專利名稱：甘油三磷酸脫氫酶基因與原發(fā)性高血壓的相關(guān)性的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及分子生物學(xué)和醫(yī)學(xué)領(lǐng)域。更具體地涉及人甘油三磷酸脫氫酶基因(glycerol-3-phosphate dehydrogenase 2，GPD2)的單核苷酸多態(tài)性(singlenucleotide polymorphism，SNP)及其與原發(fā)性高血壓的相關(guān)性。本發(fā)明還涉及檢測這些SNP的方法和試劑盒。
背景技術(shù)：
原發(fā)性高血壓(essential hypertension，EH)是最常見的心血管疾病，其發(fā)病率逐年上升，并可引起嚴(yán)重的心、腦、腎并發(fā)癥，是腦卒中和冠心病的主要危險(xiǎn)因素。EH是一種由遺傳因素和環(huán)境因素相互作用而發(fā)病的多基因病，尋找EH相關(guān)基因進(jìn)而闡明高血壓發(fā)病的遺傳機(jī)制已經(jīng)成為目前研究的熱點(diǎn)(HarrapSB.Hypertensiongenes versus environment.Lancet，1994，344169-171)。
定位克隆法是研究EH遺傳機(jī)制的主要研究之一，即利用一定數(shù)量的覆蓋整個(gè)基因組的微衛(wèi)星引物，對大樣本家系或同胞對進(jìn)行連鎖分析，而將EH易感基因位點(diǎn)定位到某一染色體區(qū)域。上海市高血壓研究所已完成對上海地區(qū)漢族高血壓家系的全基因掃描，研究提示2q14-q23區(qū)域與高血壓存在緊密連鎖關(guān)系(Zhu DL，Wang HY，Xiong MM，et al.Linkage of hypertension tochromosome 2q14-q23 in Chinese families.J Hypertens 2001；1955-61)，然而并沒有確定該區(qū)域內(nèi)哪個(gè)基因?yàn)镋H易感基因。GPD2基因即是該定位區(qū)域內(nèi)的眾多候選基因之一，但目前沒有證實(shí)GPD2基因與原發(fā)性高血壓相關(guān)性的報(bào)道，更沒有證實(shí)GPD2基因的SNP與原發(fā)性高血壓相關(guān)性的報(bào)道。
綜上所述，為了最終實(shí)現(xiàn)治療高血壓，本領(lǐng)域迫切需要尋找原發(fā)性高血壓易感基因，并開發(fā)檢測原發(fā)性高血壓的方法、試劑盒，以及相關(guān)的治療藥物。

發(fā)明內(nèi)容
本發(fā)明的目的就是提供一種輔助性診斷(尤其是早期診斷)高血壓等高血壓的方法及檢測試劑盒。
本發(fā)明的另一目的是提供一種新的治療高血壓的方法。
在本發(fā)明的第一方面，提供了一種對個(gè)體的高血壓易感性進(jìn)行輔助性診斷的方法，它包括步驟
檢測該個(gè)體的GPD2基因、轉(zhuǎn)錄本和/或蛋白，并與正常的GPD2基因、轉(zhuǎn)錄本和/或蛋白相比較，存在差異就表明該個(gè)體患高血壓的可能性高于正常人群。
在另一優(yōu)選例中，檢測的是GPD2的基因或轉(zhuǎn)錄本，并與正常GPD2核苷酸序列比較差異。
在另一優(yōu)選例中，所述的差異是選自下組的單核苷酸多態(tài)性115330位A→G；135053位G→A；其中，核苷酸位置編號基于SEQ ID NO1。
在本發(fā)明的第二方面，提供了一種檢測樣品是否存在GPD2基因的單核苷酸多態(tài)性的方法，包括步驟(a)用GPD2基因特異性引物擴(kuò)增樣品的GPD2基因，得到擴(kuò)增產(chǎn)物；和(b)檢測擴(kuò)增產(chǎn)物中是否存在選自下組的單核苷酸多態(tài)性115330位A→G；135053位G→A；其中，核苷酸位置編號基于SEQ ID NO1。
在另一優(yōu)選例中，所述的單核苷酸多態(tài)性是115330位AG。
在本發(fā)明的第三方面，提供了一種藥物組合物，它含有安全有效量的GPD2蛋白以及藥學(xué)上可接受的載體。
還提供了一種GPD2基因和蛋白的用途，它們被用于(a)制備輔助性檢測的試劑；或(b)制備治療高血壓的藥物。
在本發(fā)明的第四方面，提供了一種用于輔助性檢測高血壓的試劑盒，它包括特異性擴(kuò)增GPD2基因或轉(zhuǎn)錄本的引物和說明書。
在另一優(yōu)選例中，所述的試劑盒還含有選自下組的試劑(a)與突變部位結(jié)合的探針；(b)識別突變位點(diǎn)的限制性內(nèi)切酶。
在另一優(yōu)選例中，所述的突變選自115330位A→G；135053位G→A；其中，核苷酸位置編號基于SEQ ID NO1。
更佳地，所述的單核苷酸多態(tài)性是115330位AG。


圖1顯示了GPD2的一種SNP圖像(以SNP04為例)的測序結(jié)果。
具體實(shí)施例方式
本發(fā)明人經(jīng)過多年研究，首次發(fā)現(xiàn)和證明了GPD2基因與高血壓密切相關(guān)，而且發(fā)現(xiàn)了它的新功能GPD2的改變將導(dǎo)致高血壓，其中關(guān)聯(lián)研究結(jié)果顯示，在GPD2第115330位的SNP04(A/G)在病例和對照組中的分布存在顯著性差異(P＜0.05)，該SNP多態(tài)可改變氨基酸編碼進(jìn)而可能影響基因表達(dá)產(chǎn)物的功能，因此與高血壓發(fā)病存在密切的相關(guān)性?；诖税l(fā)現(xiàn)，本發(fā)明提供了利用檢測GPD2基因、轉(zhuǎn)錄本或蛋白是否存在突變，從而為診斷高血壓提供輔助性或早期參考指標(biāo)的方法和試劑盒。
GPD2基因的總長度為149000bp。其詳細(xì)序列可參見網(wǎng)址http://genome.ucsc.edu/或http://www.ncbi.nlm.nih.gov/，或參見SEQ IDNO1。
基于本發(fā)明的新發(fā)現(xiàn)，GPD2蛋白或多肽有多方面的新用途。這些用途包括(但不限于)直接做為藥物治療GPD2蛋白功能低下或喪失所致的疾病(如高血壓)，和用于篩選促進(jìn)GPD2蛋白功能的物質(zhì)，如抗體、多肽或其它配體。用表達(dá)的重組人GPD2蛋白篩選多肽庫可用于尋找有治療價(jià)值的能刺激人GPD2蛋白功能的多肽分子。
另一方面，本發(fā)明還包括對人GPD2 DNA或是其片段編碼的多肽具有特異性的多克隆抗體和單克隆抗體，尤其是單克隆抗體。這里，“特異性”是指抗體能結(jié)合于人GPD2基因產(chǎn)物或片段。較佳地，指那些能與人GPD2基因產(chǎn)物或片段結(jié)合但不識別和結(jié)合于其它非相關(guān)抗原分子的抗體。本發(fā)明中抗體包括那些能夠結(jié)合并抑制人GPD2蛋白的分子，也包括那些并不影響人GPD2蛋白功能的抗體。
本發(fā)明不僅包括完整的單克隆或多克隆抗體，而且還包括具有免疫活性的抗體片段，如Fab’或(Fab)2片段；抗體重鏈；抗體輕鏈；遺傳工程改造的單鏈Fv分子；或嵌合抗體。
本發(fā)明的抗體可以通過本領(lǐng)域內(nèi)技術(shù)人員已知的各種技術(shù)進(jìn)行制備。例如，純化的人GPD2基因產(chǎn)物或者其具有抗原性的片段，可被施用于動(dòng)物以誘導(dǎo)多克隆抗體的產(chǎn)生。與之相似的，表達(dá)人GPD2蛋白或其具有抗原性的片段的細(xì)胞可用來免疫動(dòng)物來生產(chǎn)抗體。多種佐劑可用于增強(qiáng)免疫反應(yīng)，包括但不限于弗氏佐劑等。
本發(fā)明的抗體也可以是單克隆抗體。此類單克隆抗體可以利用雜交瘤技術(shù)來制備。本發(fā)明的抗體包括能阻斷人GPD2蛋白功能的抗體以及不影響人GPD2蛋白功能的抗體。本發(fā)明的各類抗體可以利用人GPD2基因產(chǎn)物的片段或功能區(qū)，通過常規(guī)免疫技術(shù)獲得。這些片段或功能區(qū)可以利用重組方法制備或利用多肽合成儀合成。與人GPD2基因產(chǎn)物的未修飾形式結(jié)合的抗體可以用原核細(xì)胞(例如E.Coli)中生產(chǎn)的基因產(chǎn)物來免疫動(dòng)物而產(chǎn)生；與翻譯后修飾形式結(jié)合的抗體(如糖基化或磷酸化的蛋白或多肽)，可以用真核細(xì)胞(例如酵母或昆蟲細(xì)胞)中產(chǎn)生的基因產(chǎn)物來免疫動(dòng)物而獲得。
抗人GPD2蛋白的抗體可用于免疫組織化學(xué)技術(shù)中，檢測活檢標(biāo)本中的人GPD2蛋白。一種優(yōu)選的抗GPD2抗體是不識別正常GPD2但識別突變GPD2的抗體，或者識別正常GPD2但不識別突變GPD2的抗體。利用該抗體對正常和異常GPD2蛋白的特異性差異，可以方便地進(jìn)行蛋白質(zhì)水平的高血壓易感性檢測。
利用本發(fā)明GPD2蛋白，通過各種常規(guī)篩選方法，可篩選出與GPD2蛋白發(fā)生相互作用的物質(zhì)，如抑制劑、激動(dòng)劑或拮抗劑等。
本發(fā)明GPD2蛋白及其抗體、抑制劑、激動(dòng)劑、拮抗劑等，當(dāng)在治療上進(jìn)行施用(給藥)時(shí)，可提供不同的效果。通常，可將這些物質(zhì)配制于無毒的、惰性的和藥學(xué)上可接受的水性載體介質(zhì)中，其中pH通常約為5-8，較佳地pH約為6-8，盡管pH值可隨被配制物質(zhì)的性質(zhì)以及待治療的病癥而有所變化。配制好的藥物組合物可以通過常規(guī)途徑進(jìn)行給藥，其中包括(但并不限于)肌內(nèi)、靜脈內(nèi)、或皮下給藥。
正常的GPD2多肽可直接用于疾病治療，例如，用于高血壓治療。在使用本發(fā)明GPD2蛋白時(shí)，還可同時(shí)使用其他治療高血壓的藥劑。
本發(fā)明還提供了一種藥物組合物，它含有安全有效量的本發(fā)明GPD2蛋白以及藥學(xué)上可接受的載體或賦形劑。這類載體包括(但并不限于)鹽水、緩沖液、葡萄糖、水、甘油、乙醇、及其組合。藥物制劑應(yīng)與給藥方式相匹配。本發(fā)明的藥物組合物可以被制成針劑形式，例如用生理鹽水或含有葡萄糖和其他輔劑的水溶液通過常規(guī)方法進(jìn)行制備。諸如片劑和膠囊之類的藥物組合物，可通過常規(guī)方法進(jìn)行制備。藥物組合物如針劑、溶液、片劑和膠囊宜在無菌條件下制造?；钚猿煞值慕o藥量是治療有效量，例如每天約0.1微克/千克體重-約10毫克/千克體重。此外，本發(fā)明的多肽還可與其他治療劑一起使用。
使用藥物組合物時(shí)，是將安全有效量的GPD2蛋白或其拮抗劑、激動(dòng)劑施用于哺乳動(dòng)物，其中該安全有效量通常至少約0.1微克/千克體重，而且在大多數(shù)情況下不超過約10毫克/千克體重，較佳地該劑量是約0.1微克/千克體重-約100微克/千克體重。當(dāng)然，具體劑量還應(yīng)考慮給藥途徑、病人健康狀況等因素，這些都是熟練醫(yī)師技能范圍之內(nèi)的。
人GPD2蛋白的多聚核苷酸也可用于多種治療目的?；蛑委熂夹g(shù)可用于治療由于GPD2蛋白的無表達(dá)或異常/無活性的GPD2蛋白的表達(dá)所致的細(xì)胞增殖、發(fā)育或代謝異常。構(gòu)建攜帶GPD2基因的重組病毒載體的方法可見于已有文獻(xiàn)(Sambrook，et al.)。另外重組人GPD2基因可包裝到脂質(zhì)體中，然后再轉(zhuǎn)移至細(xì)胞內(nèi)。
多聚核苷酸導(dǎo)入組織或細(xì)胞內(nèi)的方法包括將多聚核苷酸直接注入到體內(nèi)組織中；或在體外通過載體(如病毒、噬菌體或質(zhì)粒等)先將多聚核苷酸導(dǎo)入細(xì)胞中，再將細(xì)胞移植到體內(nèi)等。
本發(fā)明還涉及定量和定位檢測人GPD2蛋白水平的診斷試驗(yàn)方法。這些試驗(yàn)是本領(lǐng)域所熟知的，且包括FISH測定和放射免疫測定。
一種檢測檢測樣品中是否存在GPD2蛋白的方法是利用GPD2蛋白的特異性抗體進(jìn)行檢測，它包括將樣品與GPD2蛋白特異性抗體接觸；觀察是否形成抗體復(fù)合物，形成了抗體復(fù)合物就表示樣品中存在GPD2蛋白。
GPD2蛋白的多聚核苷酸可用于GPD2蛋白相關(guān)疾病的診斷和治療。在診斷方面，GPD2蛋白的多聚核苷酸可用于檢測GPD2蛋白的表達(dá)與否或在疾病狀態(tài)下GPD2蛋白的異常表達(dá)。如GPD2 DNA序列可用于對活檢標(biāo)本的雜交以判斷GPD2蛋白的表達(dá)異常。雜交技術(shù)包括Southern印跡法，Northern印跡法、原位雜交等。這些技術(shù)方法都是公開的成熟技術(shù)，相關(guān)的試劑盒都可從商業(yè)途徑得到。本發(fā)明的多核苷酸的一部分或全部可作為探針固定在微陣列(microarray)或DNA芯片(又稱為“基因芯片”)上，用于分析組織中基因的差異表達(dá)分析和基因診斷。用GPD2蛋白特異的引物進(jìn)行RNA-聚合酶鏈反應(yīng)(RT-PCR)體外擴(kuò)增也可檢測GPD2蛋白的轉(zhuǎn)錄產(chǎn)物。
檢測GPD2基因的突變也可用于診斷高血壓。檢測可以針對cDNA，也可針對基因組DNA。GPD2蛋白突變的形式包括與正常野生型GPD2 DNA序列相比的點(diǎn)突變、易位、缺失、重組和其它任何異常等。一類優(yōu)選的突變是表2所示的SNP。可用已有的技術(shù)如Southern印跡法、DNA序列分析、PCR和原位雜交檢測突變。另外，突變有可能影響蛋白的表達(dá)，因此用Northern印跡法、Western印跡法可間接判斷基因有無突變。
本發(fā)明的主要優(yōu)點(diǎn)在于首次用實(shí)驗(yàn)證實(shí)了GPD2的改變與高血壓的密切相關(guān)性，進(jìn)而提供了輔助性診斷高血壓(或易感性)的方法和試劑盒。本發(fā)明可以為臨床診斷(尤其是早期診斷)高血壓提供極為有價(jià)值的輔助性參考指標(biāo)，從而有利于實(shí)現(xiàn)早診斷、早預(yù)防、早得益。
下面結(jié)合具體實(shí)施例，進(jìn)一步闡述本發(fā)明。應(yīng)理解，這些實(shí)施例僅用于說明本發(fā)明而不用于限制本發(fā)明的范圍。下列實(shí)施例中未注明具體條件的實(shí)驗(yàn)方法，通常按照常規(guī)條件如Sambrook等人，分子克隆實(shí)驗(yàn)室手冊(New YorkCold Spring Harbor Laboratory Press，1989)中所述的條件，或按照制造廠商所建議的條件。
實(shí)施例11對象與方法1.1研究對象1.1.1中國人SNP發(fā)現(xiàn)和確認(rèn)樣本樣本為來自24個(gè)核心漢族高血壓家系、無親緣關(guān)系的24例原發(fā)性高血壓病人。
1.1.2SNP分型和關(guān)聯(lián)分析研究樣本所有受試者均為漢族。原發(fā)性高血壓組(EH組)和正常血壓組(NT組)各300例，均來自中國上海地區(qū)，彼此無親緣關(guān)系。EH組為上海市瑞金醫(yī)院高血壓科住院病人，均為漢族，無親緣關(guān)系。平均年齡為57.32±11.75歲。入選標(biāo)準(zhǔn)收縮壓140mmHg和/或舒張壓90mmHg，或正在接受抗高血壓藥物治療至少一年以上；在30歲之前或60歲以后發(fā)病者除外；臨床上除外繼發(fā)性高血壓，如原發(fā)性醛固酮增多癥、嗜鉻細(xì)胞瘤、腎性高血壓等。
NT組為上海地區(qū)40歲以上居民，均為漢族，無親緣關(guān)系。平均年齡56.39±6.45歲。收縮壓＜140mmHg且舒張壓＜90mmHg，無服用抗高血壓藥物史，排除肝、腎功能障礙、甲狀腺疾病、糖尿病、冠心病史。
1.2實(shí)驗(yàn)方法1.2.1DNA提取常規(guī)酚-氯仿法提取外周血白細(xì)胞DNA，并標(biāo)化DNA濃度值至20ng/μl。
1.2.2PCR及測序引物的設(shè)計(jì)根據(jù)GenBank中GPD2的基因組序列，應(yīng)用prime3軟件(美國麻省理工生物醫(yī)學(xué)白頭研究所，http://www-gonome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi)設(shè)計(jì)引物。具體引物如下表所示。
在25℃下干燥24小時(shí)之后，通過蒸發(fā)乳液而制得牢固粘著在玻璃或鋁材上的具有粘著性能的類似膠體的彈性薄膜。
實(shí)施例2至6利用下表內(nèi)給出的量，以類似于實(shí)施例1的方法制造其他乳液表1

*nb代表未測得
1.2.4SNP的發(fā)現(xiàn)和檢測PCR產(chǎn)物經(jīng)Resin樹脂純化后，用ABI-PRISMTM377 DNA測序儀(美國應(yīng)用生物系統(tǒng)公司appliedbiosystems(ABI)進(jìn)行熒光標(biāo)記末端終止法雙向測序，用Polyphred軟件(美國華盛頓大學(xué)http://droog.mbt.washington.edu/Polyphred.html)進(jìn)行序列的判讀和SNP確認(rèn)。
1.2.5SNP基因分型和關(guān)聯(lián)分析用直接單向測序法進(jìn)行SNP基因分型，即在300個(gè)高血壓病人和300個(gè)正常血壓對照組中進(jìn)行分型和關(guān)聯(lián)分析。
1.3統(tǒng)計(jì)學(xué)方法SNPs各基因型在病人和正常血壓對照中分布的比較采用R×C表χ2檢驗(yàn)。P＜0.05認(rèn)為有統(tǒng)計(jì)學(xué)差別。
2.結(jié)果2.1GPD2基因SNP的發(fā)現(xiàn)和分布GPD2的基因共有17個(gè)外顯子，測序總長度是8586bp，共發(fā)現(xiàn)8個(gè)SNP，包括啟動(dòng)子區(qū)3個(gè)，編碼區(qū)2個(gè)，內(nèi)含子2個(gè)，3’UTR1個(gè)。其中編碼區(qū)、內(nèi)含子區(qū)和3’UTR的7個(gè)SNP(SNP02，SNP03，SNP04，SNP05，SNP06，SNP07和SNP08)都是新發(fā)現(xiàn)的SNP。GPD2基因SNP分布情況見表2。測序確認(rèn)雜合子圖像見圖1。
表2 中國人GPD2基因SNP的種類及其分布

說明(1)表中第一列為SNP的名稱；第二列為SNP在GPD2基因序列中的位置；第三列為SNP所屬基因區(qū)域，exon為外顯子區(qū)，intron為內(nèi)含子區(qū)；第四列為在等位基因中SNP的兩個(gè)堿基；第五列為測序所用引物。
(2)SNP02，SNP03，SNP04(圖1)，SNP05，SNP06，SNP07，SNP08為新發(fā)現(xiàn)的SNP。
2.2SNP位點(diǎn)的基因分型在300例EH和300例NT中，對SNP04進(jìn)行基因分型和關(guān)聯(lián)分析。
結(jié)果提示，SNP04等位基因分布在兩組中有顯著差異(P＜0.05)(表3)表明該SNP與高血壓顯著相關(guān)。
表3 SNP05基因分型和關(guān)聯(lián)分析結(jié)果

EH，原發(fā)性高血壓NT，正常血壓對照3.討論高血壓是遺傳因素與多種環(huán)境因素相互作用而導(dǎo)致的多基因疾病，其遺傳機(jī)制的研究已經(jīng)成為目前的研究熱點(diǎn)。迄今研究者已經(jīng)成功地確定了幾種單基因遺傳性高血壓的候選基因，但占高血壓約95％的原發(fā)性高血壓的遺傳方式和易感基因仍未確認(rèn)。定位克隆法是研究EH遺傳機(jī)制的主要研究之一，上海市高血壓研究所已完成對上海地區(qū)漢族高血壓家系的全基因掃描，研究提示2q14-q23區(qū)域與高血壓存在緊密連鎖關(guān)系，GPD2即為該定位區(qū)域內(nèi)的眾多候選基因之一。
由于多基因疾病表現(xiàn)型的復(fù)雜性、相關(guān)基因的微效性和人群的遺傳異質(zhì)性，其相關(guān)基因定位克隆研究一直是一個(gè)很大的挑戰(zhàn)。在原發(fā)性高血壓候選基因中篩選多態(tài)性標(biāo)記，然后在病例-對照中進(jìn)行相關(guān)分析是目前尋找疾病相關(guān)基因的重要方法之一。SNP數(shù)量多、分布廣、密度高，是進(jìn)行遺傳連鎖分析和關(guān)聯(lián)研究最理想的遺傳標(biāo)記。人的GPD2基因位于2號染色體上(2q24)，編碼線粒體的甘油三磷酸脫氫酶，有17個(gè)外顯子，表達(dá)于腎臟、心臟等多種器官(Ferrer J，Aoki M，Behn P，et al.Mitochondrial glycerol-3-phosphatedehydrogenase.Cloning of an alternatively spliced human islet-cell cDNA，tissuedistribution，physical mapping，and identification of a polymorphic genetic marker.Diabetes 1996；45(2)262-6)。本研究首次對中國漢族人群進(jìn)行了GPD2基因的SNP檢測，發(fā)現(xiàn)了位于啟動(dòng)子區(qū)的3個(gè)SNP、編碼區(qū)的2個(gè)SNP、內(nèi)含子區(qū)的2個(gè)SNP和5’UTR區(qū)的1個(gè)SNP。除啟動(dòng)子區(qū)外的其余7個(gè)SNP均尚未被收錄于NCBI的SNP數(shù)據(jù)庫(http://www.ncbi.nlm.nih.gov/SNP)中。本研究發(fā)現(xiàn)編碼區(qū)的SNP04是一個(gè)非同義的SNP，通過A/G堿基的改變，導(dǎo)致了編碼的氨基酸由精氨酸(Arg)變?yōu)榻M氨酸(His)，提示這一SNP可能改變基因的功能。在上海地區(qū)漢族中，通過病例-對照分析，進(jìn)行它與高血壓間的相關(guān)性研究。結(jié)果表明該SNP的G等位基因在高血壓病例中的頻率為17.5％，在正常人中為13.2％，在高血壓病例中的頻率顯著高于正常人(P＜0.05)，提示GPD2基因該SNP與原發(fā)性高血壓發(fā)生相關(guān)。
實(shí)施例2原發(fā)性高血壓易感性輔助檢測試劑盒制備一試劑盒，它含有PCR反應(yīng)液 含Taq酶dNTP鎂離子PCR反應(yīng)緩沖液名稱 序列(5’→3’)編號 濃度正向引物 gtagatgcctggctgagaatg SEQ ID NO22干粉20D反向引物 gcgtggcataaatgaaagaga SEQ ID NO23干粉20DPCR反應(yīng)液 含Taq酶dNTP鎂離子PCR反應(yīng)緩沖液抽取待檢測男性病人的血液3ml，使用常規(guī)方法(或使用特定的試劑盒)從血液中提取DNA。將高血壓檢測試劑盒中的PCR引物稀釋到1μmol/μl，以所提取的DNA為模板與所提供的引物進(jìn)行PCR反應(yīng)。PCR產(chǎn)物純化后，用ABI-PRISMTM377 DNA測序儀進(jìn)行熒光標(biāo)記末端終止法雙向測序，用Polyphred軟件進(jìn)行序列的判讀和SNP確認(rèn)。檢測結(jié)果圖1所示。其中，含C表示高血壓易感性高于正常人群。
用本實(shí)施例的試劑盒對近百例待測對象進(jìn)行了SNP04的檢測，對于檢測發(fā)現(xiàn)存在115330位A→G的3個(gè)對象，再對其進(jìn)行血壓測量，結(jié)果證實(shí)2位具有115330位A→G的人的血壓高于正常人。
在本發(fā)明提及的所有文獻(xiàn)都在本申請中引用作為參考，就如同每一篇文獻(xiàn)被單獨(dú)引用作為參考那樣。此外應(yīng)理解，在閱讀了本發(fā)明的上述講授內(nèi)容之后，本領(lǐng)域技術(shù)人員可以對本發(fā)明作各種改動(dòng)或修改，這些等價(jià)形式同樣落于本申請所附權(quán)利要求書所限定的范圍。
序列表<110>上海市高血壓研究所國家人類基因組南方研究中心<120>甘油三磷酸脫氫酶基因與原發(fā)性高血壓的相關(guān)性<130>052514<160>1<170>PatentIn version 3.1<210>1<211>149000<212>DNA<213>智人(Homo sapiens)<400>1aaggaagttt ttaaaggaaa agaagaggca gtttctaaat tgtttaccaa taatttacat60aaaaaaacaa actattaatt gtctatagat tgttcttcgt atcacaaatt ccaggaacat 120gaagatatgg ttgggtagtc agtcatgaac aaaatgcctt taaacaatcg cctctgagca 180tgagtgtggg gggtgcaact tatgtcccat attcaaggtc tctctgggcc tgataaattt 240tgcatacctc acattgctca aatgctctga actatttttc ttttctcaac tacaatttcc 300tttttctggt tgctcatgat tcaactgggg gagcttttaa accgtggttc tcaaacttta 360atgtgcatca gaactaccca aagggcttgt taaatctggg tagctcagcc ccagccccag 420agtctatgaa tcaggtcact ggagtacagt ttctgtcaag ttctcaggtg atgctgatgc 480tgcgggctcc agtgactaca cttttagagc cagttttaaa atacaaggac ttgcagatcc 540cacgtccaga ctgactcatt ggacttgggg atggaaatct ggcattccac ccctccctgc 600cccctccccc caattttaat gtgtccaagg gtgagaatca ttgctcctct gtggactatg 660gatggttccc tgaagttact gagtttggga tttaataaat atatgcattc ctctgggtca 720actacaggaa ttctgcggta tttaaggaca ttaaatgagt gacttaatac tttatttaaa 780aaaataccac tgctttttgg ttcacactaa ttttcttcct tcttttggaa aggcatttcc 840gtccatctcc tggatatttt ccaataccac gatagaaagg ttattagtac agcacccaga 900ggtcttcgga gcaaagctgt taaagaagtg gggtccattt gagccccaac tttgccttcc 960ttaggtcccc ttgggcttgt ctagccctag gagttccaac aagtaagctg ggcatgctca 1020gtgggcaggg tcgttttttt tttttgggtg gcggggggtt gcaagtggga agcctgctgt 1080tcagctgccg gggctctccg cctcccccca cctgtatgag gctgggtctg gggaacctgt 1140gctcagcatt ccaccccctg gagcttgggc ttggtcttcc ctgcgggtcc ctgcgctgac 1200attcaggcgg ggagccagga ggcctggcgc gcctccagag cccgccgggg gagccgggcg 1260agggttctgg gctctgacgg cggggtcgca gggtcgcccg cctcctggac acgtctgtag 1320gcctagggaa gcctgccggc cgggaggtac agagtaggag aagccagatc ccagggcgga 1380caacgagaag tcgtcaggta agccagcccc gccagcgtgc cggcggacga gtgtctcctc 1440tcccgcaggc agatggccag ggcggcccgc atgggcgagg gcacaggaaa tcggacaccg 1500ccaccgtctt ccccagcctg gcctagctca ggctttccag tccgccagcc cgctggcaag 1560gcggggcgga ggtctggagt ccggcatcgc cgaggcacaa agcaggaact gcagccaggg 1620gcgagggccc ttgcgtgtgg cgctgcgcga ccccacccgg cggcaggacc cgcgtccccg 1680tctagtcacg ttcattgggc cacggagacc acctcgctcg ccgcccgctg ttggcttttc 1740cctcccacac ccccgccaac cccggccccg gttcccgccc ccacttccgc gtttccccgc 1800tgggccggag gagcctgggc ccgaggtatc cgggcaggag gtggcggcct cgccgccctg 1860gtcaggcctc cgggagggag caggcgcggg agcgggagga ggaagtcggg aagagggaag 1920gcgggggcgc ggcccgcccg gcgctcgagt tacacccgcc cagcccgcct cgagtgacag 1980ccccgcgcgc ctcgctggga gcacccgggc cgaggctctg attctggggg gaggccgact 2040ccaccctggc tggaggaact gggtgctcct gcccgctggc ccctcgcgcg tgaggatcta 2100tctcaggtgt gtgcttcctg ggagacccct tcccccgcct ctagtattct agggggggag 2160tggtcccttt ctgacccggg cgagtgttct gggactccag tcttggcggt aggtggtggc 2220gggaaacggg aaagatttag aatttcttcc caccagcaga agatggattc gctcttctct 2280agggtttcgt tgtttatgtt ttggaggcgg catttgtaaa atagtaaagt ggcaggactt 2340ttggggcgag ggttggtacc ccattgaaag ttcatgcaaa cttttagctt gtagatgcgg 2400ctagtctgat aaggattgtt gctcccttcc gaagcaagag tgagaacgag tctggaggag 2460ggctgaggct tctttgagaa actcagctgc tggcgtgtga ccgtcctagg aggaaatgga 2520aggtgcacac tgataactta aaacagcctc cactccctat gtagtaactt ctaccagcaa 2580aaatagattt gcaaagagtg ctttggcagg aaggtgtagc tgggtgaatg aagtgctgct 2640cttagcagat ggtctctagt ctggcttcca ccttgtgcag tggaatattc actcccagaa 2700gagttccaga gtaatttaca aacacaaatt cttgcaaagt gttagtgaca tgggcaaccc 2760aaatcacaca caacacatta taaatatcat aggaggcagc tatagaaata gcagctagtc 2820aacttatact tgtataaata ggtgagggaa ggaagttagt aagtgacagg gagtctttat 2880tgctaaaaca aaactgttag tattttgcat gtgttccaag tttaaagaga tgtgaagttt 2940ctttttggcg tgtggacggt gctggcagct gtcacactgg gcccaggcac ttcttcgtag 3000ctactgacca ctctagtccc agtcctacag ttcttgctgg ctaccacatg acatctggat 3060gccattgccc aatggagacc gtgtgcagtc tggggaacct tgtgatgtgg atgttgaaac 3120ccccctctgt ttctcacagg ggcgttatgt caaattacaa cactcccttg acttttacaa 3180atcaactcgt gaaataatac agcaatattt taatgaataa atgggtaaat atggggtttt 3240gggtttgaga accaacaatg gattcattgc aactgtccat tctcatagct tatagagtca 3300ctttatttta taaagaaatt ctaaacagca ttagagctct gcttccttct ttctcccttg 3360tgattcattc aaagatgcaa tggcttggtt caggaattga caaaagggcc acagtgttac 3420
caattgttag tgagtgttac cacccccttt agtttttttt gtaactattt aatctcattg3480tagatatttt aaaagatttg atgattattg tgcataactc atgttggcaa actattataa3540ctctgaactt atcctgtttg gttgttttct ttcatctcta gcgtgtaagt tctatgagag3600tagcagggct tgttctgtct tgcatacttt caaaactcta atgcctggag tggtgactag3660cttgtaatac accctcaata aatgttttct caatgagtga cctccaatgc ctagaggggt3720gactagcttg taatacaccc tcaataaatg ttttctcaat gagcgactga actgtggaac3780aaactccagc tgagggtagt tgtggagcgg ggagccaaag actttggcct taggtcatct3840tttaaaactc agcctcaatt gtctcattgg caaaatatgg ataataaatg ctatgacgtg3900tgtaaaattg ctagcaatgt acctggcaag tagtagactt caaaagacta gcttccatcc3960tatatttttc cttcttatac tcaagtagga aaatatgggc aaagaatgta gttagcttga4020aaatttgggc aaagaattct tgcatgttgt gcatgaatta tccgtacatt tttttcggtt4080agttgagtgt gttgggatgg ggagaggaga ggcgagttag tggtagctat ggttattaga4140gaagggaagc aattttcaga tcagtatcta atttctcagt tttagttttt gaatgtgatg4200atgtttctgt ttaggttttc caaaacttga ttctttctgt ttgtcaacac ctggactcat4260ggggctatcc taagtgcaga aacaacactt cagaaagagg actagaaatc aaatatatga4320acactagtct tcttaaatga aattttctgt attcttgtgc agcattatat tgtgtaataa4380gtgctaaata aaaaaatagt taacctcttt tgagaagctt acagttgttt aacaaggtaa4440aacatagatg gaaaacaatt ggagaccaac ataagacctc gtacaatcat ttttccagtg4500gtaggatgtt ggccagaact gatgtgatta tgaaggaaaa ggttaatatt ggctcaagta4560gttggagaca gcttcctggt gaagggaatg ccacttcagt aacagagaga cttaacatgt4620cctgaagact attggaaaga tcacctttgt tttaggaatg tttgctattg tgtacttcat4680tgtatgaaaa agccaacata atcttgtact ttcaaaaatg atctttctgt tgaaaattat4740atgggaaatc ttcagtaaaa ttctgcatat gcaagaaatc aatacttgtc actatgtctg4800tgaagtttaa agctcaggat tggcgtgaaa atggtaactc ttcattcttg tccaccttta4860acacacttgc ttaaaaatgt gtcagatagg ccaggtgcag tggatcacac ctgtaatccc4920agcactttgc tttgggaggc caaggcaggc agatcgcttg agcccaggag tttgagacca4980gcctggacaa catggaaacc ccatctttac aaaaaaatac aaaaattaac caggcatggt5040ggcacgtgcc tgtagtccca gctacttagg aggccaaaac gtgagaattg cttgagactg5100tctcagaaaa aaaaaaaaaa aaaaaaaaaa aacaaaaaaa aaaaaacaag ccaggcaggc5160acagtgactc acacctgtaa tcccagcact ttgggctggg aggcctaggc aggcggatca5220cctgaggtca ggaattggag accagcctgg ccaacatggc aaaaccctgt ctctactaaa5280aatacaaaaa ttagccgggc atgatcgtgg gcacctgtaa tcccagctac taggaaggct5340gaggcaggag aattgcttga acccaggagg cagaggttgc agtgagtcaa gattgtgcca5400ttgcactcca gcctgggcga cagaaggaga atctgtctca aaaaataaat aaataaataa5460ataaataaag tgtcagatgg cctgccagtt attctattca gttttgacaa tggggtatat5520tttatatatt ttttacctgg agtataagga gaaaattatt agtcatcagg gttgagaccc5580ctcaacccgc taaattcaaa attgttactt gggagataga gaagaactga atggaactaa5640actaagaaga tattattaag taagttttag ttgctcatca aaatacttaa ataattccat5700catgtcttaa gacttttgag ataagaagaa aacttggaag tggtatgtta ggggaagaag5760aaactaataa agtgtgtttt acttttcttt gttttagaat tgttgacatg atgcgtgaaa5820gaaatgtatg aggaagatat gatcaaactc aactccaagt gagaaagaca agggccaatg5880tgactgcaag ccaggaaatc tctaacaatt ttagaataga cttcttcctg tactatatga5940ccagggagtg atctcaggaa catttaagaa aaagaaagga agagatgttg atggtttgaa6000tacctgagga tgtttagata atgattgtgg tcagaatgca tccagaattc taatttgtgt6060cctgatgtga atgtgaccat gtttgtttgt ttgtttgttt ttgttttccc tttggcttgt6120gttctgggat ttgatctctc gttagagact cttactagtt ctttgagttg tgtatttctt6180tctatatcat atcggtgctc ttatttccta tttttcagta gagaatttta tgttctttca6240aaaccaaatt tctgatttat gcagcaaaaa catgatttct gatttattta ggtcaagctt6300cggttcaatt agttcacaaa attagtggga aaaattggtt aatagatgtc tctccttctc6360aagtacaaat gtattgtgac atattgtgat atagtaagaa agatacttgt ccccagttct6420gactaaagaa cttttaagac ccttcgaatc tctggaatga tgagtgtctt tttctacact6480gctgagatga caggtgactc ttggtagaac cctagatagc ttcaggatgg agatgggagc6540tggtgccaga ggaaccaact atgtaattag agggttggaa tttcagcttc actccagacc6600tccggggaga ggagaggggc tggaaatcca gtcagtcacc agtggacaat aatttaatca6660atcatgccca catcatggag ccttcataaa aaccttaaat tttggaaagc ttctgagttg6720gtgaatacat ggaggtgctg gaaggaaggc atgcccagag aggacacccc ttctcccatt6780tcttgccctg tgtacttctt catctggctg ttgatttgtg tcttttttaa taccttttgt6840aataaactgt tatatgtaag taggcctgtt gtggtggctc acacctgtaa gcccagtact6900ttgggaggcc aagacaggag gataacttga gctcaggtgt ttgagatcag cctgggccac6960gtggagagac cctgtctcta caaaaaatta aaaaagaaaa aactagccag gtgtggtggt7020ccatgcttgt agtcctcact accctggagg ctgaggtgag aggatctctt ggggccagga7080ggtcaaggct gcagtgagct gcgttagtgc cactacgctc cagcctgagt gacagagcaa7140gaccttgtct caaaaaaaga aaaaagataa aaaagtaagt aaatttctgt tagccactat7200agcaaattat caaatttgag gatggggttg taggaacccc tgatttgtag ctaagtctaa7260cagaagtgtg gatatcccgg ggcccactat ttttgactgg catctgaagt ggagggcagt7320ctcatgggac tgagccctta ccctgtggcg tctgcacaaa ctctgggcag ttagtgtcag7380attgagttaa atggtaaaac attctagttg gtgcccctag acagagagtt tcttggtgtg7440gaaaacccat acatttggtg tcagaagtat tgtgactaga ggaataagtt ttctttacat7500atgtattgac aaaacttaaa gacattattt gggtacatct tttaattatt caggcaaaat7560gaaagaatta cttatgtact gtatttttga cagtgagata ctgtcaaagt ctgggtactt7620tagaacaatg attgtttgat ttagattttt caagccttat gttttttgaa tactagtatg7680tcctctgtgg aatctcatat ttgattgtgc acatatatcc ttctataaaa gggtttaata7740tagtgggaaa aacaaacctg acaagctatg acagagagac tgttttctga aatcgagatt7800tctagaagct gctactgtca aatgagataa cctctgagta ttacttggtg acaatggggt7860gacatgctta gaatgctttg ctacttgtat ctgctgatga ccaccaaggc caagtagaaa7920ctttagatag ctatatactt atgtggaaca aaagtttatg ttcagggtaa acttattagg7980aaaaactttg gtcaagattt caagaaaaca taaaataagc ttctaagtgt acttcaaaag8040tgatgaaaat aatgcagttc agagtactag gttctcaaaa gttgttttct tttcatttta8100actgaataaa agaaaggata ttggctaagt gttggctcat gcctgtaatc ccagcacttt8160gggaggccaa ggtaggagga tcacttgagc ccaggagttt gagaccagcc tgggcaacat8220gtgagaccct gtctctacaa aaaataaaaa ataagctgcg tctggtggtg cgtgtctcct8280acttaggagg ctgaggtggg aggatccttt gagcctagga ggccgaggct gcagtgagcc8340atgattgcat tactgcactc cagcttgggc aacagagcaa gaccctgtct caaaaaaata8400aaaaagtaat aaaaaataat aaaaagaaag gatgttttgt ttcattgtgg taatgcccaa8460actgctttga tcatgtcact ctctctgctc catgcttcca tggattactc tgccctattt8520gatcacgttc taaatatttg tcttggaatt aattcagaga ctccttcatt ctcaccctaa8580tattaaggtg ctgtccttat cttctctatg atccagtcat attggactga ttggaattcc8640ctaaatataa aagtaggaat tccctactgt tgtccctatt ctgatgttct tccttctatt8700tcactttttg aaatcctaac aaaccttgag tgccctgacc cctggctact agttttatga8760cattttccct cacagcccca gtcagaaatg tcttgtatat tttctaaacc ctttagctac8820
tctttttatg ctactcataa ttcccttata gatgcttgtc tcctgtaatt atttgcaagc 8880atgcataatt ttctttccta tggaagatca tgctggatct tacacaattt tgtagacctc 8940acatgcctaa tccaagtgcc tttcatgtgg gtgacaccta gtaagcagtt gttcattaat 9000ggttgcccca taattcagtc atccccaatt cagcaataag tgataagagt gtgctgggtg 9060tgattggaca ccagatacca cacaggcagg tgtatttcag gtaatctatt caactggata 9120ctttaagctt catctttttt taggcatctc cctccctaca catttggcag ttttaatctt 9180tttattttta tttcaaaatt tttttccctc ccaaggtgct gagaggttta atatcttaag 9240gatatttagg agagggaagg ggcgaagtga cattataaaa ggaatccttg aagaaaatca 9300gtggaggaat ctacaaataa ggaggccact taatgtccac tgaggactca gcacctctgt 9360caggacacca gggcttcctt ctcttcacca atttctccct tccccctact tacccaccta 9420atccaatctc ttaagaaaga atctggaaaa tgaattccta acaagatgac ttacaaaaat 9480taccttgtcc cccttatcaa aggcgttgga ttctatgggg acagcgcatt ggggaccatt 9540tatgtgggct aggtcaaagg tgggcagtgc ctacttctga gcatccctgc agccacatta 9600tagccactca agtgtggctt tggtcactgt ctggaccttt cacttttctc cttgtcaaat 9660aaggtggctg gactggatga atttgaagtc catggagagc atgaaatgtt tatttctctt 9720gtcctgccta aaaggaagcc agtcatggcc actgggttct gtttgcctct ggaccctggc 9780ctagattttc cttttattac ctggagtttg gggtttcctt gctctcagcc tctccaggta 9840tagcattcac atctagaggc tgccacattc cttttatctc tcttcaggat tatgctcata 9900ggacctatca taacaagtat attttcactt ttttctttca ctgtgtttgc tagtattaac 9960cccttttccc catccccatc cgccattaag tagctgggat acttatctaa aaaatataga10020ttccgcaggg ggcagtggct catgcctgta atcccagcac tttgggaggc cgaggcaggt10080ggattgcttg aggccaggag tttgagacca gcctggccaa cgtggcaaaa ccctgtctct10140actaaaaata ccaaaataag ctaggcgtgg tggtgggtgc ctgtattcct agctactcgg10200gaggctgagg catgagaatt gcttgaagct gggaggtgga ggttgcagtg agcggagacc10260acgccattgc actccagcct gggcaacgga acaagactgt ctcaaaaaca aatacacaca10320cacacacaca cactaacata attttattta ttattatttt taaagacaga gtcttgctct10380gctgcccagg caggagtgca gtggcacatt tatagctcac tgcagctttg cactcctacg10440tcacgcgatt cttctcctca acctcctgag tagctagaat gacaggtgtg tgccaccatg10500cctggctaat ttaaaaaaaa ttcttagaga cagtgtctca ctatgttgcc caggctggtc10560ttgaactcct ggcctcaagt gatcctcctg cctttgcctc agaagtgctg ggattacagg10620catgagcccc tgtgcctggc cagattcaca gaattttaaa ataggaagga gtttagcaat10680ttaacctaac gtggtctttt tcttattaat aataaacagc aatgacaagt atttattaag10740cagttgccat tttctaggca ctgtgctgag tactttgtgc acatttttta atgtatcaac10800agtgaaccct gtcatatcag ggagcaaaa ggcacagaga agttaaggga cttaaagcca10860tgcatgtggt tattttctgg tatggcagaa actcccttaa aaactgtttt aaaaacaata10920gaatcgagag ataatcctat tcctatttct gtaatgtatt tcttgacttt tgcaacattg10980tattccactg cccaaccatt gaactgtgcc atttaacact tacacttcct aatactttac11040cttgtagttc accagttttc accaatgatg ctgaacttgt ttaaatagtt gcatacatag11100cacagcatgc ctataatgac attgcttatg atttgacttg taggaatcca cgaagttgtt11160aaaattttct tagtcttaat ggatgtagaa gcaatgtgca acttggtatc agtcttcttt11220tctactccta tacttgggtt tctgatggtt attgtgaaaa cgaaaatgat tatgtgtatt11280ggagatgtat aaaaatgtat gttctccagc cttagtcatg agagagttat cttacctttt11340tctacacaat acctttttct atttcttgct gtagtttttt tcaaactttc agcattttct11400ttgagtcttt tatgtcaccc aggcatttct ttattaaaag atgatggtta gtggttaggg11460taggctcaac catcataact gaaaggcctg aaaatagata caagctcaag cacaagttta11520atttttgctt atatcatggt ctttgttggt ggatcacttt cctttcaatg attcaggcac11580tctggattct tccattttat gactcagcaa ttccttaggg ctttcttttc aactacagcc11640agccagtgga tggtgaaagg gaaaggcagg aagcatatct actttctaaa cacacactta11700ttttttttga gatgagtctc gctctgttgc ccaggttgag tgcagtggga cgatctcagc11760tcactgcaac ctctgcctcc tgggcttgag cgattctcct gcctcagcct cccaagtagc11820tgggattata ggcatgtgcc accatgcccg gctaatattt gtatttttag tagagatggg11880gttttgccat gttgaccagg ctggtctcga actcctgacc tcaggcagtc cgcccgcctc11940aacctctcca agtgctggga ttacaggtgt gagccactgc gcctggcctt aaacacataa12000tcttgaaatg aatgcattag ttctgttcat ataccattgg ctggaagtct gttgtgtagt12060gatacttagc tgaaagggag attggcacat gtagtctagc tgcaagtcca ggaagaagag12120aatatgcatt tttgataatg acttagctgt ccccttcaca gtgtccttgc attttttttt12180tttttaagat ggagtctggc tctggcaccc aggctggaat gcagtggcac gatcttggct12240cactgcaacc tctgcctcct gggtttaagc ctcagcctcc agagtagctg ggattacagg12300tacgtgccac catgcctggc taatttttat atttttagta gagacaaggt ttcaccatgt12360tggccaggct ggtctcgaac ttgtgatttg cctgccttgg cctcccaaag tgctgggatc12420acagacatga gccacagcac ccggcccttg ccttctatta ttctgagaga attgaaataa12480catgtcaaat cttaaaattt ttctcctttc cacatcattt ttatatctga atccatcaac12540ccctcattaa ttcttgactt agaggtggtt atccgaagtt gatgctttcc cccgtactct12600ggatccccca ttcccttcag atgccttatt ctatgacatt tttctatgct tacctgcatg12660tatttatttg tttgtttgtg ttggagatgc tgagatgcat ttcaaatatt tacaagaatg12720ctcaagattc tcccattaca aaaacaaaca agaccaataa ccaacattgc acctgtgccc12780cattctgccc tcttgctaca tgctctcttt ctcctttcct tcaagctgtt cttccttaaa12840gtactttttg tgcattattt ttctacttct tcaccttcca ttccctccac aactgttgga12900atctgtcttc gatctcagat gaatctctct cttttttttt ttcctgagac agggtcttat12960cctgtcactc aggctggatt gcagtagcac aatcatagtt catggcagcc ttgaactcct13020gggctcaagc gatcctccta cgtcagcctc ctgagtggct gggaccccag gttcaggcca13080tcatacctga ttgattttta ctttttttat agagatggag ccttgccatg tggcccaggc13140tggtcttgaa ctcctgggct tgagcgattc tccctcctta gcctcccaaa gtgctgggat13200tacaggtgtg agcctggccc aactctgctc ttgttatgat tatgagtgtc ctcttgactg13260acaaatcaat gagattttat tatttaaaat ttccatgatt ctgctcattc tcttttctgt13320gaaactgagt tttcactagg ctactgtgac atcttctctt ggattactcc ttatggatct13380ctctgcaggt tctttgcctt gatattggaa ttcccagggt ctgtctttag ttcactctct13440tttctctgtt gatgcttttt caacaagttc atcaatatcc ctgatttcag tcaatcccaa13500gtcaacattc ttgggcagac ttattcgctg aactcaggca tttccacttg catgtcccac13560agttgagtca ggacccataa tttcttcctg ctttcccatg ctattccttt ccttattgac13620aaatgccatc atcttttctc tcactgccta catcatccat ccatttgttt gttaggtcta13680tcaatcacct aatatgtgtc agcaattttc cctttttttt tttttgactc ttgctctgtt13740gcccaggctg gagtgcagtg gcatgatctc ggctcattgc aacctccgcc tcccaggtac13800aagcaattct ctgcctcagc ctcccgagta gctgggatta caagcacctg ccaccatgcc13860cggctaattg ttttgtattt ttagtagaga cggggtttca ccatcttggt caggctggtc13920ttgagctcct gacctcgtga tccaccacct tggcctccca aagtgctggg atgggattac13980aggcgtgagc cactgcgcct ggcctatgtc agcaattttt ctacaaataa gattatagtg14040gtgtacaaaa caaacacagt ttctgttctt gtagaattat gttttagtgg ggaacaaaga14100aataataatg cttaaataaa ggagagacat gagaagataa gagttgtatg catactttgg14160atttgaataa tttgggaagg cctctctgag gacttgacat ttaagctgag tcgtgaatga14220
ctggaaggag cttgccatgc aaagttgagg agcagagcat tccaggtaga ggcagctggc14280cccaaaacac taatagtaat cctgatttga cagacatcag aaggcaaata aatctggaac14340ttagtgggta agagtgagag gtgtctgtat tggtgtcctg tggctgctgt aacaaatgac14400cacaaacttg gtggcttaaa acaacagaaa tttattcttt catcattcag gatgccggaa14460atctgaagtc agtttcactg tgccaaaatt aaggagttag caggatgtgc tcccttctga14520ggagtgaata tggtccttaa ctccttcagg ttctggtggc ttctggcatt ccttggctgt14580ggctgcatca ctccagtctc tacttctgtg gtcacatcat tgcattctca tctgtatcaa14640atcaccctgt cttcttctta caaagatgta aagtgattac atttagggtt ccccctggta14700attcagaata acctttccct cttaaggtct taatttaatc acattggtga attttttttt14760ttccttataa cattcacagg ttccagggat taggatgagg atatgttgtg ggcgtcatta14820tttaacctac tgcagtgaag ggctggatcc tataggtctt tataatccaa ggtgagatgt14880ttagatttca ttctagggtg ctagaaacca ttagaggatt atatgcaggc aagagaaact14940gtgtggtttt gctataaagt agtctttctt aatttcttct cactgtacct ttccaagaag15000aaaaattaaa ttaaaattaa ttaattaaaa acttaatttc tccccaacaa gagagtactg15060cttaaatgct aagaagattt tgttgggtag agttgaagtt tggagggcca cagacaattg15120taatatgcaa tattttttca cctgccaaga agcaactgtg ggtgatattg ctctcattga15180gaatacatgc catcaaagat tatgctggct gctacaagta aacagaatat gggaagtggg15240agttagttag tctgttttgg ttgcccaacc aagaacttat tattgaatgg acaaccattg15300tattaaagat agaaaaaagt ggcctgggcg tggtggctta tgcctgtaat cccagcactt15360tgggaggctg aggcgggtga atcacatgag gccaggagtt cgagaccagc ctggccaata15420tggcaaaact ccatctctac taaatataca aaaaaaaaaa agccgggcat ggtggcacat15480gcctgtaatc ccagctacta gggaggctga ggcaggagcc ttgaacctgg gaggtggagg15540ttgcagtgag ccaagattgt gccactgcac tccagcctgg gtgacagagt gagactctgt15600ctcaaagaga aaaaagcata gaaagaagtg gatagattca ggatatattt ttgagataga15660attagttggg tgaacttgcc tgctagatta tttggaggaa aggaagaaga ataaaagaaa15720ggcttgcatt ttctatttga gagctggtcc acttactgag aaggggagag ctgggagaga15780ggcagacttg atggaagata taaagtgttc tgctttggct aagtttgaag tgattattag15840atattccttt ggagatgtca gacaactggg tgaatggggt ttggagattc tgagaggggt15900ttggctgagg atacagactt aggaattatt tgcatatagg ttatatttga tactggatgg15960agttacctac agagaatgta tatagagaga agcaggccat tgagcaacta ttatatagaa16020gagaaggagg agttgccagt gggatgggat ggaaagcaga agagcgtggt gtctctgagg16080ctaagaattc aaaagatatt aaaaaagaga cggttctact atgttaatta tagagattac16140atttggtgaa atgaggtcat ttgtgatttt gacaatttga catatagttt aagaagaggc16200tgggacaaag tttattggat taaattgtgg aagtggagac agttcttata gacaactctt16260tttttttttt tttttattgc tcattcttgg gtgtttctcg cagaggggga tttggcaggg16320tcacaggaca atagtggagg gaaggtcagc agataaacaa gtgaacaaag gtctctggtt16380ttcctaggca gaggaccctg cggccttccg cagtgtttgt gtccctgggt acttgagatt16440agggagtggt gatgactctt aacgagcatg ctgccttcaa gcatctgttt aacaaagcac16500atcttgcacc gcccttaatc catttaaccc tgagtggaca cagcacatgt ttcagagagc16560acagggttgg gggtaaggtc acagatcaac aggatcccaa ggcagaagaa tttttcttag16620tacagaacaa aatgaaaagt ctcccatgtc tacttctttc tacacagaca cggcaaccat16680ccgatttctc aatcttttcc ccacctttcc ccccgttcta ttccacaaaa ccgccattgt16740catcatggcc cgttctcaat gagctgctgg gcacacctcc cagacggggt ggtggccggg16800cagaggggct cctcacctcc cagtaggggt ggccgggcag aggcgcccct cacctcccgg16860acggggcagc tggcagggcg gggggctgac ccccccacct cctttccgga cagggcggct16920ggccgggcgg gggtgctcct cacttcccag taggggcggc tgggcagagg cgcccctcac16980ctcccggacg gggcagctgg cagggcgggg ggctgacccc cccacctccc tcccggacgg17040ggcggctggc cgggcggggg gctgaccccc ccacctccct cccggaaggg gcggctggcc17100gagcgggggg gctgaccccc ccacctccct cccagacggg gcggctggcc gggcgggggt17160ctgacccccc cacctccttc ccggacaggg cggctggccc ggcagagggg ctcctcactt17220cccagtaggg gcggctgggc agaggcgccc ctcacctccc ggacggggca gctggcaggg17280cggggggctg acccccccac ctccctcccg gacggggcgg ctggccgggc ggggggctga17340cccccccacc tccctcccag acagggtggc tgccgggcgg agacactcct cacttcccag17400acggggtggc tgccaggcgg aggggctcct cacttctcag atgggcggag ggtctcctca17460cttctcagac ggggcggccg ggcagagacg ctcctcacct cccagacggg gtcgcggccg17520ggcagaggcg ctcctcacat cccagacggg gcggcggggc agaggcgctc cccacatctc17580agacgatggg cggccgggca gagatgcttc tctcttccta gatgggatgg cggccgggca17640gagacgctcc tcactttcca gactgggcag ccaggcagag gggctcctca cgtcccagac17700gatgggtggc caggcagaga cgctcctcac ttcccagact gggtggcggc cgggcagagg17760ctgcactcct ggcactttgg gaggccaagg caggcggctg ggaggtggag gttgtagcga17820gccgagatca cgccacctca ctccagcctg ggcaccattg agcactgagt gaaccagact17880ccgtctgcaa tcccggcacc tctggaggcc gaggctggcg gatcactcgc ggttaggagc17940tggagaccag cccggccaac acagcaaaac cccgtctcca ccaaaaaaat acaaaaacca18000gtcaggcgcg gcggcgcgca cctgcaatcg caggctgagg caggagaatc aggcagggag18060gttgcagtga gccgagatgg cagcagtaca gtccagcttc ggctcggcat cagagggaga18120ccatggaaag agagggagag ggagaccgtg gggagagggg agaggggaga gggagaactc18180tttcaagaag tttcattatg aaggaaagca gagacgtgga aaagttcttg gaagaggata18240ttggtcaagg gagggtttgt tagaactaga ggataataca gtgaatttct atgctgacaa18300aatgattaag tagagaagag gagctgatga aggtaggaga gagtggtggg taattgtaca18360aataaaattt ctgaagagcc aggaaggaag gggatctagt gtacaaatag aatgattggt18420ctttacttga aacaggcaca ttttattagt aaaaggaagg actgcagaga gaatgagtac18480agaggtggat aagtcatcat ttgggtggtg ggaagatgaa atttttacct agttgcatct18540actttacttt cccagtgaag tgtgatgata ttggggtgtg gaggggtgaa acaggtttgg18600gaaaagagat ttcaaatggc tattttgaaa catcctgggg aaatgtaata ggattgtggg18660tcagtgtgga ttgcacatta atgatttgca gctatgaatt taaattgagg ccagttgtgg18720ttgcatgttt tttttcccag gcagtttctt cagctgtttg ggtggagatg tgaaacaggc18780agatagttgg gtttaactgg tttgggggtt tgccaagtaa aaatgaagca tgaagagagg18840agcaagggag ttaagggtgc ttggaaggga gaaattacag tgatggacct aatttaattc18900tcagctaggc agcaggagaa gtgactgata gtgaagcaaa tagtagggtc aatggactga18960agggccagat aatgccaaga tggtggaatg gaaattccag actacagtag ataaactgaa19020gaaagagaag gtggtgttta cagtgtaggg tgcttgaggc tggagtctgc ggagttgata19080taaatagtgg ggatgcccaa atcagaggtt atgataatga ggtagggtca aacaaaggtc19140atcagaggtg aggtggttga ggaattggag ccttcatgta gttgtagaaa acagcaatga19200tggccgggcc tgtaatccca gcacttcggg aggctgaggc gggtggatca cctgaggtca19260ggagtttgag accagcctgg ctaacatggc aaaaccatgg ctctactaaa gatacaaaaa19320ttagctgggc atggtggcac atgcctgtga tcccagctac ttgggaggct gaggcaggag19380aatcgcttga acccgggagg tggaggttgc agtgagctga gatcacgcca cttcactgca19440gcctgggtga cagagtgaga ctctgtctca aaaacaaaaa acaaaacaaa aaagaaaact19500gcaatgatga agacagatga gtctggaaag ggaaataggt atgaaggtca tcagcaaata19560aggggaaata gcagtagttg ggtagatgac agcgagaagg gataatgggt ggtaaagtct19620
aatggcataa acttcagaca ggtaagactt ttgaaagagg atagaagaga aattggaagg19680tgagctggag agacacttaa tccacctcta ggttagggga tcttggggtt atgaacagag19740tcgttactca aagtgtttga caactcatgt gaaatggtaa cctgctgatg tcggtgttcc19800atcaggagca gggttctggg ggccttttgt gaagctagat gttaactctt taatctcttg19860atcatagcta gttgtcaact ggttgagagg tattttgata ctttagcaac tgccatagcc19920atccttgttt atactggctg aaactagacc taagtatgag aatatagggc catgtgtggt19980gtctcaggcc tgtaatctca gtgctttggt aggccaaatg ggaggattac ttgaggccag20040gagttctata ccagccaggg caacatagtg agaccctatc tctatgaaaa aaaaaaagtg20100ataatataac caagcttctc tttatatgat atgggaaaat agtgttatta gtgttcagca20160tctgaaattc aggttaaatc ttactttctt tatgttttca aggtttagtt tggtgaaagg20220aacactcaga gaagagaatt taatggaatt tgctggggat agagtttaag taccagaggg20280cagagtggga gggagggagg cctgggaagg tgagagatgg ggaagattgg tcttctggtg20340atgactgagg aaaagagggc tgtggagcat gctagggtta gtcctggtga tgtcttgggg20400gaaattgggt aggtaccatg tggcagctcc tgttgcttag tggccagggg tgagcaaaag20460ccttcttagt gtgtctgtag cctaggtcat ctcctgaaga cgtagtggcc agttggagta20520taaaggcctc cttggggacc agctgccctt cagtggctga tagcatgagg tgctcttagg20580gcctcttctc agccatgatg gtggtgtaac cctgggggag agggaggggt cttctgtttg20640aaggactaag gactgctgct cccagggagc agtcatctta gcaattctcc ctccaaaatg20700gatttggact tcgtgctgtc ccctctgtaa tgactgtggt tgtcttagta tggactctca20760ggagttgcgt ccagtctcct gactgccctg aatccagtct tctgatttcc ttactcccag20820attcatgttc ttttagttta tctgtcaaga ctgctctgga aaattaggaa tgagagaatg20880gtcttttaag ttcaaccatg gttctatcta atcctactag gaatgttatg aagatgaatg20940taacatatat aaattgacta gcacagtcct tataacacag tatgtagcca aagcatatgc21000ttaaagcctt cttgacaatc ctttgtctaa tgacaaagtc caaattcctt tgactcgtat21060ataagatttc cagtgattct tctgccttgc tctattgagt ttcattgcct ccttacttag21120gattactcat tccaccctat gccctagtca gaccacaccc tcttgttctt gaaaatgatg21180tgttgtctta taccattatg tctctgctca tagtcactct ggacataata caccttcact21240gttaaagtta catattgcct tccctagaaa cctcccctga tgcttcctgc cttctctttc21300cttcaggggt aagtaatccc ttagcgcctt gtccttattt ttatcatttt tgtttgtgtt21360agaatgcttc tctctaagcc attagttttt taaaggcaga aaccagtaag tgattcatct21420ttgtttaccc atggtctaat acagtaggtg cccacaaatg tttgttgagt aaatgaaaac21480tcactgcata tttgagtttc catattgcct ttgtttggtg aaatctaaat tatatatttt21540ttgcttcttt cttttaacag tgcaggaaat ttttctgatg aagtagtaga tgagtgcaga21600aaaaaactga tgtccatgta gttatttagg atgggaaaca gacttcatcc tgtgttcttt21660tccttctact gagtgacttt tactgcttga tttatccagt gattttgctc actattgcca21720ttgttttttg gtgaattgta atcattaacg ttttcagtgg tacaccaaag aaatgcaaac21780aaatgtgatt ttctggttgt ttaattgggc attttctggt gcccacttag aattgtggga21840ttggggtatg gagcattcta gaatgttggt gaaaagtcta gtcctttagg tttcttcaaa21900tgttggtctt cttgggccag gcgcggtggc tcacatctat aaccgtagca ctttgggagg21960ctgaggtggg cagatcacct gaggtcagga gtttgagacc agtctggcca atatgacgaa22020accttgtctc tacaaaaata caaaaactag tcgcatgtgg tggcgcacaa ctgtaatccc22080agcttctctg gaggctgagg tgggagaatt gcttgaactt ggaaggtgga ggttgcagag22140atcacaccac tgcgctccag cctgggcaac ggagtgcgac tctgtctcaa aaaaaaaaaa22200agttggtctt cttgatccta catgaactaa ttaattctgt attccaaaga attaagataa22260tgcaaatcag atggcagcag aatgaatcaa gtaagagtgg gaagggcaaa tgtgaaatga22320gggggcagga gtgagtcact cttttgtgta gatggatagg gaatgctgga aaggggaggg22380tgaaggtggg ttgggggctg tgggagagtc tgagcaataa ggagatggcg tagcatgggt22440aaaggtggtt tgggcgacac agtggttagc aagctctgtg gttagcatgt tctgcatgag22500tgaccaagca aggcccattt tggcacttga gatgggatca ctgaccctcg ttccccagtt22560ctcattgtaa ggttaaacaa agatgcagtg tatgctttca tattttgaga ttagggtatc22620attctagaag ggacacattc ttgagaaata gcttgatttc tttaaaaaaa aattgactca22680tagactgttt ccatggcatt ttgatttttt attagtattt tttcctaagg tacttagatg22740gtgtccagag tttgtgacag agccagaaag caactcccca ccttctctga aataaaaata22800aataccaaga agtactattc taaatagagg accagaaact actcatatgg gtcaaagttg22860tcccaatgac ttgggttaac tcaggccacaaa cctttgct caggcttctt acagggaata22920tttttaatct gccaattgac agactactag agatagaggt ataaacgttg gactccatgt22980gattagagcc tgatctgaag ggaaaatcct cttgcctcct tctgacctct gtaacaggta23040atctgtgacc cagaaataga tctgcattag atgttgggct tgatatgggt ttatttttga23100tagaacactc tgaaataaga gtgtccattc tatttgagtg aatgtttatt tgggggaaag23160atgctagaga tatgccagaa gaggccgcct gcctgtgtct tttcagggaa acaatgggat23220ttatttcccc aacctgtgag tgttaagtgt acttggttgt tcagaacaaa gtgctggtta23280ggccaaagca gggttgtggc ccgatggaaa ttggttggtt gcttttgctc tctcgtaaca23340atagtcttct atatcccaat tccacgtgca aatgtggaca atgtgtgaaa aggccagagc23400ttattgtaga tgctcagtaa tgttatgtat tagcagtggg agttgttgga tagcagatgt23460atttttatgc tttacttata ttacctcgaa ttctcagtat aacttgtgac aaaagagatt23520ttataaatga ggaaactaaa gctcatagtc acacagctag taagtggcta agttggaatt23580cacccagggc tgtctgtgtc ttctgtctcc tacactggct gcctcctgca ctgaattttc23640tcctgggtgt cattggtgtc atttgaatat gccgaggaca gcagcctagg actagcaggt23700ccctggctgt gcctatagga gtagctgtgt tcctgcaggc aagcactaaa ttatcacagg23760gtggggagag agagggaatg ttggccccca ttttaggatg gtctgtctct tctctgtgca23820tctgtggact tcctttaggg cctggcacag tggaggggcc ccctgggtgt gttaactaaa23880gagattctgt agttatggag ttgaaatttg ggaaacaccc aattccagga aggttgatca23940gctttgcagt ttctggtttg atttgttgtt gggatttcca gtgttgcatt aaaactgcaa24000atccccgagg ggctgtaatg tgcaataact aaaaatgtag ggcgagttta ggtgcagctt24060aactcaagct taaatggtta ttgtgtccca tgctcaaggc aaattcattt tagttagggt24120ggagattttt ctagaatgag gcagataatt ctgtgacatt ttccagttct tactcaaaga24180ttcctaataa attgtaaagg caaagtattc tttttctttt tctatatggg tcttttactg24240tagttcatta aagatgagaa aaagttgtat aacaggaaaa tcactcaagt aatggtcaga24300tgatatggga tttggtcaaa tgtcagctat ccatctgcat ggtgctcttt ggaattcttt24360tttttcaggt ctctttctct tatatgcatg cacacgtata ttccacattt ttttctaatt24420acaagaataa ttcatgctta ttttagacat ctagaaaata atgcacacaa ggaagaaaat24480aaaaatgatt cataatacta ccattcaaag atgagatttt taacattttg gggtatattc24540gtctaacctt cacttgtgag tgtgtatgtg tgtgtatgta catatacttt aaaatagaaa24600tatatatata tatatatata cacacacaca aagatactgt tttatttact tttgttctct24660tcataattaa ttttctactt gtccgttacc ccatctctat ccagagtgta cttaactcag24720aaaggattaa ttcctacaag gtgaagaaat tctagaatgt ctaacttatt taagggtgcc24780atttattttt ttagactttt ctttattatg attcttttca gtatgaaagt gttgcttttt24840caagtcagag tgggttaagg ggatgttgat ggcagtcatt tggcatggtg agtgacttac24900gcctactgtc cggtttaggt ggtaaaaatg gcagaaggcc ttggagtgcc aaaggcaagg24960gttatgggtc cagcagtacc atgaaaaagg gaatataggc tgggcgcggt ggctcacacc25020
tgtaatccca gcactttggg aggctgaggt gggtggatca cgaggtcagg agattgagac25080catcctggcc aacatggtga aacctactaa aaactactga aaatacaaaa attagctggg25140catggtggcg catgcctgta atcccagcta tttgggagcc tgatgcagga gaatcgcttg25200aaccagggag tcagaggttg cagttaactg agatcatgcc actgcattct agcctggcga25260cagagcaaga ctccgtctca aaaaaaaaaa aaaaaaaaaa aagaaagaaa aagggaacat25320atagaagatc tcaatctttt acgtaaggac attgaagtcc agggaagtct gatggctttg25380ttcaggctga gactatcgta gggtagactg acatctcagg ttgctgactg cccattctgt25440aatctctgca gagcacttgg ctgccacata gtataatctg aaaaactggc agctgccaga25500cgaatgactg aaggagtaag ctcaagtgca ctaggtgagg tcaggcctga aggtcattgg25560actgatactg aacctggcgt tttgacagga atcctattag aaatgccact tagtcacttg25620cctcctgact gcttcatggt aggaacagag gctcaaacta tggcctgaat tttttggatt25680aagtttaagt tttacttaat attatcccaa ttttatctca gacattatct caaaattatt25740tcaaaattta tggttgaaga gaggctgttg agtataaatt gctaatgtac tctgctgaat25800tccatcagct atgtgatttg actgagaaca gtacgatcct agcccactgg ggtctggtga25860attgcatctg ctcagtcaga gtgttccttg agctggaatt gaaatgcatg gccctttagt25920ttccagtgtg atgccaacac ttcaggcaat tgacttttgc tcttcagtct ccaatagggg25980gaaaattatg aaataaaatg gggataggcc aggcatcctc aagattcaaa cccataaata26040taaataactg taattaatca tatgcagtct ctcttgcctg ttggaaattt atactcagct26100gggtcaataa gcttcacaca gctcttaact tcctggcctg tagttggtgg gaaatgttct26160gggtgcgtca gcctttagag atgtgaccta gggttctttt ccaatatggg tccaaaatca26220gtgcaaatta taatgagttg tggggtgaga aaagtcacct ggaaacaacc ccagcccaag26280gggaaataga aatataaaaa agacttctgt tggcatttaa gaggctctct tagatgacac26340tgtctgttca ttgttgttag cctgttagac tgagtgagat gaacagtctt tcaagtatgt26400aagttcagta catatttaca ttcaagttta tccctaaagt atttgtttcc ctattataga26460atcttgtaga ttgcagttgg tatttttgcc tgttttctca tataatttgt taactctttc26520cccctctccc attgtgtcct tttttctata cttgcatcta ctcattatga acatggctaa26580tggtatccta atgaaaaagc aaccctctct ctctcctggg ggtcattggt gtcatttgaa26640tatgccaagg atagcatcct atcactagca ggtccctggc tgtgcctgtg cctataggag26700tagttgtgtt cctgcaggca agcactaaat tatcacatta tctctctcta tctctttttt26760tttttttttt tttttttttg agacaggatc tcactctgtt gcccagggtg gagtgcagtg26820gtgggatctc agctcgctgt agccttgacc ttccaggctc tacttttact cttattaccc26880taaaaggcat cctctatact gcagctaggg catttcttcc tttctttctg aaatataagt26940ttcatcgtat cattgtactg attaaaattc tccactgact tttttgtttg tttgtttttg27000agacagggtc tcactttgtc acccaggttg gtgtgatcgt ggctcactgt aggctccaac27060tcctgtgctc aagcaatcct cctgcctcag cctcctgagt ggctgggact ataggtgcat27120gttaccatgc ccagctcatt ttttctattt tttgtaggca cagggtctca ctatgttacc27180caggctagtc tcaaactcct gacctcaagc gatcctccca ccttggcctc cccaagtgct27240gggattacag gcatgagcca tcatgtccag cctaccccag ttatcctcta acctattatt27300tcatctttat tttctcctag taattctcac tttcaaaata cttaattttt atgtttgttt27360acatgtttat ttttgtcttt tccaatccat gagagtagag gaattcccag cccctggaac27420agtgctgggg atctgccagg tattcagtca atcccagttg aatgtatgtt acctgaacca27480atgctcctcc tcctgtcttc ttcacttggg taggtccttc acggatactt tcctgagtat27540ttgatggaac tcttgctttg gttatattct aagaaattac aattttaatc tttcctttgc27600tattggtatg ttttcaataa gccagttctc aaggaatttt cgagtttttt agaagcagca27660gatggatttt tttggtccat ttatctgcaa atatttcttt aaatgcagga gagcacagaa27720aaatatacta attgtttcta gcacagaagc agagagtgtt gccatgtttt ttcaagataa27780tgtggatcta ttcagatgag gtagaattga aataagaatt ttctttttct aaggcctgga27840ctctgtgcat gtacctggtt tgacattctt tttttctttt tcttttttct tttttgagat27900ggagttttgc tcttgttgcc caggctggag tccaatggcg tgatctcggc tcactgcaac27960ctccgcctcc cgggttcaag caattctcct gcctcagcct cccaagtagc tgggattcca28020ggcatgcaac accacacccg gatacatttt tttttttttt ttttttgtat ttttagtaga28080gacggggttt ctccatattg gtcgggctgg tctcaaactt ccaccctcag gtgatctgcc28140tgccttggct tcccaaagtg ctgggattac aggcatgagc caccgcacct agcctgacat28200tcttaactct atgtaagagg ctggggtagt ttgtttcatt cacagtgtgt acagcaaaat28260ccagatgttt tctagtaagc tccactaacc tgaaacacat aaaagaaggg ggtaaaaaga28320tccctgaaga gttagagcca gaacattgaa aaccaagtgt taaaattcta gtaagtagct28380cctttagcct tacttagagc tatttacttg tatttgaaac agttaaacta acttggctct28440catttttcta accacagtgg taacaatgac gagttaatgg gtgcagcaca ccaacatggc28500acatgtatac ttatgtaaca aacctgcatg ttgtgtacat atatcctaga acttaaagta28560ttaaaaaaga agtgagctca aagataaaca gggaagatta tcatcagcct gatagtttgg28620aagaagcagg aaaattgtag gaagcagaga gaggaagaat tcatacagga acagactggg28680acctgagacc actaagtaaa ggcattaata gtgttgtcac tcctattatt cctgaggcca28740tcaccatatc atagcatgta catatggatg gctgtaagtc gtcaataatt gaattcatgg28800ttaatattat ttaagaagcc aaagtgtgac ttacattctt gaaatatagt attttaatcc28860aaaagaatac agaatttttt ttttaactgt atactccact cagatgtaaa ctcttggaat28920gcagggattt ttttgtgtgt gttctttgtt tgctcagtgc ctgacacatg atggatgatc28980agtaaataat tattaaataa aaccatgaat gcaagtttta attatctaac agtgctaaat29040aaataggaca ttaagataat gccacattta atgcatgagg gttgtttccc cttcttcatc29100tattgaatga agaaaaacca taaagtaata aacttttaaa gttaaaatag ctttggacat29160tttgaaggct agctcatata tagttggagt gaggatcagc aagttcaagt gatttgcctg29220aaatcgcaga gcaagttagt ggaagagaca gaattacaag cgtgtctttc cttccagaat29280accccctgat gattttcttt tttaatttag agttttggga atacaacata gagaattata29340tatttttggg aaagactata gagttctttt ttgaaaaaaa gatcaatgac agcttcaaaa29400ggaatattga gtcgaaccct ttattttctc tatttatgct atttaaattt tagacctgtc29460acctctgtgc tattagctct gtagtgttga taccaaacat cttatgcaga ataactagac29520tatagtactt gaatgctttt catgagggtt tgtgctagcc cttttaaaga agaaaagaaa29580tatctttttc tttagtttgg aaaggatgcg ttattcaact gaggagagta agatcttatg29640accaagatca ttttagccaa aatgactgag atagaaatac agctttcctc attatttttg29700cataggtgaa ggaaaatgta gtatattttc ttcacttttt ttggagtggg gatggagcat29760ttaggaaata cattgttttc acttacttta aatgggaatt ttgttagcag tatgaattac29820ttcaatctgt ttctctcatc tgattcttat ttccttgtga gtcagacatt tttaggaagt29880gtaatcagta agctaaatat tctaatacat gattattttc actacagtac gtctcaggtt29940tcctttttca tatatgcatt gtatctgtac tttggctctt tgttgtagtt ccatttatac30000ttgtggatta ttagatgtac agaggagtaa attttaaatg taaatttcaa caggttacag30060gagaaaaaag actgcaggat ggaaaaacta gtcagctttt ttcctaggga tcttctctct30120gttaaggaaa cagagtgact ggtcttccca ccacagcgca cttcttgatc tcttataact30180ctcagtcacc acttatacat taaatatatg tgtagtgatg tttataatag agctgtattc30240aaattgagaa ctttctttta caatgaactg aacaataact gaagttattg tgctataact30300aattattatt aaaaattcag tcataattta aaaaaataag ctcaggattt cttaaaccat30360cttcaacaga tacttcccca aatatatttc taacggcata taatgcagtt gcaacaggaa30420
cttttgaaag aattggtaca ttttcttttc tttttttttt tgagatggag tcttgctttg30480ttgcccaggc tggtgtgcag tggcatgatc ttggctcact aaagcctcca cctcctgggt30540tcaagtgatt ctcctgcctc agcctcctga gtagctggga ctacaggcac ccatcacaac30600gcctggctaa tttttgtagt tttagtagag atggggcttc accatgttgg ccaggctggt30660ctcgaactcc tgacctcaag tgatccaccc gccacggcct cccagagtgc tgggattaca30720ggcatgagcc accgtgcccg gcctggactt tgttttctta caaaacattt tacagtatgt30780attcaacagc catgattgta gttactctgt aaggctttgt tagttttaat aaattttgag30840ggaaggcttg atatgtttta atcttaagac agacatttaa aactctttga tttgcagctt30900attttatagc aaggtaaagg actttttctt ttcttttctt tctttctttc tttctttttt30960tttttttttt ttgagacagg atctcattct atcacctgtg ctggagtgca gtggtgccct31020catagctcac tgcagccttg aattcctggg ttcaaacaat cctcccactt cagtctcctg31080agtaactggt actacaggca tgtgccatca tgcccagcta attaaaaaga ttttttctgt31140agagacaggg tctcgcttcc caggttggtc ttgaactcct ggcctcaagg ggtcctcctg31200cctgggtttc tctgagtctg ggattataga tgttagccac ccaccactcc aggccttttt31260cttgttttga ttactgaagt agattctgta acagcatgta aaatgtaaaa agagcgttta31320tgctgccaaa ggaatgtcaa acattatctt aacccaataa agggttagta aaggggcgga31380aagactgaaa gaagcacagg actagtatgg tctgtttgac taatgaactt cttttgaatg31440ttatcaatga atgtgtttca ttgataacat tcctgcctcc tcaaggcaga tttaaagttt31500ttctccctgt tgtgctatct ccttttcatg ctaagtcaac catttcttgt tagttgatat31560aaatgcaagt ttgtttaatt ctgtactagg catgttatct actcagctcc ttaacctgac31620ttgtgttttt acaaggggtt ggcatttttg tgaaggaatg cttcaagttt tcacttggga31680gggacatttt attttggggc tctcagggtt accctcaatt atgtatatgg taagcaaaga31740aggcagagat agattttaat ggaatattaa agataggatt gtacgacttt aaacacaaat31800ccgtgaatat atctggagtg atacagagta tatattaact ttgcttttaa aatgaattta31860aaatggaata caaattcact tgcgtgcact aactatagtt ctttatgact tttatagcaa31920ttgtatggaa agcaaacata atagaaacta ttctattatg aattaagctg tggttatttg31980catttaatga atataatgag cttcattgat ttcttataat tctatagtga acctcatgaa32040ataaagaaaa agggtcaaat ttgtgactat agttccagtg tgtattttag aaatggtaga32100aagcataagt atgttatatg gcaattgcct tggaaatggc atatattttg taaaaaatcg32160ggtcttaaat aatgcatcac tactagagtg caaacgtgta tccttgtgtt cctctaaggc32220tccctccctt tcaatgcatt ttttgtttta gaggactttg tgaaataaat gctcgattaa32280cagttatgac attattaaac ttaacccccc atcacacatc agaaatccca tgtaataatg32340tttgcaacaa attttttata aacagagact tttaactggc tcacttaaaa tagttattga32400ccttattttc tccttctttt gtacctttta cttgcatttt ttgaactctg cccctgaaac32460tagctcaact cgtttcttag ccagcttctc ctgtcaagct tccaggctcc tgccctcctt32520tgaggctgag aagtggtcct gggccaacct cgttctcact gttggtttgc ttctaaaatg32580atataattga cataaaggtg acccagggag actcagaatt ttctagcttt acactatgtg32640aattgatata aatatagtgt ttattttctt tcgctttctc tttgtttcat gatttttttt32700ttttctcatt cttctttatg gaaatactct tcctctgcta aaattgttta ctttctcttg32760ggctggggtg ggcccttctg gcagtaattg cctggggaaa taaatggatt ggaagtgaaa32820gaacctgccc ttcttggaag caggctggcc ttttgatagg gtgtggaagt cagggacaaa32880gctagagtct ggatgccttc ccagcaatgt gtgagtgtgt tgctttagct tcttccacca32940cagtccaaaa atttgtgtat ttagaacctg aggattatgt ttcacacatt tctgttgact33000tttacttggt tttttgattt atcttgcgtt atcaggataa tgcttttctc agtgacttcg33060gaaaagcctt tttgtttgtt gtttgatatc ctgttcagtg tatatattct ttacaactat33120aggttgttat tgttgtgttt gtgtgttttt gcttttgata taatagagaa aaggctaaag33180aaggtgacgg aaggataaaa gtgtcttctg ttttcttagg acttgataca ttcacttctt33240tccataggga ttgtcttttg tgaagaaccc ttgggtcaat ttcctgtcca gtcttggcct33300aaatcctttt taaaagcaac aacaacaaaa gaggagaatt caccttcagt ttacagaata33360gacctcctct ctgtttccag agaacaggct aatgtgcctc ttctgtttga ctgttttgtt33420catttaggtc tgatagcttt caaaaagtac acttgagggc tctctgtgtg tttctgtagg33480aatcacatgt gtaagataca ggtgtgtaag tccttctctg cttggctggg gggaatgcgt33540ttgcaggcag agtactaatc atgatcccca cagtaaagta aaacttgggg aaagagtagt33600acatggtatg ctttgattgt tacttgtctg agtaagcact gtatcagcat atgtttggtg33660tgtctgaact atgagatacc tccttcaagg gaattgtaca aaacatttgt tatcacatta33720attggaataa taaaaggttt gcttgctaaa acacatctag ttcaagccac ccagtgatct33780tgacttttat agttaaaagg gtgctgctgg gagggaagag gcttttgagc tcactccttc33840ttctgtttta ctgtggactt gctgtcaagc tatccatgat tacagacccg tttgctagga33900ccttctctga tgaggttcta tatgcttatt ttgtaagact catgcacttt tcttcaataa33960gtcactggat ttgtatgcga gtgtgaggcc tggccctctg agttgtgcct tccatgggtg34020tgacagtgtg acacgactca aaatagttga aggagtgggt tttgcatgca cagctacagg34080agcaggtggc tgcacagatg aagtgtaagg agcatttgct acctttggac cacaaaaggc34140ttaggtaaag ggcttagtga ttaaagggct acctctaggt tacatcttgg ggtttttatt34200taatgtttga aatgccttgt acatctggat ttctcagtgt aggtgattgg aagagcatgg34260atcgctttta tttattttta atttcttttt tctttttaat ttttctgggt acatagtatg34320tatacatatt tttgggtaca tgagataatt tgctacaggc atgcaatgag taacagtaat34380atcagggtaa atgggtatcc attaccttaa gcatttattt ctttgtgtta cgaacattct34440cttagttatt tttaaatgta cagtaagtta ttgttgactg cagtcaccct gttctatcaa34500cttccagatc ttattcattc taactatgtt tttatactca ttaaccatcc ccaccccaac34560ctcactacca tctccaacct ttggtagcca tcctctactc tctgactctc tgtctccatg34620agttcatttg tttttatttt tagctcccac aaatgagtga gaatatgcaa aatttgtcct34680tctgtgcctt atttatttca cttaacatga tgtcctccag ttttacccgt gttgttgcaa34740atgaaatgat ctcattcttt tcttttttga gacggagtct ctcgctctgt cgcccaggct34800ggagtgcagt ggcgtgatct cagctcactg caacctccac ctcccaggtt cacgtgattt34860tcctcctgtc tcagcctcct gagtatctgg gattacaggc acccaccacc acgcctggct34920aatttttttg tatttttagt agagacggag tttcaccatg ttggccaggc tggtttcgaa34980cttcggaact caagtgatct gcccaccttg gcatcccaaa gtgctaggat tacaggtgtg35040agccactgtg cccggcctga tctcattctt ttttatggct gaatactact tcattgtgta35100tatgtaccac atttttgtta tccatttgtc tgttgatgga cacttaggtt gattgaaaat35160cttggctact gtgactagtg ctgcaataaa catgggagtg caatatggga tgcctttatg35220tcttgctgga gaatgcatgc cccgaggaca gcaaggctgg tcgttgaaat tactcttctg35280taattgatct ttgagctgta ctctccgccc ctccattggt catgcttgta agggatggag35340agaaatatgc ttacttctat gctggcataa gaatcaaaac cctgagaagt tctaaactaa35400tggagagcat ggggattaga aaggtaattg aggtttctat ataagaaacc agaaagatag35460gagagttttt gtaaacatga aggtgttaca ggaagggggt ccccatccag atgcctagag35520agggttcttg gatctcacac aagagttaag ggtgagtcca taaagtgaaa gtgaaggaat35580aaaagaatga ctactccata cacacagggc tgctggttgc ccatttttat ggttatttct35640tgattatatg ctaaacaagg ggtggcttat tttacccagc tcctattcaa gatggagttc35700ctctggttca cacgcctctg acaaagggac ccagggctta ttccacagag gagtgacaaa35760tgtatttgca tggaatcttt ttctttttct ttttcttttt tttttaagac agggttttgc35820
tctgtcgccc aggctggagt gcagtggtat gatcatggct cattgaagcc ttgacctcct35880gggcacacat gattcctccc actttggcct cttgaatagc tgaaactaga ggtgtgcgcc35940accatgccca gctaattttt aaattttctt taatagagat ggagtcttgc tttgttgccc36000aagctggtct tgaactcctg atatcaaaga atcctcctgc cttggcctcc caaattgctg36060ggattacagg tataagccac tggtccagcc catttgcatg attcttactt atgctaaatt36120tgtattttga taagtaaggc ttctggaaga gagaagacag ggagatgagt aggagagtga36180gtacttttta aagagatttc agaggccagg aagtgcgatc tggttctgga ataaatttgc36240ttgtgcatag ttgccttgcc tatgaatggg aggaggtaga agtggtggat gcactcaggg36300acttacatga aataggatgt gaggagttgt tctttgttaa aagtcactgt ctataatggg36360agccctacaa ggacatctaa gaattcatct gagccagata agttgcagca gtcaggtaca36420aacaaaggtc agcaaacttt gagaagtggc aaatttagtt tttgtttgct agcagtaagt36480acattttgtt gtgtagcaat tacatctgtc agttctaggc aaaattttcc caagtaccta36540ttactcttgg gagttttaga cccaaatatt catctgtgct gttttctgag tagatgttcc36600gtcaagatct caaattttct tagttgcaag ctgagcttct cttcttcctt aaacctgctt36660ctctttctag atcttaatat tgaaatcttg gggtcatttt ccagctgccc aaactaaaac36720cacttattgt gttcagtctc ttcctgctgc ctcctgtaat cggttgtcag gtcttgtcat36780gctgtctaat tgagtgctgt ccttagacag ttgtgcaaaa catcagtctc tagatttgca36840ctctttaatg ttgccttaaa ccaggctctt ctctcacatg ggatctattt cattgccctc36900taattggtct ctctgtcttc gtaagcttca acactaaaaa tctacttgaa cagtctttac36960agaacgttag acaaaacatc ttacttcctt aatctacagc ctctactccc cgttgccaac37020aggatatcat agaaatattt tacttcacgt gtacacagtc ttcctctctc ttccctctgg37080acttcttttt aaacaccatt tctagctgta catcatctcc agccaaacga gataactatt37140tgtatgccta gaacgtacca tgatgctccc ctttctccac caattgtctt tctctataac37200gttccctact cactcccctc caaatttttt ccccttcaag acccttctcc aaaatcctgt37260cttcactgaa gccttcttta attcttttag tcagagtcaa tttcttgcaa cattaaaatg37320atatagcacc ttgctttttt tttctatcag actataacac tcagtatagc acaactagtt37380ataagacaga gtgcagagtt acaagacaca ctttgatatt taatcctaca gatagattaa37440ttgcttgtgg gcagttagtt gataaactca cagatatatt tagttgagca ccatttgttt37500aggttttgcc aactgagctg gtgttctcct tgcaagaatt agagctagct ctggtctatg37560tcaatgcagc agctggtcct gagtaagcat atatgtgatt taattaaaag gctataaaac37620atggatttga agctatgata aataagctaa ttctattttt taaaattttt tcacctctgg37680tttccttgaa catttattgc agaagttagt aaagatttgt gtatattttt ggtccccttc37740tttgctagtt ttctagtaaa gctgttactt agaaggaaat aatctttttt ttttgtttgt37800aatcattgtg aaaacgtttc tccttacaat ggtaaatggg tacatgttgt ccacagcatt37860tggtttatat agttctaaat ttcattcttt cagcattaaa acaaagttct tttttaaatt37920ttttattttt tgagacaggg tcttactctg tcacccagac tggagtgcag tggcgcagtc37980tcggctcact gcagtctccg cctccgaggc tcaagggatt ctcttgcctc aggctcccga38040gtagctggga ttacaggcgc atgccactac tgcctggcta atttttgtat ttttagtaga38100gacaaggttt cagcatgttg gccaggctgg tcttgaactc ctgacctcaa atgatctacc38160cacctcagcc tcccaaagtg ctgggattac agatgtgagc caccgtgccc ggccctaaaa38220caaaggtctt acattacctg gcagaagatt actagtagat actttgctag agacttcttc38280attgccttgg agattaaccc aaatgccaag ggactgaaca tttgggtgac tttgagaata38340gggccttctt ttgggagtga caacaaaata tggtatagct tttggggaga agttgccttt38400tgagaccaaa ggcaggacag aaccaccagg gctcaagaac ataaactcag tcttgttttc38460tcacttagta ggcagtgact ttggggaaat cccccatttc ctctgagcta ccactgcccc38520ttttatgaaa ttagatgttg tgagggtgaa atgagctgat gtgtataaag aattaaaagc38580acagtacctg ttacctagta aataataaat ggtggtgatt attattgtaa cattcataac38640aataacaaca ctcataatga taatcttagg aaagtcaaat gaaactggct gattcttgcc38700tttgactttg agctagccag atgaccgtac agaaggattt gatcacagta gaaataatga38760gccctcaagc atacattatt tttttaaatt atggaaaaag gaaaactctt tattgattat38820agttgatatt attgctaact ctgcaagatg acatgagaat gtagtttttt gatagtatta38880gtggctcatt gggaaaatgc atgaaaatat cttcctttgc aagttttggt ctctgacaaa38940tcaaattaaa acaatactat caggtaggca gggaacatag ggacccacac atttggaaat39000actagcttca aacttaactg gctgaatgaa cacctctgag aatgaaacat tttcattgac39060tgtgttgtat ataacttccc tgagttggcc atgttgatgt gtggtatcct ttgtgaggtc39120aacaatgaca ttatcaacaa gacagaagca atgataagtc aatcagtcac aacactcata39180tcctagggac caccacactg ctatgagtca ggcatgtgcg ttgtttaagg taaatagtaa39240atagaactag gactttattc tctgtctcca gttgtcagag gaacccagtt agccttgaga39300tagcatgcta tctcttggct gctagggctt acattgttgt gtgataggca cccatgtgaa39360tgtttacaac agtacattga ttggtgatag tgagctatga caaatgggta cctgctaagt39420tagggttttt actatcccag caacctagga tataaggttt tgcctagggt tgactgaatg39480atgaatggtg gcccctagcc agcatacacc aactttcctg cataatttgg gaaagagaag39540atggttgata tagcaaaggg acagtaaaga agagacctcc agcagcagag tacatatgct39600tgtgaaatat tttcctagta agaatcatta aaattttttt tagagacaga gtcttgttct39660gtctcccagg ctggagtgca gtggcacaat tgcagctcac tacaagctca aactcctggg39720ctctaatcct gggaagaatc ttaattaaaa agataaagtt tagaatttag gagtttgtca39780ggaagaatga gatcagaaga aaaaaatttt aaaaatgcca ccacatatca gtatataatt39840tatagtatcc taaaatgagt tttctatagg atcatagtta tcaactatat tgcaattctt39900tatttaggat agtatgataa tagatacagt ttattcagta tggacccaaa tatttaaccg39960tatcataatt tatgttgaga tactgttttt ttagtatcct aagttaaaac taccttcata40020ataaattgtt ttcttgagtt gtgagctatc agtagaatgt aacgtgtgtt gtccagacag40080gccagtgctc cacccctaca aggaaatagg gtagtggctt ccctgtttcc ctgcttttgt40140gaatgtaaag tcttaggcag gtcaaattac tcaactctag aatttttttt agttgatcac40200acaggattat ctttttacgt ttaacagtaa aagcggacaa aaggattttt gatggaattt40260tcatttttat gcaatcttct tagtttctga aagagttaaa gaataatttt taagtcctca40320tcaaaacttt agaaaattcc attatcagtc cagtagtgga aaacaagtga gaggaaagaa40380agttaagagc taagacaatg cagaacttga gaaatattca gtggagcaga gtgtggggtg40440gctcatgttt gtaatcctag cactttggga agccaaggta ggagattgct tgagtccagt40500ttgggcaaca tagcaagacc ttgtctctac agaaaaaaaa aaaagaagaa gaaagaaaga40560aaaaaaaata gccatgcatg gcggcatgca cttggtagta tcagctactt gggagaatca40620cttgcccagg agtttgattt tatagtttgc tatgattgag ccactgcact ctggcctggg40680tgacagagcc agactcagtc tctacaaaaa gagagaaaga aaaaaatata aatacagaga40740gcacaaatat acagtgagtg cttgatctgg agggttttct cttaaaattt tggtgtcatg40800caactctggc tagaagaaaa ctttttctgt taagttcccc taatgaaagg aattacagca40860ggttaaattg gtcagtttgt gaaagtctta aggcataata cctctgactt cttatctgta40920aagtatactc tttttttttg agacggagtc tagctctgtc acccaggttg gagtgcagtg40980gcatgatctc ggctcactgc aacctctgcc tcctgggttc aggcgattct cctgcctcag41040cctcccgagt agctgggatc acaggcatcc accaccacgg cctcccaaag tgctgggatt41100acaagtgtga gccaccacgc ccagccgact ctttcaataa tagaaatata acactgctgg41160cttttggctg tagtgaaaac ttcattagaa agggaaaaac ttgggaaggg agtcttacca41220
caatttgaat taaacacttg ccaaagaacc tgaagcaagc tgatgtttat tgtcttgttg41280agtgacccaa gacttctttc tgctggttct ccttgaggag aggattaggt gttgagcaaa41340tttacaagta tagcatgcag ttttgttaaa tacccaatga catttctttt cacagttctt41400ttgactttat actgtttaaa tatcctcagt tatctcttaa tttgcttttc tttctttaat41460tcctgtcgaa ccagaaagta acttttatgt gatcaattcc cttgtctaga attttttggc41520tatttatttt gagttgcagt atacacattg gtttgttagt aatttcctta ttttatgttt41580tcttttctag tttaacatgg tttcataatc tacacagtgt cttattattg ggatggttat41640gcgataacta tcccaattaa cttctttttg tttcttattt ttctttgtag gctaagaaat41700ggcatttcaa aaggcagtga aagggacgat tcttgttgga ggaggtgctc ttgcaactgt41760tttaggactt tctcagtttg ctcattacag aaggaaacaa gtaagtaact gtacttgtgt41820tgattacagt atgcaactta aatctgctaa agagctgtct atggggaatg tgtgcactaa41880cggttttgtt tagaaattac ttgtgaagtt tctcagtttg atctgaaaga tactaattta41940gaacagtgcc ttaatgatcc tggagaactg tgtgtatctc cttttttaaa aagtgaattt42000ctttttcagg aagcattatt tagcctgtgt tttggctctt tggtttttaa aagactgctt42060catttataag tgccaggatt tcttcattta gactttcctt gtctaattca tcgaaatgtc42120tggatacctt ctatgttcct gccaaaagtt tgtcttatta gaaggcaggc agctgagaaa42180ggaaatggaa aagaaaaggc ccaactgaat cctggtttta tttcttcaag gtttagttac42240ttatgaagta ttactttgtg ttaggtaaaa atttaaatga aggaaagcca ggaaaatgag42300attggtaaga agtttccaaa tattatgaag tgttcagttt gttttctttt tttttctctt42360taccttacaa ctgtgacttc ccatagttca aactgatgcc cggaaataat tttgctgaat42420ggctttgttc ttttcaggct tgaaatgctg gcaaccaccg tagtagttag tatcagtctc42480ttgttccccc atcccatgtc attaggacat tttgtgatac tagctgtgta gtgcatttct42540ttctcaaaga tgaaggtgaa ggaatttggc aaacaatttg taactgagtg attcattttt42600cacatttttg tggctgcctt taagccatgg tttgtcttat acttcttgtt tctgtttctt42660tgtgctacaa ggtggtggag ccaggcgcat tgcttgcaat cccatatcct gaatgaggat42720cactttaaaa acacatatta tggccaggtt cactgactca aacctgtaat cccagcactt42780tgggaggcca gggcaggcag atcccttgag cccagaagtt tgagaccagc ctggacaata42840tggtgaaacc ctgtctctac aaaaaaatac aaaaattagc tgggcgtgat ggtgtacatc42900tgtagtccca gctactctgg aggctgaggt gggaggactg cttgagcctg gaaggtggag42960gctgtagtga gctgagatca tgccactgta cttcagcctg ggtgacaaaa tgaaaccctg43020tctcaatcaa tcaataaaat gcataaataa aatcacatat taggaagaat tagtatgctg43080gcctatcatt aaaaaaccca acaagccaat ttttatgtga ttcttgctaa agatgggtga43140taccgaaagt cttttgatta gcaatagtct tttgattagt gttagtcttt ttctgcattc43200tgaactgtga ggacatcact ctcaatgcat gtatgttgca gtttttattt tttgaattga43260cacataatca ttatacatat tgattgggtt catagtggag ttttgatacg tacaatggta43320ttattaattc actgacttgg tattgaaact cttcctcatc cattgatcat gcttgatgta43380ctatttcttt taaaagattg ccttggttcc ttaaaagcaa gtgtgaaaag atgtggcttc43440tgctgaggct atttcagaag tgcacttctg tttgggacat gggcattaag ggattagatt43500acttctgcca tctttactgg tgctatctga aatgatactg aaaaatgccg atgccaaagg43560tcacaatatc taatatgatg agttgttgct aaatttatat gtggtttata tcttttaacc43620acagtaagca tgttctactt cagagtttat agcttaaatt tgtacctagg taacactgtt43680ttatcccttt ttttggtttg atttctctct aataattgat cttaaagaat agaataaaca43740gaagagactc tggccccaag cagtcaagtt ataggtggat tttattcagt aaaatcaagg43800caaaataaag acctattgat tcctgggaca ttttgtcatt ttatctttcc tgagttagac43860atcctgacca ccctaatagc caaatgaaat ggatagcagc agcttgaagt catttataat43920tttaacctga caagttctgt tgaaaaagat tactacaggc atatgcaatc aatgagttgc43980ataagaaatc caactatgtg catttctttt ctggtgattc ctgtcccttt aagatactgc44040cattagcatg tatttggggg gtagcaggtg cgatgtagcc atagggactg gcaaaaaata44100ataataataa taaaaaataa aagcaagaat gagaagagga aacaacctat tccagacatt44160gacataaaat gaccacaggc ctccattaac tcagctgtcc tctctcagaa aatatctgcc44220aataagagcc attctagcgg gggggaattt tcctgacttc aatagtcaaa aaaaaatctg44280ccctttgaga tacattaatt ggctacactt ttgtttgtag aagctggggc cttaaatgtg44340actaggtatg ctccattttt ctgcagagtg atgtttaagt attggcttgt tacaggtata44400tttacctact gtatttttgt attttttcta gtttcctaag aagattccaa ctgataaact44460gccgtttatt aagaataagt tagttttgaa accccaagtt cctgatgtcc tagagagcca44520cctattgact ttggttctgg cacattttgc tgtgttcttg catgctctgt cttcctggcc44580cctggcttgt gccatttggt actcccagga caaagcttct tccctgaaat aaaagcaggg44640gctatattct aaatacaaat ggattgaata taaaagttgg tacattgtta aaacattttt44700ggtgatgaga tagtactatg agtgtttagg tgccaggtca gctcctgaaa acctttgagt44760caattctgta gttaaattac cttatatatg taaagaaaac aattgtatat tctttaaaaa44820ttaattaaac ataagacaga ttgaaattga ataagatgtg gtttcactta cctttaatgc44880agttgcctga atcagagcca ttgtaggtgg gagaatagag gcctccatgg agactggtgt44940ttctgggagc aggttagggg ttagaggtgg tagcaatgat gcttttgtct cactctactt45000catagtatgg cttttcttca tacaagcata ttaacaatgc tattacttag gtttatcaat45060gatgaatagt gcccactgtt gagccaagtg aactctagag gtttattatt tttattttct45120aagggaaaca ggtcagattt agaagagata gaggaatgtg agggttgtta tagcctaagg45180agagggcaat tattattatt attcttttgg agctgagcag cctaaatctt caattccaac45240caaggaagtt gatgaggaaa ggagatgaag gttggctgac ttcttttgct tgtgtccacc45300tgcagaggtt tgtggccaca gataacatcc acattccagg ccttcctcag ttaagcatct45360gagggctttt agcgtggtct tagagtggct tcttcttcat tttcttgtcc ccttttcttt45420attttccacc cataagcagg tgacagtttc cacaaaggga gcttgagttt tcttctcccc45480agaccagctg tatgccaagg aacaagagtc ttagtactgc gatataagca ttttaccact45540caaagaaacc aaccccactg ggaatggagc aagcagagac actctgtggt ggtgtggaga45600ttttttagat caagttgatt gtgattcact atttagtata ggttggttta ataatacact45660tttaattgga tgaaacttgg aggtcatctt atccagtcta aggtctactg aaaggaagta45720gcacttcata gaggagtctc ctttttctaa ttccaggctg gtgggagaat ggagtagatg45780aagagggaaa tttaaattag gaaaaggggt catgggatac ttttagaaca agtaaacctt45840tttgagcaac agcagtagag agcattaaag tcagggctcc atgaggagtc ctcaggaaga45900tgggaaggtt gggatgggag tggccaggga gaatgaaaca agaatagagt gggaaggagt45960acagggattt cacacggggc taccctagtc ttttttcttt tcattatagc ccaacggcac46020tgtttaacac tttttttttt aagttaacat ttatttcata ccttctaggt cctaactgga46080aaaactgatg tgatgatgta ggcttttctt taacctcagt gatgtggttg atatgacaaa46140aatctattcg ggtcagaaga gtggtctttc tcacataaca cattctaggt ttgttccttc46200catgacctgg tctgaaggtg tggtggcttt gggctttgtg agccgaggta aaacttgaga46260accactgaga gctttctcaa agcccggatg tgaaaatttt tttcccccct gcaccataaa46320taggcaagga gtgagcttca gaatatgaaa atcaagatct ctctctcttt tttttttttt46380ttttcctgag atggagtttc actcttgttg cccaggctgg agtgccaggg gcatgatctc46440agctcattgc aacctctgcc tcctgggttc aagcgattct cctgcctcag cctcccaagt46500agctgggatt acaagcaccc tgattctaag aagatcattc ctcctaaggt ctctttttaa46560tgggaggaat tatcttctta gaatcaggga agtaagaaat tctcattgat tggtgtgcca46620
ttcataagca acggaacaaa aaggccaaga gcgttatctt tagagcagtc tctgcagact46680gcctgcatct aacacctagc tgtacttttc ttttttgagg tatgtttcct tacctttatt46740tatttacata tttattcctt tatttttgtt tgacatgtca taattgtaca tatttatgga46800atgcagaatg atatttctgt gcatgtatac aacatgtaac gatcaaatca gggtaattag46860catatccatt acctcaaaca ttgatcattt ctttgtgttg tgaacattaa aaatcctctc46920ttgtagcttt ttgaaaatat acactaaatt attgttaacc atattcagca tatagtgtta46980caaagcccta gaacttattc catctagctg taactttgtg tgtattaacc aacctctccc47040tgtcttcccc ctcccctccc ctttccagct tttaatacca acagttctac tctctatttc47100tatgagctca atttatttta gctcctagat atgagtcaga acatgaagta tttgtctttc47160tgtgcctgac ttatttcact taacataatg tcctccaggc tcatctgtgt tgttgcaaat47220gacaggattt cattcttttt cttttttttt tttttaaata gagatggggt cttgccatgt47280tgctcaggct ggtcttgaac tcctgagctc aagttcaccc acctcggccc caaagtgctg47340gggatcacag acgtgagtca ttgtgcccgg cctatttcat tctttttaat aactgaatag47400tattccatca tgtatacata taccacattt tctttattca gtcatctgtt gatggacatt47460taggttgatt ccatatcttg gctattgtga acagctagtt ctgtatcttt atagctgagt47520gactataggt gattttctta aattctctca gcctcatttt ctcccttcta tgaaataggg47580ataacagtgg tacttcctgc acaggtttat taaaaagcag tgaagcctgg gagagttcct47640ggaacatagg aagctttcaa aaagtgttac ctcttatgag attcccaagg gatatgaata47700taaagtgcgg ttgtagtcag caagcaagtt gctcttgtca ctagagatct tgcattcacg47760gatactttgt taaaactttt gggattagta gcaaatgata tatccttcat tttaggttat47820agaagaatgg ctcaaagaca ggtgtaccaa cactcttctg ttttcctttc tccttgcttc47880cggatctgag gatgagcaag aatatcaaca ccaattgttg acttaacctc tctaactgtt47940ataataagtt ccttgaattt cccactgttt ctttcttttt ttctatgtag gcttatatga48000attatggcat acttgattta tataactgat atgttgctaa aaagttacaa gaatatagtt48060cctttgataa attaatatca gtgccattta ttggaattct gtggtgaaac attctttaaa48120aacaggcaat attagggaaa aaagctctaa ctttttttgg gggggataaa tctgaatttt48180catatatcat tgtagcttga aatggaatgc aattaaacta atttcttctt tcccaaagtc48240agtgatgttc gagcatgttg atattataat aggtgttgat gacaggatct tcatgatttg48300caaaatagcc tctcactttg taagagacat caattctcta tgcttgtgag tggcttagga48360aatactatag ttcactctgt tatatgtgtt aattaaggga atatgaaaaa aaaccctgat48420aacagtgatg tgttaggtga gttgaaatac aggtaagcct atactacctt tgttagtgtc48480agcaaaaccc aggaggaaaa atatttatgc taatgagagg caaagtgatg catggataat48540taaaatcagc aggaattttg agaacgttgt tttaacttgt aattgcaatc tctccctctc48600cataccttat ttgtaagagc tctaacgaag tagtgctttt tgattagtag taccaatgac48660tcaaaagttt taaaggcagt agcaaaagat aagattggac aagaagcaaa aaacaaaaaa48720aaaacaaaaa acaaaaataa caaaaaagaa tgatcaactc tagtcaaaat aagatctaat48780ttaggattta tttgaataga ccccaaataa agcaacattt agcttcctga atgtgtctgt48840tatgtgaatg tggctgggtt ttggtgaacc tgttgtagcg tgtgtaggtt ataaaaaatt48900tcagaattcc aatttcttca taaaataaag ataatgtaac tttacttaca ggcttttttc48960tctgccaagc actaagtcaa tacatacaaa tcagtgatca cagtacctgg cacatagcag49020gtcttcagta tctgctttac ttctttccct tcccctgagg taggatatga acatttgaaa49080tagacctagc cttgtcaggc ataataagaa agatataaca tcagagttta aaaaaaaata49140gctgaacttt tggttggcac acttttgctt atattgatga ctaaacagat aaactggtag49200agggtagaga aacttgaagt tcaaatgagt atttgttgaa gagaaatcaa aatcataagc49260atagtatcta agatatgatg tcagttagga aacaatttaa tcttgtggca tctgcaggaa49320aaaaagaaaa taaagaaaca atttaaatta caaacattcc accttagcat tgcttttcag49380cagtttcttt gtttgaatat aacaatttgt ttatcttttg aacaccctta acattttcga49440tcatttaacc tcaattagaa acattcttca ggttcttgaa ctaaggagga tctgtgggtt49500tgaaatcagt gtaactcttc ctgaacgttg atgtagctgt agctgaaact atcacatgac49560acatttattt gcttgcttgc tttttttttt tttttttttt ttttaccaga ttgcttttaa49620ctttgtaagt caattcaggc tactagactt tcttgttctc ccagagtaaa caatattcta49680tctgcagagg cattttgcac aggtttgtaa gcaatctttc cactcccctc cccgactttt49740tttttgagac agtgtctcac tgtgtcaccc aggctggagt gcagtggcgg gatctcggct49800cactgcaacc tctgcctccc gggttcaagc aattcttctt gcctcagccc cccaactagc49860tgggattaca gtcgcctgcc accacggccg gctaattttt gtatttttta gtagagatgg49920ggtttcgcca tgttggccag gctggtcttg aacgcctgac ctagggtgat ccgcccgcct49980cggcctccca aagagccggg attacaggcg tgagcccagt cccaatcttt ctctttaaaa50040aaaaccttag gaaatgctat aaaaacgaat gttcctaaat agtctttgtt ttttttcctg50100tacctgttga tggcagaata atgttgcttt ttaaaaattc tttccttgat ggccagttga50160ggtggctcat gcctgtaatc ccagcacttt gggaggctga ggcaggtgga tcacctgaag50220tcagaagttc gagaccagct tggccaacat ggcaaagccc catctctact aaaaatacaa50280aaattagccg ggcatggtgg tggatgccta taatcccagc tactcaggag gctgagacag50340gagaattgct tgaacctggg aggtggaggt tgtagtgagc cgagatcgca ccactgcact50400ccagcctggg caacacagtg agactgcatc tcaaaacaaa caaataaaca aacaaaaaca50460aaacccaatt ctttccttga gcataactga aactctgtac cccttgttca acatctgctc50520acccaccctc tgcttaccac tattttactc tctgcttcta tgagttcgac tattttaact50580ctcatgtata agtgagatca tacagtattt gtctttcagt gtctggctta tttcacttag50640cataatgtcc tccagggcta tccatgttgt cagcaatgtc aggacttcct tcttcttctt50700taaggctgaa taatatacca ttttatgttt atactacatt tctttattca tccatctgtt50760taggttgttt ccatatcttg gctgttgtga atacattctg cataggatat ctaacactgt50820agttcccacc taaatgttaa aatcaaataa aatggccatt tctgatggtg gaagaaggaa50880ctcatggttg agtcataagc tacctgtttt gcttaaagag ccatcagcgt tatgtcaaat50940gtgaaggtag aaatgtctct gcaccatggc tttctagggc ttgcatttga tgatctgtcc51000ttctgaggtg ttgtctgtat ggatttattt cctaattttt atttttgtaa atgatcatgc51060ctcatccatt catagtccat tctctattgt gtatggactg tatgcaatgt aggcgagttg51120taaatcttgg gttgtaaaac taagcacatg tcaaacttgt gtattaattg aagtaagcta51180attggtataa taaccaatcc tcaaagctca gtggcttatc ataatgcacg tttatttgtt51240gctcacatta cagttcagtg tggataggtg gggaacctat ttcctgcaat tacccaggaa51300cctatgtctc tttattctag tgggtctgcc atgctatagg ggtctcaccc tactggatca51360tttctactcc actgtatggg agtgttctct ggaagaaaag aaaaagtttt gttaagcatt51420tagccattac atatgtgtct tgaatttagt ttctaatctg gtggacaaat gagctatatt51480tcttttgatg agaattttat tgttagggtt atgccaaaga ctgtatcatt catttgcctc51540tcttatttaag aaccctttgc atgattcct ggaagaactg tttttctctt agaaatataa51600ctaaatttgt gggaatgtgt ctgatatcta aagcttgatg aggattctga ataatctgta51660ttttagataa ggccagttga aactattctt aactactcta tatggttaac tgttttttca51720gagacatgtt tcatcaaagc actaaaatta tgcagacaga gctggaaatg ttctctgaag51780agcatcctat catcttcctt ataattttgg tttttattta acacttagcc cccaaagtgg51840ggccatggca atatctttga gttataactc acatcaggcc ttcgaggagt ttgtgatgct51900attttgttgc tggcatacaa aatcagtgag gttaactgta cttctatagg tcactcagtg51960agtcaacgac tgagctatga ttaacgttga cagctggtga gctgctaggc tgttgctaac52020
tgagcaacat tgacattcat tttgatttga ctttgcattt cattttcaac tatctattca52080ttttaaggcc tctttaaaaa ctttttaggg atgttgtaga cggaggaaac tttctataaa52140tgctttgggg agagtccttt agcttccctc tccccattcc ccatccttca gcttaatagt52200taaattatgt ttatgctagg aaagcatctt ctaggtagtt ttagaaatag atgatttaaa52260tagaagttag gcgattccaa ttttgcttta tttgttttgt aatttgtgat ttcaacaaag52320aatttctgta aactaattgt acaatagtga gtaccaacta gaaaaatcat atttatattt52380gatgaaataa ttcacagaga aatgatgagt atgcattata aatttggcca aaatgtattt52440ttgaacatag gttaaagtgt agctgaaggc catctagatt ataaagcttg gaaccggctg52500gttggctcag ttgggcatca ttttattcac ctgagaggga tggggaaaga gtgagccctc52560tttaacttct ggatctactt ccagatgctt tagggagttt attaagaaaa agtgctggct52620gggtgcattg gctcatgcct gtaatcccag cactttagga tgccaaggcg ggcagatcac52680ctgcggtcag gagcttgagg ccagcttgac caatgtggca aaaccccacc tctactaaaa52740atacaaaaat tagccaggtg tggtggcggg gggcgcctgt aatcccagct actggggagg52800ctaaggcagg agaatcgcac aaacccagga ggtggaagtt gtgctttgga gctaaggtca52860tgccactgca ctccagcctg ggcaacatgg tgagactctg tctcaaattt aaaaaaaaaa52920agtgctattt catagaattc tgtgtgacaa cttaactgcc caatgtggtt ggctttaggt52980gaggcagatg ccctaggact tctgatctca ctaactgcca tcaacagttc tgcactgctg53040atgccagaaa ggcaggcaga tctagaggcc caagtgacct atacatatag gcaaagatga53100cctagcagag ctgaggacat gagatttgga gtcaaacctg ggatcaaatt ttgattcttc53160gaattacttg ctcaagggat aactaaagat ttcctcactg gtcaaaagat tcctcctttc53220tatggaaagt gtgagtgaca cctaaggagc taatgttagg ggtgtgagct ggcagcttct53280aggcaactaa tggttctatt tcttcttcct tctctatcta ccccaacctc agacttactc53340tccaggttct ctgactttca gtgagccatc agagttgaaa agcaagcata tattctgttt53400taaaaatatt tgaaaaccta tctcccaaat ctcagctcat atagtcaatt acgttgttaa53460cttatttgaa gtacatatat tcagacagag ccaaggggtt ttcctcaatt tttagctata53520aaaagtttga taatttaagt gtttggatat tttctaattg ctattaaaag aacttctgtg53580tgatttctca aaggaatcac agtcacttaa aatctggaca gtaccttcaa ggggaactag53640accttcattc tcagctaccc cagaaggatg ggctcctttt tttttctttt gtgtggaccc53700cagtggagat gacagtgtaa ccattggcaa ctctatctac agagtgatca tgaatccctc53760accgaatatg tttacactga gaaatgttgc ttactctcat gtaagtgcag gaaaaaaaat53820gtcagttttt agaatcacaa agtgattcag gaagtgaata aaggccttta aaacatctta53880agattcactg tgaggagatg cttgttggat tttcctttta ggaatatata aatatggtta53940gtctttttta gtgacaaaaa gcaggaagca gtttccctta aatcttacga ttccatcgat54000cgactcatat attatgggag agagtgacgg ttgcagtctg aggtgatttg catgagaccc54060tactttatac tccaattatt actgtacatg attacaggac aacctggttg ttaattgcct54120gtggtcttta aatatgcact tctttcagaa atagacacta attattagat cttgtttttg54180gtctcacccg aagttgtaaa tgtctaggat tttttttttt ttaatttgca agtctaatga54240tatacagaca tagtaaaact gatctcattc tgaatcctgt tcagggtgaa cgttctctgg54300gatgtaaact atcaaagatt gtgaacttgg ctttcagttc tttgtaggtg ttcatgtgag54360tctcttgaca aaacctgtat gtttgctatc agaatgctga aggaatttta tctttctaaa54420atatagatga aaattggtaa acaagttgaa aaaaatctac ttctcccata attaaatata54480atagaaataa catcaaagta ttattacata ttagatattc tcccaattcc accaagataa54540aaattttgtg tttgggagct gtggttggaa ttatgctagg aaaaaggcag tttgttttgt54600gtgttgggct ctcctctttt ctggcttcaa tggctgaatt agggaattga tcctgaattt54660gatagagctg cagttgttcc tgtctttaag ccaaggttgg tgaactcatg tgtctacagg54720ggatagagtg atgaggtaca agtgtgaagc cagtgagcag gaatcctgga ctaacaagag54780tgcacaagcc ttgcctaagg atggcagctg ctactcagtt ttagcagatt tatctcagat54840agaaattctg atttagatgt gaaatatagc aatttaaaaa tattggatgt atgatttggt54900ttttaagaaa gtagtgtgct aagcagagaa aacattctag tgcgacttct agttttaaca54960catgctcttc ccacttcctt tcctggtttt tgtcacccag ctaatgccta ttactttttc55020tctacttgat ttagctgcat gttccaagaa gccttcttgg gtctgtataa gaggtgcctc55080ctgtggctct tgtagcctct tttctatgcc tatatccact tttaaacaat gttataattg55140cttctttatc tttttgtttt gttccctggg ctatagaatc cttgaaggcc tccacaaaat55200actgattgga tgaataagtt tgatgaataa atgatggtgg gcccagggtt tcctcaggac55260atagagtttt ttccttcctt cttccttcct tccttccttc cttccttcct tccttcctcc55320ctccctccct ccctccctcc ctcgctccct tctcttcgtc ccttccctcc tctcccctcc55380gctccccttc cttcctcccc tccctcttcc ctctttcctg tttcctcccc tcccctccct55440tccttcttcc ctcccttcct tccttccttc ccctcccctc ccctccttcc ttcactaccc55500tccttccttc tctcccctcc cctcctttct tccctcccac taccccctct ccctgacgca55560gggtttcggt ctcccagtta atatagggcc tatggccatt ccaaggcttc tcctagaacc55620tccagatcaa tccttcaata aaatctgtat cccaaattcc tgtgtaatca aactgcctta55680gaaaggaggg tgaacaaagg gagggcaggt ctaggaagat ttggaaccca aagccctctt55740gtcttaaagt gtgttgttac ctgttttatt cttgctgctt ttctcctttt tatttcccct55800caatcaacat attctaggaa aggtagagtg cagatgtgtt tttgtgactc aaaaccatat55860gaagccaaaa tgagtctttt tcctgtcata attataagta gggccttagg ctgtgtttac55920tgcctctaaa ataaacgtgg tacttagctt ctcagttgca acacttccca gcttttatgg55980aaaaaaaaaa aaaacaaaaa taaaatgtta ttttaaaaag tagtgtcaga aagtaaataa56040attaccctta atagtgctgt atgttttaaa aaaatatttt ggagggactg ttcagtaaaa56100ccttcaaaac aaaactatac caaacaaact actttctaga tttggcagct ggtcatatct56160cactctatta taactagtca gtctactttt taaatttttt tctcaaatta tgtgtggtgt56220cagagtggta tagtagtgta acacctagtc tctgttgcca ggctaagttc agttttcagc56280tctaccatga acccgctgtg tacttaggga ccaattgctt aacttctgta tgtctcagat56340agttctgtaa aatgaagatc ttagtagtac ctaactcata aagttattgt gaggattaaa56400tgagtagaaa aaaagcaaaa cccttaaaat aatgtctggc acataaatgc tctttacata56460attcacttat tttttgttaa gtcaagtgta ttgaggtata atttgtagta taaagtgcag56520taaaatttac cctttataat ttacaattct atgagttttg ataagtttat aaaatttacc56580ctttataatt tacaattcta tgagttttga tacagttatg taatgagcac tgtgatcaag56640ctgtagaaca ttcctatcag cctcaccatt ctttcatgcc ctttgtagtc agtccttgta56700cccccattcc cagaccctgg caaccactga tctgttttca gattctgtgc tttgcttgta56760cagaacatga tataaacaga agcactggaa tcatgtagta tgtagacttc tgaatctggc56820ttcttttgct tagtacaggg attagtagac ttttctgtaa agaggcagag agtaaatgtt56880taaggcttgc agtccatata gtctctgatg caactattca gctctgctat tatagtcatg56940gacaacacat aaatgattgg atgtggctat cttccagtga aactttgttt acaacaacag57000ggagcagcca gatttgggct gaagaccata atttgctgac ccctgattta gcatattgca57060tttgaagttc attcgtgttg ctgtgtgtag cagtagcttg ttctctttta tttccgttta57120ttattccatt gcttacacag tttgtttagt cattcaccag gtaaaggatg ttttagtttt57180tttttccagt tgaggacgat tatgaataaa gccattgtaa atttttgcct acaggcttct57240gtatgaacac agattttcat ttcttttggg taactatcca ggagagggat tgcttggttg57300tgtagtgtgt gtttaccttt ataagacact cctggccagg cacggtggct cacacctgta57360atcccagcac tttgggagcc cgaggctggt ggatcacgag ggcaggagtt caagaccagc57420
ctggccaaga tagtgaaacc tgtcactact aaaactataa aaattagcca ggcgtggtgg57480caggcacctg taatcccagc tactcaggag gctgaggcag gagaatcgct tgaacccggg57540tgacagaggt tggagtgagc cgagattgtg ccactgcact ccagcctgag tgacagagtg57600agactccatc taaaaaaaaa aaaaaagaca ctcccaaact gttttccaaa atagctgtat57660aattttgcat ttctgtaagc agtgtaagag agtttcaatt gtgttgcatt ctcaccacca57720cttggtattc cctccctacc tttttttttt tttttttttt tttttgagac agaatatcct57780ctgtcaccta ggctggagtg cagtggtgtg atctcggctc actgcagcct ccttctcctg57840gattcaagag attctcctat ctcagcctcc tgagtagctg gaactatagg cgtgtgccac57900catgcccggc taatttttgt atttttagta gagatgggtt ttgacacatt ggccaggctg57960atcttgaact cctgacctca ggtgatcctc ctgctttggt ctcccaaagt gctgggagtg58020agccactgca cctggccttt tttttttttt ttcttttttt taaattttaa ccattgtaaa58080agaccatgaa gattcttgga aacctgaata aagctatatt cagttggaag ctcttgaagg58140gttttataca tggaaatgac attatttggg tttttaaatg atcactccgg ttgtaatata58200gaaaatgata tggaaggagg ctgggtaaat gtgtgaaggc ctttgggaga atatcatgtt58260agtatagggg agagggaaga taccttgatt tgagaggtag cagtggagat gaagtgacta58320gatatggaaa tgttttagaa gatggaactg ataaactcag tgattgagca gatataggac58380ttaagagacg gggagctctc aaggttgttt ccacatttct ggcaccagca actacattta58440taatgacacc cttactgaga tgaggaacat tgacagagaa aaaggtttga gaagataaag58500taattgggtc aattttgaga tatatgtaag acatccaaat ggaggtctca agtaggcagt58560gtgagatctg ggttcctgaa tggtggaaca aaattagaaa ttattgtggt ataaaggata58620tttgatgata tggggtacat aagattttct ctctctctct tactgtctgt tgagtttagt58680gatttgagga gagtgaattg gatagaccta agaggaaatt tggtgtttaa ttttgggcag58740tagagtgact cacaaaggaa gctaagagag agtggttcaa agaggctgga gagagagaga58800gttgtccccc agatgcccag gtgagtgaac aatcatttgt gcatttattg gatttggtgg58860catagttggc atttagtcag ttttgtggtt gttggtagtg gttggatgta tgtgaaaatt58920caaattaggg tgagtgtgag gagtaaggag tgaggcagta gaaaaaaaac aagatttaca58980ggtctatcgg gaatttttag gatagttgtt agagatacat gtaagaggtg tttattattg59040ttgagactga gataggttag agcttggtga tgagaaggac aagggaggga gtgaatatag59100gagaaactgt tgttaatgga atgagatcct cacacactag tgatgccttt cagatccagc59160aaacttgatg aagggaaaac tcagtcaaga ttaatgttca cagttagagt agaatagagg59220acttttagta ttaccaagtt ctaatgaact atatcataat atggtcttta aacttatttc59280tcaagtattt ctcgaaaact taccaagcag aaaatgtcag caatctttct gatgtgtgaa59340aatgctctgg cggtttagtc acttgtcagt ctcatgtcca agtcctaccc atctgtcctg59400gcgcaattga aaccctcaaa tgaaagactc tccctccctc atctgaatgg ccatagcctg59460gtgttttttt ccctctaggg tggcatttat tctttctatg tacttgttat atatatgtat59520gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtataatttt tttcctcatt agatggaagg59580caaagaccat aattgaaata tgttaatgtt ctctgtagta cctactggtt acatttattt59640agttaacaaa ctaaatgtat ataaaggtaa aaaataggga catggttatt agcagataat59700taaaagaagc tatatgaaaa ataagatttt tgtggtttgg tgtcagaatt tgtagcctga59760taataagttt ttgtttttat gaaatttagt aaaacatgtt ttgaaagttc ctaatcatat59820gtgactaaaa gatcaaatta tttaacagag tgataacagc aacaactctg gtggttactt59880tgtcatttac cagtgacttt cagattatta ttccattcag actgaggata atccaaagat59940acaaggagaa atcacaggga tttttgtcac cattttacaa gtgaagaaac cagagataat60000gagtcaacag ttacattgct ggtaaccagg tgctaaatcc caggctggga tcttctgatc60060ctaatgtggg tttctttcag tttacccaat ggcttctaga tttactttca tagaaatcca60120attggcctga taataattaa atgcaactgc agggggtaag tgggtttcct ttatttgaat60180gaatttaaaa aatcacttat tttgggtgat taggataatc tcttggtaac gcctgagatt60240acctcctctt gcacatgctt tggcctgttg gcctagagct tcaaatgggg ctcctgcagc60300agaatgggaa aaacaccacc atcatggatt gcaatgatgc tgtgatttga agaaaagaaa60360atggaagtca catatctgtg gaatactaat agagcaatgc tggccagtac tgtttgctat60420agtgaacaaa cctagtatta gcattttgga aaaaaaccaa acaccatttt cctgggtttc60480tgactctgcc ttctggcaac tacttatttt taaagtccag gatcattttt tatttaggat60540gaaaagctac attctttaag aaactagaaa tatggaaggt cattattttt taggaggact60600atgtcagggc ctttaattat attattgttg aagtttcata aaaactgtaa taatgggctg60660ggtgcagtgg ctcatgcctg taatctcagc agtttggaag gccgaggcag atggattgct60720tgattgattc taggagttcg agaccacgct gggcaacatg gtgaaacccc atctcttcaa60780aaaatacgaa aatcagccag gcttggtggc gcacacctgt agtcccagct actctgtagg60840ctgaggtggc agattcacct gaacgtgggg aggatgaggt tgcagtaagc tgtgatagtg60900ccaccgcacc ccaggctggg caacagaatg aagccccata tcaaaaaaat ccaacaaact60960gtaatggtat atttatatta ctttcaatgt atagaatcta tggttttata ttaaaatatt61020tagtatggaa gtctttgact aaatttttct gaaaaatata attctcatta aacatagctc61080attatttagt accttctgtt aaataaattc tttttaattg tactgacttt aacttatgta61140cccccaaact ctagaatata gttttgtcag gagaactgag gaatgaagaa tgtatacttc61200tgagaaagtg tggaaagaca tgaagtcctt tttcctcctc cttcaccact ttaatatgtc61260atctgaatga attgctagta ctgggtaaaa aaatctcctt atgttccctt ataaattgcc61320ttagataagg tatttgggaa ttgctataca gtgttgtgaa taattgaact tggctgtata61380tgaagcagtt gagacattga atctgaaacc aggtgtccta tggctaaata gttgtgattc61440atgaaaagaa atacaccgag aaatgtaagg attgttttcc cccaaggcag ttggtcctat61500aactctatgt ggactgtgga ctctctttag ctcttatatt ccttatttct attagaaatt61560tccttttagt atattgtaaa atttgttaaa ttgacattcc aaatggacaa ctgaaagtga61620tctgctttta catcagatga acctggccta tgttaaagca gcagactgca tttcagaacc61680agttaacagg gagcctcctt ccagagaagc tcagctactg actttgcaaa acacatctga61740atttgatatc cttgttattg gaggaggagc aacaggaagt ggctgtgcgc tagatgctgt61800caccagaggt aagtcttttt tttttttatt ttaattttaa gttctggggt acatgtgcag61860gatgtgcagg tttgttacat aggtaagtgt gtgccatggt ggtttgctgc acctatcaac61920ccatcaccta ggtacaagct cagcatgcat tagctattta tcctgatgct ctccctcccc61980ctgccccacc ccaaaggccc cagtgtgtgt tgttcccctc catgtgtcca tgtgttccca62040ttgttcagct cagaggtaag tcttgatgtg gataacactt catcctttct cccagtaaat62100acacaatatg cagtctatag tagtgaggac acatttctct cttgtttcca gattttccat62160ccttaatgaa gcaccttcac agtttttgaa atataggcac tgcacaactt taaaatgaaa62220tataactcat ttcacttcaa tgaatttgtt taaaaagaaa actatatttt atgagtttat62280attttatgag tttattaatt tgttctggtt ttttttaaag cacatattta tatttatata62340aaataaaaac atttttatat cacttgaaat cacatatcag tcaatggtag aaaaatttat62400taagctgaag taatttatta aaataattat ctttgttcat gtatctctta aaatcatctc62460ttcagtgaaa tatggactgc agtttgcgaa actttagttc atgcgaataa ataatggaaa62520atatcactaa tctatagttc tgtctcagtc attctgtcta ttagtggaaa ggcatgatga62580aaatggaaac gagacatgta atgaaagaaa ttttagtata gaacaccaat gctcttggat62640atgttgtgtt taataaaata ctatgtttgt tttggctgtg ggtattagcg tggaatttgg62700agtcagggaa atctgggtac ttatcctggc ttaacttctt taacagatgt gtatgtgttg62760gtggacgggg ggcaaagcac ttaatttgtc taaggttcct tgtctttcaa atgaggaaaa62820
aataccttct ctgaatgatt gttgtgagag ttacatgtta atcatgaaga gacttagaac62880ggtgcttgtc acacaaagta gcagctcagt aaattataga tagtattata gctactatta62940ctaacatgaa tgtaaaagac tttcagaacc aattgtcttg gagaagact ccactgtggt63000atcctcagat gtctcgaatt gggtaggata ctgctgcaag gaattttgac tgtggaatat63060tttggttgct atataaagtt caatatttat tatattttaa cactgaatgg gaatgattta63120ttagatggaa taattcttct cagatcaatt aaattactac tgcagccact cttgatttag63180ttactttatg tacctggcag aatctactct tatggaaatg aaatgaattt aatgaggtct63240acacacacac acacaaaagt accctagatg actgatttca caggatccac actcaggttt63300tggtccatgc agacctcaca gcctaagttg tttttgtcaa tgctaaataa tggcaagttc63360aaaatacagg cacaggaagt ctactctgtt acaaagttga ccccaatttt cataaaattt63420tctgtctcca accatgaaat ataatttgag ccagattaac cttgatacac taagcctaa63480aggaagaaca gtttgagaat agtttttgag aatagataat ttgaattcag cttttttccc63540cttgaaggcc tgggaaagaa ggtggaaaga ggggacacat tcccaacata atgggaggtc63600ttgtttggaa ctacccctcc aagaaattag aatttactag gtgataattt tgtatatcaa63660agaggatcat ttccccagta ggattcagga aagtgtattt cttggtatct gcaggcattt63720tcatgctatg cactaaaata tgtagccatt ctttttattt tagttttaca aatatatgct63780gagtgcctgc tgtctgcatg gcgatatgca aggtgctaca atgagcatac aaagatgata63840gagttggtac cgttagtgtg cccacagttc tatagtgctt gtttgcacag accacattac63900atgatagaga atatgtgagc cttttaaaag gctcagggcc attacggaca ctgaaacatg63960agggtagtca tatctacata gaatggtcag gagtgggaaa ctttacttat gctgtttggc64020ataggtaaca ttttaatagg caaacattgc ttgctcaaag tgttccagtc tgagaaaatg64080tgcaggagga acagcgtgga atcagtgtat gtgggagaca gcatgtgttg taatttggag64140cattgaggct atatatgaga gaacacatga gagaagagtg gaaaggcagg gctgggtctg64200gtatgctagg ggaaagcctt tgggctttat tgaatagatc acttggagat actgccttag64260gtttggtgat ggggaatgga tacacgatac tgaaggaaca gtcataagca taggctttca64320agagatgtgg agaaaaagaa aatagaaagg aggtaacttg atggtgtggt aggatataaa64380gaagggagac agtatgtttc acggattttt ttcctttttt ctttttataa agataagagt64440aatctgaata tttctgtaga ctcaggggag aaaagtcagt gctaggggag aagaacctta64500atatgcaagt attaggttgt cattactttt aaatgtcatg taccaaccta atagtagaag64560gcaaacataa ggaacaaggt cttgtgaaaa tacaaggaaa gagatatcag agatgtatct64620tggttttagc aggaaggtaa gagacttgtt tctcagggat agaaacaaag ggtaaattct64680aagacataaa gagtttgtgt gagtatgaag agctactctt aaacaaatac gaggtgagat64740gttctactta tgggtgggag taagggatgt cattgcagag aatgtgacag gaaaataggg64800gtttctttgc tgttagaaaa ttttaattta ttatccacat gagacattta tgtgtgtata64860tatgtgtgtg tttgtgtgtc cagtattact gagagtagag gtacagaaaa tagaaagtca64920catagaatct ggagttgaga actgagtgga tatcaatgat gaaaagaaaa aatgagacaa64980gattctatga aaacagagga aattgttgaa atgtaggcct ggagctgata aggggtcatg65040aagttacaaa gccaggcttc cctggaagag ttaaaggtca gattgcaaga cttcaagtgc65100agtaataata aggcaatcac taagtgactt ttattctttt tcaccttctg aaaagtctta65160ctatatttgc ctttgtgcac tttgtatatt tatagttttt aaaaaaatag acaagattgt65220atttcttttt tgcagtgtgt ttctttcaaa tagtagtgtg tttatatcaa tctttacatt65280tatatagtat tcatgatttt taatgggaaa attccatttt atttattttc atttaataac65340tttatggaaa aactcattgc aatgatgttc ttattaatta ttctttaggt tgtttttggg65400tggttttttt tttttctatt taaaacaatg ctatttgtca atcaagttct gaaccagctc65460tgagaatagt aagactagta agatgtggcc ttgatttcaa ggaacaactt agcagtttat65520tactatttat tgaacaaata aactttttct tctggcagtg aagggcattc tgtagtgatc65580aaggtattag ctagtatatt agcagatgat tgtgggtgat gatagttgtg gtggtgatga65640taaggatgac aattttgaat agttaagagt ttccagagcc tgaaagttga tcaggttttt65700aatttttaca gttaacagta gctaagaaaa cgatgtattt ttaatatatc ttggcatatt65760aattgtggtc ttaaaataat tgcctgtctt attttatata tgtggaatta tgatttataa65820aaaggatggg caaactttag cttgtatttc tcttattatg ctggtagccc tctgggagta65880gcatttataa ttaaaattta taaagagatg gctgaaggta atctatattt atccttttac65940tacaattcct gatcatatca gtggccctct gaagtgtgaa ataatcatgg gcatttggtt66000aatgaattgt gcattgttgc tgttcattca tggaatttgc tcttttatca tatttgatgc66060ttcacttaac aacaaatttt gctttctctg catttgatat gtttttacca aattattcct66120gattgtatcc attaatcttt agtcacattt gacaaaaata aattactgtg atcttttaaa66180gttaccagta ataattagat ttttcaggac atcaaagttt tggggggaac cactagatgt66240taaaggactt gtacaaaaat gtttgcatga gctttttgat aaaatactgt gcccaaatgc66300aattttagta atggataaac aaagactata gaggacagac aaaaagtcat ggagtcttca66360taatgaacat ttatttgcat tcttatcatc tgcattttct tgggaatcaa ttttcatttg66420tttcatttgg gaatattatt ttataaacac aatatgcaga cttctcctca cgttctcctg66480caggtctcta ggatcaatag aaattgcttc actctttaac acctctgtgt cccgttctag66540gacagaagca tgtcccaaaa gcccagttgt gggcactttg ctcccaaagg aagctggaaa66600aggagaatct gttaaatata ttcactccca caggttcttc aaggtgttct ctttagcttt66660ttatgttctt ttatgtaact ttacctgtat gtatctggga ttatctagta catttttggt66720ttaaaatctg atgtatttgc attcttggtg ggcctgtaga aaataaaata agatctgatg66780tataaaattt aacctttatg tcttcagttc aaattccaga gaagactgcc tctttctgga66840ggaaaatatt aattgtagtt ctcttgttta caaatggaga cttttataaa tggtatttgg66900ctagcaattc ttgaattccc actataattc ctaaggcagt atagcgcagt gattaaggac66960acatattatg gaattagaat gaacttgggt catgtcttgg cttcattgct tactacctct67020gtgaccttcg acaagtggct taaccttaca gagagcctta agtttctcag gcataaatgg67080aggataataa taacattctt ctctataaat aaacatttgg gaaaggaagg agatgtgtgc67140attgtaggat aaatgcagaa aagggagagc cttggagata gtgcattctg agaattatgg67200tctccaccca tccaccatga aggtaggaga aaattctact tcagaaggta gaattgcagg67260aagtaaaagt ttgctctttg ctggacacag ctaattagac tagattgcaa atttctctta67320aaattaatct ggagatttaa cataatttta attaatgtag tgctttcctt tggccagggt67380cagcggaatc gacttaagaa actgtaaaac tttaagaaga gaactacgcc tgtgagattt67440actagactga ttttgaaaaa gactactgat aagggaagta gagacctatc agatactaaa67500gtgtattcta aaactactgt aattaaaata atctggtaaa ggaacagaga atcagtcatg67560gagtctataa attagtctat cagtctttaa atacatgaag tttagaatat ggtaaaggca67620gcatttcaaa actgtcatca ttagttactt aataaatgat gttggggtat ccactccccc67680cgatttatat atatgcatgt gtgtgtgtgt gtgtgtgtct gtgtatgtat gcatgcatat67740atattctgtt ttatcatatt ccatgtgaat caaggatgta actttaaaac atcataaaac67800tattagaagc aggtatatgt aaattttttt taaaaattgg gtaggaaagg tgtttttaag67860tatgacagca aaaagccata aaggaaaagc ctgcttttga ttttataaaa attaaaatgt67920atccatggca aaagatgtaa aagcaaaaag gtgaaatttg ggaaatattt acaaaatagc67980aaacagacag ggtttatctc aggtttttat ttcaagtttt gttttgtttt gtttttggag68040acagagtctc actctattac ccaggctgga gtgcagtggc tccatcatag ctcactgcag68100actcaacctc ctgggttcaa gagaccctac cacctcagcc tcctgagtag ctggatctaa68160aagtgtgtgc caccaggcct gatgagtttt taaatttttt gtagagatga ggtcttgctg68220
cattgccagg ctggtctaga actcctgaga tcaagtggtt ctcccacctt ggcctcccaa68280agtgttggga ttacaggcag gagcctctgt gcctggccat tctgcacttt tctaaatcct68340tttgttatgt tatatctgtg ataaatagta tatagctaga ttatatttat ttgttttatt68400tctttttaca cttaatagac tatgtctttt aatcagtaaa tggaatccat ttacattttt68460tttattccat gtgcatttgc tttgtatttt ttttaatctt tcatgctttt tattttgctt68520cttttccccc ttttctgccc ttattagatc agttttgttt catgctcttt ttccataccc68580tcaactctgt aagtatagta atgaccatga gagacttttc ttggaaccaa aagactcctg68640cccttgttga ttttggggac cttgcagact agctgctgag acaagtggtg ccttggctgt68700ggtgctggtg ccaaggctgt gtctgtcttt ctgcttcctg ggcccagaga gtggtcaaga68760gaagcttgtc aacctccttg cacaagaggc agagactttc ttcagtccca gcaatcaaat68820gctcctctgg cttttttacc tttccttgta atggatattt atattcctct tacccttttg68880gaactgagat cttgattgcc tttgcccatt tttagacctt gaacctagca agagagaagc68940ttttggtcta aacattctgc tctgagtttc tgttacattt caagtccatt gagttttaat69000cttatttttg agtcaattat gtcctttaaa atatatgcat atataatttt ctgtgttttt69060aagaagaaga agagttcaaa gctgagctta caatgtcata cgggtcagaa ttttatttat69120aacaatattt ataatggcaa aaaattggag tctggcttaa taaattggat attcagttat69180tgaaaagaga tagatgtata agtatttaca gggaaaatgg ctaagatgtg ttacaaattt69240taaaaaagca agttgtgaag aatgagtgct aatttttatt tgtttttaaa gcgagatggt69300atcagatagt gttctttagt aagcataaaa aagtttctga taaaatatat aagacagttt69360taatagttaa cattgtttac ctgtggattt tgtaactagg aaaccaggag gccattagta69420tagtttttta catcattatc tttttttgcc tttctatatt atttggatat tcttcctctc69480tttgaacatt gggtatgttt gataatacca aagtaaaaat aaaacgcaac agcagcaact69540cccttattcc tcaaaattgt gttattgcaa ttgttacagc ctagtacagt tttgtgactc69600cataatcatg tgtgtactta acaaacatta aagtatgtta atgatgtgtt ctaacaacat69660catatctcta ttaatctgtt ttctaatata tctgttttct aatataaaca tagaaataaa69720taaccaactc aatatcatat atttatataa aatgctaata ttcacttttg gaaaatacca69780ggccaaagca ttgtatcgtg atcgtgagga ttaacaagtc aggattactt tgcagcttta69840caagaggata atattttaga ggaattgatc cttcccataa aatgttcagc cttcgggtta69900gtcccttttc ttattaatgg caatggctgt ctgaaatcca aagttaagtg cccaaagctt69960ttctaaagaa agataaactc acctttaaaa aaaaaaacta tattattttt cctacatgca70020gcatgtggct ttaatacctg ttcttatggt gctaacatac aaagcatctt aaattttttg70080attttttgtg ctattatctt ggtgtctctg atacttattt cagagtgatt attctcacct70140ttctatttag aaatatctta atagtgacat ttcttaaaat ttgttctaat gatataatta70200aagatacaag gagtttaaat gaaaatataa tcttgcagca ctatctgtaa tagtgaaaca70260atgggataat ccaaatattc aaaaataaga aattgaataa attattaaaa ttaatagaat70320attatccaca tgttaaaaat gaaattatag aaaaatattt attgacatgg gtaagtgttt70380acaataaaca gtgttaaatg aaaaaaatta aatcaaatat accatatgat cctattttta70440caaaaatgta catatgtgat tatatacatg catagaaaat gtgaacgttt aaatacccaa70500atgtcaactg tggttatctc tgagtggtgt gattacagga gagttttatt ttattctctt70560tgcttatctg catttattat attgagcatg ttttcatgca taataagata atcagtaaaa70620ggtattaaaa tatacttctg ttctatttca tgttgtgaaa gttaaatgtc gtaccatttt70680tcctcttaga ttgtaattat ccttctatat tcatccaact gttttgatcc atgaattggt70740ttagttggaa gtaattagaa attctcactc aggtcagaaa agacaaagct gtaaagagct70800gtgttagttc ataactcttg ccccacctag aagatacaat ttcagtttgc taacagatga70860gatgataaat ggaatataga tttcaagttg actttattct tctactctaa ctaatcttgt70920tattctgcct aagatttcag gtaatgttac tccaaatatt agtattttat tactgtcgca70980aaattcccca gactacctac taaaagtaat caataataaa aacaaagcct taaataatga71040tactaccact cccactaaat gttctgtttc atcttaatag agatacagct tattgctttc71100cataaaaagt agctgctaac aagaaaaaag aaaagaaaaa acaaacttac tataaaatct71160tgaactaatt tagtgaatta ccttcactac atagagaggc cagcccttcc ctgcctgtaa71220tgtaaggagg tcactctttc tactttgagg gaagatctct ccccagcctc ctcaaatcca71280tagtaaggag tagtttcttg aatcctgggt gcattatttt ttatgccctt caggcttaat71340tattttacct aagaacacaa aatgctagca ttgaatatca cttaatacta ttcactatct71400gagaagataa aacacctccc tcttttcact tccttgcttt atacttaaca taccagaaaa71460ctgaactctt aatttgattt cagtaatttt tgaggtgagg aaacaatttg gatttttgag71520aattgaaaca gaaagggaca gattagataa cagaaccaca gaagaacctc aaaaggcttt71580taaaaatgtt attgtcttca ttgcagttgg ttggttggtt aattagttaa gaaatagttg71640agttgcagct atgacttcta ataatgtctt gattttattt tcctcctttc tacttctcat71700tcaacaaaaa tgagagattt aaaacattta gcatataata gcagttgaaa attaaagaaa71760atttttagag ttttttgtgg tttggaaaac tttcagtaat acttttatgt tacatggcat71820tttaatatat caatttagct ataactttgg aaactaaaat tttaagtttt tgctctggaa71880ggagcagtat cgtttctttc cagaaattct ggtgggtaac ttgataattt gacactagaa71940ataatgggac aagaaaaaca gcctggtttg acagagaggc ccattttaac ttttgttggt72000attcactagg gtttctcaca taagtacatg tgataacttt taaaatgtcc aatactggaa72060aaagcagttt tgagagcttt aaagtgttag ctctcactta aatatcctga aatatcctcc72120tgagatggct tgctaattcc tagagtagtt ccctgcctca cactctgtca atttgggaaa72180ttaaaaaaaa aaaaaatcca tcctgacaac acagcccata aaattttctc tttatatcac72240ccttcaatac catctctgcc ttctgaggcc ggattcttca ttgccccaac tcctgttata72300tgacactggt cctccaaaag gtctcctgcc tccagtccta cctctacccc tcattcctgc72360agtcagatta atcttttctg aaccttaatc tgatttctca gctgaaaaca ttttgatagt72420tctcctgaca gtcagcataa tagtatttct caaaacaata tctgaggact tctagaaaaa72480atttaaatgt tattcctttt ttataataat aaattaacaa gcacaccctg gttccccaga72540atggatagct tgtaactgtg taaaatacta aaaatactaa tattagtgct tgcctgtgca72600tgctacatct tttttttttt tcttgagacg gagtctcact gtatcaccta ggctggagtg72660caatggcacg atctcggctc actgcaacct ccgcctcctg ggttcaagcg attctcctgc72720ctcagcctcc tgagtagctg ggattacagg catgcaccac cgtgcctgac aaattttttt72780tttaagtaga gatggggttt tgccaggttg accaggctgg tcttgaactc ctgacctcaa72840gtgatcctcc cacccctccc aaagtgctgg gattacagtc atgagccact gtgcccagcc72900actttttctt tttcttttct tttttttttt ttgagatagg gtctcattct gtcattcagg72960ctgaagtgca gcagtgcaat cttggctcac tgcaacttct gtcttttggg ctcaagccga73020ttctcccacc tcagcctccc aagtagctga gaaaacaggc acatgccact atgcccggct73080aatttttgta ttttttggtt gatacaggat tttgtcatga tgtctaggct ggtcttgaac73140tcctgggctc aagtgatcat cctcccgcct tggcttccaa agtgctagga ttatgtgagc73200cactcacacc tggccacatc ttgttttttt ctgatgaaac atttgtttct taaactgatc73260caggacacat agctgtgttc tcagaggcat cttccttttg aggagatcca gatattactt73320acttgtgaga aacagtggtt aacaggacaa gatccaaata cctgaatgtg taagaagagc73380cctctgagat ctgccttgcc cattctttgt acctgcactc caacccctga gtgacaagca73440gcccctggat aagctaggcc acatgaagcg tttgccttct ctttcttaag ctgggctttg73500tcgatgatct ttccctttct tcactccacc tgccctgaac acgtacttct ctgtagactt73560ttctttcctg atgactccat ccccaggtac aaggaaaact cccatctctc ccatcttggc73620
tatagttaat cttcacacag ggaattgtgt cactacaaaa ctatccccaa aacttggtgg73680cgtaaaacaa caattatcat cagccagttt cagtaagtca ggaatctggg tgagccttag73740ctgggtactg tggctctgca tccctcatag tggacagtgc tttctaataa ctcgcatggt73800tgttggactg aggtctctgt tcctcaccac ctgttgacca ggagcctccc tttgttcctt73860aactcacaac atgacagctt gctttaccag agcaagcaag caggagggca atagagaata73920ctggaaagag agagagcact tcccagtctt taatgaccta atcacagtag tggcacatca73980cctgctcatt agaagaaagg tgctagtcta gcccacactc cagggcaggg ggtgacatac74040tatgcagttg ggattactgg agactgtcta agtagtctgt ctaccatagc ctgtaggata74100ccttagggta ggtatcccta aggataggcc caggtgagga agctgtgggc tgtgggggag74160gctgcttgtc ctctctcaac cttgtttccc tgcctctcat ggtgtgggca tcttgatgcc74220atgacagtga ttccagggtt taaagagagg tatattttaa ataatctggc tcttaaatat74280catttgaaag tcaactccct cgtctgatct tcagctgaag aaaaaatggt ctattttagt74340gctagaagaa gaactctgtg ttggacttac taagaaaagg tgatatagct cactgagata74400gtgcattctt aagagatttg cagatttaat ttttctgggt ttatatcctg aatttacctt74460ctaacgtgga gtatgatgag agtcttctgg attttcaaac ttggcctcta ttaaactgct74520gcactttgta tacttgcttt tctttgagcg gggagatacc tgacagccta gttcatgcct74580aattcacttc taatatcagg attttggctc tcaatattct taaaaatatg tactaatatt74640tttctgtgtt cttagaatat tcactactaa attcgctagg cattgctagg gttttaatag74700ctgagaattt tacaggtctt tctctggcat atatacttaa aaggagtgac ctggggctgt74760gatacctgta acttttgtgt tatttttcta ggtagttaag gcattaacag tttttgccct74820tgaatcaaag ttctctactt ttctctccac agcttctaaa tattttgtcc ttttgagtca74880agggaattgc cctgatttta tgatgacatt tgggtacgat atttaaactg ttaaaagtta74940taaaaacgac cctttggtat taaatgtaat aagtggcttt gaattgaata catgcacagg75000ttctcctaaa tgaactttgt gagtccaaac tgctctagtt acgtattctc attgtaactg75060gattctatga cattctttca tcctccaaga gtgttccagg ccatgaaaac tcctcatggc75120ttggtgtcga tgtattcttt gggtcttttg gcgtattggc tttcatctct caactcctgt75180gtggcacttg tagtactgat ggtcatctcc agtcctgaag aggcagggtg tctctgcagc75240aaaacaaaac tagttatccc taggagatct ataacagccc tcaagtaaag ggggcatggg75300caacttctag aactgaccta tctttgctca gcctttcctt atcaagaata tgttcttctt75360tttttttttt tttttttatt tcctgagatg gagtctcacc ctgtcaccca ggctggagtg75420cagtggcatg atcttggctc actgtaacct ctgcctcctg ggttcaagcg attctccggc75480ctcagcctcc cgagtagctg ggattacagg cgcatgccac catgccacca tgcccagcta75540attttttgta tttttagtag agacagagtt tcaccatgtt ggccaggttg gtcttgaact75600cctgacctca agtgatccac ccgcctccgc ctctcaaagt gctgggatta caggcataag75660ccactacgcc tggcaagaat atattcttta ctcatggtta ctctgcacag tgttcatttt75720tactggcttg gatttctttg taattctaaa atattgtcat gttcaaaggt tttaaattag75780actataaaca cctcaatatc agaacttatt ctctttcttt ctttcttcct ttctttttct75840ttctttcctt tctttgctat ctctccccat tgtatatcta ccataatacc actccagatg75900catttctcaa cagaagtcaa cacacagaag gaagtctagt ctagatttat tcatgatgca75960tgcattagag tagatcagag atgaggggct gcgacaggtc tggactgttg tgagagatga76020acgagagttc tagaaggctt tgcaacaaca ttcagttaac tcttcatggg tagagttggg76080tgctagtctc tcctttgacc ttctaagtgc accagagtat gttactgcca tgaaattgca76140ctgtaatatt ttctagttgt gattcattca cagcttaata tgcttctagt tctggcttga76200cttttccccc aggaagagcc taaaatctct tcattataat atagacagtg tactgcactt76260tgaatacaaa tttatccatg ttcagagtaa tttttcttgt gattgtctct accctggaga76320agtgccttta atgaattaca tacaaattgt gtaatttaag aagttaattt ggttttctgg76380ttacacagga ctaaaaacag cccttgtaga aagagatgat ttctcatcag ggaccagcag76440cagaagcact aaattgatcc atggtggtgt gagatatctg cagaaggcca tcatgaagtt76500ggatattgag caggtaattg tgtatgctgg ttgttaaaca aaaattgcaa ctgtgctttt76560ttctacccaa ttaaatcagg tttttttttc tttaatggct taaaagcatt tcttcctgtt76620ggaaatacct atttagtgta ttctgctgga atatctttca ggagaggtgt gtcagttctt76680tcctgtagta ttatgtttat gggtaatcag gggctttttc ttcacaagca tgttgaagta76740gcagatttac aaaatcactt ttgaatgtgt tgccttttaa caattgtgtt tgggtttttg76800ccaatggcta ccaaattgct actttgcaag cactttcagg ggagctatgt tgtaatgcag76860ggggagtgcg aaattgatcc tcttaactac aactgcagtc ataggctcat attgtcactg76920acatattctg aaggaaaaga tgatctgagt catgcttttc ttatgtcaca ctttattgtg76980tccagcctgc caccatttga aaactgccaa ggcagtgtgg aagatgacat ttcccagagg77040ggtaaaaaaa atcagctgaa cttcctatct ctttttgtac atgaaaaaca cagtggcact77100ggcttgacat tgtcaagttt tgaatctgtc tctttttttt tcttgacaag tcagttttga77160atctgtcccc ttatatttct cttcagcatc atgatggagg caaagaaac agcataggat77220ttagaatcag ctaacttgtg atgtgttcag gcttatttac tcaaaatcta tgagtctgtt77280atcttatttg taaaattagt gttataatag caatgctggc tttttagaga ggcgtaatga77340ggactacagc agttaagaca aaagcctttt tataatctgt aaaatactgt gcaactgtta77400agaagcatca ttgcttggtt tcatgaaggc aagatggaaa cttttagcat gaatttgaac77460aatctgttat ctcctacaca gaaaaaatat tatggggaac tgagaaaggg agacaatcca77520gaggatgcaa gataaatggc tacaataatc ttgtaactca agtcaggaaa atatcttaca77580cctgggaact ctgacagcat gtttttctcc tatgcaaata cagttaaggt ttctaaaaaa77640tctttgaggt tgacagcatg atgtattatt agaaatttga ttaggaggga gctatcctca77700ctcagagata gaaggtttct gtgtatcaat cagttggcag ggtgtctatt tgccatttga77760aaatattatg atctgttact gccaaactaa ttttctctgt tttgcctttc agtataggat77820ggtaaaagaa gcccttcatg agcgtgccaa cctgctagaa attgctcccc atttatcagc77880tccattgcct ataatgcttc cagtttacaa gtaagccttt tgatatcacc tgtatgcatt77940tgcttctctg cggtcaatag aacaacagtt ttaatggaaa taatccctca atgcatattt78000cataatgtgg ttttaattgg tcctaatctt taatcctttt gaagtatttt cctttgttgt78060atttaaaagt atggcttttc ttaaattact ctctactggc ctatctctag attttaaagg78120caaaggaggt cgtattctgt atttgtaatt agtagtttct tactccagtt tctgagagta78180tatagatgag caactgcatt tgcattaagg aacataagag gatcatgtgc agactgtaaa78240tggttgagct aaagtatcct gttataaaat gtaaacatac cacataccac ttgaaaccca78300aggcattttg agtcatgtta cgtgtctgag ttaaccaagg ctgaaatttg gttaggaagt78360gtcagattat aaattataaa acttaatttt cctaatgttt ttagagctat ccatgaagtt78420gccttacatt ggaattcagg tagtatgaac aagtagatta tgtaactctt ttagccacat78480tagtgttgta gggggctgga aacagatttt ttataatttt gttcatcctt catcatagat78540cgcttaggac ttcctgaacc agtgattctt aatcctgggt gtacattgtc atcatccagg78600cccccaccct agtctagtta aatcagtttc tggatgtatg aggcttaggc atcagtattt78660ttaatgttcc ccagagaatt ctaatattca gccagtttga gaacagctgc ctcagagaag78720attcatttaa aatctcattg atttatttta cttatagact tcagctaggt tggtgtcaaa78780aacagttgca taagtctgtt tcttttgcag tccacttcta ggtatgcttt gtttttctta78840aatgaaagtg tcttgtagtg taaggtaata ttctataatg ctatactctg tttctgaaga78900actttccccc ctatttatgc tgtaggtggt ggcagttacc ttactactgg gtaggaatca78960agctgtatga tttggttgca ggaagcaatt gcctaaaaag cagttatgtc ctcagcaaat79020
caagagccct tgaacatttc ccaatgctcc agaaggacaa actggtagga gcaattgtct79080actatgacgg tatgtgatgt tttttttttt tttcctcaca agatactttt ctctcactca79140tgacccgtat agtgtattct taaggagata gttttgcatc tttaaatact aatcttacac79200aacacacaaa cacacgtgca cgcacatatt caccattttc tctaacggtg gctgaaaaac79260taaccatacc attttacctt taatactgtt tattttacat atttgttttc ttctttgatt79320tctactttgt gtctcatggt tggtttggtc catttctcat tattatgaat ttggtcccca79380ccctcatgcc atgtaatttg ttatgtgttt gctagtgaat atttgcctta tgaatatatt79440ataatgttga aagcagctga ggatatgtta ggttttctag atgaaacctc cattttcaag79500gggttataaa cctaggggga aaaatgccct gtgggctgaa attgacatgt ttgtattttg79560cacaggacac aattcttgaa tgtataattt atatgacgaa ggtagatgaa cattgcatct79620gttaaactgc tctgagtttt tattgcttat tttattgttg ttagatgttt tctaatagtg79680acattacata gtggccattt gccatgtgtt caatgttcat tctttttttt tttttttgca79740tgtatgcaat atagttttgt acacttttat ttgaaggaac acttaatgta tctccagatg79800gtccacaaaa caacataaaa caaaacaggc aaagccattt gtaaaatttt actagtagtt79860ttaaagagtg tgtgagattg gaatatagtc caagggtgtc catttataag gaaatagctg79920aacacagata attatacatg ttcaaaaagt cctaaaaatt ttgtgatgca gaggacagct79980tagattttat acctcagtga gccccccacc cccgttgtat tatcttttct atctagtatt80040agatatctaa actcttagca agaagaattt gttgttgttt tatttttttc aggatacttt80100gatatttata ctgaatacta tgggatgtcc caaaggctat ccctgtgttt ttttcttctt80160tccagtgtta catttctgaa ccaattcagc tgtcaggctt tcatatataa atgcaaaaag80220tatatgtaca gagagggaaa aggggaccaa actcaagaaa atgtcctagg atcaacaata80280ttagaaaaat gttaagggtc ttaggtaaac attaaaagct agaaaattca cagtttacag80340tgcttccgag catctgaaca ccacaggggg gccacttaac tatttttttg gtgcccttga80400ttgagtctac atgttaaagg acaaccttaa ctgtgaattt gctttctctg cattggaccc80460ttaatgttaa tccattgtag tttattgggt gattatagta aagaggtttt tggtccagga80520ggagtatgaa ggcattctgt aaatagcgtc ctgcccatcc tgacatggaa atgtttgtgg80580cctttccctc ttattactaa tccctcctgc acaaaccctc tcacatactt actcattttc80640tatactttgt gatctattag gcactaacca attttatttg taagcttgtc aatatctgaa80700tcttttaaaa ataagtatta gtgggctggg tatgttggtt cacacctgta atcctagcac80760tttaagaggc caaggcagcc agatcacttg accccagttc aagaccaaca tggcgaatcc80820tcatctctac taaaaataca agaaattagc caggtgcggt ggcatgcact tgtagtccca80880gctacttggg gggctgaggt ggctgcagtg agctgagatc ccgccattac actccagcct80940gggcaacagc atgagaccct atttcaaaaa aaaaaaaaga agaagaagaa aagaaatatt81000agcaaatgtc atatctgaat ttgtgacatc ctgttttaca ttggtttggt attgttgtga81060attatagatt cttatttgga attgttgtga attatagatt cttattgatt ctacatggag81120aagccaaggg aaacctttgt tacacaaggc agaagaaaga ttattctctt agaatcatga81180ggaaaaagaa ttctttaatc tagaattgat ctttatagcc tgttataggt tgcaaaatta81240tgagcttagc aaaaattcct gaaaaattta aaaagtattt aaaatatttt caaattgctc81300tttggaaatt acttgtaaaa tcttaagtct tgaggcaaag gagtaaaaat aagtatattc81360tactcatttg atataaataa tttgtctttt gaccttgttc tgaaatggta caggcccaac81420tatatcatac ttgaggtgag gcaaaacaag catgtgcaac agtgtagttt tgtggtttta81480caactatatt tacatgtggg gcaactcttg ttttacataa tatggtgtga tgatcaaatt81540gaatatcgac atggaaacaa ggtgacgagg agcatgtagg taaaagaatt aaatgagtaa81600atccatgctc aacaaaagga atgaacgcct tttttccctt tgactggaaa aatgttatta81660cagtatcatc ctgctactaa agtatttcag agaggaaaat atttcaatgt gaacttttat81720cattgcaaac ccaagtctaa acatggtagt ttataagata agcttttctt aacaaggaaa81780atacatactc ttcataaatc accatgatga gaatataaca atagtcactg aagtgatttc81840aagttgcatt caggcttctt gaaataatgt tgaatttact ttaaaaataa catattgagg81900tgaaattcaa taaaattcaa ttaaaattag ccattttaaa gtgaacagaa ataattttta81960taactcccac caagggagaa ttttgaatct gatgagaaag tggcgtaagt aatcaaatga82020acttttaatt ttaattaatt aagttttttg agacagagtc ttgccctgtc acccaggctg82080gaatgcagta gcatgatcat agcttcccac agcctctaat ctccggggct caagcaatcc82140tcctgcctca gtctcccaag tagctgggat tacacttagc atgtgccact atacctggct82200agttgttttt tacttttgct agagacaagg tctccctgta ttgcctaggc tagtcttgaa82260ctcctgagct caagggattc tccctccctg gacttccaaa gtgttggaat tataggcgtg82320agccactgtg accagataat caaatgaaca tttaatgctg caagaagttt tgctaactaa82380aaattcttat tagtaatatc ttatacactt acttcagcaa ccatgaaaag tgaaatataa82440tgatgtgaaa atatagtggg acaaactaat aaatgttgcc attaatatgg accaggcact82500gagaagatct gtaaggtatc ttcaacgcag agatgttagg gctgtaagag gtttcctctg82560aagtacaaaa tgtacaaaga taaaaatttt aatttctcat aaacacagtt catcctaatt82620aaattctgga tggctattgc atatgatgaa ggcaaagttt gttctcccac agaagttcat82680agaggtaata aagaagttcc atgcactctt ccctccaaca ttttatatat tttttttaat82740ttttattttt tgtagagaca ggctggtctt gaactcctgg cctcaagtga tcctcccgcc82800ttggcctccc aaagtgttgg gattacaggc atgagccact tcatctggcc cttcatactt82860tttgatgtag taattatagt tactgttctg tgcatgacat aagtatgatc tgtaccatat82920tttattgtta accactgaaa aacaaattca cataaatgga ggaataactg tcatattttt82980aaatgcagac agagagaatc tcaaatttct cagtgaaatc catatgaaat gaaaattggg83040atagtaaatg acataccagt ttttcctcca gttgtatttc cttcttatat tctgttagag83100taatcatata gtcaactcac tggttttctg actgagacat gtcctagcat tttctaactt83160gcctagaaga gacccacagc tggccattac tgctgataag ggagttatca agtagagagt83220atggtgacca ggccaacttt ctcaagtcag aagaaagtgt ttagggcatg gcatgcctgg83280gtgacttgac aggctgtgag attatgtgaa ccagctgtta ggggtagcca gttttctcag83340gaacttattt tttttttcct ctatttgttc ccagagtgga tctttgtggc tattctagtt83400agactccagt catccaatct agtacacaga aaatgagcta taattaaaaa tcattaaata83460ttaaaaacca ttcaatacct ccattaagcc attataaaat gaaactgaag aacaaaatta83520cagctttaaa ttcttccttt ctgagagtca agacattgac agagatgtct gttgtttcat83580acccgacgat gcttctaagt attccttatg cacatatgaa aatagcaact ataagaaatt83640acattgcctt ttcctaatgt aaataaaagt tggttacatc catatttcaa agggggaatt83700aaagcccagg tttcactaga atattcattt ttattccaac ctaaaattca cagcatacaa83760atagatgaat tcagctcgtg agagagcaaa gccaatttaa tctctggctc ccctgaggta83820aatgtagttc acggtcctgc ccccacccca acctcagcta taatccctgg ctcctggagg83880taaaaatgca ctgaagtgag gaaaattctg cagcaggata taatgatcca aaatgacagg83940gacaataatg acaatgaatt tcatgtctcc cagtcacgag ctggaggtgc ttgactaggc84000tgacttatac aattaaattg ttacaggtac agcaggagat gcagttagct gtatgctttg84060gggaacgatg cctctggcaa aaggcatatt gtggaatcca ggctcttttg ttaggagcaa84120gctattactc ctctaggcat gcacctgcaa ttgtacactt taataaaaga aagcccgggt84180taaagacaag tattttaagg tgttgatttt atctacttgg aaagctaaaa aaatcaatta84240ccagtatcac aaaagtgatt tttttggtct ttttgttgtt tgttttttca ttgagttcct84300ggaacataag taaaatctta agatggtgcc tttaatcctc atttacacta tttgtaaaca84360gagttgttga atgaattggg tgttacctac tttaaatcag acagatatta atttcttcaa84420
aataatagaa atagattgaa cagataactg aacaatgtgg atttacaaac agaatattaa84480tagcaagtcc tttaagcaag tttgggctac ttgcttctta atgcaattct tgtgaattgg84540agtggtaatc tccataatct ccaaagtgaa agatgtgagc ctcatgaggt gcacacaccc84600atccactagg gtataggagg aacacattag aacttctact tcgatatgtt tttaaagttt84660aattccttat aaatttgaaa tggttcaaat gtctataata tataaaaaca gtaatatgta84720cattatgtaa tttataaata aatatacaca tattggagat acatactgat atttactttt84780ggaatagtca gtttaatttt tttggggggg ggccactagt aatttgtcat tattttattt84840ctttgataat tggctattta aatattttca agatagatgt gtttagtgag aggacagatt84900gctttggaag agtaaacata accaaatccc tttcttcatc tcccaatgtt taatttctat84960gaaatttaat tgaggtggct gaaaacataa aaacaaagaa ctattgaata agaggaggta85020ttggcatgat acattaaaat tgtgtattta aagaaaattt tcatcttaac ataatgcatt85080tattttaaaa tgtaaagtcc tatttgccaa caatttccag ggctcctacc ctttttgtat85140ccttattatt gtgcaaccaa ggtttaaaat tatgtaaata ttgatacaca taaaagaata85200cgtgtaacac gtatgtaagt tgtaaagtta gtcattaaga ttgtgggatc tgcaactgtc85260cttaaaacct agtgagatag gagagttgcc tggacccttc gtgggacttg cgacaggggt85320gtggctcgat tacctggccg ggtgctcaaa ccccttgtgg gtgggggagc acccaggcaa85380acaggtgcct gggccagggt gagtgccttt gggctccagc cccatggcag catctagggg85440tgtgttatga ttaatgctct tttggcagtt gccatccaag atggctaagt attaagcagc85500tcagtggaga gtcagggtga cagcctttta caccttgcct tcttggtacc caggttcttg85560cccagcatct gggaagaatc aggtcataca gacttggagg atggtgaatg cagaggtttt85620attgagtggt ggaggtggcc ctcagtggga tggggagctg gaaaggggat ggagtgggaa85680gataacctcc ctgtctccaa ccgtccagct gcctcttctt ttctctcatt ctctgctatg85740ccactccact cctctgccgg tggaatttgg ggtttttata ggtacaggat gggggtgttg85800tgggtcaggg tggtttttga aaaaacaaca ttcgggtggg aaaacaggga tatgaagttc85860tcatttaggg ccatgggccc aggcttgtgg atggggccat tgccggggaa ctaccctctg85920ctacccagta tttccctgcc tcctgtccaa tatcactagg atattaccaa gacattgaag85980ccaactgtga ctattcacca tttcatttcc ttgtcttccc tagaaataac tgctatgctg86040aattgtatta ttagggttcc tggtctttct aataatctta ttatatgtac atgtatttct86100aaagtaatgt attgttcagt tttatttatt tatttttaaa attattttta ttgttattta86160tttatttatt tatttattta tttatttatt tattttgaga tggagtctca ctctgtcgct86220caagcagggt ccagtggcac gatcttggct tattgcaacc tccacctccc gggctcaagc86280cattatccca cctcggtctc ctgagcagct gggattacag gcacgtgcca ccatgcccag86340ctaatttttt tgtatttcag tagagatggg gtttcaccat gttgcccagg gtggtttcga86400acacctgagc tctggcagtc cgcctgcctc agcctctcaa agtgctggga ttgcaggcgt86460gagccaccat gcccggctgt tcagttttat ttttaaactt tgtaaaagta ttatgtgatg86520gttacataag gtgatataaa ttttgtagag cagttggaca tgcctagaaa agttgggggt86580acattgtgat ctagtaattt tattagtagg tatatgcctt aggggaactg ttgcatgtgt86640ctaccagggt atatgtacaa aaagttcata gaagcactgg gtataatttc aaaaagggaa86700gtaatgcaaa tatccatcaa gaggaaaatg aatatttaat atatggtctg ttcacataat86760ggagtaaaac acagcattga aagtgcatga actgtaaact gaacaacaca gatgaatctt86820agaaaaataa tgttgaaaat ggcatgataa attgttttgg aattgtaagc attgcaatca86880tactagaaag ctactttcca aagtattgta gggtctttaa atcaatcagc aatatggaag86940gagatgactt aggtttacaa acttaactgc actttgagct ctgcattaca catctataaa87000tttaggctta tgacacctaa ttcacaggct gttttgatat ttaaatgaac tactataata87060aatgcaaaat aatttataag tacaaaacat tactcccgta ttatttttgg ggggtcaatt87120tcatggatac tctatagtcc accaaatttc tactttttgt gaaaatattc caaagtgaag87180catcagttcc acaagcatcc ccttgtatgt ttaaaatttg aagattcttc tagaaaaact87240tgccaaacat tcttaggata aaaggaaaaa atattgcaca tatgaacatg cctcaggtaa87300gtataatctt tgaagtttaa gaattaaaaa ccataacatt ttaatagaac ttcctctaaa87360cattccttcc tcttagacat tgatttaaaa catatttgct tttaatattt ctccttgttc87420atatgcaatt ttacacttct aaactaaatg aatagtttta atcttgattt cctactgtca87480gctttcctgt tgtatttctc ctcttgtttt ctatgagtta cataaatgaa actgacatca87540ttgctcatac agcaggcata tttttttttg cccccaaatg ctgccttttt gctattcagt87600gtatctggat accagctaat gctaaatgaa gtttgacatg tacccctaag aaacaaaact87660tataaattgt ggcttcaaag gaaaccttga ataaatagga cacactgtgt ccacccaggc87720taacatccag catatgtttt agcttcctga tgatgagata gagcaaaata gcccccagga87780aactttgcaa ttgtttattg ctcacttgcc cagcatgtat taaataccag gctttcattc87840tcttataatt tgcttttttt aaaattaaaa tccaccaaag tagatttgat caggtatagc87900aagccgagat agtagtcatc catatttgta ctctgtatat aaaagcttaa agacagtgct87960aaaaattctc cagtagtagg atgtatgtaa gttgtttgaa cagatagttc tagtgggttc88020atagtaagct atttacctct cccattctgt ggttcagttg acccatttaa aaaagattca88080ggatcaaagg cagaagctat tgtatcaaaa atctttgtcc actcttctct tgatttactc88140attcatccaa aaaatattta tggaacctct actatttgcc aggcattgta catatatgcc88200atatattttg ccaaatacat ggtccctacc accaccaatg ttttacattt gtgtagcaga88260gtgtggtttt ttaagctttt tttttttttt tgcagaaatt atctttgaac taactttgta88320tgtatgctgg gataggatat tatgatctct attttacagg aaggaagagg aatcatagct88380aaaaatctac attgatagtt tagatacatg gggatgtgaa gataatttta ttttttagct88440aaaaagagga ctttacaaaa tgaagattat agcatcatgg cttctttcag ctatgattga88500tttttttttt tctttttaaa gactttactt ttttagagaa attttaggtt cacaacaaaa88560ttgagagaaa ggtacagaga cttcctcttt cccccctgct cccatacatg tttagactct88620cccactatca acatccctca ctgcagtgaa gcattcgttt cagtcaatga acctacatgg88680acacatcatt atcacccaga gtccatagtc tacatgaagt ttcactcttg gtgtacattc88740tgtcggctct gataaatata taatgatatg tatccaccac tgccatatca tactgaatag88800tttcgctggc ctaaaaatcc tctgtgctct gcctagtcat ccttccccct cccctaaccc88860ctggcaacca atgatctttt cactgtctcc agagttgtgc ctttcccaga atgacatgta88920gttggaatcc tacagtatgt cagctaccat tgatttttaa gttcgttata cacttttaaa88980aagtagccaa atagttcaac aaaggctatc caattttatt atggaaaact caaatatatg89040caaaattaat gagaataata aaattaactt tcaattttag cacttataaa ctcatgacca89100aacacttata aactcatggc cagtctatac tctcatttcc cctttccccc actccccact89160ataacaaatg ttttatccat ggatatttag tctgtatctt taaagttaag aactctcttt89220caaacacagc cacacctaaa ttgttcaaca ataatttctt ttctagatag atagatagat89280agatagatag atagatagat agatagatag atattttatg gagatggggt cttgctctgt89340tgcccaggct ggtcttgaac tcctgggctc aagtgatcct cccaccttgg cctcccgaag89400tgctgggatt ccaggcataa gccactatac ccagccaaca ataatttctt aatatcaaat89460atctggttat gttcacatgt cccttataat ctcataactt tattaggctc ctgattttaa89520aaaccaactc ataaaaaata gacaaaatct tacatataag ttgattcatt ctttttattg89580ttgttattat attttagatt caggggcaca tgtacaggtt tgttacatgg atatattatt89640gcttaatggt gacgttgggg cttctagtga acccatcacc caaatagtga acattatact89700caataggtag ctttttaact ctcaccctct tcccacccac ccccaccttt tggagccccc89760agtgtggatt cattcttttt taactgatag gtttccagtc cctttttttg tttcctgatg89820
gttttatgta acagtttccc tttgatgtaa attggtagac ttgtctcatt aatctgttgc89880tgcataagaa gcaacaccta agctcagtgg cttaaaacaa tgattggtta tttctcatag89940ttctgaggat ggatgggctc agctgggtgg ttgtcacttg gggtccttct atatagttat90000agtgagatgg cagctggggc tgaagtcttc taaaggcttc tttactttcc tgtttggtgc90060ctggacttgg aaggctgggg tggccacaac ttggtctggc acctctttca ccatggcttc90120tctgtatggc tagcttggac tttttcacag ggtacagact tcttatgtgg tggctggctt90180accccatgag cccaccagga gaccaaagca gaaacagagg gcttcttttg acctagtctt90240agaagtcaca cagtgtcact ttcaccacat tctgttaatt aaagtaaatc acaaaagcag90300ccaaacttaa aagagagggg attacaaaag taagaaaagc agaaggcgta gtttactgag90360gagaggtggg acatatttgg tgactagcaa caaaaaggct agatcaaatt gttttgtcaa90420aaatacttta tgggtgatgt aaatttccat cacaagtctg tttacctctt tttaattttt90480ttaaattagt aattataatt attgcctaga taattacagc atttcattag aggttaaggg90540gttataaaat gttactttta taattctatt tttccttctt tatgggttag ttggattact90600tataatttcc ctgtatcaac tatctgccta ccttaaggta tattgtatat aggaaagtct90660ggataaatgc ttgattcttt tcatttattt gtactcaaaa ataatgagtt agctttttta90720gtatcctcca aatgtaatga gatctttttc tgaggggaag cttgtcaaca taaactcatg90780aatttgaagt atttgataga tacatgtcaa tccatcagaa ttattgatgt tcatattgcc90840catctctggc cctggggcac ttcctgcagt tggcccctga gcccttttga taccctgtca90900gtggtctatg atgatttctg tgctgtttgc tatgacaaga tactccggtt tcatcttgaa90960actttccttc cctagttttg gaagcagcca tttcttcagt aacacttgac tccctcatgt91020ggaaaatggt aactagggac cataatctat ttgctatgaa ggttcattgc tactggttgg91080tcattatttc taggcctttc aatagaaaaa attaggaaaa aaattatgtg aagaagaaaa91140acatgttgag tttttagata cagttccact tcaaatttag tattacatgg tttttattta91200acgttatcaa tcagacttcc aaaacagttt aattttattt ttcagtttcg ttgttgaatg91260gggacatacg gacaaattac tgtcttttaa agtgacttaa catatttatt tcctataagt91320gtggttatgt caccacttaa tacaacagat aatttcattt tgctttagat gattaggaat91380ttattttttt aaactgtgct tctgttttat aaattatgta aataaagtgt tttaaagtaa91440aaaatgtaaa caaagacaat tcaaacaagg ctagcttctt tccttgttct gccctattcc91500ctccttcttt aaaattaact taaaaaattt ttttaggatt ttggtatgtt cttttaacgt91560atgtatgcat atgtgtttat tatatgtaca cttgtttttg ctctgcctac cattttgtag91620gtggtagtag tgccatgctt ttttcaccta atagagtaga tactgctttc cataagtata91680agaggccaca aaattttaaa aggtgaattt aaaagcaggg accaactttt atggaattac91740atttcatagc catgttaact gcttagatca aaatatacct gctcatacag ccttagccat91800caataaacca cactttcaaa ttatttttct ttttataagt gaaaataaaa tcttatttcc91860tttctttgga taccacaaaa caatgacaca tccactagtc tgatctgtgt agagagctta91920aaattaatca gagagtctca aatgctgtta ttatcattat gtaaaatatg ctgtgatttg91980ttcaaatttg ctaaaatctg gttcataaca tttgcttgca ttgaatgcta atatgcttag92040tcagactaag tgcttctgtc aattatagtc ttttaaatag atgagtctgt cttatactaa92100tatgtgaaat tatacttctt ggtaaaattt gtgttgatat gctttatatt aatggtgcat92160tttttaaaat ttagagataa agctaattaa ttgtagttta agatataaaa atttgaggga92220ctttctgctt ttgtttcaaa gacattcgga gaacacatgc ccaagaaatg taagccatgc92280ttaaaaatac tgcattttga agcttgcagt aactatctaa tgaattcgtc tcagagtgat92340tctccttcag aaaaacaaaa ttttataggt cacatttgga aacaaggaga ggaggaaata92400actgacattg caatatgggg aaataaatgg gaaataaaag gccataatga ttggtcttca92460ggaagctgta aggattgttc ttcaggaact aatttttctt gctactttat tgtgtggaca92520atataatgcc tcacgtatct gttggcatag tccctagttt ctctctgctg tacccattct92580tcacatattt tctagcattc tttcaagagg atcaggagtc tttgcccaga tacctgtttg92640ggaaaaggaa gaaagagagt aaaaaattaa taattaaatc aattttggag ttattctgca92700gatgacacac agcagctttc ttagtgtaat ggaaagccag cctggtatta agcatctaac92760ctcacaaaga atggcagtaa taaaaatatc tcttcaatta aaaagcccca cactttagtt92820tcaactacca agccttggaa atgtaataaa aaaaaaactg tgaaaaaaag tcttcggtac92880atagttttta taaatataac taacaccttg gtgtagatta tattctctgg agccaaatag92940attactattt tagaatgtga agtttcaaac tagtttcatt attactatta atacttactc93000atatcacact tacattagta aaaccctctg tagatcatta tatccactca agcatgaacc93060acacaacacc tatttttttt aactcatctt tgcacattaa aaaacataca ggcacacgca93120actaacctcc cacacaagtt aaataactat tgctcctttt ctgttcaaaa ccaaatatat93180actcttgcat tcactggaaa gtgttactag tatatgattt gctatctatt cagaagaata93240gatatttgag aataaaataa aaaagtatat aataatgaaa gcaactacca aattaaaagc93300aatatcaaat aatttgtact tgatattttt atgtcaaaga tgattaacat gtaactgatt93360tacctgggat agaccaggta aatcagtaca ccagaaactt catatttccc aggaatttta93420aattttctta gtttttaacc cgagaaatcc tacaaagaat ggatttggtg atttgtgttt93480tattttcctt aagaaggttg aagtctgaaa aaggtaactg agaagcagct cacaggcagt93540tctctctttt aataattccc cgtgtgcaaa gcattgtact tcaatagtta cctcagaacc93600attttagtga aaaagaaggt aaaaatacat tatcattaaa aaactaatct gaatatagtc93660aggatttaca aattggataa actgactact ctcattctaa taaaagaata aaataaaccc93720aatttattat tttcaatagt tgcctcttcc aacagcattt aaattaatag ataatagtcc93780acgctaattg taagaaacat gtctattgta taaaaccgga ttgaaacatg tctattatat93840aaagccagat tgatatgtgt atttttgcct ttttcctctg tcttctactt gtatatttct9390attttctcatc acagattttg gtaaaattgg tattttcatt atccttaagt atgagcaaag93960agagataaac atatttctcc actcaattat gtcaattttt ttcttttttt cttttattta94020tttatttatt tatttttatt attattatac tttaagtttt agggtacatg tgcacaatgt94080gcaggttagt tacatatgta tacatgtgcc atgctagtgc gctgcaccca ctaacttgtc94140atctagcatt aggtatatct cccaatgcta tccctccccc ctccccccac cccacaacag94200gccccagagt gtgatgttcc ccttcctgtg tccatgtgtt ctcattgttc aattcccacc94260tatgagtgag aatatgtggt gttcggtttt ttgttcttgc gatagtttac tgagaatgat94320gatttccaat ttcatccatg tccctacaaa ggacgtgatc tcatcatttt ttatggctgc94380atagtactcc atggtgtata tgtgccacat tttcttaatc cagtctatca ttgttggaca94440tttgggttgg ttccaagtct ttgctatcgt gaataatgcc gcagtaaaca tagtgtgcat94500gtgtctttat agcagcatga tttatagtcc tttgggtata tacccagtaa tgggatggct94560gggacaaatg gtatttctag ttctagatcc ctgaggaatc gccacactga cttccacaat94620ggttgaacta gtttacagtc ccaccaacag tgtaaaagtg ttcctatttc tccacatgct94680ctccagcacc tgttgtttcc tgacttttta atgattgcca ttctaactgg tgtgagatgg94740tatctcattg tggttttgat ttgcatttct ctgatggcca gtgatggtga gcattttttc94800atatgttttt tggctgcata aatgtcttct tttgagaagt gtctgttcat ttccttcgcc94860cactttttga tggggttgtt tgtttttttc ttgtaaattt gtttgagttc attgtagatt94920ctggatatta gccctttgtc agatgagtag gttgcgaaaa ttttctccca ttttgtaggt94980tgcctgttca ctctgatggt agtttctttt gctgtgcaga agctctttag tttaattaga95040tcccatttgt caattttgtc ttttgttgcc attgcttttg gtgttttaga catgaagtcc95100ttgcccatgc ctatgtcctg aatggtaatg cctaggtttt cttctagggt ttttatggtt95160ttaattctaa cgtttaagtc tttaatccat cttgaattga tttttgtata agctgtaagg95220
aagggatcca gtttcagctt tctacatatg gctagccagt tttcccagca ccatttatta95280aatagggaat cctttcccca ttgcttgttt ttctcaggtt tgtcaaagat cagatagttg95340tagatatgtg gcgttatttc tgagggctct gttctgttcc attgatctat atctctgttt95400tcgtaccagt accatgctgt tttggttact gtagccttgt agtatagttt gaagtcaggt95460agcgtgatgc ctccagcttt gttcttttgg cttaggattg ccttggcgat gtgggctctt95520ttttggttcc atatgaactt taaagtacta ttttccaatt ctgtgaagaa agtgattggt95580agcttgatgg ggatggcatt gaatctgtaa attaccttgg gcagtatggc cattttcact95640atattgattc ttcctaccca tgagcatgga atgttcttcc atttgtttgt atcctctttt95700atttccttga gcagtggttt gtagttctcc ttgaagaggt ccttcacatc ccttgtaagt95760tggattccta ggtattttat tctctttgaa gcaattgtga atgggagttc actcatgatt95820tggctctctg tttgtctgtt gttggtgtat aagaatgctt gtgatttttg tacattgatt95880ttgtatcctg agactttgct tatcagctta aggagatttt gggctgagac aatggggttt95940tctggatata cgatcatgtc atctgcaaac agggacaatt tgacttcctc ttttcctaac96000tgaataccct ttatttcttt ctcctgtcta attgccctgg ccagaacttc caacactatg96060ttgaatagga gtggtgagag agggcatccc tgtcttgtgc cagttttcaa agggaatgct96120tccagttttt gcccattcag tatgatattg gctgtgggtt tgtcatggat agctcttatt96180attttgaaat acgttccatc aatacctaat ttattgagag tttttagcat gaagcgttgt96240tgaattttgt caaaggcctt ttctgcatct attgagataa tcatgtggtt tttgtcttta96300gttctgttta tatgctggat tacatttatt gatttgtgta tattgaacca gccttgcatc96360ccagggatga agcccacttg atcatggtgg ataagctttt cgatgtgctg ctggattcgg96420ttttttaaag tagcaaacaa tgctgtttgt ttatttttta tgtttttaga gacagtgcct96480cactttgttt cctgggctgg acttgaactc ctgggctcaa gtgattctcc cacctcagtc96540tcccaagtag ctgggagtgt gggcatgcac cacggtgctt tgtttaagac tctattttca96600aaaaaccaag cataaacatt attaacacaa acataatgaa atacatatac aatttagagt96660aatatttaaa gtgatgtaaa tatctagatg cgattaaaca cttcactcac tgaagtttaa96720ttattttaat ttgctacatt tgctgtttaa tgttactttg gtcttctctt ctccccatcc96780catcccaaat atgcatagtt ttaattcacc cctgccccct ttaaatatat atgtatttaa96840aatccacaga ttggtgttag tgttgggaag ggggatttct gctgaaactc cacatgtgct96900ccttactgct ggttgatgct tttgatgttc tcatcccctg aggtcaggag aacacagaca96960ggagagtaag tggcaactgt agcaacccca ggctttgtga cacttctctc cagaagatgc97020aagggtattt tctgcacatc agcactggcc ctgacagtag agagtttctt ttcatttact97080ttaaaatggt tctgacaaaa accggggtct ctcatttgca aaaccagctg tctgtagcaa97140agccggacct tatcttaaga gccaggctga ggccacagat gtcgtgtggc acctgcatgt97200ggaggccacg agtggcccat taaccagatc tgggctgagg ccactgagtc tgcaccatgg97260agctcactcc tggaggccag ctgcaatatt cccacctggc caggaaggac actgcccttc97320tcagtcccag aatggtaatc tgagggccct ccacaacaca atggggcctt cagcctaggc97380atggaccact gtggtgcttt ccctctctcc ataggacaaa tggagggcca caggttccag97440caatgcctgg agcccacttg gtcctttaat atcccatctt gtcacttctc aggcccctgc97500ctactccacc aaaagggctg gggaggcagt ccaaagatag gggttataga tttatgttaa97560taaatatata atatatatta atatgtaatt ttatttttat ttttactttt ttaagtgaca97620aagtctcgtt cagttgttag gctggagtac agtggagtga tcacaactca ctgcagtctt97680gacctctgga gtttaagcaa atctcctcag cctccgacta gctaagacta tcgttgtgca97740ccaacacatc tggctagttt ttatttttaa aaaactcttt gcagagacaa ggccttgctt97800tgttgcccag gctggtgatg aacacctggc ttcaagttgt cctctcacct tggcctccca97860aagtgctggg attacagatg tgagccactg catccagccc ccagatttta atttagtttt97920attttctacc tttttgtgat tatgtgtctc agtttaacaa gcctttagtt acatctaggt97980ataatcaagg gccttctgtg tgcccagtgg ttatctatga ttggactttt tctcttctcc98040ccatgtgctc ccccaccatt aaattttaag gaggtacttt ttccttgtta cctgcacttt98100ttgaacaata ggtttcagac aacttaccct tgtaaattcc ctttgaatta tggcatttta98160accatttttc acaaatattg aacttgaaat tttagagcca tcaggagggg ctgatcctag98220cagctctttt attgaaatta ttctattggt ggcaatagga gttactggag ggcactgcca98280cccagaaact ggatgaaacc aagtcagaaa ttctgagtat aactgacaaa tgctttatag98340gaatttagaa ggacattggg tagtagaggg cttgggaaaa agtaaattcc ctaatttcct98400cctctgtgac ccacatttga tatagaaatc atgatttgtc tagtattgga cagaatcaca98460aataaggatt agttgccata aaagtttttc taatttatat tgtgaaagag ctgtagtgct98520gcatcagaaa ggagtctctg tttgctagaa gagctatgtc agcaggtgag gcctaaagcc98580taaaggcctg aggggtggtc agggatggga gagaatgaag ttactccaga ctttcaaatt98640gttcctcacc cttcacaaga cgggaagttt cctaaggctg aaggaagcag cagtcactga98700gtgcagaatg tttctcttgg tgcagtgggt tgtgaccact ctcattgttg ttttcttcct98760gtccagagtg aaacaggtat ccttcagaaa tttcatagca tgtagccctg aagagcactt98820agctagattg tggtggtttc tatatgtgta attggttaat ctgttgaaga gaaataatga98880aaagctgagc agtctttttc tcctccatca actcatgagc tatgagctat gagttctcga98940gaaatgttgg agcatttgac agcagtattt gaattctccc ttgtgtttct gttgctgaag99000ctgcagcttc caaaattgca gggcttcttg accagcctgc gtctcaccat tcagcctttt99060agtccattga ataatctgat tctgtcagca aggcctcagg tttagatctt attagctcta99120gatagttttt taaaaatctg ctctccagaa atttgcagga agtcccaaga taagagaaaa99180cttcctgtca acagtgctga atttcatggt gtgtgattat tgggaagaag ttgactgaat99240gttgatatgt gatacttctg aattgaggtg tgcccaatgt aggtctaggc atacattata99300ctgttgctgc atgtatgtaa aattttaaaa ttggaatggg agagttctct gacccccctc99360gcaggatgtg caatgggtgt ggctcatctg ttcggccacc atgctcaaac cccttatggg99420agggggagca cacagaaggg cagctgcagg agccagggtg agcgcttttg ggctccagcc99480cacggtagcg tctaggggtg ggtgtctgtg actcctgaag ccccagtggg catgtcacaa99540tgctccttta gctctgccat ctgcagaaag ctaaagcgtt aaccagctca gtgccctctt99600ggtacccagg ttcttgtctg gtatccagga agtatcaggt cacacagaca aattgaagga99660tggtaaatgc ggggtgtctt atttccaggt ggaggtggct ctcagcagat ggatggggag99720ctagaaaggg gatggagtgg gaatatgaac ttcgcctgga gtttggccgt cctgtggccg99780atctcctctc tgaccatcca cagccaaact cttcttgatg tttaaatgcc ctttctcttc99840tctcctctgc tgtgccactc ttctgcttct ttgctcttct gcttatctgt tatctgctca99900tctgctcgag gagcctgggg tttggggttt ataatggtac acgatgcagg gatgcagcag99960gccaaaagga aatgtttggg tgcaaaaaca ggaatgcctg ttcccattta gggccatggg 100020tttccaggct tgagggtggg gcctttgcca gggaactgcc ttcttctacc cagtatttcc 100080ttgcctcctg tccatatcaa aatgagtatc aatactctgt aagattttct tttgtttact 100140tctgtactct attcttcaat attagattta atgtattagt cttgggattg gaatgactat 100200aaaggaagca gcattttgta tagtaagttg aaagaatctg taggtttata gctttctaag 100260taagatagta ttctcagttg ttttttcaca tgactagcat ttacatttat ttatgtatcc 100320agtaattata tgaataccat ctataagtca gctaatgtga taggtaatgg agatgaacag 100380aataacatga attgcttttt cttaaaaaaa aaaaaactct aacagaggat acagacaagg 100440aaaccattga tttatggttg cctgtgagaa gttctgtgac aagcatatac ctaatatggg 100500tatgtgtgtg agagaatcta ggaaagcttc cttaagtaag taatgctttg ctaaatttta 100560agaaagggta gtatggccag gtaaagtcag tgggggagga agatgacagg gaacactgtg 100620
gccaaagcat gcagacccat ggaattacag gaagcagttg tgtagagctt gagtgtaggg 100680tggtgtaggg cctgaatcat ggaggacaag gtatgccagg atgaggagtt tagtttgaat 100740tctctaggca atgggcatcc agtgaatgca aacagcattt gatacaggta ctcagggtgc 100800agaaaactga atgattaata ggagggcatt agatgagatt ttatgctgat gggaaggaat 100860cccaggtgag agagagagag agaaagagag agaaagaaag agaaaaagac ctgggaaaga 100920gagagagaga gaagagagag aaagaaagaa agagagagag agcgagaaag agaaaaagac 100980ctgggggggg gcggggagag agagagacct aggaaagaga ggggggtggg gagagagaga 101040gagagagaga gagaaagaaa gacctgggaa agagaaggca tattacatgg gccattatcc 101100atctgcttgc tttaatttct tggacaactc cagcatctac tacccagatt ttcttgttgt 101160tgtttttttt tgttttgttt tgttagttgt ctttgtggct tacctcttta tttgtgtagc 101220cgccctttct tgtcttttgt tacatctttg atttggaagt gaattaataa atgaatgtca 101280agtgttccca aattctgttt taagaataat ctagatcttt taaagcttcg gtaatgtggg 101340ctttaaaaaa tctgtgattg cccaactggt ttatgtcagc attttggaaa gaatatcagt 101400ctttaaagcg gtgttagaga tataaaccct ctggggagtg aagaatgcct ggtgcatgct 101460aattttgtac ttatccaggt ggaagagaga gaggaatagc caaagctata gatagaacaa 101520aggcatgtca attgcaacct ggtctcagtt taggcttagc tctcctcacc aatcccctat 101580caggatatta gtggcttatt tatttatttt taacctgtat catattaagc tctttgacaa 101640atcgcatttt gatttatagt ctgtgcttct ggcatgtgct ttaatggcat tagccaactc 101700tagacacata ctgaattcat tatgtacctt atgtatgtaa gtagtaatac ttccttttaa 101760tgaacatacg gtcacgtcaa agtgctgcga ggttgtttta caaaagcatc tttgtgtagt 101820gctcctcaga agttttgaga tgagtattaa gttgaagatt aatatgctgt attttaagtt 101880gtattttagt ggagtaatgt attataattt tgccctctcc ttaaggtgag tagactatta 101940tagtctgagt ctttgattct taggctttgg ttcttaatta gaattcaaca ttgttgacat 102000gtaaattgtg accagatgaa ttcaaaactc aaaaagtaaa attgcctttg aatttgtatc 102060ctcagctacg atattgattg aaaatcgatg agtaactctt attcttgttt tctataggca 102120gtaagaaatt cacagaatct atttacagct atacgtgaca gcacttaacc ttgactatgc 102180ctgctaaaag gtttcttgac tttaaaatgt ggatactctt caagatttga gggcagggct 102240tctcctaagg tagaggcaga gaagtaaacc agcaacaagt agggctcaag atctagcctg 102300tctgatcagg cttccagacc aaggagtggc agcagtccaa accacctgtc tctgctgact 102360aggcaatctt aatagccatc ccactcctca tcagtacgtg ggatctgagc acaggtctaa 102420gcagattaat agttttctta gtgaagtgat ccatggagtc aaatgcatga cagtatcttc 102480aggattctgt gggaagagga gggggtctat ggaggacact aggtcatccc cttgaaaaga 102540gagatagaga ccattacttg agggaagagc taagcagaat gtcagagctt tggctaagca 102600aacagatatg agcagagttc tagttcaact aggagcacag agtgagaacc agatattggg 102660gcaggaactc ttctgtttga cgtctacagt gggagagact gcacacctgt gagcacaggg 102720attcctttgt gtaggggttc cttaaagaca gataacagtt tatgggcaga taacagtttc 102780cagagggatg gggctccctg tataatgcca ggggctgcca ggattgactt aggattgact 102840cagggactgc gtggggctga ccctgcaggt tgaatgctgt cagtgattac aacagggcaa 102900gggcaccttg actgtctcag aaactaatag aatctttctt tgacaataac cactggcgag 102960catttccagg aagtctttga aaattgctgg gagtctgggc aagaaactcc actcactgaa 103020agcatggtat ttgattcatt ataaaacaga aggaaaaaca ttagtaatag acgtggtatg 103080tcccagcctt gtatagttga aagtaaaact aagtcatacg ttttcaattt ggtgtgacat 103140tgtctaattt ctaaatttat tattattttg aaattatagg aaatgattag aagtatgagg 103200gtgtgcttct tctgttgttt atttttcaca tttgagtctt actacatgtc accattgtcc 103260agcatttcag agacaaaggg agtatttttg acattcatta aaactgtttt ggtgaaagag 103320ctgtattacg gtactattta atttttataa tagtttatca gaaatgagat tagagcctat 103380ttattgaatt atcttatttt gaaactctca catatttatt gagtacagat tagaatcaaa 103440ctgtttagaa tattagaaaa gtctttttca agtacaaagg gcagagatta tatctgtagg 103500tagttccccc aaacccccga ggggaagttt tgttttaaat gtttacatgt tttatattga 103560cacatttgtt ggcttaaaaa atatggcaat agcatctagc atagtgctag atgtatgtgg 103620ttgcaatttg acattatttt attgataact atattgtgta taaactttct tttcatgtct 103680ttttatgtga gagacatcag atattctact agctgttagg aggtagtgaa attaagttga 103740ttctcacagt atttgcatct cagtcttctg cctcagcatg tgaccttgcc tgtgaaggtt 103800cagaatagct gttttattat ttcgcttagg gtgatcgggc tctggtaaat tgattggcac 103860tagcccaact cacagcctat tgaatcatga atttattact ttaggcaaca aatagtttaa 103920caccttccta catgccatac attgttgtcc ttgcaggagg taaactcttg gagaagatgg 103980tgggggatag aaccaagttc ctgcccttaa tttattcatt cctatgagaa gtgaaacatt 104040catgtaaaac agatgactca gattgctgtt aaaaaaaaaa aaaaatccag tagggcaaac 104100ctatttgaat aaccaaatta agatcaggat tggctgggtg tggtggttca cgcctgtaat 104160cccagcactt tgggaggcca aggtgggtgg atcacgaggt caggagattg agaccatcct 104220ggctaacacg gtgaaacccc gtctctacta aaaatacaaa aaaattagct gggcatggtg 104280gcgggctcct gtagtcccag ctactcggga ggctgaggca ggagaatggt gtgaatccag 104340gagggggagc ttgcagtgag ccgagatcac gccactgcac tgcagcctgt gcaacagagc 104400aagactgcat ctcaaaaaaa aaaaaaaaaa agatcaggat tttgcctagt aactatagag 104460ttcaagcaga agcaatgaaa tggtctaaac tatagcttaa aaaacaatat ctgttaaaga 104520tatctttgtc aagtaagtct gaatattttg cttttatagt taaaaagata attaaattct 104580gactttttca tttcatcttt tagagtctca agccccatat aaagtttcca aataaacatt 104640aattctgggt tgctgagaga tgttattctg ataactgagg aatgtttatg attgaggtat 104700taaataaaag gttatcttaa caaaaggaga aggaaggtaa atgtagtaga aggcaatctg 104760ttaacaatat tagcttcatt cttataatgc tttgcagttt acaaatgctt tcacatatgc 104820tggaatctga ttatttcagc ggttctaggg gattggtaga gaggctgttg ttagacctac 104880ttaatagatg aaacagactc tgatgaggaa agggacgtcc ccagaacctg agttagtggc 104940tggttgggtc ctgaactcac acattcggag tctccagcat cagtgctctc tgatgggggc 105000caagagttag cctgcttttc atgtaaatgc cctagtacac attcctttca gacaatcagg 105060acattgggtt caggtcagac tgatttgata acaatttgat aaggtgtgga atcttcctgt 105120gattaaagag agaaatctga taaggtacaa gacttaccat gaaacagata aaggctctct 105180caaaaggctg cctccagtta gaattcccca gctggtcctt ggcacatggt tccatgtgtt 105240tattatgtag ctcacactcg aaatcacccc tggggcaggt tgagccattc cagcattttg 105300taaaaagttg tgtgcatgtg ctcacacaca aatgaggaaa agaggactga aaaaaatggt 105360tctgtgaaag tgaaaggatt cacttgcttt tcccagtcct attatgaaag atggtttagt 105420tatatcaaag ggcttctttt gacttgttgt ataattcgat tcttgagttt tcagttggct 105480tatttgaaat tttgtaaatt gcaatttata aaattggagg gccctgagag atcaagttcc 105540acttgtggct cgaggaatga ggatactgag attaggggtt agggcttgct caggtttttg 105600ttgcagacag gaaaaacaaa aaatctggtt aacttaaaca gaaaagaaat tctctgatag 105660gacattgagc agcaggcaga aatactggga agacagagac ctagcttgaa aaataaggga 105720ctaaatatga tttgtagcaa ccacagccac aggcaaaatc ctgacccaca accagtgcac 105780cgaagacttg attgctcatc acgtcacgtc ttgctgtgcc accactgctg acatctctat 105840tgctgctgcc cctagaaatg ggatggtact gctatcattg catctacctc tggattagag 105900gttctttggg tcactacctc tagatcgaag actgttgaga gcgtcggact ggcaaagcca 105960ggttaaagtt ctgtatccca ggggaccgtt cttcctctcc tatgggatgg tagaatgcct 106020
ttctgcaaag cttacaagat aggaggaggg gtttagacgc tagtaagcaa aaccaaacca 106080taccaaaaca tgagacagtt atctgctatg gaaacatacc catttactgg ctatttaaga 106140ggttagaact tgagttttct gaattttctc aggatgccaa atttatagct aatatagatt 106200tcctttatga aaagtgacag aggctctgtg gtttgtaaga gaaaatgaag tcctctgtag 106260taagatttaa taattctaat attttgaaac aatccatttt cctatcaaca ctgttttcct 106320ctggattaaa aaccattccc catcttccta tccttcatga catacagaaa aaagaatctt 106380atttttcagt ggtaaaaagt ggagagagag aaaaggagta aaagaagaac aaacagtatt 106440catgcagtgc agagattaga ggttagttgt tacacatttc agggtaagtt tgccagattg 106500atgagcaata aaaccattta gaactggaaa atcttcaggg cttttgtttt taaaagttat 106560tttaggcaat aaccacatct tgtcgttata gtctttgctt tccagcaatt cctttttacc 106620ttttaataat aaaagctaat atttattgag aactattttg tcatagcatc attatctatc 106680tcacttcttc ctcacaataa ctctgtgtta atctcattac tattctcctt ccacagctga 106740gagatcaaac cttagatgaa gcaactagca tgagatttca tagttcatag atgtgggact 106800tgaacttaag catatacacc tgtgaaagaa gctttgtaaa tctttcttta acttaagaga 106860gaaaataagg tatgtgtaga atgctgtttg gtaatatttt aagaaaactg cagaggaaga 106920agaaaggaag tgtgctagaa aatcaaaaga cttttgggag catgtgactc ctccacatta 106980ggtaaatttg ctcttgtatc tctgttccag gacagctagg ccattctgat ctctttcaat 107040ttctgttccc cacagtagta cttagcttag tgcctggtgc atagtgggtg ttcaataaat 107100atttacaaaa ataattaatc tcaggcatcc tcaggacagt ttcctgggct taagatgcca 107160tgcaatttag agtgctgctc tcagtaaaga aataacttta ccctcaaagc caggcatttc 107220aagaaggttt atcaatgaat ttagtgcatt tcacaaacaa ggaagcttac caggggtttt 107280taggccatat aactgcatag cctgtgaaag atctctatag catgtcttgc ttcctgactt 107340tccaacttca cttcaattaa ttttgtcaca ttgctccctt ttcctttttc ttccttaaac 107400gtataaaatg ctgtttgttt tttttttttt tttttttttg gctgtggtcg agagagaatg 107460gattgtcctg ggaaaggcac aaggaatctt tattgtaaac taatgtgtgg aacatttttg 107520tacagaaata gctaatgaat acccaaagca tgctgttgct tgctgtttaa atgtggtttc 107580atttttgttc agctctagct tctaagtgtc ttaaaaggag aaaacttact tttcattaga 107640ttcatttaaa actttttcaa gagaggaaag tcctgagggt atagaaactt tatgtactta 107700aatatatcat gttaaggaac ttcacaatgt ttcgtttata tggggccaaa gaccacagat 107760ccaccccgat tgctaattat tgagattgca aatagcttat ttcagaactt agggtgagac 107820aaaggaaaca tgaaggaaca aaaggcccag gataatgaaa aggatattgt gaagagaaag 107880gcctgtagaa ttatgatgca ggtcacatcc tgtgcctgca tttaattgta cctttgtatt 107940taataataaa aatgattaca tgctcactgc taacaggtgt ccctgcttca gaactgtagt 108000atggataggt gacagcaaat tactccaatt cataaacttg gaatattatg cttttatttt 108060gagtttcacc agtctatact agaatacatt ttctgccaaa atgtacatca acccaggaag 108120atttttaaac ttagcccctg ataaatatac ataaaacata ggtgaatatt ttaaagtata 108180aatgaaagaa aaaatcaact ttgccaggca cttaacacat tgaggttgta ttaataccat 108240agtcacattt ctagttcttt atattttata taagctttga atgtaatgta aattagcagt 108300gatgtaagtg gtctttctgt tagaacttaa ttgcctcctt tatgaatttt ctctaattct 108360tgcttaactt gaacactttc tgtaactgcc tgtccaaaat atagcccctg ccctagggat 108420ttgttctcct ctgtttttcc ctttattgcc attattgcaa taacaatgtt cttattaatc 108480tgtttgattt tatctgtctt cctcattaga atgaagttcc ataggacaga gaccaaggta 108540atcttattct ccctaaatca gtggttgcct agtaccttga ccatgcctgg ctcataatgt 108600gtacccccaa aaatatttgt taaagacatt cgttaatctg ccacttaccg tcttaatcat 108660ataccaaaaa ccctgcttgc taccttaact ttatcactag cctcctccct ttgcttatgt 108720tttcaccaaa gattttgtgg ttgaagacat tctatttcca ttgtctgttg ggtagctagg 108780tagagagata atagtccccc ttgatcccat gaccatagtt gaatggaaat tgaggggttg 108840gggactgttc ttagactctt taagccttga gcacatttcc tttatttgat cagcctctga 108900actgtgattc attcatattt gaatatctgc taggtagcaa aggctttggc aggtactggg 108960gaagcaaatt tgaaaaagat gcactctgtt cttttaggaa catatcatta gaattgatgt 109020cccataagtc tgtaactgtg tctctttggc tgtgcattat agactcagtt cctagcacaa 109080tctctggtac attacaagtg atcaataaat gagtgaatat tccatgcaag tatttagaaa 109140atagcttgtg ctgtaataaa tgtatgtaca aagttctctg gaaacacaga agagggaagg 109200ccattgtgtc taaaaggagt cagggaaagt ttcacaaagg aaggattaaa agttgagttt 109260accagtttgg gtaagatgta ccacatagta aggacaacat gagcaagaaa tcagaggtgt 109320ggaagagcaa tattttattt gagtttgggc actgcggtca gtctggaaat ttaggacgaa 109380tgtgaaggag tggtgggcat tgaggctgtt ggaatcatta tttattccct tattcaggta 109440ccctatgcac taagtagctc ctgtggcaca aacaaatcac cttttattta tagtatttct 109500atagaaaaaa gaattgagtt ccaaacatac agttttgaag atagatcctg tttataaatg 109560gggattaact ttagtagaaa attggctaca ttgcctatat gttatctatt gctgtaattc 109620ttcatacaga taaccacaaa cgtgaatggc ataaaaataa ttagggttta ttgtacatgc 109680attggaatgc tcatcaggtg gctcttctga tatagtctgg gtgggctggg tgattttctg 109740ttctccattg aacttgcatt cctctgtgga ggtgttggct gtctgtcagc tgaagtagga 109800tggctttagc tcagggggct actcaacatt tgctgtcatc ttcctcctgg taccattagg 109860ctaacctggg catgtccttt taatgacatt ggtggaggac aagggcacaa gcagaaacat 109920acaagtgttc ttttgagcct ctgtttctgt cacatttacc aacattccgt tggccaaaac 109980aatttcacat gggtgagttg agagttagag tggggcactg tgaggactca aggcaaaggg 110040catggctaca gggaggggtg aaggattggg acaatttttg caatatatta tatagcccat 110100actgctaatc aaatcaaagt ttatagatct aacggaagcg tacgatttct gatttccttg 110160atgaaacact tatcttttca gctttactga tgcccagtct ctttttgata ggctggggga 110220aagtcagaga aaccagaaat gggaaaaggt tgaagtgaga tcttgaaagg gataggtaat 110280tattaacaat tagctattgc atgcattgta taatttcaga tgtacccggc tcatctcctt 110340ttataggaca ctgaagccca aagcagcaca gtgtatcacc cacattcccc cagccatgtt 110400aatgacacaa ccaaaagttg caactcagtc attctaagcc cagagccatt ctccacaata 110460tcttttaaga tacattctcc ctaaactgcc tcatatgcct ccctgacccc gtcactcctc 110520acacaaacaa tcacttttgt aaatggttta tgtttgaaaa ttgccttgtt atcacaattc 110580accatgaaga gtgggttctt attttgagtt ttccttttta agtttacaca tcatacctta 110640aagcaaaata atccccttat gtgcatgcat gtgagtacgt gtgcatgtgc acacacatga 110700gcatacacac gcatgcctac acacactcat tcacacacac acaccacaca cacatccctg 110760ttttgccctg cctttacctg aatatttcca atcagaaagg gacattttga atatagtggt 110820tatattaaat cagcttgagt ttggtaggtg aggctttgaa ttatctttgc tagacctcta 110880agggggagct gccagagagg tcactgtgac actctcagtc tgagacaatg aagcctgggg 110940aataaattta gttagaaatg agattaaagc tttagggagg tattgtttct taccagcatc 111000atttttcccc tttcttgaag tagtcaaagt gattgctcaa gactcaggtc aggacacagg 111060gacatggaag gaaatattct caacatttca acagtggcag tttttaattg atgaaattat 111120ggacagatgc tattctaatt gagaagtact ttttctaaca aatagagttt ctatactggc 111180aggtagatat cagatcaata agtctgtgat aagcatacca tttaaaagta tgctatattg 111240aaaggaagct tatatgtaga ctgacataaa taagagtttt agacctgaag aaacaagaaa 111300atcccaggta gttttatacc tgtgtttttt tcatatttac tcattcttca tactctatac 111360atcagtgatt atttttcttt ttttgagcta ttttgcatgt taacatagtt gccaaacttt 111420
gactctgagg agagcagtat tgctgtctcc aaaatattta tatttgaggc ctatctttta 111480gacagttaca ttctaaaata tgtgtggtcc tgcatttttc acttaaaata tctcttattc 111540tgctctgtgc aaggtcattg aggattaaga caaagttttc tctaccacca tcaaccttca 111600ttctttttgg tgggtagatt ttatattttg tttctctaaa caagagaaaa cagttatagt 111660tccacctctt gtgtgcttcc tctcctatga gttgtaggct cagtatgtgc acatatgcaa 111720gatttcaagt gcaaaagaga acagagtcgg aaacagattt tcacagttcc cgtctgggtc 111780cttgtgtaag tgccaatgta aagctattcg gtgattagtg aaaaacagta taatgttgcc 111840tggctctaag gaaacagctt tgaaaatcta aacttaataa gcagatcaat taggtggggg 111900tttgggatta ttgtatcaca ttccaatggc atgcagtgtt gtcaaaaact tttttctcca 111960gaataaaaat tacaggaggc atagagttac ctttcaaaat gtcaggaaag agggttgggg 112020aaattagtta ggaataatga aaatatatca cgtatatctc aggaagcata tactgtgagc 112080atctgggtat ctcagggtaa ggggtaaggg gtaggggagg aggaggttta ccctgcatcc 112140atggaggtaa atggattaag ctgaatgtct gggaagacga ggattgctgg gtggaggtgt 112200gaggcccagg gctcggggat accatcagat tacttgctcc tcaagttttg ggagctaata 112260atacctgtgc ttgcatttcc ctgcaaagca agggatacct tctgggataa gtcccgaggg 112320aatcactcat aactatttct gcttccctcc cacttattta acataaatat agggcctagt 112380ttggggttaa attcagattt tacacattta tgtctctgtc acatttcttg ccaccaactt 112440actgaagtct gaaatttctt taacaagagg aagtattcca tcccatagtt agaaatgtaa 112500gaatttcaat acctgtttaa cacagcgaat gagattaggc taaacttaac agtaattgga 112560aacgtattct gtacctgcta ataactagaa aaaaaacaaa gacaaacaag taagcctttc 112620tgttttctct tgagacgtgg gaattgtaca gtaattacag tgggatgtaa gtttcacgaa 112680ggtagagatc accctgtgtc tttctcagtt atattccgtg catttgtcac agtgcctgat 112740tcataacaaa ggattttcat ttttgtaagt agatggacac atggatgatt ctagtatcat 112800aaagactatt taaaaaggtg tatacaagat aaaatgttga cccagaggaa gcagtgaata 112860attttgccct ggaaggatgc agataaaggg agactttctg catccctcca ggagatgcag 112920aactagacat ttgaactcga tcatgagaga tgaagagagg tttctcagaa cttccaggat 112980ggtatccagc cagaagaata agtgtgtgta aaggcaaggt gggaaatgac atgaatgttc 113040agggagctac aaataatttc acaattctag gtagtatgaa ccatggcaga cgaggatcat 113100atcatgaatg gcctttttgt gtaagtgaca tctcagattt gagtcaagac agctgctgct 113160gggaactggg gagtggacat agaaaaacaa ggaccatagg aacttgtttc cacctcacat 113220tttaaactga agttaagctt gcttacctgc agtcatattc agatttagat gctaattagg 113280gaacatttgt tagtatggaa gctacagagc ttgaagaact ataacctata ctgctccaat 113340gagattttat attttaaaca catattaaaa gaaaatttta tctttttaaa gtcaccttag 113400agtcagatga atgtataact gttttctaaa ggaactatca tttcataaat ggaatttctg 113460cctggttcct ttaaatagtg gcttcattta ggatttaaaa tatttcattt acacatggct 113520ttccagaggc ttcaaggcac acctggatgg gtgagcgttt ctctaagcct atggtaagag 113580caagcatgac ttggatttct tgtactacca gatatgtgcc tttgtggggc agggtgtttc 113640cttgttagat atataaatca accagaaaga ttgggtcgga tgggtactgt gttattttct 113700taatttaagg agaagatcta taatggaaat tttatttagt ttctttggac tgttttcatg 113760catttagttt tataaaatgt attccaattt taagcaagga accctacatt aataaaaatt 113820gatttcttca gcttaacttc aaatatgatt ttaagcaacc ttaggatcat gctgagttga 113880aactatggaa ccgctaccct tcccacttat agctcaacta ctgctattgt cattgttttt 113940ttcttctact ttcattctat taattgttag ataaaataac tccttatttt gcatgttgag 114000gtttaacata agttctccac ttgatagagc atttaatttt ggggggacgg ttcttagccg 114060attatattag ttcagtggtt ttagaataag acgtttgtgg ggaggggcag cagaatcttc 114120agtcattgat tctctcttta taactttgcc cttttagctg gaacagatta cttctgtagt 114180gctgctagcc ctgcacagac tgaatcacaa ggaagaaact ttgcataaac catttatact 114240aaatgtttgg tatgctggca acataaatca acaacaatat ccattacaaa ttcatccctt 114300tatcagtatt atcttgatag aactgatccc aatgtgtggg aatttgaacc ctgcttcaat 114360acatctagcc ctggataatt aattattcag ggcacagaga acctggcccc tgcaatgtag 114420agtaattctt cctggtcagc gttaactcca ctggcaataa taaatagaaa aagtctctag 114480tgatgccagt attttaaagt gcttaatcat ctttcccccc ttctcctccc ccttagtttt 114540ctttaactag gatttttcag caataaatct ttaacaatac aggtaggaat tgttctgtaa 114600ttaaattttg ccctgcttaa tttcttacct tttatgtgtg attaaaaaaa agttacattt 114660taagaagccc tgatcaaaat tcaaaccaag aaaaatcctt ttacagaata ctgagaaatg 114720ctctatggaa gataaagctc ttgattttga atattagata gagcacatac acataaaaat 114780actcataaaa attgttcatc aacaccagtt ggggtgtcaa atattctaaa gttttgaatt 114840gcttcacttt catgcttacg atttgtctgt attaggaaaa gcctgacata tacatatgtt 114900ccaacactga gagaataatt acttatttct taactttcag ataattgatg tcagctctgt 114960aggaaaggga tattttcact atttttatgg tacacgaaat gcacttgctg aagcggcagg 115020tagatgcctg gctgagaatg accgtgtatt ttaaattgtg ccctgtcaag ctgcccagcc 115080atcatgcata tcctgggtga cagggacaga tgtgacatat ctgttgtgac acacttttgt 115140accaccagtc tgtggctcga ggtgactcct ctccctcccc tgatgctttc cccgctgcag 115200gacaacataa cgatgcacgg atgaaccttg ccattgctct gactgctgcc aggtatgggg 115260ctgccacagc caattacatg gaggtagtga gcttgctcaa gaagacagac ccccagacag 115320ggaaagtgcg tgtgagcggc gcacggtgca aggatgtcct cacaggtatg ccaggtatct 115380gggagggagg tatttcttag tatcttgcct agcttatata atgactgaat tataattttt 115440ctgtcacttc tctttcattt atgccacgct tcagagtcat attaattata ttcgttattg 115500tcagcaatga ctggtttaac gtgaaaattt cccttgtagg acttggcaat ttttcccctt 115560tgtattatag ttgtaataat gataatgata ataataaaat gcccaccaaa ttcccacagc 115620tatgaatgga ttcctttttc tttacaaaaa catgttatta ataaaattaa ataaatgtgc 115680caagtttcaa agtatcattt gggtgacaca ggcattgtta cacgttgtta aagtgaatct 115740ttaactttat ttagattttc attattgccc tggctctagc gccatataag taggcagttt 115800ccaaattata tcgtttcttg aacaaaagct ctatgatata cattttctca cagcagtgct 115860gctcacacat ttgagttttc aggagaaagc ggcttattta ctcattctac tttttcatgt 115920ttggtgcttt gacgctgggc acttataaga tcgacagaat ccataatgag aaagtggaat 115980gcttttcttt cttcaattcc gacttgtttt tggtaaattc actcagatga gagccttttg 116040tttggctctt caataacttg agctgctcag gtcacttagt gtggtatgca gtatacttca 116100ccatgggtta agttaataga tgaattagta atacacagca tccgttaaca gagaaacaaa 116160tgaggtgtgt gattgatgcc actttctttc acagggcagg aatttgacgt gagagccaaa 116220tgtgttatca atgccacggg acctttcacg gactctgtgc gcaaaatgga tgataaagac 116280gcagcagcta tctgccagcc aagtgctggt gtccatattg tgatgcctgg ttattacagg 116340taattgtctt ccaatgtggc agttgtcacc caaaaaagag ggtcagcaga gattgtctgg 116400tttatttctt cttctagcat agcaatagat ggtctaactt gctgttcaaa atcaggagaa 116460ataagtaaag gaactcttcc aaaacacaaa atacaaacca gacttgggaa gttttagcca 116520cttatggcaa tactcgtttc taagggttaa tgctttggga tctatttttc tttgttcagt 116580gtagtcacct tcagagacta agcattggta tttttgaatt aaacttttac ttaagtcatt 116640agcaaaatta attttgcaaa attttttata aaggacgtat tttagttaaa acaataagac 116700atgaattttg cagaataccc aaatttgctt atgaagaatc tgaagatgtg ggctacccaa 116760gtattcttag gtttataact tttgaaatag attaaaaaaa atattgtagc aagcaaaaac 116820
cagatatatc taatctatca aattaacaaa gataattgag gatgaagaaa tgggcaggag 116880tagagataaa ataagaatag ctattaaatg aaaatcattt aagctgggtg atgggtacat 116940ggagcgtcat taaactcttt ttatatatgt ttgaaatttt taataaatta caaaagaaac 117000atttgtgtgg ttctggctaa aaaataatta cccataacgt acattttaag gtatttatag 117060aagaaattcg tatggccatg ctaagggtgt agagcatgtg tactgttttt atttccatat 117120gtggtaaacc tagtaggcaa agtagtaatt ctatatgaaa taatgactta tgtgcaacag 117180tattcattat ggcattgttt gtagttgaga aatattagaa gctacttcag ttcctcacct 117240tagggtaata gataaataaa ttatggcata accacacaag ggcatattat atagtcatta 117300agaattataa ttataaatac ttttaatgcc ctaggaaaac actaatgata taatgtcaat 117360ttagagtata ggacttgata ttttatgtgc tgtaggttct aacaatgtat atagaaaaag 117420actggaagga aatatgttaa atgtgaatgg tgattatttc taggtaatat gattatgggt 117480gagagttatt tctttccatg ttttaataat tttccataaa gggaatatac agcttttaat 117540atttgataaa gtggatgtgt agaacctatg atattttgtt taatacttcc attctcctag 117600aatgctatcc tgctatgcta tagatcatgc tgccttttta gttgttggtt gtccggtaac 117660taaatcttcc tgaagagtct aagatgcacc taattggtac cagatacact tctgagaaaa 117720tcattaaaga gaatttctta tccaccaact tccaaggttt cctttgcgtt ttcaaaatgc 117780gtatgtgaga gaaataatag tctttaggat ttttactttg gactggagac atcaaggtgc 117840ctcttaaggc accactgcat aagataatag aaatgtatgc agtggtacct aattgagttt 117900ttaaaactgt tgaccagaag cttagttcaa atgaaaagga gagaaataaa tatatgaaaa 117960tgtcccatat gtttctcgta tatagagaag ttttcaacat tagttccaga tgcaattgta 118020tacgcttttg tgttcgatta aaaatatcta aggccagcta gtttatgagt ttttgtttgt 118080ttgtttttaa gtatctctaa gaatggagtg tagatgtaat catcatacat cttatatttg 118140tttagctttt actagattca acatattctc atgtgttgtg tgccttatgt tttcttcact 118200atcatcccat gatgtcagaa gacagagatt attccccaca gaccttatgt taattctgtc 118260attaaataat taaataattt gggacaagcc attttatttt gtgaacctta gttttccgtt 118320cattaaaatg ggcaaacatt ctgctccttc agagtttaga gacaaagaga ttgttcgcag 118380aattaaagtg ataccttgct ataggacttc tggggtttgt tcatcacttt tccttgggct 118440gtgtggcctg ggcaagctac ttctttcagc ttctggttcc ttatatcttt tttttttttt 118500tttttttttt gagatgaggt tttgctgtgt cacctaggct ggagtgcagt ggtgccatct 118560tggcccactg caacctccac ctcctgagtt caagtgattc tcctgcctca gcctcccgag 118620tagctcgccc gccaccacac ccagctaatt ttcatatttt tagtagagac agggtttcac 118680catgttggcc aggctggtat caaactcctg gcctcaagtg atctgcccgc ctcgcctccc 118740aaagtgctgg gattacagac gtgagccact gtgcccggcc ccttatgtac tgataatacc 118800taactggtat aaggtttgac aatgatcctt ggattatttt ttctctgttt tatagagtgc 118860tgtatcccca gctattggta cggtgtctgg cacgtattag tgcccagtaa atattttttg 118920aatgaatcct cctaaatttt ttatgttaac ttgcttatat tgagattgtt cctttattta 118980tattaaaact ttaatgtaac atttatgaga ttttttaata tagagaattc aaactatgaa 119040aataaaacat gattcttcaa cttactgccc agctaacccc tgtagacaaa aataaataaa 119100taatgtaagt acataactag aaatttcaat catcatagaa aaaaatagaa atatgaaaat 119160gaaaattgaa agcctgactc ttattccccc taccttttgt tccctccccc agttcttctc 119220tggtaattaa taacctcttc ttccaattcc ttccttaaaa tcatgttttt acaagcacaa 119280atgtgtattc tgttaaaaat tttgtgtgaa agtggtcatg ttgcatatat tgttctatat 119340tatgctttat acacttagta acagagtaga tttggtgtct tttaagaata aaggtatata 119400ttacccagaa gtaatatttt aaaaattctg aatcctaaaa attcagcatt agctttaggt 119460ctcctgaagc atgaaggtgt tagttacagc taggtctgtc tcaggctaag atgcagtaag 119520tgacctcatt ctatgttggg agaaattaat atccccattt ttggcactgt gagctataac 119580cactacacat cctgttggaa gctttgaagg aatggtctgc agcctagttc ccattcaggc 119640tctcttgcag atgagcccct tctctttgga ttgtgaccta gattaagggt tcattccctc 119700attcaggact gaccttgagg caactgtact tctggcatcc cataggggtg gttttcctct 119760tcctatcaag tcattctctt gggtgatatt aggagatccc aaactgaaat gactctgtgg 119820tatcatcagt caatcatact tagcagctgt cactcaacag ttttgactag gtcttcaggg 119880atgaaccaaa gcttctgggg ctgccccaca actggctaat gataatttaa aaccaggcat 119940gtttcagtta gaacctgatc catttggaaa tgcagtgagt tcagcaagca ttgatttatt 120000gtgtgtaggc tggtaaagga agagtaagga accatatgtg cagtgtatct tcttgggctg 120060ttttcagaag tatctttaca gcatttgtta attttgttgt aactttaact aatgttccag 120120ttgtggaatt atcttacacc atgatagcag ctttttaggg gaagagggga agaaggaaaa 120180attgttttaa tttcggaaaa cacaacacag aaattatttt taaagctgct ctttatatta 120240agaacaaatc tggaatttcc atgagagtta tgggtagatg ctcttccaaa agcaatgata 120300atacaccaaa cgactgaaat acatgaaaat aatcatctct gtttagccta ctggtgcttt 120360ttactactga caagaggtca atttttttcc tataatgtgt gtacatactg ttagctctaa 120420cactgattgc taagtagtta acaataaatt gatttcagtt taactaaggg cacttcagga 120480aagtgatggt ctgcatgaga aaaatattca ttacatttaa tcctagacac acacataaat 120540tagcagcaag cttttcctcc tgatgtcaaa tgaatcatat atattgaaaa aagacctatg 120600ccttgttgca ttgcgtatac tatcgctata gttgatatcc attctcaaac tttagtgtga 120660acttgcaaaa ttgatccttc atgagaaaac agacttttcc cgaagggaaa atatgaatta 120720ggtaatttag aactgatgaa agatatttcc aagtggattc ttaaattttt ataccattcg 120780ttaatagctt tgtcttccct ctgtgcttgt taattttatg tttgtcacat atgtcttcac 120840ttaagcagtt gattctctac aggttccagt tacatgtgtg ttggtaacca tcatgttcac 120900cttttaaact actttattgc attgcaagtt tttaaaagat tcagtagttg tgcatttcta 120960atggtatttc ctttttaggt tgatgaatat tataatttta tgattaaatt tatcatgttt 121020attattttag acacatcata tgatgtctag tccttgatat agtcatataa aacatgtcag 121080catataattc gtttatcaat tgttagaata tagaattttt ttgtttcaat atacttctgt 121140ttatcaaata gaatgaactc tgagtgttct aatctcattt ctaatctcaa agtgactgat 121200gtaatctaag gacatatcaa tccaggatca aaaaatatac ttagaaaaca tgtgactagt 121260ttcttctgag atttttagat agttttatga taaatacaaa gaaaaaataa tcatatttag 121320agaacaaaaa tacttgttat ttgtagtttt caatacatta gagtaaaagt aaaattattt 121380tctctaagta accctgagtg aaggtgggac tctgagctga ggtttaaatg attgcagact 121440tccagggtgc tttttggatt agctaagtag aagaacttct acctactctc tccgatgtgt 121500ctcagatagg ctgtgaacag tgacttcttt ctgctaaggc tgagtagaca tggcaagcgt 121560ttcatgcggc tgagggtttt ttgaagagaa agactcactc cttgtggttt aaataaagaa 121620gatacattag ttaagctgcc tatatgtttc tttttttata taaaatatta tcattatttt 121680ttacacagtt gttaccctct catttttaca cattgggata aatcattttt taaaacatgt 121740taagggaatg atgataaaga taaaaatctg actgtgtgtc tttttaaatt tttgtgtaag 121800aggagtttct tgaaatttgt tcaaaccaaa tagaattacg tttccatatt aaaaaataac 121860attaatagaa gctaaaaaca tatttataga aaatactagt aacactggct tgatacaaga 121920atccagtcaa atagaagcag ctatctgaag atttgtagtg ttctacttga ataggacact 121980ttaagattcc tctcactctt ttaacaacat ctccaaattt ctttgaatgg ctaaggaatg 122040aaataaaaca tcagctttta gtgatttaaa aacaaaatga tgggttttgt taatgtttgt 122100ttacagttgt ctcttctttc aaatatgttt tattatttga ttttagaata gtaaatatca 122160gtaaatattt ttgactatcg ttttgggaga ttttatgtag tctttcagta gaggcagcat 122220
tgtaccttga tttaacatag tacactacta gtgtctttta tgtaaaagat tttcattgct 122280aaagtgttcc tctcctgttt gaccttatcc taggacatta ctatttaggg agtagtaaaa 122340catggagctc ttttcttcta gatgtacatt ttgagatttt tgtaacctcg aaataaagta 122400ggaaatctca tatttggtga agtggcacaa gttcatctag gcataaagta gtaaagaact 122460ttgtgattac attttagttt ctagaaaggt agacccttca ctttcttatg tactcctgag 122520aatttctact gagcgatgaa atgtcaaatg tctcaggaaa atactttggc tattcttgaa 122580aaaaggctga agacatatcc ttaatttacc attataaagg aaagtataga catttatcct 122640gctgacatta tctgatgtga atggtccaag gagtatgaaa ctcaaacagt aggaagttga 122700agcccacttt cttaattagc cttgcattgt tgaagtgggg agggaagggg tagctctgct 122760gtggaatatt gttatcatca catttgtttc attctgtttt gttatctaag ttattttttt 122820cttttaggag tttctagatt agggtagggg aagtgcaagt aatagttcat atctttcttg 122880tttgcatatt tgagaattaa tgaataaata gtttttcgaa tgcagattaa tgttaaactt 122940ctgggatttt cagaaataaa taatccttct ttgtatctca gcccagagag catgggactt 123000cttgacccag cgaccagtga tgggcgagtt attttcttct taccctggca aaagatgacg 123060atcgctggca ctactgatac tccaactgat gttacacacc atccaattcc ttcagaagaa 123120gatatcaact tcattttgaa tgaagtgcgt aattacctga gttgtgatgt tgaaggtaac 123180taagcattcc tttaagtttg tctctctgtg tccatatctc ccaagccatt ccagctctca 123240ctggataaat gctatgtctc cagtctgact catcttcaca cttgtcctct tgtgcctctt 123300agaacattta tgagggccaa attttaacat aatggggaga aattgccttt tctcctctac 123360tttctatacc cacatgaaac ttatgcagtc ctcttctgat aattcctaat ggcaagatca 123420gagtagcccc tctagctctg aacactcatg tgcttccaga aaacgatgaa atgtgtacat 123480cacagtttaa tttctgcagc ttaggaatgt ctaatttaca gatgcaggat atcaatagga 123540aataattaag caaataaacg agaggctgga actgtatttg taagatgctt aatagtctgt 123600tactacatgg aaagtataat gttgaccagt gggcttagca aagaaggata agttacttat 123660tttaatggaa aaagccaaag tttacttcct tcctattctt actggggcat ttcaacttga 123720ctgtcagagc attttccttg gtgatttata tagttttttg tttttttcat tcaggccagt 123780ctcagaagtt ccccaacatt gtgcttctat atttcctctc tcagttgagt ctcaccatgc 123840atctagtcac tcaaattaga aatggtgtgc ttatttttgg tatcctttct ctttccatat 123900ccagtctaat gcctatctat gcttattctg aataccaggc aggtccatca gccacttcat 123960tgccctggtt cacatctgtg ccatttctca cttgaacagt tgcattggcc ccacaatggc 124020ctctttaggt caggtctcta tccctctcca gtctaatcct tctaccagaa ttttctttct 124080aaaacacaat tctgatcatc acttttctgc acaaatatac actttagata tcttctcatt 124140gactttagga aaaatgctaa attctcttgc atgaaacata aagctttttg ttttgttttg 124200ttttgtttga gatggagtct cactctgtca cccaagctgg agtgcaatgg tgtgatctca 124260gctcactgca atctctgcct cccaggttca agtgattctc ctgcctcagc ctcccaagta 124320gctgggatta caggtgccca ccaccacgcc cagctaactt tttttttttt tttttttttt 124380ttttttttta agtagagaca gtgtttcact atgttggcca ggttggtctc aaactcctga 124440cctcatgatc caccggcctc ggcctcccaa agtgctggga ttacaggagt gagccaccgt 124500gcctggccga aacataaggc tttttacaac ttgtctttgc atgtttatat tcaactactc 124560cccatccctg cattctgtgg tctaatcatg tcttattagg gtgaatattg ttttattagg 124620tttcatgtct tattatgttg aatcttcgtg tctaatttag gttgaagcct tgctctggga 124680cacccttttc cccaactctt catcttggca aaatttttat actggagaaa ccacctattc 124740tttgtattcc tgtgggatgt tggtcatatc gtttgtatat cacaacatta tattataggt 124800aatagtccaa gatgtattct cacttgaagc tccttgaggg taaagattct ttatttgtgt 124860ttagctacaa atcttgatta tcttatttgt atgctactca tgattgtttt gaattaaaat 124920aagttagtta tggattcatg gtttttgata tggtagccat ttgaaaatct atattaaagt 124980atacaagttt acgattaaaa ataatcttca tatatttata tcagaaatct tataatgttg 125040atgtcagtga aggacatgga aaagcactat gttagtctga atgcaactca ctagcatctg 125100tgtatccttt ttccccaaag gatttttttt tggttatgtg atcacatttg taaacaaaat 125160gcttcaaaac tgcttcaaaa atggttcagg tgactcctag tttaattgac taatccatat 125220tgtatagtat taattgcttt taatctaaag taatggatgt aatttcattc ttaagagtct 125280ctttggatat ctacaataat agtataaaag atactaaata catatcattg aaatccatgt 125340gtggttaagg tttatactca cacacataca cactcataca tggtatttga aaaatagttt 125400gacttgttta acatccatgt ttaaatactg aaactgcatt acaattttag cttggtactt 125460ttttttcatt ttgtgttcta ggaggagctt caacattgga ttatgattat attttaacat 125520attcattatt gttaatgaat cttccttcat ttgtaattta tctgtatata atatttgttg 125580aaagtgggtt tatatctggg cctgaagatc aggaagttta gattgttgga gcaagatata 125640aaattaattc aagagcagag aactcaatgt tgacagtttc caaaatctta aagttcaaat 125700gcagcgagga tgagaaatgt aactccttat cctgctaaga agctggatgt gctggaagca 125760ggtgtgggtt ccagaaggct gccgtgccca tattgtgggt ggggatctct acccgtgaag 125820tgagcgtggg gctttaacgg agactggatc acttatgttc tacctataaa tacatggaag 125880acttggcata cctggttggt ccataaaagt cttttcaatt aacataccat tttgtagtca 125940gcgaattggt gaaactaaca aatagttatt gttaattctg atgtttgata agagtgtttg 126000aaattaatat ctaaacattg caaaccaggt cttcctcctg gtgatctggg gacatcttga 126060gattaagagt cctccagggt aaaaagcaga aatgacagcc ctttcctaga ctttattgat 126120ttccctgtgt gcggcacctg tcacaacttt tctacttata aacttactct tcaaaaaata 126180gttaacagtg attctttata gcttcttttc attcttattt ctaattgact gaaatattca 126240tatttgagga attatgcttg atcttagtaa agagtatcag ttagctgaag tttgtctata 126300tcttaaaatg aggccagtca aatccatatt ttttctcaag gaattttggt gaaagccact 126360attctcttct aggctgatga aatgtgaaag agaagctcag ctttagccac cattcagtta 126420ttcaattaca tgctactgtt aaacatattt taatatttat agttatatgc ctcattttta 126480aaaacttcct attattgagc acttcttatg tgagacactg ctaagcaagc atattacctg 126540ttagatcttt gattcctcct taacaacctt atgagattta tttaataagt cccactttct 126600agttgaagaa actgaggccc agtgacatta agcttttttt tttttttttt ttttttttga 126660gatggattct cactttgtag ctcaggctgg tatgcagtgg tgtgatctcg gctcactgca 126720acttccgcct cctgggttga agcaattctc ctgcctcagc ctcctgagta gctgggatta 126780caggcacacg ccaccacacc cggctaattt ttgtattttt agtacagact gtgtttcacc 126840atgttggtca ggctggtctc gaactcctga cctcgtgatc cacccgcctt ggcctcccaa 126900agtgctggga ttacaggtgt gagccaccgc gcccagccta agctttttgc ctatagctag 126960ttagtattgg agccataatt tgagtataag taagtctggt tctaatactt gtgcttctaa 127020atattgtgca gtactctgat tgttcgttct gggtttcctg atgggatact atgtgcacta 127080gggagtggta agcttatcaa ttcatttcat ggaagctgct agataccttt agcatgacaa 127140tgacctttgc tctgcactga ccttatttga atttgaatgt taaactagag gaggtttcct 127200gtctcataac taatcccttg ggccagtctt ctttacagat ttaaaaaata aaatttcatt 127260tagatcctag agcaatagtt ttgaagtgtt ttacattcac cagggttgaa ataatcattc 127320taaaaggatt tattaaatgg gtaagtaaaa ttccaacaac agtttctgag cctccccaaa 127380tggctatatc cttaagaaaa aaaagtgtca aatcttaaca ctccatgcta ttcacagcag 127440ctgccacact tctgcaaaat gcgtcggaac aattctgaaa agcattttgc ctgttgggaa 127500taagaccatg tcttctttta caatttagaa agctttccat tcacaggctg aattttctag 127560attacttgtt tgttcttttc tgaaatgggt aattgatagt tctgcgtttc atattgagtt 127620
aggaagaaaa atgatctttt gaaaaatgta tcattaagtc tgctgtgtag aggttaatca 127680gaaggatgca gtgacaaaac taagctatga cttaacagga ttgagagaga cagaagattt 127740gctgagtgct ttatccatgt agtttttcct ggagtacttc tttaggcagc ttctctactt 127800gtacacaggt tttcacatag gcaattagta gtgtttatag attgcctgtt attcaattaa 127860caactgagct ggccatatca actcactgca gacctgcctt ttcattgcca gagattgtac 127920cagggttatc aacttacaca ggcttggatg tcttccagct gtttaattat agtgtcacac 127980gctttgcttt ttctagtttc attagtttat aaatgtaata tggtatctta tttctgtgat 128040taacacttca tttcagcagg ttaaacatgc tggtagagaa gtattgattt ctgacactat 128100ttcccctgtg ttcatttaga tattttcaca tgccttttat tgattgtttt ttgaaagtgg 128160caaacatgaa aaaaaaaccc ccaaaacata tgcctaaata actaatgtga agctaaaccg 128220cacatttacc tcttaattgt cacttcctgt gggctttatt ttctactaat ttttaaaaca 128280aacacttatc ttgccaagta tgagtaaaaa tatgaattat agtttgaaat aatcacatca 128340tacttaattt tttcaattag ccgtgaaaat tgtctgttca acttttctat ttatatttta 128400ccctttacat attacattac taaaatctga atgagttatt ttttgggaat gtaatacaaa 128460tgattcttaa acatgatggt aagagtattt tactttaata ttgccaagta cttttatagt 128520tgtcacttaa aaaattaact gggtgccctc taggtgagcc agtctagtaa gtggtcttta 128580atgatggatt tttctcaact ttatttaata gtagtttttt tctttcatct tgttttattt 128640aaagcattca tgtaatttgg aagggaatat ccagttgttc aatatatagt cactagaaac 128700ctacatgaag cctagcatat tatctcagag gcccaagcgt tcttgctttg gttttggtaa 128760taccagctct tatagtttga tataaaaagt ttagagaatg ctttaatatt ccacttaata 128820tttactgata actgtatatg accctttact agatacagta tttggggtat tgaaaaatat 128880tataattgac actgctggag actttcagca tggtgagaaa gagatgaagc aataatgaaa 128940ttttgttaag ctatcctggc agtatgagaa ctagaaaaaa tttgagctgt aaagcataga 129000ggccatgagt tatgttattt tcttcacatg ttataccatg gacagttaaa gtgggtgatg 129060gtcatttatt aatcttgact gtaacctgta tggtaaaact ggtttctggg tgagaacttg 129120agggtggaag tacttaattg aatgaaaata tgtttcattt gtttgaattt atgaaattag 129180caagagagga ggaaagcttg aaatggtaca tattgacatg agtgacaata aagtgtttag 129240gagctattac agcagatttt attttattct catgagcaaa ggaagtgcag tccaatttca 129300gtgaaaggat gctccgttgg agatgtaggc aaggtggcca gtagaaataa ctagcttaat 129360ctaccagaca agtctgagat ctactgcttg gaggcttttt actgtagagc tgctctgtgc 129420tatatgatag ccatcagcca cttgtagtta tttaaattta aattagttac aattaactga 129480aatttgaaat tcagttgctc agtcaggtga accacatttc aagcgcttaa taaccacatg 129540ctactaatgg ctagcgaatt ggatcgtgga gctatgtaca gaatgtctcc atctctgcag 129600taaagtccat gggacagtgc tgatcttgag aatctagagg tgtttaaata tacacttaga 129660gaaagacata ctaaatttaa tagaatcgtt gaatttgggt ttttttgttt gttaagtaaa 129720atagagaaat tcattatctt gtaaaacaag aactccagac acaacaagcc tcaagaggtg 129780attaatgatt tgtcagggtc taagatatat tactcttatt tatcttcact ctgccatcct 129840ccaaggtggt ttcatcctaa ggttgtttcc cctgtggtta cctcatggct gcaacagcag 129900catgcagaat ttatagatat cccaatgata ctttattcag actagcatct actaccttga 129960atctttcttg aaaaaacttt cgttagggtt taacaatgta caaaatttcc actgtgggta 130020aatacgtgat atggagagga acctgttagc ttcgtcagtt gctgtctttt attcaagaag 130080ttggaattaa tgtctcacat gtttagctgt gctgtgcccc ttagacatgg attataccca 130140aggtcctgag gagtcattac tgaatgagtg gtgaggaggt ataagctctt aatacttcag 130200tggcaaatag agttttgaat catccataaa aaccattaca tctcaaggtt gggaggggaa 130260ctgttgtaga taacctgatc caaccacctt ctggtctaat actgtacaaa tcctgtaggc 130320cataaagact aattcatatt ttaaataagg aaatttgatc atccttaaat gtattatttt 130380taataaattt attcccaaca ttcaactgat ataattctca ggctaaaaag ctgaatccta 130440agatgtcaag caaagtaaat taaagttctt aacacattat gaaccctttg tgactcggaa 130500tcttttctta actctttttt atttttgact atcaactcca ggagtattaa aaatggtatc 130560aatatagata atgttttaca tagtgttcct ggatgaatga aaaacttgaa tgtttcagtt 130620tcagacaata caatttcata gatgtgcttt tctgcctgtt ataacagaaa aggtaaggca 130680tttgccacag attatttgca tatgcctcaa atttactcgc aaagcctaat tttccatcaa 130740ataactttaa aattagtcag agagcttcaa gagttggaac caaattcctg atcagtgtgg 130800ctacctaagg aatttcacag acactagagg gactgtgggg tgaccacaat gacgttgtaa 130860gcgggtttgc taacttttat ttttatctca gagggtgatg tgaactctaa tggaaaatag 130920tggtaatttc ctagttaggt ttttggtaac gattaaaaca ctttttacaa cttgttttga 130980ggttttatat gactggtaaa attgtgtaaa gcaggaatta atccccattc cacagaagcc 131040atccagatta aatctattta agagaatgca cacatcagag ggggcagagc tagagatgga 131100atcccagcat tgagatcctg tattgtctcc agttagtaag actgagaagg cttaggcttt 131160cctcgccagt taattatgtt ggtatttcag ttacagtgtt tggattatgg gtttgattaa 131220acatcctgta gaggtgatgg gtatctactc tttcagatta tagttatgtc aagagtgtag 131280cctttaagaa tctatagtaa aatatgccag attaacttga gttgaattta tttttagcag 131340aaacctatta ttcattttag atcaagatgg agaaattatc tgtcatccaa catagcttgt 131400tttgcttata cttggtaagg catggttttg ttttattttg ttttgcttca gtaagaactg 131460ttccaatcat ttcaaggagt ctttgtgact ttttacacaa agagtctgct agatatatac 131520ctaactactt ttcaagtggt gttgtatata aatatacagg atgtttactg tacaaaggca 131580catgaccaag ggttgagtgg aaactgaaat ccagccctgc cctctgctca caaaactgtg 131640atccctgaat gggatttatg gatttgaact aaaacatgga catggtatgg gctaataaag 131700gtcctgatac ttccatattc tatgtaaaat atttccttaa ccctttcgta atttgagact 131760tgaattatat cagtttatcc ttttatcttg atttttagat tatggttaaa tatgcagaac 131820ataaaattta ccattttaac catttttaag tgtacagttc tgtggcatta cgtacattca 131880cattgtcatg caaccatcac caccttccat ctccagaaca tttttgtctt cctcaactga 131940aattctgtaa ccattcaata gtaactctta ttccccctct cccaacccct tggcaactac 132000cattctactt tctgctccta tgagtttgag tacgccaagt acctcataga agtgcaattg 132060tacaatattt gtcctcttgt gactggatta tttatttagc acaatgtctt caaggttcat 132120ccatgttgca tgtgtcagaa ttttcttcct ttttaaggct agataatagt tcgttgtaag 132180tgtatgttac attttgttta ttcatctgtt gatggacact tggtttgctt ctaccttttg 132240gctattgtta gtaatgctat gaacaaggtt gtaaaacttt tctctgctcc atttctttgg 132300agtatatacc cagaagtgta attgctagat cttatggtaa ttctaaatgt ttaatttttg 132360aggaatcgcc atacattttt gatagtagct gcaccatttt accttctaac cagcagtgta 132420cagggggttc agtttctcca catcttcacc agtatttgtt attttctgtt tcgttttgtt 132480tttgtttgat aatatccatt ttaatgggtg tgaagtggta tctcattgtg gttttcattt 132540gcatttcccg aatcattagt gatgtttact atcttttaat gtgcttattg agcatttgta 132600tatctttttt ggagaaatat ttattgaagt ctttcatttt ttaattcaag ttgtttgctt 132660tttgttgttg cattttggga atttctcata tgttctgcat atatgatata ttcttatcag 132720tatatgattt accggtattt tctctattct atgaggtacc ttttcattct gttaatagtg 132780tctttttaat gttgatgaag tgtgttttat ctattttttt cttttgttgc ctgtgctttt 132840gatgtcacat ccaagaaact attgacaaat ctagtgtcat aaagctcttc ttatatgttt 132900tcttctgagt tttatagttt tagctcttat gtttaggcct ttgatgcatt atgagtaaat 132960ttttgtatac gatataagat aagggtccac cttcattctt tcacatgtgg agatccagtt 133020
ttcccattat cttgaagttt tcaagactat atacagtcaa ttttttatca gtatgggtaa 133080ttgctgggag tatgatgaag aaggagggca cacatgaccc caagtcacag ttgaagcaaa 133140attatactca tgtttctgtt atccagcgat ggattagctg atttaccaca ataaatgcag 133200caggaccagg taggcattat tttcccagta gtctatatca tcaggcaact ttcagtctgg 133260tgcccatagt cagctcccat agctaagcca ttcctgaagc tggtctacat gaagaagggc 133320agattccgaa aattctgccc tagggtcttg cccttgtacc tgatagatga gaaccatagc 133380tcaccttctc tcttaagtca tttttagatt agtaatgagt gctgttcaac agctgtctaa 133440ttctaatgaa aacttaatca gcctggatat agttgggcat tgtattttga cagagatagt 133500aaagatattt tcatggcatc ttgagtattg aaagaacttc catataatat aattcaagta 133560taatgtacct actgggagaa gccagtatac ctgtttctgt gtcagtgcta aaaataatgg 133620aatgagaaaa gaaaaaaggc agagtatgga agttgtcaga agttgagaga cagtacaatt 133680taagaggtaa gaatatggac cttaaactca gaaggaagaa ggttctgatg ctgttttacc 133740atatataaga tatttgcctt ggccaggttt gttgcccagc ttggtttgtt aaatgtttgc 133800attcatctgt tttctgatct gtatggtgag aataataata accaatgcta tagggctttg 133860tgagattaaa tgagatgtct atgtcaagtg tttgcacagt ggtaggcctg ctgtaaacac 133920tcaggaagag ggtggtattg atgaggggag aaaatataat atcctgttac aggtttgttt 133980aatttaataa ggatctttgt cttccctgag aagagatagg aagatgtatt ctagaaggaa 134040atttaacaat tcatgcatcg ttagcattgg ttttgattgt ttctgctttt ttttctttct 134100tgtttttcta actgctccag gttgcttcag ctggaaggtt gctaacattt gcctcttttg 134160cttcctctgt ggtatcaatg tcagtgattt ccaaagttct ttactttttc atggaaatta 134220tctttgtaac agaataattc tttatgagtt tttcttgtac ctatccacaa acttagttct 134280catctttagg ctttctctcc tttttaaagt gcacacatgt gtattcctgt caccgaactc 134340tttttattaa ctgtttaata tttttatcaa aatatttttg agcaatgttc ataacccttg 134400gcattgattc ctaccagtga gaagagggga tgtcctggca gcatggagtg gaatccgtcc 134460tcttgttaca gaccccaaat ctgcagatac tcagtctatc tcccgaaatc atgttgttga 134520tatcagtgag agtggcctta ttactatagc aggtatgaga tactaccttg ttattttctt 134580ttcttttttt tttttttttg ttatagggac agggtctcac catgttgccc aggagggtct 134640tgaactcctg gactaaggtg attctcccac ctcagcctcc caaagtgttg ggattacagg 134700catgagccac tacacctggc cttctgtctt gtaatttgga tgtgctttag atataagttt 134760gacttaaatt gtcctgccag aatctctttt aactttattg aacaagcatt ttttttattc 134820tgaattaagt tttacacaat cccatgtttg ttacaaacaa caggaaaatt ttgttattgg 134880taagttgata aatggttaat tataagaaaa ataaggggtg gcaacctgat gaattgtcta 134940tcaatttctt tataggtgga aagtggacaa cttatcggtc tatggcagaa gataccataa 135000atgctgctgt caaaactcat aatttaaaag caggaccaag tagaacagtt gggcttttcc 135060ttcaaggggg taaagattgg agccccacac tctacattag gcttgtgcag gattatggac 135120ttgaaagcga ggtgagtgtg cattgccaca tcatcttact ctttacctaa tctctcactg 135180ctgccagtga cagaactggc agcaatcagg tctccctgat tgagatttcc ttttgctgaa 135240tacatttctg ccagtctgtt atggaaggaa aaaaagatga tcttgctttg ctttacttgc 135300ccaacaagca atgtacactt tatgaaatgt attttattaa aaagtggagt ttatttggtt 135360tgtttctagt gtatagggca tcccaaattc tgtctcaggc atgaagaagg ctgttcttca 135420gaagtggtat caggagcaaa atgaagctga agcttcatca gtcattgatg cagtgataca 135480tgtgtaagag agttagtgct ggcctaagca ttcgaagccc aagtggtttc cttccacaaa 135540atcaaaatat aatactctcc gagagctatt agttacaggc tgtaagaaaa ctgacaagat 135600aagcatgtgt gctttttgct ttgtgcctag aagctattga tattcaaagt gcctgcctaa 135660cgcagcttta tttctctagg tggcacagca tcttgccgcc acctatggtg ataaggcctt 135720tgaggtggcc aaaatggcaa gtgtgactgg caaaaggtgg cctattgttg gagtacgtct 135780tgtgtcagaa tttccatata ttgaagcaga ggtatgtgaa tggtcacata ggaatttcat 135840gtctgccatg ggataccatt tatctgttca tttgtcatct ttaaaaatta tatgtagcta 135900agttttaatg cacatatgtc agctaaaacc aggactacta tgtctttcca atatagatcc 135960ttttatttat aaaattttaa tcttaattct gaagtgatat tgatagtggt aataataaca 136020acataaaata taggtttctt ttttctccat caaactgtcc ctaaacgtgt taattcacag 136080ttgtcccttt tgtgcatttg cagctcttta agtttgtacc actcatgtcc tacctacata 136140tcttgtgaca gtttctcaaa ggtttaggtc ttgtttccca tcaagctctc cagttttgtg 136200aaatcagaga ccaggctttg tatttatttg taattcactg tgccttaaaa tatcgatgtc 136260cagtgaacat ttacttgttg attgaattca ggattcctat attctggtta ctctaagaca 136320aaaaactata cagactaaag aggtgagatc ataacttttt gacactcgtc cttttcctag 136380tcagctatgt aaattaaaat ccacattatg tataagcata gagaggtaag tgatgactgt 136440ggatggttaa tgagaagtga tgcaatttgc atgtttatgc caggttctat tcaggaaaaa 136500ggcattaact cttgtccaat ttttgccatt ttccaaacct cctttggcag gcagatggaa 136560aacttgtgtg atgccactat ttgtctcatg cctgctggag gaccagagag tataaagaaa 136620aatattacct ttgtgaagca cacatttatt ttttttaagt gaagctttat ttctcatttt 136680ctaaagaaga gtctcaacta ataatttttc tttaattcaa actcaggtga aatatgggat 136740taaggagtat gcctgcactg ctgtggatat gatttcacgt cgtactcgcc tggcctttct 136800aaatgtccag gcagcagagg aagccctacc caggattgtt gaactgatgg gcagggaact 136860gaattgggat gattataaga agcaggtatt atatagaagt ctttaaaacc acaattccta 136920ttgtaaacgc ggaatggctt aatttgttag tagaagctag cgtagttttt gatgataacc 136980ttagatttct taccagtttt tctttgtgtg tgtttaatat tgttaggcct taggaaaatg 137040gaaagatatt ccatttaaat ttagttgttt atggaaaatc tcttgtagaa aatgctcata 137100aatttgtggt cataattcct gaaaaagaaa gataataatg attgtagtat ggaacctttt 137160ctggttttca ttgaaaacac tcttggaaca taatcagtga aatgcaaact cctgctagtg 137220tgcctttggt aagaaatgtg gtttttctgc ctgagcacta aatgcttatc atttgtgtca 137280tttgagattt tatatttata ttccaaattt taaaagctgg tgatgctcag atgcatacac 137340acacacacgc acaacacata catacataca tatacacgca cacaaacaca catacagggt 137400taggctaagt agatctttat gttttttaat taacccaaac tcttgtgata tgcagagggt 137460aatgaagcat ttttatatat tcataaaata ctttctgtat tcattgggtt ctgttccttt 137520ctcccaggat ctcttgaacc catttcattt aggcactttt gccaccactt tgctgcatta 137580gctctggtca atctcaccag taacctccat gttactcaat aaatttgtta acttgcagtc 137640cttgttttac ttggccaatt agcagcattt gacacaattg attccccctt ccttcttgaa 137700caatattttg ctttctggaa caccacctct cttggatctc cttttacctt cccaaaacta 137760ctactcctat agacttttcc atgtcataaa tagctatcct tcagttactc aagcctaaac 137820attagtatca tactttatgc attctcttat accctatcta aatccatcag caaatcatac 137880catatttatg tttaaataac tgaattcaac cctttcattt aaaaaattat tttattttta 137940attttttttt aacaaacact tgtgttgaag tgaattcaac cgtttcttaa cacatccaac 138000ttccatcacc ctgattcagg tcaccggtat ctgtcacatg gattaccgca atagcttccc 138060tccccttaac tggtgtccct tctttaacaa cttcagagtt cagtttatta atacaaatcc 138120agatcattgg atatgtctgt gtgaaattca aagggaaggc ttgggctaga gatataaatt 138180tgagttgata tttaagccat cagactggat gagattatat agggagttaa tgtagtcaga 138240tgatgaaaac agtccaaagg ccaagcactg gagcacacca aagtttagaa gcataggata 138300taaggaagaa tccaggaaaa agagaaaata atagctagtg aggctagagg aggtgtaaga 138360agatgcaaga gggagttttg acctaggggg taagacagta tatcttagtt catttgggct 138420
gctataacag aaataccata gactgggtgg cttaaacaac aaacatttat ttctcatggt 138480tctggaggct gggaagtcca agatcaaggc atcagtagat ttgggatctg gtgggggcct 138540gtgtcctagt ttatagacaa acattgtatt gctgtgttct cacatagcat gagtgaatga 138600acattattgg gcatctatga tacatgctga cagtgcaagg aaagaattag tcacatggcc 138660tgtttttaaa gtattctcat tctggtatag atttccaaag atgattacat atcagtcacc 138720tgggaaatgt gggaggaaag ggtttgattt taggagttcc ttaatcctga tcatgcctct 138780gacagggata aaatttaccc ccatgttata gctgagaaaa caggttcaca gagtaggact 138840cttttcagag tttatatcaa cagtcaattg taaagcaggg attgaaacca ggtattctga 138900tcctagagca taagctttct ccatcctgtc ttgctgtgtc tgcattagct gtactggttt 138960atagctgttg aagaagtctt gagctttagt ttgattagct aaagctccgt acagaggcag 139020cacaggaatg aaacttactc ctgtgcttct gtcagtcctt tttaccatat agaaggattg 139080gggataatgt gtccttccaa aaattatggt cggaatgatt tgaatatgca ggaacatatt 139140ttatgctagg aattcaggct gtaggaggta ggaaggagga agaaaattct gtaaactggt 139200tgtgtttggc cctactaagg aagctagatt taacttcagt tcatatttta tgaacagatt 139260tatgtgctca gaaatgctct cccatccccc ttaacaaagt aacataaata aagacctaaa 139320gctattactc agtatctgag atgtaggagt attaatgaga gctgtggaca gtctagagag 139380aacaaaggag tggggagaaa gaattaaaag gttagaaagt tggacttctg agaaaaggtt 139440aaaagcattg ggattattta acctgcaaaa gtgaaagagg aacaaggtga taaataactc 139500tccgtagatg aactgtgctt gtttttacca gtcaatactt aatgagtgac ttgtatactg 139560aacattgcac caaatatttt aggagaatac taaatagctc atagttttaa gatcttaaaa 139620tctaattggg ttgttataac acatgtatag ctctggcatt tatgcatagt ttccaaaatt 139680tggagaaata tttcgagaaa ctgtcactca agaccaaaat ccaaccaact tggaagaact 139740gattagggaa gaaggcaatg tcttagggag ttggggattg ttagaggagt tggcaggaac 139800atttagagaa aaaaaaaaac attgccaaat tgtgttctgc agggtacaaa ttaatatact 139860tgaaaggatg actacatggt caaaagagaa attctgagtt aaacaagtaa aaccacttcc 139920tttagtgcaa ttgcaattag aacctttaaa atggttatgt atgttttcag tttccaaaaa 139980ggaacttatt ggagacagac ccaattctca ctttttggga gaactgggta ggaagggagc 140040tagtacttag aacatatttt gggaaatgct gccccatagg atttgataag gtggatttta 140100tcatgttcag acaaaagatc catttttcat tcagagacta taaagagaaa acatgtaagt 140160agactgagag tctcacaaaa atacattttt ttttttcaaa attgattcca atgaggagga 140220aaaatgggag gcatgtgaag actttgagtg gctgattaaa gcaagctgat aaataccaaa 140280tgtaaaacag atagaagcca agtgctcaac aatgaaagga gcgatatcag gaataatgtg 140340gatgtgtatg agtagtgtca ggtattgttt gggtgttggg gaaaaaatac ttagggcacc 140400aaatgtaatg tttaaaagtt gaagaaaaaa acaaaagcaa ataagaccta atctagatca 140460ttgcttaagg tggcaggcat catattcttg aatgctggag agtaaggaga actattcaat 140520ttataattta ctaccaatgt ctttttcctt ctctctccct gtttttatgt gtgaaaagag 140580caaaatataa atgttggtaa gaatgaatag atattgaaat aggaggctac tctgaatgag 140640ttttatgcca gtccagaaaa attatacctt agagtactca gagaatttgc agttaagatt 140700ctcttgcttg ggatcagctt actgttgaac tttaaaatac tgtagcaaat aggaatggga 140760ccagaagaca gaatgggcaa atgtaatacc aaatttcaaa aacgagagga gagaaaaata 140820tagtttgcaa cctagaaact aatggtcttg gcattgatct tgcgtggttt tctagattgt 140880gacattttaa gtttatttta tcagcattta tcaagaaata gtgatcacgg aagctagtaa 140940ggaatcactt aggcaaaatc atttcagata tatcattttc ttttcttttc ttttcttttt 141000tttttttttt tttgagacaa ggtcttgcta tgttgcccag gctggagtgc agtgatgtga 141060tcatggctca ctacagcctc aaattcccag gcttaggtga ttctcccacc tcagcctccc 141120aagtagctgg gactacaggc atgcaccacc atgcccagct agtttttaaa attttttttg 141180tggagacagg gttttgccat gctgtccggg ctggtctcaa actcccgggc tcatgcgatc 141240cacctgcctc agcctcccaa agtgctggga tcacaggcat gagccaccac acccggtcct 141300catttccttt ttagatagag caagcagatg gctagattag ggaattttga ttctataaag 141360cattttagaa agtcccgtat gatatctgta aaattggtag atttataacc agttaaatga 141420cagtgttggc ctcagaagag gtctctggta gtaagtcata agcaccttgt cctagatctt 141480gacctagtct accttgattc aaatggttaa attacaatgt tgatggcatg catctttgat 141540gtaccaataa caagatacca gagggagagt gaatgtttga tgatggattg aaacaatggg 141600tgaaatttca ttagatataa tttaatagag ataaatggca aaccttacat taggtagaaa 141660aacaattcca tccattcaag atagatttga caaggcttaa caatagcaca tgtgaaaaag 141720aaatatggtt ttagttaact gtgaatgcat agccgttaac agtggaccat ggctgccccc 141780aaatccaatt gcaatcttaa gccgcattaa tagaaatcat atatctagat tgatgatgct 141840gatagttact cccttctagg tacttgccag atctctgttg cagtgttgta ttttgttctg 141900ataattgtat tgtatgacaa accggttgtg gtctggaaga aattatacaa catggtatag 141960ggatttgaaa taatgtttta tatagtagtt ctagactaaa agaaaactct aaggaggaaa 142020gcacatgata ctatctttaa atacttatta agctgtgata aaaaaaaaaa aaacattagg 142080tttgttctac cctaggaaag gatttgttaa tccccccaaa actggaagaa tttcaaggag 142140gtagatttaa tctcactgta aaaccttcta ataattggaa gtaactttca gcagtattaa 142200gttacctccc cagtgctaga gttgtttaga caaaggcttt gttcttccat ttttggaatg 142260ttataaagga aatgtggggt aagggaagta cattgagtac cgtttatgaa aaaatatctt 142320ccagctttga aattccataa ttccttgttt gatggagtat agataaatta cacatgcaat 142380gaagggccat aagtcgagaa agaaattgtt gttgcaggga agcatcagag gtggcatttc 142440caaggagatg gattttgaac aagttgtgta gaaaatggct aggtaaaagt ggtggaacag 142500caaaataatt ttatttatga ccactggtgg attttcttgt gaaaaatctg ccagtgaaaa 142560atgtccttgg agatggagac ttcacagaca agttttgtat atgagataca catgcacaca 142620tacacacaca caaacacaca cattttaaat ttactttttt aagctcaaag aattattcac 142680gttgataatt tttaattttg ttatttaaga acatgaagga agaatgtttc tagtatctat 142740ttcctaagaa aaggaaagca ggaaaggaat catgtcagaa aaatcaactg atggctgggc 142800acagtgcttc atgcctataa tcccagcact ttggaaagct gaggtgggag aatcaatcac 142860ttgagcccag tagttcaaga ccagcctagg catcacaggg agaccccatc tctacaaata 142920ataataataa aaaattagcc aggcatagtg gcatatgcct gtaatcccag ccacttggga 142980ggctgaagtg ggaggattga gcctgcatgg tagaagctgc agtgagccat gattgtgcta 143040ctacactcca gtctgggtga ctgagcaaga cactgtctca gaaaaaaaaa agtttgtctt 143100ctcaagaaaa atcaataagg gatcttatca tcaacataac caaacacata taaacacagt 143160tcatgcaagt gtatgtgtca ttgcttcatt ttcttcattt cacttcacac tggcactgtt 143220cttcagacca gaatttggga accattgagt tcacaagatc ctttgatggg aatttgggga 143280atttaagact tgttctaggt tatactcaat aacagtcaat ttcaaaatct cagtggctca 143340tcgcagcaag catttctttt tccctcacat tacatgaagg ctgcgtgtta gctgctgtgg 143400ctctcttctc cacattctag attggcttca gatctcatct gagatgtgcc cttcttatgg 143460cacaggagta agagatgcta agcaaaacta taaagttacc atgaaagctt ttactcaaaa 143520gtagcatata tcatacctat tcatgttcca cagtccaaag taagtcacat ggccaactca 143580catcaataca gttgagaagt atatgtgtgc gtgtgtgtgt gtgtagcata atatatatat 143640gaggaaagag atttgtgaac aatatatgct acaataaagt catttcagaa gtttttagat 143700gggaaaatga catgatgaaa actaagaaaa acaatatttg cagcaacagt tagtattaat 143760tgacactgag gacattgaaa acaggaagag taacttgagg atagcttatt tcttctcatg 143820
actctgtaac ttcacctaag tatcagaatc ccagtggcaa aatctcagca cagctaatct 143880catgagatat accttcacac ttcatgtcac attatagtga caagtccaag ttgttcaaat 143940ccaagatagc ttcaccttta attacttttt ttttttttgt caccaaacta caagaatttg 144000atcctgtaga ttttgtcggt cagtttctgt gtaagaatac tcagcagtac aaagaaacaa 144060aatattgaaa catgcagcaa cataaatgaa tctcagaatt attatccttt gtgaaagcag 144120tcaaacacaa aagactacat actatatgaa accttttgta agaaattcca gaaaaggcaa 144180actctggtga caagaagcca gtcagtgttt gcctgtggct agagatcatg ggaagggatc 144240agctgcaaat ggacatgagg aaagttttag ggtgatggag atgctgtaga tcttaatcat 144300ggtagtaggt acattttatt caatataaat tatacttcaa tgaaactgga agaaataaaa 144360aaattgtgct ggttagataa gtaaggatca acttcatttt ctgaagatca acagtaaaaa 144420aaggaggaaa aaaatcaata tttccatgtg tctttgaact ttgtgtgtat ctctgtttta 144480ggaacaactt gaaacagcca ggaagtttct atattatgaa atgggctata aatctcgatc 144540agaacagtta acagatcgct ctgaaattag cctactgcct tcagacattg acaggtactt 144600ataataagtg tctatctatc tctctttttt tttggcctat gtaagtatat ttttccatta 144660tttaatcttg tgttccaggt ataagaagag atttcataag tttgatgcag accagaaagg 144720ctttattacc attgttgatg ttcagcgtgt attagaggta attttctttg gttgatgtca 144780gcctctgata ctagaagaat ataaaaatat tagatgtttt tctcatctag ggttttctac 144840aagtaatatt tttgttataa tttttaaaag atttagtaac attagtgaaa gcatatttat 144900aatttttttt cttttttctt tctttttttt tttttttgag acagagtctc actctgttgc 144960ccaggatggt gtgcagtgtt gcacgatctc ggctcactgt aacctcctcc tcctgggttc 145020aagcggttct cctgcctcag cctccagagt agctgggact acaagtgcgc accaccatgc 145080ccggctaatt tttaaaatat tttttgcaga gatggggttt ctccatgttg gccaggctgg 145140tctcgaactc ctgacctcaa ctaatctgcc tgccttggcc tcccaaagtg ctgggattac 145200aggcatgagc ctccttgccc agccaaagca tatgcataat ttttaatcaa actgtattat 145260tctatgcttt tcttttactt agagtatcaa tgtccaaatg gatgaaaata cactccatga 145320aattctaaat gaagttgatt tgaataaaaa tggacaggtt gaactcaatg aatttttgca 145380ggtgagttgt ggtgaaagga aacaaggata tttgctttta tcttttgttt gtcatgatca 145440taaatgcatg ttcaagaatg gatatttaag tccacacaat tgtcagatta ttctgaagaa 145500gaagaaagct cgaagccagc agaggttgat atgtctgccc tcaagtgtaa ggctctactg 145560tggtgcatat tcttttcctt tttttgtata agaaacattc cattatgttg ggcaatttag 145620attaaatcca agttatttat cataaagggt tgaatatgaa gcatgctttt taaagacgta 145680tgccatggca tatcaatgct ggaccttttt cttttgctta cttttctttt tcttttcctg 145740tacttcataa gaggcaacac aagatttcta atcttttatg tagaaaattt ttaaaaagta 145800tggttctatt cactaatgaa tcatattcat ttgagaattt agtgattaga ttctgtcatt 145860ataaagaact agaaaaacat aaggaaggaa acttgtattt tggggtcaag accacataat 145920ttaagtattt ccatttccaa ttgttaattt attcaaggct ctgttgttta atctttgtaa 145980tcatgcagga aataaatttt gttactttaa cttcaaactg gatgctacat atcgtttctt 146040aaaagaaatt tcaacaaacc catacattat ttctaactgt ccatatgact ctgatcatat 146100gaagaatgta ttgggtttcc aagcagctag gtttttttct gtagtgcttc ttgggcaggt 146160atttcaaatg aatgccaggt cttgcttgca ccatggcaat gatgaatgct gaacatgaaa 146220taaattaatg ctggggattt tggtcaatgt cagccctttc ttcactgtgt caagttaaac 146280aaacaagatc aaggcaaagg aaatggtaat tgtctctctg gagatgagct gcagataaga 146340gcattgttga atctaaatgc agaaaatttt cacaggttgt gtccagcatt acaaaaaaga 146400atgtttggtt ctttagctta ctgtttaact aaatacacat tttaaattaa atttaaaatt 146460tatgcttggg aattaaaaca ccatttaatg cattttttgt gtagtttttc tggagaatat 146520tttattgttg ccctcacaat gttagaaaac ttttcttcaa agaaatggcc tgtgcttcat 146580ttcatgtgtt gtaaataaac agcagaattt agggttgaaa gacatggcac tttagaaaca 146640ataagaatct gattttatga atatgctatg tggtttaggt tttaagtaat tttcttgaat 146700gcagttgagt taaaagcagg agataattga aactatctgc aggttttctt gtagagtttt 146760ttttttaaat aattgagtat tttttgtcca attccaaatg aaagggacta ttcatgcttt 146820tactaagaac aaatacacag tgtgattttc aactttaaaa tgctgaccat attcctcatg 146880aaatatattc acacccaaat gttatttttt tcttgtaata tggtttctag atacatgtag 146940tgtttaaaga aaacttttga gtttttagaa gttggcacat catatactgt ctatggctta 147000aaaaacctct agctttgtca gttgtgttag ctctgataaa ctttgcaggt cttcctggaa 147060tcagagacaa agcaataaac aataaattgt tccgtcttta gatgtcagca ttgcttatgt 147120tacatcattc ctcagatgta aagtatattt gaagggtttt tttagttttt ttaaaatatt 147180ttgctcttct gcagactaag agcccatgtt tatcccatta aatatatttt ttgaatatag 147240atacggtttt tcattaatct tcacagaagg atcactatag atatctcatg gttatatgac 147300tttatttcta agtctgggtt ttaatgacac ttttgcatgt aggagtccat ctctaataag 147360caatagcttg tgaaacagct tttaataagg tcatgaaatt ctgatccagc cccttggcag 147420atgtctctga ctcttattca gagctctttt taagcaacgt gtctctatag gctaaaagct 147480ataaccctaa aaatattttg agaatttaca tacatacata gatacatcat atatatatat 147540aatgtgtgtg tgtgtatata tataatgtgt atatatatat gatgtgtgtg catgtgtgta 147600tatatttata tgatgaatag ctaaatcttt tctttttaaa acaaataaaa aagattaggg 147660aagtgagatc aagaagagca aatagttgaa accacatcaa acttgaaaag gaaaaaaatc 147720cctatgctca taattgcaaa gtgccttcca gattaaaggc ctttgcccgc tgcctgcatg 147780taatttcaag actaattctg attttatcca ttggataaaa atgaattagc taaaatctgg 147840gctgtagagt gcttttaatt cttaaggtag atctctgggt ccattttcaa ggaattaaat 147900gtaaattacg ttaaaaagtc tttaaggaat ttggatttag gggactcgaa gttgtcaagt 147960acagtaacat taaaaaaaag ggtagacaaa tataggtcat ggtttgtctt tgttcaatcc 148020ctgagattcc tttttaagat tttaaatgga attaaagatc caatatttga agttgctaaa 148080aaaaaaaaaa aaagtgcctt gagcacatta aaggacaaaa aatgatgcat taagatttgc 148140ttttagaggc agggtgattt actagatgga tgtagttttt gtggcagtgt tgaaatacct 148200gcctcttccc ctcttttgga aacgtttaaa aattagactc ttcttatctg agctgtagta 148260cttgttaagg tttttgatgg agcacttaat tacctctgtc tgctgcaatt ctaacgatta 148320tgatgccatg aagaagagag taagttggcc tgcgttctga atctgttgca tattttctct 148380ttaagctgat gagtgctatt caaaaaggaa gggtatctgg aagccggctt gctatactaa 148440tgaaaactgc agaagagaac ctcgacagaa gagttccaat tccagtggac cgtagttgtg 148500gaggattgtg agtctgggca gtaaatccac agccaacaaa catagaaacg acaaatcacc 148560atgtaacaac cagagatgac tgaaaccact ctgaaataat gaatgtggat agctgccttt 148620tttaacacta gaaaacattc caaaacttta aggtgttggt gtatttgcca gctttatttg 148680ctgtacttta tttgtatttg ccattcagtc tagcttttaa gtatattttt ttctttttct 148740cattttcaat gcacattagt tttgcatctg ttttgtgacc tgttagatgt gacacattct 148800ctttttgttt attcccttat tctaaatgag ttctaaaaac ataatatttt ggcaaaaatt 148860gaaaaaagct ggagacattt tgtgacatgc atacagatag catgtgttat taaaaagagt 148920tgcctattga aatgatcatg tttctggaga aattaagctt atagacagca tgtgttatta 148980aaaagagtta cctattgaaa 149000
權(quán)利要求
1.一種對個(gè)體的高血壓易感性進(jìn)行輔助性診斷的方法，其特征在于，它包括步驟檢測該個(gè)體的GPD2基因、轉(zhuǎn)錄本和/或蛋白，并與正常的GPD2基因、轉(zhuǎn)錄本和/或蛋白相比較，存在差異就表明該個(gè)體患高血壓的可能性高于正常人群。
2.如權(quán)利要求1所述的方法，其特征在于，檢測的是GPD2的基因或轉(zhuǎn)錄本，并與正常GPD2核苷酸序列比較差異。
3.如權(quán)利要求1所述的方法，其特征在于，所述的差異是選自下組的單核苷酸多態(tài)性115330位A→G；135053位G→A；其中，核苷酸位置編號基于SEQ ID NO1。
4.一種檢測樣品是否存在GPD2基因的單核苷酸多態(tài)性的方法，其特征在于，包括步驟(a)用GPD2基因特異性引物擴(kuò)增樣品的GPD2基因，得到擴(kuò)增產(chǎn)物；和(b)檢測擴(kuò)增產(chǎn)物中是否存在選自下組的單核苷酸多態(tài)性115330位A→G；135053位G→A；其中，核苷酸位置編號基于SEQ ID NO1。
5.如權(quán)利要求4所述的方法，其特征在于，所述的單核苷酸多態(tài)性是115330位A G。
6.一種GPD2基因和蛋白的用途，其特征在于，用于制備輔助性檢測的試劑。
7.一種用于輔助性檢測高血壓的試劑盒，其特征在于，它包括特異性擴(kuò)增GPD2基因或轉(zhuǎn)錄本的引物和說明書。
8.如權(quán)利要求7所述的試劑盒，其特征在于，它還含有選自下組的試劑(a)與突變部位結(jié)合的探針；(b)識別突變位點(diǎn)的限制性內(nèi)切酶。
9.如權(quán)利要求8所述的試劑盒，其特征在于，所述的突變選自115330位A→G；135053位G→A；其中，核苷酸位置編號基于SEQ ID NO1。
10.如權(quán)利要求9所述的試劑盒，其特征在于，所述的單核苷酸多態(tài)性是115330位A G。
全文摘要
本發(fā)明公開了一種檢測原發(fā)性高血壓易感性的方法，它包括檢測個(gè)體的甘油三磷酸脫氫酶基因GPD2、轉(zhuǎn)錄本和/或蛋白與正常相比是否存在變異，存在變異就表明該個(gè)體患原發(fā)性高血壓的可能性大于正常人群。本發(fā)明還公開了相應(yīng)的檢測試劑盒。
文檔編號C12Q1/68GK1861805SQ200510025750
公開日2006年11月15日 申請日期2005年5月11日 優(yōu)先權(quán)日2005年5月11日
發(fā)明者張怡, 張奎星, 朱鼎良, 高平進(jìn), 王谷亮, 黃薇 申請人:上海市高血壓研究所, 國家人類基因組南方研究中心