATTC29133，核酸登錄號NZ_AABC01000196，堿基對140,571至139,810(SEQ ID NO29)，蛋白質(zhì)(SEQ ID NO30)(未注釋)。
可用于本發(fā)明方法的酮酶和酮酶基因的其它天然例子易于例如通過將數(shù)據(jù)庫中的氨基酸序列或相應(yīng)的反向翻譯的核酸序列與上述序列特別是序列SEQ ID NO12、26和/或33進(jìn)行序列對比而在基因組序列已知的多種生物中找到。
此外，酮酶和酮酶基因的其它天然例子可以通過雜交的技術(shù)從上述核酸序列出發(fā)、特別是從SEQ ID NO12、26和/或30出發(fā)，易于自基因組序列未知的多種生物中找到。
雜交可在溫和(低嚴(yán)格)的條件下進(jìn)行，或優(yōu)選地，在嚴(yán)格(高嚴(yán)格性)的條件下進(jìn)行。
這些類型的雜交條件在例如Sambrook，J.，F(xiàn)ritsch，E.F.，Maniatis，T.，Molecular Cloning(A Laboratory Manual)，2nd edition，Cold SpringHarbor Laboratory Press，1989，9.31-9.57頁或Current Protocols inMolecular Biology，John Wiley & Sons，N.Y.(1989)，6.3.1-6.3.6.中描述。
例如，洗滌步驟中的條件可以從界定為低嚴(yán)格條件(2X SSC在50℃)和高嚴(yán)格條件(0.2X SSC在50℃，優(yōu)選地在65℃)(20X SSC0.3M檸檬酸鈉，3M氯化鈉，pH7.0)之間的范圍進(jìn)行選擇。
另外的可能是在洗滌步驟中將溫度從室溫22℃的溫和條件提高到65℃的嚴(yán)格條件。
鹽濃度和溫度這兩個參數(shù)可以同時改變，而且也可保持兩個參數(shù)中的一個恒定并只改變另外一個。還可以在雜交過程使用例如甲酰胺或SDS的變性劑。在50％甲酰胺存在時，雜交優(yōu)選地在42℃進(jìn)行。
下面給出了一些雜交和洗滌步驟的例子(1)雜交條件如(i)4X SSC，于65℃，或(ii)6X SSC，于45℃，或(iii)6X SSC，于68℃，100mg/ml變性的魚精DNA，或(iv)6X SSC，0.5％ SDS，100mg/ml變性的鮭精DNA片段，于68℃，或(v)6X SSC，0.5％ SDS，100mg/ml變性的鮭精DNA片段，50％甲酰胺，于42℃，或(vi)50％甲酰胺，4X SSC，于42℃，或(vii)50％(體積/體積)甲酰胺，0.1％牛血清白蛋白，0.1％菲克，0.1％聚乙烯吡咯烷酮，50mM磷酸鈉緩沖液pH6.5，750mM氯化鈉，75mM檸檬酸鈉，于42℃，或(viii)2X或4X SSC于50℃(溫和條件)，或(ix)30到40％甲酰胺，2X或4X SSC于42℃(溫和條件)。
(2)每十分種洗滌步驟用如，(i)0.015M氯化鈉/0.0015M檸檬酸鈉/0.1％SDS于50℃，或(ii)0.1X SSC于65℃，或
(iii)0.1X SSC，0.5％SDS于68℃，或(iv)0.1X SSC，0.5％SDS，50％甲酰胺于42℃，或(v)0.2X SSC，0.1％SDS于42℃，或(vi)2X SSC于65℃(溫和條件)。
在本發(fā)明布拉霉屬經(jīng)遺傳修飾生物的優(yōu)選實(shí)施方案中，導(dǎo)入編碼包含氨基酸序列SEQ ID NO12的蛋白質(zhì)的核酸，或?qū)刖幋a包含通過替換、插入或刪除SEQ ID NO12中的氨基酸而衍生的、并與具有酮酶特性的序列SEQ ID NO12在氨基酸水平至少有20％的同一性、優(yōu)選地至少30％、40％、50％、60％，優(yōu)選地至少70％、80％，特別優(yōu)選至少90％，特別是91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的序列的核酸。
因此，酮酶序列可能是通過上述與其他生物的序列比較同一性而發(fā)現(xiàn)的天然序列，或從SEQ ID NO12的序列出發(fā)經(jīng)過人為的改變?nèi)缣鎿Q、插入或刪除氨基酸而得到的經(jīng)修飾人工序列。
在另一優(yōu)選的本發(fā)明方法的實(shí)施方案中，涉及導(dǎo)入編碼包含氨基酸序列SEQ ID NO26的蛋白質(zhì)的核酸，或?qū)刖幋a包含通過替換、插入或刪除SEQ ID NO26中的氨基酸而衍生的、并與具有酮酶特性的序列SEQID NO26在氨基酸水平至少有20％的同一性、優(yōu)選地至少30％、40％、50％、60％，優(yōu)選地至少70％、80％，特別優(yōu)選至少90％，特別是91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的序列的核酸。
因此，酮酶序列可能是通過上述與其他生物的序列比較同一性而發(fā)現(xiàn)的天然序列，或從SEQ ID NO26的序列出發(fā)經(jīng)過人為的改變?nèi)缣鎿Q、插入或刪除氨基酸而得到的經(jīng)修飾人工序列。
在另一優(yōu)選的本發(fā)明方法的實(shí)施方案中，涉及導(dǎo)入編碼包含氨基酸序列SEQ ID NO30的蛋白質(zhì)的核酸，或?qū)刖幋a包含通過替換、插入或刪除SEQ ID NO30中的氨基酸而衍生的、并與具有酮酶特性的序列SEQID NO30在氨基酸水平至少有20％的同一性、優(yōu)選地至少30％、40％、50％、60％，優(yōu)選地至少70％、80％，特別優(yōu)選至少90％，特別是91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的序列的核酸。
因此，酮酶序列可能是通過上述與其他生物的序列比較同一性而發(fā)現(xiàn)的天然序列，或從SEQ ID NO30的序列出發(fā)經(jīng)過人為的改變?nèi)缣鎿Q、插入或刪除氨基酸而得到的經(jīng)修飾人工序列。
本說明書中術(shù)語“替換”是替換一個或多個氨基酸為一個或多個氨基酸。優(yōu)選地進(jìn)行用與原始氨基酸性質(zhì)相似的氨基酸代替的“保守”替換，如天冬氨酸替換谷氨酸、天冬酰氨替換谷氨酰氨、異亮氨酸替換纈氨酸、異亮氨酸替換亮氨酸、蘇氨酸替換絲氨酸。
刪除是指用直接的鍵代替氨基酸。優(yōu)選的刪除位置是多肽的末端和蛋白質(zhì)結(jié)構(gòu)域之間的聯(lián)接。
插入是指將氨基酸插入多肽鏈中，形式上為用一個或多個氨基酸代替直接的鍵。
兩個蛋白質(zhì)之間的同一性是指每個蛋白質(zhì)的全長氨基酸的同一性，特別是借助來自威斯康星州麥迪遜DNASTAR公司的Lasergene軟件用clustal方法(Higgins DG，Sharp PM.快速敏感的微機(jī)多重序列比對，Comput Appl.Biosci.1989 Apr；5(2)151-1)對比計算出的同一性。該方法設(shè)置以下參數(shù)多重比對參數(shù)缺口罰分 10缺口長度罰分 10成對比對參數(shù)K-tuple1缺口罰分 3窗口 5保留對角線(Diagonals saved)5因此，某一蛋白質(zhì)與SEQ ID NO12或26或30的序列在氨基酸水平有至少20％的同一性是指在將其序列與SEQ ID NO12或26或30的序列，特別是用具有上述參數(shù)設(shè)置的上述程序算法進(jìn)行對比時，具有至少20％的同一性，優(yōu)選地30％、40％、50％，特別優(yōu)選地60％、70％、80％，特別是85％、90％、95％的同一性。
合適的核酸序列可以通過例如將多肽序列根據(jù)遺傳密碼反向翻譯而獲得。
為了這一目的優(yōu)選使用的密碼子是根據(jù)布拉霉特異的密碼子選擇而頻繁使用的那些密碼子。這些密碼子選擇可以很容易地通過計算機(jī)分析其他已知布拉霉屬生物基因而獲得。
在特別優(yōu)選的實(shí)施方案中，將包含SEQ ID NO11序列的核酸導(dǎo)入到所述屬的生物中。
在特別優(yōu)選的實(shí)施方案中，將包含SEQ ID NO25序列的核酸導(dǎo)入到所述屬的生物中。
在特別優(yōu)選的實(shí)施方案中，將包含SEQ ID NO29序列的核酸導(dǎo)入到所述屬的生物中。
此外，所有上述酮酶基因能夠通過化學(xué)合成法以本身已知的方式從核苷酸結(jié)構(gòu)單元產(chǎn)生，例如通過雙螺旋各個重疊互補(bǔ)核酸結(jié)構(gòu)單元的片段縮合。例如，通過亞磷酰胺法(Voet，Voet，第二版，Wiley Press New York，896-897頁)以已知的方式進(jìn)行寡核苷酸的化學(xué)合成。合成寡核苷酸的添加和利用DNA聚合酶克列諾片段進(jìn)行缺口補(bǔ)平和連接反應(yīng)，并且常規(guī)的克隆方法描述于Sambrook等(1989)，Molecular cloningA laboratorymanual，冷泉港實(shí)驗室出版社。
在本發(fā)明的一個實(shí)施方案中轉(zhuǎn)化(i)中使用的載體因此優(yōu)選地包含編碼酮酶，特別是SEQ ID NO72的點(diǎn)形念珠藻酮酶的序列。
羥化酶活性是指羥化酶的酶活性。
羥化酶是指具有將羥基導(dǎo)入類胡蘿卜素的任選取代的β-紫羅酮環(huán)的酶活性的蛋白質(zhì)。
特別地，羥化酶是指具有將β-胡蘿卜素轉(zhuǎn)化為玉米黃素或?qū)⒔屈S素轉(zhuǎn)化為蝦青素的酶活性的蛋白質(zhì)。
因此，羥化酶活性是指在特定時間內(nèi)羥化酶蛋白質(zhì)轉(zhuǎn)化的β-胡蘿卜素或角黃素的量，或產(chǎn)生的玉米黃素或蝦青素的量。
因而，當(dāng)羥化酶活性與野生型相比得到提高是指，在特定時間內(nèi)羥化酶蛋白質(zhì)與野生型相比轉(zhuǎn)化的β-胡蘿卜素或角黃素的量或者生成的玉米黃素或蝦青素的量得到提高。
羥化酶活性優(yōu)選提高至少5％、進(jìn)一步優(yōu)選至少20％、進(jìn)一步優(yōu)選至少50％、進(jìn)一步優(yōu)選至少100％，更加優(yōu)選至少300％、還更加優(yōu)選至少500％、特別是至少600％的野生型羥化酶活性。
本發(fā)明的經(jīng)遺傳修飾生物和野生型及參照生物的羥化酶活性優(yōu)選在以下條件下確定在體外羥化酶活性可通過Bouvier等的方法(Biochim.Biophys.Acta1391(1998)，320-328)得到確定。將鐵氧還蛋白、鐵氧還蛋白-NADP氧化還原酶、過氧化氫酶、NADPH和β-胡蘿卜素，與單-或二-半乳糖苷甘油一起，加入到一定量的生物提取物中。
特別優(yōu)選地，按照Bouvier、Keller、d’Harlingue和Camara(葉黃素的生物合成來自辣椒(Capsicum annuum L.)的類胡蘿卜素羥化酶的分子和功能特性(Biochim.Biophys.Acta 1391(1998)，320-328)方法在以下條件下測定羥化酶活性體外測定在0.250ml體積中進(jìn)行。測定混合物含有50mM磷酸鉀(pH 7.6)、0.025mg菠菜鐵氧還蛋白、0.5單位鐵氧還蛋白-NADP+菠菜氧化還原酶、0.25mM NADPH、0.010mgβ-胡蘿卜素(乳化于0.1mg吐溫80)、0.05mM單-和二-半乳糖苷甘油混合物(1∶1)、1單位過氧化氫酶、200單-和二-半乳糖苷甘油(1∶1)、0.2mg牛血清白蛋白和不同體積的生物提取物。將反應(yīng)混合物在30℃孵育2小時。用有機(jī)溶劑諸如丙酮或氯仿/甲醇(2∶1)，提取反應(yīng)產(chǎn)物并且用HPLC測定。
特別優(yōu)選地，按照Bouvier、d’Harlingue和Camara(類胡蘿卜素環(huán)化酶抑制的分子分析；Arch.Biochem.Biophys.346(1)(1997)53-64)方法在以下條件下測定羥化酶活性體外測定在250μl體積中進(jìn)行。測定混合物含有50mM磷酸鉀(pH 7.6)、不同量的生物提取物、20nM番茄紅素、250μg紅辣椒色質(zhì)體基質(zhì)蛋白質(zhì)、0.2mM NADP+、0.2mM NADPH和1mM ATP。當(dāng)加入孵育介質(zhì)之前，立即將NADP/NADPH和ATP與1mg吐溫80一起溶于10ml乙醇中。30℃反應(yīng)60分鐘后，加入氯仿/甲醇(2∶1)終止反應(yīng)。用HPLC方法分析所提取的反應(yīng)產(chǎn)物。
另一種利用放射性底物的測定法描述于Fraser和Sandmann(Biochem.Biophys.Res.Comm.185(1)(1992)9-15)。
羥化酶活性可通過多種途徑得到提高，例如在表達(dá)和蛋白水平關(guān)閉抑制性調(diào)控機(jī)制或提高與野生型相比的編碼羥化酶的核酸的基因表達(dá)。
編碼羥化酶的核酸的基因表達(dá)同樣可通過多種途徑與野生型相比得到提高，例如通過活化因子誘導(dǎo)羥化酶基因或?qū)胍粋€或多個羥化酶基因拷貝，即將至少一個編碼羥化酶的核酸導(dǎo)入布拉霉屬生物。
在優(yōu)選的實(shí)施方案中，編碼羥化酶核酸的基因表達(dá)通過將至少一個編碼羥化酶基因的核酸導(dǎo)入布拉霉屬生物中得到提高。
為了這一目的，原則上可利用任一羥化酶基因，即任何編碼羥化酶的核酸和任何編碼β-環(huán)化酶的核酸。
在含有內(nèi)含子的真核來源基因組羥化酶序列情況下，如果宿主生物沒有能力或不能使其表達(dá)相應(yīng)的羥化酶，優(yōu)選使用已經(jīng)得到加工的核酸序列，如相應(yīng)的cDNA。
羥化酶基因的一個實(shí)例是登錄號為AX038729的編碼雨生紅球藻羥化酶的核酸(WO 0061764；核酸SEQ ID NO31，蛋白質(zhì)SEQ ID NO32)、嗜夏孢歐文氏菌(Erwinia uredovora)20D3羥化酶(ATCC19321，登錄號D90087；核酸SEQ ID NO33，蛋白質(zhì)SEQ ID NO34)、或由序列SEQID NO76編碼的嗜熱棲熱菌(Thermus thermophilus)羥化酶(DE10234126.5)。
也可使用具有以下登錄號的羥化酶|emb|CAB55626.1，CAA70427.1，CAA70888.1，CAB55625.1，AF499108_1，AF315289_1，AF296158_1，AAC49443.1，NP_194300.1，NP_200070.1，AAG10430.1，CAC06712.1，AAM88619.1，CAC95130.1，AAL80006.1，AF162276_1，AAO53295.1，AAN85601.1，CRTZ_ERWHE，CRTZ_PANAN，BAB79605.1，CRTZ_ALCSP，CRTZ_AGRAU，CAB56060.1，ZP_00094836.1，AAC44852.1，BAC77670.1，NP_745389.1，NP_344225.1，NP_849490.1，ZP_00087019.1，NP_503072.1，NP_852012.1，NP_115929.1，ZP_00013255.1因此，在該優(yōu)選的實(shí)施方案中，與其野生型相比，在本發(fā)明優(yōu)選的布拉霉屬轉(zhuǎn)基因生物中，至少額外存在一個羥化酶基因。
在本優(yōu)選的實(shí)施方案中，經(jīng)遺傳修飾的生物具有例如至少一個編碼羥化酶的外源核酸或至少兩個編碼羥化酶的內(nèi)源核酸。
在上述優(yōu)選的實(shí)施方案中，優(yōu)選使用這樣的羥化酶基因的核酸，其編碼包含氨基酸序列SEQ ID NO32、34的蛋白質(zhì)，或由SEQ ID NO76的序列編碼，或編碼通過替換、插入或刪除SEQ ID NO32、34中的氨基酸而衍生的、并與序列SEQ ID NO32、34在氨基酸水平至少有30％的同一性、優(yōu)選地至少30％、優(yōu)選地至少50％、更優(yōu)選地至少70％、仍更優(yōu)選地至少80％、更優(yōu)選地至少90％、特別是91％、92％、93％、94％、95％、96％、97％、98％、99％同一性的、具有羥化酶酶學(xué)特性的序列，或由與SEQ ID NO76序列同源的具有羥化酶酶學(xué)特性的序列編碼。
例如通過將來自數(shù)據(jù)庫的氨基酸序列或相應(yīng)的反向翻譯核酸序列與SEQ ID.NO31、33或76進(jìn)行同源性比較，可以容易地從多種基因組序列已知的生物發(fā)現(xiàn)其它羥化酶和羥化酶基因的實(shí)例。
例如，如上所述，通過雜交和PCR技術(shù)以本身已知的方式，例如自序列SEQ ID NO31、33或76起始容易地從來自其基因組序列未知的多種生物發(fā)現(xiàn)其它羥化酶和羥化酶基因的實(shí)例。
在另一個特別優(yōu)選的實(shí)施方案中，將編碼包含羥化酶氨基酸序列SEQID NO32、34的蛋白質(zhì)的核酸，或由序列SEQ ID NO76編碼的核酸導(dǎo)入生物以增加羥化酶活性。
例如，按照遺傳密碼通過多肽序列的反向翻譯可獲得適當(dāng)?shù)暮怂嵝蛄小?br> 對于這方面，優(yōu)選地使用那些依照生物特異的密碼子選擇頻繁使用的密碼子。基于對所討論生物的其它已知基因的計算機(jī)評估可以快速確定密碼子選擇。
在特別優(yōu)選的實(shí)施方案中，將含有SEQ ID NO31、33或76序列的核酸導(dǎo)入生物中。
所有上述羥化酶基因還可通過化學(xué)合成法以本身已知的方式從核苷酸結(jié)構(gòu)單元產(chǎn)生，例如通過雙螺旋各個重疊互補(bǔ)核酸結(jié)構(gòu)單元的片段縮合。例如，通過亞磷酰胺法(Voet，Voet，第二版，Wiley Press New York，896-897頁)以已知的方式進(jìn)行寡核苷酸的化學(xué)合成。合成寡核苷酸的添加和利用DNA聚合酶克列諾片段進(jìn)行缺口補(bǔ)平和連接反應(yīng)以及常規(guī)的克隆方法描述于Sambrook等(1989)，Molecular cloningA laboratory manual，冷泉港實(shí)驗室出版社。
在本發(fā)明的進(jìn)一步實(shí)施方案中轉(zhuǎn)化(i)使用的載體因此優(yōu)選地包含編碼羥化酶特別是編碼點(diǎn)形念珠藻羥化酶的序列SEQ ID NO70或編碼嗜夏孢歐文氏菌羥化酶的SEQ ID NO71或編碼嗜熱棲熱菌羥化酶的序列SEQ ID NO76。
優(yōu)選關(guān)閉八氫番茄紅素脫氫酶基因的轉(zhuǎn)化。
轉(zhuǎn)化(i)使用的載體也優(yōu)選包含調(diào)控和支持表達(dá)的區(qū)域，特別是啟動子和終止子。
轉(zhuǎn)化(i)使用的載體優(yōu)選地包括已經(jīng)在布拉霉屬轉(zhuǎn)化中特別成功地得到證實(shí)的gpd和/或ptef1啟動子和/或trpC終止子。使用技術(shù)人員熟悉的“反向重復(fù)序列”(IR，R_mpp Lexikon der Biotechnologie 1992，ThiemeVerlag Stuttgart，407頁，″反向重復(fù)序列”)來控制表達(dá)和翻譯也在本發(fā)明范疇內(nèi)。
載體中使用的gpd啟動子有利地具有序列SEQ ID NO1。載體中使用的trpC終止子有利地具有序列SEQ ID NO2。載體中使用的ptef1啟動子有利地具有序列SEQ ID NO35。
優(yōu)選使用特別是來自構(gòu)巢曲霉的gpd啟動子和trpC終止子和來自三孢布拉霉的ptef1啟動子。
轉(zhuǎn)化(i)使用的載體特別包含抗性基因。后者優(yōu)選潮霉素抗性基因(hph)，特別是大腸桿菌來源的潮霉素抗性基因。該抗性基因已被證實(shí)特別適合用于檢測轉(zhuǎn)化子并篩選細(xì)胞。
用于hph的啟動子優(yōu)選p-gpdA，為構(gòu)巢曲霉編碼的甘油醛-3-磷酸脫氫酶的啟動子。用于hph的終止子優(yōu)選t-trpC，為構(gòu)巢曲霉編碼鄰氨基苯甲酸合成酶組分的trpC基因的終止子。
pBinAHyg載體的衍生物被證實(shí)為特別適合的載體。用于轉(zhuǎn)化的載體因此優(yōu)選包含SEQ ID NO3。在載體上要根據(jù)想得到的類胡蘿卜素或其前體，如上所述，加入編碼羥化酶、酮酶、八氫番茄紅素脫氫酶等的序列。在一個本發(fā)明實(shí)施方案中，載體因此包含編碼所述八氫番茄紅素脫氫酶的序列SEQ ID NO69。在本發(fā)明進(jìn)一步的實(shí)施方案中，載體還包含編碼酮酶的序列SEQ ID NO72。在本發(fā)明進(jìn)一步的實(shí)施方案中，載體還包含編碼羥化酶的序列SEQ ID NO70或71或76。上述序列的相應(yīng)組合也在本發(fā)明的范疇內(nèi)。因此，在一實(shí)施方案中，載體不僅含有編碼酮酶的序列SEQID NO72，還含有編碼羥化酶的序列SEQ ID NO70或71或76，并因此能產(chǎn)生蝦青素。
特別地，在本發(fā)明范疇內(nèi)可以使用包含選自序列SEQ ID NO37到51和62的載體。
經(jīng)遺傳修飾的生物可以用來產(chǎn)生類胡蘿卜素、葉黃素或其前體，特別是紅木素、八氫番茄紅素、蝦青素、玉米黃素和角黃素。也可以通過導(dǎo)入適合的遺傳信息使特定的遺傳修飾細(xì)胞或由其形成的菌絲體產(chǎn)生在野生型中不能天然形成的新類胡蘿卜素并隨后得到分離。
篩選以后，培養(yǎng)經(jīng)遺傳修飾的細(xì)胞使其能供應(yīng)類胡蘿卜素或其前體。
優(yōu)選使用特定遺傳修飾的細(xì)胞或由其形成的菌絲體獲得類胡蘿卜素或其前體。
培養(yǎng)生物沒有特別的要求。特別是當(dāng)使用三孢布拉霉時，有利地，將相反的交配型共同培養(yǎng)，因為這導(dǎo)致更好的生長和生產(chǎn)。
如果遺傳修飾只在一種交配型細(xì)胞((+)或(-)三孢布拉霉)中實(shí)施了，將相應(yīng)的另外的未修飾交配型也加入培養(yǎng)基，因為第二種未修飾的交配型釋放的物質(zhì)(如三孢酸)可能使得獲得好的類胡蘿卜素或其前體產(chǎn)量。然而，有利地，在兩種交配型細(xì)胞中實(shí)施遺傳修飾，再將其共同培養(yǎng)，從而得到特別好的生長和類胡蘿卜素或其前體產(chǎn)量。
三孢酸是如布拉霉屬的毛霉真菌的性激素，它刺激接合子梗的形成和β-胡蘿卜素的產(chǎn)生(van den Ende 1968，J.Bacteriol.961298-1303，Austin等，1969，Nature 2231178-1179，Reschke Tetrahedron Lett.293435-3439，van den Ende 1970，J.Bacteriol.101423-428)。
可以使用技術(shù)人員熟悉的任何培養(yǎng)基，只要其適合培養(yǎng)所使用的生物和類胡蘿卜素的生產(chǎn)。特別地，當(dāng)使用“GMO”(遺傳修飾的生物)時，沒有必要使用類胡蘿卜素生物合成抑制劑。使用的培養(yǎng)基優(yōu)選地包括如一種或多種碳源、一種或多種氮源、礦鹽和硫胺素的添加劑。優(yōu)選使用的添加劑如WO 03/038064 A2，第4頁，第30行到第5頁，第7行所公開。特別優(yōu)選的碳源是葡萄糖且特別優(yōu)選的氮源是天冬酰胺、植物或動物提取物如棉籽油、大豆油、棉籽粉或酵母提取物。
培養(yǎng)可以在有氧或厭氧條件下進(jìn)行。如DE 101 30 323中所公開，也可進(jìn)行先有氧后厭氧的混合培養(yǎng)。在該情況下，要設(shè)置不同溫度和濕度以使生長最佳。培養(yǎng)溫度優(yōu)選約20和約34℃之間，特別是約26和約28℃之間。而且，可以連續(xù)或分批進(jìn)行培養(yǎng)。
優(yōu)選地在固體含量為約1和約20％之間，優(yōu)選3和15％之間并特別優(yōu)選4和11％之間進(jìn)行培養(yǎng)。特別重要的是培養(yǎng)肉湯保持可抽吸以便保持隨后加工步驟可行。如果固體含量過低，還需要復(fù)雜的濃縮或干燥步驟。
培養(yǎng)或發(fā)酵可以在常規(guī)的設(shè)備中進(jìn)行。這包括適用于每一情況下所用微生物和其產(chǎn)物的所用設(shè)備，特別是在R_mpp Lexikon Biotechnologie(1992 Georg Thieme Verlag，Stuttgart)一書123-126頁中關(guān)鍵詞“生物反應(yīng)器”下所述的設(shè)備。特別優(yōu)選使用具有多種內(nèi)部裝置、多種類型的起泡柱等的攪拌罐反應(yīng)器。
由本發(fā)明方法提供的類胡蘿卜素或其前體，特別是紅木素、八氫番茄紅素或葉黃素，特別優(yōu)選地蝦青素或玉米黃素特別適合產(chǎn)生飼料添加劑、食品和食品添加劑、化妝品、藥物或皮膚病學(xué)制劑。
由經(jīng)遺傳修飾細(xì)胞產(chǎn)生的類胡蘿卜素或由經(jīng)遺傳修飾細(xì)胞產(chǎn)生的類胡蘿卜素前體是根據(jù)a)或b)兩種方式，也優(yōu)選a)和b)兩種方式聯(lián)合從經(jīng)遺傳修飾微生物的培養(yǎng)物中制備的；aI)去除生物量，IA)用類胡蘿卜素不可溶的溶劑特別是水任選地洗滌生物量，IB)生物量的滅菌和細(xì)胞破裂，IC)任選地干燥和/或均相分布，并II)從破壞的生物量中通過類胡蘿卜素可溶的溶劑部分提取類胡蘿卜素并從所述生物量中分離所述溶劑，IIA)1)從含有類胡蘿卜素的生物量中去除殘余的溶劑，2)任選地均勻懸浮生物量，并使生物量固體含量＞2％并＜50％，并3)干燥生物量或重懸，用于產(chǎn)生食品，IIB)1)從使用的溶劑中結(jié)晶類胡蘿卜素并特別地用過濾法分離類胡蘿卜素晶體；或b)I)均勻懸浮培養(yǎng)肉湯的固體，并IIA)對于培養(yǎng)肉湯的固體含量＞2％1)任選地將培養(yǎng)肉湯濃縮至固體含量＜50％，并2)將培養(yǎng)肉湯干燥以產(chǎn)生食品，或IIB)對于培養(yǎng)肉湯的固體含量＜2％
1)將培養(yǎng)肉湯濃縮至固體含量＞2％且＜50％，并2)將懸液干燥以產(chǎn)生食品，或IIC)不依賴于培養(yǎng)肉湯的固體含量，1)去除生物量，2)任選地用類胡蘿卜素不可溶的溶劑特別是水洗滌生物量，3)滅菌并破壞細(xì)胞，4)任選地干燥和/或均勻分布，5)用類胡蘿卜素可溶的溶劑從生物量中部分提取類胡蘿卜素，5a)從含有類胡蘿卜素的溶劑中去除含有類胡蘿卜素的生物量，5b)從生物量中去除殘余的溶劑，并5c)干燥生物量以產(chǎn)生食品，6)從5a)使用的溶劑中結(jié)晶類胡蘿卜素并特別地通過過濾分離類胡蘿卜素晶體。
根據(jù)用遺傳修飾細(xì)胞產(chǎn)生類胡蘿卜素和用遺傳修飾細(xì)胞產(chǎn)生類胡蘿卜素前體的本發(fā)明，根據(jù)a)或b)兩種方式從經(jīng)遺傳修飾的微生物的培養(yǎng)物中的制備方法使得同時可以產(chǎn)生兩種產(chǎn)品。
根據(jù)本發(fā)明通過聯(lián)合產(chǎn)生兩種產(chǎn)品，特別是根據(jù)a)方式制備的，即至少一種類胡蘿卜素和含有類胡蘿卜素的食品，不需要從生物量中完全提取類胡蘿卜素，所以所述提取不是很復(fù)雜。除了完全利用外，類胡蘿卜素僅需要部分提取，并且沒有產(chǎn)品損失。這需要較少量的溶劑，并伴有更少量的其再用方法。而且，很大程度上避免了廢品，因為生物量最終不變成廢物而是加工成進(jìn)一步的具有高價值的食品。作為結(jié)果，由于利用了協(xié)同作用，該方法變得不再昂貴。
因此，根據(jù)本發(fā)明方法由b)方式制備的食品包括生產(chǎn)之后不需要再添加大量類胡蘿卜素的食品。而且，由于所述食品除了至少一種類胡蘿卜素，也含有三孢布拉霉，其營養(yǎng)物含量有所提高。根據(jù)優(yōu)選的備選方案IA和IB，營養(yǎng)物含量提高特別多，因為它除了至少一種類胡蘿卜素和三孢布拉霉，另外還包括發(fā)酵的全部培養(yǎng)基組分。而且，該過程不需要任何額外的復(fù)雜加工和制備步驟；確切地說，已勻漿和根據(jù)需要已脫水的含有三孢布拉霉的培養(yǎng)肉湯可以直接干燥產(chǎn)生食品。結(jié)果，除了備選的IIB中的水介質(zhì)外，事實(shí)上沒有廢品，而該水介質(zhì)可以在純化車間很容易得到純化。另外，所有的三種備選方案利用了全部產(chǎn)生的類胡蘿卜素并沒有或僅有少量的損失，因為，根據(jù)IIA和IIB，不需要進(jìn)行造成大量損失的分離或加工步驟。在可選的IIIC，產(chǎn)生的全部類胡蘿卜素量同樣也得到?jīng)]有損失或只有少量損失的利用，因為生物量中的一部分經(jīng)加工產(chǎn)生食品而其余的部分經(jīng)提取獲得純類胡蘿卜素。根據(jù)本發(fā)明聯(lián)合由IIC產(chǎn)生的兩種產(chǎn)品，即含有類胡蘿卜素的食品和類胡蘿卜素本身，導(dǎo)致了基本上沒有廢品產(chǎn)生的優(yōu)點(diǎn)以及從生物量中完全提取類胡蘿卜素是不必要的，所以通常復(fù)雜的提取就不再復(fù)雜。除了完全利用外，有價值的類胡蘿卜素僅需要部分提取，不招致產(chǎn)品損失。
這需要少量的溶劑，伴有更少的溶劑再利用方法。而且，大量避免了廢品，因為生物量最終不變成廢物而是進(jìn)一步加工成高價值的食品。結(jié)果，由于利用的協(xié)同作用，該方法變得不再昂貴。
本本申請中，“高純”是指至少一種類胡蘿卜素的純度為至少95％，優(yōu)選地＞95％，優(yōu)選地＞96％，特別優(yōu)選地＞97％，非常特別優(yōu)選地＞98％，最優(yōu)選地＞99％。
可通過本發(fā)明方法產(chǎn)生的合適類胡蘿卜素均為天然的和人造的胡蘿卜素和葉黃素。至少一種類胡蘿卜素尤其選自蝦青素、玉米黃素、海膽酮、β-隱黃素、金盞花黃質(zhì)、金盞花玉紅質(zhì)、角黃素、3-羥海膽酮、3’-羥海膽酮、番茄紅素、β-胡蘿卜素、葉黃素、六氫番茄紅素、紅木素和八氫番茄紅素。優(yōu)選蝦青素或玉米黃素。類胡蘿卜素可以通過本發(fā)明方法單獨(dú)地或作為兩種或多種上述類胡蘿卜素的混合物獲得。類胡蘿卜素可以特別地如下文指出使用經(jīng)遺傳修飾生物(GMO)特定地產(chǎn)生。
食品被認(rèn)為是用來提供營養(yǎng)的組合物。這也包括補(bǔ)充營養(yǎng)的組合物。特別地，動物飼料和動物飼料添加劑被認(rèn)為是食品。
培養(yǎng)后，根據(jù)制備方案a)將生物量從培養(yǎng)肉湯中去除。為此目的，可使用技術(shù)人員熟悉的和經(jīng)常使用的固/液分離的任一方法。這特別包括機(jī)械方法，如基于利用重力、離心力、壓力、真空的過濾和離心?？墒褂玫姆椒ê驮O(shè)備另外尤其包括橫流過濾或膜技術(shù)如滲透、反向滲透、微過濾、超濾、納米過濾、餅狀過濾方法(如靠自動壓力過濾器(膜、架或腔)過濾壓力、(攪拌)壓力過濾、吸濾器、(真空)帶狀過濾器、(真空)鼓狀過濾器、旋轉(zhuǎn)過濾器、管狀過濾器)、通過連續(xù)地或分批地操作離心機(jī)或過濾離心機(jī)的離心方法(如顛倒過濾離心機(jī)、鏟式離心機(jī)、推式離心機(jī)、蠕動式/篩式離心機(jī)、滑動離心機(jī)、離析器或沉降式離心機(jī))，利用重力的方法，如浮選、沉降、沉浮分離和分選。生物量優(yōu)選通過沉降式離心機(jī)離心或通過膜過濾單元過濾從培養(yǎng)肉湯中去除。
根據(jù)制備方案b)的第二步在培養(yǎng)肉湯中產(chǎn)生勻漿的固體懸浮液。為此目的，可使用技術(shù)人員熟悉和常用的任一方法。文中(在實(shí)驗室規(guī)模上)特別使用分散劑如UltraTurrax_?？梢詫?xì)胞進(jìn)行破裂但這并不是必需的。
如果必要，培養(yǎng)肉湯可以經(jīng)脫水而獲得適合的＞2％并＜50％的固體含量。為此目的，可使用技術(shù)人員熟悉并常用的任一固/液體分離方法。這特別包括機(jī)械方法，如基于利用重力、離心力、壓力、真空的過濾和離心?？墒褂玫姆椒ê驮O(shè)備另外尤其包括橫流過濾或膜技術(shù)如滲透、反向滲透、微過濾、超濾、納米過濾、餅狀過濾方法(如靠自動壓力過濾器(膜、架或腔)過濾壓力、(攪拌)壓力過濾、吸濾器、(真空)帶狀過濾器、(真空)鼓狀過濾器、旋轉(zhuǎn)過濾器、管狀過濾器)、通過連續(xù)地或分批地操作離心機(jī)或過濾離心機(jī)的離心方法(如顛倒過濾離心機(jī)、鏟式離心機(jī)、推式離心機(jī)、蠕動式/篩式離心機(jī)、滑動離心機(jī)、離析器或沉降式離心機(jī))，利用重力的方法，如浮選、沉降、沉浮分離和分選。生物量優(yōu)選通過沉降式離心機(jī)離心或通過膜過濾單元過濾從培養(yǎng)肉湯中去除。培養(yǎng)肉湯隨后得到干燥。再一次，可使用技術(shù)人員所知的任何方法和設(shè)備。特別適合的設(shè)備為用于熱干燥的設(shè)備如用于對流、接觸和輻射干燥的設(shè)備，例如碟、腔、溝、平網(wǎng)、盤、旋轉(zhuǎn)鼓、自由降落軸、篩帶、流注、流化床、扁平烤盤、細(xì)膜、罐、帶、篩鼓、螺旋槳、翻滾、接觸盤、紅外線、微波和冷凍干燥器、噴霧干燥器或整合有流化床的噴霧干燥器，其根據(jù)需要通過蒸汽、油、氣或電流得到加熱，并根據(jù)需要在減壓條件下操作。根據(jù)設(shè)備，操作模式可以是連續(xù)的或分批的。另外或與此結(jié)合，可以使用上文已經(jīng)描述的固/液體分離的機(jī)械方法。
然而，如WO 97/36996 A2所公開的通過擠壓法使成顆粒狀是不必要的。干燥過程使得食品穩(wěn)定并易儲藏。
培養(yǎng)肉湯特別是經(jīng)噴霧干燥。如DE 101 04 494 A1，DE-A-12 11 911或EP 0 410 236 A1中所公開，優(yōu)選噴霧干燥過程。另外，參照R_mpp LexikonChemie CD-ROM Version 2.0，Georg Thieme Verlag，1999，“Sprühtrocknung”und R_mpp Lexikon Biotechnologie，Georg ThiemeVerlag，1992，“Zerst_ubungstrocknung”一文，噴霧干燥有產(chǎn)品在干燥機(jī)高溫區(qū)停留時間短的優(yōu)點(diǎn)，因此完成特別溫和的干燥過程。
噴霧干燥時選擇入口溫度約115℃-180℃，優(yōu)選120℃-130℃，且排出溫度約50℃-80℃，優(yōu)選55℃-70℃。干燥過程優(yōu)選使用的氣體是氮?dú)狻?br> 根據(jù)需要，可加入助流劑如硅酸等以得到更好的流動性。使用惰性載體材料，也就是低分子量的無機(jī)載體如NaCl、CaCO3、Na2SO4或MgSO4，有機(jī)載體如葡萄糖、果糖、蔗糖、糊精或淀粉制品(黑麥、大麥、燕麥粉、麥麩)是方便的。
干燥的產(chǎn)品基于干重優(yōu)選地具有少于10％的殘留水分，優(yōu)選地少于5％。其類胡蘿卜素含量基于干重在0.05到20％之間，特別是1到10％之間。
這種方法產(chǎn)生的食品可以直接使用或如DE 101 04 494 A1中所公開的那樣通過進(jìn)一步加料而加工。
根據(jù)可選方案IIC，在培養(yǎng)之后和干燥之前，生物量首先從培養(yǎng)肉湯中去除。為此目的，可使用技術(shù)人員熟悉和常用的任一固/液體分離方法，如可使用上文用于脫水已經(jīng)提到的方法。生物量優(yōu)選地通過沉降式離心機(jī)離心或膜過濾從培養(yǎng)肉湯中分離。
隨后，生物量任選地用類胡蘿卜素不可溶的溶劑特別是水洗滌，因此特別是水溶性成分得到去除。根據(jù)需要該步驟可以用進(jìn)一步的類胡蘿卜素不溶的溶劑(如醇)作為補(bǔ)充，雖然這在發(fā)明范疇內(nèi)是不必需的并且為了避免浪費(fèi)不是優(yōu)選的。
隨后，要進(jìn)行滅菌和隨后的或伴隨的破壞生物量中的細(xì)胞。滅菌殺死微生物并終止了可能存在的酶活性。這對于生物量或其中存在的物質(zhì)特別是類胡蘿卜素的穩(wěn)定性和避免降解是重要的。
滅菌可以使用技術(shù)人員熟悉的常規(guī)方法進(jìn)行。這包括用蒸汽，特別是在高于120℃在壓力(≥1巴)并≥約20分鐘條件下滅菌，也包括用如紫外線、微波、γ射線或β射線的高能量輻射處理。在本發(fā)明范圍內(nèi)的滅菌為優(yōu)選地使用蒸汽或微波輻射滅菌。
隨后的或伴隨細(xì)胞的破裂使在細(xì)胞中存在的類胡蘿卜素釋放出來。細(xì)胞的破裂同樣可以使用技術(shù)人員知曉的任何常規(guī)方法進(jìn)行。這包括機(jī)械的和非機(jī)械的方法。機(jī)械的方法包括干磨、濕磨、攪拌、勻漿(如在高壓勻漿器中)和使用超聲或微波。適合的非機(jī)械方法是物理的、化學(xué)的和生物化學(xué)的方法。這些包括短時間加熱、短時間冷凍、滲壓休克、干燥、用酸或堿和酶破壞處理。但是有利地，使用用于滅菌的方法來破裂細(xì)胞。因此，同樣優(yōu)選用蒸汽或微波輻射進(jìn)行細(xì)胞破裂。
滅菌和/或細(xì)胞破裂可以連續(xù)地或分批地進(jìn)行。
滅菌和/或細(xì)胞破裂可以在用于培養(yǎng)的生物反應(yīng)器中或在其他設(shè)備如高壓滅菌鍋中進(jìn)行。如果方法是連續(xù)的，可以使用微波-使用WO 01/83437 A1中所公開的方法和相應(yīng)的設(shè)備。
在提取之前，根據(jù)需要，生物量經(jīng)過干燥和/或勻漿處理。再一次，這里可使用技術(shù)人員知曉的任何常規(guī)方法和設(shè)備。特別適合的設(shè)備為用于熱干燥的設(shè)備如用于對流、接觸和輻射干燥的設(shè)備，例如碟、腔、溝、平網(wǎng)、盤、旋轉(zhuǎn)鼓、自由降落軸、篩帶、流注、流化床、扁平烤盤、細(xì)膜、罐、帶、篩鼓、螺旋槳、翻滾、接觸盤、紅外線、微波和冷凍干燥器、噴霧干燥器或整合有流化床的噴霧干燥器，其根據(jù)需要通過蒸汽、油、氣或電流得到加熱，并根據(jù)需要在減壓條件下操作。根據(jù)設(shè)備，操作模式可以是連續(xù)的或分批的。另外或與此結(jié)合，可以使用上文已經(jīng)描述的固/液體分離的機(jī)械方法。
然而，如WO 97/36996 A2所公開的通過擠壓法使成顆粒狀是不必要的。
隨后，類胡蘿卜素通過類胡蘿卜素可溶的溶劑從破裂的生物量中部分提取并從生物量中分離所述溶劑。溶劑和生物量因此都包含類胡蘿卜素，大多數(shù)所述類胡蘿卜素優(yōu)選地存在于溶劑中。
然后從溶劑中分離高純度的類胡蘿卜素，而生物量經(jīng)進(jìn)一步加工以產(chǎn)生高品質(zhì)的含類胡蘿卜素的食品，所述食品由于前述的細(xì)胞破裂，也具有很好的類胡蘿卜素生物利用率。
因此，部分提取是指從生物量中故意的不完全提取類胡蘿卜素(見上文)。在本發(fā)明范疇內(nèi)通過所述提取得到的類胡蘿卜素優(yōu)選少于生物量中類胡蘿卜素的總量的100％。這有很大的優(yōu)勢，因為提取復(fù)雜性的提高與生物量中類胡蘿卜素含量的下降不成正比。
用于提取的溶劑是能溶解類胡蘿卜素的溶劑，如己烷、乙酸乙脂、二氯甲烷或超臨界二氧化碳。根據(jù)本發(fā)明優(yōu)選使用的溶劑是二氯甲烷或超臨界二氧化碳，當(dāng)用超臨界二氧化碳時，可直接通過膨脹二氧化碳將其中存在的類胡蘿卜素隨后轉(zhuǎn)移至二氯甲烷或得到目的產(chǎn)物。就此而論，選擇溶劑的量和混合的次數(shù)，這樣就從生物量中提取到所需要的類胡蘿卜素量。更具體地，提取步驟只進(jìn)行一次，這在技術(shù)上和經(jīng)濟(jì)上都是明智的(見上文)。
提取可以用任何常規(guī)的方法和設(shè)備進(jìn)行。更具體地，如果生物量得到破裂但沒有干燥，要進(jìn)行液/液體提取(類胡蘿卜素以可溶的形式存在于液體細(xì)胞組分中并從其中得到提取)，如果生物量已經(jīng)得到干燥，要進(jìn)行固/液體提取。在特定的溫度范圍內(nèi)可使用冷和熱提取，包括如通過振蕩、用堿、通過煮沸、和通過消化提取的連續(xù)(如索個利特提取、過濾和滲濾)和不連續(xù)過程。它們也可以通過逆流方法進(jìn)行。
使用如發(fā)泡柱、搏動柱、帶有旋轉(zhuǎn)內(nèi)部裝置的柱子、混合澄清槽或攪動罐的液/液體提取是可能的。
固/液體提取可以通過常規(guī)設(shè)備得到實(shí)現(xiàn)。優(yōu)選使用攪動罐或混合澄清裝置。
備選地，破壞細(xì)胞可以不先去除發(fā)酵培養(yǎng)基，隨后直接從生物量中如通過沉降分離產(chǎn)生的類胡蘿卜素懸液。類胡蘿卜素懸液隨后置于二氯甲烷中并進(jìn)一步加工，或備選地，通過用不同水溶液洗滌得到純化。
高純度的類胡蘿卜素是從所使用的溶劑中通過結(jié)晶所述類胡蘿卜素并分離類胡蘿卜素晶體，特別是通過過濾分離。剩余的母液可以經(jīng)過蒸餾后重新進(jìn)入該過程，這樣減少了產(chǎn)品損失且不費(fèi)勁。
結(jié)晶可以按常規(guī)進(jìn)行。另外，可參照R_mpp Lexikon Chemie CD-ROMVersion 2.0，Georg Thieme Verlag，1999，“Kristallisation”一書。
結(jié)晶優(yōu)選通過用類胡蘿卜素不溶的溶劑逐漸取代原溶液而實(shí)施。因此，類胡蘿卜素的溶解性不斷下降直到所述類胡蘿卜素以純晶體的形式沉降下來。此處優(yōu)選使用“低級醇”或水。低級醇是指具有1到4個碳原子的脂族醇。這包括甲醇、乙醇、丙醇、異丙醇、正丁醇、叔丁醇和仲丁醇。優(yōu)選使用甲醇。
就此而論，可以加熱類胡蘿卜素溶液，溫度優(yōu)選地保持＜100℃，特別＜60℃，以使二氯甲烷蒸餾掉。使用降低的壓力也是很方便的。類胡蘿卜素晶體于是得到分離并且這可以通過常規(guī)方法特別是通過過濾實(shí)施。如果需要，可以繼之以進(jìn)一步任選的干燥和/或純化步驟。但是這些步驟不是必需的，因為類胡蘿卜素晶體已經(jīng)是高純度的。
類胡蘿卜素作為高純晶體而獲得并具有至少95％，優(yōu)選地＞95％，優(yōu)選地＞96％，特別優(yōu)選地＞97％，非常特別優(yōu)選地＞98％，最優(yōu)選地＞99％的純度。
可獲得的產(chǎn)量基于在培養(yǎng)肉湯中存在的量在45％和95％之間，優(yōu)選地在70％和95％之間(0.5-15g/L，優(yōu)選地1-10g/L)。
為了進(jìn)一步加工同樣含有類胡蘿卜素的生物量以得到高品質(zhì)的食品，首先要從含有類胡蘿卜素的生物量中去除剩余溶劑。這優(yōu)選通過蒸餾或用蒸汽“除去(stripping)”的方法得到實(shí)現(xiàn)(參見R_mpp Lexikon ChemieCD-ROM Version 2.0，Georg Thieme Verlag，1999，″Strippen″)。
根據(jù)需要，這可以繼之以在經(jīng)過去除的上述培養(yǎng)肉湯中均勻懸浮生物量，其固體含量經(jīng)觀測應(yīng)為＞100g/L并且＜600g/L以使隨后干燥或懸浮生物量生產(chǎn)食品沒有技術(shù)困難地得到實(shí)現(xiàn)，也就是說懸浮液必須是可吸取的。適合的干燥方法是已經(jīng)提到的全部方法和設(shè)備。更具體地，干燥過程可用噴霧干燥，如DE 101 04 494 A1所公開地進(jìn)行。
噴霧干燥時選擇入口溫度約100℃-180℃，優(yōu)選120℃-130℃，且排出溫度約50℃-80℃，優(yōu)選55℃-70℃。干燥過程優(yōu)選使用的氣體是氮?dú)狻?br> 以這種方法產(chǎn)生的食品可以直接使用或如DE 101 04 494 A1中所公開通過進(jìn)一步加料而加工。
食品被認(rèn)為是用來提供營養(yǎng)的組合物。這也包括補(bǔ)充營養(yǎng)的組合物。特別地，動物飼料和動物飼料添加劑被認(rèn)為是食品。另外，可參照R_mppLexikon Chemie CD-ROM Version 2.0，Georg Thieme Verlag，1999，“Nahrungsmittel”。
干燥的產(chǎn)品基于干重優(yōu)選地具有少于5％的殘留水分。其類胡蘿卜素含量基于干重在0.05到20％之間，特別是1到10％之間。所需要的類胡蘿卜素含量可通過提取的程度而控制(見上文)。
因此，通過根據(jù)本發(fā)明的方法可以獲得的食品包括不需要添加的已經(jīng)產(chǎn)生的大量類胡蘿卜素。而且，由于所述食品除了含有至少一種類胡蘿卜素外也含有生物量，食品的營養(yǎng)物含量提高了。根據(jù)優(yōu)選的備選方案，營養(yǎng)物含量的提高特別大，因為它除了至少一種類胡蘿卜素和生物量，另外包括發(fā)酵的全部培養(yǎng)基組分。結(jié)果，除了水介質(zhì)外事實(shí)上沒有廢品，而該水介質(zhì)可以在純化車間很容易得到純化。另外，全部產(chǎn)生的類胡蘿卜素量沒有或僅有少量的損失，因為為了提取全部量的類胡蘿卜素不需要進(jìn)行造成大量損失的分離或加工步驟。
上述本發(fā)明方法使用的所有溶劑都是盡可能純化的并隨后再使用或再進(jìn)入該過程。更具體地，使用的二氯甲烷在溶劑替換的時候已經(jīng)得到純化并其后可以再次使用。低級醇或甲醇例如可以通過蒸餾得到純化并同樣再使用。唯一的廢品是蒸餾底，它可以與水介質(zhì)一起在純化車間無風(fēng)險地直接得到控制而最終實(shí)際的廢品僅是少量的淤泥。因此，所述的方法基本上是無浪費(fèi)的。
以下本發(fā)明在實(shí)施例基礎(chǔ)上更詳細(xì)地進(jìn)行闡明。
A)培養(yǎng)三孢布拉霉以下培養(yǎng)基用于發(fā)酵三孢布拉霉以產(chǎn)生類胡蘿卜素培養(yǎng)基1葡萄糖 10.00g/l棉籽油 30.00g/l大豆油 30.00g/l糊精 60.00g/l棉籽粉 75.00g/lTrition X 100 1.20g/l維生素C6.00g/l乳酸 2.00g/l磷酸二氫鉀 0.50g/l水合硫酸錳 100mg/l鹽酸硫胺素 2mg/l異煙肼 0.75g/l將pH調(diào)至6.5。
培養(yǎng)基2葡萄糖 20g/l天冬酰胺 2.00g/l磷酸二氫鉀 5.00g/l七水合硫酸鎂 0.50g/l
氯化鈣 28mg/l鹽酸硫胺素 1.00mg/l檸檬酸 2.00mg/l九水合硝酸鐵 1.50mg/l七水合硫酸鋅 1.00mg/l水合硫酸錳 0.30mg/l五水合硫酸銅 0.05mg/l二水合鉬酸鈉 0.05mg/l培養(yǎng)基3葡萄糖 70.00g/l天冬酰胺 2.00g/l酵母提取物 1.00g/l磷酸二氫鉀 1.50g/l七水合硫酸鎂 0.50g/lSpan 201.00g/l鹽酸硫胺素 5.0mg/l將pH值調(diào)至5.5。
在每種情況下將分別地包含108個(對于培養(yǎng)基2)，107個(對于培養(yǎng)基1和3)的三孢布拉霉ATCC14272交配型(-)的孢子懸液接種于200ml上述培養(yǎng)基。在每種情況下在1升帶擋板的三角瓶中培養(yǎng)。對每一種培養(yǎng)基，準(zhǔn)備6個相同的燒瓶，在搖床上28℃，140轉(zhuǎn)/分鐘培養(yǎng)7天。
B)三孢布拉霉的遺傳修飾材料和方法除了另外說明分子遺傳學(xué)操作是根據(jù)Current Protocols in MolecularBiology(Ausubel等，1999，John Wiley & Sons)進(jìn)行的。
菌株和生長條件三孢布拉霉ATCC14271(交配型(+))和ATCC14272(-)(野生型、交配型(-))從美國典型培養(yǎng)物保藏中心獲得。將三孢布拉霉于MEP培養(yǎng)基(麥芽汁-蛋白胨培養(yǎng)基)生長30g/l麥芽汁(Difco)、3g/l蛋白胨(Soytone，Difco)、20g/l瓊脂，調(diào)pH至5.5，加水至1000ml，28℃培養(yǎng)。
根據(jù)Hoekema等(1983，Nature 303179-180)，將根癌農(nóng)桿菌LBA4404于28℃農(nóng)桿菌最小培養(yǎng)基(AMM)中培養(yǎng)24小時10mM K2HPO4，10mMKH2PO4，10mM葡萄糖，MM鹽(2.5mM NaCl，2mM MgSO4，700μMCaCl2，9μM FeSO4，4mM(NH4)2SO4)。
轉(zhuǎn)化根癌農(nóng)桿菌將pBinAHyg質(zhì)粒電穿孔進(jìn)入農(nóng)桿菌菌株LBA4404(Hoekema等，1983，Nature 303179-180)(Mozo和Hooykaas，1991，Plant Mol.Biol.16917-918)。在農(nóng)桿菌生長時用以下抗生素進(jìn)行篩選50mg/l利福平(篩選根癌農(nóng)桿菌染色體)、30mg/l鏈霉素(篩選輔助質(zhì)粒)、100mg/l卡那霉素(篩選雙元載體)。
轉(zhuǎn)化三孢布拉霉在AMM中培養(yǎng)24小時后，將農(nóng)桿菌用誘導(dǎo)培養(yǎng)基(IMMM鹽，40mM MES(pH5.6)，5mM葡萄糖，2mM磷酸鹽，0.5％甘油，200μM乙酰丁香酮)稀釋至OD600為0.15并于IM中再次過夜生長至OD600約為0.6用于轉(zhuǎn)化。
共培養(yǎng)布拉霉菌ATCC14271或ATCC14272與農(nóng)桿菌時，100μl農(nóng)桿菌懸液與100μl布拉霉菌孢子懸液(于0.9％NaCl中的107孢子/ml)混合并用無菌的方法將其分布在IM瓊脂糖平板(IM+18g/l瓊脂糖)上的尼龍膜(Hybond N，Amersham)。26℃培養(yǎng)3天后，將膜轉(zhuǎn)于MEP-瓊脂平板(30g/l麥芽汁、3g/l蛋白胨、pH5.5，18g/l瓊脂)。為了篩選轉(zhuǎn)化的布拉霉細(xì)胞，培養(yǎng)基含有濃度為100mg/l的潮霉素并為了針對農(nóng)桿菌進(jìn)行選擇，含有100mg/l的頭孢噻肟。在26℃培養(yǎng)約7天。繼之以將菌絲體轉(zhuǎn)入新鮮的選擇平板。所得的孢子用0.9％NaCl漂洗并鋪于CM17-1瓊脂(3g/l葡萄糖，200mg/l L-天冬酰胺，50mg/l MgSO4×7H2O，150mg/lKH2PO4，25μg/l鹽酸硫胺素，100mg/l酵母提取物，100mg/l脫氧膽酸鈉，100mg/L潮霉素，100mg/L頭孢噻肟，pH5.5，18g/l瓊脂)。單個的經(jīng)遺傳修飾孢子是通過將其單獨(dú)地置于選擇培養(yǎng)基上用來自BectonDickson(Modell Vantage+Diva Option)的FACS設(shè)備分離。
用MNNG突變?yōu)榱藴p少每個孢子細(xì)胞核的數(shù)目，孢子懸液用MNNG(N-甲基-N’-硝基亞硝基胍)處理。為此，首先制備含有1×107孢子/ml于Tris/HCl緩沖液，pH7.0的孢子懸液。孢子懸液與終濃度為100μg/ml的MNNG混合。在MNNG中溫浴時間要選擇在孢子存活率為約5％。與MNNG孵育以后，用1g/l溶于50mM磷酸緩沖液的Span 20洗滌三次并涂布孢子。
篩選同核細(xì)胞同核的三孢布拉霉carB-細(xì)胞通過與布拉克須霉相似的實(shí)驗方法(Roncero等，1984，Mutation Research，125195-204)，修改為在5-碳-5-脫氮核黃素(1μg/ml)和潮霉素100(μg/ml)存在下培養(yǎng)而篩選。
通過農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化制備遺傳修飾的三孢布拉霉■制備重組pBinAHyg質(zhì)粒gpdA-hph-trpC-盒是從pANsCos1質(zhì)粒(

圖1，Osiewacz，1994，Curr.Genet.2687-90，SEQ ID NO4)中作為BglII/HindIII片段分離并連接入由BamHI/HindIII切開的雙元載體pBin19中(Bevan，1984，Nucleic Acids Res.128711-8721)。以此方式獲得的載體稱作pBinAHyg(圖2，SEQ ID NO3)并包含在gpd啟動子(SEQ ID NO1)控制下的大腸桿菌潮霉素抗性基因(hph)和來自構(gòu)巢曲霉的trpC終止子(SEQ ID NO2)以及相應(yīng)的農(nóng)桿菌DNA轉(zhuǎn)移所需的邊界序列。在下文示例性實(shí)施方案中提及的載體為pBinAHyg衍生物。
將pBinAHyg和pBinAHyg衍生物轉(zhuǎn)入根癌農(nóng)桿菌將pBinAHyg質(zhì)粒轉(zhuǎn)入農(nóng)桿菌是通過以下實(shí)施例得到描述的。其衍生物用同樣的方法轉(zhuǎn)入農(nóng)桿菌。
pBinAHyg質(zhì)粒經(jīng)電穿孔進(jìn)入農(nóng)桿菌菌株LBA4404中(Hoekema等，1983，Nature 303179-180)(Mozo和Hooykaas，1991，Plant Mol.Biol.16917-918)。農(nóng)桿菌生長過程中用以下抗生素進(jìn)行篩選利福平50mg/l(篩選根癌農(nóng)桿菌染色體)、鏈霉素30mg/l(篩選輔助質(zhì)粒)、卡那霉素100mg/l(篩選雙元載體)。
將pBinAHyg和pBinAHyg衍生物轉(zhuǎn)入三孢布拉霉農(nóng)桿菌在AMM中培養(yǎng)24小時后，將農(nóng)桿菌用誘導(dǎo)培養(yǎng)基(IMMM鹽，40mM MES(pH5.6)，5mM葡萄糖，2mM磷酸鹽，0.5％甘油，200μM乙酰丁香酮)稀釋至OD660為0.15并于IM中再次過夜生長至OD660約為0.6用于轉(zhuǎn)化。
共培養(yǎng)三孢布拉霉(B.t.)與根癌農(nóng)桿菌(A.t.)時，100μl農(nóng)桿菌懸液與100μl布拉霉菌孢子懸液(107孢子/ml于0.9％NaCl)混合并用無菌的方法將其分布在IM瓊脂糖平板(IM+18g/l瓊脂糖)上的尼龍膜(HybondN，Amersham)。26℃培養(yǎng)3天后，將膜轉(zhuǎn)移于MEP-瓊脂平板(30g/l麥芽汁、3g/l蛋白胨、pH 5.5，18g/l瓊脂)。
為了篩選轉(zhuǎn)化的布拉霉細(xì)胞，培養(yǎng)基含有濃度為100mg/l的潮霉素并為了針對農(nóng)桿菌進(jìn)行選擇，含有100mg/l的頭孢噻肟。在26℃培養(yǎng)約7天。繼之以將菌絲體轉(zhuǎn)入新鮮的選擇平板。所得的孢子用0.9％NaCl漂洗并鋪于CM17-1瓊脂3g/l葡萄糖，200mg/l L-天冬酰胺，50mg/l MgSO4×7H2O，150mg/l KH2PO4，25μg/l鹽酸硫胺素，100mg/l酵母提取物，100mg/l脫氧膽酸鈉，100mg/L潮霉素，100mg/L頭孢噻肟，pH 5.5，18g/l瓊脂)。重復(fù)三遍將孢子轉(zhuǎn)移入新鮮的選擇平板。這樣，就分離到三孢布拉霉轉(zhuǎn)化子GMO3005。備選地，通過將孢子分別地應(yīng)用于含有100mg/l頭孢噻肟、100mg/l潮霉素的CM-17瓊脂，用BectonDickinson FacsVantage+Diva選擇方法來篩選GMO(經(jīng)遺傳修飾的生物)。在這種情況下，形成的真菌菌絲體僅為孢子已經(jīng)經(jīng)過遺傳修飾的真菌菌絲體。
在三孢布拉霉中檢測由于轉(zhuǎn)入pBinAHyg和pBinAHyg衍生物的遺傳修飾在三孢布拉霉中檢測轉(zhuǎn)移是通過以下用于pBinAHyg的實(shí)施例得到描述的。衍生物轉(zhuǎn)移的檢測是通過類似的方法實(shí)施的。
將105-107三孢布拉霉GMO3005轉(zhuǎn)化子孢子接種于200ml MEP培養(yǎng)基(30g/l麥芽汁、3g/l蛋白胨、pH5.5)中并在旋轉(zhuǎn)式搖床上200轉(zhuǎn)/分鐘、26℃孵育7天。為了檢測成功的轉(zhuǎn)化子，從菌絲體中分離DNA(Peqlab真菌DNA小量試劑盒)并使用PCR(程序94℃1分鐘，然后94℃1分鐘、58℃1分鐘、72℃1分鐘，進(jìn)行30個循環(huán))。
用Hph正向引物(5’-CGATGTAGGAGGGCGTGGATA，SEQ.IDNO.5)和hph反向引物(5’-GCTTCTGCGGGCGATTTGTGT，SEQ.IDNO.6)檢測潮霉素抗性基因(hph)。預(yù)期的hph片段長度是800bp。
使用nptIII正向引物(5’-TGAGAATATCACCGGAATTG，SEQ.IDNO.7)和nptIII反向引物(5’-AGCTCGACATACTGTTCTTCC，SEQ.IDNO.8)擴(kuò)增卡那霉素抗性基因ntpIII并因此作為農(nóng)桿菌對照。預(yù)期的nptIII片段長度為700bp。
使用引物MAT292(5’-GTGAATGGAAATCCCATCGCTGTC，SEQ.ID NO.9)和MAT293(5’-AGTGGGTACTCTAAAGGCCATACC，SEQ.ID NO.10)擴(kuò)增甘油醛3-磷酸脫氫酶基因gpd1片段并因此作為三孢布拉霉對照。預(yù)期的gpd1片段長度為500bp。
圖3描述了三孢布拉霉DNA在標(biāo)準(zhǔn)凝膠中的PCR結(jié)果。凝膠泳道按以下順序上樣1)100bp大小分子量標(biāo)記物(100bp-1kb)2)B.t.GMO 3005 nptIII正向/nptIII反向引物3)B.t.GMO 3005 hph正向/hph反向引物4)B.t.GMO 3005 MAT292/MAT293(gpd)引物5)具pBinAHyg nptIII正向/nptIII反向引物質(zhì)粒的A.t.
6)具pBinAHyg hph正向/hph反向引物質(zhì)粒的A.t.
7)B.t.14272野生型 nptIII正向/nptIII反向引物
8)B.t.14272野生型 hph正向/hph反向引物9)B.t.14272野生型 MAT292/MAT293(gpd)引物在三孢布拉霉DNA中檢測潮霉素抗性基因(hph)和作為陽性對照的甘油醛3-磷酸脫氫酶基因(gpd1)。作為對照，沒有檢測nptIII。
因此，檢測了通過農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化形成的三孢布拉霉遺傳修飾。
分離同型核三孢布拉霉遺傳修飾生物制備同核菌株成功地將pBinAHyg載體和pBinAHyg衍生物轉(zhuǎn)入三孢布拉霉中產(chǎn)生了遺傳修飾生物。然而，在三孢布拉霉遺傳修飾生物中，布拉霉菌在全部無性和有性細(xì)胞周期中都是多核的。所以，外源DNA載體通常僅插入一個細(xì)胞核中。然而，目的是獲得外源DNA載體已插入所有細(xì)胞核中的三孢布拉霉菌株，即目的是同核重組的真菌菌絲體。
為了制備這種同核細(xì)胞，重組菌株的孢子懸液先經(jīng)MNNG處理。為此，先制備在pH7.0的Tris/鹽酸緩沖液中含有1×107孢子/ml的孢子懸液。孢子懸液與MNNG混合至MNNG終濃度為100μg/ml。在MNNG中溫浴的時間要通過孢子的存活率約為5％的方式得到選擇。與MNNG溫浴以后，用在pH7.0 50mM磷酸緩沖液中的1g/l Span 20洗滌三次并涂平板。
1)通過FACS(熒光活化的細(xì)胞分選)的方法制備同核重組菌株一小部分的三孢布拉霉孢子或遺傳修飾的三孢布拉霉菌株天然是同核的。為了產(chǎn)生含有pBinAHyg或pBinAHyg衍生物的外源DNA的同核重組菌株，同核的孢子經(jīng)FACS方法分選并涂于含有100mg/l頭孢噻肟和100mg/l潮霉素的MEP平板(30g/l麥芽汁，3g/l蛋白胨，pH5.5，18g/l瓊脂)。產(chǎn)生的菌絲體是同核的。為了進(jìn)行FACS，用10ml 50mMol Tris-鹽酸+0.1％Span20洗掉三天大的孢子。孢子濃度為每毫升0.5到0.8×107孢子。將1ml DMSO和10μl Syto 11(溶于DMSO的染料貯存液，MolecularProbes No.S-7573)加入至9ml的孢子懸液。繼之以于30℃染色2小時。通過BectonDickinson FacsVantage+Diva選擇進(jìn)行篩選和應(yīng)用。首先，為了從聚集體和污染物中分離單個孢子，進(jìn)行了大小篩選。這些孢子隨后用于根據(jù)其熒光分選(激發(fā)光＝488nm，發(fā)射光＝530nm)。熒光頻率分布高斯曲線的左肩包含單核孢子。
然后將孢子鋪于MEP瓊脂板，并產(chǎn)生新孢子。
這些孢子通過與Roncero等相似的方法涂于含有5-碳-5-脫氮核黃素和加之以潮霉素的平板上。
這使得基因型為hygR和dar-的同核細(xì)胞得到篩選。
2)通過減少細(xì)胞核的數(shù)量并用FACS篩選制備同核菌株為了減少每個孢子細(xì)胞核的數(shù)量，孢子懸液在篩選前用MNNG(N-甲基-N’-硝基亞硝基胍)處理，這樣通過化學(xué)誘變劑達(dá)到了細(xì)胞核數(shù)量的減少。
為此，首選制備在pH7.0的Tris/鹽酸緩沖液中含有1×107孢子/ml的孢子懸液。孢子懸液與MNNG混合至MNNG終濃度為100μg/ml。在MNNG中溫浴的時間要通過孢子的存活率約為5％的方法選擇。與MNNG溫浴以后，用在pH7.0的50mM磷酸緩沖液中1g/l Span 20洗滌三次并通過1)中所述方法分選和篩選。
作為可供選擇的方法，如Cerdá-Olmedo和Patricia Reau在MutationRes.，9(1970)，369-384中所描述，可以通過用X-射線和紫外線減少孢子中細(xì)胞核的數(shù)量。
3)通過篩選隱性選擇標(biāo)記制備同核菌株用來篩選同核菌絲體的適合的隱性選擇標(biāo)記是例如隱性選擇標(biāo)記pyrG。三孢布拉霉野生型菌株是pyrG+。這些菌株不能在嘧啶類似物5-氟乳清酸(FOA)存在下生長，因為它們將FOA通過乳清酸核苷5’-單磷酸脫羧酶轉(zhuǎn)化為致命的代謝物。遺傳修飾的pyrG--同核布拉霉菌缺少乳清酸核苷5’-單磷酸脫羧酶活性。因此，這些pyrG-菌株不能利用5-氟乳清酸。所以，這些菌株在FOA和尿嘧啶存在下生長。如果pyrG-突變和外源DNA插入物在單核孢子的細(xì)胞核上偶聯(lián)，這些孢子可形成同核重組真菌菌絲體。
首先，通過將三孢布拉霉的pyrG片段(SEQ ID NO65)插入到pBinAHyg中產(chǎn)生了pBinAHygBTpyrG-SCO質(zhì)粒(SEQ.ID NO.36，圖4)。將所述質(zhì)粒轉(zhuǎn)化入三孢布拉霉中并由于同源重組導(dǎo)致pyrG的破壞。
具有pyrG-表型的同核三孢布拉霉GMO如下得到篩選。農(nóng)桿菌介導(dǎo)的pBinAHygBTpyrG-SCO轉(zhuǎn)化如上所述進(jìn)行，涂于含有100mg/l頭孢噻肟和100mg/l潮霉素的MEP培養(yǎng)基(30g/l麥芽汁、3g/l蛋白胨、pH5.5、18g/l瓊脂)上。每個瓊脂平板用10ml 50mMol Tris-鹽酸+0.1％ Span20洗掉轉(zhuǎn)化子孢子。孢子濃度為每毫升0.5到0.8×107孢子。孢子隨后被涂于含有100mg/l頭孢噻肟和100mg/l潮霉素的FOA培養(yǎng)基上。FOA培養(yǎng)基根據(jù)Sutter，1975，PNAS，72127每升含有20g葡萄糖、1g FOA、50mg尿嘧啶、200ml檸檬酸緩沖液(0.5M、pH4.5)和40ml微量鹽溶液。同核pyrG-突變體顯示在含有尿嘧啶的FOA培養(yǎng)基上生長但將其涂于不含尿嘧啶的FOA培養(yǎng)基上時卻不能生長。同樣的方法，下面描述了從三孢布拉霉GMO制備同核GMO以產(chǎn)生葉黃素。
作為可供選擇的方法，可以根據(jù)Roncero等的方法將孢子涂于含有5-碳-5-脫氮核黃素和加之以潮霉素的培養(yǎng)基上(Roncero等，1984，MutationResearch，125195-204)。這使得基因型為hygR和dar-的同核細(xì)胞得到篩選。
根據(jù)這一原理，產(chǎn)生了具有hygR和dar-表型的三孢布拉霉同核菌株。
制備用于產(chǎn)生類胡蘿卜素和類胡蘿卜素前體的三孢布拉霉遺傳修飾生物的示例性實(shí)施方案下面提到的質(zhì)粒是通過“重疊延伸PCR”法并通過隨后將擴(kuò)增產(chǎn)物插入pBinAHyg質(zhì)粒中產(chǎn)生的。重疊延伸PCR方法是如Innis等(編輯)PCRprotocolsa guide to methods and applications，Academic Press，San Diego一書所述進(jìn)行的。pBinAHyg衍生物的轉(zhuǎn)化和同核的遺傳修飾三孢布拉霉菌株的制備是如上所述進(jìn)行的。
用于產(chǎn)生玉米黃素的遺傳修飾的三孢布拉霉菌株以下質(zhì)粒(pBinAHyg衍生物)用于遺傳修飾三孢布拉霉以產(chǎn)生玉米黃素并因此尤其編碼羥化酶(crtZ)-ptef1-HPcrtZ，含有在三孢布拉霉ptef1啟動子控制下的來自雨生紅球藻Flotow NIES-144(登錄號AF162276)的HPcrtZ羥化酶基因(SEQ.IDNO.70)(序列pBinAHygBTpTEF1-HPcrtZ，SEQ.ID NO.37，圖5)；-p-carRA-HPcrtZ，含有在三孢布拉霉pcarRA啟動子控制下的來自雨生紅球藻Flotow NIES-144的HPcrtZ羥化酶基因(序列pBinAHygBTpcarRA-HPcrtZ，SEQ.ID NO.38，圖6)；-p-carB-HPcrtZ，含有在三孢布拉霉pcarB啟動子控制下的來自雨生紅球藻Flotow NIES-144的HPcrtZ羥化酶基因(序列pBinAHygBTpcarB-HPcrtZ，SEQ.ID NO.39，圖7)；-p-carRA-HPcrtZ-TAG-3’carA-IR，含有在三孢布拉霉pcarRA啟動子控制下的來自雨生紅球藻Flotow NIES-144的HPcrtZ羥化酶基因。反向重復(fù)結(jié)構(gòu)位于羥化酶基因的下游，該結(jié)構(gòu)源自于carA的3’末端和carA的下游區(qū)域(IR，SEQ.ID NO.74，“反向重復(fù)1”carA的約350bp，然后約200bp的“環(huán)”和然后約350bp的“反向重復(fù)2”)(序列pBinAHyg-BTpcarRA-HPcrtZ-TAG-3’carA-IR，SEQ.ID NO.40，圖8)；-p-carRA-HPcrtZ-GCG-3’carA-IR，含有在三孢布拉霉pcarRA啟動子控制下的來自雨生紅球藻Flotow NIES-144的HPcrtZ羥化酶基因。羥化酶基因與反向重復(fù)結(jié)構(gòu)融合，其中所述反向重復(fù)結(jié)構(gòu)源自于carA的3’末端和carA的下游區(qū)域(IR，SEQ.ID NO.74，“反向重復(fù)1”carA的約350bp，然后約200bp的“環(huán)”和然后約350bp的“反向重復(fù)2”)。因此，衍生融合蛋白由雨生紅球藻羥化酶和三孢布拉霉CarA的羰基端組成(序列pBinAHyg-BTpcarRA-HPcrtZ-GCG-3’carA-IR，SEQ.ID NO.41，圖9)。
-p-tef1-EUcrtZ，含有在ptef1啟動子控制下的來自嗜夏孢歐文氏菌20D3(登錄號D90087)的EUcrtZ羥化酶基因(SEQ.ID NO.71)(序列pBinAHygBTpTEF1-EUcrtZ，SEQ.ID NO.42，圖10)。
-p-carRA-EUcrtZ，含有在pcarRA啟動子控制下的來自嗜夏孢歐文氏菌20D3的EUcrtZ羥化酶基因(序列pBinAHygBTpcarRA-EUcrtZ，SEQ.ID NO.43，圖11)；-p-carB-EUcrtZ，含有在三孢布拉霉pcarB啟動子控制下的來自嗜夏孢歐文氏菌20D3的EUcrtZ羥化酶基因(序列pBinAHygBTpcarB-EUcrtZ，SEQ.ID NO.44，圖12)；-p-gpdA-HPcrtZ-t-crtZ，含有g(shù)pdA啟動子控制下的來自雨生紅球藻Flotow NIES-144的HPcrtZ羥化酶基因和t-crtZ終止子；即來自雨生紅球藻Flotow NIES-144的crtZ下游序列部分(SEQ.ID NO.73)(序列pBinAHyg-gpdA-HPcrtZ-tcrtZ，SEQ.ID NO.45，圖13)；-p-gpdA-BTcarR-HPcrtZ-BTcarA，含有來自三孢布拉霉的番茄紅素環(huán)化酶carR基因、來自雨生紅球藻Flotow NIES-144的HPcrtZ羥化酶基因和來自三孢布拉霉的八氫番茄紅素合成酶carA的基因融合并在構(gòu)巢曲霉gpdA啟動子控制下(序列pBinAHyg-carR_crtZ_carA，SEQ.ID NO.46，圖14)。
制備產(chǎn)生角黃素的遺傳修飾的三孢布拉霉菌株以下質(zhì)粒(pBinAHyg衍生物)用于遺傳修飾三孢布拉霉以產(chǎn)生角黃素并因此尤其編碼酮酶(crtW)-p-tef1-NPcrtW，含有來自點(diǎn)形念珠藻PCC73102(ORF148，登錄號NZ_AABC01000196)的NPcrtW酮酶基因(SEQ.ID NO.72)并在三孢布拉霉ptef1啟動子控制下(序列pBinAHygBTpTEF1-NpucrtW，SEQ.IDNO.47，圖15)；-p-carRA-NPcrtW，含有來自點(diǎn)形念珠藻PCC73102的NPcrtW酮酶基因并在三孢布拉霉pcarRA啟動子控制下(序列pBinAHygBTpcarRA-NpucrtW，SEQ.ID NO.48，圖16)；-p-carB-NPcrtW，含有來自點(diǎn)形念珠藻PCC73102的NPcrtW酮酶基因并在三孢布拉霉pcarB啟動子控制下(序列pBinAHygBTpcarB-NpucrtW，SEQ.ID NO.49，圖17)；制備產(chǎn)生蝦青素的遺傳修飾的三孢布拉霉菌株以下質(zhì)粒(pBinAHyg衍生物)用于遺傳修飾三孢布拉霉以產(chǎn)生蝦青素，即尤其編碼羥化酶(crtZ)和酮酶(crtW)-p-carRA-HPcrtZ-pcarRA-NPcrtW，含有來自雨生紅球藻FlotowNIES-144的HPcrtZ羥化酶基因和來自點(diǎn)形念珠藻PCC73102(ORF148，登錄號NZ-AABC01000196)的NPcrtW酮酶基因，二者在每種情況下都是在三孢布拉霉pearRA啟動子控制下(序列pBinAHygBTpcarRA-HPcrtZ-BTpcarRA-NpucrtW，SEQ.ID NO.50，圖18)；-p-carRA-EUcrtZ-pcarRA-NPcrtW，含有來自嗜夏孢歐文氏菌20D3(登錄號D90087)的EUcrtZ羥化酶基因和來自點(diǎn)形念珠藻PCC73102的NPcrtW酮酶基因，二者在每種情況下都是在三孢布拉霉pcarRA啟動子控制下(序列pBinAHygBTpcarRA-HPcrtZ-BTpcarRA-NpucrtW，SEQ.ID NO.51，圖19)。
對三孢布拉霉的遺傳修飾通過舉例的方式將所利用的基因和啟動子進(jìn)行克隆和序列分析多種三孢布拉霉基因和啟動子的克隆和測序通過以下舉例描述。
ptef1的克隆和測序分析三孢布拉霉p-tef是基于先前在GenBank發(fā)表的三孢布拉霉翻譯延伸因子1-α(AF157235)的結(jié)構(gòu)基因序列而克隆的。從序列入口AF157235出發(fā)，為了擴(kuò)增和測序所述結(jié)構(gòu)基因上游啟動子區(qū)域選擇了反向PCR引物。
在200ng的XhoI酶切的和環(huán)化的三孢布拉霉ATCC14272基因組DNA反向巢式PCR中，從以下反應(yīng)混合物中獲得了3000-bp片段模板DNA(1μg三孢布拉霉ATCC14272基因組DNA)、引物MAT3445’-GGCGTACTTGAAGGAACCCTTACCG-3’(SEQ.ID NO.63)和MAT3455’-ATTGATGCTCCCGGTCACCGTGATT-3’(SEQ.ID NO.64)，每種引物0.25μM、100μM dNTP，10μl 10×Herculase聚合酶緩沖液，5UHerculase(85℃時添加)，加H2O至100μl。PCR程序如下95℃，10分鐘(1個循環(huán))；85℃，5分鐘(1循環(huán))；60℃，30秒，72℃，60秒，95℃，30秒(30循環(huán))；72℃，10分鐘(1循環(huán))。在3000-bp片段中tef1基因預(yù)測的起始密碼子的上游序列片段稱作ptef1啟動子。
來自三孢布拉霉HMG-CoA還原酶基因的克隆和序列分析首先，用pANsCos1粘粒載體制備三孢布拉霉ATCC14272、(-)交配型的基因文庫。該載體經(jīng)XbaI酶切線性化和隨后去磷酸化。進(jìn)一步用BamHI酶切產(chǎn)生插入位點(diǎn)，與Sau3AI部分酶切并去磷酸化的三孢布拉霉基因組DNA相連接。該方法產(chǎn)生的粘粒隨后在體外經(jīng)包裝并轉(zhuǎn)移入大腸桿菌中。
基于編碼HMG-CoA還原酶的三孢布拉霉基因片段的已知序列(Eur.J.Biochem 220，403-408(1994))，從以下PCR反應(yīng)制備315-bp的DNA探針1μg三孢布拉霉ATCC14272基因組DNA、引物MAT3145’-CCGATGGCGACGACGGAAGGTTGTT-3’[SEQ.ID NO.79]和MAT315 5’-CATGTTCATGCCCATTGCATCACCT-3’[SEQ.IDNO.80]，每種引物0.25μM、100μM dNTP、10μl 10×Herculase聚合酶緩沖液、5U Herculase(在85℃添加)，加H2O至100μl。PCR程序如下95℃，10分鐘(1循環(huán))；85℃，5分鐘(1循環(huán))；58℃，30秒，72℃，30秒，95℃，30秒(30循環(huán))；72℃，10分鐘(1循環(huán))。
該DNA探針用于篩選粘粒基因庫。鑒定與所述探針雜交的粘?？寺?。測序粘粒的插入片段。該DNA序列包含屬于HMG-CoA還原酶基因的片段[HMG-CoA-Red.gb]。
carB的克隆和序列分析(carB＝三孢布拉霉八氫番茄紅素脫氫酶基因)簡并引物MAT182 5’-GCNGARGGNATHTGGTA-3’(SEQ.IDNO.52)和MAT192 5’-TCNGCNAGRAADATRTTRTG-3’(SEQ.IDNO.53)源自對布拉克須霉、煙草尾孢(Cercospora nicotianae)、法夫酵母(Phaffia rhodozyma)和粗糙脈孢菌的八氫番茄紅素脫氫酶多肽序列比對和相應(yīng)的DNA序列比對。PCR在100μl的反應(yīng)混合物中進(jìn)行。PCR反應(yīng)混合物中包含200ng三孢布拉霉ATCC14272基因組DNA、1μM MAT182、1μM MAT192、100μM dNTP、10μl 10×pfu聚合酶緩沖液、2.5U pfu聚合酶(在85℃添加)，加H2O至100μl。
PCR程序如下95℃，10分鐘(1循環(huán))；85℃，5分鐘(1循環(huán))；40℃，30秒，72℃，30秒，95℃，30秒(35循環(huán))；72℃，10分鐘(1循環(huán))。
這產(chǎn)生了導(dǎo)出的肽序列與八氫番茄紅素脫氫酶序列相似的358-bp的片段。反向PCR方法(Innis等，PCR protocolsa guide to methods andapplications.1990.pp.219-227)根據(jù)染色體步行的原理用于如下擴(kuò)增、克隆并測序350-bp片段上游和下游基因區(qū)域(i)用引物MAT219 5’-AAGTGACACCGGTTACACGCTTGTCTT-3’(SEQ.ID NO.54)和MAT 220 5’-GCTTATCACCATCTGTTACCTCCTTGC-3’(SEQ.ID NO.55)通過PCR，由200ng EcoRI酶切并環(huán)化的三孢布拉霉ATCC14272基因組DNA、0.25μM MAT219、0.25μMMAT220、100μM dNTP、10μl 10×Herculase聚合酶緩沖液、5U Herculase(在85℃添加)，加水至100μl獲得1.1kbp片段。PCR程序如下95℃，10分鐘(1循環(huán))；85℃，5分鐘(1循環(huán))；60℃，30秒，72℃，60秒，95℃，30秒(30循環(huán))；72℃，10分鐘(1循環(huán))。
(ii)引物MAT219和MAT220通過PCR，由200ng XbaI酶切并環(huán)化的三孢布拉霉ATCC14272基因組DNA、0.25μM MAT219、0.25μMMAT220、100μM dNTP、10μl 10×Herculase聚合酶緩沖液、5U Herculase(在85℃添加)，加水至100μl獲得2.9kbp片段。PCR程序如下95℃，10分鐘(1循環(huán))；85℃，5分鐘(1循環(huán))；60℃，30秒，72℃，3分鐘，95℃，30秒(30循環(huán))；72℃，10分鐘(1循環(huán))。
圖20概略地描述了克隆的序列片段。測序用克隆的片段和PCR產(chǎn)物在正義鏈和反義鏈方向進(jìn)行。圖21描述了克隆的序列片段的序列。
測序比對carB的核苷酸序列和衍生CarB蛋白的多肽序列與相關(guān)蛋白的已知序列比對。使用GAP和BESTFIT程序比對序列。
CarB-根據(jù)GAP一致的氨酰基殘基程序設(shè)置缺口權(quán)重 8長度權(quán)重 2平均匹配 2.912
平均不匹配 -2.003發(fā)現(xiàn)了以下與三孢布拉霉ATCC14272 CarB氨基酸以百分率表示的一致性值布拉克須霉 72.491法夫酵母 50.460粗糙脈孢菌 47.943煙草尾孢 47.740CarB-根據(jù)BESTFIT一致的氨?；绦蛟O(shè)置缺口權(quán)重 8長度權(quán)重 2平均匹配 2.912平均不匹配 -2.003發(fā)現(xiàn)了以下與三孢布拉霉ATCC14272 CarB氨基酸以百分率表示的一致性值布拉克須霉 73.380法夫酵母 53.175粗糙脈孢菌 51.896煙草尾孢 50.791carB-根據(jù)GAP一致的堿基程序設(shè)置缺口權(quán)重 50長度權(quán)重 3平均匹配 10.000Average Mismatch 0.000發(fā)現(xiàn)以下與三孢布拉霉ATCC14272 CarB堿基以百分率表示的一致性值布拉克須霉 64.853
煙草尾孢 50.143法夫酵母 43.179粗糙脈孢菌 42.130carB-根據(jù)BESTFIT一致的堿基缺口權(quán)重 50長度權(quán)重 3平均匹配 10.000平均不匹配 -9.000發(fā)現(xiàn)以下與三孢布拉霉ATCC14272 CarB堿基以百分率表示的一致性值布拉克須霉 68.926法夫酵母 62.403粗糙脈孢菌 60.230煙草尾孢 56.884carB表達(dá)的克隆為了克隆和表達(dá)三孢布拉霉carB，從三孢布拉霉上述克隆的序列片段的六個讀碼框中得到了可能的蛋白序列。這些蛋白序列與來自布拉克須霉，法夫酵母，粗糙脈孢菌，煙草尾孢八氫番茄紅素脫氫酶序列比對。在序列比對的基礎(chǔ)上，在三孢布拉霉基因組DNA的克隆序列片段中鑒定了三個外顯子，它們在一起形成了其基因產(chǎn)物全長與布拉克須霉CarB八氫番茄紅素脫氫酶有72.7％一致氨酰基殘基的編碼區(qū)。含有三個可能的外顯子和兩個可能的內(nèi)顯子的該序列片段因此稱作carB基因。為了檢查預(yù)測的基因結(jié)構(gòu)，通過PCR方法用三孢布拉霉cDNA作為模板以及Bol14255’-AGAGAGGGATCCTTAAATGCGAATATCGTTGC-3’(SEQ.IDNO.56)和Bol1426 5’-AGAGAGGGATCCATGTCTGATCAAAAGAAGCA-3’(SEQ.ID NO.57)引物產(chǎn)生了三孢布拉霉carB的編碼序列。對獲得的DNA片段測序。通過比對cDNA與基因組carB DNA，確證了外顯子和內(nèi)顯子的定位。圖21概略地描述了carB的編碼區(qū)。為了在大腸桿菌中表達(dá)carB，首先通過重疊延伸PCR方法去除carB中的NdeI酶切位點(diǎn)并在該基因5’末端引入Nde1酶切位點(diǎn)和在3’末端引入BamHI酶切位點(diǎn)。獲得的DNA片段與pJOE2702載體連接。獲得的質(zhì)粒稱作pBT4并與pCAR-AE一起克隆入大腸桿菌XL1-Blue。用鼠李糖誘導(dǎo)表達(dá)。通過HPLC檢測番茄紅素的方法檢測酶活性?？寺〔襟E描述如下PCR 1.1約0.5μg三孢布拉霉cDNA、0.25μM MAT3505’-ACTTTATTGGATCCTTAAATGCGAATATCGTTGCTGC-3’(SEQ.ID NO.58)、0.25μM MAT244 5’-GTTCCAATTGGCCACATGAAGAGTAAGACAGGAAACAG-3’(SEQ.ID NO.59)、100μM dNTP、10μl Pfu聚合酶緩沖液(10×)、2.5U Pfu聚合酶(85℃添加，″熱啟動″)和加水至100μL。
溫度程序1.95℃10分鐘，2.85℃5分鐘，3.40℃30秒，4.72℃1分鐘30秒，5.95℃30秒，6.50℃30秒，7.72℃1分鐘30秒，8.95℃30秒，9.72℃10分鐘循環(huán)(1-2.)1×，(3-5.)5×，(6-8.)25×，(9.)1×PCR 1.2約0.5μg三孢布拉霉cDNA、0.25μM MAT243 5’-CCTGTCTTACTCTTCATGTGGCCAATTGGAACCAACAC-3’(SEQ.ID NO.60)、0.25μM MAT353 5’-CTATTTTAATCATATGTCTGATCAAAAGAAGCATATTG-3’(SEQ.ID NO.61)、100μM dNTP、10μl Pfu聚合酶緩沖液(10×)、2.5U Pfu聚合酶(85℃添加，″熱啟動″)和加水至100μL。
溫度程序1.95℃10分鐘，2.85℃5分鐘，3.40℃30秒，4.72℃1分鐘30秒，5.95℃30秒，6.50℃30秒，7.72℃1分鐘30秒，8.95℃30秒，9.72℃10分鐘循環(huán)(1-2.)1×，(3-5.)5×，(6-8.)25×，(9.)1×從PCR 1.1、1.2中純化PCR片段為了該目的，進(jìn)行PCR 2以制備克隆入pJOE2702的三孢布拉霉carB編碼序列
約50ng PCR 1.1產(chǎn)物和約50ng PCR 1.2產(chǎn)物、與0.25μM MAT3505’-ACTTTATTGGATCCTTAAATGCGAATATCGTTGCTGC-3’(SEQ.ID NO.58)、0.25μM MAT353 5’-CTATTTTAATCATATGTCTGATCAAAAGAAGCATATTG-3’(SEQ.ID NO.61)、100μM dNTP、10μl Pfu聚合酶緩沖液(10×)、2.5U Pfu聚合酶(85℃添加，″熱啟動″)和加水至100μL。
溫度程序1.95℃10分鐘，2.85℃5分鐘，3.59℃30秒，4.72℃2分鐘30秒，5.95℃30秒，6.72℃10分鐘循環(huán)(1-2.)1×，(3-5.)22×，(6.)1×隨后，純化獲得的片段(～1.7kbp)，繼之以連接到pPCR-Script-Amp載體，克隆入大腸桿菌XL1-Blue，測序插入片段，用NdeI和BamHI酶切并連接入pJOE2702。獲得的質(zhì)粒稱作pBT4。
■鑒定性質(zhì)和檢測CarB(八氫番茄紅素脫氫酶)酶活性■自carB獲得的基因產(chǎn)物稱作CarB。CarB基于多肽序列分析具有以下性質(zhì)長度582個氨?；鶜埢肿恿? 66470等電點(diǎn) 6.7催化活性八氫番茄紅素脫氫酶反應(yīng)物 八氫番茄紅素產(chǎn)物番茄紅素EC號EC 1.14.99-在體內(nèi)檢測酶活性。將質(zhì)粒(pCAR-AE)轉(zhuǎn)入大腸桿菌XL1-Blue產(chǎn)生了大腸桿菌XL1-Blue(pCAR-AE)菌株。該菌株合成八氫番茄紅素。將另外的pBT4質(zhì)粒轉(zhuǎn)入大腸桿菌XL1-Blue產(chǎn)生了大腸桿菌XL1-Blue(pCAR-AE)(pBT4)菌株。由于自carB形成了具有酶活性的八氫番茄紅素脫氫酶，該菌株產(chǎn)生番茄紅素。
將質(zhì)粒pCAR-AE和pBT4因此轉(zhuǎn)入大腸桿菌中。從液體培養(yǎng)物中生長的細(xì)胞中提取類胡蘿卜素并鑒定性質(zhì)(見上文)。
HPLC分析揭示大腸桿菌XL1-Blue(pCAR-AE)菌株產(chǎn)生八氫番茄紅素以及大腸桿菌XL1-Blue(pCAR-AE)(pBT4)菌株產(chǎn)生番茄紅素。因此，CarB具有八氫番茄紅素脫氫酶的酶活性。
■產(chǎn)生八氫番茄紅素的遺傳修飾的三孢布拉霉菌株的制備■產(chǎn)生八氫番茄紅素的遺傳修飾的三孢布拉霉菌株的制備通過以下舉例的方式描述。
產(chǎn)生三孢布拉霉carB-突變體的pBinAHygΔcarB載體構(gòu)建pBinAHygΔcarB載體(SEQ.ID NO.62，圖22)用來在三孢布拉霉中刪除carB。pBinAHygΔcarB前體是如下構(gòu)建的pBinAHyg(SEQ.IDNO.3，圖2)gpdA-hph盒從pANsCos1(SEQ.ID.NO4，圖1，Osiewacz，1994，Curr.Genet.2687-90)質(zhì)粒作為BglII/HindIII片段分離并連接到BamHI/HindIII切開的pBin19(Bevan，1984，Nucleic Acids Res.128711-8721)雙元質(zhì)粒中。該方法獲得的質(zhì)粒稱作pBinAHyg并包含在來自構(gòu)巢曲霉的gpd啟動子控制下的大腸桿菌潮霉素抗性基因(hph)和trpC終止子以及農(nóng)桿菌DNA轉(zhuǎn)移所需要的合適邊界序列。
carB編碼序列是使用引物MAT350(SEQ.ID NO.58)和MAT353(SEQ.ID NO.61)以及以下參數(shù)通過PCR方法擴(kuò)增的50ng pBT4與0.25μM MAT350 5’-ACTTTATTGGATCCTTAAATGCGAATATCGTTGCTGC-3’、0.25μM MAT353 5’-CTATTTTAATCATATGTCTGATCAAAAGAAGCATATTG-3’、100μM dNTP、10μlPfu聚合酶緩沖液、2.5U Pfu聚合酶(85℃添加，″熱啟動″)并加水至100μL。
溫度程序1.95℃10分鐘，2.85℃5分鐘，3.58℃30秒，4.72℃2分鐘，5.95℃30秒，6.72℃10分鐘循環(huán)(1-2.)1×，(3-5.)30×，(6.)1×純化獲得的片段(～1.7kbp)，繼之以用HindIII酶切，進(jìn)一步純化364bp HindIII carB片段，繼之以用HindIII切割pBinAHyg，將364bp的HindIII carB片段連接入pBinAHyg，轉(zhuǎn)化該載體于大腸桿菌中并分離構(gòu)建體，且如上所述稱作pBinAHygΔcarB。備選地，用HindIII部分酶切并將較大的carBHindIII片段克隆入pBinAHyg產(chǎn)生pBinAHygΔcarB。
三孢布拉霉carB-突變體的產(chǎn)生首先將pBinAHygΔcarB質(zhì)粒如通過電穿孔(見上文)轉(zhuǎn)入農(nóng)桿菌菌株LBA 4404。隨后將該質(zhì)粒從根癌農(nóng)桿菌LBA 4404轉(zhuǎn)入三孢布拉霉ATCC14271和三孢布拉霉ATCC14272(見上文)。根據(jù)以下步驟通過聚合酶鏈?zhǔn)椒磻?yīng)成功檢測基因轉(zhuǎn)入三孢布拉霉約0.5μg來自三孢布拉霉ATCC14272 carB-或三孢布拉霉ATCC14271 carB-DNA與0.25μM hph正向引物5’-CGATGTAGGAGGGCGTGGATA-3’(SEQ.ID NO.5)、0.25μM hph反向引物5’-GCTTCTGCGGGCGATTTGTGT-3’(SEQ.ID NO.6)、100μM dNTP、10μl Herculase聚合酶緩沖液、2.5U Herculase DNA聚合酶(85℃添加，″熱啟動″)和加H2O至100μL反應(yīng)。
溫度程序1.95℃10分鐘，2.85℃5分鐘，3.58℃1分鐘，4.72℃1分鐘，5.94℃1分鐘，6.72℃10分鐘循環(huán)(1-2.)1×，(3-5.)30×，(6.)1×嘗試擴(kuò)增農(nóng)桿菌卡那霉素抗性基因作為陰性對照。為了該目的，使用以下PCR條件約0.5μg來自三孢布拉霉ATCC14272 carB-和三孢布拉霉ATCC14271 carB-DNA與0.25μM nptIII正向引物5’-TGAGAATATCACCGGAATTG-3’(SEQ.ID NO.7)，0.25μM nptIII反向引物5’-AGCTCGACATACTGTTCTTCC-3’(SEQ.ID NO.8)、100μM dNTP、10μl Herculase聚合酶緩沖液、2.5U Herculase DNA聚合酶(85℃添加，″熱啟動″)和加H2O至100μL反應(yīng)。
溫度程序1.95℃10分鐘，2.85℃5分鐘，3.58℃1分鐘，4.72℃1分鐘，5.94℃1分鐘，6.72℃10分鐘循環(huán)(1-2.)1×，(3-5.)30×，(6.)1×C)由三孢布拉霉產(chǎn)生類胡蘿卜素和類胡蘿卜素前體類胡蘿卜素玉米黃素、角黃素、蝦青素和八氫番茄紅素是通過發(fā)酵相應(yīng)的遺傳修飾的三孢布拉霉(+)和(-)菌株產(chǎn)生的，通過HPLC分析檢測產(chǎn)生的類胡蘿卜素并分離之。
用于產(chǎn)生類胡蘿卜素的液體培養(yǎng)基每升包含19g玉米粉、44g大豆粉、0.55g KH2PO4、0.002g鹽酸硫胺素、10％向日葵油。用KOH調(diào)pH至7.5。
為了產(chǎn)生類胡蘿卜素，將搖瓶接種以遺傳修飾的三孢布拉霉(+)和(-)菌株的孢子懸液。搖瓶在26℃、250轉(zhuǎn)/分鐘培養(yǎng)7天。備選地，四天后向菌株混合液中加入三孢酸，繼續(xù)培養(yǎng)3天。三孢酸的終濃度為300-400μg/ml。
提取和分析提取1.取出10ml培養(yǎng)物懸液2.5000×g離心10分鐘3.棄上清4.通過渦旋將沉淀重懸于1ml四氫呋喃(THF)中5.5000×g離心5分鐘6.取出THF相7.重復(fù)步驟4.-6.(2次)8.合并THF相9.將合并的THF相20000×g離心5分鐘以去除剩余的水相。
分析通過HPLC測定八氫番茄紅素柱ZORBAX Eclipse XDB-C8，5μm，150*4.6mm溫度 40℃流速 0.5ml/分鐘注射體積 10μl檢測 UV 220nm停止時間 12分鐘后運(yùn)行時間(Post run time)0分鐘最大壓力 350巴洗脫液A 含高氯酸的50mM NaH2PO4，pH2.5洗脫液B 乙腈梯度時間[分鐘] A[％]B[％]流速[ml/分鐘]0 50 50 0.512 50 50 0.5將發(fā)酵肉湯的提取物用作基質(zhì)。HPLC之前，每個樣品經(jīng)0.22μm的濾膜過濾。樣品要保持冰冷并避光。在每種情況下稱取50-1000mg/l并溶于THF用于校準(zhǔn)。使用的標(biāo)準(zhǔn)品是八氫番茄紅素，它在給定的條件下保留時間為7.7分鐘。
通過HPLC測定番茄紅素、β-胡蘿卜素、海膽酮、角黃素、隱黃素、玉米黃素和蝦青素柱Nucleosil 100-7C18，250*4.0mm(Macherey & Nagel)溫度 25℃流速 1.3ml/分鐘注射體積 10μl檢測 450nm停止時間 15分鐘后運(yùn)行時間 2分鐘最大壓力 250巴洗脫液A10％丙酮，90％H2O洗脫液B丙酮梯度時間[分鐘] A[％]B[％]流速[ml/分鐘]0 30 70 1.310 595 1.312 595 1.313 30 70 1.3將發(fā)酵肉湯的提取物用作基質(zhì)。HPLC之前，每個樣品經(jīng)0.22μm的濾膜過濾。樣品要保持冰冷并避光。在每種情況下稱取10mg并溶于100mlTHF用于校準(zhǔn)。具有以下保留時間的以下類胡蘿卜素用作標(biāo)準(zhǔn)品β-胡蘿卜素(12.5分鐘)、番茄紅素(11.7分鐘)、海膽酮(10.9分鐘)、隱黃素(10.5分鐘)、角黃素(8.7分鐘)、玉米黃素(7.6分鐘)和蝦青素(6.4分鐘)[見圖23]。
通過遺傳修飾的三孢布拉霉產(chǎn)生玉米黃素通過遺傳修飾的三孢布拉霉產(chǎn)生玉米黃素由以下例子描述。
通過農(nóng)桿菌介導(dǎo)的轉(zhuǎn)化將pBinAHygBTpTEF1-HPcrtZ質(zhì)粒轉(zhuǎn)入三孢布拉霉中(見上文)。分離潮霉素抗性克隆并轉(zhuǎn)到馬鈴薯-葡萄糖瓊脂平板上(Merck KGaA，Darmstadt，Germany)。
該平板于26℃培養(yǎng)三天后制備孢子懸液。于含有50ml生長培養(yǎng)基(47g/l玉米粉、23g/l大豆粉、0.5g/l KH2PO4、2.0mg/l鹽酸硫胺素，滅菌前用NaOH調(diào)pH至6.2-6.7)的250ml無擋板錐形瓶中接種1×105孢子。該預(yù)培養(yǎng)物于26℃、250轉(zhuǎn)/分鐘培養(yǎng)48小時。對于主培養(yǎng)，于含有40ml生產(chǎn)培養(yǎng)基的250ml無擋板錐形瓶中接種4ml預(yù)培養(yǎng)物并在26℃、150轉(zhuǎn)/分鐘培養(yǎng)8天。生產(chǎn)培養(yǎng)包含50g/l葡萄糖、2g/l酪蛋白酸水解物、1g/l酵母提取物、2g/l L-天冬酰胺、1.5g/lK H2PO4、0.5g/l MgSO4×7H2O、5mg/l鹽酸硫胺素、10g/l Span20、1g/l吐溫80、20g/l亞油酸、80g/l玉米漿。72小時后，加入煤油至終濃度40g/l。收集培養(yǎng)物后，用水將剩余約為35ml的培養(yǎng)物體積增加至40ml。隨后，在高壓勻漿器type MicronLab 40，APV Gaulin內(nèi)，1500巴3次將細(xì)胞破裂。
含有經(jīng)破裂細(xì)胞的懸液與35ml THF混合并在黑暗中250轉(zhuǎn)/分鐘室溫?fù)u動孵育60分鐘。然后加入2g NaCl并再次搖動孵育該混合物。然后5000×g離心提取混合物10分鐘。取出有色的THF相，且細(xì)胞塊是完全無色的。
將THF相在旋轉(zhuǎn)蒸發(fā)器內(nèi)于30毫巴、30℃濃縮至1ml并再次置于1ml THF中。20000×g離心5分鐘后，取一部分上層相并用HPLC分析(圖24、圖23)。
D)加工和分離類胡蘿卜素和食品為了獲得高純度的類胡蘿卜素和相應(yīng)的食品，在A)的上述培養(yǎng)肉湯得到如下加工。
培養(yǎng)肉湯1、2、3的類胡蘿卜素含量在0.5和1.5g/l之間。
■D1)根據(jù)方案a)IIA和方案b)IIA或IIB的實(shí)例具有相同培養(yǎng)基的培養(yǎng)物(共約1升)在培養(yǎng)末期合并而且使用分散劑(Ultra.Turrax_)勻漿。
培養(yǎng)基1和2中的固體濃度分別為37g/l和11g/l。培養(yǎng)肉湯用離心脫水。如果培養(yǎng)基的細(xì)胞濃度和固體含量高，培養(yǎng)肉湯也可以進(jìn)一步加工而不需先前的固體-液體分離(培養(yǎng)基3127g固體/l)。在前述用分散劑(Ultra-Turrax_)勻漿并持續(xù)攪拌懸浮液后，細(xì)胞塊通過蠕動泵導(dǎo)入干燥器。通過直徑為2.0mm的由兩個成分組成的管口以2巴和4.5Nm3/h氮?dú)庾⑷雽?shí)驗室噴霧干燥器的圓柱體內(nèi)室。進(jìn)口溫度約為125℃至127℃。干燥氣體為流速為22Nm3/h的氮?dú)?。出口溫度?9℃和61℃之間。對于三種發(fā)酵肉湯的每一種，可以使可流動的產(chǎn)品沉淀至噴霧干燥器的旋風(fēng)分離器上。室內(nèi)的壁膜自動地從容器壁脫落并很容易分開。
得到8到100g可直接用作動物飼料的粉狀食品。它包含約1-10％基于干重的類胡蘿卜素。殘留水分小于5％。
根據(jù)方案b)IIC的實(shí)例■D2)用四氫呋喃提取在每種情況下40ml培養(yǎng)肉湯1、2、3中的細(xì)胞在type Micron Lab 40，APV Gaulin高壓勻漿器中以1500巴破壞三次。在每種情況下含有破壞細(xì)胞的20ml懸液與20ml四氫呋喃混合并于旋轉(zhuǎn)搖床中30℃、200轉(zhuǎn)/分鐘振蕩孵育30分鐘。然后加入2g NaCl并通過5000×g離心5分鐘分離不同相。取出THF相。隨后，水相用20ml THF再次提取。合并提取物。類胡蘿卜素濃度通過HPLC定量。
■D3)用二氯甲烷提取在實(shí)驗室離心機(jī)通過5000×g離心10分鐘從培養(yǎng)肉湯(200ml)中去除生物量。
去除的濕生物量(在每種情況下約10g到100g)與10-100ml水混合以去除水溶性成分。將生物量取出(實(shí)驗室離心機(jī))并然后在高壓滅菌鍋中用蒸汽滅菌(溫度＝121，時間＝30分鐘，1巴)以破壞細(xì)胞。
將25-250g二氯甲烷加入細(xì)胞碎片中并通過振蕩從生物量中提取類胡蘿卜素。生物量在實(shí)驗室離心機(jī)中去除。
從二氯甲烷到甲醇進(jìn)行溶液置換，類胡蘿卜素溶液保持在40℃到60℃約4小時且在此過程中不斷地與總體積20-200ml的甲醇混合。二氯甲烷作為溶劑在過程中得到回收。首先，沉淀類胡蘿卜素晶體。然后，隨著類胡蘿卜素晶體的大小和數(shù)量不斷增加，將溶液在6小時內(nèi)緩慢降溫至約10℃。然后過濾母液并干燥類胡蘿卜素晶體。部分的母液可以再次用于溶液置換。其余的部分經(jīng)蒸餾且該方法純化的甲醇再次用于溶液置換。
獲得了純度(HPLC，參見上文)為95％的0.008g至0.24g類胡蘿卜素晶體?；谏锪恐蓄惡}卜素濃度，類胡蘿卜素晶體的產(chǎn)量為80％。
在蒸汽蒸餾后去除二氯甲烷的濕的生物量經(jīng)噴霧干燥(TE＝125℃、TA＝60℃)并可用作動物飼料添加劑。
為此目的，在前述用分散劑(Ultra-Turrax)勻漿并持續(xù)攪拌懸浮液后，細(xì)胞量通過蠕動泵導(dǎo)入干燥器。
通過直徑為2.0mm的由兩個成分組成的管口以2巴和4.5Nm3/h氮?dú)庾⑷雽?shí)驗室噴霧干燥器的圓柱體內(nèi)室。進(jìn)口溫度約為125℃至127℃。干燥氣體為流速為22Nm3/h的氮?dú)?。出口溫度?9℃和61℃之間。對于三種發(fā)酵肉湯的每一種，可以使可流動的產(chǎn)品沉淀至噴霧干燥器的旋風(fēng)分離器上。室內(nèi)的壁膜(如果存在)自動地從容器壁脫落并很容易分開。
得到約2.5-25g可直接用作動物飼料的粉狀食品。它包含約0.5-1.5％基于干重的類胡蘿卜素。殘留水分小于5％。
基于培養(yǎng)肉湯中類胡蘿卜素的起始量，類胡蘿卜素的總產(chǎn)量約為95％(包括純化的類胡蘿卜素食品)。
序列表<110>巴斯福股份公司<120>使用經(jīng)遺傳修飾的布拉霉屬生物產(chǎn)生類胡蘿卜素或其前體的方法、由所述方法產(chǎn)生的類胡蘿卜素或其前體及它們的用途<130>BASF/NAE877/03<160>80<170>PatentIn版本3.2<210>1<211>2160<212>DNA<213>人工序列<220>
<223>啟動子<400>1ctttcgacac tgaaatacgt cgagcctgct ccgcttggaa gcggcgagga gcctcgtcct 60gtcacaacta ccaacatgga gtacgataag ggccagttcc gccagctcat taagagccag120ttcatgggcg ttggcatgat ggccgtcatg catctgtact tcaagtacac caacgctctt180ctgatccagt cgatcatccg ctgaaggcgc tttcgaatct ggttaagatc cacgtcttcg240ggaagccagc gactggtgac ctccagcgtc cctttaaggc tgccaacagc tttctcagcc300agggccagcc caagaccgac aaggcctccc tccagaacgc cgagaagaac tggaggggtg360gtgtcaagga ggagtaagct ccttattgaa gtcggaggac ggagcggtgt caagaggata420ttcttcgact ctgtattata gataagatga tgaggaattg gaggtagcat agcttcattt480ggatttgctt tccaggctga gactctagct tggagcatag agggtccttt ggctttcaat540attctcaagt atctcgagtt tgaacttatt ccctgtgaac cttttattca ccaatgagca600ttggaatgaa catgaatctg aggactgcaa tcgccatgag gttttcgaaa tacatccgga660
tgtcgaaggc ttggggcacc tgcgttggtt gaatttagaa cgtggcacta ttgatcatcc720gatagctctg caaagggcgt tgcacaatgc aagtcaaacg ttgctagcag ttccaggtgg780aatgttatga tgagcattgt attaaatcag gagatatagc atgatctcta gttagctcac840cacaaaagtc agacggcgta accaaaagtc acacaacaca agctgtaagg atttcggcac900ggctacggaa gacggagaag ccaccttcag tggactcgag taccatttaa ttctatttgt960gtttgatcga gacctaatac agcccctaca acgaccatca aagtcgtata gctaccagtg 1020aggaagtgga ctcaaatcga cttcagcaac atctcctgga taaactttaa gcctaaacta 1080tacagaataa gataggtgga gagcttatac cgagctccca aatctgtcca gatcatggtt 1140gaccggtgcc tggatcttcc tatagaatca tccttattcg ttgacctagc tgattctgga 1200gtgacccaga gggtcatgac ttgagcctaa aatccgccgc ctccaccatt tgtagaaaaa 1260tgtgacgaac tcgtgagctc tgtacagtga ccggtgactc tttctggcat gcggagagac 1320ggacggacgc agagagaagg gctgagtaat aagccactgg ccagacagct ctggcggctc 1380tgaggtgcag tggatgatta ttaatccggg accggccgcc cctccgcccc gaagtggaaa 1440ggctggtgtg cccctcgttg accaagaatc tattgcatca tcggagaata tggagcttca 1500tcgaatcacc ggcagtaagc gaaggagaat gtgaagccag gggtgtatag ccgtcggcga 1560aatagcatgc cattaaccta ggtacagaag tccaattgct tccgatctgg taaaagattc 1620acgagatagt accttctccg aagtaggtag agcgagtacc cggcgcgtaa gctccctaat 1680tggcccatcc ggcatctgta gggcgtccaa atatcgtgcc tctcctgctt tgcccggtgt 1740atgaaaccgg aaaggccgct caggagctgg ccagcggcgc agaccgggaa cacaagctgg 1800cagtcgaccc atccggtgct ctgcactcga cctgctgagg tccctcagtc cctggtaggc 1860agctttgccc cgtctgtccg cccggtgtgt cggcggggtt gacaaggtcg ttgcgtcagt 1920ccaacatttg ttgccatatt ttcctgctct ccccaccagc tgctcttttc ttttctcttt 1980
cttttcccat cttcagtata ttcatcttcc catccaagaa cctttatttc ccctaagtaa 2040gtactttgct acatccatac tccatccttc ccatccctta ttcctttgaa cctttcagtt 2100cgagctttcc cacttcatcg cagcttgact aacagctacc ccgcttgagc agacatcacc 2160<210>2<211>774<212>DNA<213>人工序列<220>
<223>終止子<220>
<221>misc_feature<222>(267)..(267)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(475)..(475)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(566)..(566)<223>n為a，c，g，或t<400>2cgatccactt aacgttactg aaatcatcaa acagcttgac gaatctggat ataagatcgt 60tggtgtcgat gtcagctccg gagttgagac aaatggtgtt caggatctcg ataagatacg 120ttcatttgtc caagcagcaa agagtgcctt ctagtgattt aatagctcca tgtcaacaag 180aataaaacgc gttttcgggt ttacctcttc cagatacagc tcatctgcaa tgcattaatg 240cattgactgc aacctagtaa cgccttncag gctccggcga agagaagaat agcttagcag 300agctattttc attttcggga gacgagatca agcagatcaa cggtcgtcaa gagacctacg 360
agactgagga atccgctctt ggctccacgc gactatatat ttgtctctaa ttgtactttg420acatgctcct cttctttact ctgatagctt gactatgaaa attccgtcac cagcncctgg480gttcgcaaag ataattgcat gtttcttcct tgaactctca agcctacagg acacacattc540atcgtaggta taaacctcga aatcanttcc tactaagatg gtatacaata gtaaccatgc600atggttgcct agtgaatgct ccgtaacacc caatacgccg gccgaaactt ttttacaact660ctcctatgag tcgtttaccc agaatgcaca ggtacacttg tttagaggta atccttcttt720ctagctagaa gtcctcgtgt actgtgtaag cgcccactcc acatctccac tcga 774<210>3<211>15739<212>DNA<213>人工序列<220>
<223>載體<220>
<221>misc_feature<222>(347)..(3471)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3679)..(3679)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3770)..(3770)<223>n為a，c，g，或t<400>3gatctttcga cactgaaata cgtcgagcct gctccgcttg gaagcggcga ggagcctcgt 60cctgtcacaa ctaccaacat ggagtacgat aagggccagt tccgccagct cattaagagc120
cagttcatgg gcgttggcat gatggccgtc atgcatctgt acttcaagta caccaacgct180cttctgatcc agtcgatcat ccgctgaagg cgctttcgaa tctggttaag atccacgtct240tcgggaagcc agcgactggt gacctccagc gtccctttaa ggctgccaac agctttctca300gccagggcca gcccaagacc gacaaggcct ccctccagaa cgccgagaag aactggaggg360gtggtgtcaa ggaggagtaa gctccttatt gaagtcggag gacggagcgg tgtcaagagg420atattcttcg actctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca480tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc tttggctttc540aatattctca agtatctcga gtttgaactt attccctgtg aaccttttat tcaccaatga600gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc660ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca720tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg780tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct840caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg900cacggctacg gaagacggag aagccacctt cagtggactc gagtaccatt taattctatt960tgtgtttgat cgagacctaa tacagcccct acaacgacca tcaaagtcgt atagctacca 1020gtgaggaagt ggactcaaat cgacttcagc aacatctcct ggataaactt taagcctaaa 1080ctatacagaa taagataggt ggagagctta taccgagctc ccaaatctgt ccagatcatg 1140gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct 1200ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa 1260aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga 1320gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca gctctggcgg 1380ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc cccgaagtgg 1440
aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga atatggagct 1500tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta tagccgtcgg 1560cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc tggtaaaaga 1620ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg taagctccct 1680aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg 1740tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc 1800tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta 1860ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc 1920agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc 1980tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag 2040taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca 2100gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc 2160accatgcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 2220gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 2280ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 2340tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 2400gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 2460gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 2520atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 2580ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 2640tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 2700atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 2760
aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 2820ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 2880atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 2940ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 3000aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 3060gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 3120gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 3180tagagtagat gccgaccgcg ggatcgatcc acttaacgtt actgaaatca tcaaacagct 3240tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg 3300tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg 3360atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata 3420cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg 3480gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga 3540tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat 3600atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat 3660gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact 3720ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa 3780gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac 3840gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca 3900cttgtttaga ggtaatcctt ctttctagct agaagtcctc gtgtactgtg taagcgccca 3960ctccacatct ccactcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt 4020ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 4080
agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 4140tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 4200cggggagagg cggtttgcgt attgggccaa agacaaaagg gcgacattca accgattgag 4260ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca ccgtcaccga 4320cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta ccattagcaa 4380ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta gcgacagaat 4440caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg gtcatagccc 4500ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag ccaccaccgg 4560aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca ccaccctcag 4620agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggc ccgatctagt 4680aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct 4740atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac 4800gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata 4860atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa 4920cgatcgggga tcatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag 4980atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg 5040ccgggcccga tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc 5100gtaatgatat cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc 5160agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag 5220cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc 5280gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat 5340agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag 5400
cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc 5460ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca 5520tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg tcgggcatgc 5580gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat 5640catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg 5700cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag 5760ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca 5820cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc 5880aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca 5940gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga 6000acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct 6060ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc 6120cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt ggataccgag 6180gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg 6240accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa 6300actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg 6360taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc 6420tcatgatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata 6480ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 6540gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga 6600tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc 6660gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca gaatgccata 6720
gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat 6780aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtat 6840gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt 6900ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg 6960acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc 7020gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt 7080tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg 7140gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg 7200aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc 7260ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc 7320gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact 7380gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg cggcggcacc 7440gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc 7500ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg 7560aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc aggaccgctg 7620ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc 7680accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc 7740aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc gcttcctcgc 7800tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7860cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7920gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7980gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 8040
gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 8100ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgcttttcc 8160gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc 8220gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg 8280cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact 8340gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct 8400accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag 8460ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag 8520gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa 8580atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg 8640ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc 8700ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc 8760aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa 8820cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga 8880cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc gcctttgcga 8940cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg 9000cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc 9060tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg 9120actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga 9180gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga 9240tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg 9300acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc 9360
acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc9420gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa9480accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg9540cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg9600cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt ccttgccatt9660gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc cggaagcatt9720gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag tgagggcggc9780ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga cttcatggcg9840gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc cgtgctcgtg9900ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt9960atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct 10020accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata 10080ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga tttcaggggg 10140caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg 10200catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat 10260tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg 10320tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc 10380tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc 10440agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg 10500tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat 10560acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc 10620gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg 10680
cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg 10740tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca 10800tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc 10860atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac 10920gcaccacccc gtcagtagct gaacaggagg gacagctgat agacacagaa gccactggag 10980cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cataattgtg 11040gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa 11100aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct 11160tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata 11220aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt 11280aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa 11340cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga 11400aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga 11460acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga 11520agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag 11580gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt 11640agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga 11700agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc 11760cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga 11820tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga 11880cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct 11940attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact 12000
ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag caggagcgca 12060ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg 12120gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg 12180gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg 12240caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc 12300cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg 12360acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc 12420cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc 12480gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt 12540cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag 12600gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg 12660aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt 12720ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc 12780gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg 12840tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg 12900acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg 12960agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc 13020ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca 13080cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg 13140accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt 13200ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg 13260cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa 13320
ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg 13380gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg 13440acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag 13500ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact tgcttcgctc 13560agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag gattaaaatt 13620gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc aggatttccg 13680cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg tttacgagca 13740cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg tggcattcgg 13800cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg acggccccaa 13860ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc gaggccgagg 13920ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg 13980acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc 14040gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac 14100ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc 14160gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt 14220gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt 14280ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac 14340ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt 14400tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct 14460gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac 14520agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc cggggatgca 14580tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag caatggatag 14640
gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc ttcctcagcg 14700gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca gcctgtcacg 14760gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg agatgatatt 14820tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc 14880atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga 14940gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc 15000ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg 15060gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga 15120cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg 15180cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc 15240aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat aaatcaaaag 15300aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 15360acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 15420aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 15480ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 15540aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg ggaagggcga 15600tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 15660ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa 15720ttcgagctcg gtacccggg 15739<210>4<211>11611<212>DNA<213>人工序列
<220>
<223>載體<220>
<221>misc_feature<222>(227)..(227)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(318)..(318)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(526)..(526)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(8946)..(8946)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10028)..(10028)<223>n為a，c，g，或t<400>4agcttgcatg cctgcaggtc gagtggagat gtggagtggg cgcttacaca gtacacgagg60acttctagct agaaagaagg attacctcta aacaagtgta cctgtgcatt ctgggtaaac 120gactcatagg agagttgtaa aaaagtttcg gccggcgtat tgggtgttac ggagcattca 180ctaggcaacc atgcatggtt actattgtat accatcttag taggaantga tttcgaggtt 240tatacctacg atgaatgtgt gtcctgtagg cttgagagtt caaggaagaa acatgcaatt 300atctttgcga acccaggngc tggtgacgga attttcatag tcaagctatc agagtaaaga 360agaggagcat gtcaaagtac aattagagac aaatatatag tcgcgtggag ccaagagcgg 420
attcctcagt ctcgtaggtc tcttgacgac cgttgatctg cttgatctcg tctcccgaaa480atgaaaatag ctctgctaag ctattcttct cttcgccgga gcctgnaagg cgttactagg540ttgcagtcaa tgcattaatg cattgcagat gagctgtatc tggaagaggt aaacccgaaa600acgcgtttta ttcttgttga catggagcta ttaaatcact agaaggcact ctttgctgct660tggacaaatg aacgtatctt atcgagatcc tgaacaccat ttgtctcaac tccggagctg720acatcgacac caacgatctt atatccagat tcgtcaagct gtttgatgat ttcagtaacg780ttaagtggat cgatcccgcg gtcggcatct actctattcc tttgccctcg gacgagtgct840ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc900gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc960gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag 1020ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg 1080atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa 1140gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac 1200cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag 1260gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac 1320ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat 1380cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc 1440gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg 1500ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg 1560gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat 1620cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc 1680gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga 1740
ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa 1800cttctcgaca gacgtcgcgg tgagttcagg catggtgatg tctgctcaag cggggtagct 1860gttagtcaag ctgcgatgaa gtgggaaagc tcgaactgaa aggttcaaag gaataaggga 1920tgggaaggat ggagtatgga tgtagcaaag tacttactta ggggaaataa aggttcttgg 1980atgggaagat gaatatactg aagatgggaa aagaaagaga aaagaaaaga gcagctggtg 2040gggagagcag gaaaatatgg caacaaatgt tggactgacg caacgacctt gtcaaccccg 2100ccgacacacc gggcggacag acggggcaaa gctgcctacc agggactgag ggacctcagc 2160aggtcgagtg cagagcaccg gatgggtcga ctgccagctt gtgttcccgg tctgcgccgc 2220tggccagctc ctgagcggcc tttccggttt catacaccgg gcaaagcagg agaggcacga 2280tatttggacg ccctacagat gccggatggg ccaattaggg agcttacgcg ccgggtactc 2340gctctaccta cttcggagaa ggtactatct cgtgaatctt ttaccagatc ggaagcaatt 2400ggacttctgt acctaggtta atggcatgct atttcgccga cggctataca cccctggctt 2460cacattctcc ttcgcttact gccggtgatt cgatgaagct ccatattctc cgatgatgca 2520atagattctt ggtcaacgag gggcacacca gcctttccac ttcggggcgg aggggcggcc 2580ggtcccggat taataatcat ccactgcacc tcagagccgc cagagctgtc tggccagtgg 2640cttattactc agcccttctc tctgcgtccg tccgtctctc cgcatgccag aaagagtcac 2700cggtcactgt acagagctca cgagttcgtc acatttttct acaaatggtg gaggcggcgg 2760attttaggct caagtcatga ccctctgggt cactccagaa tcagctaggt caacgaataa 2820ggatgattct ataggaagat ccaggcaccg gtcaaccatg atctggacag atttgggagc 2880tcggtataag ctctccacct atcttattct gtatagttta ggcttaaagt ttatccagga 2940gatgttgctg aagtcgattt gagtccactt cctcactggt agctatacga ctttgatggt 3000cgttgtaggg gctgtattag gtctcgatca aacacaaata gaattaaatg gtactcgagt 3060
ccactgaagg tggcttctcc gtcttccgta gccgtgccga aatccttaca gcttgtgttg 3120tgtgactttt ggttacgccg tctgactttt gtggtgagct aactagagat catgctatat 3180ctcctgattt aatacaatgc tcatcataac attccacctg gaactgctag caacgtttga 3240cttgcattgt gcaacgccct ttgcagagct atcggatgat caatagtgcc acgttctaaa 3300ttcaaccaac gcaggtgccc caagccttcg acatccggat gtatttcgaa aacctcatgg 3360cgattgcagt cctcagattc atgttcattc caatgctcat tggtgaataa aaggttcaca 3420gggaataagt tcaaactcga gatacttgag aatattgaaa gccaaaggac cctctatgct 3480ccaagctaga gtctcagcct ggaaagcaaa tccaaatgaa gctatgctac ctccaattcc 3540tcatcatctt atctataata cagagtcgaa gaatatcctc ttgacaccgc tccgtcctcc 3600gacttcaata aggagcttac tcctccttga caccacccct ccagttcttc tcggcgttct 3660ggagggaggc cttgtcggtc ttgggctggc cctggctgag aaagctgttg gcagccttaa 3720agggacgctg gaggtcacca gtcgctggct tcccgaagac gtggatctta accagattcg 3780aaagcgcctt cagcggatga tcgactggat cagaagagcg ttggtgtact tgaagtacag 3840atgcatgacg gccatcatgc caacgcccat gaactggctc ttaatgagct ggcggaactg 3900gcccttatcg tactccatgt tggtagttgt gacaggacga ggctcctcgc cgcttccaag 3960cggagcaggc tcgacgtatt tcagtgtcga aagatctgat caagagacag gatgaggatc 4020gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 4080gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 4140gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 4200tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 4260agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 4320ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 4380
tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 4440acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 4500ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 4560gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 4620ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 4680tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4740ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4800ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 4860cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4920ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4980ttcttcgccc accccgggct cgatcccctc gcgagttggt tcagctgctg cctgaggctg 5040gacgacctcg cggagttcta ccggcagtgc aaatccgtcg gcatccagga aaccagcagc 5100ggctatccgc gcatccatgc ccccgaactg caggagtggg gaggcacgat ggccgctttg 5160gtccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct 5220acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta 5280ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag 5340tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat 5400gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa 5460gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat 5520agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag 5580aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac 5640atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa 5700
aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat 5760agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt 5820aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 5880taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5940aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 6000ttatcatgtc tggatctgac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct 6060aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg 6120aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt 6180ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg 6240actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6300tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6360aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6420gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6480gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6540tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6600ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6660ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6720tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6780tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6840ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6900aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 6960ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7020
tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7080atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7140aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7200ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7260tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7320ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7380tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7440aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt 7500gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7560tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7620cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7680tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7740ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac 7800cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7860actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7920ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7980aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8040ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8100atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8160tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8220gccctttcgt cttcaagaat tcgcggccgc aattaaccct cactaaagga tccctatagt 8280gagtcgtatt atgcggccgc gaattctcat gtttgaccgc ttatcatcga taagctctgc 8340
tttttgttga cttccattgt tcattccacg gacaaaaaca gagaaaggaa acgacagagg 8400ccaaaaagct cgctttcagc acctgtcgtt tcctttcttt tcagagggta ttttaaataa 8460aaacattaag ttatgacgaa gaagaacgga aacgccttaa accggaaaat tttcataaat 8520agcgaaaacc cgcgaggtcg ccgccccgta acaaggcgga tcgccggaaa ggacccgcaa 8580atgataataa ttatcaattg catactatcg acggcactgc tgccagataa caccaccggg 8640gaaacattcc atcatgatgg ccgtgcggac ataggaagcc agttcatcca tcgctttctt 8700gtctgctgcc atttgctttg tgacatccag cgccgcacat tcagcagcgt ttttcagcgc 8760gttttcgatc aacgtttcaa tgttggtatc aacaccaggt ttaactttga acttatcggc 8820actgacggtt accttgttct gcgctggctc atcacgcagg ataccaaggc tgatgttgta 8880gatattggtc accggctgag ggttttcgat tgccgctgcg tggatagcac catttgcgat 8940caggcngtcc ttgatgaatg acactccatt gcgaataagt tcgaaggaga cggtgtcacg 9000aatgcgctgg tccagctcgg tcgattgcct tttgtgcagc agaggtatca atctcaacgc 9060caaggctcat cgaagcgcaa tattgctgct caccaaaacg cgtattgacc aggtgttcaa 9120cggcaaattt ctgcccttct gatgtcagaa aggcaaagtg attttctttc tggtattcag 9180ttgctgtgtg tcggtttcag caaaaccaag ctcgcgcaat tcggctgtgc agatttagaa 9240ggcagatcac cagacagcaa cggccaacgg aaaacagcgc atacagaaca tccgtcgccg 9300cgccgacaac gtgataattt ttatgaccca tgatttattt ccttttagac gtgagcctgt 9360cgcacagcaa agccgccgaa agttcctcga agctagcttc agacgtgtct agatacgtct 9420gctttttgtt gacttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaga 9480ggccaaaaag ctcgctttca gcacctgtcg tttcctttct tttcagaggg tattttaaat 9540aaaaacatta agttatgacg aagaagaacg gaaacgcctt aaaccggaaa attttcataa 9600atagcgaaaa cccgcgaggt cgccgccccg taacaaggcg gatcgccgga aaggacccgc 9660
aaatgataat aattatcaat tgcatactat cgacggcact gctgccagat aacaccaccg 9720gggaaacatt ccatcatgat ggccgtgcgg acataggaag ccagttcatc catcgctttc 9780ttgtctgctg ccatttgctt tgtgacatcc agcgccgcac attcagcagc gtttttcagc 9840gcgttttcga tcaacgtttc aatgttggta tcaacaccag gtttaacttt gaacttatcg 9900gcactgacgg ttaccttgtt ctgcgctggc tcatcacgca ggataccaag gctgatgttg 9960tagatattgg tcaccggctg agggttttcg attgccgctg cgtggatagc accatttgcg 10020atcaggcngt ccttgatgaa tgacactcca ttgcgaataa gttcgaagga gacggtgtca 10080cgaatgcgct ggtccagctc ggtcgattgc cttttgtgca gcagaggtat caatctcaac 10140gccaaggctc atcgaagcgc aatattgctg ctcaccaaaa cgcgtattga ccaggtgttc 10200aacggcaaat ttctgccctt ctgatgtcag aaaggcaaag tgattttctt tctggtattc 10260agttgctgtg tgtcggtttc agcaaaacca agctcgcgca attcggctgt gcagatttag 10320aaggcagatc accagacagc aacggccaac ggaaaacagc gcatacagaa catccgtcgc 10380cgcgccgaca acgtgataat ttttatgacc catgatttat ttccttttag acgtgagcct 10440gtcgcacagc aaagccgccg aaagttcctc gaccgatgcc cttgagagcc ttcaacccag 10500tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 10560ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 10620gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 10680ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 10740ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 10800gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 10860tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 10920tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg 10980
ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 11040tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag 11100ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg 11160gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc 11220cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tgcagatccg gctgtggaat 11280gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 11340atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 11400agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 11460atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 11520tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 11580ggcttttttg gaggcctagg cttttgcaaa a 11611<210>5<211>21<212>DNA<213>人工序列<220>
<223>引物<400>5cgatgtagga gggcgtggat a 21<210>6<211>21<212>DNA<213>人工序列<220>
<223>引物
<400>6gcttctgcgg gcgatttgtg t 21<210>7<211>20<212>DNA<213>人工序列<220>
<223>引物<400>7tgagaatatc accggaattg 20<210>8<211>21<212>DNA<213>人工序列<220>
<223>引物<400>8agctcgacat actgttcttc c 21<210>9<211>24<212>DNA<213>人工序列<220>
<223>引物<400>9gtgaatggaa atcccatcgc tgtc24<210>10<211>24<212>DNA<213>人工序列
<220>
<223>引物<400>10agtgggtact ctaaaggcca tacc24<210>11<211>1771<212>DNA<213>雨生紅球藻(Haematococcus pluvialis)<220>
<221>CDS<222>(166)..(1155)<400>11ggcacgagct tgcacgcaag tcagcgcgcg caagtcaaca cctgccggtc cacagcctca 60aataataaag agctcaagcg tttgtgcgcc tcgacgtggc cagtctgcac tgccttgaac120ccgcgagtct cccgccgcac tgactgccat agcacagcta gacga atg cag cta gca177Met Gln Leu Ala1gcg aca gta atg ttg gag cag ctt acc gga agc gct gag gca ctc aag 225Ala Thr Val Met Leu Glu Gln Leu Thr Gly Ser Ala Glu Ala Leu Lys5 10 15 20gag aag gag aag gag gtt gca ggc agc tct gac gtg ttg cgt aca tgg 273Glu Lys Glu Lys Glu Val Ala Gly Ser Ser Asp Val Leu Arg Thr Trp25 30 35gcg acc cag tac tcg ctt ccg tca gaa gag tca gac gcg gcc cgc ccg 321Ala Thr Gln Tyr Ser Leu Pro Ser Glu Glu Ser Asp Ala Ala Arg Pro40 45 50gga ctg aag aat gcc tac aag cca cca cct tcc gac aca aag ggc atc 369Gly Leu Lys Asn Ala Tyr Lys Pro Pro Pro Ser Asp Thr Lys Gly Ile55 60 65aca atg gcg cta cgt gtc atc ggc tcc tgg gcc gca gtg ttc ctc cac 417
Thr Met Ala Leu Arg Val Ile Gly Ser Trp Ala Ala Val Phe Leu His70 75 80gcc att ttt caa atc aag ctt ccg acc tcc ttg gac cag ctg cac tgg 465Ala Ile Phe Gln Ile Lys Leu Pro Thr Ser Leu Asp Gln Leu His Trp85 90 95 100ctg ccc gtg tca gat gcc aca gct cag ctg gtt agc ggc acg agc agc 513Leu Pro Val Ser Asp Ala Thr Ala Gln Leu Val Ser Gly Thr Ser Ser105 110 115ctg ctc gac atc gtc gta gta ttc ttt gtc ctg gag ttc ctg tac aca 561Leu Leu Asp Ile Val Val Val Phe Phe Val Leu Glu Phe Leu Tyr Thr120 125 130ggc ctt ttt atc acc acg cat gat gct atg cat ggc acc atc gcc atg 609Gly Leu Phe Ile Thr Thr His Asp Ala Met His Gly Thr Ile Ala Met135 140 145aga aac agg cag ctt aat gac ttc ttg ggc aga gta tgc atc tcc ttg 657Arg Asn Arg Gln Leu Asn Asp Phe Leu Gly Arg Val Cys Ile Ser Leu150 155 160tac gcc tgg ttt gat tac aac atg ctg cac cgc aag cat tgg gag cac 705Tyr Ala Trp Phe Asp Tyr Asn Met Leu His Arg Lys His Trp Glu His165 170 175 180cac aac cac act ggc gag gtg ggc aag gac cct gac ttc cac agg gga 753His Asn His Thr Gly Glu Val Gly Lys Asp Pro Asp Phe His Arg Gly185 190 195aac cct ggc att gtg ccc tgg ttt gcc agc ttc atg tcc agc tac atg 801Asn Pro Gly Ile Val Pro Trp Phe Ala Ser Phe Met Ser Ser Tyr Met200 205 210tcg atg tgg cag ttt gcg cgc ctc gca tgg tgg acg gtg gtc atg cag 849Ser Met Trp Gln Phe Ala Arg Leu Ala Trp Trp Thr Val Val Met Gln215 220 225ctg ctg ggt gcg cca atg gcg aac ctg ctg gtg ttc atg gcg gcc gcg 897Leu Leu Gly Ala Pro Met Ala Asn Leu Leu Val Phe Met Ala Ala Ala230 235 240ccc atc ctg tcc gcc ttc cgc ttg ttc tac ttt ggc acg tac atg ccc 945
Pro Ile Leu Ser Ala Phe Arg Leu Phe Tyr Phe Gly Thr Tyr Met Pro245 250 255 260cac aag cct gag cct ggc gcc gcg tca ggc tct tca cca gcc gtc atg 993His Lys Pro Glu Pro Gly Ala Ala Ser Gly Ser Ser Pro Ala Val Met265 270 275aac tgg tgg aag tcg cgc act agc cag gcg tcc gac ctg gtc agc ttt 1041Asn Trp Trp Lys Ser Arg Thr Ser Gln Ala Ser Asp Leu Val Ser Phe280 285 290ctg acc tgc tac cac ttc gac ctg cac tgg gag cac cac cgc tgg ccc 1089Leu Thr Cys Tyr His Phe Asp Leu His Trp Glu His His Arg Trp Pro295 300 305ttc gcc ccc tgg tgg gag ctg ccc aac tgc cgc cgc ctg tct ggc cga 1137Phe Ala Pro Trp Trp Glu Leu Pro Asn Cys Arg Arg Leu Ser Gly Arg310 315 320ggt ctg gtt cct gcc tag ctggacacac tgcagtgggc cctgctgcca1185Gly Leu Val Pro Ala325gctgggcatg caggttgtgg caggactggg tgaggtgaaa agctgcaggc gctgctgccg 1245gacacgctgc atgggctacc ctgtgtagct gccgccacta ggggaggggg tttgtagctg 1305tcgagcttgc cccatggatg aagctgtgta gtggtgcagg gagtacaccc acaggccaac 1365acccttgcag gagatgtctt gcgtcgggag gagtgttggg cagtgtagat gctatgattg 1425tatcttaatg ctgaagcctt taggggagcg acacttagtg ctgggcaggc aacgccctgc 1485aaggtgcagg cacaagctag gctggacgag gactcggtgg caggcaggtg aagaggtgcg 1545ggagggtggt gccacaccca ctgggcaaga ccatgctgca atgctggcgg tgtggcagtg 1605agagctgcgt gattaactgg gctatggatt gtttgagcag tctcacttat tctttgatat 1665agatactggt caggcaggtc aggagagtga gtatgaacaa gttgagaggt ggtgcgctgc 1725ccctgcgctt atgaagctgt aacaataaag tggttcaaaa aaaaaa 1771
<210>12<211>329<212>PRT<213>雨生紅球藻<400>12Met Gln Leu Ala Ala Thr Val Met Leu Glu Gln Leu Thr Gly Ser Ala1 5 10 15Glu Ala Leu Lys Glu Lys Glu Lys Glu Val Ala Gly Ser Ser Asp Val20 25 30Leu Arg Thr Trp Ala Thr Gln Tyr Ser Leu Pro Ser Glu Glu Ser Asp35 40 45Ala Ala Arg Pro Gly Leu Lys Asn Ala Tyr Lys Pro Pro Pro Ser Asp50 55 60Thr Lys Gly Ile Thr Met Ala Leu Arg Val Ile Gly Ser Trp Ala Ala65 70 75 80Val Phe Leu His Ala Ile Phe Gln lle Lys Leu Pro Thr Ser Leu Asp85 90 95Gln Leu His Trp Leu Pro Val Ser Asp Ala Thr Ala Gln Leu Val Ser100 105 110Gly Thr Ser Ser Leu Leu Asp Ile Val Val Val Phe Phe Val Leu Glu115 120 125Phe Leu Tyr Thr Gly Leu Phe Ile Thr Thr His Asp Ala Met His Gly130 135 140Thr Ile Ala Met Arg Asn Arg Gln Leu Asn Asp Phe Leu Gly Arg Val
145 150 155 160Cys Ile Ser Leu Tyr Ala Trp Phe Asp Tyr Asn Met Leu His Arg Lys165 170 175His Trp Glu His His Asn His Thr Gly Glu Val Gly Lys Asp Pro Asp180 185 190Phe His Arg Gly Asn Pro Gly Ile Val Pro Trp Phe Ala Ser Phe Met195 200 205Ser Ser Tyr Met Ser Met Trp Gln Phe Ala Arg Leu Ala Trp Trp Thr210 215 220Val Val Met Gln Leu Leu Gly Ala Pro Met Ala Asn Leu Leu Val Phe225 230 235 240Met Ala Ala Ala Pro Ile Leu Ser Ala Phe Arg Leu Phe Tyr Phe Gly245 250 255Thr Tyr Met Pro His Lys Pro Glu Pro Gly Ala Ala Ser Gly Ser Ser260 265 270Pro Ala Val Met Asn Trp Trp Lys Ser Arg Thr Ser Gln Ala Ser Asp275 280 285Leu Val Ser Phe Leu Thr Cys Tyr His Phe Asp Leu His Trp Glu His290 295 300His Arg Trp Pro Phe Ala Pro Trp Trp Glu Leu Pro Asn Cys Arg Arg305 310 315 320Leu Ser Gly Arg Gly Leu Val Pro Ala
325<210>13<211>1662<212>DNA<213>雨生紅球藻<220>
<221>CDS<222>(168)..(1130)<400>13cggggcaact caagaaattc aacagctgca agcgcgcccc agcctcacag cgccaagtga 60gctatcgacg tggttgtgag cgctcgacgt ggtccactga cgggcctgtg agcctctgcg120ctccgtcctc tgccaaatct cgcgtcgggg cctgcctaag tcgaaga atg cac gtc 176Met His Val1gca tcg gca cta atg gtc gag cag aaa ggc agt gag gca gct gct tcc 224Ala Ser Ala Leu Met Val Glu Gln Lys Gly Ser Glu Ala Ala Ala Ser5 10 15agc cca gac gtc ttg aga gcg tgg gcg aca cag tat cac atg cca tcc 272Ser Pro Asp Val Leu Arg Ala Trp Ala Thr Gln Tyr His Met Pro Ser20 25 30 35gag tcg tca gac gca gct cgt cct gcg cta aag cac gcc tac aaa cct 320Glu Ser Ser Asp Ala Ala Arg Pro Ala Leu Lys His Ala Tyr Lys Pro40 45 50cca gca tct gac gcc aag ggc atc acg atg gcg ctg acc atc att ggc 368Pro Ala Ser Asp Ala Lys Gly Ile Thr Met Ala Leu Thr Ile Ile Gly55 60 65acc tgg acc gca gtg ttt tta cac gca ata ttt caa atc agg cta ccg 416Thr Trp Thr Ala Val Phe Leu His Ala Ile Phe Gln Ile Arg Leu Pro70 75 80aca tcc atg gac cag ctt cac tgg ttg cct gtg tcc gaa gcc aca gcc 464Thr Ser Met Asp Gln Leu His Trp Leu Pro Val Ser Glu Ala Thr Ala
85 90 95cag ctt ttg ggc gga agc agc agc cta ctg cac atc gct gca gtc ttc 512Gln Leu Leu Gly Gly Ser Ser Ser Leu Leu His Ile Ala Ala Val Phe100 105 110 115att gta ctt gag ttc ctg tac act ggt cta ttc atc acc aca cat gac 560Ile Val Leu Glu Phe Leu Tyr Thr Gly Leu Phe Ile Thr Thr His Asp120 125 130gca atg cat ggc acc ata gct ttg agg cac agg cag ctc aat gat ctc 608Ala Met His Gly Thr Ile Ala Leu Arg His Arg Gln Leu Asn Asp Leu135 140 145ctt ggc aac atc tgc ata tca ctg tac gcc tgg ttt gac tac agc atg 656Leu Gly Asn Ile Cys Ile Ser Leu Tyr Ala Trp Phe Asp Tyr Ser Met150 155 160ctg cat cgc aag cac tgg gag cac cac aac cat act ggc gaa gtg ggg 704Leu His Arg Lys His Trp Glu His His Asn His Thr Gly Glu Val Gly165 170 175aaa gac cct gac ttc cac aag gga aat ccc ggc ctt gtc ccc tgg ttc 752Lys Asp Pro Asp Phe His Lys Gly Asn Pro Gly Leu Val Pro Trp Phe180 185 190 195gcc agc ttc atg tcc agc tac atg tcc ctg tgg cag ttt gcc cgg ctg 800Ala Ser Phe Met Ser Ser Tyr Met Ser Leu Trp Gln Phe Ala Arg Leu200 205 210gca tgg tgg gca gtg gtg atg caa atg ctg ggg gcg ccc atg gca aat 848Ala Trp Trp Ala Val Val Met Gln Met Leu Gly Ala Pro Met Ala Asn215 220 225ctc cta gtc ttc atg gct gca gcc cca atc ttg tca gca ttc cgc ctc 896Leu Leu Val Phe Met Ala Ala Ala Pro Ile Leu Ser Ala Phe Arg Leu230 235 240ttc tac ttc ggc act tac ctg cca cac aag cct gag cca ggc cct gca 944Phe Tyr Phe Gly Thr Tyr Leu Pro His Lys Pro Glu Pro Gly Pro Ala245 250 255gca ggc tct cag gtg atg gcc tgg ttc agg gcc aag aca agt gag gca 992Ala Gly Ser Gln Val Met Ala Trp Phe Arg Ala Lys Thr Ser Glu Ala
260 265 270 275tct gat gtg atg agt ttc ctg aca tgc tac cac ttt gac ctg cae tgg 1040Ser Asp Val Met Ser Phe Leu Thr Cys Tyr His Phe Asp Leu His Trp280 285 290gag cac cac agg tgg ccc ttt gcc ccc tgg tgg cag ctg ccc cac tgc 1088Glu His His Arg Trp Pro Phe Ala Pro Trp Trp Gln Leu Pro His Cys295 300 305cgc cgc ctg tcc ggg cgt ggc ctg gtg cct gcc ttg gca tga 1130Arg Arg Leu Ser Gly Arg Gly Leu Val Pro Ala Leu Ala310 315 320cctggtccct ccgctggtga cccagcgtct gcacaagagt gtcatgctac agggtgctgc 1190ggccagtggc agcgcagtgc actctcagcc tgtatggggc taccgctgtg ccactgagca 1250ctgggcatgc cactgagcac tgggcgtgct actgagcaat gggcgtgcta ctgagcaatg 1310ggcgtgctac tgacaatggg cgtgctactg gggtctggca gtggctagga tggagtttga 1370tgcattcagt agcggtggcc aacgtcatgt ggatggtgga agtgctgagg ggtttaggca 1430gccggcattt gagagggcta agttataaat cgcatgctgc tcatgcgcac atatctgcac 1490acagccaggg aaatcccttc gagagtgatt atgggacact tgtattggtt tcgtgctatt 1550gttttattca gcagcagtac ttagtgaggg tgagagcagg gtggtgagag tggagtgagt 1610gagtatgaac ctggtcagcg aggtgaacag cctgtaatga atgactctgt ct 1662<210>14<211>320<212>PRT<213>雨生紅球藻<400>14Met His Val Ala Ser Ala Leu Met Val Glu Gln Lys Gly Ser Glu Ala1 5 10 15
Ala Ala Ser Ser Pro Asp Val Leu Arg Ala Trp Ala Thr Gln Tyr His20 25 30Met Pro Ser Glu Ser Ser Asp Ala Ala Arg Pro Ala Leu Lys His Ala35 40 45Tyr Lys Pro Pro Ala Ser Asp Ala Lys Gly Ile Thr Met Ala Leu Thr50 55 60Ile Ile Gly Thr Trp Thr Ala Val Phe Leu His Ala Ile Phe Gln Ile65 70 75 80Arg Leu Pr0 Thr Ser Met Asp Gln Leu His Trp Leu Pro Val Ser Glu85 90 95Ala Thr Ala Gln Leu Leu Gly Gly Ser Ser Ser Leu Leu His Ile Ala100 105 110Ala Val Phe Ile Val Leu Glu Phe Leu Tyr Thr Gly Leu Phe Ile Thr115 120 125Thr His Asp Ala Met His Gly Thr Ile Ala Leu Arg His Arg Gln Leu130 135 140Asn Asp Leu Leu Gly Asn Ile Cys Ile Ser Leu Tyr Ala Trp Phe Asp145 150 155 160Tyr Ser Met Leu His Arg Lys His Trp Glu His His Asn His Thr Gly165 170 175Glu Val Gly Lys Asp Pro Asp Phe His Lys Gly Asn Pro Gly Leu Val180 185 190
Pro Trp Phe Ala Ser Phe Met Ser Ser Tyr Met Ser Leu Trp Gln Phe195 200 205Ala Arg Leu Ala Trp Trp Ala Val Val Met Gln Met Leu Gly Ala Pro210 215 220Met Ala Ash Leu Leu Val Phe Met Ala Ala Ala Pro Ile Leu Ser Ala225 230 235 240Phe Arg Leu Phe Tyr Phe Gly Thr Tyr Leu Pro His Lys Pro Glu Pro245 250 255Gly Pro Ala Ala Gly Ser Gln Val Met Ala Trp Phe Arg Ala Lys Thr260 265 270Ser Glu Ala Ser Asp Val Met Ser Phe Leu Thr Cys Tyr His Phe Asp275 280 285Leu His Trp Glu His His Arg Trp Pro Phe Ala Pro Trp Trp Gln Leu290 295 300Pro His Cys Arg Arg Leu Ser Gly Arg Gly Leu Val Pro Ala Leu Ala305 310 315 320<210>15<211>729<212>DNA<213>橙黃農(nóng)桿菌(Agrobacterium aurantiacum)<220>
<221>CDS<222>(1)..(729)<400>15atg agc gca cat gcc ctg ccc aag gca gat ctg acc gcc acc agc ctg 48
Met Ser Ala His Ala Leu Pro Lys Ala Asp Leu Thr Ala Thr Ser Leu1 5 10 15atc gtc tcg ggc ggc atc atc gcc gct tgg ctg gcc ctg cat gtg cat 96Ile Val Ser Gly Gly Ile Ile Ala Ala Trp Leu Ala Leu His Val His20 25 30gcg ctg tgg ttt ctg gac gca gcg gcg cat ccc atc ctg gcg atc gca 144Ala Leu Trp Phe Leu Asp Ala Ala Ala His Pro Ile Leu Ala Ile Ala35 40 45aat ttc ctg ggg ctg acc tgg ctg tcg gtc gga ttg ttc atc atc gcg 192Asn Phe Leu Gly Leu Thr Trp Leu Ser Val Gly Leu Phe Ile Ile Ala50 55 60cat gac gcg atg cac ggg tcg gtg gtg ccg ggg cgt ccg cgc gcc aat 240His Asp Ala Met His Gly Ser Val Val Pro Gly Arg Pro Arg Ala Asn65 70 75 80gcg gcg atg ggc cag ctt gtc ctg tgg ctg tat gcc gga ttt tcg tgg 288Ala Ala Met Gly Gln Leu Val Leu Trp Leu Tyr Ala Gly Phe Ser Trp85 90 95cgc aag atg atc gtc aag cac atg gcc cat cac cgc cat gcc gga acc 336Arg Lys Met Ile Val Lys His Met Ala His His Arg His Ala Gly Thr100 105 110gac gac gac ccc gat ttc gac cat ggc ggc ccg gtc cgc tgg tac gcc 384Asp Asp Asp Pro Asp Phe Asp His Gly Gly Pro Val Arg Trp Tyr Ala115 120 125cgc ttc atc ggc acc tat ttc ggc tgg cgc gag ggg ctg ctg ctg ccc 432Arg Phe Ile Gly Thr Tyr Phe Gly Trp Arg Glu Gly Leu Leu Leu Pro130 135 140gtc atc gtg acg gtc tat gcg ctg atc ctt ggg gat cgc tgg atg tac 480Val Ile Val Thr Val Tyr Ala Leu Ile Leu Gly Asp Arg Trp Met Tyr145 150 155 160gtg gtc ttc tgg ccg ctg ccg tcg atc ctg gcg tcg atc cag ctg ttc 528Val Val Phe Trp Pro Leu Pro Ser Ile Leu Ala Ser Ile Gln Leu Phe165 170 175gtg ttc ggc acc tgg ctg ccg cac cgc ccc ggc cac gac gcg ttc ccg 576
Val Phe Gly Thr Trp Leu Pro His Arg Pro Gly His Asp Ala Phe Pro180 185 190gac cgc cac aat gcg cgg tcg tcg cgg atc agc gac ccc gtg tcg ctg 624Asp Arg His Asn Ala Arg Ser Ser Arg Ile Ser Asp Pro Val Ser Leu195 200 205ctg acc tgc ttt cac ttt ggc ggt tat cat cac gaa cac cac ctg cac 672Leu Thr Cys Phe His Phe Gly Gly Tyr His His Glu His His Leu His210 215 220ccg acg gtg ccg tgg tgg cgc ctg ccc agc acc cgc acc aag ggg gac 720Pro Thr Val Pro Trp Trp Arg Leu Pro Ser Thr Arg Thr Lys Gly Asp225 230 235 240acc gca tga 729Thr Ala<210>16<211>242<212>PRT<213>橙黃農(nóng)桿菌<400>16Met Ser Ala His Ala Leu Pro Lys Ala Asp Leu Thr Ala Thr Ser Leu1 5 10 15Ile Val Ser Gly Gly Ile Ile Ala Ala Trp Leu Ala Leu His Val His20 25 30Ala Leu Trp Phe Leu Asp Ala Ala Ala His Pro Ile Leu Ala Ile Ala35 40 45Asn Phe Leu Gly Leu Thr Trp Leu Ser Val Gly Leu Phe Ile Ile Ala50 55 60His Asp Ala Met His Gly Ser Val Val Pro Gly Arg Pro Arg Ala Asn
65 70 75 80Ala Ala Met Gly Gln Leu Val Leu Trp Leu Tyr Ala Gly Phe Ser Trp85 90 95Arg Lys Met Ile Val Lys His Met Ala His Hi s Arg His Ala Gly Thr100 105 110Asp Asp Asp Pro Asp Phe Asp His Gly Gly Pro Val Arg Trp Tyr Ala115 120 125Arg Phe Ile Gly Thr Tyr Phe Gly Trp Arg Glu Gly Leu Leu Leu Pro130 135 140Val Ile Val Thr Val Tyr Ala Leu Ile Leu Gly Asp Arg Trp Met Tyr145 150 155 160Val Val Phe Trp Pro Leu Pro Ser Ile Leu Ala Ser Ile Gln Leu Phe165 170 175Val Phe Gly Thr Trp Leu Pro His Arg Pro Gly His Asp Ala Phe Pro180 185 190Asp Arg His Asn Ala Arg Ser Ser Arg Ile Ser Asp Pro Val Ser Leu195 200 205Leu Thr Cys Phe His Phe Gly Gly Tyr His His Glu Hi s His Leu His210 215 220Pro Thr Val Pro Trp Trp Arg Leu Pro Ser Thr Arg Thr Lys Gly Asp225 230 235 240Thr Ala
<210>17<211>1631<212>DNA<213>產(chǎn)堿菌(Alcaligenes sp.)<220>
<221>CDS<222>(99)..(827)<400>17ctgcaggccg ggcccggtgg ccaatggtcg caaccggcag gactggaaca ggacggcggg 60ccggtctagg ctgtcgccct acgcagcagg agtttcgg atg tcc gga cgg aag cct116Met Ser Gly Arg Lys Pro1 5ggc aca act ggc gac acg atc gtc aat ctc ggt ctg acc gcc gcg atc 164Gly Thr Thr Gly Asp Thr Ile Val Asn Leu Gly Leu Thr Ala Ala Ile10 15 20ctg ctg tgc tgg ctg gtc ctg cac gcc ttt acg cta tgg ttg cta gat 212Leu Leu Cys Trp Leu Val Leu His Ala Phe Thr Leu Trp Leu Leu Asp25 30 35gcg gcc gcg cat ccg ctg ctt gcc gtg ctg tgc ctg gct ggg ctg acc 260Ala Ala Ala His Pro Leu Leu Ala Val Leu Cys Leu Ala Gly Leu Thr40 45 50tgg ctg tcg gtc ggg ctg ttc atc atc gcg cat gac gca atg cac ggg 308Trp Leu Ser Val Gly Leu Phe Ile Ile Ala His Asp Ala Met His Gly55 60 65 70tcc gtg gtg ccg ggg cgg ccg cgc gcc aat gcg gcg atc ggg caa ctg 356Ser Val Val Pro Gly Arg Pro Arg Ala Asn Ala Ala Ile Gly Gln Leu75 80 85gcg ctg tgg ctc tat gcg ggg ttc tcg tgg ccc aag ctg atc gcc aag 404Ala Leu Trp Leu Tyr Ala Gly Phe Ser Trp Pro Lys Leu Ile Ala Lys90 95 100
cac atg acg cat cac cgg cac gcc ggc acc gac aac gat ccc gat ttc 452His Met Thr His His Arg His Ala Gly Thr Asp Asn Asp Pro Asp Phe105 110 115ggt cac gga ggg ccc gtg cgc tgg tac ggc agc ttc gtc tcc acc tat 500Gly His Gly Gly Pro Val Arg Trp Tyr Gly Ser Phe Val Ser Thr Tyr120 125 130ttc ggc tgg cga gag gga ctg ctg cta ccg gtg atc gtc acc acc tat 548Phe Gly Trp Arg Glu Gly Leu Leu Leu Pro Val Ile Val Thr Thr Tyr135 140 145 150gcg ctg atc ctg ggc gat cgc tgg atg tat gtc atc ttc tgg ccg gtc 596Ala Leu Ile Leu Gly Asp Arg Trp Met Tyr Val Ile Phe Trp Pro Val155 160 165ccg gcc gtt ctg gcg tcg atc cag att ttc gtc ttc gga act tgg ctg 644Pro Ala Val Leu Ala Ser Ile Gln Ile Phe Val Phe Gly Thr Trp Leu170 175 180ccc cac cgc ccg gga cat gac gat ttt ccc gac cgg cac aac gcg agg 692Pro His Arg Pro Gly His Asp Asp Phe Pro Asp Arg His Asn Ala Arg185 190 195tcg acc ggc atc ggc gac ccg ttg tca cta ctg acc tgc ttc cat ttc 740Ser Thr Gly Ile Gly Asp Pro Leu Ser Leu Leu Thr Cys Phe His Phe200 205 210ggc ggc tat cac cac gaa cat cac ctg cat ccg cat gtg ccg tgg tgg 788Gly Gly Tyr His His Glu His His Leu His Pro His Val Pro Trp Trp215 220 225 230cgc ctg cct cgt aca cgc aag acc gga ggc cgc gca tga cgcaattcct 837Arg Leu Pro Arg Thr Arg Lys Thr Gly Gly Arg Ala235 240cattgtcgtg gcgacagtcc tcgtgatgga gctgaccgcc tattccgtcc accgctggat897tatgcacggc cccctaggct ggggctggca caagtcccat cacgaagagc acgaccacgc957gttggagaag aacgacctct acggcgtcgt cttcgcggtg ctggcgacga tcctcttcac 1017cgtgggcgcc tattggtggc cggtgctgtg gtggatcgcc ctgggcatga cggtctatgg 1077
gttgatctat ttcatcctgc acgacgggct tgtgcatcaa cgctggccgt ttcggtatat 1137tccgcggcgg ggctatttcc gcaggctcta ccaagctcat cgcctgcacc acgcggtcga 1197ggggcgggac cactgcgtca gcttcggctt catctatgcc ccacccgtgg acaagctgaa 1257gcaggatctg aagcggtcgg gtgtcctgcg cccccaggac gagcgtccgt cgtgatctct 1317gatcccggcg tggccgcatg aaatccgacg tgctgctggc aggggccggc cttgccaacg 1377gactgatcgc gctggcgatc cgcaaggcgc ggcccgacct tcgcgtgctg ctgctggacc 1437gtgcggcggg cgcctcggac gggcatactt ggtcctgcca cgacaccgat ttggcgccgc 1497actggctgga ccgcctgaag ccgatcaggc gtggcgactg gcccgatcag gaggtgcggt 1557tcccagacca ttcgcgaagg ctccgggccg gatatggctc gatcgacggg cgggggctga 1617tgcgtgcggt gacc 1631<210>18<211>242<212>PRT<213>產(chǎn)堿菌<400>18Met Ser Gly Arg Lys Pro Gly Thr Thr Gly Asp Thr Ile Val Asn Leu1 5 10 15Gly Leu Thr Ala Ala Ile Leu Leu Cys Trp Leu Val Leu His Ala Phe20 25 30Thr Leu Trp Leu Leu Asp Ala Ala Ala His Pro Leu Leu Ala Val Leu35 40 45Cys Leu Ala Gly Leu Thr Trp Leu Ser Val Gly Leu Phe Ile Ile Ala50 55 60
His Asp Ala Met His Gly Ser Val Val Pro Gly Arg Pro Arg Ala Asn65 70 75 80Ala Ala Ile Gly Gln Leu Ala Leu Trp Leu Tyr Ala Gly Phe Ser Trp85 90 95Pro Lys Leu Ile Ala Lys His Met Thr His His Arg His Ala Gly Thr100 105 110Asp Asn Asp Pro Asp Phe Gly His Gly Gly Pro Val Arg Trp Tyr Gly115 120 125Ser Phe Val Ser Thr Tyr Phe Gly Trp Arg Glu Gly Leu Leu Leu Pro130 135 140Val Ile Val Thr Thr Tyr Ala Leu Ile Leu Gly Asp Arg Trp Met Tyr145 150 155 160Val Ile Phe Trp Pro Val Pro Ala Val Leu Ala Ser Ile Gln Ile Phe165 170 175Val Phe Gly Thr Trp Leu Pro His Arg Pro Gly His Asp Asp Phe Pro180 185 190Asp Arg His Asn Ala Arg Ser Thr Gly Ile Gly Asp Pro Leu Ser Leu195 200 205Leu Thr Cys Phe His Phe Gly Gly Tyr His His Glu His His Leu His210 215 220Pro His Val Pro Trp Trp Arg Leu Pro Arg Thr Arg Lys Thr Gly Gly225 230 235 240
Arg Ala<210>19<211>729<212>DNA<213>Paracoccus marcusii<220>
<221>CDS<222>(1)..(729)<400>19atg agc gca cat gcc ctg ccc aag gca gat ctg acc gcc aca agc ctg 48Met Ser Ala His Ala Leu Pro Lys Ala Asp Leu Thr Ala Thr Ser Leu1 5 10 15atc gtc tcg ggc ggc atc atc gcc gca tgg ctg gcc ctg cat gtg cat 96Ile Val Ser Gly Gly Ile Ile Ala Ala Trp Leu Ala Leu His Val His20 25 30gcg ctg tgg ttt ctg gac gcg gcg gcc cat ccc atc ctg gcg gtc gcg 144Ala Leu Trp Phe Leu Asp Ala Ala Ala His Pro Ile Leu Ala Val Ala35 40 45aat ttc ctg ggg ctg acc tgg ctg tcg gtc gga ttg ttc atc atc gcg 192Asn Phe Leu Gly Leu Thr Trp Leu Ser Val Gly Leu Phe Ile Ile Ala50 55 60cat gac gcg atg cac ggg tcg gtc gtg ccg ggg cgt ccg cgc gcc aat 240His Asp Ala Met His Gly Ser Val Val Pro Gly Arg Pro Arg Ala Asn65 70 75 80gcg gcg atg ggc cag ctt gtc ctg tgg ctg tat gcc gga ttt tcg tgg 288Ala Ala Met Gly Gln Leu Val Leu Trp Leu Tyr Ala Gly Phe Ser Trp85 90 95cgc aag atg atc gtc aag cac atg gcc cat cac cgc cat gcc gga acc 336Arg Lys Met Ile Val Lys His Met Ala His His Arg His Ala Gly Thr100 105 110gac gac gac cca gat ttc gac cat ggc ggc ccg gtc cgc tgg tac gcc 384
Asp Asp Asp Pro Asp Phe Asp His Gly Gly Pro Val Arg Trp Tyr Ala115 120 125cgc ttc atc ggc acc tat ttc ggc tgg cgc gag ggg ctg ctg ctg ccc 432Arg Phe Ile Gly Thr Tyr Phe Gly Trp Arg Glu Gly Leu Leu Leu Pro130 135 140gtc atc gtg acg gtc tat gcg ctg atc ctg ggg gat cgc tgg atg tac 480Val Ile Val Thr Val Tyr Ala Leu Ile Leu Gly Asp Arg Trp Met Tyr145 150 155 160gtg gtc ttc tgg ccg ttg ccg tcg atc ctg gcg tcg atc cag ctg ttc 528Val Val Phe Trp Pro Leu Pro Ser Ile Leu Ala Ser Ile Gln Leu Phe165 170 175gtg ttc ggc act tgg ctg ccg cac cgc ccc ggc cac gac gcg ttc ccg 576Val Phe Gly Thr Trp Leu Pro His Arg Pro Gly His Asp Ala Phe Pro180 185 190gac cgc cat aat gcg cgg tcg tcg cgg atc agc gac cct gtg tcg ctg 624Asp Arg His Asn Ala Arg Ser Ser Arg Ile Ser Asp Pro Val Ser Leu195 200 205ctg acc tgc ttt cat ttt ggc ggt tat cat cac gaa cac cac ctg cac 672Leu Thr Cys Phe His Phe Gly Gly Tyr His His Glu His His Leu His210 215 220ccg acg gtg ccg tgg tgg cgc ctg ccc agc acc cgc acc aag ggg gac 720Pro Thr Val Pro Trp Trp Arg Leu Pro Ser Thr Arg Thr Lys Gly Asp225 230 235 240acc gca tga 729Thr Ala<210>20<211>242<212>PRT<213>Paracoccus marcusii<400>20Met Ser Ala His Ala Leu Pro Lys Ala Asp Leu Thr Ala Thr Ser Leu
1 5 10 15Ile Val Ser Gly Gly Ile Ile Ala Ala Trp Leu Ala Leu His Val His20 25 30Ala Leu Trp Phe Leu Asp Ala Ala Ala His Pro Ile Leu Ala Val Ala35 40 45Asn Phe Leu Gly Leu Thr Trp Leu Ser Val Gly Leu Phe Ile Ile Ala50 55 60His Asp Ala Met His Gly Ser Val Val Pro Gly Arg Pro Arg Ala Asn65 70 75 80Ala Ala Met Gly Gln Leu Val Leu Trp Leu Tyr Ala Gly Phe Ser Trp85 90 95Arg Lys Met Ile Val Lys His Met Ala His His Arg His Ala Gly Thr100 105 110Asp Asp Asp Pro Asp Phe Asp His Gly Gly Pro Val Arg Trp Tyr Ala115 120 125Arg Phe Ile Gly Thr Tyr Phe Gly Trp Arg Glu Gly Leu Leu Leu Pro130 135 140Val Ile Val Thr Val Tyr Ala Leu Ile Leu Gly Asp Arg Trp Met Tyr145 150 155 160Val Val Phe Trp Pro Leu Pro SerIle Leu Ala SerIle Gln Leu Phe165170175Val Phe Gly Thr Trp Leu Pro His Arg Pro Gly His Asp Ala Phe Pro
180 185 190Asp Arg His Asn Ala Arg Ser Ser Arg Ile Ser Asp Pro Val Ser Leu195 200 205Leu Thr Cys Phe His Phe Gly Gly Tyr His His Glu His His Leu His210 215 220Pro Thr Val Pro Trp Trp Arg Leu Pro Ser Thr Arg Thr Lys Gly Asp225 230 235 240Thr Ala<210>21<211>1629<212>DNA<213>集胞藻(Synechocystis sp.)<220>
<221>CDS<222>(1)..(1629)<400>21atg atc acc acc gat gtt gtc att att ggg gcg ggg cac aat ggc tta 48Met Ile Thr Thr Asp Val Val Ile Ile Gly Ala Gly His Asn Gly Leu1 5 10 15gtc tgt gca gcc tat ttg ctc caa cgg ggc ttg ggg gtg acg tta cta 96Val Cys Ala Ala Tyr Leu Leu Gln Arg Gly Leu Gly Val Thr Leu Leu20 25 30gaa aag cgg gaa gta cca ggg ggg gcg gcc acc aca gaa gct ctc atg 144Glu Lys Arg Glu Val Pro Gly Gly Ala Ala Thr Thr Glu Ala Leu Met35 40 45ccg gag cta tcc ccc cag ttt cgc ttt aac cgc tgt gcc att gac cac 192Pro Glu Leu Ser Pro Gln Phe Arg Phe Asn Arg Cys Ala Ile Asp His
50 55 60gaa ttt atc ttt ctg ggg ccg gtg ttg cag gag cta aat tta gcc cag 240Glu Phe Ile Phe Leu Glv Pro Val Leu Gln Glu Leu Asn Leu Ala Gln65 70 75 80tat ggt ttg gaa tat tta ttt tgt gac ccc agt gtt ttt tgt ccg ggg 288Tyr Gly Leu Glu Tyr Leu Phe Cys Asp Pro Ser Val Phe Cys Pro Gly85 90 95ctg gat ggc caa gct ttt atg agc tac cgt tcc cta gaa aaa acc tgt 336Leu Asp Gly Gln Ala Phe Met Ser Tyr Arg Ser Leu Glu Lys Thr Cys100 105 110gcc cac att gcc acc tat agc ccc cga gat gcg gaa aaa tat cgg caa 384Ala His Ile Ala Thr Tyr Ser Pro Arg Asp Ala Glu Lys Tyr Arg Gln115 120 125ttt gtc aat tat tgg acg gat ttg ctc aac gct gtc cag cct gct ttt 432Phe Val Asn Tyr Trp Thr Asp Leu Leu Asn Ala Val Gln Pro Ala Phe130 135 140aat gct ccg ccc cag gct tta cta gat tta gcc ctg aac tat ggt tgg 480Asn Ala Pro Pro Gln Ala Leu Leu Asp Leu Ala Leu Asn Tyr Gly Trp145 150 155 160gaa aac tta aaa tcc gtg ctg gcg atc gcc ggg tcg aaa acc aag gcg 528Glu Asn Leu Lys Ser Val Leu Ala Ile Ala Gly Ser Lys Thr Lys Ala165 170 175ttg gat ttt atc cgc act atg atc ggc tcc ccg gaa gat gtg ctc aat 576Leu Asp Phe Ile Arg Thr Met Ile Gly Ser Pro Glu Asp Val Leu Asn180 185 190gaa tgg ttc gac agc gaa cgg gtt aaa gct cct tta gct aga cta tgt 624Glu Trp Phe Asp Ser Glu Arg Val Lys Ala Pro Leu Ala Arg Leu Cys195 200 205tcg gaa att ggc gct ccc cca tcc caa aag ggt agt agc tcc ggc atg 672Ser Glu Ile Gly Ala Pro Pro Ser Gln Lys Gly Ser Ser Ser Gly Met210 215 220atg atg gtg gcc atg cgg cat ttg gag gga att gcc aga cca aaa gga 720Met Met Val Ala Met Arg His Leu Glu Gly Ile Ala Arg Pro Lys Gly
225 230 235 240ggc act gga gcc ctc aca gaa gcc ttg gtg aag tta gtg caa gcc caa 768Gly Thr Gly Ala Leu Thr Glu Ala Leu Val Lys Leu Val Gln Ala Gln245 250 255ggg gga aaa atc ctc act gac caa acc gtc aaa cgg gta ttg gtg gaa 816Gly Gly Lys Ile Leu Thr Asp Gln Thr Val Lys Arg Val Leu Val Glu260 265 270aac aac cag gcg atc ggg gtg gag gta gct aac gga gaa cag tac cgg 864Asn Asn Gln Ala Ile Gly Val Glu Val Ala Asn Gly Glu Gln Tyr Arg275 280 285gcc aaa aaa ggc gtg att tct aac atc gat gcc cgc cgt tta ttt ttg 912Ala Lys Lys Gly Val Ile Ser Asn Ile Asp Ala Arg Arg Leu Phe Leu290 295 300caa ttg gtg gaa ccg ggg gcc cta gcc aag gtg aat caa aac cta ggg 960Gln Leu Val Glu Pro Gly Ala Leu Ala Lys Val Asn Gln Asn Leu Gly305 310 315 320gaa cga ctg gaa cgg cgc act gtg aac aat aac gaa gcc att tta aaa 1008Glu Arg Leu Glu Arg Arg Thr Val Asn Asn Asn Glu Ala Ile Leu Lys325 330 335atc gat tgt gcc ctc tcc ggt tta ccc cac ttc act gcc atg gcc ggg 1056Ile Asp Cys Ala Leu Ser Gly Leu Pro His Phe Thr Ala Met Ala Gly340 345 350ccg gag gat cta acg gga act att ttg att gcc gac tcg gta cgc cat 1104Pro Glu Asp Leu Thr Gly Thr Ile Leu Ile Ala Asp Ser Val Arg His355 360 365gtc gag gaa gcc cac gcc ctc att gcc ttg ggg caa att ccc gat gct 1152Val Glu Glu Ala His Ala Leu Ile Ala Leu Gly Gln Ile Pro Asp Ala370 375 380aat ccg tct tta tat ttg gat att ccc act gta ttg gac ccc acc atg 1200Asn Pro Ser Leu Tyr Leu Asp Ile Pro Thr Val Leu Asp Pro Thr Met385 390 395 400gcc ccc cct ggg cag cac acc ctc tgg atc gaa ttt ttt gcc ccc tac 1248Ala Pro Pro Gly Gln His Thr Leu Trp Ile Glu Phe Phe Ala Pro Tyr
405 410 415cgc atc gcc ggg ttg gaa ggg aca ggg tta atg ggc aca ggt tgg acc 1296Arg Ile Ala Gly Leu Glu Gly Thr Gly Leu Met Gly Thr Gly Trp Thr420 425 430gat gag tta aag gaa aaa gtg gcg gat cgg gtg att gat aaa tta acg 1344Asp Glu Leu Lys Glu Lys Val Ala Asp Arg Val Ile Asp Lys Leu Thr435 440 445gac tat gcc cct aac cta aaa tct ctg atc att ggt cgc cga gtg gaa 1392Asp Tyr Ala Pro Asn Leu Lys Ser Leu Ile Ile Gly Arg Arg Val Glu450 455 460agt ccc gcc gaa ctg gcc caa cgg ctg gga agt tac aac ggc aat gtc 1440Ser Pro Ala Glu Leu Ala Gln Arg Leu Gly Ser Tyr Asn Gly Asn Val465 470 475 480tat cat ctg gat atg agt ttg gac caa atg atg ttc ctc cgg cct cta 1488Tyr His Leu Asp Met Ser Leu Asp Gln Met Met Phe Leu Arg Pro Leu485 490 495ccg gaa att gcc aac tac caa acc ccc atc aaa aat ctt tac tta aca 1536Pro Glu Ile Ala Asn Tyr Gln Thr Pro Ile Lys Asn Leu Tyr Leu Thr500 505 510ggg gcg ggt acc cat ccc ggt ggc tcc ata tca ggt atg ccc ggt aga 1584Gly Ala Gly Thr His Pro Gly Gly Ser Ile Ser Gly Met Pro Gly Arg515 520 525aat tgc gct cgg gtc ttt tta aaa caa caa cgt cgt ttt tgg taa 1629Asn Cys Ala Arg Val Phe Leu Lys Gln Gln Arg Arg Phe Trp530 535 540<210>22<211>542<212>PRT<213>集胞藻<400>22Met Ile Thr Thr Asp Val Val Ile Ile Gly Ala Gly His Asn Gly Leu1 5 10 15
Val Cys Ala Ala Tyr Leu Leu Gln Arg Gly Leu Gly Val Thr Leu Leu20 25 30Glu Lys Arg Glu Val Pro Gly Gly Ala Ala Thr Thr Glu Ala Leu Met35 40 45Pro Glu Leu Ser Pro Gln Phe Arg Phe Asn Arg Cys Ala Ile Asp His50 55 60Glu Phe Ile Phe Leu Gly Pro Val Leu Gln Glu Leu Asn Leu Ala Gln65 70 75 80Tyr Gly Leu Glu Tyr Leu Phe Cys Asp Pro Ser Val Phe Cys Pro Gly85 90 95Leu Asp Gly Gln Ala Phe Met Ser Tyr Arg Ser Leu Glu Lys Thr Cys100 105 110Ala His Ile Ala Thr Tyr Ser Pro Arg Asp Ala Glu Lys Tyr Arg Gln115 120 125Phe Val Asn Tyr Trp Thr Asp Leu Leu Asn Ala Val Gln Pro Ala Phe130 135 140Asn Ala Pro Pro Gln Ala Leu Leu Asp Leu Ala Leu Asn Tyr Gly Trp145 150 155 160Glu Asn Leu Lys Ser Val Leu Ala Ile Ala Gly Ser Lys Thr Lys Ala165 170 175Leu Asp Phe Ile Arg Thr Met Ile Gly Ser Pro Glu Asp Val Leu Asn180 185 190
Glu Trp Phe Asp Ser Glu Arg Val Lys Ala Pro Leu Ala Arg Leu Cys195 200 205Ser Glu Ile Gly Ala Pro Pro Ser Gln Lys Gly Ser Ser Ser Gly Met210 215 220Met Met Val Ala Met Arg His Leu Glu Gly Ile Ala Arg Pro Lys Gly225 230 235240Gly Thr Gly Ala Leu Thr Glu Ala Leu Val Lys Leu Val Gln Ala Gln245 250 255Gly Gly Lys Ile Leu Thr Asp Gln Thr Val Lys Arg Val Leu Val Glu260 265 270Asn Asn Gln Ala Ile Gly Val Glu Val Ala Asn Gly Glu Gln Tyr Arg275 280 285Ala Lys Lys Gly Val Ile Ser Asn Ile Asp Ala Arg Arg Leu Phe Leu290 295 300Gln Leu Val Glu Pro Gly Ala Leu Ala Lys Val Asn Gln Asn Leu Gly305 310 315 320Glu Arg Leu Glu Arg Arg Thr Val Asn Asn Asn Glu Ala Ile Leu Lys325 330 335Ile Asp Cys Ala Leu Ser Gly Leu Pro His Phe Thr Ala Met Ala Gly340 345 350Pro Glu Asp Leu Thr Gly Thr Ile Leu Ile Ala Asp Ser Val Arg His355 360 365
Val Glu Glu Ala His Ala Leu Ile Ala Leu Gly Gln Ile Pro Asp Ala370 375 380Asn Pro Ser Leu Tyr Leu Asp Ile Pro Thr Val Leu Asp Pro Thr Met385 390 395 400Ala Pro Pro Gly Gln His Thr Leu Trp Ile Glu Phe Phe Ala Pro Tyr405 410 415Arg Ile Ala Gly Leu Glu Gly Thr Gly Leu Met Gly Thr Gly Trp Thr420 425 430Asp Glu Leu Lys Glu Lys Val Ala Asp Arg Val Ile Asp Lys Leu Thr435 440 445Asp Tyr Ala Pro Asn Leu Lys Ser Leu Ile Ile Gly Arg Arg Val Glu450 455 460Ser Pro Ala Glu Leu Ala Gln Arg Leu Gly Ser Tyr Asn Gly Asn Val465 470 475 480Tyr His Leu Asp Met Ser Leu Asp Gln Met Met Phe Leu Arg Pro Leu485 490 495Pro Glu Ile Ala Asn Tyr Gln Thr Pro Ile Lys Asn Leu Tyr Leu Thr500 505 510Gly Ala Gly Thr His Pro Gly Gly Ser Ile Ser Gly Met Pro Gly Arg515 520 525Asn Cys Ala Arg Val Phe Leu Lys Gln Gln Arg Arg Phe Trp530 535 540
<210>23<211>776<212>DNA<213>慢生根瘤菌(Bradyrhizobium sp.)<220>
<221>CDS<222>(1)..(774)<400>23atg cat gca gca acc gcc aag gct act gag ttc ggg gcc tct cgg cgc 48Met His Ala Ala Thr Ala Lys Ala Thr Glu Phe Gly Ala Ser Arg Arg1 5 10 15gac gat gcg agg cag cgc cgc gtc ggt ctc acg ctg gcc gcg gtc atc 96Asp Asp Ala Arg Gln Arg Arg Val Gly Leu Thr Leu Ala Ala Val Ile20 25 30atc gcc gcc tgg ctg gtg ctg cat gtc ggt ctg atg ttc ttc tgg ccg 144Ile Ala Ala Trp Leu Val Leu His Val Gly Leu Met Phe Phe Trp Pro35 40 45ctg acc ctt cac agc ctg ctg ccg gct ttg cct ctg gtg gtg ctg cag 192Leu Thr Leu His Ser Leu Leu Pro Ala Leu Pro Leu Val Val Leu Gln50 55 60acc tgg ctc tat gta ggc ctg ttc atc atc gcg cat gac tgc atg cac 240Thr Trp Leu Tyr Val Gly Leu Phe Ile Ile Ala His Asp Cys Met His65 70 75 80ggc tcg ctg gtg ccg ttc aag ccg cag gtc aac cgc cgt atc gga cag 288Gly Ser Leu Val Pro Phe Lys Pro Gln Val Asn Arg Arg Ile Gly Gln85 90 95ctc tgc ctg ttc ctc tat gcc ggg ttc tcc ttc gac gct ctc aat gtc 336Leu Cys Leu Phe Leu Tyr Ala Gly Phe Ser Phe Asp Ala Leu Asn Val100 105 110gag cac cac aag cat cac cgc cat ccc ggc acg gcc gag gat ccc gat 384Glu His His Lys His His Arg His Pro Gly Thr Ala Glu Asp Pro Asp115 120 125
ttc gac gag gtg ccg ccg cac ggc ttc tgg cac tgg ttc gcc agc ttt 432Phe Asp Glu Val Pro Pro His Gly Phe Trp His Trp Phe Ala Ser Phe130 135 140ttc ctg cac tat ttc ggc tgg aag cag gtc gcg atc atc gca gcc gtc 480Phe Leu His Tyr Phe Gly Trp Lys Gln Val Ala Ile Ile Ala Ala Val145 150 155 160tcg ctg gtt tat cag ctc gtc ttc gcc gtt ccc ttg cag aac atc ctg 528Ser Leu Val Tyr Gln Leu Val Phe Ala Val Pro Leu Gln Asn Ile Leu165 170 175ctg ttc tgg gcg ctg ccc ggg ctg ctg tcg gcg ctg cag ctg ttc acc 576Leu Phe Trp Ala Leu Pro Gly Leu Leu Ser Ala Leu Gln Leu Phe Thr180 185 190ttc ggc acc tat ctg ccg cac aag ccg gcc acg cag ccc ttc gcc gat 624Phe Gly Thr Tyr Leu Pro His Lys Pro Ala Thr Gln Pro Phe Ala Asp195 200 205cgc cac aac gcg cgg acg agc gaa ttt ccc gcg tgg ctg tcg ctg ctg 672Arg His Asn Ala Arg Thr Ser Glu Phe Pro Ala Trp Leu Ser Leu Leu210 215 220acc tgc ttc cac ttc ggc ttt cat cac gag cat cat ctg cat ccc gat 720Thr Cys Phe His Phe Gly Phe His His Glu His His Leu His Pro Asp225 230 235 240gcg ccg tgg tgg cgg ctg ccg gag atc aag cgg cgg gcc ctg gaa agg 768Ala Pro Trp Trp Arg Leu Pro Glu Ile Lys Arg Arg Ala Leu Glu Arg245 250 255cgt gac ta 776Arg Asp<210>24<211>258<212>PRT<213>慢生根瘤菌<400>24
Met His Ala Ala Thr Ala Lys Ala Thr Glu Phe Gly Ala Ser Arg Arg1 5 10 15Asp Asp Ala Arg Gln Arg Arg Val Gly Leu Thr Leu Ala Ala Val Ile20 25 30Ile Ala Ala Trp Leu Val Leu His Val Gly Leu Met Phe Phe Trp Pro35 40 45Leu Thr Leu His Ser Leu Leu Pro Ala Leu Pro Leu Val Val Leu Gln50 55 60Thr Trp Leu Tyr Val Gly Leu Phe Ile Ile Ala His Asp Cys Met His65 70 75 80Gly Ser Leu Val Pro Phe Lys Pro Gln Val Asn Arg Arg Ile Gly Gln85 90 95Leu Cys Leu Phe Leu Tyr Ala Gly Phe Ser Phe Asp Ala Leu Asn Val100 105 110Glu His His Lys His His Arg His Pro Gly Thr Ala Glu Asp Pro Asp115 120 125Phe Asp Glu Val Pro Pro His Gly Phe Trp His Trp Phe Ala Ser Phe130 135 140Phe Leu His Tyr Phe Gly Trp Lys Gln Val Ala Ile Ile Ala Ala Val145 150 155 160Ser Leu Val Tyr Gln Leu Val Phe Ala Val Pro Leu Gln Asn Ile Leu165 170 175
Leu Phe Trp Ala Leu Pro Gly Leu Leu Ser Ala Leu Gln Leu Phe Thr180 185 190Phe Gly Thr Tyr Leu Pro His Lys Pro Ala Thr Gln Pro Phe Ala Asp195 200 205Arg His Asn Ala Arg Thr Ser Glu Phe Pro Ala Trp Leu Ser Leu Leu210 215 220Thr Cys Phe His Phe Gly Phe His His Glu His His Leu His Pro Asp225 230 235 240Ala Pro Trp Trp Arg Leu Pro Glu Ile Lys Arg Arg Ala Leu Glu Arg245 250 255Arg Asp<210>25<211>777<212>DNA<213>念珠藻(Nostoc sp.)<220>
<221>CDS<222>(1)..(777)<400>25atg gtt cag tgt caa cea tca tct ctg cat tca gaa aaa ctg gtg tta 48Met Val Gln Cys Gln Pro Ser Ser Leu His Ser Glu Lys Leu Val Leu1 5 10 15ttg tca tcg aca atc aga gat gat aaa aat att aat aag ggt ata ttt 96Leu Ser Ser Thr Ile Arg Asp Asp Lys Asn Ile Asn Lys Gly Ile Phe20 25 30
att gcc tgc ttt atc tta ttt tta tgg gca att agt tta atc tta tta 144Ile Ala Cys Phe Ile Leu Phe Leu Trp Ala Ile Ser Leu Ile Leu Leu35 40 45ctc tca ata gat aca tcc ata att cat aag agc tta tta ggt ata gcc 192Leu Ser Ile Asp Thr Ser Ile Ile His Lys Ser Leu Leu Gly Ile Ala50 55 60atg ctt tgg cag acc ttc tta tat aca ggt tta ttt att act gct cat 240Met Leu Trp Gln Thr Phe Leu Tyr Thr Gly Leu Phe Ile Thr Ala His65 70 75 80gat gcc atg cac ggc gta gtt tat ccc aaa aat ccc aga ata aat aat 288Asp Ala Met His Gly Val Val Tyr Pro Lys Asn Pro Arg Ile Asn Asn85 90 95ttt ata ggt aag ctc act cta atc ttg tat gga cta ctc cct tat aaa 336Phe Ile Gly Lys Leu Thr Leu Ile Leu Tyr Gly Leu Leu Pro Tyr Lys100 105 110gat tta ttg aaa aaa cat tgg tta cac cac gga cat cct ggt act gat 384Asp Leu Leu Lys Lys His Trp Leu His His Gly His Pro Gly Thr Asp115 120 125tta gac cct gat tat tac aat ggt cat ccc caa aac ttc ttt ctt tgg 432Leu Asp Pro Asp Tyr Tyr Asn Gly His Pro Gln Asn Phe Phe Leu Trp130 135 140tat cta cat ttt atg aag tct tat tgg cga tgg acg caa att ttc gga 480Tyr Leu His Phe Met Lys Ser Tyr Trp Arg Trp Thr Gln Ile Phe Gly145 150 155 160tta gtg atg att ttt cat gga ctt aaa aat ctg gtg cat ata cca gaa 528Leu Val Met Ile Phe His Gly Leu Lys Asn Leu Val His Ile Pro Glu165 170 175aat aat tta att ata ttt tgg atg ata cct tct att tta agt tca gta 576Asn Asn Leu Ile Ile Phe Trp Met Ile Pro Ser Ile Leu Ser Ser Val180 185 190caa cta ttt tat ttt ggt aca ttt ttg cct cat aaa aag cta gaa ggt 624Gln Leu Phe Tyr Phe Gly Thr Phe Leu Pro His Lys Lys Leu Glu Gly195 200 205
ggt tat act aac ccc cat tgt gcg cgc agt atc cca tta cct ctt ttt 672Gly Tyr Thr Asn Pro His Cys Ala Arg Ser Ile Pro Leu Pro Leu Phe210 215 220tgg tct ttt gtt act tgt tat cac ttc ggc tac cac aag gaa cat cac 720Trp Ser Phe Val Thr Cys Tyr His Phe Gly Tyr His Lys Glu His His225 230 235 240gaa tac cct caa ctt cct tgg tgg aaa tta cct gaa gct cac aaa ata 768Glu Tyr Pro Gln Leu Pro Trp Trp Lys Leu Pro Glu Ala His Lys Ile245 250 255tct tta taa 777Ser Leu<210>26<211>258<212>PRT<213>念珠藻<400>26Met Val Gln Cys Gln Pro Ser Ser Leu His Ser Glu Lys Leu Val Leu1 5 10 15Leu Ser Ser Thr Ile Arg Asp Asp Lys Asn Ile Asn Lys Gly Ile Phe20 25 30Ile Ala Cys Phe Ile Leu Phe Leu Trp Ala Ile Ser Leu Ile Leu Leu35 40 45Leu Ser Ile Asp Thr Ser Ile Ile His Lys Ser Leu Leu Gly Ile Ala50 55 60Met Leu Trp Gln Thr Phe Leu Tyr Thr Gly Leu Phe Ile Thr Ala His65 70 75 80
Asp Ala Met His Gly Val Val Tyr Pro Lys Asn Pro Arg Ile Asn Asn85 90 95Phe Ile Gly Lys Leu Thr Leu Ile Leu Tyr Gly Leu Leu Pro Tyr Lys100 105 110Asp Leu Leu Lys Lys His Trp Leu His His Gly His Pro Gly Thr Asp115 120 125Leu Asp Pro Asp Tyr Tyr Asn Gly His Pro Gln Asn Phe Phe Leu Trp130 135 140Tyr Leu His Phe Met Lys Ser Tyr Trp Arg Trp Thr Gln Ile Phe Gly145 150 155 160Leu Val Met Ile Phe His Gly Leu Lys Asn Leu Val His Ile Pro Glu165 170 175Asn Asn Leu Ile Ile Phe Trp Met Ile Pro Ser Ile Leu Ser Ser Val180 185 190Gln Leu Phe Tyr Phe Gly Thr Phe Leu Pro His Lys Lys Leu Glu Gly195 200 205Gly Tyr Thr Asn Pro His Cys Ala Arg Ser Ile Pro Leu Pro Leu Phe210 215 220Trp Ser Phe Val Thr Cys Tyr His Phe Gly Tyr His Lys Glu His His225 230 235 240Glu Tyr Pro Gln Leu Pro Trp Trp Lys Leu Pro Glu Ala His Lys Ile245 250 255
Ser Leu<210>27<211>789<212>DNA<213>點(diǎn)形念珠藻(Nostoc punctiforme)<220>
<221>CDS<222>(1)..(789)<400>27ttg aat ttt tgt gat aaa cca gtt agc tat tat gtt gca ata gag caa 48Leu Asn Phe Cys Asp Lys Pro Val Ser Tyr Tyr Val Ala Ile Glu Gln1 5 10 15tta agt gct aaa gaa gat act gtt tgg ggg ctg gtg att gtc ata gta 96Leu Ser Ala Lys Glu Asp Thr Val Trp Gly Leu Val Ile Val Ile Val20 25 30att att agt ctt tgg gta gct agt ttg gct ttt tta cta gct att aat 144Ile Ile Ser Leu Trp Val Ala Ser Leu Ala Phe Leu Leu Ala Ile Asn35 40 45tat gcc aaa gtc cca att tgg ttg ata cct att gca ata gtt tgg caa 192Tyr Ala Lys Val Pro Ile Trp Leu Ile Pro Ile Ala Ile Val Trp Gln50 55 60atg ttc ctt tat aca ggg cta ttt att act gca cat gat gct atg cat 240Met Phe Leu Tyr Thr Gly Leu Phe Ile Thr Ala His Asp Ala Met His65 70 75 80ggg tca gtt tat cgt aaa aat ccc aaa att aat aat ttt atc ggt tca 288Gly Ser Val Tyr Arg Lys Asn Pro Lys Ile Asn Asn Phe Ile Gly Ser85 90 95cta gct gta gcg ctt tac gct gtg ttt cca tat caa cag atg tta aag 336Leu Ala Val Ala Leu Tyr Ala Val Phe Pro Tyr Gln Gln Met Leu Lys100 105 110aat cat tgc tta cat cat cgt cat cct gct agc gaa gtt gac cca gat 384
Asn His Cys Leu His His Arg His Pro Ala Ser Glu Val Asp Pro Asp115 120 125ttt cat gat ggt aag aga aca aac gct att ttc tgg tat ctc cat tte 432Phe His Asp Gly Lys Arg Thr Asn Ala Ile Phe Trp Tyr Leu His Phe130 135 140atg ata gaa tac tcc agt tgg caa cag tta ata gta cta act atc cta 480Met Ile Glu Tyr Ser Ser Trp Gln Gln Leu Ile Val Leu Thr Ile Leu145 150 155 160ttt aat tta gct aaa tac gtt ttg cac atc cat caa ata aat ctc atc 528Phe Asn Leu Ala Lys Tyr Val Leu His Ile His Gln Ile Asn Leu Ile165 170 175tta ttt tgg agt att cct cca att tta agt tcc att caa ctg ttt tat 576Leu Phe Trp Ser Ile Pro Pro Ile Leu Ser Ser Ile Gln Leu Phe Tyr180 185 190ttc gga aca ttt ttg cct cat cga gaa ccc aag aaa gga tat gtt tat 624Phe Gly Thr Phe Leu Pro His Arg Glu Pro Lys Lys Gly Tyr Val Tyr195 200 205ccc cat tgc agc caa aca ata aaa ttg cca act ttt ttg tca ttt atc 672Pro His Cys Ser Gln Thr Ile Lys Leu Pro Thr Phe Leu Ser Phe Ile210 215 220gct tgc tac cac ttt ggt tat cat gaa gaa cat cat gag tat ccc cat 720Ala Cys Tyr His Phe Gly Tyr His Glu Glu His His Glu Tyr Pro His225 230 235 240gta cct tgg tgg caa ctt cca tct gta tat aag cag aga gta ttc aac 768Val Pro Trp Trp Gln Leu Pro Ser Val Tyr Lys Gln Arg Val Phe Asn245 250 255aat tca gta acc aat tcg taa 789Asn Ser Val Thr Asn Ser260<210>28<211>262<212>PRT<213>點(diǎn)形念珠藻
<400>28Leu Asn Phe Cys Asp Lys Pro Val Ser Tyr Tyr Val Ala Ile Glu Gln1 5 10 15Leu Ser Ala Lys Glu Asp Thr Val Trp Gly Leu Val Ile Val Ile Val20 25 30Ile Ile Ser Leu Trp Val Ala Ser Leu Ala Phe Leu Leu Ala Ile Asn35 40 45Tyr Ala Lys Val Pro Ile Trp Leu Ile Pro Ile Ala Ile Val Trp Gln50 55 60Met Phe Leu Tyr Thr Gly Leu Phe Ile Thr Ala His Asp Ala Met His65 70 75 80Gly Ser Val Tyr Arg Lys Asn Pro Lys Ile Asn Asn Phe Ile Gly Ser85 90 95Leu Ala Val Ala Leu Tyr Ala Val Phe Pro Tyr Gln Gln Met Leu Lys100 105 110Asn His Cys Leu His His Arg His Pro Ala Ser Glu Val Asp Pro Asp115 120 125Phe His Asp Gly Lys Arg Thr Asn Ala Ile Phe Trp Tyr Leu His Phe130 135 140Met Ile Glu Tyr Ser Ser Trp Gln Gln Leu Ile Val Leu Thr Ile Leu145 150 155 160Phe Asn Leu Ala Lys Tyr Val Leu His Ile His Gln Ile Asn Leu Ile
165 170 175Leu Phe Trp Ser Ile Pro Pro Ile Leu Ser Ser Ile Gln Leu Phe Tyr180 185 190Phe Gly Thr Phe Leu Pro His Arg Glu Pro Lys Lys Gly Tyr Val Tyr195 200 205Pro His Cys Ser Gln Thr Ile Lys Leu Pro Thr Phe Leu Ser Phe Ile210 215 220Ala Cys Tyr His Phe Gly Tyr His Glu Glu His His Glu Tyr Pro His225 230 235 240Val Pro Trp Trp Gln Leu Pro Ser Val Tyr Lys Gln Arg Val Phe Asn245 250 255Asn Ser Val Thr Asn Ser260<210>29<211>762<212>DNA<213>點(diǎn)形念珠藻<220>
<221>CDS<222>(1)..(762)<400>29gtg atc cag tta gaa caa cca ctc agt cat caa gca aaa ctg act cca 48Val Ile Gln Leu Glu Gln Pro Leu Ser His Gln Ala Lys Leu Thr Pro1 5 10 15gta ctg aga agt aaa tct cag ttt aag ggg ctt ttc att gct att gtc 96Val Leu Arg Ser Lys Ser Gln Phe Lys Gly Leu Phe Ile Ala Ile Val
20 25 30att gtt agc gca tgg gtc att agc ctg agt tta tta ctt tcc ctt gac 144Ile Val Ser Ala Trp Val Ile Ser Leu Ser Leu Leu Leu Ser Leu Asp35 40 45atc tca aag cta aaa ttt tgg atg tta ttg cct gtt ata cta tgg caa 192Ile Ser Lys Leu Lys Phe Trp Met Leu Leu Pro Val Ile Leu Trp Gln50 55 60aca ttt tta tat acg gga tta ttt att aca tct cat gat gcc atg cat 240Thr Phe Leu Tyr Thr Gly Leu Phe Ile Thr Ser His Asp Ala Met His65 70 75 80ggc gta gta ttt ccc caa aac acc aag att aat cat ttg att gga aca 288Gly Val Val Phe Pro Gln Asn Thr Lys Ile Asn His Leu Ile Gly Thr85 90 95ttg acc cta tcc ctt tat ggt ctt tta cca tat caa aaa cta ttg aaa 336Leu Thr Leu Ser Leu Tyr Gly Leu Leu Pro Tyr Gln Lys Leu Leu Lys100 105 110aaa cat tgg tta cac cac cac aat cca gca agc tca ata gac ccg gat 384Lys His Trp Leu His His His Asn Pro Ala Ser Ser Ile Asp Pro Asp115 120 125ttt cac aat ggt aaa cac caa agt ttc ttt gct tgg tat ttt cat ttt 432Phe His Asn Gly Lys His Gln Ser Phe Phe Ala Trp Tyr Phe His Phe130 135 140atg aaa ggt tac tgg agt tgg ggg caa ata att gcg ttg act att att 480Met Lys Gly Tyr Trp Ser Trp Gly Gln Ile Ile Ala Leu Thr Ile Ile145 150 155 160tat aac ttt gct aaa tac ata ctc cat atc cca agt gat aat cta act 528Tyr Asn Phe Ala Lys Tyr Ile Leu His Ile Pro Ser Asp Asn Leu Thr165 170 175tac ttt tgg gtg cta ccc tcg ctt tta agt tca tta caa tta ttc tat 576Tyr Phe Trp Val Leu Pro Ser Leu Leu Ser Ser Leu Gln Leu Phe Tyr180 185 190ttt ggt act ttt tta ccc cat agt gaa cca ata ggg ggt tat gtt cag 624Phe Gly Thr Phe Leu Pro His Ser Glu Pro Ile Gly Gly Tyr Val Gln
195 200 205cct cat tgt gcc caa aca att agc cgt cct att tgg tgg tca ttt atc 672Pro His Cys Ala Gln Thr Ile Ser Arg Pro Ile Trp Trp Ser Phe Ile210 215 220acg tgc tat cat ttt ggc tac cac gag gaa cat cac gaa tat cct cat 720Thr Cys Tyr His Phe Gly Tyr His Glu Glu His His Glu Tyr Pro His225 230 235 240att tct tgg tgg cag tta cca gaa att tac aaa gca aaa tag 762Ile Ser Trp Trp Gln Leu Pro Glu Ile Tyr Lys Ala Lys245 250<210>30<211>253<212>PRT<213>點(diǎn)形念珠藻<400>30Val IIe Gln Leu Glu Gln Pro Leu Ser His Gln Ala Lys Leu Thr Pro1 5 10 15Val Leu Arg Ser Lys Ser Gln Phe Lys Gly Leu Phe Ile Ala Ile Val20 25 30Ile Val Ser Ala Trp Val Ile Ser Leu Ser Leu Leu Leu Ser Leu Asp35 40 45Ile Ser Lys Leu Lys Phe Trp Met Leu Leu Pro Val Ile Leu Trp Gln50 55 60Thr Phe Leu Tyr Thr Gly Leu Phe Ile Thr Ser His Asp Ala Met His65 70 75 80Gly Val Val Phe Pro Gln Asn Thr Lys Ile Asn His Leu Ile Gly Thr85 90 95
Leu Thr Leu Ser Leu Tyr Gly Leu Leu Pro Tyr Gln Lys Leu Leu Lys100 105 110Lys His Trp Leu His His His Asn Pro Ala Ser Ser Ile Asp Pro Asp115 120 125Phe His Asn Gly Lys His Gln Ser Phe Phe Ala Trp Tyr Phe His Phe130 135 140Met Lys Gly Tyr Trp Ser Trp Gly Gln Ile Ile Ala Leu Thr Ile Ile145 150 155 160Tyr Asn Phe Ala Lys Tyr Ile Leu His Ile Pro Ser Asp Asn Leu Thr165 170 175Tyr Phe Trp Val Leu Pro Ser Leu Leu Ser Ser Leu Gln Leu Phe Tyr180 185 190Phe Gly Thr Phe Leu Pro His Ser Glu Pro Ile Gly Gly Tyr Val Gln195 200 205Pro His Cys Ala Gln Thr Ile Ser Arg Pro Ile Trp Trp Ser Phe Ile210 215 220Thr Cys Tyr His Phe Gly Tyr His Glu Glu His His Glu Tyr Pro His225 230 235 240Ile Ser Trp Trp Gln Leu Pro Glu Ile Tyr Lys Ala Lys245 250<210>31<211>1608
<212>DNA<213>雨生紅球藻<220>
<221>CDS<222>(3)..(971)<400>31ct aca ttt cac aag ccc gtg agc ggt gca agc gct ctg ccc cac atc47Thr Phe His Lys Pro Val Ser Gly Ala Ser Ala Leu Pro His Ile1 5 10 15ggc cca cct cct cat ctc cat cgg tca ttt gct gct acc acg atg ctg 95Gly Pro Pro Pro His Leu His Arg Ser Phe Ala Ala Thr Thr Met Leu20 25 30tcg aag ctg cag tca atc agc gtc aag gcc cgc cgc gtt gaa cta gcc 143Ser Lys Leu Gln Ser Ile Ser Val Lys Ala Arg Arg Val Glu Leu Ala35 40 45cgc gac atc acg cgg ccc aaa gtc tgc ctg cat gct cag cgg tgc tcg 191Arg Asp Ile Thr Arg Pro Lys Val Cys Leu His Ala Gln Arg Cys Ser50 55 60tta gtt cgg ctg cga gtg gca gca cca cag aca gag gag gcg ctg gga 239Leu Val Arg Leu Arg Val Ala Ala Pro Gln Thr Glu Glu Ala Leu Gly65 70 75acc gtg cag gct gcc ggc gcg ggc gat gag cac agc gcc gat gta gca 287Thr Val Gln Ala Ala Gly Ala Gly Asp Glu His Ser Ala Asp Val Ala80 85 90 95ctc cag cag ctt gac cgg gct atc gca gag cgt cgt gcc cgg cgc aaa 335Leu Gln Gln Leu Asp Arg Ala Ile Ala Glu Arg Arg Ala Arg Arg Lys100 105 110cgg gag cag ctg tca tac cag gct gcc gcc att gca gca tca att ggc 383Arg Glu Gln Leu Ser Tyr Gln Ala Ala Ala Ile Ala Ala Ser Ile Gly115 120 125gtg tca ggc att gcc atc ttc gcc acc tac ctg aga ttt gcc atg cac 431Val Ser Gly Ile Ala Ile Phe Ala Thr Tyr Leu Arg Phe Ala Met His130 135 140
atg acc gtg ggc ggc gca gtg cca tgg ggt gaa gtg gct ggc act ctc 479Met Thr Val Gly Gly Ala Val Pro Trp Gly Glu Val Ala Gly Thr Leu145 150 155ctc ttg gtg gtt ggt ggc gcg ctc ggc atg gag atg tat gcc cgc tat 527Leu Leu Val Val Gly Gly Ala Leu Gly Met Glu Met Tyr Ala Arg Tyr160 165 170 175gca cac aaa gcc atc tgg cat gag tcg cct ctg ggc tgg ctg ctg cac 575Ala His Lys Ala Ile Trp His Glu Ser Pro Leu Gly Trp Leu Leu His180 185 190aag agc cac cac aca cct cgc act gga ccc ttt gaa gcc aac gac ttg 623Lys Ser His His Thr Pro Arg Thr Gly Pro Phe Glu Ala Asn Asp Leu195 200 205ttt gca atc atc aat gga ctg ccc gcc atg ctc ctg tgt acc ttt ggc 671Phe Ala Ile Ile Asn Gly Leu Pro Ala Met Leu Leu Cys Thr Phe Gly210 215 220ttc tgg ctg ccc aac gtc ctg ggg gcg gcc tgc ttt gga gcg ggg ctg 719Phe Trp Leu Pro Asn Val Leu Gly Ala Ala Cys Phe Gly Ala Gly Leu225 230 235ggc atc acg cta tac ggc atg gca tat atg ttt gta cac gat ggc ctg 767Gly Ile Thr Leu Tyr Gly Met Ala Tyr Met Phe Val His Asp Gly Leu240 245 250 255gtg cac agg cgc ttt ccc acc ggg ccc atc gct ggc ctg ccc tac atg 815Val His Arg Arg Phe Pro Thr Gly Pro Ile Ala Gly Leu Pro Tyr Met260 265 270aag cgc ctg aca gtg gcc cac cag cta cac cac agc ggc aag tac ggt 863Lys Arg Leu Thr Val Ala His Gln Leu His His Ser Gly Lys Tyr Gly275 280 285ggc gcg ccc tgg ggt atg ttc ttg ggt cca cag gag ctg cag cac att 911Gly Ala Pro Trp Gly Met Phe Leu Gly Pro Gln Glu Leu Gln His Ile290 295 300cca ggt gcg gcg gag gag gtg gag cga ctg gtc ctg gaa ctg gac tgg 959Pro Gly Ala Ala Glu Glu Val Glu Arg Leu Val Leu Glu Leu Asp Trp305 310 315
tcc aag cgg tag ggtgcggaac caggcacgct ggtttcacac ctcatgcctg 1011Ser Lys Arg320tgataaggtg tggctagagc gatgcgtgtg agacgggtat gtcacggtcg actggtctga 1071tggccaatgg catcggccat gtctggtcat cacgggctgg ttgcctgggt gaaggtgatg 1131cacatcatca tgtgcggttg gaggggctgg cacagtgtgg gctgaactgg agcagttgtc 1191caggctggcg ttgaatcagt gagggtttgt gattggcggt tgtgaagcaa tgactccgcc 1251catattctat ttgtgggagc tgagatgatg gcatgcttgg gatgtgcatg gatcatggta 1311gtgcagcaaa ctatattcac ctagggctgt tggtaggatc aggtgaggcc ttgcacattg 1371catgatgtac tcgtcatggt gtgttggtga gaggatggat gtggatggat gtgtattctc 1431agacgtagac cttgactgga ggcttgatcg agagagtggg ccgtattctt tgagagggga 1491ggctcgtgcc agaaatggtg agtggatgac tgtgacgctg tacattgcag gcaggtgaga 1551tgcactgtct cgattgtaaa atacattcag atgcaaaaaa aaaaaaaaaa aaaaaaa 1608<210>32<211>322<212>PRT<213>雨生紅球藻<400>32Thr Phe His Lys Pro Val Ser Gly Ala Ser Ala Leu Pro His Ile Gly1 5 10 15Pro Pro Pro His Leu His Arg Ser Phe Ala Ala Thr Thr Met Leu Ser20 25 30Lys Leu Gln Ser Ile Ser Val Lys Ala Arg Arg Val Glu Leu Ala Arg35 40 45
Asp Ile Thr Arg Pro Lys Val Cys Leu His Ala Gln Arg Cys Ser Leu50 55 60Val Arg Leu Arg Val Ala Ala Pro Gln Thr Glu Glu Ala Leu Gly Thr65 70 75 80Val Gln Ala Ala Gly Ala Gly Asp Glu His Ser Ala Asp Val Ala Leu85 90 95Gln Gln Leu Asp Arg Ala Ile Ala Glu Arg Arg Ala Arg Arg Lys Arg100 105 110Glu Gln Leu Ser Tyr Gln Ala Ala Ala Ile Ala Ala Ser Ile Gly Val115 120 125Ser Gly Ile Ala Ile Phe Ala Thr Tyr Leu Arg Phe Ala Met His Met130 135 140Thr Val Gly Gly Ala Val Pro Trp Gly Glu Val Ala Gly Thr Leu Leu145 150 155 160Leu Val Val Gly Gly Ala Leu Gly Met Glu Met Tyr Ala Arg Tyr Ala165 170 175His Lys Ala Ile Trp His Glu Ser Pro Leu Gly Trp Leu Leu His Lys180 185 190Ser His His Thr Pro Arg Thr Gly Pro Phe Glu Ala Asn Asp Leu Phe195 200 205Ala Ile Ile Asn Gly Leu Pro Ala Met Leu Leu Cys Thr Phe Gly Phe210 215 220
Trp Leu Pro Asn Val Leu Gly Ala Ala Cys Phe Gly Ala Gly Leu Gly225 230 235 240Ile Thr Leu Tyr Gly Met Ala Tyr Met Phe Val His Asp Gly Leu Val245 250 255His Arg Arg Phe Pro Thr Gly Pro Ile Ala Gly Leu Pro Tyr Met Lys260 265 270Arg Leu Thr Val Ala His Gln Leu His His Ser Gly Lys Tyr Gly Gly275 280 285Ala Pro Trp Gly Met Phe Leu Gly Pro Gln Glu Leu Gln His Ile Pro290 295 300Gly Ala Ala Glu Glu Val Glu Arg Leu Val Leu Glu Leu Asp Trp Ser305 310 315 320Lys Arg<210>33<211>528<212>DNA<213>嗜夏孢歐文氏菌(Erwinia uredovora)<220>
<221>CDS<222>(1)..(528)<400>33atg ttg tgg att tgg aat gcc ctg atc gtt ttc gtt acc gtg att ggc 48Met Leu Trp Ile Trp Asn Ala Leu Ile Val Phe Val Thr Val Ile Gly1 5 10 15
atg gaa gtg att gct gca ctg gca cac aaa tac atc atg cac ggc tgg 96Met Glu Val Ile Ala Ala Leu Ala His Lys Tyr Ile Met His Gly Trp20 25 30ggt tgg gga tgg cat ctt tca cat cat gaa ccg cgt aaa ggt gcg ttt 144Gly Trp Gly Trp His Leu Ser His His Glu Pro Arg Lys Gly Ala Phe35 40 45gaa gtt aac gat ctt tat gcc gtg gtt ttt gct gca tta tcg atc ctg 192Glu Val Asn Asp Leu Tyr Ala Val Val Phe Ala Ala Leu Ser Ile Leu50 55 60ctg att tat ctg ggc agt aca gga atg tgg ccg ctc cag tgg att ggc 240Leu Ile Tyr Leu Gly Ser Thr Gly Met Trp Pro Leu Gln Trp Ile Gly65 70 75 80gca ggt atg acg gcg tat gga tta ctc tat ttt atg gtg cac gac ggg 288Ala Gly Met Thr Ala Tyr Gly Leu Leu Tyr Phe Met Val His Asp Gly85 90 95ctg gtg cat caa cgt tgg cca ttc cgc tat att cca cgc aag ggc tac 336Leu Val His Gln Arg Trp Pro Phe Arg Tyr Ile Pro Arg Lys Gly Tyr100 105 110ctc aaa cgg ttg tat atg gcg cac cgt atg cat cac gcc gtc agg ggc 384Leu Lys Arg Leu Tyr Met Ala His Arg Met Hi s His Ala Val Arg Gly115 120 125aaa gaa ggt tgt gtt tct ttt ggc ttc ctc tat gcg ccg ccc ctg tca 432Lys Glu Gly Cys Val Ser Phe Gly Phe Leu Tyr Ala Pro Pro Leu Ser130 135 140aaa ctt cag gcg acg ctc cgg gaa aga cat ggc gct aga gcg ggc gct 480Lys Leu Gln Ala Thr Leu Arg Glu Arg His Gly Ala Arg Ala Gly Ala145 150 155 160gcc aga gat gcg cag ggc ggg gag gat gag ccc gca tcc ggg aag taa 528Ala Arg Asp Ala Gln Gly Gly Glu Asp Glu Pro Ala Ser Gly Lys165 170 175<210>34<211>175<212>PRT
<213>嗜夏孢歐文氏菌<400>34Met Leu Trp Ile Trp Asn Ala Leu Ile Val Phe Val Thr Val Ile Gly1 5 10 15Met Glu Val Ile Ala Ala Leu Ala His Lys Tyr Ile Met His Gly Trp20 25 30Gly Trp Gly Trp His Leu Ser His His Glu Pro Arg Lys Gly Ala Phe35 40 45Glu Val Asn Asp Leu Tyr Ala Val Val Phe Ala Ala Leu Ser Ile Leu50 55 60Leu Ile Tyr Leu Gly Ser Thr Gly Met Trp Pro Leu Gln Trp Ile Gly65 70 75 80Ala Gly Met Thr Ala Tyr Gly Leu Leu Tyr Phe Met Val His Asp Gly85 90 95Leu Val His Gln Arg Trp Pro Phe Arg Tyr Ile Pro Arg Lys Gly Tyr100 105 110Leu Lys Arg Leu Tyr Met Ala His Arg Met His His Ala Val Arg Gly115 120 125Lys Glu Gly Cys Val Ser Phe Gly Phe Leu Tyr Ala Pro Pro Leu Ser130 135 140Lys Leu Gln Ala Thr Leu Arg Glu Arg His Gly Ala Arg Ala Gly Ala145 150 155 160
Ala Arg Asp Ala Gln Gly Gly Glu Asp Glu Pro Ala Ser Gly Lys165 170 175<210>35<211>1520<212>DNA<213>人工序列<220>
<223>啟動子<400>35ctcgagtacc gaggcggaac ggcaggaatg tttccctctc ttttagaggg caattcttta 60tccaatgtca tgttgatgct agatatttct gtctcttata ataaggcgaa tacccatttt120tgaattgaag ttgagataaa aaaaaagggg gcccaatttg tcaacgccaa agagtcaagc180tttttctttg gctttagccg aacaatctaa gacttattgt ttttgaagat atttgacctt240ttctagatat tccttcaagt aaagcttttt tcgagttttt tttttttttc tttgtgaagg300atttattgtt attggtatcc attttttatt ggaagacaag ataagttaat attgattttg360cttaaagatt aaaaggaaat cagaaaacga caataaaaaa tgtaacggac aaactatggt420gtcgattata agtctaaatc cttaaaaaat gacaacgagt tgctttcctc tgaaaacaat480tcttttgtct ttgcaagaaa ggtttctttt ttgtttgctt gcattactta aacatcaaat540caaatgaaag gaataaagca gatttgaggg cgaataagga ttttctggtc aacaagatgt600gagtgacacc taaggaacta aatgccattc atttgtttta aaacgacatc aaagattgat660gatcaacagg attgagagag agaaaaagaa ctcgtgtcat ttatttctgt tgactgaaat720tttatattta gaaaaaatgt caaatctata gctttagcta tattacataa catttgaaat780aataataata aaaaaagaca cattagagac acttttcaaa ctctaaataa ctgtctataa840acacaaagaa aacaaagacc tctataacaa cttattagat ttttctcgta cttttgtcta900aagatgatgt attcttgtta tcccacactt ctttcatttg ttcttgatgc tactaaatat960
acaaaatttc ttttttgcaa gagatattat tccaaaaatt ttcaaaaaga aatttttttc 1020acaatagcag ttgatcgtgt aacccaaaga ggttctttgt tattttgcac ttccgctttg 1080cggtgatgca tattcaaagt aatatatgga ataaacaacg tgtttaagca tgaaagaaag 1140gaaacaaagg ccgctttgaa caaatgcata atatttcaga caaaaatgat ctaaagcaag 1200cagtaaatca aacaagaaac attgctgatt cgcgttagaa aacgataaaa gtctaataag 1260ccactaagta tacttcaatg aactttttgt atgcttatgg tccaatcaga ccaataattt 1320gtgaccattc ctgaggtggc tttggtgatg cggaaacaga aaaaaatttt ctcaccaatc 1380gatttaaaaa acaatttctg ctttgaacca aaactttttt tttctcttta atcattaact 1440ttatcaagta tgtacctacc ctcaaagtcc tcactcaagc acaattatgc taacattgtt 1500ccaccttctc tttagaaatg 1520<210>36<211>16245<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)
<223>n為a，c，g，或t<400>36ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260
ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacct2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580
tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900
tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220
attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540
ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860
ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180
gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500
tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt aatctataca 10800atgctccata gactcacatt gatattgtcg aagatttcga tgctgactta gtagagcaac 10860tacaaaagtt agcagagaag catgatttct taatctttga agaccgcaag tttgcagata 10920tcggtatgtg aattctatct attttttttc tgatgtgtgc atggatgact catgatcata 10980ttcttaggta atactgtcaa gcatcaatat ggcaagggcg tttacaagat tgcttcttgg 11040tctcatatta ctaatgctca cacagttcct ggagaaggta ttatcaaggg acttgccgaa 11100gtcggcctcc ctcttggtcg tggcttgctt ttgctagcag aaatgtcatc tcaaggtgca 11160ttaactaagg gtatttacac tgccgaatct gtcaatatgg ctcgccgcaa caaagatttc 11220gtttttggct ttattgcaca acacaaaatg aatcagtatg atgatgagga ttttgttgtc 11280atgtcgcctg aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 11340cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 11400aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 11460acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 11520ttgggccaaa gacaaaaggg cgacattcaa ccgattgagg gagggaaggt aaatattgac 11580ggaaattatt cattaaaggt gaattatcac cgtcaccgac ttgagccatt tgggaattag 11640agccagcaaa atcaccagta gcaccattac cattagcaag gccggaaacg tcaccaatga 11700aaccatcgat agcagcaccg taatcagtag cgacagaatc aagtttgcct ttagcgtcag 11760actgtagcgc gttttcatcg gcattttcgg tcatagcccc cttattagcg tttgccatct 11820
tttcataatc aaaatcaccg gaaccagagc caccaccgga accgcctccc tcagagccgc 11880caccctcaga accgccaccc tcagagccac caccctcaga gccgccacca gaaccaccac 11940cagagccgcc gccagcattg acaggaggcc cgatctagta acatagatga caccgcgcgc 12000gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat 12060tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt 12120attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca 12180ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatcggggat catccgggtc 12240tgtggcggga actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc 12300ttgcctgggc agtcgccgcc gacgccgttg atgtggacgc cgggcccgat catattgtcg 12360ctcaggatcg tggcgttgtg cttgtcggcc gttgctgtcg taatgatatc ggcaccttcg 12420accgcctgtt ccgcagagat cccgtgggcg aagaactcca gcatgagatc cccgcgctgg 12480aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg 12540gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac 12600cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat 12660cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt 12720cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc 12780cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat 12840cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg cgccttgagc ctggcgaaca 12900gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg 12960cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg 13020tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg 13080caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt 13140
cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca 13200gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct 13260tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc 13320cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac 13380ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc agatccggtg cagattattt 13440ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt 13500ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg 13560cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga 13620gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc 13680atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc 13740ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga 13800aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg 13860tccatttgta tgtgcatgcc aaccacaggg ttccccagat ctggcgccgg ccagcgagac 13920gagcaagatt ggccgccgcc cgaaacgatc cgacagcgcg cccagcacag gtgcgcaggc 13980aaattgcacc aacgcataca gcgccagcag aatgccatag tgggcggtga cgtcgttcga 14040gtgaaccaga tcgcgcagga ggcccggcag caccggcata atcaggccga tgccgacagc 14100gtcgagcgcg acagtgctca gaattacgat caggggtatg ttgggtttca cgtctggcct 14160ccggaccagc ctccgctggt ccgattgaac gcgcggattc tttatcactg ataagttggt 14220ggacatatta tgtttatcag tgataaagtg tcaagcatga caaagttgca gccgaataca 14280gtgatccgtg ccgccctgga cctgttgaac gaggtcggcg tagacggtct gacgacacgc 14340aaactggcgg aacggttggg ggttcagcag ccggcgcttt actggcactt caggaacaag 14400cgggcgctgc tcgacgcact ggccgaagcc atgctggcgg agaatcatac gcattcggtg 14460
ccgagagccg acgacgactg gcgctcattt ctgatcggga atgcccgcag cttcaggcag 14520gcgctgctcg cctaccgcga tggcgcgcgc atccatgccg gcacgcgacc gggcgcaccg 14580cagatggaaa cggccgacgc gcagcttcgc ttcctctgcg aggcgggttt ttcggccggg 14640gacgccgtca atgcgctgat gacaatcagc tacttcactg ttggggccgt gcttgaggag 14700caggccggcg acagcgatgc cggcgagcgc ggcggcaccg ttgaacaggc tccgctctcg 14760ccgctgttgc gggccgcgat agacgccttc gacgaagccg gtccggacgc agcgttcgag 14820cagggactcg cggtgattgt cgatggattg gcgaaaagga ggctcgttgt caggaacgtt 14880gaaggaccga gaaagggtga cgattgatca ggaccgctgc cggagcgcaa cccactcact 14940acagcagagc catgtagaca acatcccctc cccctttcca ccgcgtcaga cgcccgtagc 15000agcccgctac gggctttttc atgccctgcc ctagcgtcca agcctcacgg ccgcgctcgg 15060cctctctggc ggccttctgg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 15120tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 15180aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 15240gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 15300aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 15360ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 15420tgtccgcctt tctcccttcg ggaagcgtgg cgcttttccg ctgcataacc ctgcttcggg 15480gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc 15540caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt 15600gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg 15660gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt aacagatgag 15720ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc tatcaaggtg 15780
tactgccttc cagacgaacg aagagcgatt gaggaaaagg cggcggcggc cggcatgagc 15840ctgtcggcct acctgctggc cgtcggccag ggctacaaaa tcacgggcgt cgtggactat 15900gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg gccgcctggg cggcctgctg 15960aaactctggc tcaccgacga cccgcgcacg gcgcggttcg gtgatgccac gatcctcgcc 16020ctgctggcga agatcgaaga gaagcaggac gagcttggca aggtcatgat gggcgtggtc 16080cgcccgaggg cagagccatg acttttttag ccgctaaaac ggccgggggg tgcgcgtgat 16140tgccaagcac gtccccatgc gctccatcaa gaagagcgac ttcgcggagc tggtgaagta 16200catcaccgac gagcaaggca agaccgagcg cctttgcgac gctca 16245<210>37<211>17877<212>DNA<213>人工序列<220>
<223>啟動子<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>37ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60
aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380
agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700
attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaa3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcg3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgc3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020
tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340
ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660
cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980
cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300
ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620
taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt ttttcgagtt 10800tttttttttt ttctttgtga aggatttatt gttattggta tccatttttt attggaagac 10860aagataagtt aatattgatt ttgcttaaag attaaaagga aatcagaaaa cgacaataaa 10920aaatgtaacg gacaaactat ggtgtcgatt ataagtctaa atccttaaaa aatgacaacg 10980agttgctttc ctctgaaaac aattcttttg tctttgcaag aaaggtttct tttttgtttg 11040cttgcattac ttaaacatca aatcaaatga aaggaataaa gcagatttga gggcgaataa 11100ggattttctg gtcaacaaga tgtgagtgac acctaaggaa ctaaatgcca ttcatttgtt 11160ttaaaacgac atcaaagatt gatgatcaac aggattgaga gagagaaaaa gaactcgtgt 11220catttatttc tgttgactga aattttatat ttagaaaaaa tgtcaaatct atagctttag 11280ctatattaca taacatttga aataataata ataaaaaaag acacattaga gacacttttc 11340aaactctaaa taactgtcta taaacacaaa gaaaacaaag acctctataa caacttatta 11400gatttttctc gtacttttgt ctaaagatga tgtattcttg ttatcccaca cttctttcat 11460ttgttcttga tgctactaaa tatacaaaat ttcttttttg caagagatat tattccaaaa 11520attttcaaaa agaaattttt ttcacaatag cagttgatcg tgtaacccaa agaggttctt 11580tgttattttg cacttccgct ttgcggtgat gcatattcaa agtaatatat ggaataaaca 11640acgtgtttaa gcatgaaaga aaggaaacaa aggccgcttt gaacaaatgc ataatatttc 11700agacaaaaat gatctaaagc aagcagtaaa tcaaacaaga aacattgctg attcgcgtta 11760gaaaacgata aaagtctaat aagccactaa gtatacttca atgaactttt tgtatgctta 11820tggtccaatc agaccaataa tttgtgacca ttcctgaggt ggctttggtg atgcggaaac 11880agaaaaaaat tttctcacca atcgatttaa aaaacaattt ctgctttgaa ccaaaacttt 11940
ttttttctct ttaatcatta actttatcaa gtatgtacct accctcaaag tcctcactca 12000agcacaatta tgctaacatt gttccacctt ctctttagaa atgctgtcga agctgcagtc 12060aatcagcgtc aaggcccgcc gcgttgaact agcccgcgac atcacgcggc ccaaagtctg 12120cctgcatgct cagcggtgct cgttagttcg gctgcgagtg gcagcaccac agacagagga 12180ggcgctggga accgtgcagg ctgccggcgc gggcgatgag cacagcgccg atgtagcact 12240ccagcagctt gaccgggcta tcgcagagcg tcgtgcccgg cgcaaacggg agcagctgtc 12300ataccaggct gccgccattg cagcatcaat tggcgtgtca ggcattgcca tcttcgccac 12360ctacctgaga tttgccatgc acatgaccgt gggcggcgca gtgccatggg gtgaagtggc 12420tggcactctc ctcttggtgg ttggtggcgc gctcggcatg gagatgtatg cccgctatgc 12480acacaaagcc atctggcatg agtcgcctct gggctggctg ctgcacaaga gccaccacac 12540acctcgcact ggaccctttg aagccaacga cttgtttgca atcatcaatg gactgcccgc 12600catgctcctg tgtacctttg gcttctggct gcccaacgtc ctgggggcgg cctgctttgg 12660agcggggctg ggcatcacgc tatacggcat ggcatatatg tttgtacacg atggcctggt 12720gcacaggcgc tttcccaccg ggcccatcgc tggcctgccc tacatgaagc gcctgacagt 12780ggcccaccag ctacaccaca gcggcaagta cggtggcgcg ccctggggta tgttcttggg 12840tccacaggag ctgcagcaca ttccaggtgc ggcggaggag gtggagcgac tggtcctgga 12900actggactgg tccaagcggt agaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 12960gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 13020cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 13080tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 13140gcggtttgcg tattgggcca aagacaaaag ggcgacattc aaccgattga gggagggaag 13200gtaaatattg acggaaatta ttcattaaag gtgaattatc accgtcaccg acttgagcca 13260
tttgggaatt agagccagca aaatcaccag tagcaccatt accattagca aggccggaaa 13320cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt agcgacagaa tcaagtttgc 13380ctttagcgtc agactgtagc gcgttttcat cggcattttc ggtcatagcc cccttattag 13440cgtttgccat cttttcataa tcaaaatcac cggaaccaga gccaccaccg gaaccgcctc 13500cctcagagcc gccaccctca gaaccgccac cctcagagcc accaccctca gagccgccac 13560cagaaccacc accagagccg ccgccagcat tgacaggagg cccgatctag taacatagat 13620gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat 13680taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat 13740tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca 13800agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg 13860atcatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg 13920tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgggcccg 13980atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt cgtaatgata 14040tcggcacctt cgaccgcctg ttccgcagag atcccgtggg cgaagaactc cagcatgaga 14100tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt 14160tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg 14220gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga 14280tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc 14340cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca 14400cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg 14460gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga 14520gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat 14580
cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt 14640cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg 14700atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca 14760atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc 14820ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg 14880acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg 14940catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag 15000cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccagatccgg 15060tgcagattat ttggattgag agtgaatatg agactctaat tggataccga ggggaattta 15120tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc 15180gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa 15240acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc 15300ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatca 15360gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt 15420aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg 15480tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttccccag atctggcgcc 15540ggccagcgag acgagcaaga ttggccgccg cccgaaacga tccgacagcg cgcccagcac 15600aggtgcgcag gcaaattgca ccaacgcata cagcgccagc agaatgccat agtgggcggt 15660gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc agcaccggca taatcaggcc 15720gatgccgaca gcgtcgagcg cgacagtgct cagaattacg atcaggggta tgttgggttt 15780cacgtctggc ctccggacca gcctccgctg gtccgattga acgcgcggat tctttatcac 15840tgataagttg gtggacatat tatgtttatc agtgataaag tgtcaagcat gacaaagttg 15900
cagccgaata cagtgatccg tgccgccctg gacctgttga acgaggtcgg cgtagacggt 15960ctgacgacac gcaaactggc ggaacggttg ggggttcagc agccggcgct ttactggcac 16020ttcaggaaca agcgggcgct gctcgacgca ctggccgaag ccatgctggc ggagaatcat 16080acgcattcgg tgccgagagc cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc 16140agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga 16200ccgggcgcac cgcagatgga aacggccgac gcgcagcttc gcttcctctg cgaggcgggt 16260ttttcggccg gggacgccgt caatgcgctg atgacaatca gctacttcac tgttggggcc 16320gtgcttgagg agcaggccgg cgacagcgat gccggcgagc gcggcggcac cgttgaacag 16380gctccgctct cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc cggtccggac 16440gcagcgttcg agcagggact cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt 16500gtcaggaacg ttgaaggacc gagaaagggt gacgattgat caggaccgct gccggagcgc 16560aacccactca ctacagcaga gccatgtaga caacatcccc tccccctttc caccgcgtca 16620gacgcccgta gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac 16680ggccgcgctc ggcctctctg gcggccttct ggcgctcttc cgcttcctcg ctcactgact 16740cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 16800ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 16860aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 16920acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 16980gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 17040ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgcttttc cgctgcataa 17100ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat 17160acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg 17220
ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat 17280tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc 17340gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca 17400cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg 17460gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc 17520gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg 17580ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc 17640acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg 17700atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg 17760ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga 17820gctggtgaag tacatcaccg acgagcaagg caagaccgag cgcctttgcg acgctca 17877<210>38<211>17238<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>38ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200
ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520
tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840
ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160
cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480
cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800
cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120
ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440
atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt ctaccgcttg 10800gaccagtcca gttccaggac cagtcgctcc acctcctccg ccgcacctgg aatgtgctgc 10860agctcctgtg gacccaagaa cataccccag ggcgcgccac cgtacttgcc gctgtggtgt 10920agctggtggg ccactgtcag gcgcttcatg tagggcaggc cagcgatggg cccggtggga 10980aagcgcctgt gcaccaggcc atcgtgtaca aacatatatg ccatgccgta tagcgtgatg 11040cccagccccg ctccaaagca ggccgccccc aggacgttgg gcagccagaa gccaaaggta 11100cacaggagca tggcgggcag tccattgatg attgcaaaca agtcgttggc ttcaaagggt 11160ccagtgcgag gtgtgtggtg gctcttgtgc agcagccagc ccagaggcga ctcatgccag 11220atggctttgt gtgcatagcg ggcatacatc tccatgccga gcgcgccacc aaccaccaag 11280aggagagtgc cagccacttc accccatggc actgcgccgc ccacggtcat gtgcatggca 11340aatctcaggt aggtggcgaa gatggcaatg cctgacacgc caattgatgc tgcaatggcg 11400gcagcctggt atgacagctg ctcccgtttg cgccgggcac gacgctctgc gatagcccgg 11460tcaagctgct ggagtgctac atcggcgctg tgctcatcgc ccgcgccggc agcctgcacg 11520gttcccagcg cctcctctgt ctgtggtgct gccactcgca gccgaactaa cgagcaccgc 11580tgagcatgca ggcagacttt gggccgcgtg atgtcgcggg ctagttcaac gcggcgggcc 11640ttgacgctga ttgactgcag cttcgacagc atagagataa aataaaaaga gaagaaaaga 11700aagtttgtac aatttctttt tgtttatata acatacacgc tatgtcaaca tttagaataa 11760
gggggaaaaa atcttccatc atattcgaat gcacaagatt atttctttgt tcgctctttt 11820tggtcgggtc atcgagattt agagtgtaat caaagatact gtcatctcga gagcgttgca 11880caggctgctg tttgccaaat tggatgtttg ccgaattagt aaaatacgca agcatttctt 11940acctttccgc tcccttttcc taattctccc aaagactaaa tgaggaaaga taaaggacaa 12000agaaaatgta aagacaaaga aattgaaaac gatataaact tgcagcacgt aagaccaaag 12060caaattggta actattcttg tgtacaaaca tgtataaaaa aaaacttttt tttgctcctg 12120gaggacaaaa tttcaaactc cttgaagaag attgcttgta tatctatcat atgcatatat 12180catatcgatg gaaaaagaaa gtcaggcatg tatttataaa aagaagaatg tgccatgctt 12240ccgaatttct tttcactttc ttttccttat ctattttaat ctcaagcttg gcgtaatcat 12300ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 12360ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 12420cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 12480tcggccaacg cgcggggaga ggcggtttgc gtattgggcc aaagacaaaa gggcgacatt 12540caaccgattg agggagggaa ggtaaatatt gacggaaatt attcattaaa ggtgaattat 12600caccgtcacc gacttgagcc atttgggaat tagagccagc aaaatcacca gtagcaccat 12660taccattagc aaggccggaa acgtcaccaa tgaaaccatc gatagcagca ccgtaatcag 12720tagcgacaga atcaagtttg cctttagcgt cagactgtag cgcgttttca tcggcatttt 12780cggtcatagc ccccttatta gcgtttgcca tcttttcata atcaaaatca ccggaaccag 12840agccaccacc ggaaccgcct ccctcagagc cgccaccctc agaaccgcca ccctcagagc 12900caccaccctc agagccgcca ccagaaccac caccagagcc gccgccagca ttgacaggag 12960gcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat 13020attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat 13080
ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga 13140aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc 13200caaatgtttg aacgatcggg gatcatccgg gtctgtggcg ggaactccac gaaaatatcc 13260gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg 13320ttgatgtgga cgccgggccc gatcatattg tcgctcagga tcgtggcgtt gtgcttgtcg 13380gccgttgctg tcgtaatgat atcggcacct tcgaccgcct gttccgcaga gatcccgtgg 13440gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa 13500acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc 13560aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt 13620caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga 13680ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta 13740tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc 13800cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcatcgc 13860cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct 13920cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga 13980tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc 14040gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat 14100cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga 14160gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct 14220gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg 14280ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc 14340cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca 14400
tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat gagactctaa 14460ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc 14520tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc 14580ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt 14640cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc 14700ttaattctcc gctcatgatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt 14760tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 14820atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 14880gggttcccca gatctggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg 14940atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag 15000cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg 15060cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac 15120gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg 15180aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa 15240gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg 15300aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag 15360cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa 15420gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca 15480tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg 15540cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt 15600cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc 15660agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag 15720
cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc 15780ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga 15840ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga 15900tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag acaacatccc 15960ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct 16020gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctctt 16080ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 16140ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 16200tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 16260tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 16320gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 16380ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 16440tggcgctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat 16500ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg 16560tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt 16620tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga 16680ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag 16740ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga acgaagagcg 16800attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct ggccgtcggc 16860cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct ggcccgcatc 16920aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga cgacccgcgc 16980acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga agagaagcag 17040
gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc atgacttttt 17100tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca tgcgctccat 17160caagaagagc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag gcaagaccga 17220gcgcctttgc gacgctca17238<210>39<211>17238<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>39ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300
caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620
gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940
cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcatt3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260
gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580
ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900
gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220
cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540
ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt agagataaaa 10800taaaaagaga agaaaagaaa gtttgtacaa tttctttttg tttatataac atacacgcta 10860
tgtcaacatt tagaataagg gggaaaaaat cttccatcat attcgaatgc acaagattat 10920ttctttgttc gctctttttg gtcgggtcat cgagatttag agtgtaatca aagatactgt 10980catctcgaga gcgttgcaca ggctgctgtt tgccaaattg gatgtttgcc gaattagtaa 11040aatacgcaag catttcttac ctttccgctc ccttttccta attctcccaa agactaaatg 11100aggaaagata aaggacaaag aaaatgtaaa gacaaagaaa ttgaaaacga tataaacttg 11160cagcacgtaa gaccaaagca aattggtaac tattcttgtg tacaaacatg tataaaaaaa 11220aacttttttt tgctcctgga ggacaaaatt tcaaactcct tgaagaagat tgcttgtata 11280tctatcatat gcatatatca tatcgatgga aaaagaaagt caggcatgta tttataaaaa 11340gaagaatgtg ccatgcttcc gaatttcttt tcactttctt ttccttatct attttaatct 11400catgctgtcg aagctgcagt caatcagcgt caaggcccgc cgcgttgaac tagcccgcga 11460catcacgcgg cccaaagtct gcctgcatgc tcagcggtgc tcgttagttc ggctgcgagt 11520ggcagcacca cagacagagg aggcgctggg aaccgtgcag gctgccggcg cgggcgatga 11580gcacagcgcc gatgtagcac tccagcagct tgaccgggct atcgcagagc gtcgtgcccg 11640gcgcaaacgg gagcagctgt cataccaggc tgccgccatt gcagcatcaa ttggcgtgtc 11700aggcattgcc atcttcgcca cctacctgag atttgccatg cacatgaccg tgggcggcgc 11760agtgccatgg ggtgaagtgg ctggcactct cctcttggtg gttggtggcg cgctcggcat 11820ggagatgtat gcccgctatg cacacaaagc catctggcat gagtcgcctc tgggctggct 11880gctgcacaag agccaccaca cacctcgcac tggacccttt gaagccaacg acttgtttgc 11940aatcatcaat ggactgcccg ccatgctcct gtgtaccttt ggcttctggc tgcccaacgt 12000cctgggggcg gcctgctttg gagcggggct gggcatcacg ctatacggca tggcatatat 12060gtttgtacac gatggcctgg tgcacaggcg ctttcccacc gggcccatcg ctggcctgcc 12120ctacatgaag cgcctgacag tggcccacca gctacaccac agcggcaagt acggtggcgc 12180
gccctggggt atgttcttgg gtccacagga gctgcagcac attccaggtg cggcggagga 12240ggtggagcga ctggtcctgg aactggactg gtccaagcgg tagaagcttg gcgtaatcat 12300ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 12360ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 12420cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 12480tcggccaacg cgcggggaga ggcggtttgc gtattgggcc aaagacaaaa gggcgacatt 12540caaccgattg agggagggaa ggtaaatatt gacggaaatt attcattaaa ggtgaattat 12600caccgtcacc gacttgagcc atttgggaat tagagccagc aaaatcacca gtagcaccat 12660taccattagc aaggccggaa acgtcaccaa tgaaaccatc gatagcagca ccgtaatcag 12720tagcgacaga atcaagtttg cctttagcgt cagactgtag cgcgttttca tcggcatttt 12780cggtcatagc ccccttatta gcgtttgcca tcttttcata atcaaaatca ccggaaccag 12840agccaccacc ggaaccgcct ccctcagagc cgccaccctc agaaccgcca ccctcagagc 12900caccaccctc agagccgcca ccagaaccac caccagagcc gccgccagca ttgacaggag 12960gcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat 13020attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat 13080ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga 13140aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc 13200caaatgtttg aacgatcggg gatcatccgg gtctgtggcg ggaactccac gaaaatatcc 13260gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg 13320ttgatgtgga cgccgggccc gatcatattg tcgctcagga tcgtggcgtt gtgcttgtcg 13380gccgttgctg tcgtaatgat atcggcacct tcgaccgcct gttccgcaga gatcccgtgg 13440gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa 13500
acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc 13560aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt 13620caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga 13680ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta 13740tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc 13800cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcatcgc 13860cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct 13920cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga 13980tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc 14040gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat 14100cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga 14160gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct 14220gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg 14280ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc 14340cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca 14400tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat gagactctaa 14460ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc 14520tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc 14580ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt 14640cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc 14700ttaattctcc gctcatgatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt 14760tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat 14820
atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 14880gggttcccca gatctggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg 14940atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag 15000cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg 15060cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac 15120gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg 15180aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa 15240gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg 15300aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag 15360cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa 15420gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca 15480tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg 15540cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt 15600cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc 15660agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag 15720cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc 15780ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga 15840ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga 15900tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag acaacatccc 15960ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct 16020gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctctt 16080ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 16140
ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 16200tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 16260tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 16320gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 16380ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 16440tggcgctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat 16500ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg 16560tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt 16620tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga 16680ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag 16740ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga acgaagagcg 16800attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct ggccgtcggc 16860cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct ggcccgcatc 16920aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga cgacccgcgc 16980acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga agagaagcag 17040gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc atgacttttt 17100tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca tgcgctccat 17160caagaagagc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag gcaagaccga 17220gcgcctttgc gacgctca17238<210>40<211>18449<212>DNA<213>人工序列
<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(3471)..(3471)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3679)..(3679)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3770)..(3770)<223>n為a，c，g，或t<400>40gatctttcga cactgaaata cgtcgagcct gctccgcttg gaagcggcga ggagcctcgt 60cctgtcacaa ctaccaacat ggagtacgat aagggccagt tccgccagct cattaagagc120cagttcatgg gcgttggcat gatggccgtc atgcatctgt acttcaagta caccaacgct180cttctgatcc agtcgatcat ccgctgaagg cgctttcgaa tctggttaag atccacgtct240tcgggaagcc agcgactggt gacctccagc gtccctttaa ggctgccaac agctttctca300gccagggcca gcccaagacc gacaaggcct ccctccagaa cgccgagaag aactggaggg360gtggtgtcaa ggaggagtaa gctccttatt gaagtcggag gacggagcgg tgtcaagagg420atattcttcg actctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca480tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc tttggctttc540aatattctca agtatctcga gtttgaactt attccctgtg aaccttttat tcaccaatga600gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc660ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca720
tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg780tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct840caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg900cacggctacg gaagacggag aagccacctt cagtggactc gagtaccatt taattctatt960tgtgtttgat cgagacctaa tacagcccct acaacgacca tcaaagtcgt atagctacca 1020gtgaggaagt ggactcaaat cgacttcagc aacatctcct ggataaactt taagcctaaa 1080ctatacagaa taagataggt ggagagctta taccgagctc ccaaatctgt ccagatcatg 1140gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct 1200ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa 1260aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga 1320gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca gctctggcgg 1380ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc cccgaagtgg 1440aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga atatggagct 1500tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta tagccgtcgg 1560cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc tggtaaaaga 1620ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg taagctccct 1680aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg 1740tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc 1800tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta 1860ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc 1920agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc 1980tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag 2040
taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca 2100gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc 2160accatgcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 2220gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 2280ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 2340tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 2400gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 2460gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 2520atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 2580ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 2640tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 2700atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 2760aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 2820ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 2880atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 2940ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 3000aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 3060gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 3120gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 3180tagagtagat gccgaccgcg ggatcgatcc acttaacgtt actgaaatca tcaaacagct 3240tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg 3300tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg 3360
atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata 3420cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg 3480gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga 3540tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat 3600atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat 3660gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact 3720ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa 3780gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac 3840gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca 3900cttgtttaga ggtaatcctt ctttctagct agaagtcctc gtgtactgtg taagcgccca 3960ctccacatct ccactcgacc tgcaggcatg caaagcttga gattaaaata gataaggaaa 4020agaaagtgaa aagaaattcg gaagcatggc acattcttct ttttataaat acatgcctga 4080ctttcttttt ccatcgatat gatatatgca tatgatagat atacaagcaa tcttcttcaa 4140ggagtttgaa attttgtcct ccaggagcaa aaaaaagttt ttttttatac atgtttgtac 4200acaagaatag ttaccaattt gctttggtct tacgtgctgc aagtttatat cgttttcaat 4260ttctttgtct ttacattttc tttgtccttt atctttcctc atttagtctt tgggagaatt 4320aggaaaaggg agcggaaagg taagaaatgc ttgcgtattt tactaattcg gcaaacatcc 4380aatttggcaa acagcagcct gtgcaacgct ctcgagatga cagtatcttt gattacactc 4440taaatctcga tgacccgacc aaaaagagcg aacaaagaaa taatcttgtg cattcgaata 4500tgatggaaga ttttttcccc cttattctaa atgttgacat agcgtgtatg ttatataaac 4560aaaaagaaat tgtacaaact ttcttttctt ctctttttat tttatctcta tgctgtcgaa 4620gctgcagtca atcagcgtca aggcccgccg cgttgaacta gcccgcgaca tcacgcggcc 4680
caaagtctgc ctgcatgctc agcggtgctc gttagttcgg ctgcgagtgg cagcaccaca 4740gacagaggag gcgctgggaa ccgtgcaggc tgccggcgcg ggcgatgagc acagcgccga 4800tgtagcactc cagcagcttg accgggctat cgcagagcgt cgtgcccggc gcaaacggga 4860gcagctgtca taccaggctg ccgccattgc agcatcaatt ggcgtgtcag gcattgccat 4920cttcgccacc tacctgagat ttgccatgca catgaccgtg ggcggcgcag tgccatgggg 4980tgaagtggct ggcactctcc tcttggtggt tggtggcgcg ctcggcatgg agatgtatgc 5040ccgctatgca cacaaagcca tctggcatga gtcgcctctg ggctggctgc tgcacaagag 5100ccaccacaca cctcgcactg gaccctttga agccaacgac ttgtttgcaa tcatcaatgg 5160actgcccgcc atgctcctgt gtacctttgg cttctggctg cccaacgtcc tgggggcggc 5220ctgctttgga gcggggctgg gcatcacgct atacggcatg gcatatatgt ttgtacacga 5280tggcctggtg cacaggcgct ttcccaccgg gcccatcgct ggcctgccct acatgaagcg 5340cctgacagtg gcccaccagc tacaccacag cggcaagtac ggtggcgcgc cctggggtat 5400gttcttgggt ccacaggagc tgcagcacat tccaggtgcg gcggaggagg tggagcgact 5460ggtcctggaa ctggactggt ccaagcggta gattgtgact gatagcgaga ctctgggtcg 5520atgttatctg cctcaacaat ggcttagaaa agaagaaaca gaacaaatac agcaaggcaa 5580cgcccgtagc ctaggtgatc aaagactgtt gggcttgtct ctgaagcttg taggaaaggc 5640agacgctatc atggtgagag ctaagaaggg cattgacaag ttgccggcaa actgtcaagg 5700cggtgtacga gctgcttgcc aagtatatgc tgcaattgga tctgtactca agcagcagaa 5760gacaacatat cctacaagag ctcatctaaa aggaagcgaa cgtgccaaga ttgctctgtt 5820gagtgtatac aacctctatc aatctgaaga caagcctgtg gctctccgtc aagctagaaa 5880gattaagagt ttttttgttg attagtgaat ttttgtttta tttatgtctg atagttcaat 5940aaagagacaa cacatacaat ataaaatcat tgtctttaaa tgttaattta gtagagtgta 6000
aagcctgcat tttttttgta cgcataaaca atgaattcac cccgcttctg gtttttaaat 6060aattatgtca aactagggaa aattcttttt tttctcttcg ttcttttttt ggcttgttgt 6120ggagtcacag gcttgtcttc agattgatag aggttgtata cactcaacag agcaatcttg 6180gcacgttcgc ttccttttag atgagctctt gtaggatatg ttgtcttctg ctgcttgagt 6240acagatccaa ttgcagcata tacttggcaa gcagctcgta caccgccttg acagtttgcc 6300ggcaacttgt caatgccctt cttagctctc accatgatag cgtctgcctt tcctacaagc 6360ttcagagaca agcccaacag tctttgatca cctaggctac gggcgttgcc ttgctgtatt 6420tgttctgttt cttcttttct aagccattgt tgaggcagat aacatcgacc caacatcctc 6480gagccatact acagcataaa aggatacgtt ttctttaaca gaaatttacc cttttgttat 6540cagcacatac aaaaaaaaag aaatttaaga tgagtaggac ttccattctc tcaaaaattt 6600tattcaatcc ataaatgaat tatttttgga caaaaaagaa agattatgcc tgattttctc 6660tatttttttt ttttttacaa ctccaccaat actttctagc ccagcttggc gtaatcatgg 6720tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 6780ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 6840ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 6900ggccaacgcg cggggagagg cggtttgcgt attgggccaa agacaaaagg gcgacattca 6960accgattgag ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca 7020ccgtcaccga cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta 7080ccattagcaa ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta 7140gcgacagaat caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg 7200gtcatagccc ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag 7260ccaccaccgg aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca 7320
ccaccctcag agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggc 7380ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat 7440tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct 7500cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa 7560ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca 7620aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg aactccacga aaatatccga 7680acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt 7740gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc 7800cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc 7860gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac 7920gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag 7980gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca 8040agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg 8100aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg 8160tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca 8220ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg 8280tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct 8340tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg 8400cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc 8460attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc 8520tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc 8580acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc 8640
agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 8700gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg 8760aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg 8820cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt 8880ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta 8940gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt 9000agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca 9060gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt 9120aattctccgc tcatgatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg 9180acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat 9240ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg 9300gttccccaga tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat 9360ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca 9420gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca 9480gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga 9540tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa 9600cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt 9660gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa 9720cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca 9780gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc 9840catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt 9900tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg 9960
catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg 10020cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag 10080ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg 10140cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt 10200cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt 10260ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc 10320aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct 10380ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc 10440cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc 10500gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 10560cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 10620tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 10680cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 10740aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 10800cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 10860gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc 10920atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg 10980tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc 11040cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg 11100ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc 11160aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat 11220tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca 11280
gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa 11340tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac 11400ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga 11460cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta 11520gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca 11580agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc 11640gcctttgcga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc 11700cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt 11760tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact 11820tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg 11880gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc 11940ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc 12000gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga 12060tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc 12120aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca 12180atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg 12240aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc 12300ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt 12360ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc 12420cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag 12480tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga 12540cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc 12600
cgtgctcgtg ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt 12660ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat 12720ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat 12780attgacaata ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga 12840tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca 12900taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt 12960ctatcataat tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc 13020gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg 13080tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct 13140gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca 13200tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg 13260ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca 13320gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat 13380gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga 13440cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca 13500ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac 13560tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt 13620ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat agacacagaa 13680gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc 13740cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac 13800aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg 13860gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa 13920
ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat 13980accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag 14040aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg 14100tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc 14160tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc 14220tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg 14280agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag 14340acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg 14400aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga 14460cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct 14520ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca 14580agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt 14640atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt 14700atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag 14760caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc 14820aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac 14880gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg 14940gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc 15000ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa 15060gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc 15120atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc 15180aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc 15240
gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc 15300gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa 15360caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa 15420atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac 15480gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg 15540caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag 15600ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc 15660cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg 15720atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg 15780atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc 15840cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc 15900gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg 15960tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc 16020aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc 16080gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg 16140gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg 16200ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact 16260tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag 16320gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc 16380aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg 16440tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg 16500tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg 16560
acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc 16620gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga 16680tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac 16740ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg 16800tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc 16860taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg 16920cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg 16980cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc 17040ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag 17100ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt 17160ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac 17220tcgaacctac agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc 17280cggggatgca tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag 17340caatggatag gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc 17400ttcctcagcg gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca 17460gcctgtcacg gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg 17520agatgatatt tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct 17580ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc 17640ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact 17700gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg 17760ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac 17820acattgcgga cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa 17880
cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt 17940ttgccccagc aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat 18000aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 18060ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 18120ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 18180aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 18240gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg 18300ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 18360tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 18420ggccagtgaa ttcgagctcg gtacccggg18449<210>41<211>18449<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(3471)..(3471)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3679)..(3679)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3770)..(3770)
<223>n為a，c，g，或t<400>41gatctttcga cactgaaata cgtcgagcct gctccgcttg gaagcggcga ggagcctcgt 60cctgtcacaa ctaccaacat ggagtacgat aagggccagt tccgccagct cattaagagc120cagttcatgg gcgttggcat gatggccgtc atgcatctgt acttcaagta caccaacgct180cttctgatcc agtcgatcat ccgctgaagg cgctttcgaa tctggttaag atccacgtct240tcgggaagcc agcgactggt gacctccagc gtccctttaa ggctgccaac agctttctca300gccagggcca gcccaagacc gacaaggcct ccctccagaa cgccgagaag aactggaggg360gtggtgtcaa ggaggagtaa gctccttatt gaagtcggag gacggagcgg tgtcaagagg420atattcttcg actctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca480tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc tttggctttc540aatattctca agtatctcga gtttgaactt attccctgtg aaccttttat tcaccaatga600gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc660ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca720tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg780tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct840caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg900cacggctacg gaagacggag aagccacctt cagtggactc gagtaccatt taattctatt960tgtgtttgat cgagacctaa tacagcccct acaacgacca tcaaagtcgt atagctacca 1020gtgaggaagt ggactcaaat cgacttcagc aacatctcct ggataaactt taagcctaaa 1080ctatacagaa taagataggt ggagagctta taccgagctc ccaaatctgt ccagatcatg 1140gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct 1200ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa 1260
aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga 1320gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca gctctggcgg 1380ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc cccgaagtgg 1440aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga atatggagct 1500tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta tagccgtcgg 1560cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc tggtaaaaga 1620ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg taagctccct 1680aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg 1740tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc 1800tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta 1860ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc 1920agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc 1980tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag 2040taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca 2100gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc 2160accatgcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 2220gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 2280ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 2340tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 2400gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 2460gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 2520atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 2580
ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 2640tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 2700atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 2760aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 2820ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 2880atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 2940ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 3000aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 3060gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 3120gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 3180tagagtagat gccgaccgcg ggatcgatcc acttaacgtt actgaaatca tcaaacagct 3240tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg 3300tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg 3360atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata 3420cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg 3480gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga 3540tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat 3600atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat 3660gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact 3720ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa 3780gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac 3840gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca 3900
cttgtttaga ggtaatcctt ctttctagct agaagtcctc gtgtactgtg taagcgccca 3960ctccacatct ccactcgacc tgcaggcatg caaagcttga gattaaaata gataaggaaa 4020agaaagtgaa aagaaattcg gaagcatggc acattcttct ttttataaat acatgcctga 4080ctttcttttt ccatcgatat gatatatgca tatgatagat atacaagcaa tcttcttcaa 4140ggagtttgaa attttgtcct ccaggagcaa aaaaaagttt ttttttatac atgtttgtac 4200acaagaatag ttaccaattt gctttggtct tacgtgctgc aagtttatat cgttttcaat 4260ttctttgtct ttacattttc tttgtccttt atctttcctc atttagtctt tgggagaatt 4320aggaaaaggg agcggaaagg taagaaatgc ttgcgtattt tactaattcg gcaaacatcc 4380aatttggcaa acagcagcct gtgcaacgct ctcgagatga cagtatcttt gattacactc 4440taaatctcga tgacccgacc aaaaagagcg aacaaagaaa taatcttgtg cattcgaata 4500tgatggaaga ttttttcccc cttattctaa atgttgacat agcgtgtatg ttatataaac 4560aaaaagaaat tgtacaaact ttcttttctt ctctttttat tttatctcta tgctgtcgaa 4620gctgcagtca atcagcgtca aggcccgccg cgttgaacta gcccgcgaca tcacgcggcc 4680caaagtctgc ctgcatgctc agcggtgctc gttagttcgg ctgcgagtgg cagcaccaca 4740gacagaggag gcgctgggaa ccgtgcaggc tgccggcgcg ggcgatgagc acagcgccga 4800tgtagcactc cagcagcttg accgggctat cgcagagcgt cgtgcccggc gcaaacggga 4860gcagctgtca taccaggctg ccgccattgc agcatcaatt ggcgtgtcag gcattgccat 4920cttcgccacc tacctgagat ttgccatgca catgaccgtg ggcggcgcag tgccatgggg 4980tgaagtggct ggcactctcc tcttggtggt tggtggcgcg ctcggcatgg agatgtatgc 5040ccgctatgca cacaaagcca tctggcatga gtcgcctctg ggctggctgc tgcacaagag 5100ccaccacaca cctcgcactg gaccctttga agccaacgac ttgtttgcaa tcatcaatgg 5160actgcccgcc atgctcctgt gtacctttgg cttctggctg cccaacgtcc tgggggcggc 5220
ctgctttgga gcggggctgg gcatcacgct atacggcatg gcatatatgt ttgtacacga 5280tggcctggtg cacaggcgct ttcccaccgg gcccatcgct ggcctgccct acatgaagcg 5340cctgacagtg gcccaccagc tacaccacag cggcaagtac ggtggcgcgc cctggggtat 5400gttcttgggt ccacaggagc tgcagcacat tccaggtgcg gcggaggagg tggagcgact 5460ggtcctggaa ctggactggt ccaagcgggc gattgtgact gatagcgaga ctctgggtcg 5520atgttatctg cctcaacaat ggcttagaaa agaagaaaca gaacaaatac agcaaggcaa 5580cgcccgtagc ctaggtgatc aaagactgtt gggcttgtct ctgaagcttg taggaaaggc 5640agacgctatc atggtgagag ctaagaaggg cattgacaag ttgccggcaa actgtcaagg 5700cggtgtacga gctgcttgcc aagtatatgc tgcaattgga tctgtactca agcagcagaa 5760gacaacatat cctacaagag ctcatctaaa aggaagcgaa cgtgccaaga ttgctctgtt 5820gagtgtatac aacctctatc aatctgaaga caagcctgtg gctctccgtc aagctagaaa 5880gattaagagt ttttttgttg attagtgaat ttttgtttta tttatgtctg atagttcaat 5940aaagagacaa cacatacaat ataaaatcat tgtctttaaa tgttaattta gtagagtgta 6000aagcctgcat tttttttgta cgcataaaca atgaattcac cccgcttctg gtttttaaat 6060aattatgtca aactagggaa aattcttttt tttctcttcg ttcttttttt ggcttgttgt 6120ggagtcacag gcttgtcttc agattgatag aggttgtata cactcaacag agcaatcttg 6180gcacgttcgc ttccttttag atgagctctt gtaggatatg ttgtcttctg ctgcttgagt 6240acagatccaa ttgcagcata tacttggcaa gcagctcgta caccgccttg acagtttgcc 6300ggcaacttgt caatgccctt cttagctctc accatgatag cgtctgcctt tcctacaagc 6360ttcagagaca agcccaacag tctttgatca cctaggctac gggcgttgcc ttgctgtatt 6420tgttctgttt cttcttttct aagccattgt tgaggcagat aacatcgacc caacatcctc 6480gagccatact acagcataaa aggatacgtt ttctttaaca gaaatttacc cttttgttat 6540
cagcacatac aaaaaaaaag aaatttaaga tgagtaggac ttccattctc tcaaaaattt 6600tattcaatcc ataaatgaat tatttttgga caaaaaagaa agattatgcc tgattttctc 6660tatttttttt ttttttacaa ctccaccaat actttctagc ccagcttggc gtaatcatgg 6720tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 6780ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 6840ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 6900ggccaacgcg cggggagagg cggtttgcgt attgggccaa agacaaaagg gcgacattca 6960accgattgag ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca 7020ccgtcaccga cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta 7080ccattagcaa ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta 7140gcgacagaat caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg 7200gtcatagccc ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag 7260ccaccaccgg aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca 7320ccaccctcag agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggc 7380ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat 7440tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct 7500cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa 7560ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca 7620aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg aactccacga aaatatccga 7680acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt 7740gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc 7800cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc 7860
gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac 7920gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag 7980gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca 8040agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg 8100aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg 8160tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca 8220ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg 8280tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct 8340tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg 8400cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc 8460attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc 8520tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc 8580acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc 8640agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct 8700gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg 8760aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg 8820cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt 8880ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta 8940gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt 9000agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca 9060gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt 9120aattctccgc tcatgatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg 9180
acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat 9240ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg 9300gttccccaga tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat 9360ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca 9420gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca 9480gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga 9540tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa 9600cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt 9660gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa 9720cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca 9780gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc 9840catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt 9900tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg 9960catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg 10020cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag 10080ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg 10140cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt 10200cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt 10260ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc 10320aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct 10380ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc 10440cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc 10500
gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 10560cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 10620tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 10680cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 10740aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 10800cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 10860gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc 10920atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg 10980tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc 11040cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg 11100ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc 11160aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat 11220tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca 11280gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa 11340tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac 11400ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga 11460cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta 11520gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca 11580agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc 11640gcctttgcga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc 11700cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt 11760tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact 11820
tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg 11880gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc 11940ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc 12000gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga 12060tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc 12120aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca 12180atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg 12240aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc 12300ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt 12360ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc 12420cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag 12480tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga 12540cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc 12600cgtgctcgtg ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt 12660ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat 12720ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat 12780attgacaata ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga 12840tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca 12900taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt 12960ctatcataat tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc 13020gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg 13080tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct 13140
gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca 13200tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg 13260ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca 13320gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat 13380gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga 13440cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca 13500ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac 13560tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt 13620ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat agacacagaa 13680gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc 13740cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac 13800aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg 13860gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa 13920ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat 13980accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag 14040aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg 14100tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc 14160tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc 14220tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg 14280agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag 14340acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg 14400aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga 14460
cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct 14520ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca 14580agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt 14640atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt 14700atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag 14760caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc 14820aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac 14880gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg 14940gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc 15000ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa 15060gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc 15120atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc 15180aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc 15240gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc 15300gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa 15360caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa 15420atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac 15480gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg 15540caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag 15600ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc 15660cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg 15720atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg 15780
atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc 15840cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc 15900gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg 15960tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc 16020aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc 16080gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg 16140gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg 16200ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact 16260tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag 16320gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc 16380aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg 16440tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg 16500tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg 16560acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc 16620gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga 16680tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac 16740ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg 16800tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc 16860taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg 16920cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg 16980cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc 17040ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag 17100
ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt 17160ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac 17220tcgaacctac agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc 17280cggggatgca tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag 17340caatggatag gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc 17400ttcctcagcg gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca 17460gcctgtcacg gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg 17520agatgatatt tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct 17580ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc 17640ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact 17700gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg 17760ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac 17820acattgcgga cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa 17880cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt 17940ttgccccagc aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat 18000aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 18060ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 18120ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 18180aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 18240gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg 18300ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 18360tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 18420
ggccagtgaa ttcgagctcg gtacccggg18449<210>42<211>17593<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>42ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480
ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800
ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120
tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440
cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760
tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080
aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400
atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720
aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt ttttcgagtt 10800tttttttttt ttctttgtga aggatttatt gttattggta tccatttttt attggaagac 10860aagataagtt aatattgatt ttgcttaaag attaaaagga aatcagaaaa cgacaataaa 10920aaatgtaacg gacaaactat ggtgtcgatt ataagtctaa atccttaaaa aatgacaacg 10980agttgctttc ctctgaaaac aattcttttg tctttgcaag aaaggtttct tttttgtttg 11040
cttgcattac ttaaacatca aatcaaatga aaggaataaa gcagatttga gggcgaataa 11100ggattttctg gtcaacaaga tgtgagtgac acctaaggaa ctaaatgcca ttcatttgtt 11160ttaaaacgac atcaaagatt gatgatcaac aggattgaga gagagaaaaa gaactcgtgt 11220catttatttc tgttgactga aattttatat ttagaaaaaa tgtcaaatct atagctttag 11280ctatattaca taacatttga aataataata ataaaaaaag acacattaga gacacttttc 11340aaactctaaa taactgtcta taaacacaaa gaaaacaaag acctctataa caacttatta 11400gatttttctc gtacttttgt ctaaagatga tgtattcttg ttatcccaca cttctttcat 11460ttgttcttga tgctactaaa tatacaaaat ttcttttttg caagagatat tattccaaaa 11520attttcaaaa agaaattttt ttcacaatag cagttgatcg tgtaacccaa agaggttctt 11580tgttattttg cacttccgct ttgcggtgat gcatattcaa agtaatatat ggaataaaca 11640acgtgtttaa gcatgaaaga aaggaaacaa aggccgcttt gaacaaatgc ataatatttc 11700agacaaaaat gatctaaagc aagcagtaaa tcaaacaaga aacattgctg attcgcgtta 11760gaaaacgata aaagtctaat aagccactaa gtatacttca atgaactttt tgtatgctta 11820tggtccaatc agaccaataa tttgtgacca ttcctgaggt ggctttggtg atgcggaaac 11880agaaaaaaat tttctcacca atcgatttaa aaaacaattt ctgctttgaa ccaaaacttt 11940ttttttctct ttaatcatta actttatcaa gtatgtacct accctcaaag tcctcactca 12000agcacaatta tgctaacatt gttccacctt ctctttagaa atgttgtgga tttggaatgc 12060cctgatcgtt ttcgttaccg tgattggcat ggaagtgatt gctgcactgg cacacaaata 12120catcatgcac ggctggggtt ggggatggca tctttcacat catgaaccgc gtaaaggtgc 12180gtttgaagtt aacgatcttt atgccgtggt ttttgctgca ttatcgatcc tgctgattta 12240tctgggcagt acaggaatgt ggccgctcca gtggattggc gcaggtatga cggcgtatgg 12300attactctat tttatggtgc acgacgggct ggtgcatcaa cgttggccat tccgctatat 12360
tccacgcaag ggctacctca aacggttgta tatggcgcac cgtatgcatc acgccgtcag 12420gggcaaagaa ggttgtgttt cttttggctt cctctatgcg ccgcccctgt caaaacttca 12480ggcgacgctc cgggaaagac atggcgctag agcgggcgct gccagagatg cgcagggcgg 12540ggaggatgag cccgcatccg ggaagtaagg gcctgaccag aggcggccag cagcagcgtt 12600aatttttcgg gcgtggtcgt tgactgccgc tgatcccaaa gcttggcgta atcatggtca 12660tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 12720agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 12780cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 12840caacgcgcgg ggagaggcgg tttgcgtatt gggccaaaga caaaagggcg acattcaacc 12900gattgaggga gggaaggtaa atattgacgg aaattattca ttaaaggtga attatcaccg 12960tcaccgactt gagccatttg ggaattagag ccagcaaaat caccagtagc accattacca 13020ttagcaaggc cggaaacgtc accaatgaaa ccatcgatag cagcaccgta atcagtagcg 13080acagaatcaa gtttgccttt agcgtcagac tgtagcgcgt tttcatcggc attttcggtc 13140atagccccct tattagcgtt tgccatcttt tcataatcaa aatcaccgga accagagcca 13200ccaccggaac cgcctccctc agagccgcca ccctcagaac cgccaccctc agagccacca 13260ccctcagagc cgccaccaga accaccacca gagccgccgc cagcattgac aggaggcccg 13320atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt 13380gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat 13440aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta 13500tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat 13560gtttgaacga tcggggatca tccgggtctg tggcgggaac tccacgaaaa tatccgaacg 13620cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat 13680
gtggacgccg ggcccgatca tattgtcgct caggatcgtg gcgttgtgct tgtcggccgt 13740tgctgtcgta atgatatcgg caccttcgac cgcctgttcc gcagagatcc cgtgggcgaa 13800gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat 13860tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt 13920gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 13980aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 14040cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 14100tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 14160tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg 14220ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 14280tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 14340tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 14400gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 14460cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 14520gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt 14580tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 14640agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 14700agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga 14760aacgatccag atccggtgca gattatttgg attgagagtg aatatgagac tctaattgga 14820taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct 14880gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc 14940tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt 15000
ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat 15060tctccgctca tgatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca 15120ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta 15180aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt 15240ccccagatct ggcgccggcc agcgagacga gcaagattgg ccgccgcccg aaacgatccg 15300acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc gccagcagaa 15360tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg cccggcagca 15420ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga attacgatca 15480ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc gattgaacgc 15540gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg ataaagtgtc 15600aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc tgttgaacga 15660ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg ttcagcagcc 15720ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat 15780gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc gctcatttct 15840gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg gcgcgcgcat 15900ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt 15960cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga caatcagcta 16020cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg gcgagcgcgg 16080cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag acgccttcga 16140cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg atggattggc 16200gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg attgatcagg 16260accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc 16320
cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct 16380agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg ctcttccgct 16440tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 16500tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 16560gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 16620aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 16680ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 16740gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 16800cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc 16860ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat 16920ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt 16980cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg 17040gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac 17100caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga 17160ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg 17220ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg 17280cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc 17340gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga 17400gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc 17460gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga 17520agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag accgagcgcc 17580tttgcgacgc tca 17593
<210>43<211>16954<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>43ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540
tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860
tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180
ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500
tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820
cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140
aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460
gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780
cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt gagattaaaa 10800tagataagga aaagaaagtg aaaagaaatt cggaagcatg gcacattctt ctttttataa 10860atacatgcct gactttcttt ttccatcgat atgatatatg catatgatag atatacaagc 10920aatcttcttc aaggagtttg aaattttgtc ctccaggagc aaaaaaaagt ttttttttat 10980acatgtttgt acacaagaat agttaccaat ttgctttggt cttacgtgct gcaagtttat 11040atcgttttca atttctttgt ctttacattt tctttgtcct ttatctttcc tcatttagtc 11100
tttgggagaa ttaggaaaag ggagcggaaa ggtaagaaat gcttgcgtat tttactaatt 11160cggcaaacat ccaatttggc aaacagcagc ctgtgcaacg ctctcgagat gacagtatct 11220ttgattacac tctaaatctc gatgacccga ccaaaaagag cgaacaaaga aataatcttg 11280tgcattcgaa tatgatggaa gattttttcc cccttattct aaatgttgac atagcgtgta 11340tgttatataa acaaaaagaa attgtacaaa ctttcttttc ttctcttttt attttatctc 11400tatgttgtgg atttggaatg ccctgatcgt tttcgttacc gtgattggca tggaagtgat 11460tgctgcactg gcacacaaat acatcatgca cggctggggt tggggatggc atctttcaca 11520tcatgaaccg cgtaaaggtg cgtttgaagt taacgatctt tatgccgtgg tttttgctgc 11580attatcgatc ctgctgattt atctgggcag tacaggaatg tggccgctcc agtggattgg 11640cgcaggtatg acggcgtatg gattactcta ttttatggtg cacgacgggc tggtgcatca 11700acgttggcca ttccgctata ttccacgcaa gggctacctc aaacggttgt atatggcgca 11760ccgtatgcat cacgccgtca ggggcaaaga aggttgtgtt tcttttggct tcctctatgc 11820gccgcccctg tcaaaacttc aggcgacgct ccgggaaaga catggcgcta gagcgggcgc 11880tgccagagat gcgcagggcg gggaggatga gcccgcatcc gggaagtaag ggcctgacca 11940gaggcggcca gcagcagcgt taatttttcg ggcgtggtcg ttgactgccg ctgatcccaa 12000agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 12060ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 12120taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 12180cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggccaaag 12240acaaaagggc gacattcaac cgattgaggg agggaaggta aatattgacg gaaattattc 12300attaaaggtg aattatcacc gtcaccgact tgagccattt gggaattaga gccagcaaaa 12360tcaccagtag caccattacc attagcaagg ccggaaacgt caccaatgaa accatcgata 12420
gcagcaccgt aatcagtagc gacagaatca agtttgcctt tagcgtcaga ctgtagcgcg 12480ttttcatcgg cattttcggt catagccccc ttattagcgt ttgccatctt ttcataatca 12540aaatcaccgg aaccagagcc accaccggaa ccgcctccct cagagccgcc accctcagaa 12600ccgccaccct cagagccacc accctcagag ccgccaccag aaccaccacc agagccgccg 12660ccagcattga caggaggccc gatctagtaa catagatgac accgcgcgcg ataatttatc 12720ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct 12780aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt 12840aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct 12900taagaaactt tattgccaaa tgtttgaacg atcggggatc atccgggtct gtggcgggaa 12960ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca 13020gtcgccgccg acgccgttga tgtggacgcc gggcccgatc atattgtcgc tcaggatcgt 13080ggcgttgtgc ttgtcggccg ttgctgtcgt aatgatatcg gcaccttcga ccgcctgttc 13140cgcagagatc ccgtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca 13200gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc 13260gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc 13320gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg 13380ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca 13440cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg 13500aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc 13560acgacgagat catcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc 13620gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga 13680gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca 13740
agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg 13800tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct 13860tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc 13920cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga 13980accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt 14040tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat 14100ccatcttgtt caatcatgcg aaacgatcca gatccggtgc agattatttg gattgagagt 14160gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt 14220gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg 14280tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc 14340aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg 14400tcataacgtg actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt 14460ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 14520attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 14580gtgcatgcca accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg 14640gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca 14700acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat 14760cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga 14820cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc 14880tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat 14940gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc 15000cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga 15060
acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct 15120cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga 15180cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc 15240ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac 15300ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa 15360tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga 15420cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg 15480ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc 15540ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag 15600aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc 15660atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg 15720ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg 15780gccttctggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 15840cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 15900aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 15960gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 16020tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 16080agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 16140ctcccttcgg gaagcgtggc gcttttccgc tgcataaccc tgcttcgggg tcattatagc 16200gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg 16260tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc 16320acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg 16380
gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat 16440ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc 16500agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta 16560cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg 16620cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct 16680caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa 16740gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc 16800agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg 16860tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg 16920agcaaggcaa gaccgagcgc ctttgcgacg ctca 16954<210>44<211>16954<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)
<223>n為a，c，g，或t<400>44ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260
ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580
tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900
tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220
attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540
ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860
ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180
gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500
tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt agagataaaa 10800taaaaagaga agaaaagaaa gtttgtacaa tttctttttg tttatataac atacacgcta 10860tgtcaacatt tagaataagg gggaaaaaat cttccatcat attcgaatgc acaagattat 10920ttctttgttc gctctttttg gtcgggtcat cgagatttag agtgtaatca aagatactgt 10980catctcgaga gcgttgcaca ggctgctgtt tgccaaattg gatgtttgcc gaattagtaa 11040aatacgcaag catttcttac ctttccgctc ccttttccta attctcccaa agactaaatg 11100aggaaagata aaggacaaag aaaatgtaaa gacaaagaaa ttgaaaacga tataaacttg 11160cagcacgtaa gaccaaagca aattggtaac tattcttgtg tacaaacatg tataaaaaaa 11220aacttttttt tgctcctgga ggacaaaatt tcaaactcct tgaagaagat tgcttgtata 11280tctatcatat gcatatatca tatcgatgga aaaagaaagt caggcatgta tttataaaaa 11340gaagaatgtg ccatgcttcc gaatttcttt tcactttctt ttccttatct attttaatct 11400catgttgtgg atttggaatg ccctgatcgt tttcgttacc gtgattggca tggaagtgat 11460tgctgcactg gcacacaaat acatcatgca cggctggggt tggggatggc atctttcaca 11520tcatgaaccg cgtaaaggtg cgtttgaagt taacgatctt tatgccgtgg tttttgctgc 11580attatcgatc ctgctgattt atctgggcag tacaggaatg tggccgctcc agtggattgg 11640cgcaggtatg acggcgtatg gattactcta ttttatggtg cacgacgggc tggtgcatca 11700acgttggcca ttccgctata ttccacgcaa gggctacctc aaacggttgt atatggcgca 11760ccgtatgcat cacgccgtca ggggcaaaga aggttgtgtt tcttttggct tcctctatgc 11820
gccgcccctg tcaaaacttc aggcgacgct ccgggaaaga catggcgcta gagcgggcgc 11880tgccagagat gcgcagggcg gggaggatga gcccgcatcc gggaagtaag ggcctgacca 11940gaggcggcca gcagcagcgt taatttttcg ggcgtggtcg ttgactgccg ctgatcccaa 12000agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 12060ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 12120taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 12180cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggccaaag 12240acaaaagggc gacattcaac cgattgaggg agggaaggta aatattgacg gaaattattc 12300attaaaggtg aattatcacc gtcaccgact tgagccattt gggaattaga gccagcaaaa 12360tcaccagtag caccattacc attagcaagg ccggaaacgt caccaatgaa accatcgata 12420gcagcaccgt aatcagtagc gacagaatca agtttgcctt tagcgtcaga ctgtagcgcg 12480ttttcatcgg cattttcggt catagccccc ttattagcgt ttgccatctt ttcataatca 12540aaatcaccgg aaccagagcc accaccggaa ccgcctccct cagagccgcc accctcagaa 12600ccgccaccct cagagccacc accctcagag ccgccaccag aaccaccacc agagccgccg 12660ccagcattga caggaggccc gatctagtaa catagatgac accgcgcgcg ataatttatc 12720ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct 12780aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt 12840aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct 12900taagaaactt tattgccaaa tgtttgaacg atcggggatc atccgggtct gtggcgggaa 12960ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca 13020gtcgccgccg acgccgttga tgtggacgcc gggcccgatc atattgtcgc tcaggatcgt 13080ggcgttgtgc ttgtcggccg ttgctgtcgt aatgatatcg gcaccttcga ccgcctgttc 13140
cgcagagatc ccgtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca 13200gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc 13260gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc 13320gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg 13380ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca 13440cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg 13500aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc 13560acgacgagat catcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc 13620gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga 13680gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca 13740agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg 13800tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct 13860tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc 13920cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga 13980accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt 14040tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat 14100ccatcttgtt caatcatgcg aaacgatcca gatccggtgc agattatttg gattgagagt 14160gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt 14220gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg 14280tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc 14340aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg 14400tcataacgtg actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt 14460
ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 14520attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 14580gtgcatgcca accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg 14640gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca 14700acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat 14760cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga 14820cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc 14880tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat 14940gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc 15000cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga 15060acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct 15120cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga 15180cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc 15240ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac 15300ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa 15360tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga 15420cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg 15480ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc 15540ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag 15600aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc 15660atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg 15720ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg 15780
gccttctggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 15840cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 15900aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 15960gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 16020tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 16080agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 16140ctcccttcgg gaagcgtggc gcttttccgc tgcataaccc tgcttcgggg tcattatagc 16200gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg 16260tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc 16320acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg 16380gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat 16440ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc 16500agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta 16560cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg 16620cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct 16680caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa 16740gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc 16800agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg 16860tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg 16920agcaaggcaa gaccgagcgc ctttgcgacg ctca 16954<210>45<211>19491
<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(18970)..(18970)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(19178)..(19178)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(19269)..(19269)<223>n為a，c，g，或t<400>45agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt 60gacggccagt gaattcgagc tcggtacccg gggatctttc gacactgaaa tacgtcgagc120ctgctccgct tggaagcggc gaggagcctc gtcctgtcac aactaccaac atggagtacg180ataagggcca gttccgccag ctcattaaga gccagttcat gggcgttggc atgatggccg240tcatgcatct gtacttcaag tacaccaacg ctcttctgat ccagtcgatc atccgctgaa300ggcgctttcg aatctggtta agatccacgt cttcgggaag ccagcgactg gtgacctcca360gcgtcccttt aaggctgcca acagctttct cagccagggc cagcccaaga ccgacaaggc420ctccctccag aacgccgaga agaactggag gggtggtgtc aaggaggagt aagctcctta480ttgaagtcgg aggacggagc ggtgtcaaga ggatattctt cgactctgta ttatagataa540gatgatgagg aattggaggt agcatagctt catttggatt tgctttccag gctgagactc600tagcttggag catagagggt cctttggctt tcaatattct caagtatctc gagtttgaac660
ttattccctg tgaacctttt attcaccaat gagcattgga atgaacatga atctgaggac720tgcaatcgcc atgaggtttt cgaaatacat ccggatgtcg aaggcttggg gcacctgcgt780tggttgaatt tagaacgtgg cactattgat catccgatag ctctgcaaag ggcgttgcac840aatgcaagtc aaacgttgct agcagttcca ggtggaatgt tatgatgagc attgtattaa900atcaggagat atagcatgat ctctagttag ctcaccacaa aagtcagacg gcgtaaccaa960aagtcacaca acacaagctg taaggatttc ggcacggcta cggaagacgg agaagccacc 1020ttcagtggac tcgagtacca tttaattcta tttgtgtttg atcgagacct aatacagccc 1080ctacaacgac catcaaagtc gtatagctac cagtgaggaa gtggactcaa atcgacttca 1140gcaacatctc ctggataaac tttaagccta aactatacag aataagatag gtggagagct 1200tataccgagc tcccaaatct gtccagatca tggttgaccg gtgcctggat cttcctatag 1260aatcatcctt attcgttgac ctagctgatt ctggagtgac ccagagggtc atgacttgag 1320cctaaaatcc gccgcctcca ccatttgtag aaaaatgtga cgaactcgtg agctctgtac 1380agtgaccggt gactctttct ggcatgcgga gagacggacg gacgcagaga gaagggctga 1440gtaataagcc actggccaga cagctctggc ggctctgagg tgcagtggat gattattaat 1500ccgggaccgg ccgcccctcc gccccgaagt ggaaaggctg gtgtgcccct cgttgaccaa 1560gaatctattg catcatcgga gaatatggag cttcatcgaa tcaccggcag taagcgaagg 1620agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag catgccatta acctaggtac 1680agaagtccaa ttgcttccga tctggtaaaa gattcacgag atagtacctt ctccgaagta 1740ggtagagcga gtacccggcg cgtaagctcc ctaattggcc catccggcat ctgtagggcg 1800tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa accggaaagg ccgctcagga 1860gctggccagc ggcgcagacc gggaacacaa gctggcagtc gacccatccg gtgctctgca 1920ctcgacctgc tgaggtccct cagtccctgg taggcagctt tgccccgtct gtccgcccgg 1980
tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac atttgttgcc atattttcct 2040gctctcccca ccagctgctc ttttcttttc tctttctttt cccatcttca gtatattcat 2100cttcccatcc aagaaccttt atttccccta agtaagtact ttgctacatc catactccat 2160ccttcccatc ccttattcct ttgaaccttt cagttcgagc tttcccactt catcgcagct 2220tgactaacag ctaccccgct tgagcagaca tcaccatgct gtcgaagctg cagtcaatca 2280gcgtcaaggc ccgccgcgtt gaactagccc gcgacatcac gcggcccaaa gtctgcctgc 2340atgctcagcg gtgctcgtta gttcggctgc gagtggcagc accacagaca gaggaggcgc 2400tgggaaccgt gcaggctgcc ggcgcgggcg atgagcacag cgccgatgta gcactccagc 2460agcttgaccg ggctatcgca gagcgtcgtg cccggcgcaa acgggagcag ctgtcatacc 2520aggctgccgc cattgcagca tcaattggcg tgtcaggcat tgccatcttc gccacctacc 2580tgagatttgc catgcacatg accgtgggcg gcgcagtgcc atggggtgaa gtggctggca 2640ctctcctctt ggtggttggt ggcgcgctcg gcatggagat gtatgcccgc tatgcacaca 2700aagccatctg gcatgagtcg cctctgggct ggctgctgca caagagccac cacacacctc 2760gcactggacc ctttgaagcc aacgacttgt ttgcaatcat caatggactg cccgccatgc 2820tcctgtgtac ctttggcttc tggctgccca acgtcctggg ggcggcctgc tttggagcgg 2880ggctgggcat cacgctatac ggcatggcat atatgtttgt acacgatggc ctggtgcaca 2940ggcgctttcc caccgggccc atcgctggcc tgccctacat gaagcgcctg acagtggccc 3000accagctaca ccacagcggc aagtacggtg gcgcgccctg gggtatgttc ttgggtccac 3060aggagctgca gcacattcca ggtgcggcgg aggaggtgga gcgactggtc ctggaactgg 3120actggtccaa gcggtagggt gcggaaccag gcacgctggt ttcacacctc atgcctgtga 3180taaggtgtgg ctagagcgat gcgtgtgaga cgggtatgtc acggtcgact ggtctgatgg 3240ccaatggcat cggccatgtc tggtcatcac gggctggttg cctgggtgaa ggtgatgcac 3300
atcatcatgt gcggttggag gggctggcac agtgtgggct gaactggagc agttgtccag 3360gctggcgttg aatcagtgag ggtttgtgat tggcggttgt gaagcaatga ctccgcccat 3420attctatttg tgggagctga gatgatggca tgcttgggat gtgcatggat catggtagtg 3480cagcaaacta tattcaccta gggctgttgg taggatcagg tgaggccttg cacattgcat 3540gatgtactcg tcatggtgtg ttggtgagag gatggatgtg gatggatgtg tattctcaga 3600cgtagacctt gactggaggc ttgatcgaga gagtgggccg tattctttga gaggggaggc 3660tcgtgccaga aatggtgagt ggatgactgt gacgctgtac attgcaggca ggtgagatgc 3720actgtctcga ttgtaaaata cattcagatg caagcttggc gtaatcatgg tcatagctgt 3780ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 3840agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 3900tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 3960cggggagagg cggtttgcgt attgggccaa agacaaaagg gcgacattca accgattgag 4020ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca ccgtcaccga 4080cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta ccattagcaa 4140ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta gcgacagaat 4200caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg gtcatagccc 4260ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag ccaccaccgg 4320aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca ccaccctcag 4380agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggc ccgatctagt 4440aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct 4500atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac 4560gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata 4620
atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa 4680cgatcgggga tcatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag 4740atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg 4800ccgggcccga tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc 4860gtaatgatat cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc 4920agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag 4980cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc 5040gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat 5100agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag 5160cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc 5220ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca 5280tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg tcgggcatgc 5340gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat 5400catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg 5460cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag 5520ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca 5580cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc 5640aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca 5700gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga 5760acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct 5820ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc 5880cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt ggataccgag 5940
gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg 6000accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa 6060actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg 6120taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc 6180tcatgatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata 6240ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 6300gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga 6360tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc 6420gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca gaatgccata 6480gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat 6540aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtat 6600gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt 6660ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg 6720acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc 6780gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt 6840tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg 6900gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg 6960aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc 7020ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc 7080gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact 7140gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg cggcggcacc 7200gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc 7260
ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg 7320aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc aggaccgctg 7380ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc 7440accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc 7500aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc gcttcctcgc 7560tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7620cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7680gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7740gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7800gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7860ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgcttttcc 7920gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc 7980gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg 8040cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact 8100gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct 8160accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag 8220ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag 8280gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa 8340atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg 8400ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc 8460ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc 8520aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa 8580
cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga 8640cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc gcctttgcga 8700cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg 8760cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc 8820tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg 8880actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga 8940gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga 9000tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg 9060acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc 9120acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc 9180gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa 9240accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg 9300cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg 9360cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt ccttgccatt 9420gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc cggaagcatt 9480gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag tgagggcggc 9540ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga cttcatggcg 9600gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc cgtgctcgtg 9660ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt 9720atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct 9780accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata 9840ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga tttcaggggg 9900
caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg 9960catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat 10020tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg 10080tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc 10140tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc 10200agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg 10260tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat 10320acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc 10380gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg 10440cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg 10500tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca 10560tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc 10620atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac 10680gcaccacccc gtcagtagct gaacaggagg gacagctgat agacacagaa gccactggag 10740cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cataattgtg 10800gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa 10860aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct 10920tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata 10980aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt 11040aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa 11100cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga 11160aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga 11220
acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga 11280agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag 11340gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt 11400agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga 11460agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc 11520cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga 11580tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga 11640cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct 11700attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact 11760ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag caggagcgca 11820ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg 11880gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg 11940gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg 12000caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc 12060cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg 12120acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc 12180cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc 12240gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt 12300cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag 12360gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg 12420aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt 12480ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc 12540
gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg 12600tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg 12660acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg 12720agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc 12780ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca 12840cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg 12900accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt 12960ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg 13020cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa 13080ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg 13140gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg 13200acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag 13260ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact tgcttcgctc 13320agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag gattaaaatt 13380gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc aggatttccg 13440cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg tttacgagca 13500cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg tggcattcgg 13560cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg acggccccaa 13620ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc gaggccgagg 13680ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg 13740acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc 13800gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac 13860
ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc 13920gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt 13980gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt 14040ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac 14100ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt 14160tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct 14220gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac 14280agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc cggggatgca 14340tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag caatggatag 14400gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc ttcctcagcg 14460gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca gcctgtcacg 14520gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg agatgatatt 14580tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc 14640atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga 14700gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc 14760ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg 14820gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga 14880cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg 14940cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc 15000aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat aaatcaaaag 15060aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 15120acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 15180
aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 15240ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 15300aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg ggaagggcga 15360tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 15420ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa 15480ttcgagctcg gtacccgggg atctttcgac actgaaatac gtcgagcctg ctccgcttgg 15540aagcggcgag gagcctcgtc ctgtcacaac taccaacatg gagtacgata agggccagtt 15600ccgccagctc attaagagcc agttcatggg cgttggcatg atggccgtca tgcatctgta 15660cttcaagtac accaacgctc ttctgatcca gtcgatcatc cgctgaaggc gctttcgaat 15720ctggttaaga tccacgtctt cgggaagcca gcgactggtg acctccagcg tccctttaag 15780gctgccaaca gctttctcag ccagggccag cccaagaccg acaaggcctc cctccagaac 15840gccgagaaga actggagggg tggtgtcaag gaggagtaag ctccttattg aagtcggagg 15900acggagcggt gtcaagagga tattcttcga ctctgtatta tagataagat gatgaggaat 15960tggaggtagc atagcttcat ttggatttgc tttccaggct gagactctag cttggagcat 16020agagggtcct ttggctttca atattctcaa gtatctcgag tttgaactta ttccctgtga 16080accttttatt caccaatgag cattggaatg aacatgaatc tgaggactgc aatcgccatg 16140aggttttcga aatacatccg gatgtcgaag gcttggggca cctgcgttgg ttgaatttag 16200aacgtggcac tattgatcat ccgatagctc tgcaaagggc gttgcacaat gcaagtcaaa 16260cgttgctagc agttccaggt ggaatgttat gatgagcatt gtattaaatc aggagatata 16320gcatgatctc tagttagctc accacaaaag tcagacggcg taaccaaaag tcacacaaca 16380caagctgtaa ggatttcggc acggctacgg aagacggaga agccaccttc agtggactcg 16440agtaccattt aattctattt gtgtttgatc gagacctaat acagccccta caacgaccat 16500
caaagtcgta tagctaccag tgaggaagtg gactcaaatc gacttcagca acatctcctg 16560gataaacttt aagcctaaac tatacagaat aagataggtg gagagcttat accgagctcc 16620caaatctgtc cagatcatgg ttgaccggtg cctggatctt cctatagaat catccttatt 16680cgttgaccta gctgattctg gagtgaccca gagggtcatg acttgagcct aaaatccgcc 16740gcctccacca tttgtagaaa aatgtgacga actcgtgagc tctgtacagt gaccggtgac 16800tctttctggc atgcggagag acggacggac gcagagagaa gggctgagta ataagccact 16860ggccagacag ctctggcggc tctgaggtgc agtggatgat tattaatccg ggaccggccg 16920cccctccgcc ccgaagtgga aaggctggtg tgcccctcgt tgaccaagaa tctattgcat 16980catcggagaa tatggagctt catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc 17040aggggtgtat agccgtcggc gaaatagcat gccattaacc taggtacaga agtccaattg 17100cttccgatct ggtaaaagat tcacgagata gtaccttctc cgaagtaggt agagcgagta 17160cccggcgcgt aagctcccta attggcccat ccggcatctg tagggcgtcc aaatatcgtg 17220cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc 17280gcagaccggg aacacaagct ggcagtcgac ccatccggtg ctctgcactc gacctgctga 17340ggtccctcag tccctggtag gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg 17400ttgacaaggt cgttgcgtca gtccaacatt tgttgccata ttttcctgct ctccccacca 17460gctgctcttt tcttttctct ttcttttccc atcttcagta tattcatctt cccatccaag 17520aacctttatt tcccctaagt aagtactttg ctacatccat actccatcct tcccatccct 17580tattcctttg aacctttcag ttcgagcttt cccacttcat cgcagcttga ctaacagcta 17640ccccgcttga gcagacatca ccatgcctga actcaccgcg acgtctgtcg agaagtttct 17700gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 17760tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 17820
tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 17880ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc 17940acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 18000cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 18060attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 18120tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 18180gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 18240gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 18300tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 18360gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 18420gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 18480tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 18540aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 18600cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 18660tcgtccgagg gcaaaggaat agagtagatg ccgaccgcgg gatcgatcca cttaacgtta 18720ctgaaatcat caaacagctt gacgaatctg gatataagat cgttggtgtc gatgtcagct 18780ccggagttga gacaaatggt gttcaggatc tcgataagat acgttcattt gtccaagcag 18840caaagagtgc cttctagtga tttaatagct ccatgtcaac aagaataaaa cgcgttttcg 18900ggtttacctc ttccagatac agctcatctg caatgcatta atgcattgac tgcaacctag 18960taacgccttn caggctccgg cgaagagaag aatagcttag cagagctatt ttcattttcg 19020ggagacgaga tcaagcagat caacggtcgt caagagacct acgagactga ggaatccgct 19080cttggctcca cgcgactata tatttgtctc taattgtact ttgacatgct cctcttcttt 19140
actctgatag cttgactatg aaaattccgt caccagcncc tgggttcgca aagataattg 19200catgtttctt ccttgaactc tcaagcctac aggacacaca ttcatcgtag gtataaacct 19260cgaaatcant tcctactaag atggtataca atagtaacca tgcatggttg cctagtgaat 19320gctccgtaac acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta 19380cccagaatgc acaggtacac ttgtttagag gtaatccttc tttctagcta gaagtcctcg 19440tgtactgtgt aagcgcccac tccacatctc cactcgacct gcaggcatgc a 19491<210>46<211>21300<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(3471)..(3471)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3679)..(3679)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3770)..(3770)<223>n為a，c，g，或t<400>46gatctttcga cactgaaata cgtcgagcct gctccgcttg gaagcggcga ggagcctcgt 60cctgtcacaa ctaccaacat ggagtacgat aagggccagt tccgccagct cattaagagc120cagttcatgg gcgttggcat gatggccgtc atgcatctgt acttcaagta caccaacgct180
cttctgatcc agtcgatcat ccgctgaagg cgctttcgaa tctggttaag atccacgtct240tcgggaagcc agcgactggt gacctccagc gtccctttaa ggctgccaac agctttctca300gccagggcca gcccaagacc gacaaggcct ccctccagaa cgccgagaag aactggaggg360gtggtgtcaa ggaggagtaa gctccttatt gaagtcggag gacggagcgg tgtcaagagg420atattcttcg actctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca480tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc tttggctttc540aatattctca agtatctcga gtttgaactt attccctgtg aaccttttat tcaccaatga600gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc660ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca720tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg780tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct840caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg900cacggctacg gaagacggag aagccacctt cagtggactc gagtaccatt taattctatt960tgtgtttgat cgagacctaa tacagcccct acaacgacca tcaaagtcgt atagctacca 1020gtgaggaagt ggactcaaat cgacttcagc aacatctcct ggataaactt taagcctaaa 1080ctatacagaa taagataggt ggagagctta taccgagctc ccaaatctgt ccagatcatg 1140gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct 1200ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa 1260aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga 1320gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca gctctggcgg 1380ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc cccgaagtgg 1440aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga atatggagct 1500
tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta tagccgtcgg 1560cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc tggtaaaaga 1620ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg taagctccct 1680aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg 1740tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc 1800tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta 1860ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc 1920agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc 1980tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag 2040taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca 2100gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc 2160accatgcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 2220gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 2280ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 2340tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 2400gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 2460gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg 2520atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 2580ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 2640tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 2700atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 2760aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 2820
ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 2880atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg 2940ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 3000aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 3060gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 3120gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa 3180tagagtagat gccgaccgcg ggatcgatcc acttaacgtt actgaaatca tcaaacagct 3240tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg 3300tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg 3360atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata 3420cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg 3480gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga 3540tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat 3600atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat 3660gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact 3720ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa 3780gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac 3840gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca 3900cttgtttaga ggtaatcctt ctttctagct agaagtcctc gtgtactgtg taagcgccca 3960ctccacatct ccactcgacc tgcaggcatg caagcttgaa ttcgagctcg gtacccgggg 4020atctttcgac actgaaatac gtcgagcctg ctccgcttgg aagcggcgag gagcctcgtc 4080ctgtcacaac taccaacatg gagtacgata agggccagtt ccgccagctc attaagagcc 4140
agttcatggg cgttggcatg atggccgtca tgcatctgta cttcaagtac accaacgctc 4200ttctgatcca gtcgatcatc cgctgaaggc gctttcgaat ctggttaaga tccacgtctt 4260cgggaagcca gcgactggtg acctccagcg tccctttaag gctgccaaca gctttctcag 4320ccagggccag cccaagaccg acaaggcctc cctccagaac gccgagaaga actggagggg 4380tggtgtcaag gaggagtaag ctccttattg aagtcggagg acggagcggt gtcaagagga 4440tattcttcga ctctgtatta tagataagat gatgaggaat tggaggtagc atagcttcat 4500ttggatttgc tttccaggct gagactctag cttggagcat agagggtcct ttggctttca 4560atattctcaa gtatctcgag tttgaactta ttccctgtga accttttatt caccaatgag 4620cattggaatg aacatgaatc tgaggactgc aatcgccatg aggttttcga aatacatccg 4680gatgtcgaag gcttggggca cctgcgttgg ttgaatttag aacgtggcac tattgatcat 4740ccgatagctc tgcaaagggc gttgcacaat gcaagtcaaa cgttgctagc agttccaggt 4800ggaatgttat gatgagcatt gtattaaatc aggagatata gcatgatctc tagttagctc 4860accacaaaag tcagacggcg taaccaaaag tcacacaaca caagctgtaa ggatttcggc 4920acggctacgg aagacggaga agccaccttc agtggactcg agtaccattt aattctattt 4980gtgtttgatc gagacctaat acagccccta caacgaccat caaagtcgta tagctaccag 5040tgaggaagtg gactcaaatc gacttcagca acatctcctg gataaacttt aagcctaaac 5100tatacagaat aagataggtg gagagcttat accgagctcc caaatctgtc cagatcatgg 5160ttgaccggtg cctggatctt cctatagaat catccttatt cgttgaccta gctgattctg 5220gagtgaccca gagggtcatg acttgagcct aaaatccgcc gcctccacca tttgtagaaa 5280aatgtgacga actcgtgagc tctgtacagt gaccggtgac tctttctggc atgcggagag 5340acggacggac gcagagagaa gggctgagta ataagccact ggccagacag ctctggcggc 5400tctgaggtgc agtggatgat tattaatccg ggaccggccg cccctccgcc ccgaagtgga 5460
aaggctggtg tgcccctcgt tgaccaagaa tctattgcat catcggagaa tatggagctt 5520catcgaatca ccggcagtaa gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc 5580gaaatagcat gccattaacc taggtacaga agtccaattg cttccgatct ggtaaaagat 5640tcacgagata gtaccttctc cgaagtaggt agagcgagta cccggcgcgt aagctcccta 5700attggcccat ccggcatctg tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt 5760gtatgaaacc ggaaaggccg ctcaggagct ggccagcggc gcagaccggg aacacaagct 5820ggcagtcgac ccatccggtg ctctgcactc gacctgctga ggtccctcag tccctggtag 5880gcagctttgc cccgtctgtc cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca 5940gtccaacatt tgttgccata ttttcctgct ctccccacca gctgctcttt tcttttctct 6000ttcttttccc atcttcagta tattcatctt cccatccaag aacctttatt tcccctaagt 6060aagtactttg ctacatccat actccatcct tcccatccct tattcctttg aacctttcag 6120ttcgagcttt cccacttcat cgcagcttga ctaacagcta ccccgcttga gcagacatca 6180ccatgtcaat actcacttat ctggaatttc atctctacta tacactacct gtccttgcgg 6240cattgtgttg gctgctaaag ccgtttcact cacagcaaga caatctcaag tataaatttt 6300taatgttgat ggccgcctct accgcatcga tttgggacaa ttatatcgtt tatcatcgcg 6360cttggtggta ctgtcctact tgtgttgtgg ctgtcattgg ctatgtacct ctagaagaat 6420acatgttctt tatcatcatg actttaatga ctgtcgcgtt ctcaaacttt gttatgcgtt 6480ggcacttgca tactttcttt attagaccca acacttcttg gaagcaaaca ctattagtac 6540gccttgtgcc tgtttcagct ttattggcaa tcacttatca tgcttggcac ttgacactgc 6600caaataaacc ttcattttat ggttcatgca tcctttggta tgcttgtcct gtgttggcta 6660ttctttggct gggtgctggc gaatatatct tgcgtcgacc tgtggctgtc cttttgtcta 6720ttgttatccc tagtgtatac ctatgttggg ctgatatcgt cgctattagt gctggcacat 6780
ggcatatttc tcttagaaca agcactggca aaatggtagt acccgattta cctgtagaag 6840aatgcctgtt ttttactttg atcaacacag tcttggtttt tgctacctgt gctatagacc 6900gcgctcaggc catcctccat gtgagcgcgc gtaatacgac tcactatagg gcgaattgga 6960gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcgg 7020cacgagctac atttcacaag cccgtgagcg gtgcaagcgc tctgccccac atcggcccac 7080ctcctcatct ccatcggtca tttgctgcta ccacgatgct gtcgaagctg cagtcaatca 7140gcgtcaaggc ccgccgcgtt gaactagccc gcgacatcac gcggcccaaa gtctgcctgc 7200atgctcagcg gtgctcgtta gttcggctgc gagtggcagc accacagaca gaggaggcgc 7260tgggaaccgt gcaggctgcc ggcgcgggcg atgagcacag cgccgatgta gcactccagc 7320agcttgaccg ggctatcgca gagcgtcgtg cccggcgcaa acgggagcag ctgtcatacc 7380aggctgccgc cattgcagca tcaattggcg tgtcaggcat tgccatcttc gccacctacc 7440tgagatttgc catgcacatg accgtgggcg gcgcagtgcc atggggtgaa gtggctggca 7500ctctcctctt ggtggttggt ggcgcgctcg gcatggagat gtatgcccgc tatgcacaca 7560aagccatctg gcatgagtcg cctctgggct ggctgctgca caagagccac cacacacctc 7620gcactggacc ctttgaagcc aacgacttgt ttgcaatcat caatggactg cccgccatgc 7680tcctgtgtac ctttggcttc tggctgccca acgtcctggg ggcggcctgc tttggagcgg 7740ggctgggcat cacgctatac ggcatggcat atatgtttgt acacgatggc ctggtgcaca 7800ggcgctttcc caccgggccc atcgctggcc tgccctacat gaagcgcctg acagtggccc 7860accagctaca ccacagcggc aagtacggtg gcgcgccctg gggtatgttc ttgggtccac 7920aggagctgca gcacattcca ggtgcggcgg aggaggtgga gcgactggtc ctggaactgg 7980actggtccaa gcgggctcag gccatcctcc atctgtacaa atcatctgtt caaaatcaaa 8040accctaaaca agccatttcc cttttccagc atgtcaaaga gctagcatgg gccttctgtc 8100
ttcctgacca aatgctcaac aatgaattgt ttgatgatct tactatcagc tgggatattt 8160tacgtaaagc ctcaaagtca ttctatactg catctgccgt ttttccaagt tatgtacgtc 8220aagacttggg tgttctctat gctttctgca gagctaccga tgacctgtgc gatgatgaat 8280ccaaatctgt tcaagaaaga agagaccaat tagatcttac tcgacaattt gttcgtgatc 8340tctttagcca aaagaccagt gcgcctattg tgattgattg ggaattgtat caaaaccaac 8400ttcctgcttc ttgtatatca gcctttagag cctttactcg ccttcgccat gtccttgaag 8460tagaccctgt agaagaacta ttagatggtt acaaatggga tcttgagcgt cgtcctatcc 8520ttgatgaaca agacttggag gcatactctg cttgtgtggc cagtagtgtg ggtgaaatgt 8580gcacacgtgt gattcttgct caagaccaaa aggaaaatga tgcttggata attgaccgtg 8640cacgtgagat ggggctggtg ctacaatacg ttaacattgc tcgagacatt gtgactgata 8700gcgagactct gggtcgatgt tatctgcctc aacaatggct tagaaaagaa gaaacagaac 8760aaatacagca aggcaacgcc cgtagcctag gtgatcaaag actgttgggc ttgtctctga 8820agcttgtagg aaaggcagac gctatcatgg tgagagctaa gaagggcatt gacaagttgc 8880cggcaaactg tcaaggcggt gtacgagctg cttgccaagt atatgctgca attggatctg 8940tactcaagca gcagaagaca acatatccta caagagctca tctaaaagga agcgaacgtg 9000ccaagattgc tctgttgagt gtatacaacc tctatcaatc tgaagacaag cctgtggctc 9060tccgtcaagc tagaaagatt aagagttttt ttgttgatta gtgaattttt gttttattta 9120tgtctgatag ttcaataaag agacaacaca tacaatataa aatcattgtc tttaaatgtt 9180aatttagtag agtgtaaagc ctgcattttt tttgtacgca taaacaatga gttcaccccg 9240cttctggttt ttaaataatt atgtcaaact agggaaaatt cttttttttc tcttcgttct 9300ttttttggct tgttgtggag tcacaggctt gtcttcagat tgatagaggt tgtatacact 9360caacagagca atcttggcac gttcgcttcc ttttagatga gctcttgtag gatatgttgt 9420
cttctgctgc ttgagtacag atccaattgc agcatatact tggcaagcag ctcgtacacc 9480gccttgacag tttgccggca acttgtcaat gcccttctta gctctcacca tgatagcgtc 9540tgcctttcct acaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 9600tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 9660ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 9720aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 9780tattgggcca aagacaaaag ggcgacattc aaccgattga gggagggaag gtaaatattg 9840acggaaatta ttcattaaag gtgaattatc accgtcaccg acttgagcca tttgggaatt 9900agagccagca aaatcaccag tagcaccatt accattagca aggccggaaa cgtcaccaat 9960gaaaccatcg atagcagcac cgtaatcagt agcgacagaa tcaagtttgc ctttagcgtc 10020agactgtagc gcgttttcat cggcattttc ggtcatagcc cccttattag cgtttgccat 10080cttttcataa tcaaaatcac cggaaccaga gccaccaccg gaaccgcctc cctcagagcc 10140gccaccctca gaaccgccac cctcagagcc accaccctca gagccgccac cagaaccacc 10200accagagccg ccgccagcat tgacaggagg cccgatctag taacatagat gacaccgcgc 10260gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata 10320attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa 10380ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa 10440caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg atcatccggg 10500tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg tgcatctcgg 10560tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgggcccg atcatattgt 10620cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt cgtaatgata tcggcacctt 10680cgaccgcctg ttccgcagag atcccgtggg cgaagaactc cagcatgaga tccccgcgct 10740
ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg 10800cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga 10860accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga 10920atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 10980ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 11040gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 11100atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga gcctggcgaa 11160cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 11220ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca 11280ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 11340ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 11400gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 11460cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt 11520cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 11580gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 11640acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccagatccgg tgcagattat 11700ttggattgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca 11760gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa 11820cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct 11880gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg 11940tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatca gattgtcgtt 12000tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga 12060
gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt 12120cgtccatttg tatgtgcatg ccaaccacag ggttccccag atctggcgcc ggccagcgag 12180acgagcaaga ttggccgccg cccgaaacga tccgacagcg cgcccagcac aggtgcgcag 12240gcaaattgca ccaacgcata cagcgccagc agaatgccat agtgggcggt gacgtcgttc 12300gagtgaacca gatcgcgcag gaggcccggc agcaccggca taatcaggcc gatgccgaca 12360gcgtcgagcg cgacagtgct cagaattacg atcaggggta tgttgggttt cacgtctggc 12420ctccggacca gcctccgctg gtccgattga acgcgcggat tctttatcac tgataagttg 12480gtggacatat tatgtttatc agtgataaag tgtcaagcat gacaaagttg cagccgaata 12540cagtgatccg tgccgccctg gacctgttga acgaggtcgg cgtagacggt ctgacgacac 12600gcaaactggc ggaacggttg ggggttcagc agccggcgct ttactggcac ttcaggaaca 12660agcgggcgct gctcgacgca ctggccgaag ccatgctggc ggagaatcat acgcattcgg 12720tgccgagagc cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc agcttcaggc 12780aggcgctgct cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga ccgggcgcac 12840cgcagatgga aacggccgac gcgcagcttc gcttcctctg cgaggcgggt ttttcggccg 12900gggacgccgt caatgcgctg atgacaatca gctacttcac tgttggggcc gtgcttgagg 12960agcaggccgg cgacagcgat gccggcgagc gcggcggcac cgttgaacag gctccgctct 13020cgccgctgtt gcgggccgcg atagacgcct tcgacgaagc cggtccggac gcagcgttcg 13080agcagggact cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt gtcaggaacg 13140ttgaaggacc gagaaagggt gacgattgat caggaccgct gccggagcgc aacccactca 13200ctacagcaga gccatgtaga caacatcccc tccccctttc caccgcgtca gacgcccgta 13260gcagcccgct acgggctttt tcatgccctg ccctagcgtc caagcctcac ggccgcgctc 13320ggcctctctg gcggccttct ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 13380
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 13440agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 13500ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 13560caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 13620gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 13680cctgtccgcc tttctccctt cgggaagcgt ggcgcttttc cgctgcataa ccctgcttcg 13740gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt 13800gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag 13860gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg 13920cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg 13980agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcaagg 14040tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga 14100gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc gtcgtggact 14160atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg ggcggcctgc 14220tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc acgatcctcg 14280ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg atgggcgtgg 14340tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg ggtgcgcgtg 14400attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtgaag 14460tacatcaccg acgagcaagg caagaccgag cgcctttgcg acgctcaccg ggctggttgc 14520cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa 14580gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct 14640cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt 14700
gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat 14760cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat 14820aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc 14880cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg 14940gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg 15000ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg 15060ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct 15120cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg 15180gcctcacccc aaaaatggca gcgctggcag tccttgccat tgccgggatc ggggcagtaa 15240cgggatgggc gatcagcccg agcgcgacgc ccggaagcat tgacgtgccg caggtgctgg 15300catcgacatt cagcgaccag gtgccgggca gtgagggcgg cggcctgggt ggcggcctgc 15360ccttcacttc ggccgtcggg gcattcacgg acttcatggc ggggccggca atttttacct 15420tgggcattct tggcatagtg gtcgcgggtg ccgtgctcgt gttcgggggt gcgataaacc 15480cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac 15540ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga 15600agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct 15660tttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc 15720ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa 15780cccaggacaa taaccttata gcttgtaaat tctatcataa ttgggtaatg actccaactt 15840attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgcagc tccaccgatt 15900ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat 15960gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc ttcaggcggg 16020
attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt gacagcaggc 16080tcataagacg ccccagcgtc gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct 16140tccggagact gtcatacgcg taaaacagcc agcgctggcg cgatttagcc ccgacatagc 16200cccactgttc gtccatttcc gcgcagacga tgacgtcact gcccggctgt atgcgcgagg 16260ttaccgactg cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc caacgcccat 16320aatgcgggct gttgcccggc atccaacgcc attcatggcc atatcaatga ttttctggtg 16380cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga 16440gcagagatag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc 16500tgaacaggag ggacagctga tagacacaga agccactgga gcacctcaaa aacaccatca 16560tacactaaat cagtaagttg gcagcatcac ccataattgt ggtttcaaaa tcggctccgt 16620cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt 16680aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg 16740gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata 16800tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg 16860tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg 16920gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg 16980ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc 17040aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa 17100agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac 17160atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta 17220ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa 17280gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt 17340
tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt 17400attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg 17460tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg 17520atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac 17580ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa 17640gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt 17700cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac 17760cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca 17820ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa 17880tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga 17940ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc 18000cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc 18060tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga 18120ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa 18180gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt 18240gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc 18300gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac 18360gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa 18420ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg 18480gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt 18540ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga 18600ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca 18660
cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc 18720ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac 18780gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta 18840tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg 18900atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt 18960gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg 19020ctagggcctt gtggggtcag ttccggctgg gggttcagca gccagcgctt tactggcatt 19080tcaggaacaa gcgggcactg ctcgacgcac ttgcttcgct cagtatcgct cgggacgcac 19140ggcgcgctct acgaactgcc gataaacaga ggattaaaat tgacaattgt gattaaggct 19200cagattcgac ggcttggagc ggccgacgtg caggatttcc gcgagatccg attgtcggcc 19260ctgaagaaag ctccagagat gttcgggtcc gtttacgagc acgaggagaa aaagcccatg 19320gaggcgttcg ctgaacggtt gcgagatgcc gtggcattcg gcgcctacat cgacggcgag 19380atcattgggc tgtcggtctt caaacaggag gacggcccca aggacgctca caaggcgcat 19440ctgtccggcg ttttcgtgga gcccgaacag cgaggccgag gggtcgccgg tatgctgctg 19500cgggcgttgc cggcgggttt attgctcgtg atgatcgtcc gacagattcc aacgggaatc 19560tggtggatgc gcatcttcat cctcggcgca cttaatattt cgctattctg gagcttgttg 19620tttatttcgg tctaccgcct gccgggcggg gtcgcggcga cggtaggcgc tgtgcagccg 19680ctgatggtcg tgttcatctc tgccgctctg ctaggtagcc cgatacgatt gatggcggtc 19740ctgggggcta tttgcggaac tgcgggcgtg gcgctgttgg tgttgacacc aaacgcagcg 19800ctagatcctg tcggcgtcgc agcgggcctg gcgggggcgg tttccatggc gttcggaacc 19860gtgctgaccc gcaagtggca acctcccgtg cctctgctca cctttaccgc ctggcaactg 19920gcggccggag gacttctgct cgttccagta gctttagtgt ttgatccgcc aatcccgatg 19980
cctacaggaa ccaatgttct cggcctggcg tggctcggcc tgatcggagc gggtttaacc 20040tacttccttt ggttccgggg gatctcgcga ctcgaaccta cagttgtttc cttactgggc 20100tttctcagcc ccagatctgg ggtcgatcag ccggggatgc atcaggccga cagtcggaac 20160ttcgggtccc cgacctgtac cattcggtga gcaatggata ggggagttga tatcgtcaac 20220gttcacttct aaagaaatag cgccactcag cttcctcagc ggctttatcc agcgatttcc 20280tattatgtcg gcatagttct caagatcgac agcctgtcac ggttaagcga gaaatgaata 20340agaaggctga taattcggat ctctgcgagg gagatgatat ttgatcacag gcagcaacgc 20400tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg 20460cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac 20520aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt 20580tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt 20640aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg 20700tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct 20760gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga 20820tggtggttcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt 20880gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 20940agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag 21000ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 21060tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 21120agcgggcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 21180tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 21240ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg 21300
<210>47<211>17756<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>47ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540
tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa 1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag 1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata 1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc 1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga 1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta 1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc 1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc 1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt 1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag 1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc 1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta 1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc 1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga 1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa 1860
tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt 1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca 1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc 2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca 2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg 2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat 2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc 2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga 2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata 2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga 2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca 2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta 2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt 2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga 2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga 2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga 2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa 2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc 2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt 3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga 3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact 3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg 3180
ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga 3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag 3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag 3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg 3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg 3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca 3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc 3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta 3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca 3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt 3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg 3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt 3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg 3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga 4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt 4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg 4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg 4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg 4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac 4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc 4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag 4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg 4500
tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg 4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc 4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat 4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat 4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga 4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta 4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc 4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc 4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat 5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt 5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg 5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg 5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac 5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat 5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac 5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc 5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg 5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt 5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc 5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt 5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta 5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag 5820
cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca 5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc 6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt 6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag 6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg 6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca 6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa 6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt 7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc 7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag 7140
aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag 7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg 7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg 7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt 7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt 7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg 7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc 7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga 7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct 7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc 7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt 7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa 7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc 7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga 7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc 8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc 8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag 8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc 8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg 8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt 8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg 8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc 8460
gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc 8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac 8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc 8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca 8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct 8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt 8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc 8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc 8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780
cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa 10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag 10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga 10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta 10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc 10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac 10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc 10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg 10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt 10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat 10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg 10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga 10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact 10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt cattttgctt 10800tgtaaatttc tggtaactgc caccaagaaa tatgaggata ttcgtgatgt tcctcgtggt 10860agccaaaatg atagcacgtg ataaatgacc accaaatagg acggctaatt gtttgggcac 10920aatgaggctg aacataaccc cctattggtt cactatgggg taaaaaagta ccaaaataga 10980ataattgtaa tgaacttaaa agcgagggta gcacccaaaa gtaagttaga ttatcacttg 11040ggatatggag tatgtattta gcaaagttat aaataatagt caacgcaatt atttgccccc 11100
aactccagta acctttcata aaatgaaaat accaagcaaa gaaactttgg tgtttaccat 11160tgtgaaaatc cgggtctatt gagcttgctg gattgtggtg gtgtaaccaa tgttttttca 11220atagtttttg atatggtaaa agaccataaa gggatagggt caatgttcca atcaaatgat 11280taatcttggt gttttgggga aatactacgc catgcatggc atcatgagat gtaataaata 11340atcccgtata taaaaatgtt tgccatagta taacaggcaa taacatccaa aattttagct 11400ttgagatgtc aagggaaagt aataaactca ggctaatgac ccatgcgcta acaatgacaa 11460tagcaatgaa aagcccctta aactgagatt tacttctcag tactggagtc agttttgctt 11520gatgactgag tggttgttct aactggatca tttctaaaga gaaggtggaa caatgttagc 11580ataattgtgc ttgagtgagg actttgaggg taggtacata cttgataaag ttaatgatta 11640aagagaaaaa aaaagttttg gttcaaagca gaaattgttt tttaaatcga ttggtgagaa 11700aatttttttc tgtttccgca tcaccaaagc cacctcagga atggtcacaa attattggtc 11760tgattggacc ataagcatac aaaaagttca ttgaagtata cttagtggct tattagactt 11820ttatcgtttt ctaacgcgaa tcagcaatgt ttcttgtttg atttactgct tgctttagat 11880catttttgtc tgaaatatta tgcatttgtt caaagcggcc tttgtttcct ttctttcatg 11940cttaaacacg ttgtttattc catatattac tttgaatatg catcaccgca aagcggaagt 12000gcaaaataac aaagaacctc tttgggttac acgatcaact gctattgtga aaaaaatttc 12060tttttgaaaa tttttggaat aatatctctt gcaaaaaaga aattttgtat atttagtagc 12120atcaagaaca aatgaaagaa gtgtgggata acaagaatac atcatcttta gacaaaagta 12180cgagaaaaat ctaataagtt gttatagagg tctttgtttt ctttgtgttt atagacagtt 12240atttagagtt tgaaaagtgt ctctaatgtg tcttttttta ttattattat ttcaaatgtt 12300atgtaatata gctaaagcta tagatttgac attttttcta aatataaaat ttcagtcaac 12360agaaataaat gacacgagtt ctttttctct ctctcaatcc tgttgatcat caatctttga 12420
tgtcgtttta aaacaaatga atggcattta gttccttagg tgtcactcac atcttgttga 12480ccagaaaatc cttattcgcc ctcaaatctg ctttattcct ttcatttgat ttgatgttta 12540agtaatgcaa gcaaacaaaa aagaaacctt tcttgcaaag acaaaagaat tgttttcaga 12600ggaaagcaac tcgttgtcat tttttaagga tttagactta taatcgacac catagtttgt 12660ccgttacatt ttttattgtc gttttctgat ttccttttaa tctttaagca aaatcaatat 12720taacttatct tgtcttccaa taaaaaatgg ataccaataa caataaatcc ttcacaaaga 12780aaaaaaaaaa aaactcgaaa aaagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 12840aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 12900ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 12960ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 13020cggtttgcgt attgggccaa agacaaaagg gcgacattca accgattgag ggagggaagg 13080taaatattga cggaaattat tcattaaagg tgaattatca ccgtcaccga cttgagccat 13140ttgggaatta gagccagcaa aatcaccagt agcaccatta ccattagcaa ggccggaaac 13200gtcaccaatg aaaccatcga tagcagcacc gtaatcagta gcgacagaat caagtttgcc 13260tttagcgtca gactgtagcg cgttttcatc ggcattttcg gtcatagccc ccttattagc 13320gtttgccatc ttttcataat caaaatcacc ggaaccagag ccaccaccgg aaccgcctcc 13380ctcagagccg ccaccctcag aaccgccacc ctcagagcca ccaccctcag agccgccacc 13440agaaccacca ccagagccgc cgccagcatt gacaggaggc ccgatctagt aacatagatg 13500acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt 13560aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt 13620acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa 13680gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga 13740
tcatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt13800gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga13860tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat13920cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat13980ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt14040catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg14100tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat14160gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc14220gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac14280acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg14340caagcaggca tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag14400cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc14460gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc14520gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga14580tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa14640tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc14700cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga14760caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc14820atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc14880ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt14940gcagattatt tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat15000ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg15060
acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa15120cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct15180tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcag15240attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta15300aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt15360ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga tctggcgccg15420gccagcgaga cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca15480ggtgcgcagg caaattgcac caacgcatac agcgccagca gaatgccata gtgggcggtg15540acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg15600atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtat gttgggtttc15660acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact15720gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc15780agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc15840tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact15900tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata15960cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca16020gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac16080cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt16140tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg16200tgcttgagga gcaggccggc gacagcgatg ccggcgagcg cggcggcacc gttgaacagg16260ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg16320cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg16380
tcaggaacgt tgaaggaccg agaaagggtg acgattgatc aggaccgctg ccggagcgca16440acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc accgcgtcag16500acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc aagcctcacg16560gccgcgctcg gcctctctgg cggccttctg gcgctcttcc gcttcctcgc tcactgactc16620gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg16680gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa16740ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga16800cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag16860ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct16920taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgcttttcc gctgcataac16980cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata17040caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg17100gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt17160cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg17220taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac17280ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg17340ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg17400tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg17460gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca17520cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc aaggtcatga17580tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa cggccggggg17640gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga cttcgcggag17700
ctggtgaagt acatcaccga cgagcaaggc aagaccgagc gcctttgcga cgctca 17756<210>48<211>17118<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>48ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg 120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac 180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc 240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga 300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat 360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat 420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt 480
ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg 540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc 600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc 660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg 720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg 780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg 840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg 900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg 960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga1800
ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact3120
tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag4440
cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta5760
tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc7080
aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg8400
atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc9720
aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt gagattaaaa10800tagataagga aaagaaagtg aaaagaaatt cggaagcatg gcacattctt ctttttataa10860atacatgcct gactttcttt ttccatcgat atgatatatg catatgatag atatacaagc10920aatcttcttc aaggagtttg aaattttgtc ctccaggagc aaaaaaaagt ttttttttat10980acatgtttgt acacaagaat agttaccaat ttgctttggt cttacgtgct gcaagtttat11040
atcgttttca atttctttgt ctttacattt tctttgtcct ttatctttcc tcatttagtc11100tttgggagaa ttaggaaaag ggagcggaaa ggtaagaaat gcttgcgtat tttactaatt11160cggcaaacat ccaatttggc aaacagcagc ctgtgcaacg ctctcgagat gacagtatct11220ttgattacac tctaaatctc gatgacccga ccaaaaagag cgaacaaaga aataatcttg11280tgcattcgaa tatgatggaa gattttttcc cccttattct aaatgttgac atagcgtgta11340tgttatataa acaaaaagaa attgtacaaa ctttcttttc ttctcttttt attttatctc11400tatgatccag ttagaacaac cactcagtca tcaagcaaaa ctgactccag tactgagaag11460taaatctcag tttaaggggc ttttcattgc tattgtcatt gttagcgcat gggtcattag11520cctgagttta ttactttccc ttgacatctc aaagctaaaa ttttggatgt tattgcctgt11580tatactatgg caaacatttt tatatacggg attatttatt acatctcatg atgccatgca11640tggcgtagta tttccccaaa acaccaagat taatcatttg attggaacat tgaccctatc11700cctttatggt cttttaccat atcaaaaact attgaaaaaa cattggttac accaccacaa11760tccagcaagc tcaatagacc cggattttca caatggtaaa caccaaagtt tctttgcttg11820gtattttcat tttatgaaag gttactggag ttgggggcaa ataattgcgt tgactattat11880ttataacttt gctaaataca tactccatat cccaagtgat aatctaactt acttttgggt11940gctaccctcg cttttaagtt cattacaatt attctatttt ggtacttttt taccccatag12000tgaaccaata gggggttatg ttcagcctca ttgtgcccaa acaattagcc gtcctatttg12060gtggtcattt atcacgtgct atcattttgg ctaccacgag gaacatcacg aatatcctca12120tatttcttgg tggcagttac cagaaattta caaagcaaaa tagaagcttg gcgtaatcat12180ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag12240ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg12300cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa12360
tcggccaacg cgcggggaga ggcggtttgc gtattgggcc aaagacaaaa gggcgacatt12420caaccgattg agggagggaa ggtaaatatt gacggaaatt attcattaaa ggtgaattat12480caccgtcacc gacttgagcc atttgggaat tagagccagc aaaatcacca gtagcaccat12540taccattagc aaggccggaa acgtcaccaa tgaaaccatc gatagcagca ccgtaatcag12600tagcgacaga atcaagtttg cctttagcgt cagactgtag cgcgttttca tcggcatttt12660cggtcatagc ccccttatta gcgtttgcca tcttttcata atcaaaatca ccggaaccag12720agccaccacc ggaaccgcct ccctcagagc cgccaccctc agaaccgcca ccctcagagc12780caccaccctc agagccgcca ccagaaccac caccagagcc gccgccagca ttgacaggag12840gcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat12900attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat12960ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga13020aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc13080caaatgtttg aacgatcggg gatcatccgg gtctgtggcg ggaactccac gaaaatatcc13140gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg13200ttgatgtgga cgccgggccc gatcatattg tcgctcagga tcgtggcgtt gtgcttgtcg13260gccgttgctg tcgtaatgat atcggcacct tcgaccgcct gttccgcaga gatcccgtgg13320gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa13380acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc13440aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt13500caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga13560ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta13620tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc13680
cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcatcgc13740cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct13800cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga13860tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc13920gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat13980cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga14040gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct14100gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg14160ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc14220cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca14280tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat gagactctaa14340ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc14400tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc14460ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt14520cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc14580ttaattctcc gctcatgatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt14640tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat14700atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca14760gggttcccca gatctggcgc cggccagcga gacgagcaag attggccgcc gcccgaaacg14820atccgacagc gcgcccagca caggtgcgca ggcaaattgc accaacgcat acagcgccag14880cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca ggaggcccgg14940cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc tcagaattac15000
gatcaggggt atgttgggtt tcacgtctgg cctccggacc agcctccgct ggtccgattg15060aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat cagtgataaa15120gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct ggacctgttg15180aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt gggggttcag15240cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc actggccgaa15300gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga ctggcgctca15360tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg cgatggcgcg15420cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga cgcgcagctt15480cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct gatgacaatc15540agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga tgccggcgag15600cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc gatagacgcc15660ttcgacgaag ccggtccgga cgcagcgttc gagcagggac tcgcggtgat tgtcgatgga15720ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg tgacgattga15780tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag acaacatccc15840ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt ttcatgccct15900gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc tggcgctctt15960ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag16020ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca16080tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt16140tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc16200gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct16260ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg16320
tggcgctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat16380ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg16440tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt16500tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga16560ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag16620ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga acgaagagcg16680attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct ggccgtcggc16740cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct ggcccgcatc16800aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga cgacccgcgc16860acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga agagaagcag16920gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc atgacttttt16980tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca tgcgctccat17040caagaagagc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag gcaagaccga17100gcgcctttgc gacgctca 17118<210>49<211>18449<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(3471)..(3471)<223>n為a，c，g，或t
<220>
<221>misc_feature<222>(3679)..(3679)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3770)..(3770)<223>n為a，c，g，或t<400>49gatctttcga cactgaaata cgtcgagcct gctccgcttg gaagcggcga ggagcctcgt 60cctgtcacaa ctaccaacat ggagtacgat aagggccagt tccgccagct cattaagagc 120cagttcatgg gcgttggcat gatggccgtc atgcatctgt acttcaagta caccaacgct 180cttctgatcc agtcgatcat ccgctgaagg cgctttcgaa tctggttaag atccacgtct 240tcgggaagcc agcgactggt gacctccagc gtccctttaa ggctgccaac agctttctca 300gccagggcca gcccaagacc gacaaggcct ccctccagaa cgccgagaag aactggaggg 360gtggtgtcaa ggaggagtaa gctccttatt gaagtcggag gacggagcgg tgtcaagagg 420atattcttcg actctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca 480tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc tttggctttc 540aatattctca agtatctcga gtttgaactt attccctgtg aaccttttat tcaccaatga 600gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc 660ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca 720tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg 780tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct 840caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg 900cacggctacg gaagacggag aagccacctt cagtggactc gagtaccatt taattctatt 960tgtgtttgat cgagacctaa tacagcccct acaacgacca tcaaagtcgt atagctacca1020
gtgaggaagt ggactcaaat cgacttcagc aacatctcct ggataaactt taagcctaaa1080ctatacagaa taagataggt ggagagctta taccgagctc ccaaatctgt ccagatcatg1140gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct1200ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa1260aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga1320gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca gctctggcgg1380ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc cccgaagtgg1440aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga atatggagct1500tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta tagccgtcgg1560cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc tggtaaaaga1620ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg taagctccct1680aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg1740tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc1800tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta1860ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc1920agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc1980tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag2040taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca2100gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc2160accatgcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc2220gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta2280ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt2340
tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg2400gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa2460gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg2520atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc2580ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac2640tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg2700atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc2760aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg2820ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt2880atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg2940ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc3000aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc3060gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt3120gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa3180tagagtagat gccgaccgcg ggatcgatcc acttaacgtt actgaaatca tcaaacagct3240tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg3300tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg3360atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata3420cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg3480gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga3540tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat3600atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat3660
gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact3720ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa3780gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac3840gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca3900cttgtttaga ggtaatcctt ctttctagct agaagtcctc gtgtactgtg taagcgccca3960ctccacatct ccactcgacc tgcaggcatg caaagcttga gattaaaata gataaggaaa4020agaaagtgaa aagaaattcg gaagcatggc acattcttct ttttataaat acatgcctga4080ctttcttttt ccatcgatat gatatatgca tatgatagat atacaagcaa tcttcttcaa4140ggagtttgaa attttgtcct ccaggagcaa aaaaaagttt ttttttatac atgtttgtac4200acaagaatag ttaccaattt gctttggtct tacgtgctgc aagtttatat cgttttcaat4260ttctttgtct ttacattttc tttgtccttt atctttcctc atttagtctt tgggagaatt4320aggaaaaggg agcggaaagg taagaaatgc ttgcgtattt tactaattcg gcaaacatcc4380aatttggcaa acagcagcct gtgcaacgct ctcgagatga cagtatcttt gattacactc4440taaatctcga tgacccgacc aaaaagagcg aacaaagaaa taatcttgtg cattcgaata4500tgatggaaga ttttttcccc cttattctaa atgttgacat agcgtgtatg ttatataaac4560aaaaagaaat tgtacaaact ttcttttctt ctctttttat tttatctcta tgctgtcgaa4620gctgcagtca atcagcgtca aggcccgccg cgttgaacta gcccgcgaca tcacgcggcc4680caaagtctgc ctgcatgctc agcggtgctc gttagttcgg ctgcgagtgg cagcaccaca4740gacagaggag gcgctgggaa ccgtgcaggc tgccggcgcg ggcgatgagc acagcgccga4800tgtagcactc cagcagcttg accgggctat cgcagagcgt cgtgcccggc gcaaacggga4860gcagctgtca taccaggctg ccgccattgc agcatcaatt ggcgtgtcag gcattgccat4920cttcgccacc tacctgagat ttgccatgca catgaccgtg ggcggcgcag tgccatgggg4980
tgaagtggct ggcactctcc tcttggtggt tggtggcgcg ctcggcatgg agatgtatgc5040ccgctatgca cacaaagcca tctggcatga gtcgcctctg ggctggctgc tgcacaagag5100ccaccacaca cctcgcactg gaccctttga agccaacgac ttgtttgcaa tcatcaatgg5160actgcccgcc atgctcctgt gtacctttgg cttctggctg cccaacgtcc tgggggcggc5220ctgctttgga gcggggctgg gcatcacgct atacggcatg gcatatatgt ttgtacacga5280tggcctggtg cacaggcgct ttcccaccgg gcccatcgct ggcctgccct acatgaagcg5340cctgacagtg gcccaccagc tacaccacag cggcaagtac ggtggcgcgc cctggggtat5400gttcttgggt ccacaggagc tgcagcacat tccaggtgcg gcggaggagg tggagcgact5460ggtcctggaa ctggactggt ccaagcgggc gattgtgact gatagcgaga ctctgggtcg5520atgttatctg cctcaacaat ggcttagaaa agaagaaaca gaacaaatac agcaaggcaa5580cgcccgtagc ctaggtgatc aaagactgtt gggcttgtct ctgaagcttg taggaaaggc5640agacgctatc atggtgagag ctaagaaggg cattgacaag ttgccggcaa actgtcaagg5700cggtgtacga gctgcttgcc aagtatatgc tgcaattgga tctgtactca agcagcagaa5760gacaacatat cctacaagag ctcatctaaa aggaagcgaa cgtgccaaga ttgctctgtt5820gagtgtatac aacctctatc aatctgaaga caagcctgtg gctctccgtc aagctagaaa5880gattaagagt ttttttgttg attagtgaat ttttgtttta tttatgtctg atagttcaat5940aaagagacaa cacatacaat ataaaatcat tgtctttaaa tgttaattta gtagagtgta6000aagcctgcat tttttttgta cgcataaaca atgaattcac cccgcttctg gtttttaaat6060aattatgtca aactagggaa aattcttttt tttctcttcg ttcttttttt ggcttgttgt6120ggagtcacag gcttgtcttc agattgatag aggttgtata cactcaacag agcaatcttg6180gcacgttcgc ttccttttag atgagctctt gtaggatatg ttgtcttctg ctgcttgagt6240acagatccaa ttgcagcata tacttggcaa gcagctcgta caccgccttg acagtttgcc6300
ggcaacttgt caatgccctt cttagctctc accatgatag cgtctgcctt tcctacaagc6360ttcagagaca agcccaacag tctttgatca cctaggctac gggcgttgcc ttgctgtatt6420tgttctgttt cttcttttct aagccattgt tgaggcagat aacatcgacc caacatcctc6480gagccatact acagcataaa aggatacgtt ttctttaaca gaaatttacc cttttgttat6540cagcacatac aaaaaaaaag aaatttaaga tgagtaggac ttccattctc tcaaaaattt6600tattcaatcc ataaatgaat tatttttgga caaaaaagaa agattatgcc tgattttctc6660tatttttttt ttttttacaa ctccaccaat actttctagc ccagcttggc gtaatcatgg6720tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc6780ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg6840ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc6900ggccaacgcg cggggagagg cggtttgcgt attgggccaa agacaaaagg gcgacattca6960accgattgag ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca7020ccgtcaccga cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta7080ccattagcaa ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta7140gcgacagaat caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg7200gtcatagccc ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag7260ccaccaccgg aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca7320ccaccctcag agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggc7380ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat7440tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct7500cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa7560ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca7620
aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg aactccacga aaatatccga7680acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt7740gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc7800cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc7860gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac7920gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag7980gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca8040agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg8100aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg8160tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca8220ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg8280tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct8340tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg8400cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc8460attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc8520tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc8580acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc8640agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct8700gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg8760aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg8820cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt8880ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta8940
gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt9000agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca9060gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt9120aattctccgc tcatgatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg9180acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat9240ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg9300gttccccaga tctggcgccg gccagcgaga cgagcaagat tggccgccgc ccgaaacgat9360ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac caacgcatac agcgccagca9420gaatgccata gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca9480gcaccggcat aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga9540tcaggggtat gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa9600cgcgcggatt ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt9660gtcaagcatg acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa9720cgaggtcggc gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca9780gccggcgctt tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc9840catgctggcg gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt9900tctgatcggg aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg9960catccatgcc ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg10020cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag10080ctacttcact gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg10140cggcggcacc gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt10200cgacgaagcc ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt10260
ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc10320aggaccgctg ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct10380ccccctttcc accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc10440cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcttcc10500gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct10560cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg10620tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc10680cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga10740aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct10800cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg10860gcgcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc10920atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg10980tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc11040cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg11100ctggccggct accgccggcg taacagatga gggcaagcgg atggctgatg aaaccaagcc11160aaccaggaag ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat11220tgaggaaaag gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca11280gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa11340tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac11400ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga11460cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta11520gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca11580
agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgagc11640gcctttgcga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc11700cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt11760tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact11820tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg11880gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc11940ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc12000gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga12060tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc12120aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca12180atatttataa accttgtttt taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg12240aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc12300ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag cgctggcagt12360ccttgccatt gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc12420cggaagcatt gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag12480tgagggcggc ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga12540cttcatggcg gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc12600cgtgctcgtg ttcgggggtg cgataaaccc agcgaaccat ttgaggtgat aggtaagatt12660ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat12720ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat12780attgacaata ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga12840tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca12900
taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt12960ctatcataat tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc13020gatgactttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc gtcccagccg13080tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct13140gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat ccgtcatcca13200tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg ccatagtgcg13260ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg tcatacgcgt aaaacagcca13320gcgctggcgc gatttagccc cgacatagcc ccactgttcg tccatttccg cgcagacgat13380gacgtcactg cccggctgta tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga13440cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg ttgcccggca tccaacgcca13500ttcatggcca tatcaatgat tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac13560tgcagttgcc atgttttacg gcagtgagag cagagatagc gctgatgtcc ggcggtgctt13620ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat agacacagaa13680gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc13740cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac13800aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg13860gagttcgtct tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa13920ggaaataata aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat13980accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag14040aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg14100tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc14160tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc14220tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg14280agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag14340acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg14400aaaactggga agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga14460cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct14520ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca14580agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt14640atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt14700atattttact ggatgaattg ttttagtacc tagatgtggc gcaacgatgc cggcgacaag14760caggagcgca ccgacttctt ccgcatcaag tgttttggct ctcaggccga ggcccacggc14820aagtatttgg gcaaggggtc gctggtattc gtgcagggca agattcggaa taccaagtac14880gagaaggacg gccagacggt ctacgggacc gacttcattg ccgataaggt ggattatctg14940gacaccaagg caccaggcgg gtcaaatcag gaataagggc acattgcccc ggcgtgagtc15000ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa15060gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc15120atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc15180aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc15240gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc15300gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa15360caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa15420atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac15480gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg15540
caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag15600ctgcgggccg acgatgacga actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc15660cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg15720atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg15780atgggcttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc15840cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc15900gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg15960tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc16020aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc16080gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg16140gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg16200ggttcagcag ccagcgcttt actggcattt caggaacaag cgggcactgc tcgacgcact16260tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta cgaactgccg ataaacagag16320gattaaaatt gacaattgtg attaaggctc agattcgacg gcttggagcg gccgacgtgc16380aggatttccg cgagatccga ttgtcggccc tgaagaaagc tccagagatg ttcgggtccg16440tttacgagca cgaggagaaa aagcccatgg aggcgttcgc tgaacggttg cgagatgccg16500tggcattcgg cgcctacatc gacggcgaga tcattgggct gtcggtcttc aaacaggagg16560acggccccaa ggacgctcac aaggcgcatc tgtccggcgt tttcgtggag cccgaacagc16620gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc ggcgggttta ttgctcgtga16680tgatcgtccg acagattcca acgggaatct ggtggatgcg catcttcatc ctcggcgcac16740ttaatatttc gctattctgg agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg16800tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt gttcatctct gccgctctgc16860
taggtagccc gatacgattg atggcggtcc tgggggctat ttgcggaact gcgggcgtgg16920cgctgttggt gttgacacca aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg16980cgggggcggt ttccatggcg ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc17040ctctgctcac ctttaccgcc tggcaactgg cggccggagg acttctgctc gttccagtag17100ctttagtgtt tgatccgcca atcccgatgc ctacaggaac caatgttctc ggcctggcgt17160ggctcggcct gatcggagcg ggtttaacct acttcctttg gttccggggg atctcgcgac17220tcgaacctac agttgtttcc ttactgggct ttctcagccc cagatctggg gtcgatcagc17280cggggatgca tcaggccgac agtcggaact tcgggtcccc gacctgtacc attcggtgag17340caatggatag gggagttgat atcgtcaacg ttcacttcta aagaaatagc gccactcagc17400ttcctcagcg gctttatcca gcgatttcct attatgtcgg catagttctc aagatcgaca17460gcctgtcacg gttaagcgag aaatgaataa gaaggctgat aattcggatc tctgcgaggg17520agatgatatt tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct17580ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc17640ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact17700gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg17760ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac17820acattgcgga cgtttttaat gtactggggt ggtttttctt ttcaccagtg agacgggcaa17880cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt17940ttgccccagc aggcgaaaat cctgtttgat ggtggttccg aaatcggcaa aatcccttat18000aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca18060ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc18120ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta18180
aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg18240gcgagaaagg aagggaagaa agcgaaagga gcgggcgcca ttcaggctgc gcaactgttg18300ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc18360tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac18420ggccagtgaa ttcgagctcg gtacccggg 18449<210>50<211>18617<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>50ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg 120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac 180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc 240
cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag1560
ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt1920tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa2880
agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga3240cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg4200
gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg4560ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg5520
agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca5880caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg6840
cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag7200cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag8160
acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc8520ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc9480
tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 9840ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt gagattaaaa10800
tagataagga aaagaaagtg aaaagaaatt cggaagcatg gcacattctt ctttttataa10860atacatgcct gactttcttt ttccatcgat atgatatatg catatgatag atatacaagc10920aatcttcttc aaggagtttg aaattttgtc ctccaggagc aaaaaaaagt ttttttttat10980acatgtttgt acacaagaat agttaccaat ttgctttggt cttacgtgct gcaagtttat11040atcgttttca atttctttgt ctttacattt tctttgtcct ttatctttcc tcatttagtc11100tttgggagaa ttaggaaaag ggagcggaaa ggtaagaaat gcttgcgtat tttactaatt11160cggcaaacat ccaatttggc aaacagcagc ctgtgcaacg ctctcgagat gacagtatct11220ttgattacac tctaaatctc gatgacccga ccaaaaagag cgaacaaaga aataatcttg11280tgcattcgaa tatgatggaa gattttttcc cccttattct aaatgttgac atagcgtgta11340tgttatataa acaaaaagaa attgtacaaa ctttcttttc ttctcttttt attttatctc11400tatgctgtcg aagctgcagt caatcagcgt caaggcccgc cgcgttgaac tagcccgcga11460catcacgcgg cccaaagtct gcctgcatgc tcagcggtgc tcgttagttc ggctgcgagt11520ggcagcacca cagacagagg aggcgctggg aaccgtgcag gctgccggcg cgggcgatga11580gcacagcgcc gatgtagcac tccagcagct tgaccgggct atcgcagagc gtcgtgcccg11640gcgcaaacgg gagcagctgt cataccaggc tgccgccatt gcagcatcaa ttggcgtgtc11700aggcattgcc atcttcgcca cctacctgag atttgccatg cacatgaccg tgggcggcgc11760agtgccatgg ggtgaagtgg ctggcactct cctcttggtg gttggtggcg cgctcggcat11820ggagatgtat gcccgctatg cacacaaagc catctggcat gagtcgcctc tgggctggct11880gctgcacaag agccaccaca cacctcgcac tggacccttt gaagccaacg acttgtttgc11940aatcatcaat ggactgcccg ccatgctcct gtgtaccttt ggcttctggc tgcccaacgt12000cctgggggcg gcctgctttg gagcggggct gggcatcacg ctatacggca tggcatatat12060gtttgtacac gatggcctgg tgcacaggcg ctttcccacc gggcccatcg ctggcctgcc12120
ctacatgaag cgcctgacag tggcccacca gctacaccac agcggcaagt acggtggcgc12180gccctggggt atgttcttgg gtccacagga gctgcagcac attccaggtg cggcggagga12240ggtggagcga ctggtcctgg aactggactg gtccaagcgg tagaagcttg agattaaaat12300agataaggaa aagaaagtga aaagaaattc ggaagcatgg cacattcttc tttttataaa12360tacatgcctg actttctttt tccatcgata tgatatatgc atatgataga tatacaagca12420atcttcttca aggagtttga aattttgtcc tccaggagca aaaaaaagtt tttttttata12480catgtttgta cacaagaata gttaccaatt tgctttggtc ttacgtgctg caagtttata12540tcgttttcaa tttctttgtc tttacatttt ctttgtcctt tatctttcct catttagtct12600ttgggagaat taggaaaagg gagcggaaag gtaagaaatg cttgcgtatt ttactaattc12660ggcaaacatc caatttggca aacagcagcc tgtgcaacgc tctcgagatg acagtatctt12720tgattacact ctaaatctcg atgacccgac caaaaagagc gaacaaagaa ataatcttgt12780gcattcgaat atgatggaag attttttccc ccttattcta aatgttgaca tagcgtgtat12840gttatataaa caaaaagaaa ttgtacaaac tttcttttct tctcttttta ttttatctct12900atgatccagt tagaacaacc actcagtcat caagcaaaac tgactccagt actgagaagt12960aaatctcagt ttaaggggct tttcattgct attgtcattg ttagcgcatg ggtcattagc13020ctgagtttat tactttccct tgacatctca aagctaaaat tttggatgtt attgcctgtt13080atactatggc aaacattttt atatacggga ttatttatta catctcatga tgccatgcat13140ggcgtagtat ttccccaaaa caccaagatt aatcatttga ttggaacatt gaccctatcc13200ctttatggtc ttttaccata tcaaaaacta ttgaaaaaac attggttaca ccaccacaat13260ccagcaagct caatagaccc ggattttcac aatggtaaac accaaagttt ctttgcttgg13320tattttcatt ttatgaaagg ttactggagt tgggggcaaa taattgcgtt gactattatt13380tataactttg ctaaatacat actccatatc ccaagtgata atctaactta cttttgggtg13440
ctaccctcgc ttttaagttc attacaatta ttctattttg gtactttttt accccatagt13500gaaccaatag ggggttatgt tcagcctcat tgtgcccaaa caattagccg tcctatttgg13560tggtcattta tcacgtgcta tcattttggc taccacgagg aacatcacga atatcctcat13620atttcttggt ggcagttacc agaaatttac aaagcaaaat agaagcttgg cgtaatcatg13680gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc13740cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc13800gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat13860cggccaacgc gcggggagag gcggtttgcg tattgggcca aagacaaaag ggcgacattc13920aaccgattga gggagggaag gtaaatattg acggaaatta ttcattaaag gtgaattatc13980accgtcaccg acttgagcca tttgggaatt agagccagca aaatcaccag tagcaccatt14040accattagca aggccggaaa cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt14100agcgacagaa tcaagtttgc ctttagcgtc agactgtagc gcgttttcat cggcattttc14160ggtcatagcc cccttattag cgtttgccat cttttcataa tcaaaatcac cggaaccaga14220gccaccaccg gaaccgcctc cctcagagcc gccaccctca gaaccgccac cctcagagcc14280accaccctca gagccgccac cagaaccacc accagagccg ccgccagcat tgacaggagg14340cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata14400ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc14460tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa14520attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc14580aaatgtttga acgatcgggg atcatccggg tctgtggcgg gaactccacg aaaatatccg14640aacgcagcaa gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt14700tgatgtggac gccgggcccg atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg14760
ccgttgctgt cgtaatgata tcggcacctt cgaccgcctg ttccgcagag atcccgtggg14820cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa14880cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca14940ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc15000aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag15060gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat15120gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc15180attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc15240gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc15300ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat15360gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg15420cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc15480ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag15540cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg15600cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc15660tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc15720gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat15780gcgaaacgat ccagatccgg tgcagattat ttggattgag agtgaatatg agactctaat15840tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct15900agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct15960tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc16020agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct16080
taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt16140gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata16200tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag16260ggttccccag atctggcgcc ggccagcgag acgagcaaga ttggccgccg cccgaaacga16320tccgacagcg cgcccagcac aggtgcgcag gcaaattgca ccaacgcata cagcgccagc16380agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc16440agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg16500atcaggggta tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga16560acgcgcggat tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag16620tgtcaagcat gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga16680acgaggtcgg cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc16740agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag16800ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat16860ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc16920gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc16980gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca17040gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc17100gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct17160tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat17220tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gacgattgat17280caggaccgct gccggagcgc aacccactca ctacagcaga gccatgtaga caacatcccc17340tccccctttc caccgcgtca gacgcccgta gcagcccgct acgggctttt tcatgccctg17400
ccctagcgtc caagcctcac ggccgcgctc ggcctctctg gcggccttct ggcgctcttc17460cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc17520tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat17580gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt17640ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg17700aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc17760tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt17820ggcgcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc17880catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt17940gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt18000ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag18060gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc18120caaccaggaa gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga18180ttgaggaaaa ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc18240agggctacaa aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca18300atggcgacct gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca18360cggcgcggtt cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg18420acgagcttgg caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt18480agccgctaaa acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc18540aagaagagcg acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgag18600cgcctttgcg acgctca 18617
<210>51<211>18333<212>DNA<213>人工序列<220>
<223>質(zhì)粒<220>
<221>misc_feature<222>(10264)..(10264)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10472)..(10472)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(10563)..(10563)<223>n為a，c，g，或t<400>51ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag 60aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg120aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac180ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc240cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga300caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat360gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat420tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt480ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg540tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc600
cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc 660tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg 720atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag cattgacgtg 780ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg cggcggcctg 840ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat ggcggggccg 900gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct cgtgttcggg 960ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa1020acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag1080acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata1140agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc1200ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga1260ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgggta1320atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc1380agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc1440agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt1500cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag1560ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc1620gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta1680gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc1740tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga1800ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa1860tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt1920
tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca1980ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact ggagcacctc2040aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat tgtggtttca2100aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg2160ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat2220aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc2280taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga2340tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata2400tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga2460catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca2520tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta2580tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt2640tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga2700attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga2760cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga2820ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa2880agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc2940cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt3000tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga3060attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact3120tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg3180ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga3240
cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag3300gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag3360gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg3420ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg3480aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca3540gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc3600gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta3660tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca3720agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt3780tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg3840ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt3900tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg3960acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga4020tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt4080acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg4140accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg4200gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg4260gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac4320ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc4380gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag4440cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg4500tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg4560
ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc gctcagtatc4620gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa aattgacaat4680tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt tccgcgagat4740ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg agcacgagga4800gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat tcggcgccta4860catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc ccaaggacgc4920tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc gaggggtcgc4980cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg tccgacagat5040tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata tttcgctatt5100ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg cgacggtagg5160cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta gcccgatacg5220attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt tggtgttgac5280accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg cggtttccat5340ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc tcacctttac5400cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag tgtttgatcc5460gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg gcctgatcgg5520agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac ctacagttgt5580ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga tgcatcaggc5640cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg ataggggagt5700tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc agcggcttta5760tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt cacggttaag5820cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga tatttgatca5880
caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt5940gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag6000tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat6060cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga6120tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt6180taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc6240accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga6300aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca aaagaatagc6360ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg6420actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat6480cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag6540ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga6600agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg6660cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt6720tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag6780ctcggtaccc ggggatcttt cgacactgaa atacgtcgag cctgctccgc ttggaagcgg6840cgaggagcct cgtcctgtca caactaccaa catggagtac gataagggcc agttccgcca6900gctcattaag agccagttca tgggcgttgg catgatggcc gtcatgcatc tgtacttcaa6960gtacaccaac gctcttctga tccagtcgat catccgctga aggcgctttc gaatctggtt7020aagatccacg tcttcgggaa gccagcgact ggtgacctcc agcgtccctt taaggctgcc7080aacagctttc tcagccaggg ccagcccaag accgacaagg cctccctcca gaacgccgag7140aagaactgga ggggtggtgt caaggaggag taagctcctt attgaagtcg gaggacggag7200
cggtgtcaag aggatattct tcgactctgt attatagata agatgatgag gaattggagg7260tagcatagct tcatttggat ttgctttcca ggctgagact ctagcttgga gcatagaggg7320tcctttggct ttcaatattc tcaagtatct cgagtttgaa cttattccct gtgaaccttt7380tattcaccaa tgagcattgg aatgaacatg aatctgagga ctgcaatcgc catgaggttt7440tcgaaataca tccggatgtc gaaggcttgg ggcacctgcg ttggttgaat ttagaacgtg7500gcactattga tcatccgata gctctgcaaa gggcgttgca caatgcaagt caaacgttgc7560tagcagttcc aggtggaatg ttatgatgag cattgtatta aatcaggaga tatagcatga7620tctctagtta gctcaccaca aaagtcagac ggcgtaacca aaagtcacac aacacaagct7680gtaaggattt cggcacggct acggaagacg gagaagccac cttcagtgga ctcgagtacc7740atttaattct atttgtgttt gatcgagacc taatacagcc cctacaacga ccatcaaagt7800cgtatagcta ccagtgagga agtggactca aatcgacttc agcaacatct cctggataaa7860ctttaagcct aaactataca gaataagata ggtggagagc ttataccgag ctcccaaatc7920tgtccagatc atggttgacc ggtgcctgga tcttcctata gaatcatcct tattcgttga7980cctagctgat tctggagtga cccagagggt catgacttga gcctaaaatc cgccgcctcc8040accatttgta gaaaaatgtg acgaactcgt gagctctgta cagtgaccgg tgactctttc8100tggcatgcgg agagacggac ggacgcagag agaagggctg agtaataagc cactggccag8160acagctctgg cggctctgag gtgcagtgga tgattattaa tccgggaccg gccgcccctc8220cgccccgaag tggaaaggct ggtgtgcccc tcgttgacca agaatctatt gcatcatcgg8280agaatatgga gcttcatcga atcaccggca gtaagcgaag gagaatgtga agccaggggt8340gtatagccgt cggcgaaata gcatgccatt aacctaggta cagaagtcca attgcttccg8400atctggtaaa agattcacga gatagtacct tctccgaagt aggtagagcg agtacccggc8460gcgtaagctc cctaattggc ccatccggca tctgtagggc gtccaaatat cgtgcctctc8520
ctgctttgcc cggtgtatga aaccggaaag gccgctcagg agctggccag cggcgcagac8580cgggaacaca agctggcagt cgacccatcc ggtgctctgc actcgacctg ctgaggtccc8640tcagtccctg gtaggcagct ttgccccgtc tgtccgcccg gtgtgtcggc ggggttgaca8700aggtcgttgc gtcagtccaa catttgttgc catattttcc tgctctcccc accagctgct8760cttttctttt ctctttcttt tcccatcttc agtatattca tcttcccatc caagaacctt8820tatttcccct aagtaagtac tttgctacat ccatactcca tccttcccat cccttattcc8880tttgaacctt tcagttcgag ctttcccact tcatcgcagc ttgactaaca gctaccccgc8940ttgagcagac atcaccatgc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga9000aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt9060cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt9120ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt9180gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg9240tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga9300ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg9360accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc9420ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc9480tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc9540ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg9600gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc9660gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc9720aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag9780cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt9840
ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg9900gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc9960gagggcaaag gaatagagta gatgccgacc gcgggatcga tccacttaac gttactgaaa10020tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag10080ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga10140gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt ttcgggttta10200cctcttccag atacagctca tctgcaatgc attaatgcat tgactgcaac ctagtaacgc10260cttncaggct ccggcgaaga gaagaatagc ttagcagagc tattttcatt ttcgggagac10320gagatcaagc agatcaacgg tcgtcaagag acctacgaga ctgaggaatc cgctcttggc10380tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg10440atagcttgac tatgaaaatt ccgtcaccag cncctgggtt cgcaaagata attgcatgtt10500tcttccttga actctcaagc ctacaggaca cacattcatc gtaggtataa acctcgaaat10560canttcctac taagatggta tacaatagta accatgcatg gttgcctagt gaatgctccg10620taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg tttacccaga10680atgcacaggt acacttgttt agaggtaatc cttctttcta gctagaagtc ctcgtgtact10740gtgtaagcgc ccactccaca tctccactcg acctgcaggc atgcaagctt gagattaaaa10800tagataagga aaagaaagtg aaaagaaatt cggaagcatg gcacattctt ctttttataa10860atacatgcct gactttcttt ttccatcgat atgatatatg catatgatag atatacaagc10920aatcttcttc aaggagtttg aaattttgtc ctccaggagc aaaaaaaagt ttttttttat10980acatgtttgt acacaagaat agttaccaat ttgctttggt cttacgtgct gcaagtttat11040atcgttttca atttctttgt ctttacattt tctttgtcct ttatctttcc tcatttagtc11100tttgggagaa ttaggaaaag ggagcggaaa ggtaagaaat gcttgcgtat tttactaatt11160
cggcaaacat ccaatttggc aaacagcagc ctgtgcaacg ctctcgagat gacagtatct11220ttgattacac tctaaatctc gatgacccga ccaaaaagag cgaacaaaga aataatcttg11280tgcattcgaa tatgatggaa gattttttcc cccttattct aaatgttgac atagcgtgta11340tgttatataa acaaaaagaa attgtacaaa ctttcttttc ttctcttttt attttatctc11400tatgttgtgg atttggaatg ccctgatcgt tttcgttacc gtgattggca tggaagtgat11460tgctgcactg gcacacaaat acatcatgca cggctggggt tggggatggc atctttcaca11520tcatgaaccg cgtaaaggtg cgtttgaagt taacgatctt tatgccgtgg tttttgctgc11580attatcgatc ctgctgattt atctgggcag tacaggaatg tggccgctcc agtggattgg11640cgcaggtatg acggcgtatg gattactcta ttttatggtg cacgacgggc tggtgcatca11700acgttggcca ttccgctata ttccacgcaa gggctacctc aaacggttgt atatggcgca11760ccgtatgcat cacgccgtca ggggcaaaga aggttgtgtt tcttttggct tcctctatgc11820gccgcccctg tcaaaacttc aggcgacgct ccgggaaaga catggcgcta gagcgggcgc11880tgccagagat gcgcagggcg gggaggatga gcccgcatcc gggaagtaag ggcctgacca11940gaggcggcca gcagcagcgt taatttttcg ggcgtggtcg ttgactgccg ctgatcccaa12000agcttgagat taaaatagat aaggaaaaga aagtgaaaag aaattcggaa gcatggcaca12060ttcttctttt tataaataca tgcctgactt tctttttcca tcgatatgat atatgcatat12120gatagatata caagcaatct tcttcaagga gtttgaaatt ttgtcctcca ggagcaaaaa12180aaagtttttt tttatacatg tttgtacaca agaatagtta ccaatttgct ttggtcttac12240gtgctgcaag tttatatcgt tttcaatttc tttgtcttta cattttcttt gtcctttatc12300tttcctcatt tagtctttgg gagaattagg aaaagggagc ggaaaggtaa gaaatgcttg12360cgtattttac taattcggca aacatccaat ttggcaaaca gcagcctgtg caacgctctc12420gagatgacag tatctttgat tacactctaa atctcgatga cccgaccaaa aagagcgaac12480
aaagaaataa tcttgtgcat tcgaatatga tggaagattt tttccccctt attctaaatg12540ttgacatagc gtgtatgtta tataaacaaa aagaaattgt acaaactttc ttttcttctc12600tttttatttt atctctatga tccagttaga acaaccactc agtcatcaag caaaactgac12660tccagtactg agaagtaaat ctcagtttaa ggggcttttc attgctattg tcattgttag12720cgcatgggtc attagcctga gtttattact ttcccttgac atctcaaagc taaaattttg12780gatgttattg cctgttatac tatggcaaac atttttatat acgggattat ttattacatc12840tcatgatgcc atgcatggcg tagtatttcc ccaaaacacc aagattaatc atttgattgg12900aacattgacc ctatcccttt atggtctttt accatatcaa aaactattga aaaaacattg12960gttacaccac cacaatccag caagctcaat agacccggat tttcacaatg gtaaacacca13020aagtttcttt gcttggtatt ttcattttat gaaaggttac tggagttggg ggcaaataat13080tgcgttgact attatttata actttgctaa atacatactc catatcccaa gtgataatct13140aacttacttt tgggtgctac cctcgctttt aagttcatta caattattct attttggtac13200ttttttaccc catagtgaac caataggggg ttatgttcag cctcattgtg cccaaacaat13260tagccgtcct atttggtggt catttatcac gtgctatcat tttggctacc acgaggaaca13320tcacgaatat cctcatattt cttggtggca gttaccagaa atttacaaag caaaatagaa13380gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc13440cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct13500aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc13560agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggccaaaga13620caaaagggcg acattcaacc gattgaggga gggaaggtaa atattgacgg aaattattca13680ttaaaggtga attatcaccg tcaccgactt gagccatttg ggaattagag ccagcaaaat13740caccagtagc accattacca ttagcaaggc cggaaacgtc accaatgaaa ccatcgatag13800
cagcaccgta atcagtagcg acagaatcaa gtttgccttt agcgtcagac tgtagcgcgt13860tttcatcggc attttcggtc atagccccct tattagcgtt tgccatcttt tcataatcaa13920aatcaccgga accagagcca ccaccggaac cgcctccctc agagccgcca ccctcagaac13980cgccaccctc agagccacca ccctcagagc cgccaccaga accaccacca gagccgccgc14040cagcattgac aggaggcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc14100tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta14160atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta14220acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt14280aagaaacttt attgccaaat gtttgaacga tcggggatca tccgggtctg tggcgggaac14340tccacgaaaa tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag14400tcgccgccga cgccgttgat gtggacgccg ggcccgatca tattgtcgct caggatcgtg14460gcgttgtgct tgtcggccgt tgctgtcgta atgatatcgg caccttcgac cgcctgttcc14520gcagagatcc cgtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag14580ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg14640aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg14700ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga14760taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac14820gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga14880atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca14940cgacgagatc atcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg15000cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag15060tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa15120
gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt15180gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt15240cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc15300gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa15360ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt15420gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc15480catcttgttc aatcatgcga aacgatccag atccggtgca gattatttgg attgagagtg15540aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg15600acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt15660ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca15720acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt15780cataacgtga ctcccttaat tctccgctca tgatcagatt gtcgtttccc gccttcagtt15840taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta15900ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg15960tgcatgccaa ccacagggtt ccccagatct ggcgccggcc agcgagacga gcaagattgg16020ccgccgcccg aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa16080cgcatacagc gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc16140gcgcaggagg cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac16200agtgctcaga attacgatca ggggtatgtt gggtttcacg tctggcctcc ggaccagcct16260ccgctggtcc gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg16320tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc16380gccctggacc tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa16440
cggttggggg ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc16500gacgcactgg ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac16560gacgactggc gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc16620taccgcgatg gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg16680gccgacgcgc agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat16740gcgctgatga caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac16800agcgatgccg gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg16860gccgcgatag acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg16920gtgattgtcg atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga16980aagggtgacg attgatcagg accgctgccg gagcgcaacc cactcactac agcagagcca17040tgtagacaac atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg17100gctttttcat gccctgccct agcgtccaag cctcacggcc gcgctcggcc tctctggcgg17160ccttctggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc17220ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata17280acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg17340cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct17400caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa17460gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc17520tcccttcggg aagcgtggcg cttttccgct gcataaccct gcttcggggt cattatagcg17580attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt17640gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca17700cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg17760
aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg17820gctgatgaaa ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca17880gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac17940ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc18000gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc18060accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag18120atcgaagaga agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca18180gagccatgac ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt18240ccccatgcgc tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga18300gcaaggcaag accgagcgcc tttgcgacgc tca 18333<210>52<211>17<212>DNA<213>人工序列<220>
<223>引物<220>
<221>misc_feature<222>(3)..(3)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(9)..(9)<223>n為a，c，g，或t<400>52gcngarggna thtggta 17
<210>53<211>20<212>DNA<213>人工序列<220>
<223>引物<220>
<221>misc_feature<222>(3)..(3)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(6)..(6)<223>n為a，c，g，或t<400>53tcngcnagra adatrttrtg20<210>54<211>27<212>DNA<213>人工序列<220>
<223>引物<400>54aagtgacacc ggttacacgc ttgtctt27<210>55<211>27<212>DNA<213>人工序列<220>
<223>引物
<400>55gcttatcacc atctgttacc tccttgc27<210>56<211>32<212>DNA<213>人工序列<220>
<223>引物<400>56agagagggat ccttaaatgc gaatatcgtt gc 32<210>57<211>32<212>DNA<213>人工序列<220>
<223>引物<400>57agagagggat ccatgtctga tcaaaagaag ca 32<210>58<211>37<212>DNA<213>人工序列<220>
<223>引物<400>58actttattgg atccttaaat gcgaatatcg ttgctgc 37<210>59<211>38<212>DNA<213>人工序列
<220>
<223>引物<400>59gttccaattg gccacatgaa gagtaagaca ggaaacag 38<210>60<211>38<212>DNA<213>人工序列<220>
<223>引物<400>60cctgtcttac tcttcatgtg gccaattgga accaacac38<210>61<211>38<212>DNA<213>人工序列<220>
<223>引物<400>61ctattttaat catatgtctg atcaaaagaa gcatattg38<210>62<211>16103<212>DNA<213>人工序列<220>
<223>引物<220>
<221>misc_feature<222>(3471)..(3471)
<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3679)..(3679)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(3770)..(3770)<223>n為a，c，g，或t<400>62gatctttcga cactgaaata cgtcgagcct gctccgcttg gaagcggcga ggagcctcgt 60cctgtcacaa ctaccaacat ggagtacgat aagggccagt tccgccagct cattaagagc120cagttcatgg gcgttggcat gatggccgtc atgcatctgt acttcaagta caccaacgct180cttctgatcc agtcgatcat ccgctgaagg cgctttcgaa tctggttaag atccacgtct240tcgggaagcc agcgactggt gacctccagc gtccctttaa ggctgccaac agctttctca300gccagggcca gcccaagacc gacaaggcct ccctccagaa cgccgagaag aactggaggg360gtggtgtcaa ggaggagtaa gctccttatt gaagtcggag gacggagcgg tgtcaagagg420atattcttcg actctgtatt atagataaga tgatgaggaa ttggaggtag catagcttca480tttggatttg ctttccaggc tgagactcta gcttggagca tagagggtcc tttggctttc540aatattctca agtatctcga gtttgaactt attccctgtg aaccttttat tcaccaatga600gcattggaat gaacatgaat ctgaggactg caatcgccat gaggttttcg aaatacatcc660ggatgtcgaa ggcttggggc acctgcgttg gttgaattta gaacgtggca ctattgatca720tccgatagct ctgcaaaggg cgttgcacaa tgcaagtcaa acgttgctag cagttccagg780tggaatgtta tgatgagcat tgtattaaat caggagatat agcatgatct ctagttagct840caccacaaaa gtcagacggc gtaaccaaaa gtcacacaac acaagctgta aggatttcgg900cacggctacg gaagacggag aagccacctt cagtggactc gagtaccatt taattctatt960
tgtgtttgat cgagacctaa tacagcccct acaacgacca tcaaagtcgt atagctacca1020gtgaggaagt ggactcaaat cgacttcagc aacatctcct ggataaactt taagcctaaa1080ctatacagaa taagataggt ggagagctta taccgagctc ccaaatctgt ccagatcatg1140gttgaccggt gcctggatct tcctatagaa tcatccttat tcgttgacct agctgattct1200ggagtgaccc agagggtcat gacttgagcc taaaatccgc cgcctccacc atttgtagaa1260aaatgtgacg aactcgtgag ctctgtacag tgaccggtga ctctttctgg catgcggaga1320gacggacgga cgcagagaga agggctgagt aataagccac tggccagaca gctctggcgg1380ctctgaggtg cagtggatga ttattaatcc gggaccggcc gcccctccgc cccgaagtgg1440aaaggctggt gtgcccctcg ttgaccaaga atctattgca tcatcggaga atatggagct1500tcatcgaatc accggcagta agcgaaggag aatgtgaagc caggggtgta tagccgtcgg1560cgaaatagca tgccattaac ctaggtacag aagtccaatt gcttccgatc tggtaaaaga1620ttcacgagat agtaccttct ccgaagtagg tagagcgagt acccggcgcg taagctccct1680aattggccca tccggcatct gtagggcgtc caaatatcgt gcctctcctg ctttgcccgg1740tgtatgaaac cggaaaggcc gctcaggagc tggccagcgg cgcagaccgg gaacacaagc1800tggcagtcga cccatccggt gctctgcact cgacctgctg aggtccctca gtccctggta1860ggcagctttg ccccgtctgt ccgcccggtg tgtcggcggg gttgacaagg tcgttgcgtc1920agtccaacat ttgttgccat attttcctgc tctccccacc agctgctctt ttcttttctc1980tttcttttcc catcttcagt atattcatct tcccatccaa gaacctttat ttcccctaag2040taagtacttt gctacatcca tactccatcc ttcccatccc ttattccttt gaacctttca2100gttcgagctt tcccacttca tcgcagcttg actaacagct accccgcttg agcagacatc2160accatgcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc2220gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta2280
ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt2340tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg2400gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa2460gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg2520atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc2580ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac2640tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg2700atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc2760aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg2820ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt2880atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg2940ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc3000aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc3060gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt3120gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa3180tagagtagat gccgaccgcg ggatcgatcc acttaacgtt actgaaatca tcaaacagct3240tgacgaatct ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg3300tgttcaggat ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg3360atttaatagc tccatgtcaa caagaataaa acgcgttttc gggtttacct cttccagata3420cagctcatct gcaatgcatt aatgcattga ctgcaaccta gtaacgcctt ncaggctccg3480gcgaagagaa gaatagctta gcagagctat tttcattttc gggagacgag atcaagcaga3540tcaacggtcg tcaagagacc tacgagactg aggaatccgc tcttggctcc acgcgactat3600
atatttgtct ctaattgtac tttgacatgc tcctcttctt tactctgata gcttgactat3660gaaaattccg tcaccagcnc ctgggttcgc aaagataatt gcatgtttct tccttgaact3720ctcaagccta caggacacac attcatcgta ggtataaacc tcgaaatcan ttcctactaa3780gatggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac3840gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca3900cttgtttaga ggtaatcctt ctttctagct agaagtcctc gtgtactgtg taagcgccca3960ctccacatct ccactcgacc tgcaggcatg caagcttgag tctatcgcct ccaaaaagta4020cggtgctgaa ttcagatatc aatcgcctgt tgctaaaatt aacactgtcg ataaagacaa4080gcgtgtaacc ggtgtcactt tggaaagcgg agaagtcatt gaagccgatg cagtcgtatg4140taatgcggat cttgtttatg cttatcacca tctgttacct ccttgcaatt ggacaaagaa4200gacattagcc tcaaagaaac tcacttcatc atctatttcg ttttattggt ccatgtcaac4260aaaggtgcct caattagacg tacacaatat cttcttggct gaagcctaca aggaaagttt4320tgatgagatt ttcaacgact tcggtttgcc ctctgaagct tggcgtaatc atggtcatag4380ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc4440ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc4500tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa4560cgcgcgggga gaggcggttt gcgtattggg ccaaagacaa aagggcgaca ttcaaccgat4620tgagggaggg aaggtaaata ttgacggaaa ttattcatta aaggtgaatt atcaccgtca4680ccgacttgag ccatttggga attagagcca gcaaaatcac cagtagcacc attaccatta4740gcaaggccgg aaacgtcacc aatgaaacca tcgatagcag caccgtaatc agtagcgaca4800gaatcaagtt tgcctttagc gtcagactgt agcgcgtttt catcggcatt ttcggtcata4860gcccccttat tagcgtttgc catcttttca taatcaaaat caccggaacc agagccacca4920
ccggaaccgc ctccctcaga gccgccaccc tcagaaccgc caccctcaga gccaccaccc4980tcagagccgc caccagaacc accaccagag ccgccgccag cattgacagg aggcccgatc5040tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt5100ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa5160taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat5220gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt5280tgaacgatcg gggatcatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag5340caagatatcg cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg5400gacgccgggc ccgatcatat tgtcgctcag gatcgtggcg ttgtgcttgt cggccgttgc5460tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca gagatcccgt gggcgaagaa5520ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc5580gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg5640cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg5700cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg5760tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga5820tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc5880accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcatc gccgtcgggc5940atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc6000agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt6060ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca6120tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc6180ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct6240
gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca6300ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc6360cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc6420ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac6480gatccagatc cggtgcagat tatttggatt gagagtgaat atgagactct aattggatac6540cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat6600agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca6660ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca6720aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct6780ccgctcatga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga6840tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa6900gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc6960cagatctggc gccggccagc gagacgagca agattggccg ccgcccgaaa cgatccgaca7020gcgcgcccag cacaggtgcg caggcaaatt gcaccaacgc atacagcgcc agcagaatgc7080catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc ggcagcaccg7140gcataatcag gccgatgccg acagcgtcga gcgcgacagt gctcagaatt acgatcaggg7200gtatgttggg tttcacgtct ggcctccgga ccagcctccg ctggtccgat tgaacgcgcg7260gattctttat cactgataag ttggtggaca tattatgttt atcagtgata aagtgtcaag7320catgacaaag ttgcagccga atacagtgat ccgtgccgcc ctggacctgt tgaacgaggt7380cggcgtagac ggtctgacga cacgcaaact ggcggaacgg ttgggggttc agcagccggc7440gctttactgg cacttcagga acaagcgggc gctgctcgac gcactggccg aagccatgct7500ggcggagaat catacgcatt cggtgccgag agccgacgac gactggcgct catttctgat7560
cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg cgcgcatcca7620tgccggcacg cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc ttcgcttcct7680ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa tcagctactt7740cactgttggg gccgtgcttg aggagcaggc cggcgacagc gatgccggcg agcgcggcgg7800caccgttgaa caggctccgc tctcgccgct gttgcgggcc gcgatagacg ccttcgacga7860agccggtccg gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg gattggcgaa7920aaggaggctc gttgtcagga acgttgaagg accgagaaag ggtgacgatt gatcaggacc7980gctgccggag cgcaacccac tcactacagc agagccatgt agacaacatc ccctccccct8040ttccaccgcg tcagacgccc gtagcagccc gctacgggct ttttcatgcc ctgccctagc8100gtccaagcct cacggccgcg ctcggcctct ctggcggcct tctggcgctc ttccgcttcc8160tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca8220aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca8280aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg8340ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg8400acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt8460ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt8520ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt8580tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca8640acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt8700cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc8760ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag8820gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga8880
aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta 8940caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga 9000cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg 9060gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct 9120tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct 9180aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga 9240gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc gagcgccttt 9300gcgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca 9360aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga 9420tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg 9480gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg 9540tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag 9600atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact 9660actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg 9720gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt 9780ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt 9840ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg 9900ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg 9960gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc10020cattgccggg atcggggcag taacgggatg ggcgatcagc ccgagcgcga cgcccggaag10080cattgacgtg ccgcaggtgc tggcatcgac attcagcgac caggtgccgg gcagtgaggg10140cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc ggggcattca cggacttcat10200
ggcggggccg gcaattttta ccttgggcat tcttggcata gtggtcgcgg gtgccgtgct10260cgtgttcggg ggtgcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg10320aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa10380agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac10440aatactgata agataatata tcttttatat agaagatatc gccgtatgta aggatttcag10500ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa10560cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca10620taattgggta atgactccaa cttattgata gtgttttatg ttcagataat gcccgatgac10680tttgtcatgc agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag10740gtgctgcctc agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac10800gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac10860cacgtcaaag ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc10920gaatacgtgc gcaacaaccg tcttccggag actgtcatac gcgtaaaaca gccagcgctg10980gcgcgattta gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc11040actgcccggc tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa11100atcgtgttga ggccaacgcc cataatgcgg gctgttgccc ggcatccaac gccattcatg11160gccatatcaa tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt11220tgccatgttt tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg11280ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagacac agaagccact11340ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccataat11400tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt11460gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc11520
gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat11580aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct11640gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg11700aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac11760gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact11820ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct11880cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca11940tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc12000gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact12060gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa12120agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga12180aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt12240atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg12300agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt12360tactggatga attgttttag tacctagatg tggcgcaacg atgccggcga caagcaggag12420cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat12480ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag12540gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc12600aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca12660atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg12720atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt12780gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc12840
gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag12900cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg12960cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc13020agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag13080ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg13140gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac13200aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg13260gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc13320ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat13380ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc13440ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc13500ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg13560ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg13620acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg13680gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag13740gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat13800gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca13860gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc13920gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa13980aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt14040tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg14100agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat14160
tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc14220ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc14280gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg14340tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata14400tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg14460cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta14520gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt14580tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg14640cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc14700tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag14760tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg14820gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac14880ctacagttgt ttccttactg ggctttctca gccccagatc tggggtcgat cagccgggga14940tgcatcaggc cgacagtcgg aacttcgggt ccccgacctg taccattcgg tgagcaatgg15000ataggggagt tgatatcgtc aacgttcact tctaaagaaa tagcgccact cagcttcctc15060agcggcttta tccagcgatt tcctattatg tcggcatagt tctcaagatc gacagcctgt15120cacggttaag cgagaaatga ataagaaggc tgataattcg gatctctgcg agggagatga15180tatttgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga15240gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac15300atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg15360ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct15420ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg15480
cggacgtttt taatgtactg gggtggtttt tcttttcacc agtgagacgg gcaacagctg15540attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc15600cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca15660aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta15720aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta15780cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg15840aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga15900aaggaaggga agaaagcgaa aggagcgggc gccattcagg ctgcgcaact gttgggaagg15960gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag16020gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag16080tgaattcgag ctcggtaccc ggg16103<210>63<211>25<212>DNA<213>人工序列<220>
<223>引物<400>63ggcgtacttg aaggaaccct taccg 25<210>64<211>25<212>DNA<213>人工序列<220>
<223>引物
<400>64attgatgctc ccggtcaccg tgatt 25<210>65<211>500<212>DNA<213>三孢布拉霉(Blakeslea trispora)<400>65aatctataca atgctccata gactcacatt gatattgtcg aagatttcga tgctgactta 60gtagagcaac tacaaaagtt agcagagaag catgatttct taatctttga agaccgcaag120tttgcagata tcggtatgtg aattctatct attttttttc tgatgtgtgc atggatgact180catgatcata ttcttaggta atactgtcaa gcatcaatat ggcaagggcg tttacaagat240tgcttcttgg tctcatatta ctaatgctca cacagttcct ggagaaggta ttatcaaggg300acttgccgaa gtcggcctcc ctcttggtcg tggcttgctt ttgctagcag aaatgtcatc360tcaaggtgca ttaactaagg gtatttacac tgccgaatct gtcaatatgg ctcgccgcaa420caaagatttc gtttttggct ttattgcaca acacaaaatg aatcagtatg atgatgagga480ttttgttgtc atgtcgcctg500<210>66<211>611<212>DNA<213>三孢布拉霉<400>66gagattaaaa tagataagga aaagaaagtg aaaagaaatt cggaagcatg gcacattctt 60ctttttataa atacatgcct gactttcttt ttccatcgat atgatatatg catatgatag120atatacaagc aatcttcttc aaggagtttg aaattttgtc ctccaggagc aaaaaaaagt180ttttttttat acatgtttgt acacaagaat agttaccaat ttgctttggt cttacgtgct240gcaagtttat atcgttttca atttctttgt ctttacattt tctttgtcct ttatctttcc300
tcatttagtc tttgggagaa ttaggaaaag ggagcggaaa ggtaagaaat gcttgcgtat360tttactaatt cggcaaacat ccaatttggc aaacagcagc ctgtgcaacg ctctcgagat420gacagtatct ttgattacac tctaaatctc gatgacccga ccaaaaagag cgaacaaaga480aataatcttg tgcattcgaa tatgatggaa gattttttcc cccttattct aaatgttgac540atagcgtgta tgttatataa acaaaaagaa attgtacaaa ctttcttttc ttctcttttt600attttatctc t 611<210>67<211>720<212>DNA<213>三孢布拉霉<400>67atgtcaatac tcacttatct ggaatttcat ctctactata cactacctgt ccttgcggca 60ttgtgttggc tgctaaagcc gtttcactca cagcaagaca atctcaagta taaattttta120atgttgatgg ccgcctctac cgcatcgatt tgggacaatt atatcgttta tcatcgcgct180tggtggtact gtcctacttg tgttgtggct gtcattggct atgtacctct agaagaatac240atgttcttta tcatcatgac tttaatgact gtcgcgttct caaactttgt tatgcgttgg300cacttgcata ctttctttat tagacccaac acttcttgga agcaaacact attagtacgc360cttgtgcctg tttcagcttt attggcaatc acttatcatg cttggcactt gacactgcca420aataaacctt cattttatgg ttcatgcatc ctttggtatg cttgtcctgt gttggctatt480ctttggctgg gtgctggcga atatatcttg cgtcgacctg tggctgtcct tttgtctatt540gttatcccta gtgtatacct atgttgggct gatatcgtcg ctattagtgc tggcacatgg600catatttctc ttagaacaag cactggcaaa atggtagtac ccgatttacc tgtagaagaa660tgcctgtttt ttactttgat caacacagtc ttggtttttg ctacctgtgc tatagaccgc720
<210>68<211>1089<212>DNA<213>三孢布拉霉<400>68ctgtacaaat catctgttca aaatcaaaac cctaaacaag ccatttccct tttccagcat 60gtcaaagagc tagcatgggc cttctgtctt cctgaccaaa tgctcaacaa tgaattgttt 120gatgatctta ctatcagctg ggatatttta cgtaaagcct caaagtcatt ctatactgca 180tctgccgttt ttccaagtta tgtacgtcaa gacttgggtg ttctctatgc tttctgcaga 240gctaccgatg acctgtgcga tgatgaatcc aaatctgttc aagaaagaag agaccaatta 300gatcttactc gacaatttgt tcgtgatctc tttagccaaa agaccagtgc gcctattgtg 360attgattggg aattgtatca aaaccaactt cctgcttctt gtatatcagc ctttagagcc 420tttactcgcc ttcgccatgt ccttgaagta gaccctgtag aagaactatt agatggttac 480aaatgggatc ttgagcgtcg tcctatcctt gatgaacaag acttggaggc atactctgct 540tgtgtggcca gtagtgtggg tgaaatgtgc acacgtgtga ttcttgctca agaccaaaag 600gaaaatgatg cttggataat tgaccgtgca cgtgagatgg ggctggtgct acaatacgtt 660aacattgctc gagacattgt gactgatagc gagactctgg gtcgatgtta tctgcctcaa 720caatggctta gaaaagaaga aacagaacaa atacagcaag gcaacgcccg tagcctaggt 780gatcaaagac tgttgggctt gtctctgaag cttgtaggaa aggcagacgc tatcatggtg 840agagctaaga agggcattga caagttgccg gcaaactgtc aaggcggtgt acgagctgct 900tgccaagtat atgctgcaat tggatctgta ctcaagcagc agaagacaac atatcctaca 960agagctcatc taaaaggaag cgaacgtgcc aagattgctc tgttgagtgt atacaacctc 1020tatcaatctg aagacaagcc tgtggctctc cgtcaagcta gaaagattaa gagttttttt 1080gttgattag 1089
<210>69<211>611<212>DNA<213>三孢布拉霉<400>69agagataaaa taaaaagaga agaaaagaaa gtttgtacaa tttctttttg tttatataac 60atacacgcta tgtcaacatt tagaataagg gggaaaaaat cttccatcat attcgaatgc120acaagattat ttctttgttc gctctttttg gtcgggtcat cgagatttag agtgtaatca180aagatactgt catctcgaga gcgttgcaca ggctgctgtt tgccaaattg gatgtttgcc240gaattagtaa aatacgcaag catttcttac ctttccgctc ccttttccta attctcccaa300agactaaatg aggaaagata aaggacaaag aaaatgtaaa gacaaagaaa ttgaaaacga360tataaacttg cagcacgtaa gaccaaagca aattggtaac tattcttgtg tacaaacatg420tataaaaaaa aacttttttt tgctcctgga ggacaaaatt tcaaactcct tgaagaagat480tgcttgtata tctatcatat gcatatatca tatcgatgga aaaagaaagt caggcatgta540tttataaaaa gaagaatgtg ccatgcttcc gaatttcttt tcactttctt ttccttatct600attttaatct c 611<210>70<211>882<212>DNA<213>雨生紅球藻<400>70atgctgtcga agctgcagtc aatcagcgtc aaggcccgcc gcgttgaact agcccgcgac 60atcacgcggc ccaaagtctg cctgcatgct cagcggtgct cgttagttcg gctgcgagtg120gcagcaccac agacagagga ggcgctggga accgtgcagg ctgccggcgc gggcgatgag180cacagcgccg atgtagcact ccagcagctt gaccgggcta tcgcagagcg tcgtgcccgg240
cgcaaacggg agcagctgtc ataccaggct gccgccattg cagcatcaat tggcgtgtca300ggcattgcca tcttcgccac ctacctgaga tttgccatgc acatgaccgt gggcggcgca360gtgccatggg gtgaagtggc tggcactctc ctcttggtgg ttggtggcgc gctcggcatg420gagatgtatg cccgctatgc acacaaagcc atctggcatg agtcgcctct gggctggctg480ctgcacaaga gccaccacac acctcgcact ggaccctttg aagccaacga cttgtttgca540atcatcaatg gactgcccgc catgctcctg tgtacctttg gcttctggct gcccaacgtc600ctgggggcgg cctgctttgg agcggggctg ggcatcacgc tatacggcat ggcatatatg660tttgtacacg atggcctggt gcacaggcgc tttcccaccg ggcccatcgc tggcctgccc720tacatgaagc gcctgacagt ggcccaccag ctacaccaca gcggcaagta cggtggcgcg780ccctggggta tgttcttggg tccacaggag ctgcagcaca ttccaggtgc ggcggaggag840gtggagcgac tggtcctgga actggactgg tccaagcggt ag 882<210>71<211>528<212>DNA<213>嗜夏孢歐文氏菌<400>71atgttgtgga tttggaatgc cctgatcgtt ttcgttaccg tgattggcat ggaagtgatt 60gctgcactgg cacacaaata catcatgcac ggctggggtt ggggatggca tctttcacat120catgaaccgc gtaaaggtgc gtttgaagtt aacgatcttt atgccgtggt ttttgctgca180ttatcgatcc tgctgattta tctgggcagt acaggaatgt ggccgctcca gtggattggc240gcaggtatga cggcgtatgg attactctat tttatggtgc acgacgggct ggtgcatcaa300cgttggccat tccgctatat tccacgcaag ggctacctca aacggttgta tatggcgcac360cgtatgcatc acgccgtcag gggcaaagaa ggttgtgttt cttttggctt cctctatgcg420
ccgcccctgt caaaacttca ggcgacgctc cgggaaagac atggcgctag agcgggcgct480gccagagatg cgcagggcgg ggaggatgag cccgcatccg ggaagtaa 528<210>72<211>762<212>DNA<213>念珠藻 PCC73102<400>72atgatccagt tagaacaacc actcagtcat caagcaaaac tgactccagt actgagaagt 60aaatctcagt ttaaggggct tttcattgct attgtcattg ttagcgcatg ggtcattagc120ctgagtttat tactttccct tgacatctca aagctaaaat tttggatgtt attgcctgtt180atactatggc aaacattttt atatacggga ttatttatta catctcatga tgccatgcat240ggcgtagtat ttccccaaaa caccaagatt aatcatttga ttggaacatt gaccctatcc300ctttatggtc ttttaccata tcaaaaacta ttgaaaaaac attggttaca ccaccacaat360ccagcaagct caatagaccc ggattttcac aatggtaaac accaaagttt ctttgcttgg420tattttcatt ttatgaaagg ttactggagt tgggggcaaa taattgcgtt gactattatt480tataactttg ctaaatacat actccatatc ccaagtgata atctaactta cttttgggtg540ctaccctcgc ttttaagttc attacaatta ttctattttg gtactttttt accccatagt600gaaccaatag ggggttatgt tcagcctcat tgtgcccaaa caattagccg tcctatttgg660tggtcattta tcacgtgcta tcattttggc taccacgagg aacatcacga atatcctcat720atttcttggt ggcagttacc agaaatttac aaagcaaaat ga 762<210>73<211>617<212>DNA<213>雨生紅球藻<400>73
tagggtgcgg aaccaggcac gctggtttca cacctcatgc ctgtgataag gtgtggctag 60agcgatgcgt gtgagacggg tatgtcacgg tcgactggtc tgatggccaa tggcatcggc120catgtctggt catcacgggc tggttgcctg ggtgaaggtg atgcacatca tcatgtgcgg180ttggaggggc tggcacagtg tgggctgaac tggagcagtt gtccaggctg gcgttgaatc240agtgagggtt tgtgattggc ggttgtgaag caatgactcc gcccatattc tatttgtggg300agctgagatg atggcatgct tgggatgtgc atggatcatg gtagtgcagc aaactatatt360cacctagggc tgttggtagg atcaggtgag gccttgcaca ttgcatgatg tactcgtcat420ggtgtgttgg tgagaggatg gatgtggatg gatgtgtatt ctcagacgta gaccttgact480ggaggcttga tcgagagagt gggccgtatt ctttgagagg ggaggctcgt gccagaaatg540gtgagtggat gactgtgacg ctgtacattg caggcaggtg agatgcactg tctcgattgt600aaaatacatt cagatgc 617<210>74<211>1208<212>DNA<213>雨生紅球藻<400>74attgtgactg atagcgagac tctgggtcga tgttatctgc ctcaacaatg gcttagaaaa 60gaagaaacag aacaaataca gcaaggcaac gcccgtagcc taggtgatca aagactgttg120ggcttgtctc tgaagcttgt aggaaaggca gacgctatca tggtgagagc taagaagggc180attgacaagt tgccggcaaa ctgtcaaggc ggtgtacgag ctgcttgcca agtatatgct240gcaattggat ctgtactcaa gcagcagaag acaacatatc ctacaagagc tcatctaaaa300ggaagcgaac gtgccaagat tgctctgttg agtgtataca acctctatca atctgaagac360aagcctgtgg ctctccgtca agctagaaag attaagagtt tttttgttga ttagtgaatt420tttgttttat ttatgtctga tagttcaata aagagacaac acatacaata taaaatcatt480
gtctttaaat gttaatttag tagagtgtaa agcctgcatt ttttttgtac gcataaacaa 540tgaattcacc ccgcttctgg tttttaaata attatgtcaa actagggaaa attctttttt 600ttctcttcgt tctttttttg gcttgttgtg gagtcacagg cttgtcttca gattgataga 660ggttgtatac actcaacaga gcaatcttgg cacgttcgct tccttttaga tgagctcttg 720taggatatgt tgtcttctgc tgcttgagta cagatccaat tgcagcatat acttggcaag 780cagctcgtac accgccttga cagtttgccg gcaacttgtc aatgcccttc ttagctctca 840ccatgatagc gtctgccttt cctacaagct tcagagacaa gcccaacagt ctttgatcac 900ctaggctacg ggcgttgcct tgctgtattt gttctgtttc ttcttttcta agccattgtt 960gaggcagata acatcgaccc aacatcctcg agccatacta cagcataaaa ggatacgttt1020tctttaacag aaatttaccc ttttgttatc agcacataca aaaaaaaaga aatttaagat1080gagtaggact tccattctct caaaaatttt attcaatcca taaatgaatt atttttggac1140aaaaaagaaa gattatgcct gattttctct attttttttt tttttacaac tccaccaata1200ctttctag 1208<210>75<211>6316<212>DNA<213>三孢布拉霉<220>
<221>misc_feature<222>(2694)..(2694)<223>n為a，c，g，或t<220>
<221>misc_feature<222>(4263)..(4263)<223>n為a，c，g，或t
<400>75aaggatgaag aatccaactc taataaaaat cttatggata tctttgatcg actcaaaaag 60gctttcaatg ctattgctat taaaaaaaaa gagagagaga gaactatgag caaaaggact 120ctatgccaag atggcaaaaa ggcaccagaa acccttagtt tattattgca taatccagtc 180gagctagtac ttctgtagct caagcttaac cgaggatctt ggaatcaact cgtctcgtca 240ctcttgccga tgatcctaga aatggtatct atggatgtta tactaacatt gttatctttc 300aaggcctcga agatgttatt gttgcggtga taaataggct gctatgtact gaagttgctc 360tgtaaaatga atctagttca ctgcctactc agcaaatggt tgtttctaat gtctttaaag 420aaagaaaaaa agatacatat agactaccct tcctttcaag actgtaatcg agaatcggcc 480gatggtttat tacaattaga cgctgggaat aagcaaaagg attcatcttt gtaaataaga 540gactggtgca tatgaaagca aggatcgtat caaggaatag ttttgatcga gcatcaccag 600caaatgctgc taatgttggc ttcttctttg cttcctgaga ttgaatggga tgtgcctaga 660gcattgctat ttttaagtgt atactttaga tttgtgtctt tagatttgtg tcattttatt 720tagtcaagaa agatccccct ttctctatgt atgctaagaa gaaggagcaa gaagtgtatt 780tacaagttgg aatgagattg aaatattgta cataataata ataaaaagaa aggtagatca 840aaaaaaatgt tctgcctatt gtaagaaatc gggaccaaca ggtgcttgat aaccagaagt 900agcttccaat tcaggtagag gctctaggga caaatacaca attatgacag gaattttctt 960gttgacttga acactacaag agaaacgggt cagcacaaaa tccgaaaaaa aaaagaaacg1020gaccattcat gtcttaccta tctagctctt tgtcttcaat tgcatcccat tgctcaacca1080cagatacgct tcccaattga gtatattgat gaagtgttcc ctgcattttt cgcttgacta1140attccactac agtcacagtc ttattaatgt tttgtccttt accagtcagg ataatatgat1200ctttttgctt cttctatcaa aaaaataatt cttgttttga ataaaaaaaa caaatattta1260aagaaactac tttgatgacg gtacctggaa taactcgaga cacacatcta catatgcgtt1320
gattttattg tggctaattc gaacctcatt ttctgctggt gggggctgtt gactttcagt1380tgctgagacg tccttcttgc ttcttttata gtcttccact atgattttaa tcaagaaagt1440aagtcagtga tgattgttac aagctatata tcttgaaaaa gaacagagag gtattattat1500cagatgcaac atggttttct gtatcatttt catttcagtt tctctgttca aaaaaaaaaa1560gaacactttc tctttccact cctcaaattt tttctgctaa actcctcgca aaacatgtat1620ttgctttaaa ctacaagttg caattgtctg atttagcaat ttcaatatgc cttttgtgaa1680tccacccaaa aataaacaag tgcttgagta tacttgggtt cagttcaaaa gaaagcaagc1740tttttttttt ctttcttggg aaagaaaaaa aaatattgtt gagccatcct ttaccagcag1800tatgcgagct acgacatagc tggtctaaca atgactgcaa gcaatagatc gagcttagtc1860tttctattgc ttcyttgttt gatctatgtt cggccttacg ctgacctatc caatactcga1920gataggcaac aagatttcga acagtaatga aataaatttc ggataacagt tgtggatgag1980gaagagaaag cgacttgaac tcgagaaact ttgttgaaat gaaatccgac cttttacgtg2040atcatcatgt attatcctct ttttcttttt tttcgtagtg aattacttac tgattgcgct2100caagtcgcgt ctttataaag aagaaaaaaa aatattagaa ctttcaaaaa atataactga2160aaataaaagt gtggctcgga gagcaaatac cacatccttt gtcttcgctt tggtaacacg2220gttaataagc cactataggt gaataatgat catttctgag aataaagcgc ggcttgaagc2280ttatatccat atcaggattc atattaggca caactcacaa ttgaggttcc agaagtgcca2340attttttttt cctgatagcc tgtccaatta agatcaaaaa ccactgagtt ttctctatat2400attttttttt ttcataattc ttaactcttc ttcctctctc tctctctctc tctctttttg2460gcttgcaaaa aaaatcttta gtaataccaa agaaagcaaa ccttttcctt ttcttatttc2520cttgcttgtt ttttaatttt tgatttctct atgctttaaa tacccatttc tttctttctt2580ctgctattac ctatcttttc attcctctcc cccctctctc tcttggtcta taaacatcat2640
gaagtcctct tttaaaagtt cgcttgacat ttatgctgtt tatatacagc atcntgtgtt2700ttccaagtgg ttcattcttg cttttgttct ttcgattttc ctcaacactt atctactgaa2760cgcttcgaag caacagccca aagtgataat caaaaaggtt attgagcggg tagaagtacc2820aagtagagaa caacctaaat cagtcataaa gccctcctcc aagaaacact cttctcatca2880tcagtctgat gtcattcgcc ctcttgatga agtattgggt ttgctcggaa cacccgaggc2940cttgactgat gaagagatca tctctattgt tcaagctggt aaaatggccc cctatgctct3000tgaaaaggtc ttgggcgatt tagagcgcgc tgtccatatc cgtcgtgctt tgatctcccg3060tgactctcgt acgaaaactt tggaagacag tatgcttccc gtgaaaaact atcattatga3120taaagtcatg ggtgcttgtt gtgaaaatgt cattggttat atgcctattc cagtaggtgt3180cgcaggtaag aagttcaaca agtcgcgata tttgacaagt tgctcatcat tttcgaaaca3240ggtcctttgg tgattgatgg tgattctatt catattccca tggcaactac ggaaggttgt3300ttagttgctt ctactgccag aggttgtaaa gcaatcaatg ctggtggtgg tgccaacaca3360attgttgttg ctgatggtat gactcgaggt ccttgtgtcg aatttcctac aatcactcgc3420gctgctgact gtaaacgatg gattgaacaa gagggtgaag ctatcgtgac cgaggcattc3480aattcaactt ctcgttttgc tcgtgttcgt aaattgaaag ttgctcttgc cggtcgtcta3540gtctacatcc gtttctctac cactacaggt gatgcaatgg gcatgaacat gatctccaag3600ggttgtgaaa aggctttaag caagattgct gagagatatc ctgatatgca gatcatttct3660ctttctggta actattgtac tgacaagaaa cctgctgcta tcaactggat tgaaggacgt3720ggtaaatctg ttgttgctga sgctgtcatc cctggtacgg ttgtcgaaaa ggtattgaag3780acctctgtta gtgctttggt tgagctgaac atctctaaaa acctggttgg ttctgctatg3840gctggctccg tcggtggctt taacgctcat gctgctaata ttctaactgc catttacctt3900gctactggtc aagatcctgc tcaaaatgta sagagttcta actgtattac tttgatgaaa3960
gctgtcaatg gcgaaagaga ccttcatatc tcttgtacaa tgccctgtat tgaagtaggc4020accattggtg gtggtactat tttgcctcct caacaagcca tgttggattt cattggtgtg4080cgtggtcctc accctaccga acctggtgcc aatgcccgwc gccttgctcg tgttatctgt4140gcctctgtga tggctggtga attgtcttta tgtgcagctt tggctgctgg tcatcttgta4200aaggcacaca tggctcataa tcgtaatacc actgctgctg ccgctgttgt tcctgcccct4260aanggcatag ttgatgtctc tacacctcct gctacacctg cagaaaagaa tgatcctatt4320cctggaagtt gtatcaagtc atagaattaa tattatatat atatcatata caaaaaaaag4380aaaaaaaaaa cactacatct atttatattt ctccatgtac acacacacac acacatataa4440aaactcttta ttttccaata ttttgctttt ataaataatc ttatttcatt ctaaataaac4500tgtttttttt tattaatcat caaaccctgc tgagagctgt gcaatatcat ctatgttttc4560atggtttaac tctggtatcg gwcgagcctc ctctgtactt gaagtttgta ggcagttttt4620atttaaggct gctggtcgat catgatcatc akcaaacctg acagcatgaa gttttgactg4680atgagcaatt tcactaaggg cagaatctga actctttcgc ttcctactat tgaccatatt4740gtctttaggt ggaatgagtg aatagcgtct tgtcatatgt aacacagaat caacaatatc4800ctggtgatga aactcggcca aacatagcgc ctttctcccc caacaattat aataatcaaa4860atgagaatga catgtacggt tttcctcgat gacaatatcc aacgtcttgt cataatcctc4920tgtgcgyata ccattcatct tttggaagaa cgcacggtag ctctcacaag ctgtcctcag4980agagttccgt gccatgtttc ccaatgctcc tggcaagtcg aaatgaagtt gtcgaatctg5040gcgatgtatg tctacaatgt cgcctgtttc tttcattaga tcaagcattc gtgtagccca5100aatgatgtct atgttatgat tttctttcat tccagtaata actatagttt ctcggcaaat5160cgaatgastg atggagtaaa ttcatcaaaa gtgcaagtaa tacatacagt gcttgaagaa5220atcttgtgta gcacgcctat attatgtaat ataggatcga ttctcgaaac tcgacataac5280
caccaggctt tagcaagcgt tttatttcat tcatgacaag ctattgttaa ttcytgctta5340ataaaacaaa atgaaaaaaa catacccccc tcmaaactta cttcccactc ttgattggaa5400aaacaggtat agacgtgacg catatgtata taatcaaaac actcatcagg atagggtaaa5460ccattgagca catcgcattg ggtgaagaaa gtattaggag gcttgatggc tgtaggatat5520ataggtgcaa tatcaatacc gtaaaactca gcatttggga attctgtagc catctccaga5580atccaagtac ctgtgccaca agcaacatca agcactttag gtaagggtat acattgttgt5640tcttgttgtt gttgttgaca atcacttgag tctgagtttc gttttgattg ttttaatgac5700aataattctt ttacaggtgc tgagaaatta ccgtcaaata gatacttgta aataaaatgc5760taaaaataaa aacaatagaa aaaaaaattg acgctcattt cattactatg gaaataactg5820caaaatctta ccacttgtac aagtctatct tgctcaatct catcgtttgg cagaatgtat5880ttattgttgt agtattgata tcttctacca ttcatgatat aactgtcgct tctaatgctc5940tgaggtgaag tacttgtagg tgaaggtgga agtgacgcaa ttttgtcaag cttaacagga6000tcctctcggc tacatgtttt ctgcatatca ggaaaatctt gtttatttga aacatcaaca6060gtagatgtgg tgtgatcttt tttgaaaata tcgatgcctt cctttgaaag ccttttgaaa6120ggctctttta acttttttga gtgagagcta cccatgatag cttatgaaga attaaaaaga6180aaaaagcaaa aaaaattaaa aaaaaaaaaa gtagcaaaaa attctgtcgt aattatacaa6240gccaatcaaa atcgaaattc atgcaaggca tagatgttca cgtggatttg atggttgatc6300cttttttttt gcaaga6316<210>76<211>1170<212>DNA<213>嗜熱棲熱菌(Thermus thermophilus)<400>76
atgaagcgcc tttccctgag ggaggcctgg ccctacctga aagacctcca gcaagatccc 60ctcgccgtcc tgctggcgtg gggccgggcc cacccccggc tcttccttcc cctgccccgc120ttccccctgg ccctgatctt tgaccccgag ggggtggagg gggcgctcct cgccgagggg180accaccaagg ccaccttcca gtaccgggcc ctctcccgcc tcacggggag gggcctcctc240accgactggg gggaaagctg gaaggaggcg cgcaaggccc tcaaagaccc cttcctgccg300aagaacgtcc gcggctaccg ggaggccatg gaggaggagg cccgggcctt cttcggggag360tggcgggggg aggagcggga cctggaccac gagatgctcg ccctctccct gcgcctcctc420gggcgggccc tcttcgggaa gcccctctcc ccaagcctcg cggagcacgc ccttaaggcc480ctggaccgga tcatggccca gaccaggagc cccctggccc tcctggacct ggccgccgaa540gcccgcttcc ggaaggaccg gggggccctc taccgcgagg cggaagccct catcgtccac600ccgcccctct cccaccttcc ccgagagcgc gccctgagcg aggccgtgac cctcctggtg660gcgggccacg agacggtggc gagcgccctc acctggtcct ttctcctcct ctcccaccgc720ccggactggc agaagcgggt ggccgagagc gaggaggcgg ccctcgccgc cttccaggag780gccctgaggc tctacccccc cgcctggatc ctcacccgga ggctggaaag gcccctcctc840ctgggagagg accggctccc cccgggcacc accctggtcc tctcccccta cgtgacccag900aggctccact tccccgatgg ggaggccttc cggcccgagc gcttcctgga ggaaaggggg960accccttcgg ggcgctactt cccctttggc ctggggcaga ggctctgcct ggggcgggac1020ttcgccctcc tcgagggccc catcgtcctc agggccttct tccgccgctt ccgcctagac1080cccctcccct tcccccgggt cctcgcccag gtcaccctga ggcccgaagg cgggcttccc1140gcgcggccta gggaggaggt gcgggcgtga 1170<210>77<211>2981<212>DNA
<213>三孢布拉霉<400>77tctagaattc attccattcg aaaggatcaa cataaccaat ttaatgacta ctagctaatg 60gatacaaata tacgcacaaa aaaagaaaga attctatgat caaagagaac acagacacag120agtgatacat ttaaatggtt aagttcttat gatgttaaaa tggtaacttt attattgaat180taaatgcgaa tatcgttgct gctttgtact tggaaaacgt taggtaaaag ttggttaatg240aaagaagcag gagttgtagt atcatctctt gggaagaaat agaaaaagag gaaagtaaca300aagtaacaag caagacaata atagatccaa tggctttcgg tcttacgagt ttgttcagga360gcatacttct tttggctatc ttgtaacttt cttggtaagg gattctggcc aaagctttta420cagacttggt cggaagtaag cttacttcca gcaagaacga taggaacacc agtacctgga480tgtgtactac aaagaaaaga gaaatgagta cgtgcgttat taaaaaaaag aaaaaaagag540ggcaaaagta ttacctagct ccgacaaaga aaagattatc ataacggttt gtggaatcct600tggtactagg tctgaaccag agaacttgga acacatcatg agaaagacca agaatagaac660ctctccaaag gttaaacttg ctttgccaaa cactaggatc attcacttct tcatgttcaa720tcaaattagc aaagttgttt actcccaaac gacgttcgat aacttccaga accatcttgc780gtgcacggtt taccaactca ggataatttt cttcagcact gtttcctgtc ttactcttca840tatggccaat tggaaccaac acaataatgg agtccttgtt gggaggtgcg gcagattcat900caattcgaga tggaacgttg acatagaatg aagcttcaga gggcaaaccg aagtcgttga960aaatctcatc aaaactttcc ttgtaggctt cagccaagaa gatattgtgt acgtctaatt1020gaggcacctt tgttgacatg gaccaataaa acgaaataga tgatgaagtg agtttctttg1080aggctaatgt cttctttgtc caattgcaag gaggtaacag atggtgataa gcataaacaa1140gatccgcatt acatacgact gcatcggctt caatgacttc tccgctttcc aaagtgacac1200cggttacacg cttgtcttta tcgacagtgt taattttagc aacaggcgat tgatatctga1260
attcagcacc gtactttttg gaggcgatag actcaagctt ctgaacaacc atgttgaaac1320caccacgagg ataccagata ccttcagcaa actcggtgta ttgtaacaaa ctgtaaactg1380ctggagcatc ataaggcgac atactatatt ccaaaaatag aaaatagaac aatgaatatc1440aaaattcctt tcacttgccc tttttcacat ttctcttttc ccacccccga ccggtctcac1500tcattttttt ttcatcccac accacgcgtt gtatgtgtac ttaccccata tacattgttt1560gaaaagtaaa agccatacgc attttcttgg tttggaaata tttactggct cggtcataga1620tcttaccaaa caagtgcaag cgaaagattt caggcacata ctgaagacga atcaaatccc1680aaatggtttc aaagttgcgc ttgatagcaa taaatgtacc ttgttcataa tggacatgtg1740tttccttcat gaaatccaag aatctaccaa atccaagggg accctcaata cggtccaatt1800cgcccttcat cttggttaaa tcggaagaga gttgtacggc atcaccgtcg tcaaaatgaa1860ccttatagtt attgtcacag cgaagcaaat ccaaatgatc accaatacgt tcatccaaat1920cagcaaatgc atcttcaaaa agcttaggca tcaaatagag tgagggaccc tgatcaaagc1980gatgaccatc gtgatgaatg aatgaacaac ggccaccgga aaagtcgttc ttttcaacaa2040cagtaactcg aaaaccttca cgagcaagac gagcagcagt agcagttccg ccaataccgg2100caccaatgac aacaatatgc ttcttttgat cagacatgag attaaaatag ataaggaaaa2160gaaagtgaaa agaaattcgg aagcatggca cattcttctt tttataaata catgcctgac2220tttctttttc catcgatatg atatatgcat atgatagata tacaagcaat cttcttcaag2280gagtttgaaa ttttgtcctc caggagcaaa aaaaagtttt tttttataca tgtttgtaca2340caagaatagt taccaatttg ctttggtctt acgtgctgca agtttatatc gttttcaatt2400tctttgtctt tacattttct ttgtccttta tctttcctca tttagtcttt gggagaatta2460ggaaaaggga gcggaaaggt aagaaatgct tgcgtatttt actaattcgg caaacatcca2520atttggcaaa cagcagcctg tgcaacgctc tcgagatgac agtatctttg attacactct2580
aaatctcgat gacccgacca aaaagagcga acaaagaaat aatcttgtgc attcgaatat2640gatggaagat tttttccccc ttattctaaa tgttgacata gcgtgtatgt tatataaaca2700aaaagaaatt gtacaaactt tcttttcttc tctttttatt ttatctctat gtcaatactc2760acttatctgg aatttcatct ctactataca ctacctgtcc ttgcggcatt gtgttggctg2820ctaaagccgt ttcactcaca gcaagacaat ctcaagtata aatttttaat gttgatggcc2880gcctctaccg catcgatttg ggacaattat atcgtttatc atcgcgcttg gtggtactgt2940cctacttgtg ttgtggctgt cattggctat gtacctctag a2981<210>78<211>1749<212>DNA<213>三孢布拉霉<400>78atgtctgatc aaaagaagca tattgttgtc attggtgccg gtattggcgg aactgctact 60gctgctcgtc ttgctcgtga aggttttcga gttactgttg ttgaaaagaa cgacttttcc120ggtggccgtt gttcattcat tcatcacgat ggtcatcgct ttgatcaggg tccctcactc180tatttgatgc ctaagctttt tgaagatgca tttgctgatt tggatgaacg tattggtgat240catttggatt tgcttcgctg tgacaataac tataaggttc attttgacga cggtgatgcc300gtacaactct cttccgattt aaccaagatg aagggcgaat tggaccgtat tgagggtccc360cttggatttg gtagattctt ggatttcatg aaggaaacac atgtccatta tgaacaaggt420acatttattg ctatcaagcg caactttgaa accatttggg atttgattcg tcttcagtat480gtgcctgaaa tctttcgctt gcacttgttt ggtaagatct atgaccgagc cagtaaatat540ttccaaacca agaaaatgcg tatggctttt acttttcaaa caatgtatat gggtatgtcg600ccttatgatg ctccagcagt ttacagtttg ttacaataca ccgagtttgc tgaaggtatc660
tggtatcctc gtggtggttt caacatggtt gttcagaagc ttgagtctat cgcctccaaa 720aagtacggtg ctgaattcag atatcaatcg cctgttgcta aaattaacac tgtcgataaa 780gacaagcgtg taaccggtgt cactttggaa agcggagaag tcattgaagc cgatgcagtc 840gtatgtaatg cggatcttgt ttatgcttat caccatctgt tacctccttg caattggaca 900aagaagacat tagcctcaaa gaaactcact tcatcatcta tttcgtttta ttggtccatg 960tcaacaaagg tgcctcaatt agacgtacac aatatcttct tggctgaagc ctacaaggaa1020agttttgatg agattttcaa cgacttcggt ttgccctctg aagcttcatt ctatgtcaac1080gttccatctc gaattgatga atctgccgca cctcccaaca aggactccat tattgtgttg1140gttccaattg gccatatgaa gagtaagaca ggaaacagtg ctgaagaaaa ttatcctgag1200ttggtaaacc gtgcacgcaa gatggttctg gaagttatcg aacgtcgttt gggagtaaac1260aactttgcta atttgattga acatgaagaa gtgaatgatc ctagtgtttg gcaaagcaag1320tttaaccttt ggagaggttc tattcttggt ctttctcatg atgtgttcca agttctctgg1380ttcagaccta gtaccaagga ttccacaaac cgttatgata atcttttctt tgtcggagct1440agtacacatc caggtactgg tgttcctatc gttcttgctg gaagtaagct tacttccgac1500caagtctgta aaagctttgg ccagaatccc ttaccaagaa agttacaaga tagccaaaag1560aagtatgctc ctgaacaaac tcgtaagacc gaaagccatt ggatctatta ttgtcttgct1620tgttactttg ttactttcct ctttttctat ttcttcccaa gagatgatac tacaactcct1680gcttctttca ttaaccaact tttacctaac gttttccaag tacaaagcag caacgatatt1740cgcatttaa1749<210>79<211>25<212>DNA<213>人工序列
<220>
<223>引物<400>79ccgatggcga cgacggaagg ttgtt 25<210>80<211>25<212>DNA<213>人工序列<220>
<223>引物<400>80catgttcatg cccattgcat cacct 2權(quán)利要求
1.使用經(jīng)遺傳修飾的布拉霉屬生物產(chǎn)生類胡蘿卜素或其前體的方法，該方法包括以下步驟(i)轉(zhuǎn)化至少一個細(xì)胞，(ii)步驟(i)中獲得的細(xì)胞任選地同型核轉(zhuǎn)變以產(chǎn)生這樣的細(xì)胞，其中細(xì)胞核的一個或多個遺傳性狀都經(jīng)同樣方式進(jìn)行了修飾并且所述遺傳修飾在細(xì)胞中得到顯現(xiàn)，以及(iii)篩選和繁殖經(jīng)遺傳修飾的細(xì)胞，(iv)培養(yǎng)經(jīng)遺傳修飾的細(xì)胞，(v)制備由經(jīng)遺傳修飾的細(xì)胞產(chǎn)生的類胡蘿卜素或制備由所述經(jīng)遺傳修飾的細(xì)胞產(chǎn)生的類胡蘿卜素前體。
2.根據(jù)權(quán)利要求1所述的方法，其中細(xì)胞來自三孢布拉霉種的真菌。
3.根據(jù)權(quán)利要求1或2所述的方法，其中在轉(zhuǎn)化(i)中使用載體或游離的核酸。
4.根據(jù)權(quán)利要求3所述的方法，其中在轉(zhuǎn)化(i)中使用的載體整合入至少一個細(xì)胞的基因組中。
5.根據(jù)權(quán)利要求4所述的方法，其中在轉(zhuǎn)化(i)中使用的載體包含啟動子和/或終止子。
6.根據(jù)前述權(quán)利要求3至5中任意一項所述的方法，其中在轉(zhuǎn)化(i)中使用包含gpd、pcarB、pcarRA和/或ptef1啟動子和/或trpC終止子的載體。
7.根據(jù)前述權(quán)利要求3至6中任意一項所述的方法，其中在轉(zhuǎn)化(i)中使用包含抗性基因的載體。
8.根據(jù)權(quán)利要求7所述的方法，其中在轉(zhuǎn)化(i)中使用的載體包含潮霉素抗性基因(hph)，特別是包含來自大腸桿菌的潮霉素抗性基因(hph)。
9.根據(jù)前述權(quán)利要求5至8中任意一項所述的方法，其中g(shù)pd啟動子具有序列SEQ ID NO1。
10.根據(jù)前述權(quán)利要求5至8中任意一項所述的方法，其中trpC終止子具有序列SEQ ID NO2。
11.根據(jù)前述權(quán)利要求5至8中任意一項所述的方法，其中tef1啟動子具有序列SEQ ID NO35。
12.根據(jù)權(quán)利要求6至11中任意一項所述的方法，其中g(shù)pd啟動子和trpC終止子來源于構(gòu)巢曲霉。
13.根據(jù)權(quán)利要求3至12中任意一項所述的方法，其中載體包含SEQID NO3。
14.根據(jù)前述權(quán)利要求中任意一項所述的方法，其中使用農(nóng)桿菌、接合、化學(xué)物質(zhì)、電穿孔、用載有DNA的顆粒轟擊、原生質(zhì)體或顯微注射進(jìn)行轉(zhuǎn)化(i)。
15.根據(jù)前述權(quán)利要求中任意一項所述的方法，其中在同型核轉(zhuǎn)變(ii)中使用誘變劑。
16.根據(jù)權(quán)利要求15所述的方法，其中使用的誘變劑是N-甲基-N’-硝基亞硝基胍(MNNG)、UV輻射或X射線。
17.根據(jù)前述權(quán)利要求中任意一項所述的方法，其中通過標(biāo)記和/或篩選單核細(xì)胞進(jìn)行篩選。
18.根據(jù)前述權(quán)利要求1-17中任意一項所述的方法，其中在篩選中使用5-碳-5-脫氮核黃素(darf)和潮霉素(hyg)或5-氟乳清酸(FOA)和尿嘧啶和潮霉素。
19.根據(jù)權(quán)利要求3至18中任意一項所述的方法，其中轉(zhuǎn)化(i)中使用的載體包括用于產(chǎn)生類胡蘿卜素或其前體的遺傳信息。
20.根據(jù)權(quán)利要求3至19中任意一項所述的方法，其中轉(zhuǎn)化(i)中使用的載體包括用于產(chǎn)生胡蘿卜素或葉黃素的遺傳信息。
21.根據(jù)權(quán)利要求3至20中任意一項所述的方法，其中轉(zhuǎn)化(i)中使用的載體包括用于產(chǎn)生蝦青素、玉米黃素、海膽酮、β-隱黃素、金盞花黃質(zhì)、金盞花玉紅質(zhì)、角黃素、3-羥海膽酮、3’-羥海膽酮、番茄紅素、β-胡蘿卜素、α-胡蘿卜素、葉黃素、六氫番茄紅素、紅木素或八氫番茄紅素。
22.提供至少一種高純度類胡蘿卜素以及含有產(chǎn)類胡蘿卜素生物和至少一種類胡蘿卜素的食品的方法，該方法在培養(yǎng)布拉霉屬產(chǎn)類胡蘿卜素生物后包括以下步驟I)去除生物量，IA)用類胡蘿卜素不可溶的溶劑特別是水任選地洗滌生物量，IB)生物量的滅菌和細(xì)胞破裂，IC)任選地干燥和/或均相分布，并II)從經(jīng)破壞的生物量中通過類胡蘿卜素可溶的溶劑部分提取類胡蘿卜素并從所述生物量中分離所述溶劑，IIA)1)從含有類胡蘿卜素的生物量中去除殘余的溶劑，2)任選地均勻懸浮生物量，并使生物量固體含量＞2％并＜50％，并3)干燥生物量或重懸，用于產(chǎn)生食品，IIB)1)從使用的溶劑中結(jié)晶類胡蘿卜素并特別地通過過濾分離類胡蘿卜素晶體。
23.根據(jù)權(quán)利要求22所述的方法，其中至少一種類胡蘿卜素選自胡蘿卜素和葉黃素。
24.根據(jù)權(quán)利要求22或23所述的方法，其中至少一種類胡蘿卜素選自蝦青素、玉米黃素、海膽酮、β-隱黃素、金盞花黃質(zhì)、金盞花玉紅質(zhì)、角黃素、3-羥海膽酮、3’-羥海膽酮、番茄紅素、β-胡蘿卜素、葉黃素、六氫番茄紅素、紅木素和八氫番茄紅素。
25.根據(jù)權(quán)利要求22至24中任意一項所述的方法，其中至少一種類胡蘿卜素是蝦青素、玉米黃素、紅木素或八氫番茄紅素。
26.根據(jù)權(quán)利要求22至25中任意一項所述的方法，其中使用蒸汽或微波輻射進(jìn)行滅菌和細(xì)胞破裂。
27.根據(jù)權(quán)利要求22-26中任意一項所述的方法，其中用二氯甲烷或超臨界二氧化碳或四氫呋喃從生物量中提取類胡蘿卜素。
28.根據(jù)權(quán)利要求27所述的方法，其中直接分離溶于超臨界二氧化碳中的類胡蘿卜素或置于二氯甲烷中。
29.根據(jù)權(quán)利要求22-28中任意一項所述的方法，其中用一步或根據(jù)需要用多步方法從生物量中提取類胡蘿卜素。
30.根據(jù)權(quán)利要求22-29中任意一項所述的方法，其中在步驟IA1)中用蒸汽蒸餾從生物量中去除溶劑。
31.根據(jù)權(quán)利要求22-30中任意一項所述的方法，其中步驟IIA3)中的干燥用噴霧干燥或接觸干燥實(shí)施。
32.根據(jù)前述權(quán)利要求中任意一項所述的方法，其中步驟IIB1)中的結(jié)晶是通過用類胡蘿卜素不溶的溶劑逐漸替換溶劑而實(shí)施的。
33.根據(jù)權(quán)利要求32所述的方法，其中用水或用低級醇、特別是甲醇替換所使用的溶劑。
34.根據(jù)權(quán)利要求13所述的方法，其中布拉霉屬的經(jīng)遺傳修飾生物可以通過用具有選自SEQ ID NO37-51和62的序列的載體轉(zhuǎn)化而產(chǎn)生。
35.產(chǎn)生含有產(chǎn)類胡蘿卜素生物和至少一種類胡蘿卜素的食品的方法，該方法在培養(yǎng)布拉霉屬產(chǎn)類胡蘿卜素生物后包括以下步驟I)均勻懸浮培養(yǎng)肉湯的固體，并IIA)對于培養(yǎng)肉湯的生物量固體含量＞2％1)任選地將培養(yǎng)肉湯濃縮至固體含量＜50％，并2)將培養(yǎng)肉湯干燥以產(chǎn)生食品，或IIB)對于培養(yǎng)肉湯的固體含量＜2％1)將培養(yǎng)肉湯濃縮至固體含量＞2％且＜50％，并2)將懸液干燥以產(chǎn)生食品，或IIC)不依賴于培養(yǎng)肉湯的固體含量，1)去除生物量，2)任選地用類胡蘿卜素不可溶的溶劑特別是水洗滌生物量，3)滅菌并破壞細(xì)胞，4)任選地干燥和均勻分布，5)用類胡蘿卜素可溶的溶劑從生物量中部分提取類胡蘿卜素，5a)從含有類胡蘿卜素的溶劑中去除含有類胡蘿卜素的生物量，5b)從生物量中去除殘余的溶劑，并5c)干燥生物量以產(chǎn)生食品，6)從5a)使用的溶劑中結(jié)晶類胡蘿卜素并特別地通過過濾分離類胡蘿卜素晶體。
36.根據(jù)權(quán)利要求35所述的方法，其中至少一種類胡蘿卜素選自胡蘿卜素和葉黃素。
37.根據(jù)權(quán)利要求35或36所述的方法，其中至少一種類胡蘿卜素選自蝦青素、玉米黃素、海膽酮、β-隱黃素、金盞花黃質(zhì)、金盞花玉紅質(zhì)、角黃素、3-羥海膽酮、3’-羥海膽酮、番茄紅素、β-胡蘿卜素、葉黃素、紅木素和八氫番茄紅素。
38.根據(jù)權(quán)利要求35-37中任意一項所述的方法，其中至少一種類胡蘿卜素是蝦青素、玉米黃素、紅木素或八氫番茄紅素。
39.根據(jù)權(quán)利要求35-38中任意一項所述的方法，其中步驟113)中用蒸汽或微波輻射進(jìn)行滅菌和細(xì)胞破裂。
40.根據(jù)權(quán)利要求35-39中任意一項所述的方法，其中在步驟IIC5)中用二氯甲烷或超臨界二氧化碳從生物量中提取類胡蘿卜素。
41.根據(jù)權(quán)利要求40中所述的方法，其中直接分離溶于超臨界二氧化碳中的類胡蘿卜素直接或置于二氯甲烷中。
42.根據(jù)權(quán)利要求35-41中任意一項所述的方法，其中用一步或根據(jù)需要用多步方法從生物量中提取類胡蘿卜素。
43.根據(jù)權(quán)利要求35-42中任意一項所述的方法，其中在步驟IIC5b)中用蒸汽蒸餾從生物量中去除溶劑。
44.根據(jù)權(quán)利要求35-43中任意一項所述的方法，其中任一步驟IIA1)、IIB2)和IIC5c)中的干燥是用噴霧干燥或接觸干燥實(shí)施的。
45.根據(jù)權(quán)利要求35-44中任意一項所述的方法，其中步驟IIC6)中的結(jié)晶是通過用類胡蘿卜素不溶的溶劑逐漸替換溶劑而實(shí)施的。
46.根據(jù)權(quán)利要求45中所述的方法，其中用水或用低級醇、特別是甲醇替換使用的溶劑。
47.根據(jù)權(quán)利要求35-46中任意一項所述的方法，其中布拉霉屬的經(jīng)遺傳修飾生物可以通過用具有選自SEQ ID NO37-51和62的序列的載體轉(zhuǎn)化而產(chǎn)生。
48.可通過權(quán)利要求1至47的任意方法產(chǎn)生的食品，特別是動物飼料。
49.可通過權(quán)利要求1至47的任意方法產(chǎn)生的食品添加劑，特別是動物飼料添加劑。
50.根據(jù)權(quán)利要求1-49中任意一項所述的方法，其中食品和動物飼料可通過發(fā)酵獲得。
51.根據(jù)權(quán)利要求1-49中任意一項所述的方法，其中食品添加劑和動物飼料添加劑可通過發(fā)酵獲得。
52.根據(jù)權(quán)利要求1-49中任意一項所述的方法，其中食品、食品添加劑、動物飼料和動物飼料添加劑中至少兩種產(chǎn)品可以通過發(fā)酵獲得。
53.通過權(quán)利要求1至14所述的方法可獲得的類胡蘿卜素的用途，用于生產(chǎn)化妝品、藥物、皮膚病學(xué)制劑、食品、食品添加劑、動物飼料或動物飼料添加劑。
全文摘要
本發(fā)明涉及用經(jīng)遺傳修飾的布拉霉屬生物產(chǎn)生類胡蘿卜素或其前體的方法。所述方法包括以下步驟(i)轉(zhuǎn)化至少一個細(xì)胞，(ii)步驟(i)中獲得的細(xì)胞任選地同型核轉(zhuǎn)變以產(chǎn)生這樣的細(xì)胞，其中細(xì)胞核的一個或多個遺傳性狀都經(jīng)同樣方式進(jìn)行了修飾并且所述遺傳修飾在細(xì)胞中得到顯現(xiàn)，以及(iii)篩選和繁殖經(jīng)遺傳修飾的細(xì)胞，(iv)培養(yǎng)經(jīng)遺傳修飾的細(xì)胞，(v)制備由經(jīng)遺傳修飾的細(xì)胞產(chǎn)生的類胡蘿卜素或由所述經(jīng)遺傳修飾的細(xì)胞產(chǎn)生的類胡蘿卜素前體。本發(fā)明還涉及根據(jù)所述方法產(chǎn)生的類胡蘿卜素或其前體及其用途。
文檔編號A23J1/00GK1759173SQ200480006379
公開日2006年4月12日 申請日期2004年1月9日 優(yōu)先權(quán)日2003年1月9日
發(fā)明者M·馬圖舍克, D·克萊因, T·海內(nèi)坎普, A·施密特, A·布拉克哈格, B·阿哈特茨 申請人:巴斯福股份公司